US20170365054A1  Methods and systems for producing an implant  Google Patents
Methods and systems for producing an implant Download PDFInfo
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 US20170365054A1 US20170365054A1 US15613476 US201715613476A US2017365054A1 US 20170365054 A1 US20170365054 A1 US 20170365054A1 US 15613476 US15613476 US 15613476 US 201715613476 A US201715613476 A US 201715613476A US 2017365054 A1 US2017365054 A1 US 2017365054A1
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Abstract
Description
 This application is a continuation of U.S. application Ser. No. 15/072,607, filed Mar. 17, 2016, which is a continuation of U.S. application Ser. No. 14/277,835, filed May 15, 2014, which is a continuation of U.S. application Ser. No. 13/967,738, filed Aug. 15, 2013, which is a continuation of U.S. application Ser. No. 13/616,007, filed Sep. 14, 2012, which is a continuation of U.S. application Ser. No. 13/228,517, filed Sep. 9, 2011, which is a continuation of U.S. application Ser. No. 10/089,467, filed Mar. 27, 2002, which is a U.S. National Stage Entry of International Application PCT/US2000/22053, filed Aug. 11, 2000, which claims the benefit of U.S. Provisional Application Nos. 60/148,275, 60/148,277, and 60/148,393, which were all filed Aug. 11, 1999. All prior applications are incorporated by reference.
 The present invention relates to fabricating implants to replace bony structures. More specifically, the invention relates to a system and methodology for fabricating a “drop in” replacement for a particular segment of missing bony structure, in which the implant fits precisely within the contours of the missing segment and thus minimizes complications during the surgical procedure to install the implant, and subsequently during the healing process. It will be appreciated, however, that the invention is also amenable to other like applications.
 Various systems and methods of fabricating prosthetic implants are known in the prior art. Examples of such prior systems and methods include U.S. Pat. Nos. 4,436,684; 5,274,565; 5,357,429; 5,554,190; 5,741,215; and 5,768,134. Each of these patents, however, suffer from many disadvantages that have collectively limited the usefulness of their methods and implants of the relevant field.
 Although clinical visualization of three dimensional organ surfaces embedded in an imaged volume is now common, segmentation techniques are often ineffective and time consuming. Current three dimensional (3D) surface segmentation is often dependent on the contrast and resolution of two dimensional (2D) boundaries identified by an operator proceeding slice by slice through the image volume. Existing automated 3D surface and volume segmentation algorithms, such as region growing, are most commonly based on voxel intensity, nearest neighbor similarly, and edge strength. An active contours energy minimization approach attempts to automate 2D outline detection. An extension to 3D suggests regions to be segmented by internally enveloping them with threedimensional bubbles. Another method based explicitly on high level volumetric shape features utilizes prior information obtained from previously imaged anatomical objects. This approach is based on prior morphological knowledge and a highly integrated set of model based grouping operations. The use of prior information allows the last method to be more adaptive to an expected surface when it encounters noise due to artifact or nondescript structures such as in thin areas. However, detection of anatomical surfaces as complex as the skull or soft tissue face with prior information alone is computationally expensive. It is likely manual work will be necessary where the signal is too low, especially in regions with high artifact noise or where artifactinterrupted surface morphology may obfuscate a match with database information. The relationship of artifacts to surface morphology is dependent on both surface proximity to the artifactgenerating structure and patient pose. To overcome these limitations, a third approach, combines semiautomatic image segmentation procedures with intelligent scissors. A user steers the image segmentation via live wire and live lane tools through the image volume.
 Current slicebased segmentation methods tend to be time consuming. Imprecision correlates with the numbers of decisions the operator is forced to make. These are concentrated in areas of poor contrast, artifact, or insufficient geometry for the user to recognize local aspects of the patient's anatomy (i.e., the sufficiency of anatomical cues are heavily dependent on structural size and degree of curvature as well as how patient pose affects the representation in tomographic images). Difficulty in making segmentation decisions based on these three (3) types of information may be compounded by a user environment where an image volume is presented as a series of tomographic or projection images that lose resolution when reformatted. In all cases these two dimensional views require anticipation of how the segmented outlines will combine to form the 3D surface that the user wishes to render.
 Deformable curve fitting for edge detection seems less adaptable than manual segmentation for determining the location of anatomical edges. Curve fitting runs into trouble when edges vary in signal strength, requiring multiple initialization and guidance steps to obtain bestfit. An operator familiar with the salient anatomy quickly adapts to provide continuity between areas of material inhomogeneities (i.e., varying pixel intensity) that may signal a break to an edge detection algorithm. It is possible that these edges can be spanned by the use of geometrically ideal shapes or those based on prior information. However, these simple models are less adaptable, or less decisive, than manual segmentation.
 Neural networks, applied to volume image segmentation, are capable of modeling structural components in the image through the processing of the gray scale and spatial information recorded in volume images. They take advantage of the multidimensional nature of medical images. Neural network classification can be either supervised or unsupervised. To date, supervised neural networks have been preferred, despite their dependence on user interaction to train or correct their results. Unsupervised neural networks do not rely on prelabeled samples. Rather, they require careful selection of input events that, when clustered, are useful to the user.
 There is debate as to whether comparison of three dimensional (3D) anatomical shapes should be done on the basis of whole objects or elementally. One conventional method for 3D anatomical surface parsing begins with identification of a specialized class of space curves, which are referred to as “ridge curves”.
 A consensus ridge curve definition would cite a maximum first principal curvature along sharp edges as allowing their representation as a space curve. The curvature found along ridge curves is regular enough that a class of curvature extremal landmarks can be readily detected. These are referred to in the literature as Type II landmarks, which may be extracted manually or automatically. These landmarks may form the initial seed points on which to register a wire frame (i.e., ridge curve and “geodesic” lines) plate. “Geodesic” space curves, a shortest path traveled on a graphical manifold, are useful as a means to link Type II landmarks. The resulting fitted ridge curve and “geodesic” wireframe are used as a means to tile out and parameterize 3D surface images. This toolkit allows parameterized (labeled) surface extractions, multisurface registration, and averaging for cephalometric comparisons of the skull, soft tissue face, and cerebral ventricles.
 In regards to parameterized 3D surface extraction, the toolkit facilitates an initially manual superimposition of a ridge curvebased deformable template to the perspectively rendered surface of interest at the Type II landmarks. However, there is only an approximate tie between the surface tile points bounded by ridge and “geodesic” space curves, and the surface voxels originally identified (segmented) in the raw CT or MR scan volume image data. The “geodesic” criteria are less appropriate and these lines have been renamed “filing curves.” Additionally, the entire surface is encoded as set of Bspline space curves, in order to facilitate whole surface averaging algorithms, (i.e., to average multiple surfaces space curve by space curve). The toolkit links biological labeling (homology mapping) and geometric surface subdivision, resulting in a homology mapped (tile name) parameterized (u,v space of tile) surface.
 The primary basis for positioning multiple biological surfaces for conventional averaging is a specialized class of space curves referred to as “ridge curves”. One definition of ridge curves describes them as biologically significant space curves found along sharp edges, corresponding to first principal curvature maxima. These sharp edges are the most bent part of the surface. Type II landmarks, identified as recurrent curvature maxima (bends), are found along ridge curve space curves. They span these landmarks with what are called “geodesics” in order to create three or four sided tiles (“filing curves”).
 It has been proposed to add tile parameters (i.e., tile u,v coordinates and tile name) to the xyz point coordinates found in 3D skull surface images to produce a featurepreserving average or morphometric comparison of homologous landmark. A geometric subdivision (ridge curve and geodesic tile boundaries) of the surface links biological labels (homology mapping) to file grid (u, v) parameterization of the extracted points (x, y, z). The toolkit begins superimposition of the ridge curve and geodesic (tiling curve) deformable wireframe template with the identification of Type II landmarks. Using a thin plate spline, the ridge curve and geodesic template wireframe is warped to the surface's Type II landmarks. Superimposition of the interceding ridge curve and geodesic wire segments to best position proceeds automatically.
 When too much of the patient is missing from the image, it is useful to match the average to the patient to obtain a reasonable shape to fill the defect. Unlike images of individuals, averages are usually relatively smooth and symmetric (right to left). However, estimating the shape of a defect of large size with an average of too few subjects is also not helpful. In this case, it may be better to use the image of a single relative.
 Homologybased 3D surface averaging requires appropriate superimposition of sample member surface points within a shared reference frame. The toolkit obtains this position in a series of global warping and local unwarping maneuvers. First an average of all sample specimen's Type II landmarks is obtained. Each specimen is then globally warped to these landmarks and then locally unwarped. A rough average of the ridge and geodesic curve sample is produced. Next, because the space curve sample members are fit to the resulting rough average space curve, perpendicular bisectors may be positioned equidistantly along the rough average. An average of the intercepted points appropriately samples the shape of each space curve on the entire surface. The u, v parameters determine the number of points along either side of the four (4) sided tiles and, therefore, contain, u×v internal space curves, which are averaged in the same manner as the boundary curves. This method insures that these sampling planes (perpendicular bisectors) are never parallel to the sampled data.
 The present invention provides a new and improved apparatus and method which overcomes the abovereferenced problems and others.
 A method for determining a shape of a medical device to be implanted into a subject produces an image including a defective portion and a nondefective portion of a surface of a tissue of interest included in the subject. The tissue of interest is segmented within the image. A template, representing a normative shape of an external anatomical surface of the tissue of interest, is superimposed to span the defective portion. An external shape of an implant, is determined as a function of respective shapes of the defective portion as seen in the template, for repairing the defective portion.
 In accordance with one aspect of the invention, a volumetric image of the tissue of interest is produced. In accordance with a more limited aspect of the invention, one of a CT and MR image is produced.
 In accordance with another aspect of the invention, a position for seating the implant into the detective portion is determined. The implant is tapered as a function of a seating strategy and a space available for the implant.
 In accordance with another aspect of the invention, the template is superimposed to the surface of the tissue of interest via warping.
 In accordance with another aspect of the invention, the template is warped to an external surface of the nondefective portion of the issue of interest.
 In accordance with another aspect of the invention, a ridge and tiling curve homology map is produced of another anatomical surface. The average normative shape is produced front control surface image representations of the anatomical surface of interest.
 In accordance with a more limited aspect of the invention, the normative shape is determined from a mirror image of the tissue of interest.
 In accordance with another aspect of the invention, the normative shape is modified as a function of a shape of the nondefective portion of the anatomical surface of interest.
 A system determines a shape of a medical device to be implanted into a subject. An imaging device produces an image including a defective portion and a nondefective portion of a tissue of interest included in the subject. A segmenting means segments the tissue of interest. A template spans the defective portion. The template represents a normative shape of an external anatomical surface of the tissue of interest. A determining means determines an external shape of an implant, as a function of respective shapes of the defective portion and the template, for repairing the defective portion.
 In accordance with one aspect of the invention, the image is a volumetric image of the anatomical surface of interest.
 In accordance with a more limited aspect of the invention, the volumetric image is one of a CT and MR image.
 In accordance with another aspect of the invention, the template specifies a seated edge, which is fixed to the subject, and a surface shape, which is not affixed to the subject.
 In accordance with another aspect of the invention, the template is warped to a deformed bone.
 In accordance with another aspect of the invention, the template is warped to an external surface of the nondefective portion of the tissue of interest.
 In accordance with another aspect of the invention, the normative shape of the template is determined from an additional tissue representative of the hard tissue of interest.
 In accordance with another aspect of the invention, the normative shape of the template is determined as a function of an average shape of the tissue of interest.
 A method for repairing a defect in a tissue of interest included in a subject produces a volumetric image showing a defective portion, which includes the defect, and a nondefective portion of the hard tissue of interest within the subject. The tissue of interest is segmented from the image. A template, having an average shape of the tissue of interest, is warped over the defective and nondefective portions. A shape of the implant is determined, as function of respective shapes of the defective portion and the template. The implant is inserted into the defective portion for repairing the defect.
 In accordance with one aspect of the invention, the implant is cast.
 In accordance with another aspect of the invention, a mirror of another tissue of interest, representing a bilateral mirror image of the tissue of interest, is homology mapped. The normative shape is determined from the mirrored other section of tissue.
 One advantage of the present invention is that it presents an unsupervised neural network algorithm (i.e., self organizing feature map, or SOFM) for automated and accurate 3D surface segmentation, the latter being a necessity for computer generation of cranial implants, diagnostic cephalometrics and image guided surgery.
 Another advantage of the present indention is that user interaction in the SOFM algorithm is limited to determining which segmented surfaces represent the anatomy desired by the user.
 Another advantage of the present invention is that it rapidly assembles automatically segmented, and anatomically valid (i.e., no correction required), surfaces into a surface of interest for rendering of 3D surfaces found in volume images.
 Another advantage of the present invention is that it saves time over manually identifying each pixel or currently available semiautomatic methods.
 Another advantage of the present invention is that it locates continuous surfaces, even in areas of thin structure or imaging artifact (e.g., metallic artifact in xray or CT).
 Another advantage of the present invention is that it combines spatial and intensity information to track 3D surfaces for segmentation.
 Another advantage of the present invention is that it provides a means for anatomically valid segmentation (because 3D surfaces are tracked oblique to the scan plane, irrespective of patient pose (orientation of scan planes are determined relative the patient anatomy) in the scanner).
 Another advantage of the present invention is that it provides a warping method that gives precise, accurate and efficient ridge curvebased template registration for extraction of a parameterized surface.
 Another advantage of the present invention is that a fitted template allows matching of mirrored (filling defect with right or left opposing side data) or average (normative) surfaces and production of averages. The template establishes the homology for these three types of surface matching (i.e., mirroring, onetoone fit, averaging).
 Another advantage of the present invention is that it provides a reduced time for the warping method that is largely due to an automated (simulated annealing) search for ridge and tiling curves in the volume image.
 Another advantage of the present invention is that it provides faster and more reproducible and, consequently, more reliable automated ridge and tiling curve fitting, especially for internal tile points which were previously generated from an error map between an ideal surface and the true surface.
 Another advantage of the present invention, is that it provides a new basis for determining tiling curve and internal tile point location by establishing more reliable homology mapping of these features.
 Another advantage of the present invention is that it provides a template fitting and surface averaging method that will work well on the entire external surface of the body, as well as many internal organs.
 Another advantage of the present invention is that it encodes the entire surface as series of Bspline space curves, including the internal tile points.
 Another advantage of the present invention is that all surface operations (e.g., registration or averaging) may be accomplished with series of homologous space curves, rather than whole surfaces, thereby allowing for computationally less expensive serial or parallel processing of space curve sets.
 Another advantage of the present invention is that it improves the homology assignment of both tiling curves and internal tile points through new surface extraction techniques.
 Another advantage of the present invention is that it uses average 3D surface images to model surfaces in patient images for rapid prototyping of prosthetic implants.
 Still further advantages of the present invention will become apparent to those of ordinary skill in the art upon reading and understanding the following detailed description of the preferred embodiments.
 The invention may take form in various components and arrangements of components, and in various steps and arrangements of steps. The drawings are only for purposes of illustrating a preferred embodiment and are not to be construed as limiting the invention.

FIG. 1 illustrates a subject being imaged using an imaging device in accordance with the present invention, andFIG. 1A illustrates a bone of interest according to the present invention; 
FIG. 2 illustrates a flowchart according to the present invention; 
FIG. 3 illustrates a local negative feedback which excites neuron drop off allowing coordinated input of simulated neuronal field to a neural network; 
FIG. 4 illustrates event inputs to a Network; 
FIG. 5 illustrates a current implementation of a SOFM neural network configuration shown as flow of event space image element input to processor layer of neural network; 
FIG. 6 illustrates Gaussian and Zero crossing curves; 
FIG. 7 illustrates processor network configurations according to the present invention; 
FIGS. 8 and 9 illustrate flowcharts of the segmentation process; 
FIG. 10 illustrates multiple cluster backprojection from map nodes; 
FIG. 11 illustrates a SOFMbased image segmentation and surface generation interface; 
FIGS. 12A12E illustrate a feature cluster map, back projected pixels and triangulated surface tendering;FIGS. 12A and 12B display the resulting triangulated surface rendered soft tissue face and skull.FIGS. 12C and 12D present the voxel back projection from the selected feature cluster nodes;FIG. 12E presents the feature cluster map; 
FIGS. 13A and 13B illustrates a self organizing feature map formation process in which an initial random processor location evolves into the intended gridlike structure as ordering is obtained by the neural network; 
FIG. 14 illustrates both sessions of manual and SOFM segmented surfaces for skull surfaces; 
FIG. 15 illustrates both sessions of manual and SOFM segmented surfaces for soft tissue face surfaces; 
FIGS. 16A and 16B illustrate plots of accumulated differences across all five data sets per slice the soft tissue face surface and the skull surface, respectively; 
FIG. 17 illustrates orthogonal projections of 2D images; 
FIG. 18 illustrates a new ridge curvebased deformable template wireframes superimposed on a soft tissue face graphical manifold, with Type II landmarks, ridge curve arcs, and tiling curses indicated; 
FIGS. 19A and 19B illustrate Type II landmarks of the soft tissue face and boney skull templates; 
FIGS. 20A and 20B illustrate a CT slice highlighting segmented surface voxels of interest which are indexed to vertices (points) in an adjacent rendered manifold surface; 
FIGS. 21A and 21B illustrate a candidate wireframe prior to the warp at lower left and the result of the thin plate spline warp of the wireframe onto the Type II landmarks, respectively; 
FIG. 22A illustrates a space curve c from a sweeping plane positioned at each control point, with tangent vectort , along a normal section of segmented voxel surface s; 
FIG. 22B illustrates a surface normal curvature k_{n }in directiont , where k_{n }attains principal curvature values k_{1 }and k_{2}; 
FIGS. 23A and 23B illustrate a plane constructed from the two endpoint landmarks and an object's center of mass; 
FIG. 24 illustrates a trace along the candidate template's control point to the image surface; 
FIG. 25A illustrates a cross section on the top of the skull and tiling curve control point search planes intersecting that image surface; 
FIG. 25B illustrates a series of search plane iterations; 
FIG. 26A illustrates rotating search planes; 
FIG. 26B illustrates search planes for one ridge curve arrayed along a warped ridge curvebased deformable template space curve; 
FIG. 26C illustrates a final ridge curve registration found by the simulated annealing algorithm; 
FIG. 26D illustrates a candidate tiling curve and its associated search planes; 
FIG. 26E illustrates the fitted control points; 
FIG. 27 illustrates an entire final ridge and tiling curve wireframe superimposed on the image surface; 
FIG. 28 illustrates a flowchart for minimizing the cost function; 
FIG. 29 illustrates a temperature cooling schedule from an initial temperature 100 to a final temperature, −10; 
FIGS. 30A, 30B, and 30C illustrates an overview of a ridge curvebased deformable template registration process in SASE; 
FIG. 31 illustrates three orthogonal projection views showing direct differences between two surface extractions; 
FIGS. 32A and 32B illustrate the soft tissue face surfaces and boney skulls, respectively, obtained via different methods versus the toolkit on the right side; 
FIGS. 33A and 33B illustrate color coded difference images of the skull and soft tissue face surface extraction comparisons with the segmented voxel surface and color coded differences images of SASE skull and soft tissue face surface extractions, respectively; 
FIGS. 34A and 34B illustrate a tile point density; 
FIG. 35 illustrates the toolkit and SASE surface extractions; 
FIG. 36A illustrates an alignment of u and v internal tile curves in a 4 sided tile; 
FIG. 36B illustrates an alignment of u and v internal tile curves in a 3 sided tile; 
FIG. 37A illustrates a soft tissue face ridge curvebased deformable template definition; 
FIG. 37B illustrates a boney skull deformable template definition; 
FIG. 38A illustrates a translation of two shapes into a shared two dimensional common coordinate system; 
FIG. 38B illustrates scaling adjustments between the two shapes; 
FIG. 38C illustrates the rotation, in order to obtain the Procrustes distance (square root of sum of squared) between homologous point landmarks pairs: A . . . D, a . . . d; 
FIG. 39 illustrates a space curve averaging procedure; 
FIG. 40A illustrates a soft tissue face average surface generation; 
FIG. 40B illustrates a skull average surface generation; 
FIG. 41A illustrates a soft tissue face surface average comparison of various methods; 
FIG. 41B illustrates a boney skull surface average comparison of various methods; 
FIG. 42A illustrates a surface average tilebytile color coded error maps for skull and soft tissue face surface extractions; 
FIG. 42B illustrates an SSA surface average tilebytile color coded error maps for skull and soft tissue face surface extraction; 
FIG. 43A illustrates a tile point density for soft tissue face average; 
FIG. 43B illustrates an SSA method tile point density; 
FIG. 43C illustrates a boney skull surface average; 
FIG. 43D illustrates an SSA method skull the point density; and 
FIG. 44 illustrates an intermethod average surface continuity.  With reference to
FIGS. 1, 1A, and 2 , a subject 10, or patient, is imaged using an imaging device 12 in a step A. Preferably, the image is a volumetric image obtained using a computerized tomography (“CT”) scanner according to a preferred protocol. However, other images, obtained using other imaging techniques (e.g., magnetic resonance) are also contemplated. Once the data is captured from the CT scanner 12, it is stored in a processor 14 (preferably, a transportable storage medium) in a step B (although it could be transferred over a network). Then, the processor 14 segments the data in a step C for extracting a region of the image including a target tissue (e.g., liver, lung, or hard tissue such as bone) 16 of interest within the subject; the extracted region 16 includes two (2) portions (i.e., one portion having a defect 18 and one portion 20 without the defect). Preferably, the nondefective portion 20 substantially surrounds the defective portion. An external surface of the extracted region 16 of the image is mapped in a step D. A decision is made in a step E whether to register the mapped data of the nondefective portion of the extracted region to a memory device in the processor 14, which includes normative (average) data for various bones as a function of various factors (e.g., race, sex, age, etc) of the subject. If the data is transmuted to the memory device, the data representing points on the mapped surface of the nondetective portion of the extracted region are incorporated into averaged data for the respective bone based on the various factors of the subject in a step F.  After the image is segmented and mapped, a template 24 is superimposed in a step G, to an external surface of a normative shape of the target tissue 16 (i.e., without the defect 18). Preferably the template is superimposed via warping. However, a best fit process is also contemplated. Use template specifies a “seated” edge, which is fixed to the subject (patient), and the remaining surface shape, which, is not affixed to the subject.
 It is to be understood that the normative shape represents a desired or average shape of the target tissue. Preferably, the normative shape is determined in a bilateral structure from a substantially mirror image of the target tissue (e.g., a defect is present in a left side of a skull and complete data is available for a right (control) side of a skull). However, if too much of the target tissue is missing on the affected side, or the defect crosses the midline in a way that prevents establishing the midline, appropriate normative average surfaces (which, as described below, are already homology mapped) are used. Inthis case, the normative average data is brought in and registered to the homology mapped normative surface surrounding the defect. Once the template is warped, points on the template are mapped, in a step H, to points on the external surface of the normative shape of the bone of interest 16.
 A shape of an implant 26 to be “dropped in” the defect 18 is determined, in a step I, as a function of a difference between the mapped points on the external surface of the target tissue and the external surface of the template. The implant 26 is seated (fit) in the defect 18 of the target tissue 16 in a step J. A fit of the implant (seating and shape relative to surrounding anatomy) is analyzed in order to produce an image description of the best shape for the implant on computer, not manually. That image description is saved in industry standard format, such as stereolithography (STL), and printed on a 3D rendering device (e.g., stereo lithographic printer). The fit is then, accomplished using a tapering scheme based on the seating strategy and space available for the implant.
 If the implant is not produced in biocompatible material, it is cast in an implantable material. It is to be understood that the present methods work with inert casting materials (e.g., bioplastics such as Polymethymethacrylate) or future tissue engineered materials.
 A stereolithography manufacturing method is contemplated for forming the either the implant master shape or the actual implant.
 As described below, the step C of segmenting the image is preferably executed using self organizing feature maps (“SOFM”), which are used for computationally encoding neural maps.
 The SOFM incorporates a modified Hebbian rule. The resulting mapping of data ordering resembles observed neurological patterns. Since then, the SOFM has found widespread use in many practical engineering applications. The SOFM derives primarily from observations of two biologically equivalent computational hypotheses defined as:
 (1) The Hebbian Rule: “A synapse is strengthened or stabilized when pre and post synaptic activations are correlated.” Change in synaptic strength is defined as:

s _{t+}1=s _{t}+α·η (1)  where s_{t }is a connection strength at time t, η is an output (IP in
FIG. 4 ) equal to s_{t}×ξ, ξ is an input pattern, and α is a factor, a userselected value, that is made to decrease for each iteration, to ensure it reaches zero. This, in turn, ensures appropriate synaptic strength formation and system stabilization.  (2) Local Feedback: Each neuron laterally influences the neighboring neurons to either an excitatory or inhibitory state. This results in a competition which enables the formation of a map.
FIG. 3 is an example of an excitation function. The SOFM based 3D surface segmentation presented here extends the concepts of ordered feature maps. In the neural network, each normalized input feature vector is scaled or weighted by multiplication with a userassigned constant vector. This process begins with an event generation step, which results in a pattern ξ={ξ_{1}, ξ_{2}, . . . , ξ_{n}} that is sent to a “resonator” from which potential connection are made. The weights determine feature connection strengths. The scaling results in a vector xi=c*F, with c the feature connection strengths and F the input vector are shown inFIG. 4 . Processor weights adapt to input stimulation and in so doing the neural network organizes an ordered map.  The unit i has connection weights, μ_{i1}, μ_{i2}, . . . , μ_{in}, which are expressed as a column vector mi=[μ_{i1}, μ_{i2}, . . . , μ_{in}]^{T}. The discriminant function of a unit is defined as:

$\begin{array}{cc}{\eta}_{i}=\sum _{j=1}^{n}\ue89e{\mu}_{\mathrm{ij}}\ue89e{\xi}_{j}={m}_{i}^{T}\ue89e\xi & \left(2\right)\end{array}$  For unit k after selecting a winner from each iteration, the weight vector is updated as:

$\begin{array}{cc}{m}_{i}\ue8a0\left(t+1\right)=\frac{{m}_{i}\ue8a0\left(t\right)+\alpha \xb7\left(\xi {m}_{i}\ue8a0\left(t\right)\right)}{\uf605{m}_{i}\ue8a0\left(t\right)+\alpha \xb7{\left(\xi {m}_{i}\ue8a0\left(t\right)\right)}_{N}\uf606}& \left(3\right)\end{array}$  where t is the discrete point at which events are sequentially sent to the network. ∥o∥_{N }is the normalization process, ensuring the weight vectors are bounded. The network's selectivity improves as more input vectors are presented. Network formation requires no initial correlation or order between any of the voxel input data elements. Hence, no initialization or operator assistance is required during feature cluster formation. Iterative steps lead array units to represent the topological order of different combinations of input image features.
 One dimensional feature maps with voxel intensity input data have been extended to include six (6) voxel input data elements (i.e., patterns) in a two dimensional processor and feature map for interactive surface segmentation. The image feature vector is generated for each input voxel, its elements are: (1) A distance from volume centroid to voxel location in three dimensional space; (2) The histogram equalized or normalized voxel intensity; (3) The Gaussian smoothed voxel intensity; 4) The difference of Gaussians; 5) The Laplacian zero crossings; and, 6) Invariant image moments.
 The calculation of VIDE parameters is explained below. In general, intensity information is incorporated to provide voxel differentiation. Spatial distance parameters provide positional information, such as where a surface is located. Gaussian parameters used in self organizing mapbased segmentation provide additional adaptive qualities to the 3D surface detection operation by smoothing artifact noise and compensating for scan protocol sequellae (resolution, pose, and beam strength). The “difference of Gaussian” parameter extends smoothing across a detected surface by introducing effects from local and distant voxels (i.e., the maximum distance between voxels is that of the surface detected but not elsewhere in the image volume). The “zero crossing” parameter provides transitional information important for some kinds of edge detection. The “moment invariant” parameters provide a positional and scale invariant signature of a surface in each region of the image volume; a computationally intensive parameter, moment invariants are initially set to 10 weight (Table I), and raised here only in small, userdefined, regions (e.g., the margins of thin structures) for fine tuning.
 This set of voxel input data element vectors have normalized values of: spatial location via distance transform, voxel density with respect to nearest neighbors via Gaussian and difference of Gaussians, and boundary changes captured via Laplacian zero crossings. The two Gaussian input elements prevent feature inclusion of the spurious background noise commonly associated with 3D images. These parameters are computed during a preprocessing and featureextraction procedure (see
FIG. 5 ). All VIDEs are normalized to a unit range of 0 to 1.0. This bounds the neural network output (i.e., second layer produced from VIDE input).  The voxel input data element vectors are defined as row vectors F_{n}=[E_{dn}, V_{n}, g, g_{d}, Z_{c}, m] with six (6) elements, shown in Table I, where n varies from 1 to the total number of input image voxels. The weighting factors are defined for each element in order to prefer one element over the others. The resultant VIDE vector ξ is defined as:

ξ=κF _{n}=[κ_{1} E _{dn}, κ_{2} V _{n}, κ_{3} g, κ _{4} g _{d}, κ_{5} Z _{c}, κ_{6} m] (4)  where κ_{i }defines the percentage of preference assigned for each VIDE. The differential preference of input VIDE results in associations between neural network processors and the voxel clusters. Table 1 presents the default preferences for each element during experiments.
 The radial distance from the image volume centroid is referred to as the moment of inertia. The image volume centroid is (C_{x}, C_{y}, C_{z}) as determined from:

$\begin{array}{cc}{C}_{x}=\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89e\mathrm{xV}\ue8a0\left(x,y,z\right)\right)/\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89ex\right),\text{}\ue89e{C}_{y}=\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89e\mathrm{yV}\ue8a0\left(x,y,z\right)\right)/\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89ey\right),\text{}\ue89e{C}_{z}=\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89e\mathrm{zV}\ue8a0\left(x,y,z\right)\right)/\left(\sum _{z=0}^{d}\ue89e\sum _{y=0}^{h}\ue89e\sum _{x=0}^{w}\ue89ez\right)& \left(5\right)\end{array}$  where Y(x,y,z) is voxel intensity, d,h,w are number of slices, slice height, and slice width. Euclidean distances are computed from the centroid to each input voxel as E_{d}=√{square root over (((x−C_{x})^{2}+(y−C_{y})^{2}+(z−C_{z})^{2}))}. This distance is normalized as E_{dn}=E_{d}/∥E_{d}∥_{max}.
 This value depends on a prior choice of gray scale histogram normalization or equalization via a lookup table procedure. The value V_{n}=f(V(x,y,z)/∥f(V)∥_{max }is calculated from histogram mapping and normalized with the maximum value obtained from the entire volume. The best results are obtained via gray scale equalization.
 A 3D Gaussian is defined as:

$\begin{array}{cc}G\ue8a0\left(x,y,z\right)=\frac{1}{2\ue89e{\mathrm{\pi \sigma}}^{2}}\ue89e{e}^{\left({x}^{2}+{y}^{2}+{z}^{2}\right)/\left(2\ue89e{\sigma}^{2}\right)}& \left(6\right)\end{array}$  where σ defines the spread of the Gaussian curve as shown in
FIG. 6 . Use of two Gaussian features allows the neural network to produce feature clusters in noisy regions. Precomputed numbers are used to normalize the 3D Gaussian. A kernel is computed from this Gaussian function with prespecified kernel size ranging from 2 to 10. The smoothed voxel intensity results from the convolution of the kernel over the present voxel and its immediate three (3) dimensional neighbors.  This is an extension of the above Gaussian input element with dual σ_{1}, σ_{2 }values, shown separately in
FIG. 6 . This is defined as: 
G _{d}(x,y,z)=∥G _{1}(x,y,z)−G(x,y,z)∥ (7)  where

${G}_{1}\ue8a0\left(x,y,z\right)=\frac{1}{2\ue89e{\mathrm{\pi \sigma}}_{1}^{2}}\ue89e{e}^{\left({x}^{2}+{y}^{2}+{z}^{2}\right)/\left(2\ue89e{\sigma}_{1}^{2}\right)}$  and G(x, y, z) is defined in equation 6. As in Equation 6, precomputed numbers are used to normalize this Gaussian function, A kernel is computed from G_{d}(x, y, z) and g_{d }is (2x) obtained by convolving this kernel with the present voxel and its immediate neighbors (i.e., the 6 directly adjacent voxels).
 Lapiacian Zero Crossings are presented by as:

Z _{c}(x,y,z)=√{square root over ((Z_{c}(x)+Z _{c}(y)+Z_{c}(z)))} (8)  this value is obtained from the Lapiacian computed in each axis in the image volume, where:

${Z}_{c}\ue8a0\left(x\right)={\left(\left(\sum _{m=d}^{d}\ue89e\sum _{m=d}^{d}\ue89eV\ue8a0\left(x,y+n,z+m\right)\xb7\mathrm{sgn}\ue8a0\left(n\right)\right)/L\right)}^{2},\text{}\ue89e{Z}_{c}\ue8a0\left(y\right)={\left(\left(\sum _{m=d}^{d}\ue89e\sum _{m=d}^{d}\ue89eV\ue8a0\left(x+p,y,z+m\right)\xb7\mathrm{sgn}\ue8a0\left(n\right)\right)/L\right)}^{2},\text{}\ue89e{Z}_{c}\ue8a0\left(z\right)={\left(\left(\sum _{m=d}^{d}\ue89e\sum _{m=d}^{d}\ue89eV\ue8a0\left(x+p,y+n,z\right)\xb7\mathrm{sgn}\ue8a0\left(n\right)\right)/L\right)}^{2}$  and, where sgn(n) is the sign of the scalar x. L is equal to (2d+1)d where d is a predefined length; and V is voxel intensity.
 The moment invariant is an image statistic that is invariant to rotation, translation, and scale in a region. Moment invariants are derived from the definitions of moments, centralized moments, and normalized central moments. The image moments are defined as:

$\begin{array}{cc}{m}_{\mathrm{pqr}}=\sum _{x}\ue89e\sum _{y}\ue89e\sum _{z}\ue89e{x}^{p}\ue89e{y}^{q}\ue89e{z}^{r}\ue89ef\ue8a0\left(x,y,z\right)& \left(9\right)\end{array}$  where p,q,r∈{0,1, . . . , l}. If ƒ(x,y,z) is a piecewise continuous function with bounded support in a 3D image, then the bounding is set by an m×x×o voxel array. It is positioned at the selected voxel and bounded by length l. The centralized moment μ_{pqr }is defined as:

$\begin{array}{cc}{\mu}_{\mathrm{pqr}}=\sum _{x}\ue89e\sum _{y}\ue89e\sum _{z}\ue89e{\left(x\stackrel{\_}{x}\right)}^{p}\ue89e{\left(y\stackrel{\_}{y}\right)}^{q}\ue89e{\left(z\stackrel{\_}{z}\right)}^{r}\ue89ef\ue8a0\left(x,y,z\right)& \left(10\right)\end{array}$  where
x =m_{100}/m_{000},y =m_{010}/m_{000},z =m_{001}/m_{000 }(i.e., centroid coordinates) at the point (x,y,z) is called the image region centroid. The normalized central moment is defined as: η_{pqr}=μ_{pqr}/μ_{000} ^{γ} where γ=(p+q+r)/2+1. From the normalized central moments, three (3) orthogonal invariant moments are defined as: 
φ_{1}=η_{200}·η_{020}·η_{002}, 
φ_{2}=2η_{110}·η_{011}·η_{101}, 
φ_{3}=η_{020}·η^{2} _{101}+η^{2} _{011}·η_{200}+η^{2} _{110}·η_{002} (11)  These three orthogonal moment invariants were selected because they are irreducible and distinct. When approaching tissue boundaries the moment invariants may provide information that complements the zero crossing data. The strength of the zero crossing 3D surface signal is expected to be more dependent on the object's pose (i.e., relation of surface orientation to scan orientation) than the moment invariants.
 In the preferred embodiment, the SOFM neural network utilizes 4×4 to 16×16 processor network configurations in either a rectangular or a hexagonal grid formation (see
FIG. 7 ). In a rectangular grid, each processor has four connections to its neighbors. In a hexagonal grid, each processor unit has six connections to its neighbors. The input layer, translates each voxel into a vector containing the VIDEs defined in Equation 4. The input layer has direct connection to all processors in the formative network configuration and is associated with a weight as shown inFIG. 5 . A flowchart of the complete surface segmentation process is shown inFIG. 8 .  The head holder and background noise are separated from the head object in a two step procedure. First, a grayscale threshold is manually set; next, a volumetric region growing method is applied. This removes voxels associated with the head bolder, background noise, and most metallic artifact outside the head; it also limits the voxels sent to the neural network to those relevant to the 3D surfaces internal to the head.
 The randomization of the VIDE vectors in the input event space is necessary in order to obtain an ordered map. Each voxel intensity in the image volume is stored along with its position (i.e., x, y, z coordinates) in a randomly selected entry in a hash table (i.e., a register queue (length N). The random position R_{p }in the hash table is computed as R_{p}=hashTable[length×randNumber], where the hash table is initialized sequentially from 0 to the total number of voxels N. The random number is generated in a range between 0.0 and 1.0. The length of the hash table starts with value N. Subsequently, N decrements for each position computed and is swapped with entries for that position's current length in the hash table. This procedure ensures randomization of feature vectors in the input element event space and, subsequently, the map formation by adapting weights in the self organization process. The position of the intensity in the hash table entry is replaced during map formation with the processor identity; processors represent feature clusters of potential anatomical surfaces.
 With reference to
FIGS. 8 and 9 , weights are randomly initialized around 0.5±x,x∈{0.0, . . . , 0.1}, in a step K, with default initial learning rate and neighborhood excitation parameters, set by the user.  A pattern ξ, a VIDE vector, is presented, and the network output is evaluated in a step L.
 The unit {i,j} with the minimum output is selected and designate as a winner: P_{i,j}_{winner}=∥ξ−W_{i,j}∥_{min }in a step M.
 Weights are updated, in a step N, using a learning rule where neighbor excitement levels are a function of self and neighbor excitation rates

$\phantom{\rule{1.1em}{1.1ex}}\ue89e{\uf603{W}_{i,j}\ue8a0\left(t+1\right)\uf604}_{\mathrm{winner}}={W}_{i,j}\ue8a0\left(t\right)+{\alpha}_{i,j}\ue8a0\left(t\right)\xb7\left[\xi {W}_{i,j}\ue8a0\left(t\right)\right],\text{}\ue89e{W}_{m,n}\ue8a0\left(t+1\right)=\{\begin{array}{c}{W}_{m,n}\ue8a0\left(t\right)+{\beta}_{i,j}\ue8a0\left(t\right)\xb7\left[\xi {W}_{i,j}\ue8a0\left(t\right)\right],\dots \ue89e\phantom{\rule{0.8em}{0.8ex}},\mathrm{if}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e(\left(m,n\right)\in \xb7{N}_{i,j}\ue8a0\left(t\right)\\ {W}_{m,n}\ue8a0\left(t\right)\end{array},$  where N_{i,j}(t) is a neighborhood of {i,j} at time t; α_{i,j}(t), the learning rate for the winner processor; β_{i,j}(t), is the rate at which its excitation influences its neighbors.
 The value of α_{i,j}(t) and β_{i,j}(t) is decreased, and the neighborhood, N_{i,j}(t) is shrunk in a step O.
 Steps KO are repeated for all the VIDE vectors:

W_{i,j}=[w_{1}, w_{2}, w_{3 }. . . w_{6}]i=1,2, . . . , Ij=1,2, . . . , I_{I×I } 
I∈{4 . . . 16}Q _{i,j} =∥κF _{n} ′−W _{i,j}∥ 
ξ=κF_{n}′ 
n∈{1 . . . N}_{N}Q_{i,j}α_{i,j}(t)α_{i,j}(t)β_{i,j}(t)β_{i,j}(t)γ_{i,j}id∈{0 . . . 255}W_{i,j}N.  The SOFM 3D surface segmentation program integrates the SOFM algorithm, volumetric image reading, slice image display and editing, and graphical surface rendering of output feature cluster voxel data (i.e., obtained 3D surfaces).
 The 2D SOFM processor map displayed in the window (
FIGS. 10, 11, and 12 ) allows the user to select voxel clusters associated with each processor. Clicking on a displayed node activates back projection of the associated voxels to a 3D graphics window. Successive feature clusters associated with desired surfaces may be accumulated, saved as a group, and backprojected to the graphics display (FIG. 10 ). At this stage (i.e., selection of map nodes) no surface rendering is provided.  Our tests were with 3D head CT data. The voxel display of these data allows the user to quickly identify the processor nodes associated with skull, soft tissue face, oral, or cervical vertebral surfaces. After having identified the group of processors associated with the desired 3D surface, the voxels are triangulated for 3D surface rendering.
 During the automated image segmentation process an ordering, from an initially random configuration of processors to a state of equilibrium of the self organizing feature map must occur. The weight vector of a processor is given by W_{i,j}=[w_{1}, w_{2}, w_{3 }. . . w_{6}], where i=1,2, . . . , I, j=1,2, . . . , I and I×I, is the number of processors assigned to the network configuration, where I∈{4 . . . 16}. A maximum of 256 processors were chosen, a condition which insures that the identity of a processor is always found in the range of 0 to 255. This allows each processor to be stored within one byte, thereby saving memory for further computation.
 A VIDE is generated from each voxel in the input volume event space hash table and is sent to the processor layer. Once a processor captures a voxel, the cluster identity is placed back in the hash table register replacing the input voxel intensity cell datum. Later it will be displayed as part of a surface with all voxels belonging to this cluster. Voxel intensity information is obtained from the original input image volume via corresponding hash table registers. A processor's computed output is:

Q _{ij} =∥κF _{n} ′−W _{i,j}∥ (12)  where ξ=κF_{n}′ is a VIDE vector, n∈{1 . . . N} and N is the total number of voxels. Among the processors a winner is identified. The distance measure Q_{i,j }determines if it is lower than the initially chosen value. If so, the processor identity changes from its initial state; the new processor will continue to claim the voxel as long as no other processor claims it is the “winner.” The winner successively strengthens its weight vector by a fraction of the input element values sampled by the α_{i,j}(t), a self excitation parameter. The value of α_{i,j}(t) is set and then decreased successively, eventually resulting in no change to the weight vector. This ensures that the network will reach an equilibrium. Another parameter, β_{i,j}(t) influences neighborhood processor excitation and determines how much the winner can excite or inhibit its neighbor's weights. β_{i,j}(t) is assigned a fixed value that decreases over time to avoid wide fluctuations in the organization process. Using neighborhood Gaussian function curves (See
FIG. 3 ), defined by the parameter γ_{i,j}, the number of neighboring processors is successively decreased. This number is initialized with a default value at the beginning. The “winner” processor takes the current input voxel to its cluster along with its spatial coordinates. This update replaces the processor's identity, id∈{0 . . . 255}, with the voxel density currently in the register.  Prior to the mapping process the weight vectors, W_{i,j }of each processor, are initialized with random values. Therefore, the initial topology of the axis spanned by connection strength between any two of the VIDE elements is unorganized. This is shown in
FIG. 13A . The processor's physical connections remain constant throughout the process, (i.e., processor 1's connections to processor 2 remain the same). However, the mapping topology with respect to each processor's connection strength changes throughout this process. The random starting topology, shown inFIG. 13A , is drawn by plotting each processor's weight vector, chosen two weight elements at a time, onto two dimensional axes. The initial pattern has no meaning, however the final pattern obtained presents processors (feature clusters) containing voxels representing 3D surfaces and similar 3D surfaces will be shown in the same SOFM map region. We have chosen voxel intensity and Euclidean distance and their corresponding members in processor weight vectors to plot the map topology inFIGS. 13, 10, and 11 .  After N input VIDEs are fed through the input layer, producing each processor's final weight vector, the network adopts, approximately, the original “flattened” processor grid topology. The axis of the grid is spanned by processor connection strength values. This grid is a 2D projection image of the feature cluster VIDE hyperspace. An example is seen in
FIG. 13B .  SOFM feature vector adaptation results from two basic mechanisms: (1) Identifying a “winner” and allowing it to modify it's connection strengths; (2) allowing the winner to selectively excite/inhibit its neighbors. The first mechanism ensures connection strengths that reflect input event values. The second mechanism ensures that related processors, especially those representing anatomical surfaces, are mapped adjacently. the observed surfaces, displayed as point clusters from a set of neighboring processor nodes, illustrate the second mechanism in
FIG. 10 .  The voxels associated with the winners are clustered and classified as separate groups. These clusters are identified through the processors identity, a unique value which is stored in the hash table during the self organization process. The resulting clusters capture continuous anatomical surfaces [
FIG. 10 ] that exist in the 3D image volume as an intrinsic model of the VIDE event space. This approach differs from physically based models (prior information) or userdefined (algorithm assisted or manual selection) segmentation methods that rely on information extrinsic to the image volume. Our method adaptively locates surfaces captured in the image volume using voxel data intrinsic to the image volume. We awoke the user to verify and determine the surface identity. This introduces supervised learning to the unsupervised neural network algorithm.  Two different types of surface rendering methods (i.e., triangulation of voxels associated with selected feature clusters) have been implemented. The first approach is primarily a contouring of the outermost voxels. Assuming the volume contains a thin boundary surface, all cells on the surface are classified as useful vertices. Each slice is divided into four quadrants, and each quadrant is swept by 256 lines from its outer most volume boundary to the slice image centroid. In each ray, the first encounter with the boundary surface is defined as a boundary vertex. The remaining sweeps obtain subsequent vertices in each slice, resulting in a closed curve that traverses all of the detected slice vertices. Since the fine sweeps are produced at half degree angular rotation intervals, the points generated are aligned in the correct neighborhood. Next, four sided polygons are generated from all quadruples of neighboring vertices in a pair of consecutive closed curves. These polygons are degenerated to two triangular faces. This results in a complete triangulated surface of the segmented image object. This procedure is extremely fast and well suited to display simple surfaces such as the soft tissue face (see
FIG. 12A ). However, views of objects with complex topology, such as the skull, provide unsatisfactory results.  A second approach is taken to rendering complex surfaces such as the skull. We used a known method of decomposing volumetric data into tetrahedra. This approach takes two adjacent slices and traverses neighboring voxels eight at a time, treating them as a tetrahedron. The decomposition of tetrahedra produces the oriented cycles necessary to represent triangular faces of an isosurface. This approach yields, for example, a skull surface (see
FIG. 12B ). We also compared the Doi and Koide tetrahedral decomposition to the marching cubes algorithm. Both algorithms use bilinear interpolation to compute partial voxel values. Computation of a complete skull surface rendering took approximately 3 to 4 minutes as opposed to 2 to 3 minutes, on an SGI Octane workstation.  The user can combine the feature clusters associated with multiple SOFM processors. If unexpected holes are seen in the surface, perhaps due to partial voluming “drop out” of thin structures, the volume image region immediately around the missing area may be selected as a new region of interest (ROI). A new high resolution processor map of voxel clusters is generated for this zoomedin ROI, allowing careful surface assembly in regions of thin bone or skin.
 This error results in surface “drop out” and is a well known cause of surface inaccuracy in 3D CT visualization of thin bone segments. The problem becomes more difficult where bone voxel intensities are reduced to tissue levels. Region growing and manual intensity thresholding techniques for isosurface generation cannot overcome these errors. We find that selecting a region presenting thin bone segments and assigning higher preferences to VIDEs 5 and 6 (i.e., Laplacian zero crossing and Invariant image moments) allows a local SOFM map to capture more of the desired voxels representing surfaces formed by thin bone. For example, the computationally expensive moment invariant was set at 10 weighing on this initial pass, then elevated to 4060% in userdefined regions around thin bone structures such as the infraorbital foramina and orbital walls (
FIG. 14 ).  The SOFM program was tested with five 3D CT data sets (B2537, B2621, B3037, B3095, B3195) provided by a recall of subjects. All subjects are above 60 years in age, female, have dental fillings, and often have a large oral prostheses (dentures). One operator (AM) segmented all the images twice by two methods: (1) slicebased methods (i.e., intensity thresholding, manual pixel selection, and 2D and 3D region growing) in the AUTO program, and (2) Self Organizing Feature Map. Both the slicebased and SOFM tools provide orthogonally reformatted views of the original image and segmented pixels. More than a day passed between each segmentation session. Intraoperator variance (precision), using either segmentation method, was measured between the two segmentation attempts. In manual segmentation, skull and soft tissue face segmentations were separate activities. In SOFM experiments, the same ordered map was used to produce the skull and soft tissue surfaces in a single attempt.
 The original, unsegmented CT data sets are 512×512×115 in dimension with 1,904 mm slice thickness and 0.5 mm pixel resolution. This scan protocol was chosen to provide accurate source data for boney skull and soft tissue face isosurface images. This data was reduced to 256×256×115 in dimension with 1,904 mm slice thickness and 1 mm pixel resolution. This resolution is sufficient in terms of the resulting surface segmentation and saves memory resources on the computer.
 Five measures were used to determine both intraoperator withinmethod variance and betweenmethod variance in the resulting segmented images. The former measures how reproducible the segmentation is; low variance implies high precision. The latter measures how different the results of the two methods are. We expect the differences to be local to regions of thin structures or metallic artifact where qualitative inspection suggests the greatest benefit from SOFM segmentation. These five measures are: (1) Visual comparison of the skull and soft tissue face surfaces obtained; (2) Difference of the overall volume of the segmented image; (3) Two dimensional difference images computed slice by slice for each slice image within the volume; (4) Three dimensional difference images viewed from three orthogonal eye points; and (5) Procrustes superimposition of manually located, highly reliable, single point anatomical landmarks. The last measure is similar to a qualitative visual determination of the completeness of anatomical features seen on the segmented surface. Clearer features will result in more easily, and more repeatably, detected anatomical landmark coordinates by trained workers.
 The left hand side of
FIGS. 14 and 15 show both manual slicedbased segmentation attempts. The right hand side shows both SOFM segmentation results. SOFM segmentation of soft tissue face recovers thin tissue structures around the nostrils, lips, and ears, producing an improved image. There is also dramatic improvement in reducing the effect of the metallic (dental filling) artifact around the lips. Note the variable posterior sagging of inframandibular soft tissue due to the affects of gravity on the supine individual within the head holder. SOFM skull segmentation (FIG. 14 ) improves areas of thin bone around the infraorbital and mental foramina and the thin posterior and medial orbital walls. These visual results indicate that the slicebased segmentation does not allow as effective a search for these surfaces.  Table II present volumetric differences between the soft tissue face obtained between an average of multiple attempts and each attempt in both manual and SOFM methods and between the two method averages. Table III presents the same data for the segmented skull image volumes. The difference image volumes were computed by multiplying the total number of voxels in each segmented image by voxel volume (1,904 mm3). SOFM segmentation results show less difference between sessions. Given the additional detail it is not surprising that in comparison with manual slicebased segmentation, SOFM segmentation results in an average of 3.9% more volume for skull segmentation and 0.5% more for soft tissue face segmentation. The higher difference for skull segmentation likely indicates greater reduction of partial volume error.
 This test presents 2D slice by slice difference profiles for each image volume using either segmentation method. These difference profiles result from calculating the absolute difference between corresponding pixels in the homologous segmented slices. The accumulated difference across all five data sets are plotted per slice in
FIG. 16A for the soft tissue face surface andFIG. 16B for the skull surface. Regions where the slices contain thin bone and denial filling artifact require manual pixel selection during manual slicebased segmentation in AUTO. These are areas where the user must make many decisions that are not well replicated on subsequent segmentation attempts. Manual slicebased segmentation results in higher intraoperator variability, Regions with thin soft tissue or bone, or dental artifact, contributed most to the difference plots (FIG. 16 ) between the slicebased manual and SOFM segmentation results, this suggests that the latter was more the reliable method.  This test consists of 3D difference images computed between the volume image resulting from the two segmentation methods. The 3D difference images, seen in three orthogonal projections of 2D images in
FIG. 17 , graphically illustrate the affect of highly variable human decision making in the regions of metallic artifact and thin structures during manual slicebased segmentation. Note that thin areas of the skull surround the pneumatic sinuses (i.e., frontal, maxillary, ethmoid, sphenoid, and mastoid). Areas of thin soft tissue face structures (especially, the nostrils, eyelids, ears and lips) are more fully extracted by SOFM than with slicebased manual methods.  The user was asked to pick reliable anatomic landmarks twice on the soft tissue face and skull surface segmented either manually or by SOFM. These landmarks include 21 on the soft tissue face surface (Table IV) and 26 on the skull surface (Table V). The error found between landmark locations in these two sessions was determined via Procrustes fit. The error reported here is the average value across all 5 superimpositions. We contend that the improved quality of the imaged surface, especially in areas of artifact or thin structures, reduces intraoperator variance (i.e., raises precision) between landmarks picked on two attempts of either the soft tissue face or skull surfaces segmented via the SOFM method; the less ambiguous SOFM surface renderings allow our skilled operator to place these landmarks more precisely.
 Skull and soft tissue face segmentation sessions (AM) were reduced from approximately 3 to approximately 0.5 hours. In either method, the majority of time is spent correcting dental artifact or partial volume error. We are aware that the skull is most often segmented at the CT console by intensity thresholding alone. This method may save time but results in the poor segmentation of thin structures or those near metallic artifact.
 As described below, the step G of warping the template to an external surface of a normative shape of the bone of interest is preferably executed using a Simulated Annealingbased Surface Extraction (SASE) process.
 In order to save time, improve precision (repeatability), and increase accuracy of the extracted anatomical surface, a ridgecurve based deformable template superimposition strategy combines simulated annealing of a ridge and tiling curve wireframe and subsequent surface normal based identification of surface tile points. The annealing algorithm searches the volume image and displays the result to the operator on a graphical manifold rendering of the surface of interest. Unlike energy minimization algorithms, which require a good initial parameter estimation to avoid converging to a misleading local minimum, annealing methods exploit stochastic search methods to locate a global minimum. Several stochastic methods form a basis for simulated annealing. Space curve lifting of 3D surfaces is a goal not uncommon in the field of computer vision. It is merely object recognition followed by shape registration.
 Bspline space curve encoding of labeled surfaces has application beyond surface averaging and craniometrics or image coregistration. We seek highly accurate surface extractions to model cranial prosthetic implants for the skull. Homology mapped, Bspline space curve encoded surfaces prove useful in morphometric, biomechanical, and deformable model assessment of many organ systems. Additionally, parameterized surface extractions, especially of the external body surfaces, have application in animation.
 We chose to use Bspline surface encoding because it provides a smooth surface approximation, reduced data set, and easier assignment of labels to various component (see Appendix 2 for Bspline curve and surface construction). We have reimplemented skull and soft tissue face ridge curvebased deformable templates in this fashion.
FIG. 18 shows one of these new ridge curvebased deformable template wireframes superimposed on a soft tissue face graphical manifold, with Type II landmarks, ridge curve arcs, and tiling curves indicated.  Since ridge curves rarely intersect, additional space curves are necessary to tile the surface with a single enclosing wireframe. In order to identify a unique demarcating line within a search space we define tiling curves as space curves that: (a) traverse a low curvature area (i.e., flat surface); and (b) are weighted to pass close to a plane passing through the surface between the tiling curve end points and the center of mass of the entire image surface (See Sections II.2.C and II.2.G).
 Initially, we approximate tiling curves as straight lines between endpoint landmarks. In
FIG. 18 , Bspline ridge and tiling curves are indicated by dotted lines while their control points are shown as small black crosshatches. The Type II landmarks are shown as large black crosshatches.  As one draws either a ridge or tiling curve, a corresponding Bspline curve is fit to the surface via global curve interpolation to a set of control points sampled from the user drawn space curve. The Bspline interpolated 3D points collected are resampled and indexed to vertices on the graphical manifold surface and assigned a control point vector, P_{1}. In the case of ridge curves, our resampling maintains the curvature information along ridge curves when determining howto space the control points. On the other hand, tiling curve control points are equidistantly spaced. The curve smoothness, set by the operator, further determines redistribution of control points, if necessary. Knotvectors and knotspacing require a Bspline interpolation function assignment for each space curve, by the operator during the template creation process.

FIGS. 19A and 19B display the Type II landmarks and Appendix 1A and 1B list the nonlandmark components of the soft tissue face and boney skull templates, respectively. The soft tissue face template consists of 56 Type II landmarks, 103 space curves (54 ridge curves and 49 tiling curves), and 44 surface tiles [FIG. 19A , Table VI]. The skull template consists of 54 Type II landmarks, 104 space curves (40 ridge curves and 64 tiling curves), and 47 surface tiles [FIG. 19B , Table VII].  The 3D surface input we use results from segmented volume images. The segmented voxels are rendered as a graphical manifold. Segmentation occurs via an unsupervised neural network program. The graphical manifold is built with subsampled voxels to provide an interactive context to the operator.
 The simulated annealing ridge curvebased deformable template superimposition algorithm evaluates features found in the volume image, not on the graphical manifold surface. The unsubsampled volume image provides additional cues for best locating ridge or tiling curves. The result of the search is displayed on the graphical manifold surface where the operator can make manual corrections to algorithmic insufficiencies. The CT slice shown in
FIG. 20A highlights the segmented surface voxels of interest which are indexed to the vertices (points) in the adjacent rendered manifold surface (FIG. 20B ).  During loading of the graphical manifold surface data, a 3D lookup table (i.e., geometric hashtable) of spatial x,y,z and curvature metrics (Listed In Table 8) corresponding to each vertex is generated. This provides the simulated annealing based surface extraction complete indexing between, the rendered graphical manifold and the source volume image.
 The first step in the Simulated Annealingbased Surface Extraction (SASE) process is the operator's manual location of the Type II landmarks on the graphical manifold surface. These landmarks attach the ridge curvebased deformable template to the graphical manifold surface via a thin plate spline warp.
FIG. 21A shows a candidate wireframe prior to the warp at lower leftFIG. 21B shows the result of the thin plate spline warp of the wireframe onto the Type II landmarks. Some of the landmarks (large crosshatches) are visible. Note how the candidate wireframe undulates in and out of the surface.  Simultaneous to loading of the graphical manifold data and lookup table generation, a set of surface geometry features are generated for use in the simulated annealing procedures. A method is known for computing principal directions and principal curvature values (
FIG. 22 ) for any point on the segmented image surface directly from the input volume image data. In addition, the principal normal and tangent vectors are derived at the same time. These latter parameters, in addition to providing the basis for the simulated annealing process cost functions, are also used to bound the search space in the volume image linked to the graphical manifold surface displayed for the operator.  The search for volume image principal curvature normal vectors and principal curvature tangent vectors occurs initially at a point, I, a control point found on the Bspline space curve, defined at location {x_{0}, y_{0}, z_{0}} to have normal vector {right arrow over (N)} on the volume image surface S defined as I(x,y,z)≡I(x_{0}, y_{0}, z_{0}). I lies in the same direction as the curvature gradient (i.e., changing surface normals) of the surface voxel data:

∇I=(I _{x} ,I _{y} ,I _{z})({right arrow over (N)}=(∇I)/∥∇I∥) (EQ 13)  In order to evaluate the local surface curvature we consider a space curve C from a sweeping plane positioned at each control point, with tangent vector {right arrow over (t)} along a normal section of segmented voxel surface S (
FIG. 22A ). The normal vector at any point along C is N=(n_{x},n_{y},n_{z}). Differentiation of ∇I·t=0 yields surface normal curvature k_{n }in direction {right arrow over (t)}, where k_{n }attains principal curvature values k_{1 }and k_{2}. Principal directions {right arrow over (e_{1 })} and {right arrow over (e_{2 })} show the path of the principal curvatures (FIG. 22B ).  On any surface, the local curvatures vary between the principal curvatures k_{1 }and k_{2}. Therefore, the curvature parameters we use combine these measures:

K=k _{1} ·k _{2} (EQ 14) 
M=½{k _{1} +k _{2}} (EQ 15)  where K is Gaussian curvature, independent of principal directions and M is mean curvature changes sign with opposing principal directions.
 Twelve feature dimensions are used for simulated annealing registration of ridge or tiling curves (Table VIII). The 3rd order image derivatives are obtained from image derivatives:

$\begin{array}{cc}H=\left[\begin{array}{ccc}{I}_{\mathrm{xx}}& {I}_{\mathrm{xy}}& {I}_{\mathrm{xz}}\\ {I}_{\mathrm{yz}}& {I}_{\mathrm{yy}}& {I}_{\mathrm{yz}}\\ {I}_{\mathrm{zx}}& {I}_{\mathrm{zy}}& {I}_{\mathrm{zz}}\end{array}\right]& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e16\right)\end{array}$  The Partial derivatives of H along each axis are H_{x}, H_{y}, H_{z}:

$\begin{array}{cc}{H}_{x}=\left[\begin{array}{ccc}{I}_{\mathrm{xxx}}& {I}_{\mathrm{xyx}}& {I}_{\mathrm{xzx}}\\ {I}_{\mathrm{yxx}}& {I}_{\mathrm{yyx}}& {I}_{\mathrm{yzx}}\\ {I}_{\mathrm{zxx}}& {I}_{\mathrm{zyx}}& {I}_{\mathrm{zzx}}\end{array}\right],{H}_{y}=\left[\begin{array}{ccc}{I}_{\mathrm{xxy}}& {I}_{\mathrm{xyy}}& {I}_{\mathrm{xzy}}\\ {I}_{\mathrm{yxy}}& {I}_{\mathrm{yyy}}& {I}_{\mathrm{yzy}}\\ {I}_{\mathrm{zxy}}& {I}_{\mathrm{zyy}}& {I}_{\mathrm{zzy}}\end{array}\right],\text{}\ue89e{H}_{z}=\left[\begin{array}{ccc}{I}_{\mathrm{xxz}}& {I}_{\mathrm{xyz}}& {I}_{\mathrm{xzz}}\\ {I}_{\mathrm{yxz}}& {I}_{\mathrm{yyz}}& {I}_{\mathrm{yzz}}\\ {I}_{\mathrm{zxz}}& {I}_{\mathrm{zyz}}& {I}_{\mathrm{zzz}}\end{array}\right]& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e17\right)\end{array}$  Among these 27 third order partial derivatives, ten derivatives are chosen, I_{xxx}, I_{xxy}, I_{xxz}, I_{xyy}, I_{xyz}, I_{xzz}, I_{yyy}, I_{yyz}, I_{yzz}, I_{zzz}, preferring derivatives along the three axes. Essentially the maxima of these ten third order partial derivatives provide an indication of sharp geometric transition. A predetermined interknot spacing restricts any overlap of search space between any two control points. The Type II landmarks are used for ridge curve arcseparation and to anchor tiling curves.
 We use Gaussian and mean curvatures, and ten third order partial image derivatives to obtain maximum curvature prescribing control points along the surface between ridge curve arc endpoints (i.e., Type II landmarks).
 We use the same set of features to position tiling curve control points along lines of minimum curvature. In addition, we use a thirteenth feature, a deviation measure, d_{m}. It is found between the candidate control point normal and the nearest ray within a plane constructed from the two endpoint landmarks and the object's center of mass (
FIG. 23 ). The deviation measure helps limit the tiling curve search space so that no boundaries overlap.  When the template is warped to operator located landmarks on a patient image the candidate space curves connecting the Type II landmarks and their control points may not lie on the image surface. The true surface is located using a trace along the candidate control point normal. Thus, the initial candidate control point is assured to be on the image surface.
FIG. 24 illustrates a trace along the candidate template's control point to the image surface. The annealing process starts with the first point found on the surface as an initial candidate control point.  A search plane is anchored to the candidate wireframe (i.e., ridge curvebased deformable template) and rotates about the axis of that point's surface normal in the volume image. Note that when ridge or tiling curves sit on a two sided tile boundary, these cut planes rotate a full 360 degrees (
FIG. 25B ). The sweep rotation about each control point normal is set to increment a default 45 degrees (operator chooses between 10 and 90 degrees). If search in one direction fails, an opposite direction search is attempted. This is important where a ridge arc is at the surface margin.  The principal directions constrain the search space for each control point along the Bspline space curves. The maximum principal curvature bounds the space in which it is profitable to search for optimal Bspline space curve control points. The search space for each Bspline control point during ridge curve or tiling curve superimposition is defined from the point normals and tangents bounded by the principal directions.
FIG. 25A shows a cross section on the top of the skull and tiling curve control point search planes intersecting that image surface.FIG. 25B shows a series of search plane iterations.  Candidate control points are evaluated during each search plane iteration. Search plane rotations select a randomly located set of M (chosen by operator, default is 2) candidate control points for k search iterations. Random candidate positions are selected from the space curves intersected by the search plane rotations through the image surface. Their features (Table VIII) are computed and evaluated by the cost function in comparison to the current candidate control point position, ζ. The actual candidate locations on the image surface chosen for evaluation via variables r and s using reflection coefficient parameter α:

$\begin{array}{cc}r=\left(\frac{\alpha}{N\ue89e\sqrt{2}}\right)\xb7(\sqrt{\left(N+1\right)}+N1.0& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e18\right)\\ s=\left(\frac{\alpha}{N\ue89e\sqrt{2}}\right)\xb7(\sqrt{\left(N+1\right)}1.0& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e19\right)\end{array}$  where N is the number of dimensions (i.e., number of image features in the case presented). Randomly distributed position vectors (I_{p}) are computed by using r and s as:

Ip=S(h·rand(r), w·rand(s)) (EQ 20)  where S is the search space spanned by width, w, and height, h, computed from the normal and tangent at the template Bspline space curve control point. The rand(r),rand(s) are the coordinates with the search plane. Each position within the search space plane carries a single vector for the three coordinate axes, which is indexed to a segmented surface voxel in the volume image space.
 The probable distribution of positions are subsequently used in finding the best ridge or tiling curve control point candidate. The values for r and s are decreased after each iteration:

r=r−(r/k), s=s−(s/k) (EQ 21)  and successively decreased, as k the current iteration, approaches the maximum number of iterations, k_{max}.
 The cost function evaluates the candidates for Bspline control points intersected by the search plane via simulated annealing. The procedure used here is based on the Metropolis algorithm.
 The value of the ridge curve search space cost function is computed from the twelve features (Table VIII):

$\begin{array}{cc}E\ue8a0\left(\zeta \right)=\sum _{\mathrm{featurei}=0}^{12}\ue89e{F}_{{\zeta}_{i}}^{2}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e22\right)\end{array}$  where ζ is a candidate space curve control point. Since the ridge curve requires maximum curvature values, the inverse of these squared distance feature is minimized during simulated annealing.
 For tiling curves, the cost function is defined as:

$\begin{array}{cc}E\ue8a0\left(\zeta \right)=\sum _{\mathrm{featurei}=0}^{13}\ue89e{F}_{{\zeta}_{i}}^{2}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e23\right)\end{array}$  The curvaturebased features, as well as the deviation measure (d_{m}) are minimized directly as squared distance features for tiling curve control point identification (Table VIII).
 The rotating search planes are intended to identify optimal control point locations to assign to the ridge or tiling curve (
FIG. 26A ). For example, search planes for one ridge curve (i.e., menton_to_l_gonion) are arrayed along the warped ridge curvebased deformable template space curve inFIG. 26B . Here these search planes pass along the candidate ridge curve control point normal superiorly to find intersection with the voxels representing the base of the mandible.FIG. 26C , shows the final ridge curve registration found by the simulated annealing algorithm. Similarly,FIG. 26D shows a candidate tiling curve (i.e., r_ASSZ_to_r_PBP) and its associated search planes.FIG. 26E shows the fitted control points.FIG. 27 shows the entire final ridge and tiling curve wireframe superimposed on the image surface.  Four key elements are considered in our simulated annealing algorithm implementation: 1) The configuration definition; 2) Random candidate generation mechanism (i.e., definition of neighborhoods on the configuration space); 3) A cost function; and 4) A cooling schedule. In our implementation, we have first defined the configuration space with respect to the curvature features necessary to locate ridge or tiling curves on an image surface (Section II.2.C). Second, candidate control points are randomly generated from the search planes (Section II.2.F). Third, standard cost functions for location of ridge and tiling curves have been defined (Section II2.G). Finally, the annealing procedure is described here.
 The annealing algorithm (
FIG. 28 ) attempts to minimize the cost function E(ζ) where ζ is a continuously modified candidate Bspline space curve control point, which lies m a search plane intersecting the surface. A set of cost functions E_{j}(ζ) are constructed from the sequence of template control point deformations ζ^{1}, ζ^{2}, to ζ^{k }starting from ζ^{0 }at the initial ridge curvebased deformable template control point estimate. For each deformation, we have a set of P candidates ζ_{0} ^{k}, ζ_{1} ^{k}, . . . , ζ_{p} ^{k }within multiple search plane in current iteration, k. From the initial candidates, the best candidate ζ^{k }is selected. 
ζ^{k}=ζof ∥E _{j}(ζ^{k})∥_{min} (EQ 24)  as k approaches the maximum number of iterations the cost function E(ζ) reaches a stable control point ζ^{s}. Simultaneously, the temperature, T_{k}, a component of the annealing procedure, is set to decrease monotonically. This parameter limits the search area and determines the probability of determining the best candidate between iterations. As the temperature lowers, the search area and number of probable candidates, narrows. At each iteration, k, the algorithm determines a new value of the Bspline control point deformation parameters, based on the previous value (i.e., ζ^{k−1}) and compares it with the present value.
 ζ^{k }is determined with probability p_{k}−1, which are defined as:

$\begin{array}{cc}{\zeta}^{k}=\{\begin{array}{c}\hat{\zeta}\in {p}_{k}1\\ {\zeta}_{k1}\notin {p}_{k1}\end{array}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e25\right)\\ \mathrm{pk}1=\mathrm{min}\ue8a0\left(\mathrm{exp}\ue89e\left\{\frac{\left[E\ue8a0\left(\hat{\zeta}\right)E\ue8a0\left({\zeta}^{k1}\right)\right]}{{T}_{k1}}\right\},1\right)& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e26\right)\end{array}$  T_{k }is a monotonically decreasing temperature sequence, E(ζ) is the cost function computed at the current position I, and E(ζ^{k−1}) is the cost function computed in the preceding iteration.
 The temperature schedule T_{k}[34]:

$\begin{array}{cc}{T}_{k}={{T}_{0}\ue8a0\left(\frac{{T}_{f}}{{T}_{0}}\right)}^{\frac{k}{{k}_{\mathrm{max}}}}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e27\right)\end{array}$  where T_{0 }is initial temperature, T_{f }is final temperature, and k_{max }is the number of maximum iterations allowed.
FIG. 29 presents the temperature cooling schedule from the initial temperature 100 to the final temperature, −10. We set the number of iterations to 120 for our initial testing (see Section 4). 
FIG. 30 presents an overview of the ridge curvebased deformable template registration process in SASE. Manual editing of control point location is provided in the event that the simulated annealing algorithm fails to register a ridge curve arc to the operator's satisfaction. The operator may also locally vary the search parameters (i.e., sweep rotation degree, search plane height and width, or annealing temperature).  The registered Bspline control points are interpolated to produce a set of tile boundary points (i.e., smooth ridge or tiling curves). We set the default number of tile boundary points for each space curve to 50, assuming this would be adequate in ail cases. Each tile, therefore, consists of 2500 parametric points. Our soft tissue face ridge curvebased deformable template consists of 44 surface tiles [Tables VI and VII], 106376 tile points. Our skull ridge curvebased deformable template consists of 47 surface tiles [Tables VI and VII], 113334 tile points. Note that some of the tiles are constructed with three bounding curves and the fourth side is a degenerate endpoint of the first and third sides. Since they are redundant, we can reduce the number of tile points encoded somewhat from the fully quadrilateral tile case of 110000 (soft tissue face) and 117,500 (skull).
 The trace of I_{n}={I_{nx}, I_{ny}, I_{nz}} provides the path along each tile boundary point normal to the voxel representing the segmented surface in the volume image. The position of the detected volume image surface point along the directed normal replaces the computed space curve point.
FIG. 30A shows tiling space curve points registered onto the volume image surface via reference to the linked graphical manifold image. The surface normal at each point is seen as a single perpendicular line.  For each computed tile boundary point in a ridge curve or geodesic Bspline, a nearest point is located on the segmented surface voxels. The surface normal vectors, at current curve point I are taken as the average of the local normal and interpolated end point normals:

I _{nx}=(((I _{x} −I _{cx})/xsize)+(e1_{nx}·(1−p)+e2_{nx} ·p))/2 (EQ 28) 
I _{ny}=(((I _{y} −I _{cy})/ysize)+(e1_{ny}·(1−p)+e2_{ny} ·p))/2 (EQ 29) 
I _{nz}=(((I _{z} −I _{cz})/zsize)+(e1_{nz}·(1−p)+e2_{nz} ·p))/2 (EQ 30)  where xsize, ysize and zsize are the width, height and slices in the volume image. I_{cx},I_{cy},I_{cz }is the midpoint of each coordinate, computed during the loading of a graphical manifold produced from the external tile boundary points. The I_{cx}, I_{cy }and I_{cz }are:

$\begin{array}{cc}{I}_{\mathrm{cx}}=\frac{1}{T}\ue89e\sum _{i=1}^{T}\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e{V}_{\mathrm{ix}},\phantom{\rule{0.8em}{0.8ex}}\ue89e{I}_{\mathrm{cy}}=\frac{1}{T}\ue89e\sum _{i=1}^{T}\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e{V}_{\mathrm{iy}},\phantom{\rule{0.8em}{0.8ex}}\ue89e{I}_{\mathrm{cz}}=\frac{1}{T}\ue89e\sum _{i=1}^{T}\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e{V}_{\mathrm{iz}}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e31\right)\end{array}$  where e1_{n}, and e2_{n }are the tiling space curve end point (i.e., Type II landmarks) normals, and p is the distance ratio of the current position I to the nearest space curve end point.
 The internal surface tile point vertices are determined via bilinear Coons surface interpolation (equation 16). The Coons surface is converted into set of interior tile Bspline space curves along u and v parametric directions. The normals of each surface tile point are computed (equations 16, 28, 29, and 30). These normals are used to index the tile points to the volume image surface voxels (
FIG. 30B ).  We compute the normal at each parametric surface tile point by directing a trace from the u, v location calculated from the Coon's surface to the voxels representing the segmented surface in the volume image. The detected volume image surface point replaces the Coons surface tile point.
FIG. 30C shows the extracted surface as it is displayed on the graphical manifold for the operator's approval.  The SASE 3D surface extraction program was written in C in the Silicon Graphics Unix (IRIX) environment and tested on a SGI Octane workstation (R10000 CPU, 256 Mbyte RAM). User interaction occurs via X11/Motif protocols.
 We tested the SASE program with five 3D CT data sets (B2537, B2558, B2621, B3095, B3195) provided by a recall of subjects to the BoltonBrush Growth Study Center at Case Western Reserve University. All subjects were females above 60 years in age. The boney skull and soft tissue face surfaces were segmented in a separate program. We measured the precision of our ridge curvebased deformable template registration and surface extraction by repetition. We compared both our surface extraction and that obtained by the toolkit to the segmented surface voxels in the volume image to test intermethod accuracy.
 Intraoperator precision was tested by repeating the SASE template registrations and surface extractions. Direct differences between the two surface extractions are projected into three orthogonal views (
FIG. 31 ). Although, differences were minimal, most were localized to the oral and orbital regions. The oral region imprecision may be due to dental artifact. The orbital region imprecision may be due to surface ambiguity in thin bone structures. Operator manual effort in correcting ridge curve extraction varied requiring between 10% (soft tissue face) and 30% (skull) of the total surface extraction time in the SASE method. No tiling curve correction was necessary. We also computed the square root mean of sum of squared distance between all tile points (Table IX).  The left side of
FIG. 32A shows the soft tissue face surfaces obtained via the SASE methods versus the toolkit on the right side.FIG. 32B presents the same comparison for the boney skull.  The same segmented surface voxel volume image data were used for template registration and surface extraction by both the toolkit and the SASE program. This provides a baseline to compare how accurately the extracted surfaces match the volume image surface.
 The toolkit generates an initial graphical manifold via the Wrapper and then converts it to Alligator/Winged Edge format. The Toolkit's Alligator/Winged Edge graphical manifold is subsampled, first, during surface rendering from the segmented voxel step, introducing an error on the order of ±1 to ±3 mm for a constant subsampling rate of 2×2×2. Second, a wireframe is nudged into best position on this graphical manifold by interpolation. This step increases the error at each point by ±1 to ±3 mm depending on the variable level of parametric smoothing that the program automatically applies. The SASE surface extraction program indexes (links) the vertices of a less subsampled graphical manifold, used for operator interaction and approval, to the original segmented volume image voxels, where wireframe superimposition operations begin.
 In our second intermethod test, the tile points in surfaces extracted by both methods were mapped to the segmented voxels in their shared source volume image space. The distances between the extracted surfaces and the nearest surface voxel in the volume image along the surface tile point normals were computed. Table X lists the square root of sum of squared distances for each method for all five CT data sets and both surface extraction methods. The surfaces, deviated from the original image voxels by an average of 6 mm for both the skull and soft tissue face surfaces. The SASE method average error was 0.47 mm for the soft tissue face and 0.46 mm for the boney skull (i.e., pixel resolution).
FIG. 33A shows color coded differences images of the skull and soft tissue face surface extraction comparisons with the segmented voxel surface. Similarly,FIG. 33B shows the same color coded difference images of SASE skull and soft tissue face surface extractions. The error is scaled from lower difference in blue to larger differences in red. Note that despite higher sampling density the internal tile points are as true as the tile borders in the SASE extractions. Table XI presents the same results tile by tile. Note that the larger tiles have the greatest error in the toolkit extractions, especially on the calvarium. 
FIG. 34 shows the tile point density. The toolkit extracts fewer points than the SASE program.  Continuity of surface curvatures across surface tile boundaries is a critical rendering tissue for parameterized ridge curvebased deformable template surface extraction. The toolkit interpolates surface normals providing smooth boundary transitions (i.e., no seam).
FIG. 35 displays the toolkit and SASE surface extractions. The SASE program avoids interpolation, using the unaltered, detected segmented voxel surface normals.  As seen in
FIG. 32 , the toolkit tends to produce a bowing (see chin cleft, reduced nasal aperture, and reduced orbital aperture) of some ridge curves. This is primarily due to the interpolation process for surface tile boundary points beside Type II landmarks.  Skull surface extraction with the toolkit for an experienced operator requires 23 hours. In the SASE interface, the operator may extract a skull surface in less than one hour, including the manual effort in correcting 10 to 30% of the automated ridge curve extractions.
 We use a semiautomatic surface parameterization program, Simulated Annealing Surface Extraction (SASE), to homology map the segmented volume image surface. The SASE surface parameterization occurs via superimposition of a ridge curvebased deformable template to each sample member's surface of interest. The first step in the SASE method is manual location of Type II landmarks on a graphical manifold representation of the segmented surface voxels. Second, the candidate ridge curvebased deformable template is warped to these landmarks as in the toolkit. Finally, ridge and tiling curve superimposition occurs via a simulated annealing algorithm which primarily evaluates surface curvature information found in the unsubsampled volume image data. The superimposed ridge curvebased deformable template defines a homology mapped parametric surface that is encoded as a series of Bspline space curves. The toolkit does not use volume image data to superimpose ridge curvebased deformable templates, relying instead on the operator to manually nudge the ridge curve deformable template onto a graphical manifold.
 A Bspline space curve may be recorded as a set of 3D {Q_{k}}, (k=1, . . . , n) points incrementally stacked along a sweep in 3D space represented as a pth degree nonrational Bspline. By assigning a parameter value, u_{k }to each {Q_{k}}, with an appropriate knot vector U={a, . . . , a, u_{p+1}, . . . , u_{m−p−1}, b, . . . , b} where a and b are initial and final conditions (i.e., 0 . . . 1), and m is an ascending function, a Bspline space curve is:

$\begin{array}{cc}{Q}_{k}=C\ue8a0\left({u}_{k}\right)=\sum _{i=0}^{n}\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e{N}_{i,p}\ue8a0\left({u}_{k}\right)\ue89e{P}_{i}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e32\right)\end{array}$  where N_{i,p}(u_{k}) is the pth degree Bspline basis function. These basis functions define the nonperiodic and nonuniform knot vector, U, and control points, P_{i}.
We set the degree of interpolation components as 3, and order 4 (degree+1) for all space curves. Bspline basis functions of the 3rd degree are defined as: 
$\begin{array}{cc}{N}_{i,0}\ue8a0\left(u\right)=\{\begin{array}{c}1,\dots \ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\dots \ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\dots \ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\dots \ue89e\phantom{\rule{0.8em}{0.8ex}},{u}_{i}<u<{u}_{i+1}\\ 0,\dots \ue89e\phantom{\rule{0.8em}{0.8ex}},\left(u\le {u}_{i}\right)\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\mathrm{or}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\left(u\ge {u}_{i+1}\right)\end{array}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e33\right)\\ {N}_{i,3}\ue8a0\left(u\right)=\frac{u{u}_{i}}{{u}_{i+3}{u}_{i}}\ue89e{N}_{i,2}\ue8a0\left(u\right)+\frac{{u}_{i+4}u}{{u}_{i+4}{u}_{i+1}}\ue89e{N}_{i+1,2}\ue8a0\left(u\right)& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e34\right)\end{array}$  from the initial condition, these interpolant variables are computed with the knot vectors.
 We use a chord length method to select each knot vector and a linear averaging method to determine knot spacing along the ridge curve and tiling curve Bspline space curves. Knot density is predetermined by the operator. Since each axis is interpolated separately, as in the 2 dimensional case, the x, y, and z axes can be treated separately to encode each space curve.
 Based on ridge curve and geodesic tile (tiling curve) boundaries alone, a surface tile is defined as a bilinearly blended Coons surface. We extend this definition. We take the internal tile point grid and tie it to the surface voxels segmented from the original volume. Next, we encode these points as row and column Bspline curves in u, v space (
FIG. 36A ). This improved homology mapping prevents overlapping of internal tile points during averaging. Our surface tiles are either three or four sided. In the case of a three sided tile, the fourth side is generated by placing the same number of second side points, with each point filled by a common degenerate value from the intersection of the first and third boundary space curves (FIG. 36B ). Our template uses a bilinear blending function to define location for interior tile points: 
$\begin{array}{cc}Q\ue8a0\left(u,w\right)=\left[\begin{array}{ccc}1u& u& 1\end{array}\right]\ue8a0\left[\begin{array}{ccc}P\ue8a0\left(0,0\right)& P\ue8a0\left(0,1\right)& P\ue8a0\left(0,w\right)\\ P\ue8a0\left(1,0\right)& P\ue8a0\left(1,1\right)& P\ue8a0\left(1,w\right)\\ P\ue8a0\left(u,0\right)& P\ue8a0\left(u,1\right)& 0\end{array}\right]\ue8a0\left[\begin{array}{c}1w\\ w\\ 1\end{array}\right]& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e35\right)\end{array}$  where the functions ((1−u), u, (1−w), w) (i.e., blending functions) blend the boundary space curves to produce the preliminary internal shape of the surface as a cosine function; P is the parametric function representing the interior tile points with respect to parametric values u and w. The surface points generated are fitted to the original image surface via a surface normal trace to the surface voxels segmented from the volume image. The trace modifies the shape of the tile by registering each final surface tile point to the original volume image surface (i.e., segmented voxels). The operator sees the result projected onto a graphical manifold surface. The surface tile points are recorded as rows of Bspline space curves spanning the two pairs of tile bounding space curves (
FIG. 36 ).  The caption to
FIG. 37 lists the Type II landmarks found in both soft tissue face and boney skull surface templates. Our current soft tissue face template consists of 56 landmarks, 103space curves (64 ridge curves and 49 geodesies), and 48 surface tiles (FIG. 37A ). Our current skull template consists of 54 landmarks, 104 space curves (40 ridge curves and 64 geodesies), and 47 surface tiles.  As with the toolkit, the first step in our surface averaging algorithm is the generation of an average Type II landmark configuration. We use the Procrustes superimposition method to produce an average Type II landmark configuration. The ridge and tiling space curve Bsplines making up each sample member are globally warped to this average landmark shape and locally unwarped.
 The Procrustes superimposition method is applied to two landmark coordinate configurations at a time. The two homologous shapes are referred to as landmark configurations X_{1 }and X_{2}, are p×k matrices with kdimensional coordinates of the p vertices of Type II landmarks. Since the geometric operations are linear, this could be extended to include any number of configurations, from X_{2 }to X_{N}. The order in which Procrustes Fitting occurs has no effect on the shape of the final configuration. A Procrustes fit attempts to minimize the sum of the squared distances between corresponding vertices of both configurations.
 The coordinates of X_{1 }and X_{2 }are translated to a shared origin, thereby k dimensions are reduced from the overall pk dimensions. This is achieved by subtracting the respective means of each axis from the X_{1 }and X_{2 }matrices. X_{1 }and X_{2 }are translated to the origin by premultiplying X by (I−P), where P is a p×p identity matrix, and is a matrix with every element equal to 1/p. Therefore I−P is:

$\begin{array}{cc}\left[\begin{array}{cccc}1\frac{1}{p}& \frac{1}{p}& \frac{1}{p}& \frac{1}{p}\\ \frac{1}{p}& 1\frac{1}{p}& \frac{1}{p}& \frac{1}{p}\\ \frac{1}{p}& \frac{1}{p}& 1\frac{1}{p}& \frac{1}{p}\\ \frac{1}{p}& \frac{1}{p}& \frac{1}{p}& 1\frac{1}{p}\end{array}\right]& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e36\right)\end{array}$  and the translation of X_{1 }and X_{2 }to a shared origin is computed as X_{1}′=(I−P)·X_{1 }and X_{2}′=(I−P)·X_{2}. This translation is shown in
FIG. 38A .  A scalar value, called “Centroid Size,” S_{1 }of X_{1}, is calculated by taking the square root of the sum of squared distances of each landmark to the centroid. Centroid size is computed for each axis separately and divided for each control point P:

S _{1}=√{square root over (trace((I−P)X_{1}X_{1}′(I−P)))} (EQ 37)  Similarly, S_{2 }of X_{2 }is calculated. The translated and scaled versions

${X}_{1}^{\u2033}=\frac{1}{{S}_{1}}\xb7{X}_{1}^{\prime}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\mathrm{and}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e{X}_{2}^{\u2033}=\frac{1}{{S}_{2}}\xb7{X}_{2}^{\prime}$  are seen in
FIG. 38B .  The translated and scaled configuration is next rotated to obtain a minimum root sum of square distance between corresponding points. The first step is to rotate all configurations about the first configuration. Next the “rough” average (
FIG. 38 ) position of all the rotated configurations is determined. Finally, all configurations are rotated to the rough average position to obtain the final average configuration. The configuration X_{1}″ is rotated to overlay on configuration X_{2}″ so that the root sum of squared distances between the corresponding landmarks is minimized. First the X_{2}″ configuration is rotated by transposing it and premultiplying it by H, a k×k matrix. Therefore, the X_{2}″ configuration to rotated X_{1}″ is 
{circumflex over (X)} _{2} ^{t} =X _{2}″^{t} ·H (EQ 38)  Where {circumflex over (X)}_{2} ^{t }is rotated X_{2}″ configuration, t is the transpose operator and H is obtained by H=VSU^{t }where V and U are obtained from the singular value decomposition of the product of transpose of X_{1}″ and X_{2}″ as:

X _{1}″^{t} X _{2} ″=UΣV ^{t} (EQ 39)  S is the matrix of diagonal elements of ±1 corresponding to the signs of the Σ matrix in equation 39. The average configuration X_{A }is the average of X_{1}″, {circumflex over (X)}_{2}. The rotation is shown in
FIG. 38C . For the multiple configuration average, we first compute the rough average X_{A }′ by rotating the configurations to the first configuration, one at a time: 
X _{2} ′={X _{1} ″, X _{2} ″}, X _{3} ′={X _{1} ″, X _{3} ″} . . . X _{N} ′={X _{1} ″, X _{N}″} (EQ 40)  where N is the number of configurations to be superimposed. X_{A}′ is computed as:

$\begin{array}{cc}{X}_{A}=\frac{1}{N}\ue89e\left\{{X}_{1}^{\u2033}+{\hat{X}}_{2}^{\prime}+\dots +{\hat{X}}_{N}^{\prime}\right\}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e41\right)\end{array}$  To produce the final Type II landmark and tile boundary Bspline control point average, we rotate each configuration to the rough average configuration, X_{A}, one at a time:

{circumflex over (X)} _{1} ={X _{A} ′, X _{1} ″}, {circumflex over (X)} _{2} ={X _{A} ′, X _{2} ″}, {circumflex over (X)} _{N} ={X _{A} ′, X _{N}″} (EQ 42)  The consensus average landmark configuration X_{A }is computed from {circumflex over (X)}_{1}, {circumflex over (X)}_{2}, . . . , {circumflex over (X)}_{N }as:

$\begin{array}{cc}{X}_{A}=\frac{1}{N}\ue89e\left\{{\hat{X}}_{1}+{\hat{X}}_{2}+\dots +{\hat{X}}_{N}\right\}& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e43\right)\end{array}$  We use a thin plate spline to initially position all sample member surfaces at the average Type II landmark configurations. The first landmark transformations matched homologous regions of two and three dimensional wireframe drawings of organisms. A spatial interpolation routine, widely used in geology and metallurgy, the thin plate spline, was suitably modified and applied to this problem. A thin plate spline warp results in a bending energy matrix describing the shape deformation of the anchor points (i.e., control points and Type II landmarks). We use the thin plate spline to provide a global warp of sample member's ridge, tiling curve, and internal tile 13spline points to the rough average Type II landmark configuration.
 Although the warp is based only on the Type II landmarks shared by all sample members, the appropriate position of all surface tile points is achieved via the mapping function ƒ(R^{2}−R^{2}) such that ƒ(S_{1})=S_{A}, based on the Type II landmarks. This function, a biharmonic equation U(r)=r^{2 }log r^{2}, is a generalized solution. The basis function r=√{square root over (x^{2}+y^{2})} is limited to the two dimensional case. We extend it to three dimensional surfaces as U(r)=r, where r=√{square root over (x^{2}+y^{2}+z^{2})}. A 3D thin plate spline function can be generalized and interpreted visually as {(r, s, t, δ(r, s, t))∈R^{4}∥r,s,t∈R}. If S_{A }is our consensus average Type II landmark reference configuration, with k=3 dimensions and p vertices, (i.e., a k×p matrix), the matrix S_{A }and function matrix K are defined as:

$\begin{array}{cc}{S}_{A}^{\prime}=\left[\begin{array}{ccccc}1& 1& 1& \dots & 1\\ {x}_{1}& {x}_{2}& {x}_{3}& \dots & {x}_{p}\\ {y}_{1}& {y}_{2}& {y}_{3}& \dots & {y}_{p}\\ {z}_{1}& {z}_{2}& {z}_{3}& \dots & {z}_{p}\end{array}\right],4\times p& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e44\right)\\ K=\left[\begin{array}{cccc}0& U\ue8a0\left({r}_{12}\right)& \dots & U\ue8a0\left({r}_{1\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89ep}\right)\\ U\ue8a0\left({r}_{21}\right)& 0& \dots & U\ue8a0\left({r}_{2\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89ep}\right)\\ \dots & \dots & \dots & \dots \\ U\ue8a0\left({r}_{p\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e1}\right)& U\ue8a0\left({r}_{p\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e2}\right)& \dots & 0\end{array}\right],p\times p& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e45\right)\end{array}$  U(r_{ij}) is defined in 3D as the distance from the i^{th }to j^{th }the point in the reference configuration as:

U(r _{ij})=√{square root over ((x_{i} −x _{j})+(y_{i} −y _{j})+(z _{i}−z_{j}))} (EQ 46)  Next, a projection operator L is defined as:

$\begin{array}{cc}L=\left[\begin{array}{cc}K& {S}_{A}\\ {S}_{A}^{\prime}& 0\end{array}\right],\left(p+4\right)\times \left(p+4\right)& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e47\right)\end{array}$  O is a 4×4 null matrix. We use these mapping functions to globally warp a series of ridge curve based deformable template (i.e., ridge, tiling, and internal tile Bspline space curves) wireframes to the rough average Type II landmark configuration via a global warp. Once in position, each space curve is locally unwarped to begin the averaging process.
 In general, thin plate spline warping is defined with a set of homologous points Y, as L^{−1}Y, and the transformation is defined:

$\begin{array}{cc}S\ue8a0\left({X}_{\mathrm{ni}}^{\prime}\right)={A\ue8a0\left[\begin{array}{cccc}1& x& y& z\end{array}\right]}^{\prime}\ue89e{}_{\mathrm{ni}}\ue89e+\sum _{j}^{p}\ue89e\phantom{\rule{0.3em}{0.3ex}}\ue89e{n}_{j}\xb7U\ue8a0\left({r}_{j}\right),\text{}\ue89en=1\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\dots \ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89eN,i=1\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e\dots \ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89eI& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e48\right)\end{array}$  Our space curve averaging is a two step process. The first step begins following the local unwarping of space curves that were globally thin plate spline warped to an average landmark configuration. Then all sample members are locally unwarped and averaged, Bspline sample by Bspline sample (i.e., each set of homologous ridge, tiling, or internal tile curves).
 We use the Procrustes Superimposition method to align the sample member landmark configurations and compute an average configuration from them. Procrustes fitting removes variation due to position, orientation, and scale prior to our calculating a simple average. In equation 45 we see that the computed average configuration is set to X_{A}.
 This is accomplished algorithmically as follows: Let the configurations S_{n }where n varies from 1 to N wireframe space curves (i.e., The wireframe consists of the tile boundary Bspline space curves). X_{A }is an average landmark configuration (Equation 45). Each sample configuration S_{n }is warped to X_{A }landmarks. We refer to this sample of globally warped configurations as Ŝ_{n}. Next, each member of Ŝ_{n }is locally unwarped to the original configuration S_{n }for each space curve. The locally unwarped and assembled space curves are shown in FIG. 39 drawn between hypothetical Type II landmarks A and B. A “rough” average curve is produced as a direct average of the control point coordinates (note: this is possible for Bsplines but not for Bezier splines where the control points may not lie on the encoded space curve). The rough average curve is then resplined. The resplined rough average curve is displayed as a dashed line in
FIG. 39 . The second step is the generation of final average space curves. Sampling planes (perpendicular bisectors) are positioned at regularly spaced intervals along the rough average curve. A control point for each sampling plane is produced from the average of intercepted points. Finally, a Bspline space curve is generated from the average intercept points (FIG. 39 , thick dark line).  The space curve averaging is done separately for each type of space curve (i.e., ridge, tiling, and internal tile curves). Ridge curves are further divided into arcsegments between Type II landmarks for averaging. The complete set of average space curves represents the average surface.
 Internal tile curves have either u or v orientation (see
FIG. 36 ). Therefore each internal tile point belongs to two Bsplines. We compute the average u and v internal tile Bspline space curves separately. The average of these two points is then substituted back to internal tile vertices, in order to prepare a triangulated (graphical manifold) surface. Algorithmically, Bspline internal tile curve segments in the u and v direction may be represented as matrices: 
$\begin{array}{cc}{S}_{t}\ue8a0\left({C}_{u}\right)=\left[\begin{array}{cccc}{C}_{1,1}& {C}_{1,2}& \dots & {C}_{1,v}\\ {C}_{2,1}& {C}_{2,2}& \dots & {C}_{2,v}\\ \dots & \dots & \dots & \dots \\ {C}_{u,1}& {C}_{u,2}& \dots & {C}_{u,v}\end{array}\right],\text{}\ue89e{S}_{t}\ue8a0\left({C}_{v}\right)=\left[\begin{array}{cccc}{C}_{1,1}& {C}_{1,2}& \dots & {C}_{1,u}\\ {C}_{2,1}& {C}_{2,2}& \dots & {C}_{2,u}\\ \dots & \dots & \dots & \dots \\ {C}_{v,1}& {C}_{v,2}& \dots & {C}_{v,u}\end{array}\right]& (\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e49\end{array}$  The computed interior surface tile control points thus are the average of the above two matrices, S_{t}(C_{u}) and S_{t}(C_{v}):

$\begin{array}{cc}{S}_{t}\ue8a0\left(C\right)={S}_{t}\ue8a0\left({C}_{u}\right)+\hspace{1em}{{S}_{t}\ue8a0\left({C}_{v}\right)}^{t}=\left[\begin{array}{cccc}{C}_{1,1}& {C}_{1,2}& \dots & {C}_{1,v}\\ {C}_{2,1}& {C}_{2,2}& \dots & {C}_{2,v}\\ \dots & \dots & \dots & \dots \\ {C}_{u,1}& {C}_{u,2}& \dots & {C}_{u,v}\end{array}\right]+\left[\begin{array}{cccc}{C}_{1,1}& {C}_{1,2}& \dots & {C}_{1,u}\\ {C}_{2,1}& {C}_{2,2}& \dots & {C}_{2,u}\\ \dots & \dots & \dots & \dots \\ {C}_{v,1}& {C}_{v,2}& \dots & {C}_{v,u}\end{array}\right]& \left(\mathrm{EQ}\ue89e\phantom{\rule{0.8em}{0.8ex}}\ue89e50\right)\end{array}$  where t varies from 1 to the number of tiles in the whole sample surface.
 The SSA program was written in C in the Silicon Graphics Unix (IRIX) environment It was tested on a SGI Octane workstation (R10000 CPU, 256M byte RAM). The program interface integrates Procrustes superimposition, thin plate spline warp, and surface averaging methods with graphical manifold surface rendering operator feed back. User interaction occurs via X11/Motif protocols.
 To test the SSA program, we averaged soft tissue face surfaces (
FIG. 40A ) and skull surfaces (FIG. 40B ) from segmented from 3D CT volume images. These five 3D CT data sets (B2537, B2621, B3037, B3095, B3195) were provided by a recall of subjects. All subjects are above 60 years in age, female, have dental fillings, and often have large oral prostheses (dentures). The soft tissue face and skull surface segmentation of the volume images was performed in a separate program, SOFM. The ridge curvebased deformable template superimposition and surface parameterization was done in an another program, SASE.  We conducted a precision test of the SSA methods between two sessions by one operator. The operator introduces variation through initial seeding and subsequent fine tuning of the ridge and tiling curve template superimposition. The overall root mean square error between the surface tile points of two averaging sessions, of the boney skull surface average was 0.0 mm and for the soft tissue face surface average was 0.0 mm. This is complete agreement between sessions. This intraoperator reproducibility result (i.e., precision) provides evidence that the SSA method is reliable.
 We next compared the surface sampling used to produce average surfaces by the SSA program versus that of the toolkit using the same segmented, voxel data obtained from the SOFM program. We hypothesize that improved homology of tiling and internal tile curves in the SSA method will reduce the variance of the sample members about the average.
 We parameterized all 10 surfaces with both the SASE and the toolkit for the soft tissue face (
FIG. 41A ) and the boney skull (FIG. 41B ). Note that the toolkit sample and average surface images appear to bow along every ridge curve edge (e.g., orbits and nasal regions). Under the chin, we note that this problem has exaggerated a notch not seen in the SSA average. This bulging also results in smaller orbital and nasal apertures relative to the shape seen in the original segmented surface voxels. This and the smoother surface may be an artifact of splining a lesser number of points than in the SSA method.  We thin plate spline warped the average surface to the Type II landmark configuration of it's member surfaces. Then, we traced each fitted average surface tile point to the segmented surface image. Both averaging methods (i.e., toolkit and SSA) used surfaces extracted from the same segmented volume image, providing a common basis for comparison between the two methods. The computed square root of sum of squared differences are listed in Table XII. Note, the number of tile points in the toolkit surface extractions is well below that used in the SSA method. Given this difference, we observed an average of 6 mm distance for the toolkit averaging method for all points in the soft tissue face and skull surfaces, whereas the average SSA distance were 0.448 mm in the case of the soft tissue average and 0.419 mm in the case of the skull average. Table XIII presents these results tile by tile.
FIG. 42A displays color coded difference images of toolkit skull and soft tissue face surface averages with their source segmented voxel surfaces. SimilarlyFIG. 42B shows the same color coded difference images of the SSA skull and soft tissue face surface averages. The same range of difference is scaled from lowest difference in blue to largest difference in red in both images. Note that despite higher sampling density the internal tile points are as true as the tile borders in the SSA extractions.  In
FIG. 43A , we see the toolkit average surface prior to the final step of normal interpolation and averaging.FIG. 43B presents the same surfaces afterward.FIG. 43C presents the final SSA average surface; the SSA algorithm does not average surface normals. Note that seams are present but they less disturb the continuity of surface curvature between the tile surfaces. In both cases these are graphical rendering artifacts, not an indication of inaccuracy in surface parameterization or a nonshape preserving average.  One reason that the SSA average surface results better preserve surface curvature continuity, and reduce variance about the average, is the higher sampling density (
FIG. 44 ). The toolkit's graphical manifold is subsampled at four stages. Surface rendering of the segmented voxels introduces an error of ±1 to ±3 mm assuming a subsampling rate of 2×2×2.  Second, a wireframe is manually nudged into best position on this graphical manifold and then interpolated. This step increases the error at each point by ±1 to ±3 mm depending on the variable level of parametric smoothing that the program automatically applies. Third, the surface is sparsely encoded. This may eliminate some otherwise useful information from the patient surface image.
 Finally when generating the average, only the surface tile boundaries (i.e., ridge curve and geodesic lines) are truly averaged in the toolkit. The average inferior tile points are assigned, not found. Overall, the errors accumulated at each surface tile point vary from 3 to 6 mm. p It appears to us that the SSA method improves tiling and internal tile curve homology assignment. The SSA method better preserves overall shape of 3D surfaces during averaging because of (1) the improved homology assignment of internal tile points, and (2) the extension of the original space curve averaging method to the entire surface.
 The utility of average surface images for clinical diagnosis needs validation. Their use for boney prosthetic design is apparent. These methods were originally developed to quantify diagnostic morphometric differences of clinical populations, however we expect these data have applications at every stage of patient care, as well as other deformable model applications including animation.
 The invention has been described with reference to the preferred embodiment. Obviously, modifications and alterations will occur to others upon reading and understanding the preceding detailed description. It is intended that the invention be construed as including all such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (5)
 1. A method of fabricating a medical device, the method comprising:obtaining data regarding a shape of the medical device, wherein the shape is determined by:(a) rendering a computerbased threedimensional representation of a target tissue from computer readable image data of the target tissue wherein the target tissue comprises a portion with a defect and a portion without a defect;(b) mapping an external surface of the computergenerated threedimensional representation of the target tissue;(c) superimposing onto the mapped external surface a threedimensional template to span the defective portion by altering the shape of the threedimensional template to fit at least a portion of the mapped external surface; and(c) determining the shape of the medical device based on the shape of the altered threedimensional template that spans the defective region to obtain the data regarding the shape of the medical device; andfabricating the medical device at least partially based on using the obtained data regarding the shape of the medical device.
 2. The method of
claim 1 , wherein the image data is obtained from image slices of the target tissue.  3. The method of
claim 1 , wherein the image data is obtained as one or more voxels of the target tissue.  4. The method of
claim 1 , wherein the medical device is an implant to be implanted in a subject.  5. The method of
claim 1 , wherein fabricating the medical device comprises threedimensional printing at least a portion of the device.
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Families Citing this family (17)
Publication number  Priority date  Publication date  Assignee  Title 

US8781557B2 (en) *  19990811  20140715  Osteoplastics, Llc  Producing a three dimensional model of an implant 
US9208558B2 (en)  19990811  20151208  Osteoplastics Llc  Methods and systems for producing an implant 
US8603180B2 (en)  20060227  20131210  Biomet Manufacturing, Llc  Patientspecific acetabular alignment guides 
US9918740B2 (en)  20060227  20180320  Biomet Manufacturing, Llc  Backup surgical instrument system and method 
US9968376B2 (en)  20101129  20180515  Biomet Manufacturing, Llc  Patientspecific orthopedic instruments 
US7967868B2 (en)  20070417  20110628  Biomet Manufacturing Corp.  Patientmodified implant and associated method 
DE102011081987B4 (en) *  20110901  20140528  Tomtec Imaging Systems Gmbh  A method for generating a model of a surface of a cavity wall 
ES2558558T3 (en) *  20111216  20160205  Gestigon Gmbh  Method and device for estimating a position 
US9622820B2 (en) *  20120503  20170418  Siemens Product Lifecycle Management Software Inc.  Featuredriven rulebased framework for orthopedic surgical planning 
US9965893B2 (en) *  20130625  20180508  Google Llc.  Curvaturedriven normal interpolation for shading applications 
US9216084B2 (en)  20130809  20151222  Howmedica Osteonics Corp.  Patientspecific craniofacial implants 
US9076238B2 (en) *  20130821  20150707  Seiko Epson Corporation  Intelligent weighted blending for ultrasound image stitching 
WO2015081027A1 (en) *  20131129  20150604  The Johns Hopkins University  Patientspecific trackable cutting guides 
KR101718868B1 (en) *  20150921  20170322  한국과학기술연구원  Method for forming 3d mazillofacial model by automatically segmenting medical image, automatic image segmentation and model formation server performing the same, and storage mdeium storing the same 
US10078333B1 (en) *  20160417  20180918  X Development Llc  Efficient mapping of robot environment 
US20170319164A1 (en) *  20160509  20171109  Toshiba Medical Systems Corporation  Medical image diagnostic apparatus 
WO2018204611A1 (en)  20170503  20181108  The University Of Akron  Post3d printing functionalization of polymer scaffolds for enhanced bioactivity 
Family Cites Families (54)
Publication number  Priority date  Publication date  Assignee  Title 

DE3213434C1 (en)  19820410  19831027  Aldinger Guenther  Process for the production of individually designed prostheses or implants 
US4436684B1 (en)  19820603  19880531  
US4936862A (en)  19860530  19900626  Walker Peter S  Method of designing and manufacturing a human joint prosthesis 
DE3626549A1 (en)  19860806  19880211  Mecron Med Prod Gmbh  A process for the preparation of an endoprosthesis with individual adaptation 
US4976737A (en)  19880119  19901211  Research And Education Institute, Inc.  Bone reconstruction 
US5121333A (en)  19890609  19920609  Regents Of The University Of Minnesota  Method and apparatus for manipulating computerbased representations of objects of complex and unique geometry 
DE3933459A1 (en)  19891006  19910418  Karsten Dipl Ing Reumann  Biomedical implant production equipment  uses computer tomographic image to generate implant profile for component mfr. 
US5086401A (en) *  19900511  19920204  International Business Machines Corporation  Imagedirected robotic system for precise robotic surgery including redundant consistency checking 
GB9014057D0 (en)  19900625  19900815  Thackray C F Ltd  Prosthetic implant manufacture 
US5274565A (en)  19901003  19931228  Board Of Regents, The University Of Texas System  Process for making custom joint replacements 
US5156777A (en)  19910321  19921020  Kaye Alan H  Process for making a prosthetic implant 
GB9122843D0 (en)  19911028  19911211  Imperial College  Method and apparatus for image processing 
JP2713323B2 (en)  19920302  19980216  インターナショナル・ビジネス・マシーンズ・コーポレイション  Method and apparatus for displaying efficiently produced equality surface image data and surface isolines image data isosurface 
US5357429A (en)  19920402  19941018  Levy Richard A  Threedimensional model generation using multiple angle tomographic scan planes 
US5603318A (en)  19920421  19970218  University Of Utah Research Foundation  Apparatus and method for photogrammetric surgical localization 
DE4213599A1 (en)  19920424  19931028  Klaus Draenert  Prosthetic component and processes for their preparation 
US5370692A (en) *  19920814  19941206  Guild Associates, Inc.  Rapid, customized bone prosthesis 
US5360446A (en)  19921218  19941101  Zimmer, Inc.  Interactive prosthesis design system for implantable prosthesis 
DE69424733D1 (en)  19930420  20000706  Gen Electric  Graphic digital processing system and realtime video system for improving the representation of body structures during a surgical procedure. 
EP0722588B1 (en)  19930910  20030115  The University Of Queensland  Stereolithographic anatomical modelling process 
US5752962A (en)  19931115  19980519  D'urso; Paul S.  Surgical procedures 
DE4341367C1 (en)  19931204  19950614  Harald Dr Med Dr Med Eufinger  A process for the production of endoprostheses 
BE1008372A3 (en)  19940419  19960402  Materialise Nv  METHOD FOR MANUFACTURING A perfected MEDICAL MODEL BASED ON DIGITAL IMAGE INFORMATION OF A BODY. 
US5829444A (en)  19940915  19981103  Visualization Technology, Inc.  Position tracking and imaging system for use in medical applications 
DE4434539C2 (en)  19940927  19980604  Luis Dr Med Schuster  A process for producing an endoprosthesis as articular substitute for knee joints 
US5682886A (en) *  19951226  19971104  Musculographics Inc  Computerassisted surgical system 
RU2073490C1 (en)  19960207  19970220  Шалумов АрнольдСуруиль Зироевич  Method to prepare transplant to eliminate defects and deformations of supporting tissues 
US6126690A (en) *  19960703  20001003  The Trustees Of Columbia University In The City Of New York  Anatomically correct prosthesis and method and apparatus for manufacturing prosthesis 
JPH1075963A (en)  19960906  19980324  Nikon Corp  Method for designing dental prosthetic appliance model and medium recording program for executing the method 
GB2318058B (en)  19960925  20010321  Ninian Spenceley Peckitt  Improvements relating to prosthetic implants 
US5824085A (en)  19960930  19981020  Integrated Surgical Systems, Inc.  System and method for cavity generation for surgical planning and initial placement of a bone prosthesis 
DE19642247C1 (en)  19961012  19980115  Sebastian Meller  Prosthesis production system for prepared tooth 
US6205411B1 (en)  19970221  20010320  Carnegie Mellon University  Computerassisted surgery planner and intraoperative guidance system 
US5813984A (en)  19970307  19980929  University Radiologists, Inc.  Forensic skull and soft tissue database and online facial reconstruction of victims and age progession portrait rendering of missing children through utilization of advance diagnostic radiologic modalities 
GB2324470A (en)  19970424  19981028  Customflex Limited  Prosthetic implants 
US6298262B1 (en)  19980421  20011002  Neutar, Llc  Instrument guidance for stereotactic surgery 
US6327491B1 (en)  19980706  20011204  Neutar, Llc  Customized surgical fixture 
US6421048B1 (en) *  19980717  20020716  Sensable Technologies, Inc.  Systems and methods for interacting with virtual objects in a haptic virtual reality environment 
US7239908B1 (en)  19980914  20070703  The Board Of Trustees Of The Leland Stanford Junior University  Assessing the condition of a joint and devising treatment 
US6937696B1 (en)  19981023  20050830  Varian Medical Systems Technologies, Inc.  Method and system for predictive physiological gating 
US6424332B1 (en)  19990129  20020723  Hunter Innovations, Inc.  Image comparison apparatus and method 
US20070233272A1 (en) *  19990223  20071004  Boyce Todd M  Shaped loadbearing osteoimplant and methods of making same 
US6470207B1 (en)  19990323  20021022  Surgical Navigation Technologies, Inc.  Navigational guidance via computerassisted fluoroscopic imaging 
US6206927B1 (en)  19990402  20010327  Barry M. Fell  Surgically implantable knee prothesis 
US6415171B1 (en)  19990716  20020702  International Business Machines Corporation  System and method for fusing threedimensional shape data on distorted images without correcting for distortion 
WO2001010339A3 (en)  19990811  20010830  Univ Case Western Reserve  Method and apparatus for producing an implant 
US9208558B2 (en)  19990811  20151208  Osteoplastics Llc  Methods and systems for producing an implant 
WO2001033511A3 (en)  19991103  20011220  Univ Case Western Reserve  System and method for producing a threedimensional model 
US6772026B2 (en)  20000405  20040803  Therics, Inc.  System and method for rapidly customizing design, manufacture and/or selection of biomedical devices 
EP1372516B1 (en)  20010227  20090513  SMITH & NEPHEW, INC.  Surgical navigation systems for unicompartmental knee 
US7468075B2 (en)  20010525  20081223  Conformis, Inc.  Methods and compositions for articular repair 
WO2004110309A3 (en)  20030611  20050714  Univ Case Western Reserve  Computeraideddesign of skeletal implants 
CA2641241A1 (en)  20060206  20070816  Conformis, Inc.  Patient selectable joint arthroplasty devices and surgical tools 
CA2802119A1 (en)  20100611  20111215  Sunnybrook Health Sciences Center  Method of forming patientspecific implant 
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