US20170226142A1

US20170226142A1 - Factor D Inhibitors Useful for Treating Inflammatory Disorders

Info

Publication number: US20170226142A1
Application number: US15/494,926
Authority: US
Inventors: Jason Allan Wiles; Milind Deshpande
Original assignee: Achillion Pharmaceuticals Inc
Current assignee: Achillion Pharmaceuticals Inc
Priority date: 2014-02-25
Filing date: 2017-04-24
Publication date: 2017-08-10
Also published as: DK3110418T3; US9598446B2; CA2940645A1; JP2021193128A; MX2016011034A; US20150239893A1; EP3110419A4; BR112016019526B1; EP3110418A1; EP3623367B1; JP2017506269A; EP3110416A4; US10005802B2; EP3110806A4; US20160361329A1; EP3110806A1; US9796741B2; US20170298085A1; US10464956B2; KR20160116018A

Abstract

Compounds, methods of use, and processes for making inhibitors of complement factor D comprising Formula I, or a pharmaceutically acceptable salt or composition thereof are provided. The inhibitors described herein target factor D and inhibit or regulate the complement cascade at an early and essential point in the alternative complement pathway, and reduce factor D's ability to modulate the classical and lectin complement pathways. The inhibitors of factor D described herein are capable of reducing the excessive activation of complement, which has been linked to certain autoimmune, inflammatory, and neurodegenerative diseases, as well as ischemia-reperfusion injury and cancer.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 14/630,959, filed Feb. 25, 2015, which claims benefit of provisional U.S. Application No. 61/944,189 filed Feb. 25, 2014, provisional U.S. Application No. 62/022,916 filed Jul. 10, 2014, and provisional U.S. Application 62/046,783 filed Sep. 5, 2014. The entirety of each of these applications is hereby incorporated by reference for all purposes.

BACKGROUND

Complement Factor D is part of the alternative pathway of the complement cascade and plays an early and central role in activating the pathway. Complement Factor C3b is generated from complement component C3 by the C3 convertase enzyme, distinct forms of which are produced following activation of the alternative pathway and the classical and lectin pathways. Activation of the alternative complement pathway is initiated by spontaneous hydrolysis of a thioester bond within C3 to produce C3(H₂O), which associates with factor B to form the C3(H₂O)B complex. Complement Factor D acts to cleave factor B within the C3(H₂O)B complex to form Ba and Bb. The Bb fragment remains associated with C3(H₂O) to form the alternative pathway C3 convertase C3(H₂O)Bb. Additionally, C3b generated by any of the C3 convertases also associates with factor B to form C3bB, which factor D cleaves to generate the later stage alternative pathway C3 convertase C3bBb. This latter form of the alternative pathway C3 convertase may provide important downstream amplification within all three of the defined complement pathways, leading ultimately to the recruitment and assembly of additional factors in the complement cascade pathway, including the cleavage of C5 to C5a and C5b. C5b acts in the assembly of factors C6, C7, C8, and C9 into the membrane attack complex, which can destroy pathogenic cells by lysing the cell.
In some instances it is desirable to decrease the response of the alternative pathway. Activation of the alternative pathway of the complement cascade contributes to the production of C3a and C5a, both potent anaphylatoxins, which have roles in a number of inflammatory disorders. C3a and C5a are also formed by the classical pathway of the complement cascade. Regulation of the complement cascade is necessary to prevent damage to non-infected host cells. The balance between complement activation and inhibition is mediated by a series of complement regulatory factors, such as complement factor H and complement factor I.
Age-related macular degeneration (AMD) is a leading cause of vision loss in industrialized countries. There is evidence of the link between the complement cascade and macular degeneration. Individuals with mutations in the gene encoding complement factor H have a fivefold increased risk of macular degeneration and individuals with mutations in other complement factor genes also have an increased risk of AMD. Individuals with mutant factor H also have increased levels of C-reactive protein, a marker of inflammation. Without adequate functioning factor H the alternative pathway of the complement cascade is overly activated leading to cellular damage. Inhibition of the alternative pathway is thus desired.
Factor D is an attractive target for inhibition or regulation of the complement cascade due to its early and essential role in the alternative complement pathway, and its potential role in signal amplification within the classical and lectin complement pathways. Inhibition of Factor D effectively interrupts the pathway and attenuates the formation of the membrane attack complex. Compounds which act as Factor D inhibitors are needed for disruption of the complement cascade alternative pathway and treatment of disorders associated with defects in the complement cascade.

SUMMARY

The disclosure provides compounds of Formula I
and the pharmaceutically acceptable salts thereof. Within Formula I the variables, e.g, A, B, L, X¹, X², Q¹, Q², and Q³carry the following values.
Q¹is N(R¹) or C(R¹R^1′).
Q²is C(R²R^2′), C(R²R^2′)—C(R²R^2′), or C(R²R^2′)O.
Q³is N(R³), S, or C(R³R^3′).
(a) X¹and X²are independently N or CH, or (b) X¹and X²together are C═C.
R¹, R^1′, R², R^2′, R³, and R^3′ are independently chosen at each occurrence from (c) and (d):
(c) hydrogen, halogen, hydroxyl, nitro, cyano, amino, C₁-C₆alkyl, C₂-C₆alkenyl, C₁-C₆alkoxy, C₂-C₆alkynyl, C₂-C₆alkanoyl, C₁-C₆thioalkyl, hydroxyC₁-C₆alkyl, aminoC₁-C₆alkyl, —C₀-C₄alkylNR⁹R¹⁰, —C(O)OR⁹, —OC(O)R⁹, —NR⁹C(O)R¹⁰, —C(O)NR⁹R¹⁰, —OC(O)NR⁹R¹⁰, —NR⁹C(O)OR¹⁰, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy, where R⁹and R¹⁰are independently chosen at each occurrence from hydrogen, C₁-C₆alkyl, and (C₃-C₇cycloalkyl)C₀-C₄alkyl;
(d) —C₀-C₄alkyl(C₃-C₇cycloalkyl) and —O—C₀-C₄alkyl(C₃-C₇cycloalkyl).
Additionally any one of the following rings (e), (f), (g), (h), (i), or (j) may be present:
(e) R¹and R^1′ or R³and R^3′ may be taken together to form a 3- to 6-membered carbocyclic spiro ring or a 3- to 6-membered heterocyclic spiro ring containing 1 or 2 heteroatoms independently chosen from N, O, or S;
(f) R²and R^2′ may be taken together to form a 3- to 6-membered carbocyclic spiro ring,
(g) R²and R^2′ may be taken together to form a 3- to 6-membered heterocyclic spiro ring,
each of which spiro rings (e), (f), and (g) is unsubstituted or substituted with one or more halogen or methyl substituents;
(h) R¹and R²may be taken together to form a 3-membered carbocyclic ring;
(i) R¹and R²may be taken together to form a 4- to 6-membered carbocyclic ring or a 4- to 6-membered heterocyclic ring containing 1 or 2 heteroatoms independently chosen from N, O, and S.
(j) R²and R³, if bound to adjacent carbon atoms, may be taken together to form a 3- to 6-membered carbocyclic ring or a 3- to 6-membered heterocyclic ring; each of which ring (g), (h), and (i) may be unsubstituted or substituted with 1 or more substituents independently chosen from halogen, hydroxyl, cyano, —COOH, C₁-C₄alkyl, C₂-C₄alkenyl, C₁-C₄alkoxy, C₂-C₄alkanoyl, hydroxyC₁-C₄alkyl, (mono- and di-C₁-C₄alkylamino)C₀-C₄alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —O—C₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;
A is a heterocyclic group chosen from (k) and (l) where (k) is
and (l) is
X⁴is B(OH) and Y is CHR⁹; or X⁴is CHR⁹and Y is B(OH).
R⁴is (m) or (n):
(m) —CHO, —CONH₂, or C₂-C₆alkanoyl;
(n) hydrogen, —SO₂NH₂, —C(CH₂)F, —CH(CF₃)NH₂, C₁-C₆alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C(O)C₀-C₂alkyl(C₃-C₇cycloalkyl),
each of which R⁴other than hydrogen, —CHO, and —CONH₂, is unsubstituted or substituted with one or more of amino, imino, halogen, hydroxyl, cyano, cyanoimino, C₁-C₂alkyl, C₁-C₂alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
R⁵and R⁶are independently chosen from (o) and (p):
(o) —CHO, —C(O)NH₂, —C(O)NH(CH₃), or C₂-C₆alkanoyl;
(p) hydrogen, hydroxyl, halogen, cyano, nitro, —COOH, —SO₂NH₂, vinyl, C₁-C₆alkyl, C₂-C₆alkenyl, C₁-C₆alkoxy, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C(O)C₀-C₄alkyl(C₃-C₇cycloalkyl), —P(O)(OR⁹)₂, —OC(O)R⁹, —C(O)OR⁹, —C(O)N(CH₂CH₂R⁹)(R¹⁰), —NR⁹C(O)R¹⁰, phenyl, or 5- to 6-membered heteroaryl.
Each R⁵and R⁶other than hydrogen, hydroxyl, cyano, and —COOH is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, amino, imino, cyano, cyanoimino, C₁-C₂alkyl, C₁-C₄alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
R^6′ is hydrogen, halogen, hydroxyl, C₁-C₄alkyl, or C₁-C₄alkoxy; or R⁶and R^6′ may be taken together to form an oxo, vinyl, or imino group.
R⁷is hydrogen, C₁-C₆alkyl, or —C₀-C₄alkyl(C₃-C₇cycloalkyl.
R⁸and R^8′ are independently chosen from hydrogen, halogen, hydroxyl, C₁-C₆alkyl, C₁-C₆alkoxy, and (C₁-C₄alkylamino)C₀-C₂alkyl, or R⁸and R^8′ are taken together to form an oxo group.
R¹⁶is 0 or 1 or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
R¹⁹is hydrogen, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, —SO₂C₁-C₆alkyl, (mono- and di-C₁-C₆alkylamino)C₁-C₄alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), each of which R¹⁹other than hydrogen is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxyl, amino, —COOH, and —C(O)OC₁-C₄alkyl.
X¹¹is N or CR¹¹.
X¹²is N or CR¹².
X¹³is N or CR¹³.
X¹⁴is N or CR¹⁴.
X¹⁵is N or CR¹⁵.
No more than 2 of X¹¹, X¹², X¹³, X¹⁴, and X¹⁵are N.
R¹¹, R¹⁴, and R¹⁵are independently chosen at each occurrence from hydrogen, halogen, hydroxyl, nitro, cyano, —O(PO)(OR⁹)₂, —(PO)(OR⁹)₂, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C₀-C₄alkoxy(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
R¹²and R¹³are independently chosen from (q), (r), and (s):
(q) hydrogen, halogen, hydroxyl, nitro, cyano, amino, —COOH, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy,
(r) C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₂-C₆alkenyloxy, —C(O)OR⁹, C₁-C₆thioalkyl, —C₀-C₄alkylNR⁹R¹⁰, —C(O)NR⁹R¹⁰, —SO₂R⁹R¹⁰, —SO₂NR⁹R¹⁰, —OC(O)R⁹, and —C(NR⁹)NR⁹R¹⁰, each of which (r) is unsubstituted or substituted with one or more substituents independently selected from halogen, hydroxyl, nitro, cyano, amino, —COOH, —CONH₂C₁-C₂haloalkyl, and C₁-C₂haloalkoxy, and each of which (r) is also optionally substituted with one substituent chosen from phenyl and 4- to 7-membered heterocycle containing 1, 2, or 3 heteroatoms independently chosen from N, O, and S; which phenyl or 4- to 7-membered heterocycle is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl)(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;
(s) —C₂-C₆alkynyl, —C₂-C₆alkynylR²³, C₂-C₆alkanoyl, -JC₃-C₇cycloalkyl, —B(OH)₂, -JC(O)NR⁹R²³, -JOSO₂OR²¹, —C(O)(CH₂)_1-4S(O)R²¹, —O(CH₂)_1-4S(O)NR²¹NR²², -JOP(O)(OR²¹)(OR²²), -JP(O)(OR²¹)(OR²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JSP(O)(OR²¹)(OR²²), -JSP(O)(OR²¹)(R²²), -JSP(O)(R²¹)(R²²), -JNR⁹P(O)(NHR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(OR²²), -JC(S)R²¹, -JNR²¹SO₂R²², -JNR⁹S(O)NR²¹R²², -JNR⁹SO₂NR¹⁰R²², -JSO₂NR⁹COR²², —O(CH₂)_1-4SO₂NR²¹R²², -JSO₂NR⁹CONR²¹R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², -JOC(O)NR²⁴R²⁵, -JNR⁹C(O)OR¹⁰, -JNR⁹C(O)OR²³, -JNR²¹OC(O)R²², —(CH₂)_1-4C(O)NR²¹R²², -JC(O)R²⁴R²⁵, -JNR⁹C(O)R²¹, -JC(O)R²¹, -JNR⁹C(O)NR⁹R¹⁰, -JNR⁹C(O)NR²²R²³, -JNR⁹C(O)NR²⁴R²⁵, —CCR²¹, —(CH₂)_1-4OC(O)R²¹, -JC(O)OR²³, —C₂-C₄alkylR²³, —C₂-C₄alkenylR²³, —C₂-C₄alkynylR²³, and -Jparacyclophane.
J is independently chosen at each occurrence from a covalent bond, C₁-C₄alkylene, —OC₁-C₄alkylene, C₂-C₄alkenylene, and C₂-C₄alkynylene.
R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycle)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S.
R²³is independently chosen at each occurrence from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycle)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S.
R²⁴and R²⁵are taken together with the nitrogen to which they are attached to form a 4- to 7-membered monocyclic heterocycloalkyl group, or a 6- to 10-membered bicyclic heterocyclic group having fused, spiro, or bridged rings.
Each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
L is either (t), (u), or (v):
(t) is a group of the formula
where R¹⁷is hydrogen or C₁-C₆alkyl and R¹⁸and R^18′ are independently chosen from hydrogen, halogen, and methyl; and m is 0, 1, 2, or 3; and
(u) is a bond,
(v) or a group of the formula
B is a monocyclic or bicyclic carbocyclic or carbocyclic-oxy group or a monocyclic, bicyclic, or tricyclic heterocyclic group having 1, 2, 3, or 4 heteroatoms independently selected from N, O, and S and from 4 to 7 ring atoms per ring, or B is a C₂-C₆alkenyl or C₂-C₆alkynyl group.
Each of which B is unsubstituted or substituted with one or more substituents independently chosen from (w) and (x) and 0 or 1 substituents chosen from (y) and (z):
(w) halogen, hydroxyl, —COOH, cyano, C₁-C₆alkyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, —C₀-C₄alkylNR⁹R¹⁰, —SO₂R⁹, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;
(x) nitro, C₂-C₆alkenyl, C₂-C₆alkynyl, C₁-C₆thioalkyl, -JC₃-C₇cycloalkyl, —B(OH)₂, -JC(O)NR⁹R²³, -JOSO₂OR²¹, —C(O)(CH₂)_1-4S(O)R²¹, —O(CH₂)_1-4S(O)NR²¹R²¹R²², -JOP(O)(OR²¹)(OR²²), -JP(O)(OR²¹)(OR²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JSP(O)(OR²¹)(OR²²), -JSP(O)(OR²¹)(R²²), -JSP(O)(R²¹)(R²²), -JNR⁹P(O)(NHR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(OR²²), -JC(S)R²¹, -JNR²¹SO₂R²², -JNR⁹S(O)NR¹⁰R²², -JNR⁹SO₂NR¹⁰R²², -JSO₂NR⁹COR²², -JSO₂NR⁹CONR²¹R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², -JNR²¹C(O)OR²², -JNR²¹OC(O)R²², —(CH₂)_1-4C(O)NR²¹R²², -JC(O)R²⁴R²⁵, -JNR⁹C(O)R²¹, -JC(O)R²¹, -JNR⁹C(O)NR¹⁰R²², —CCR²¹, —(CH₂)_1-4OC(O)R²¹, and -JC(O)OR²³; each of which (x) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;
(y) naphthyl, naphthyloxy, indanyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl containing 1 or 2 heteroatoms chosen from N, O, and S, and bicyclic heterocycle containing 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and containing 4- to 7-ring atoms in each ring; each of which (y) is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —SO₂R⁹, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy; and
(z) tetrazolyl, (phenyl)C₀-C₂alkyl, (phenyl)C₁-C₂alkoxy, phenoxy, and 5- or 6-membered heteroaryl containing 1, 2, or 3 heteroatoms independently chosen from N, O, B, and S, each of which (z) is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —SO₂R⁹, —OSi(CH₃)₂C(CH₃)₃, —Si(CH₃)₂C(CH₃)₃, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
Either X²is nitrogen or at least one of (d), (e), (g), (i), (l), (n), (p), (s), (v), (x), and (y) is present. Pharmaceutical composition comprising a compound or salt of Formula I together with a pharmaceutically acceptable carrier are also disclosed.
Methods of treating or preventing disorders mediated by complement cascade Factor D, such as age-related macular degeneration and retinal degeneration, comprising administering a therapeutically effective amount of a compound or salt of Formula I to a patient in need of such treatment are also disclosed.

DETAILED DESCRIPTION

Terminology

Compounds are described using standard nomenclature. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. Unless clearly contraindicated by the context each compound name includes the free acid or free base form of the compound as well as all pharmaceutically acceptable salts of the compound.
The term “Formula I” encompasses all compounds that satisfy Formula I, including any enantiomers, racemates and stereoisomers, as well as all pharmaceutically acceptable salts of such compounds. “Formula I” includes all subgeneric groups of Formula I, such as Formula IA and Formula IB and also includes pharmaceutically acceptable salts of a compound of Formula I, unless clearly contraindicated by the context in which this phrase is used.
The terms “a” and “an” do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced item. The term “or” means “and/or”. The open-ended transitional phrase “comprising” encompasses the intermediate transitional phrase “consisting essentially of” and the close-ended phrase “consisting of.” Claims reciting one of these three transitional phrases, or with an alternate transitional phrase such as “containing” or “including” can be written with any other transitional phrase unless clearly precluded by the context or art. Recitation of ranges of values are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. The endpoints of all ranges are included within the range and independently combinable. All methods described herein can be performed in a suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”), is intended merely to better illustrate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention as used herein. Unless defined otherwise, technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs.
Compounds of Formula I include all compounds of Formula I having isotopic substitutions at any position. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example, and without limitation, isotopes of hydrogen include tritium and deuterium and isotopes of carbon include ¹¹C, ¹³C, and ¹⁴C. While the compounds of Formula I require a moderate or high level of deuteration (substitution of a hydrogen with deuterium) at identified positions, Formula I includes embodiments in which other positions are isotopically enriched.
An “active agent” means a compound (including a compound disclosed herein), element, or mixture that when administered to a patient, alone or in combination with another compound, element, or mixture, confers, directly or indirectly, a physiological effect on the patient. The indirect physiological effect may occur via a metabolite or other indirect mechanism.
A dash (“-”) that is not between two letters or symbols is used to indicate a point of attachment for a substituent. For example, —(C═O)NH₂is attached through carbon of the keto (C═O) group.
“Alkyl” is a branched or straight chain saturated aliphatic hydrocarbon group, having the specified number of carbon atoms, generally from 1 to about 12 carbon atoms. The term C₁-C₆alkyl as used herein indicates an alkyl group having from 1, 2, 3, 4, 5, or 6 carbon atoms. Other embodiments include alkyl groups having from 1 to 8 carbon atoms, 1 to 4 carbon atoms or 1 or 2 carbon atoms, e.g. C₁-C₈alkyl, C₁-C₄alkyl, and C₁-C₂alkyl. When C₀-C_nalkyl is used herein in conjunction with another group, for example, (C₃-C₇cycloalkyl)C₀-C₄alkyl, or —C₀-C₄alkyl(C₃-C₇cycloalkyl), the indicated group, in this case cycloalkyl, is either directly bound by a single covalent bond (C₀alkyl), or attached by an alkyl chain having the specified number of carbon atoms, in this case 1, 2, 3, or 4 carbon atoms. Alkyls can also be attached via other groups such as heteroatoms as in —O—C₀-C₄alkyl(C₃-C₇cycloalkyl). Examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, 3-methylbutyl, t-butyl, n-pentyl, and sec-pentyl.
“Alkenyl” is a branched or straight chain aliphatic hydrocarbon group having one or more carbon-carbon double bonds that may occur at any stable point along the chain, having the specified number of carbon atoms. Examples of alkenyl include, but are not limited to, ethenyl and propenyl.
“Alkynyl” is a branched or straight chain aliphatic hydrocarbon group having one or more double carbon-carbon triple bonds that may occur at any stable point along the chain, having the specified number of carbon atoms.
“Alkylene” is a bivalent saturated hydrocarbon. Alkylenes include groups having 1 to 8 carbon atoms, 1 to 6 carbon atoms, or the indicated number of carbon atoms, for example C₁-C₄alkylene.
“Alkenylene” is a bivalent hydrocarbon having at least one carbon-carbon double bond. Alkenylenes include groups having 2 to 8 carbon atoms, 2 to 6 carbon atoms, or the indicated number of carbon atoms, for example C₂-C₄alkenylene.
“Alkynylene” is a bivalent hydrocarbon having at least one carbon-carbon triple bond. Alkynylenes include groups having 2 to 8 carbon atoms, 2 to 6 carbon atoms, or the indicated number of carbon atoms, for example C₂-C₄alkenylene.
“Alkoxy” is an alkyl group as defined above with the indicated number of carbon atoms covalently bound to the group it substitutes by an oxygen bridge (—O—). Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, 2-butoxy, t-butoxy, n-pentoxy, 2-pentoxy, 3-pentoxy, isopentoxy, neopentoxy, n-hexoxy, 2-hexoxy, 3-hexoxy, and 3-methylpentoxy. Similarly an “Alkylthio” or a “thioalkyl” group is an alkyl group as defined above with the indicated number of carbon atoms covalently bound to the group it substitutes by a sulfur bridge (—S—).
“Alkenyloxy” is an alkenyl group as defined above with the indicated number of carbon atoms covalently bound to the group it substitutes by an oxygen bridge (—O—).
“Alkanoyl” is an alkyl group as defined above with the indicated number of carbon atoms covalently bound to the group is substitutes through a carbonyl (C═O) bridge. The carbonyl carbon is included in the number of carbons, that is C₂alkanoyl is a CH₃(C═O)— group.
“Alkylester” is an alkyl group as defined herein covalently bound to the group it substitutes by an ester linkage. The ester linkage may be in either orientation, e.g., a group of the formula —O(C═O)alkyl or a group of the formula —(C═O)Oalkyl.
“Carbocyclic group” is a saturated, unsaturated, or partially unsaturated (e.g. aromatic) group containing all carbon ring atoms. A carbocyclic group typically contains 1 ring of 3 to 7 carbon atoms or 2 fused rings each containing 3 to 7 carbon atoms.
“Carbocyclic ring” is a saturated, unsaturated, or partially unsaturated (e.g. aromatic) ring containing all carbon ring atoms. A carbocyclic ring typically contains 1 ring of 3 to 7 carbon atoms or a “carbocyclic group” may contain 1 carbocyclic ring or 2 fused carbocyclic rings each containing 3 to 7 carbon atoms. Examples of carbocyclic rings include phenyl, cyclohexenyl, cyclohexyl, and cyclopropyl rings.
“Carbocyclic-oxy group” is a monocyclic carbocyclic ring or a mono- or bi-cyclic carbocyclic group as defined above attached to the group it substitutes via an oxygen, —O—, linker.
“Cycloalkyl” is a saturated hydrocarbon ring group, having the specified number of carbon atoms. Monocyclic cycloalkyl groups typically have from 3 to about 8 carbon ring atoms or from 3 to 7 (3, 4, 5, 6, or 7) carbon ring atoms. Cycloalkyl substituents may be pendant from a substituted nitrogen or carbon atom, or a substituted carbon atom that may have two substituents may have a cycloalkyl group, which is attached as a spiro group. Examples of cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.
“Haloalkyl” indicates both branched and straight-chain alkyl groups having the specified number of carbon atoms, substituted with 1 or more halogen atoms, up to the maximum allowable number of halogen atoms. Examples of haloalkyl include, but are not limited to, trifluoromethyl, difluoromethyl, 2-fluoroethyl, and penta-fluoroethyl.
“Haloalkoxy” indicates a haloalkyl group as defined herein attached through an oxygen bridge (oxygen of an alcohol radical).
“Hydroxyalkyl” is an alkyl group as previously described, substituted with at least one hydroxyl subsitutuent.
“Aminoalkyl” is an alkyl group as previously described, substituted with at least one amino subsitutuent. “Halo” or “halogen” indicates any of fluoro, chloro, bromo, and iodo.
“Aryl” indicates aromatic groups containing only carbon in the aromatic ring or rings. Typical aryl groups contain 1 to 3 separate, fused, or pendant rings and from 6 to about 18 ring atoms, without heteroatoms as ring members. When indicated, such aryl groups may be further substituted with carbon or non-carbon atoms or groups. Such substitution may include fusion to a 5 to 7-membered saturated cyclic group that optionally contains 1 or 2 heteroatoms independently chosen from N, O, and S, to form, for example, a 3,4-methylenedioxy-phenyl group. Aryl groups include, for example, phenyl, naphthyl, including 1-naphthyl and 2-naphthyl, and bi-phenyl.
A “Heterocyclic ring” is a saturated, unsaturated, or partially unsaturated (e.g. aromatic) ring containing 1 to 4 ring heteroatoms independently chosen from N, O, and S, or if indicated, N, O, S, and B, with remaining ring atoms being carbon. A“heterocyclic group” may contain 1 heterocyclic ring 1 ring of 3 to 7 ring atoms or 2 fused rings each containing 3 to 7 ring atoms with at least one ring being a heterocyclic ring.
“Heterocyclicoxy group” is a monocyclic heterocyclic ring or a bicyclic heterocyclic group as described previously linked to the group it substitutes via an oxygen, —O—, linker.
“Heteroaryl” indicates a stable monocyclic aromatic ring having the indicated number of ring atoms which contains from 1 to 3, or in some embodiments from 1 to 2, heteroatoms chosen from N, O, and S, with remaining ring atoms being carbon, or a stable bicyclic or tricyclic system containing at least one 5- to 7-membered aromatic ring which contains from 1 to 3, or in some embodiments from 1 to 2, heteroatoms chosen from N, O, and S, with remaining ring atoms being carbon. Monocyclic heteroaryl groups typically have from 5 to 7 ring atoms. In some embodiments bicyclic heteroaryl groups are 9- to 10-membered heteroaryl groups, that is, groups containing 9 or 10 ring atoms in which one 5- to 7-member aromatic ring is fused to a second aromatic or non-aromatic ring. When the total number of S and O atoms in the heteroaryl group exceeds 1, these heteroatoms are not adjacent to one another. It is preferred that the total number of S and O atoms in the heteroaryl group is not more than 2. It is particularly preferred that the total number of S and O atoms in the aromatic heterocycle is not more than 1. Examples of heteroaryl groups include, but are not limited to, oxazolyl, pyranyl, pyrazinyl, pyrazolopyrimidinyl, pyrazolyl, pyridizinyl, pyridyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienylpyrazolyl, thiophenyl, triazolyl, benzo[d]oxazolyl, benzofuranyl, benzothiazolyl, benzothiophenyl, benzoxadiazolyl, dihydrobenzodioxynyl, furanyl, imidazolyl, indolyl, and isoxazolyl. “Heteroaryloxy” is a heteroaryl group as described bound to the group it substituted via an oxygen bridge.
“Heterocycloalkyl” is a saturated ring group, having 1, 2, 3, or 4 heteroatoms independently chosen from N, S, and O, with remaining ring atoms being carbon. Monocyclic heterocycloalkyl groups typically have from 3 to about 8 ring atoms or from 4 to 6 ring atoms. Examples of heterocycloalkyl groups include morpholinyl, piperazinyl, piperidinyl, and pyrrolinyl.
The term “mono- and/or di-alkylamino” indicates secondary or tertiary alkyl amino groups, wherein the alkyl groups are independently chosen alkyl groups, as defined herein, having the indicated number of carbon atoms. The point of attachment of the alkylamino group is on the nitrogen. Examples of mono- and di-alkylamino groups include ethylamino, dimethylamino, and methyl-propyl-amino.
The term “substituted”, as used herein, means that any one or more hydrogens on the designated atom or group is replaced with a selection from the indicated group, provided that the designated atom's normal valence is not exceeded. When the substituent is oxo (i.e., ═O) then 2 hydrogens on the atom are replaced. When an oxo group substitutes aromatic moieties, the corresponding partially unsaturated ring replaces the aromatic ring. For example a pyridyl group substituted by oxo is a pyridone. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds or useful synthetic intermediates. A stable compound or stable structure is meant to imply a compound that is sufficiently robust to survive isolation from a reaction mixture, and subsequent formulation into an effective therapeutic agent. Unless otherwise specified substituents are named into the core structure. For example, it is to be understood that when aminoalkyl is listed as a possible substituent the point of attachment of this substituent to the core structure is in the alkyl portion.
Suitable groups that may be present on a “substituted” or “optionally substituted” position include, but are not limited to, e.g., halogen; cyano; hydroxyl; nitro; azido; alkanoyl (such as a C₂-C₆alkanoyl group); carboxamide; alkyl groups (including cycloalkyl groups) having 1 to about 8 carbon atoms, or 1 to about 6 carbon atoms; alkenyl and alkynyl groups including groups having one or more unsaturated linkages and from 2 to about 8, or 2 to about 6 carbon atoms; alkoxy groups having one or more oxygen linkages and from 1 to about 8, or from 1 to about 6 carbon atoms; aryloxy such as phenoxy; alkylthio groups including those having one or more thioether linkages and from 1 to about 8 carbon atoms, or from 1 to about 6 carbon atoms; alkylsulfinyl groups including those having one or more sulfinyl linkages and from 1 to about 8 carbon atoms, or from 1 to about 6 carbon atoms; alkylsulfonyl groups including those having one or more sulfonyl linkages and from 1 to about 8 carbon atoms, or from 1 to about 6 carbon atoms; aminoalkyl groups including groups having one or more N atoms and from 1 to about 8, or from 1 to about 6 carbon atoms; aryl having 6 or more carbons and one or more rings, (e.g., phenyl, biphenyl, naphthyl, or the like, each ring either substituted or unsubstituted aromatic); arylalkyl having 1 to 3 separate or fused rings and from 6 to about 18 ring carbon atoms, with benzyl being an exemplary arylalkyl group; arylalkoxy having 1 to 3 separate or fused rings and from 6 to about 18 ring carbon atoms, with benzyloxy being an exemplary arylalkoxy group; or a saturated, unsaturated, or aromatic heterocyclic group having 1 to 3 separate or fused rings with 3 to about 8 members per ring and one or more N, O or S atoms, e.g. coumarinyl, quinolinyl, isoquinolinyl, quinazolinyl, pyridyl, pyrazinyl, pyrimidinyl, furanyl, pyrrolyl, thienyl, thiazolyl, triazinyl, oxazolyl, isoxazolyl, imidazolyl, indolyl, benzofuranyl, benzothiazolyl, tetrahydrofuranyl, tetrahydropyranyl, piperidinyl, morpholinyl, piperazinyl, and pyrrolidinyl. Such heterocyclic groups may be further substituted, e.g. with hydroxy, alkyl, alkoxy, halogen and amino. In certain embodiments “optionally substituted” includes one or more substituents independently chosen from halogen, hydroxyl, amino, cyano, —CHO, —COOH, —CONH₂, C₁-C₆alkyl, C₂-C₆alkenyl, C₁-C₆alkoxy, C₂-C₆alkanoyl, C₁-C₆alkylester, (mono- and di-C₁-C₆alkylamino)C₀-C₂alkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
A “dosage form” means a unit of administration of an active agent. Examples of dosage forms include tablets, capsules, injections, suspensions, liquids, emulsions, creams, ointments, suppositories, inhalable forms, transdermal forms, and the like.
“Pharmaceutical compositions” are compositions comprising at least one active agent, such as a compound or salt of Formula I, and at least one other substance, such as a carrier. Pharmaceutical compositions optional contain one or more additional active agents. When specified, pharmaceutical compositions meet the U.S. FDA's GMP (good manufacturing practice) standards for human or non-human drugs. “Pharmaceutical combinations” are combinations of at least two active agents which may be combined in a single dosage form or provided together in separate dosage forms with instructions that the active agents are to be used together to treat a disorder, such as hepatitis C.
“Pharmaceutically acceptable salts” includes derivatives of the disclosed compounds in which the parent compound is modified by making inorganic and organic, non-toxic, acid or base addition salts thereof. The salts of the present compounds can be synthesized from a parent compound that contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting free acid forms of these compounds with a stoichiometric amount of the appropriate base (such as Na, Ca, Mg, or K hydroxide, carbonate, bicarbonate, or the like), or by reacting free base forms of these compounds with a stoichiometric amount of the appropriate acid. Such reactions are typically carried out in water or in an organic solvent, or in a mixture of the two. Generally, non-aqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred, where practicable. Salts of the present compounds further include solvates of the compounds and of the compound salts.
Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts include the conventional non-toxic salts and the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, conventional non-toxic acid salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, mesylic, esylic, besylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, HOOC—(CH₂)_n—COOH where n is 0-4, and the like. Lists of additional suitable salts may be found, e.g., in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., p. 1418 (1985).
The term “carrier” applied to pharmaceutical compositions/combinations of the invention refers to a diluent, excipient, or vehicle with which an active compound is provided.
A “pharmaceutically acceptable excipient” means an excipient that is useful in preparing a pharmaceutical composition/combination that is generally safe, non-toxic and neither biologically nor otherwise undesirable, and includes an excipient that is acceptable for veterinary use as well as human pharmaceutical use. A “pharmaceutically acceptable excipient” as used in the present application includes both one and more than one such excipient.
A “patient” is a human or non-human animal in need of medical treatment. Medical treatment can include treatment of an existing condition, such as a disease or disorder, prophylactic or preventative treatment, or diagnostic treatment. In some embodiments the patient is a human patient.
“Providing” means giving, administering, selling, distributing, transferring (for profit or not), manufacturing, compounding, or dispensing.
“Providing a compound of Formula I with at least one additional active agent” means the compound of Formula I and the additional active agent(s) are provided simultaneously in a single dosage form, provided concomitantly in separate dosage forms, or provided in separate dosage forms for administration separated by some amount of time that is within the time in which both the compound of Formula I and the at least one additional active agent are within the blood stream of a patient. In certain embodiments the compound of Formula I and the additional active agent need not be prescribed for a patient by the same medical care worker. In certain embodiments the additional active agent or agents need not require a prescription. Administration of the compound of Formula I or the at least one additional active agent can occur via any appropriate route, for example, oral tablets, oral capsules, oral liquids, inhalation, injection, suppositories or topical contact.
“Treatment,” as used herein includes providing a compound of Formula I, either as the only active agent or together with at least one additional active agent sufficient to: (a) prevent a disease or a symptom of a disease from occurring in a patient who may be predisposed to the disease but has not yet been diagnosed as having it (e.g. including diseases that may be associated with or caused by a primary disease (as in macular degeneration that can result in the context of factor D activation); (b) inhibiting the disease, i.e. arresting its development; and (c) relieving the disease, i.e., causing regression of the disease. “Treating” and “treatment” also means providing a therapeutically effective amount of a compound of Formula I, as the only active agent or together with at least one additional active agent to a patient having or susceptible to a condition mediated by complement factor D.
A “therapeutically effective amount” of a pharmaceutical composition/combination of this invention means an amount effective, when administered to a patient, to provide a therapeutic benefit such as an amelioration of symptoms, e.g., an amount effective to decrease the symptoms of a macular degeneration. A therapeutically effective amount is also an amount sufficient to prevent a significant increase or significantly reduce the detectable level of complement Factor D in the patient's blood, serum, or tissues.

Chemical Description

In addition to compounds of Formula I shown in the SUMMARY section the disclosure also include compounds in which the variables, e.g., A, B, L, R¹-R^3′, and L carry the following definitions. The disclosure includes all combinations of these definitions so long as a stable compound results.
For example the disclosure includes compounds and salts of Formula II, III, IV, V, VI, VII, VIII, IX, X, XI, XII, XIII, XIV, XV, XVI, XVII, XVIII, XIX, XX, XXI, and XXII which are with in the scope of Formula I. The variables shown in Formula II-XXIV carry the definitions set forth in the SUMMARY section for Formula I or any of the definitions set forth in this disclosure.
Additionally, the disclosure includes compounds and salts of Formula I and any of its subformulae (II-XXIV) in which at least one of the following conditions is met.
R¹, R^1′, R^2′, R³, and R^3′, if present, are all hydrogen; and R²is fluoro.
R¹, R^1′, R^2′, and R^3′, if present, are all hydrogen; and R²is fluoro and R³is —C₀-C₄alkyl(C₃-C₇cycloalkyl) or —O—C₀-C₄alkyl(C₃-C₇cycloalkyl.
R¹and R²are taken together to form a 3- to 6-membered cycloalkyl group, and R^1′, R^2′, R³, and R^3′, where present, are all hydrogen.
R¹, R^1′, R³, and R^3′, if present, are all hydrogen, and R²and R^2′ are taken together to form a 5- or 6-membered heterocycloalkyl group having 1 or 2 oxygen atoms.
-L-B— is
where
R²⁶and R²⁷are independently chosen from hydrogen, halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C₀-C₄alkoxy(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, C₁-C₂haloalkoxy, and C₁-C₂haloalkylthio.
(f) -L-B— is
wherein
R¹⁸and R^18′ are independently chosen from hydrogen, halogen, and methyl; and m is 0 or 1; and
R²⁶, R²⁷, and R²⁸are independently chosen from hydrogen, halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, (C₃-C₇cycloalkyl)C₀-C₄alkyl, and —C₀-C₄alkoxy(C₃-C₇cycloalkyl); each of which R²⁶, R²⁷, and R²⁸other than hydrogen, halogen, hydroxyl, nitro, cyano, is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, amino, C₁-C₂alkoxy, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy; and
R²⁹is hydrogen, C₁-C₂alkyl, C₁-C₂haloalkyl or —Si(CH₃)₂C(CH₃)₃.
(g) R⁸and R^8′ are independently hydrogen or methyl.
(h) R⁸and R^8′ are hydrogen.
(i) R⁷is hydrogen or methyl.
(j) R⁷is hydrogen.
(k) One of R¹²and R¹³is chosen from hydrogen, halogen, hydroxyl, amino, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), —OC₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
(l) R¹, R^1′, R², and R^3′ are all hydrogen;
R²is fluoro and R³is hydrogen, —C₀-C₄alkyl(C₃-C₇cycloalkyl), or —O—C₀-C₄alkyl(C₃-C₇cycloalkyl);
R⁵is hydrogen, halogen, or C₁-C₂alkyl;
R¹¹, R¹³R¹⁴, and R¹⁵, if present, are independently chosen at each occurrence from hydrogen, halogen, hydroxyl, amino, C₁-C₄alkyl, C₁-C₄alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₂alkylamino), trifluoromethyl, and trifluoromethoxy;
X¹²is CR¹²; and
R¹²is -JNR⁹C(O)OR, -JNR⁹C(O)OR²³, -JOC(O)NR²¹R²², -JOC(O)NR²⁴R²⁵, -JNR⁹C(O)NR¹⁰R²³, or -JNR⁹C(O)NR²⁴R²⁵.
(m) J is a bond.
(n) One of R¹²and R¹³is selected from
where p is 0, 1, 2, 3, or 4.
(o) The disclosure includes compounds and salts for Formula VII
wherein:
R¹, R², R^2′, and R³are independently chosen from hydrogen, halogen, C₁-C₄alkyl, C₁-C₄alkoxy, —C₀-C₂alkylNR⁹R¹⁰, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —O—C₀-C₄alkyl(C₃-C₇cycloalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;
R⁸and R^8′ are independently chosen from hydrogen, halogen, and methyl;
R⁵is hydrogen, hydroxyl, cyano, —COOH, C₁-C₆alkyl, C₁-C₆alkoxy, C₂-C₆alkanoyl —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C(O)C₀-C₄alkyl(C₃-C₇cycloalkyl, C₁-C₂haloalkyl, or C₁-C₂haloalkoxy;
R⁶is —C(O)CH₃, —C(O)NH₂, —C(O)CF₃, —C(O)(cyclopropyl), or -ethyl(cyanoimino); and
R¹¹and R¹⁴are independently chosen from hydrogen, halogen, hydroxyl, amino, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), —OC₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
(p) B is selected from
where R²⁷is hydrogen, methyl, or trifluoromethyl; R²⁸is hydrogen or halogen; and R²⁹is hydrogen, methyl, trifluoromethyl, or —Si(CH₃)₂C(CH₃)₃.
(q) B is phenyl, pyridyl, or indanyl each of which is unsubstituted or substituted with one or more substituents independently chosen from hydrogen, halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, (C₃-C₇cycloalkyl)C₀-C₄alkyl, —C₀-C₄alkoxy(C₃-C₇cycloalkyl), (phenyl)C₀-C₂alkyl, (pyridyl)C₀-C₂alkyl; each of which substituents other than hydrogen, halogen, hydroxyl, nitro, cyano, is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, amino, C₁-C₂alkyl, C₁-C₂alkoxy, —OSi(CH₃)₂C(CH₃)₃, —Si(CH₃)₂C(CH₃)₃, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
(r) B is phenyl or pyridyl substituted with 1, 2, or 3 substituents chosen from chloro, bromo, hydroxyl, —SCF₃, C₁-C₂alkyl, C₁-C₂alkoxy, trifluoromethyl, and trifluoromethoxy.
(s) A is a group of the formula
(t) -L-B is a bond and indanyl group of the formula
This disclosure further includes embodiments in which m is 0 or 1;
R²is halogen, R^2′ is hydrogen or halogen, and R³is hydrogen, halogen, —C₀-C₄alkyl(C₃-C₇cycloalkyl), or —O—C₀-C₄alkyl(C₃-C₇cycloalkyl;
R⁶is —C(O)C₁-C₄alkyl, —C(O)NH₂, —C(O)CF₃, —C(O)(C₃-C₇cycloalkyl), or -ethyl(cyanoimino);
one of R¹²and R¹³is selected from hydrogen, halogen, C₁-C₄alkyl, C₁-C₄alkoxy, trifluoromethyl, and trifluoromethoxy; the other of R¹²and R¹³a is chosen from (s),
where (s) is C₂-C₆alkynyl, —C₂-C₆alkynylR²³, C₂-C₆alkanoyl, -JC₃-C₇cycloalkyl, —B(OH)₂, -JC(O)NR⁹R²³, -JOSO₂OR²¹, —C(O)(CH₂)_1-4S(O)R²¹, —O(CH₂)_1-4S(O)NR²¹NR²², -JOP(O)(OR²¹)(OR²²), -JP(O)(OR²¹)(OR²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JSP(O)(OR²¹)(OR²²), -JSP(O)(OR²¹)(R²²), -JSP(O)(R²¹)(R²²), -JNR⁹P(O)(NHR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(OR²²), -JC(S)R²¹, -JNR²¹SO₂R²², -JNR⁹S(O)NR¹⁰R²², JNR⁹SO₂NR¹⁰R²², -JSO₂NR⁹COR²², —O(CH₂)_1-4SO₂NR²¹R²², -JSO₂NR⁹CONR²¹R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NCN, -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², -JOC(O)NR²⁴R²⁵, -JNR⁹C(O)OR¹⁰, -JNR⁹C(O)OR²³, -JNR²¹OC(O)R²², —(CH₂)_1-4C(O)NR²¹R²², -JNR⁹C(O)R²¹, -JC(O)R²¹, -JNR⁹C(O)NR⁹R¹⁰, -JNR⁹C(O)NR¹⁰R²³, -JNR⁹C(O)NR²⁴R²⁵, —CCR²¹, —(CH₂)_1-4OC(O)R²¹, -JC(O)OR²³, —C₂-C₄alkylR²³, and -Jparacyclophane; where J is independently chosen at each occurrence and is a covalent bond, C₁-C₄alkylene, C₂-C₄alkenylene, or C₂-C₄alkynylene;
R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S;
R²³is independently chosen at each occurrence from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycle)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S;
R²⁴and R²⁵are taken together with the nitrogen to which they are attached to form a 4- to 7-membered monocyclic heterocycloalkyl group, or a 6- to 10-membered bicyclic heterocycloalkyl group having fused, spiro, or bridged rings; each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
(r) This disclosure includes compounds and salts in which one of R¹²and R¹³is hydrogen, hydroxyl, halogen, methyl, or methoxy; and the other of
R¹²and R¹³is independently is chosen from (s), where (s) is C₂-C₆alkynyl, —C₂-C₆alkynylR²³, C₂-C₆alkanoyl, -JC₃-C₇cycloalkyl, -JC(O)NR⁹R²³, —C(O)(CH₂)_1-4S(O)R²¹, -JP(O)(OR²¹)(OR²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JNR²¹SO₂R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NCN, -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², -JOC(O)NR²⁴R²⁵, -JNR⁹C(O)OR¹⁰, -JNR⁹C(O)OR²³, -JNR²¹OC(O)R²², -JC(O)R²¹, -JNR⁹C(O)NR⁹R¹⁰, -JNR⁹C(O)NR¹⁰R²³, -JNR⁹C(O)NR²⁴R²⁵, and -Jparacyclophane; where J is independently chosen at each occurrence and is a covalent bond, C₁-C₄alkylene, C₂-C₄alkenylene, or C₂-C₄alkynylene;
R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl, (pyrrolidinyl)C₀-C₄alkyl, ((morpholinyl)C₀-C₄alkyl, (thiomorpholinyl)C₀-C₄alkyl, (piperidinyl)C₀-C₄alkyl, (piperazinyl)C₀-C₄alkyl, (tetrahydrofuranyl)C₀-C₄alkyl, pyrazolyl)C₀-C₄alkyl, (thiazolyl)C₀-C₄alkyl, (triazolyl)C₀-C₄alkyl, (tetrazolyl)C₀-C₄alkyl, (imidazolyl)C₀-C₄alkyl, (oxazolyl)C₀-C₄alkyl, (furanyl)C₀-C₄alkyl, (pyridinyl)C₀-C₄alkyl, (pyrimidinyl)C₀-C₄alkyl, (pyrazinyl)C₀-C₄alkyl, (pyridizinyl)C₀-C₄alkyl, and (tetrahydropyridinyl)C₀-C₄alkyl;
R²³is independently chosen at each occurrence from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (pyrrolidinyl)C₀-C₄alkyl, (morpholinyl)C₀-C₄alkyl, (thiomorpholinyl)C₀-C₄alkyl, (piperidinyl)C₀-C₄alkyl, (piperazinyl)C₀-C₄alkyl, (tetrahydrofuranyl)C₀-C₄alkyl, (pyrazolyl)C₀-C₄alkyl, (thiazolyl)C₀-C₄alkyl, (triazolyl)C₀-C₄alkyl, (tetrazolyl)C₀-C₄alkyl, (imidazolyl)C₀-C₄alkyl, (oxazolyl)C₀-C₄alkyl, (furanyl)C₀-C₄alkyl, (pyridinyl)C₀-C₄alkyl, (pyrimidinyl)C₀-C₄alkyl, (pyrazinyl)C₀-C₄alkyl, (pyridizinyl)C₀-C₄alkyl, and (tetrahydropyridinyl)C₀-C₄alkyl;
R²⁴and R²⁵are taken together with the nitrogen to which they are attached to form a pyrrolidinyl, piperazinyl, piperidinyl, or morpholinyl group, each of which is optionally bridged with a methylene or ethylene group or spiro to a C₃-C₅cycloalkyl group;
each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
This disclosure includes compounds and salts in which one of R¹²and R¹³is hydrogen, hydroxyl, halogen, methyl, or methoxy; and the other of R¹²and R¹³is chosen from (s), where (s) is -JP(O)(OR²¹)(OR²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², or -JP(O)R²¹R²²;
where J is independently chosen at each occurrence and is a covalent bond, C₁-C₄alkylene, C₂-C₄alkenylene, or C₂-C₄alkynylene;
R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, and —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl;
each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
This disclosure includes compounds and salts in which one of R¹²and R¹³is hydrogen, hydroxyl, halogen, methyl, or methoxy; and the other of R¹²and R¹³is —C₂-C₆alkynylR²³; where
R²³is from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (pyrrolidinyl)C₀-C₄alkyl, (morpholinyl)C₀-C₄alkyl, (thiomorpholinyl)C₀-C₄alkyl, (piperidinyl)C₀-C₄alkyl, (piperazinyl)C₀-C₄alkyl, (tetrahydrofuranyl)C₀-C₄alkyl, (pyrazolyl)C₀-C₄alkyl, (thiazolyl)C₀-C₄alkyl, (triazolyl)C₀-C₄alkyl, (tetrazolyl)C₀-C₄alkyl, (imidazolyl)C₀-C₄alkyl, (oxazolyl)C₀-C₄alkyl, (furanyl)C₀-C₄alkyl, (pyridinyl)C₀-C₄alkyl, (pyrimidinyl)C₀-C₄alkyl, (pyrazinyl)C₀-C₄alkyl, (pyridizinyl)C₀-C₄alkyl, and (tetrahydropyridinyl)C₀-C₄alkyl; which may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
This disclosure includes compounds and salts in which one of R¹²and R¹³is hydrogen, hydroxyl, halogen, methyl, or methoxy; the other of R¹²and R¹³is chosen from (s) where (s) is chosen from -JNR⁹C(O)OR¹⁰, -JNR⁹C(O)OR²³, -JOC(O)NR²¹R²², JOC(O)NR²⁴R²⁵, JNR⁹C(O)NR¹⁰R²³, and -JNR⁹C(O)NR²⁴R²⁵;
R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl, (pyrrolidinyl)C₀-C₄alkyl, ((morpholinyl)C₀-C₄alkyl, (thiomorpholinyl)C₀-C₄alkyl, (piperidinyl)C₀-C₄alkyl, (piperazinyl)C₀-C₄alkyl, (tetrahydrofuranyl)C₀-C₄alkyl, pyrazolyl)C₀-C₄alkyl, (thiazolyl)C₀-C₄alkyl, (triazolyl)C₀-C₄alkyl, (tetrazolyl)C₀-C₄alkyl, (imidazolyl)C₀-C₄alkyl, (oxazolyl)C₀-C₄alkyl, (furanyl)C₀-C₄alkyl, (pyridinyl)C₀-C₄alkyl, (pyrimidinyl)C₀-C₄alkyl, (pyrazinyl)C₀-C₄alkyl, (pyridizinyl)C₀-C₄alkyl, and (tetrahydropyridinyl)C₀-C₄alkyl;
R²³is independently chosen at each occurrence from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (pyrrolidinyl)C₀-C₄alkyl, (morpholinyl)C₀-C₄alkyl, (thiomorpholinyl)C₀-C₄alkyl, (piperidinyl)C₀-C₄alkyl, (piperazinyl)C₀-C₄alkyl, (tetrahydrofuranyl)C₀-C₄alkyl, (pyrazolyl)C₀-C₄alkyl, (thiazolyl)C₀-C₄alkyl, (triazolyl)C₀-C₄alkyl, (tetrazolyl)C₀-C₄alkyl, (imidazolyl)C₀-C₄alkyl, (oxazolyl)C₀-C₄alkyl, (furanyl)C₀-C₄alkyl, (pyridinyl)C₀-C₄alkyl, (pyrimidinyl)C₀-C₄alkyl, (pyrazinyl)C₀-C₄alkyl, (pyridizinyl)C₀-C₄alkyl, and (tetrahydropyridinyl)C₀-C₄alkyl;
R²⁴and R²⁵are taken together with the nitrogen to which they are attached to form a pyrrolidinyl, piperazinyl, piperidinyl, or morpholinyl group, each of which is optionally bridged with a methylene or ethylene group or spiro to a C₃-C₅cycloalkyl group; each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH, —CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy.
This disclosure includes compounds and salts of Formula IA:
where
B may carry any of the definitions set forth herein for this variable. In certain embodiments B is a 2-fluoro-3-chlorophenyl or a 2-fluoro-3-trifluoromethoxy-phenyl. Examples of such compounds include the compounds shown in Table 1. In any of the compounds shown in Table 1 the 2-fluoro-3-chloro-phenyl group may be replaced by a 2-fluoro-3-trifluoromethoxy-phenyl.
This disclosure includes compounds and salts of Formula IB, IC, and ID.
In Formula IB, IC, and ID the variables may include any of the definitions set forth herein that results in a stable compound. In certain embodiments the following conditions apply for Formula IB, IC, and ID.
R¹is hydrogen and R²is fluoro.
R¹and R²are joined to form a 3 membered ring.
m is 0.
B is pyridyl, optionally substituted with halogen, C₁-C₂alkoxy, and trifluoromethyl.
B is phenyl, substituted with 1, 2, or 3 substituents independently selected from halogen, C₁-C₂alkyl, C₁-C₂alkoxy, trifluoromethyl, and optionally substituted phenyl.
R¹³is hydrogen and R¹²is —NHC(O)NR²⁴R²⁵.
R¹³is hydrogen and R¹²is —CCR²³.
R¹³is hydrogen and R¹²is —NHC(O)NHR²³.
R¹³is hydrogen and R¹²is —C(O)R²³.

TABLE 1

The disclosure also includes compounds and salts of Formula IA in which R¹³is hydrogen, halogen, hydroxyl, nitro, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (C₃-C₇cycloalkyl)C₀-C₄alkoxy, C₁-C₂haloalkyl, or C₁-C₂haloalkoxy and B is an aryl or heteroaryl group such as a phenyl or pyridyl substituted with at least one phosphate, thiophosphate, phosphoamide, or phosphothioamide group and optionally substituted with additional substituents independently chosen from halogen, hydroxyl, nitro, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (C₃-C₇cycloalkyl)C₀-C₄alkoxy, C₁-C₂haloalkyl, or C₁-C₂haloalkoxy.
Examples of such compounds include:
The disclosure also includes boron containing compounds. These compounds may be compounds of Formula IA in which the R¹³substituent is a boron containing substituent such as —B(OH)₂and also compounds of Formula I in which A is a benzoazoborale group. For example
The disclosure also includes compounds which contain an azulene ring. These compounds are compounds of Formula IE. In certain embodiments B is a 2-fluoro-3-chlorophenyl or a 2-fluoro-3-trifluoromethoxy-phenyl. In any of the azulene containing compounds shown below the 2-fluoro-3-chloro-phenyl group may be replaced by a 2-fluoro-3-trifluoromethoxy-phenyl.
The disclosure includes compounds of Formula I in which R¹and R²are joined to a 3- to 6-membered heterocycloalkyl group and R^1′, R^2′, R³, and R^3′are all hydrogen. The disclosure also includes compounds in which R²and R³are joined to a 3- to 6-membered heterocycloalkyl group and R¹, R^1′, R^2′, and R^3′are all hydrogen.
Examples of such compounds include:
The disclosure further includes compounds and salts of Formula I in which the group A is 2-fluoro-3-chloro-phenyl. The 2-fluoro-3-chloro-phenyl group may be replaced by another carbocyclic or heterocyclic group such as 2-bromo-pyridin-6-yl, 1-(2,2,2-trifluroehtyl)-1H-pyrazol-3-yl, 2,2-dichlorocyclopropylmethyl, 2-fluoro-3-trimethylsilylphenyl.
The disclosure further includes compounds and salts of Formula I, in which A is a heterocyclic group chosen from:
Examples of such compounds include:
Additional compounds of the disclosure include:
in which R is methyl, or amino and X is N, CH or CF.
The disclosure includes compounds of Formula I in which the central pyrrolidine is vinyl substituted.
The disclosure includes compounds of Formula I, in which the central pyrrolidine is modified. A modification to the central pyrrolidine that is within the scope of the disclosure is the addition of a second heteroatom to the pyrrolidine ring, such as N, O, S, or Si. Another modification within the scope of the disclosure is joining a substituent on the central pyrrolidine ring to R⁷or R⁸to form a 5- to 6-membered heterocyclic ring. Examples of these modifications include:
Prodrugs of Formula I are also within the scope of the disclosure. Prodrugs of Formula I include compounds in which the R₁₃substituent is a long-chain phosphoether group. Examples of such compounds include:

Pharmaceutical Preparations

Compounds disclosed herein can be administered as the neat chemical, but are preferably administered as a pharmaceutical composition. Accordingly, the disclosure provides pharmaceutical compositions comprising a compound or pharmaceutically acceptable salt of Formula I, together with at least one pharmaceutically acceptable carrier. The pharmaceutical composition/combination may contain a compound or salt of Formula I as the only active agent, but is preferably contains at least one additional active agent. In certain embodiments the pharmaceutical composition is in a dosage form that contains from about 0.1 mg to about 2000 mg, from about 10 mg to about 1000 mg, from about 100 mg to about 800 mg, or from about 200 mg to about 600 mg of a compound of Formula I and optionally from about 0.1 mg to about 2000 mg, from about 10 mg to about 1000 mg, from about 100 mg to about 800 mg, or from about 200 mg to about 600 mg of an additional active agent in a unit dosage form. The pharmaceutical composition may also include a molar ratio of a compound of Formula I and an additional active agent. For example the pharmaceutical composition may contain a molar ratio of about 0.5:1, about 1:1, about 2:1, about 3:1 or from about 1.5:1 to about 4:1 of an another anti-inflammatory agent.
Compounds disclosed herein may be administered orally, topically, parenterally, by inhalation or spray, sublingually, transdermally, via buccal administration, rectally, as an ophthalmic solution, or by other means, in dosage unit formulations containing conventional pharmaceutically acceptable carriers. The pharmaceutical composition may be formulated as any pharmaceutically useful form, e.g., as an aerosol, a cream, a gel, a pill, a capsule, a tablet, a syrup, a transdermal patch, or an ophthalmic solution. Some dosage forms, such as tablets and capsules, are subdivided into suitably sized unit doses containing appropriate quantities of the active components, e.g., an effective amount to achieve the desired purpose.
Carriers include excipients and diluents and must be of sufficiently high purity and sufficiently low toxicity to render them suitable for administration to the patient being treated. The carrier can be inert or it can possess pharmaceutical benefits of its own. The amount of carrier employed in conjunction with the compound is sufficient to provide a practical quantity of material for administration per unit dose of the compound.
Classes of carriers include, but are not limited to binders, buffering agents, coloring agents, diluents, disintegrants, emulsifiers, flavorants, glidents, lubricants, preservatives, stabilizers, surfactants, tableting agents, and wetting agents. Some carriers may be listed in more than one class, for example vegetable oil may be used as a lubricant in some formulations and a diluent in others. Exemplary pharmaceutically acceptable carriers include sugars, starches, celluloses, powdered tragacanth, malt, gelatin; talc, and vegetable oils. Optional active agents may be included in a pharmaceutical composition, which do not substantially interfere with the activity of the compound of the present invention.
The pharmaceutical compositions/combinations can be formulated for oral administration. These compositions contain between 0.1 and 99 weight % (wt. %) of a compound of Formula I and usually at least about 5 wt. % of a compound of Formula. Some embodiments contain from about 25 wt. % to about 50 wt. % or from about 5 wt. % to about 75 wt. % of the compound of Formula I.

Methods of Treatment

The compounds and pharmaceutical compositions disclosed herein are useful for treating and preventing inflammatory disorders and disorders of the Complement cascade in patients. Inflammatory disorders that may be treated or prevented by the compounds and compositions of this disclosure include inflammatory effects of sepsis, systemic inflammatory response syndrome (SIRS), ischemia/reperfusion injury (I/R injury), psoriasis, myasthenia gravis, system lupus erythematosus (SLE), paroxysmal nocturnal hemoglobinuria (PNH), hereditary angioedema, multiple sclerosis, trauma, burn injury, capillary leak syndrome, obesity, diabetes, Alzheimer's dementia, stroke, schizophrenia, epilepsy, age-related macular degeneration, glaucoma, diabetic retinopathy, asthma, allergy, acute respiratory distress syndrome (ARDS), atypical hemolytic uremic syndrome (aHUS), hemolytic uremic syndrome (HUS), cystic fibrosis, myocardial infarction, lupus nephritides, Crohn's disease, rheumatoid arthritis, atherosclerosis, transplant rejection, prevention of fetal loss, biomaterial reactions (e.g. in hemodialysis, inplants), C3 glomerulonephritis, abdominal aortic aneurysm, and vasculitis. “Prevention” as used in this disclosure means decreasing the likelihood of the appearance of symptoms in a patient administered the compound prophylactically as compared to the likelihood of the appearance of symptoms in patients not administered the compound or decreasing the severity of symptoms in a patient administered the compound prophylactically as compared to the severity of symptoms experienced by patients with the disorder or condition who were not administered the compound.
This disclosure provides methods of treating or preventing an inflammatory disorder, by providing an effective amount of a compound or pharmaceutically acceptable salt of Formula I to patient infected with a Factor D mediated inflammatory disorder. A compound or salt of Formula I may be provided as the only active agent or may be provided together with one or more additional active agents.
An effective amount of a pharmaceutical composition/combination of the invention may be an amount sufficient to (a) inhibit the progression of an inflammatory disorder; (b) cause a regression of the inflammatory disorder; or (c) cause a cure of inflammatory disorder.
An effective amount of a compound or pharmaceutical composition described herein will also provide a sufficient concentration of the active agent when administered to a patient to provide a clinical benefit. Such an amount may be ascertained experimentally, for example by assaying blood concentration of the agent, or theoretically, by calculating bioavailability. The amount of an active agent sufficient to inhibit an inflammatory disorder may be determined with a conventional assay for Complement Factor D inhibition.

EXAMPLES

Abbreviations

AcOEt, EtOAc ethyl acetate
ACN acetonitrile
(Boc)₂O di-tert-butyl dicarbonate
DCM dichloromethane

DIEA, DIPEA N,N-diisopropylethylamine

DMA N,N-dimethylacetamide

DMF N,N-dimethylformamide

DPPA diphenyl phosphoryl azide
HATU O-(7-Azabenzotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate
NEt₃triethylamine
ⁱPr₂NEt N,N-diisopropylethylamine
TEA triethylamine
TFA trifluoroacetic acid
Tf₂O trifluoromethanesulfonic anhydride
THE tetrahydrofuran
tBuOK potassium tert-butoxide
TMSBr bromotrimethylsilane

General Methods

All nonaqueous reactions were performed under an atmosphere of dry argon gas using oven-dried glassware and anhydrous solvents. The progress of reactions and the purity of target compounds were determined using one of the following two UPLC methods: (1) Waters ACQUITY UPLC BEH C18 1.7 μm 2.1×50 mm column with an isocratic elution of 0.24 min at 85:15 water:acetonitrile containing 0.05% formic acid followed by a 3.26-min linear gradient elution from 85:15 to 15:85 at a flow rate of 0.8 mL/min with UV (PDA), ELS, and MS (SQ in EI mode) detection (method 1); and (2) Waters ACQUITY UPLC BEH C18 1.7 μm 2.1×150 mm column with an isocratic elution of 0.31 min at 95:5 water:acetonitrile containing 0.05% formic acid followed by a 16.40-min linear gradient elution from 95:5 to 10:90 at a flow rate of 0.43 mL/min with UV (PDA), ELS, and MS (SQ in EI mode) detection (method 2).

Example 1. Synthesis of Intermediates

1A. (2S,4R)-tert-butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidine-1-carboxylate

(2S,4R)-1-(tert-butoxycarbonyl)-4-fluoropyrrolidine-2-carboxylic acid (2.33 gm, 10 mmol) was dissolved in DMF (50 ml) and ⁱPr₂NEt (8.6 ml, 5 eq.) was added, followed by the addition of (3-chloro-2-fluorophenyl) methanamine (3.18 gm 20 mmol) at 5° C. Then HATU (8 gm, 2.1 eq) was added slowly at same temperature. The reaction mixture was then stirred for 18 h at RT. After completion of the reaction monitored by HPLC, The reaction mixture was diluted with 1M citric acid solution (200 ml+NaCl solid 20 gm) and extracted with DCM (150 mL×2), the organic layer was then washed with an aqueous solution of NaHCO₃(100 ml) and washed with water (100 ml), brine (100 ml) and dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by column chromatography (eluted with DCM/EtOAc) to give (2S,4R)-tert-butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidine-1-carboxylate.

1B. (2S,4R)—N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide hydrochloride (A)

(2S,4R)-tert-butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidine-1-carboxylate (500 mg,) was taken in 4N HCl dioxane (30 ml) and resulting reaction mixture was stirred at rt for 3 h. After completion of the reaction monitored by HPLC solvent was removed under reduced pressure. The residue, A, was used for next reaction.

1C. 2-(3-acetyl-1H-indol-1-yl)acetic acid (B)

A mixture of 3-Acetylindole (10.09 g) and t-butylbromoacetate (13.71 g) were reflux in acetonitrile in presence of potassium carbonate (9.7 g) for 24 h. The reaction mixture was cooled to room temperature and filtered and evaporated to dryness. The residue was purified by chromatography over silica gel and eluted with a mixture of ethylacetate in methylene chloride to give tert-butyl 2-(3-acetyl-1H-indol-1-yl)acetate.
Tert-butyl 2-(3-acetyl-1H-indol-1-yl)acetate was stirred overnight in a mixture of trifluoroacetic acid in methylene chloride and diluted with methanol and evaporated to dryness. The residue was treated with 1M sodium hydroxide and extracted with methylene chloride. The aqueous layer was acidified with 6M HCl and the residue filtered, washed with water and dried to give 2-(3-acetyl-1H-indol-1-yl)acetic acid (B).

1D. 1-(3-amino-1H-indol-1-yl)ethanone hydrochloride (C)

To a stirred solution of AgNO₃(9.3 g, 1.07 equiv) in acetonitrile was added benzoyl chloride (7.47 g, 1.04 equiv) dropwise at 0° C. The mixture was stirred for 10 min, and then solution of 1H-indole (6 g, 1 equiv) in acetonitrile was added at 0° C. and stirred for 1 h at RT. The reaction mixture was poured into ice to get a dark brown precipitate. The precipitate was filtered washed with water dried. The crude residue was purified by flash column chromatography (ISCO with hexanes/EtOAc) to give 3-nitro-1H-indole.
A stirred solution of 3-nitro-1H-indole (1 g, 1 equiv) in dry THF was cooled to 5° C. Then tBuOK (830 mg, 1.2 equiv) was added slowly, and the resulting mixture was stirred 10 min. Acetyl chloride (525 mg, 1.2 equiv) was added and the reaction mixture was stirred for 30 min. After completion of the reaction as monitored by HPLC, solvent was removed under reduced pressure, diluted with DCM washed with an aqueous solution of NaHCO₃. The organic layer was separated washed with brine and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/EtOAc) to give 1-(3-nitro-1H-indol-1-yl)ethanone.
To a mixture of 1-(3-nitro-1H-indol-1-yl)ethanone (600 mg, 2.942 mmol) and di-tert-butyl dicarbonate (1.925 g, 8.826 mmol) in methanol (50 mL) was added 5% Pd/C (20 mg). The resulting reaction mixture was stirred under hydrogen 24 h, after completion of the reaction Pd/C was filtered using celite, and the filtrate was concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/EtOAc) to give tert-butyl (1-acetyl-1H-indol-3-yl)carbamate.
Tert-butyl (1-acetyl-1H-indol-3-yl) carbamate (300 mg, 1.1 mmol) was taken in 4N HCl dioxane (10 ml) and resulting reaction mixture was stirred at rt for 3 h. After completion of the reaction solvent was removed under reduced pressure. This material, 1-(3-amino-1H-indol-1-yl)ethanone hydrochloride (C), was used directly in the next synthetic step.

1E. 2-(3-acetyl-6-hydroxy-1H-indol-1-yl)acetic acid (D)

6-Benzyloxyindole was acetylated using reported procedure (Eur. J. Med Chem., (2011), 46, 756) and alkylated following the procedure describe for compound B. The benzyl group was removed by hydrogenation over palladium on charcoal and the t-butyl group removed again as described for compound B.

1F. 2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetic acid (E)

To a stirred solution of 5-(benzyloxy)-1H-indole (11.08 g, 1 equiv) in 200 mL DCM was added diethylaluminium chloride (1 M solution in Hexane; 74.6 mL, 1.5 equiv) drop wise at 0° C. The mixture was stirred for 30 min, and then a solution of acetyl chloride (5.3 mL, 1.5 equiv) in 150 mL DCM was added at 0° C. and the reaction was stirred for 1 h at 0° C. A 5% aqueous citric acid solution was added at 0° C. and the reaction was stirred for 15 min at RT. The precipitate was filtered and washed with water, and the organic filtrate was dried and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give 1-(5-(Benzyloxy)-1H-indol-3-yl)ethanone.
To a suspension of 1-(5-(benzyloxy)-1H-indol-3-yl)ethanone (6.5 gm, 1 equiv) and K₂CO₃(3.72 gm, 1.1 equiv) in 50 mL acetonitrile was added tert-butyl 2-bromoacetate (3.92 mL, 1.1 equiv) dropwise at RT. The resulting mixture was then heated to reflux for 18 h. After cooling to RT, the mixture was diluted with DCM (100 mL), and then filtered through the celite pad; filtrate was concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (silica gel eluted with DCM/EtOAc) to give tert-Butyl 2-(3-acetyl-5-(benzyloxy)-1H-indol-1-yl)acetate.
To tert-Butyl 2-(3-acetyl-5-(benzyloxy)-1H-indol-1-yl)acetate (6 g) in THF (80 mL) was added Pd/C (0.05 equiv). The reaction mixture was stirred at RT for 5 h under H₂(1 atm). The reaction mixture was then filtered through a pad of Celite and washed with CH₂Cl₂and MeOH. The filtrate was concentrated under reduced pressure and the remaining residue was purified by flash column chromatography (silica gel eluted with DCM/EtOAc) to give tert-Butyl 2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetate.
Tert-Butyl 2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetate (814 mg, 2.8 mmol) was taken up in 4 N HCl dioxane (10 mL) and the resulting reaction mixture was stirred at RT for 48 h. The solvent was then removed under reduced pressure to give 2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetic acid (E) which could be used directly in the next synthetic step.

Example 2. Synthesis of (2S,3aS,7aS)-1-(2-(3-acetyl-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)octahydro-1H-indole-2-carboxamide. (Compound 1)

1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (296 mg) was added in portions to a cooled (0° C.) mixture of (2S,3aS,7aS)-1-(tert-butoxycarbonyl)octahydro-1H-indole-2-carboxylic acid (200 mg), 3-chloro-2-fluorobenzylamine (124 mg), and N,N-diisopropylethylamine (520 μL) in N,N-dimethylformamide (5 mL). The resulting mixture was stirred for 45 min at 0° C., warmed to rt, and stirred at rt for 1.5 h. The reaction mixture was diluted with a 1 M aq. solution of citric acid (50 mL) and extracted with dichloromethane (3×100 mL). The combined organic extracts were concentrated to approximately 50 mL under reduced pressure, washed with a saturated aq solution of sodium bicarbonate (2×25 mL), washed with brine (25 mL), dried over sodium sulfate, and evaporated under reduced pressure. The remaining residue was purified by flash column chromatography on silica (ethyl acetate/hexanes gradient, 0 to 60% v/v) to give (2S,3aS,7aS)-tert-Butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)octahydro-1H-indole-1-carboxylate as a solid. LC-MS (method 1): t_R2.63 min, m/z found 411 ([M+H]⁺).
(2S,3aS,7aS)-tert-Butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)octahydro-1H-indole-1-carboxylate (105 mg) was dissolved in a hydrogen chloride solution (4.0 M in dioxane, 5 mL). After stirring at rt for 1 h, the reaction mixture was evaporated under reduced pressure to give a solid, which was then treated with methanol (10 mL) and evaporated under reduced pressure. Treatment with methanol and subsequent evaporation was repeated twice. The resulting solid, (2S,3aS,7aS)—N-(3-Chloro-2-fluorobenzyl)octahydro-1H-indole-2-carboxamide hydrochloride, was dried in vacuo overnight and used directly in the next synthetic step. LC-MS (method 1): t_R1.28 min, m/z found 311 ([M+H]⁺).
1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (98 mg) was added in one portion to a mixture of (2S,3aS,7aS)—N-(3-chloro-2-fluorobenzyl)octahydro-1H-indole-2-carboxamide hydrochloride (from above), compound B (56 mg), and N,N-diisopropylethylamine (180 μL) in N,N-dimethylformamide (2 mL) at rt. The resulting mixture was stirred for 15 min and concentrated under reduced pressure to approximately half the original volume. The resulting mixture was diluted with ethyl acetate (40 mL), washed with a 1 M aq solution of citric acid (10 mL), washed with a saturated aq solution of sodium bicarbonate (10 mL), washed with brine (10 mL), dried over sodium sulfate, and evaporated under reduced pressure. The remaining residue was purified by flash column chromatography on silica (methanol/dichloromethane gradient, 0 to 10% v/v) to give, (2S,3aS,7aS)-1-(2-(3-Acetyl-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)octahydro-1H-indole-2-carboxamide, as a solid foam. LC-MS (method 1): t_R2.33 min, m/z found 510 ([M+H]⁺).

Example 3. Synthesis of (2S,4R)—N2-(1-acetyl-1H-indol-3-yl)-N1-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-1,2-dicarboxamide (Compound 3)

1-(3-amino-1H-indol-1-yl)ethanone hydrochloride (C). (411 mg 1.96 mmol) was dissolved in DMF (25 ml) and ⁱPr₂NEt (900 μl, 5 eq.) was added, which was followed by the addition of (2S,4R)-1-(tert-butoxycarbonyl)-4-fluoropyrrolidine-2-carboxylic acid (456 mg, 1.96 mmol) at 5° C. Then HATU (878 mg, 2.1 eq) was added slowly at same temperature. The reaction mixture was then stirred for 18 h at RT. After completion of the reaction monitored by HPLC, the reaction mixture was diluted with 1M citric acid solution (100 ml+NaCl solid 10 gm) and extracted with DCM (50 mL×2). The organic layer was then washed with an aqueous solution of NaHCO₃(40 ml), washed with water (40 ml), and then brine (40 ml), and dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/EtOAc) to give (2S,4R)-tert-butyl 2-((1-acetyl-1H-indol-3-yl)carbamoyl)-4-fluoropyrrolidine-1-carboxylate.
(2S,4R)-tert-butyl 2-((1-acetyl-1H-indol-3-yl)carbamoyl)-4-fluoropyrrolidine-1-carboxylate (78 mg,) was taken in 4N HCl dioxane (5 ml) and resulting reaction mixture was stirred at rt for 3 h. after completion of the reaction monitored by HPLC solvent was removed under reduced pressure. The residue, (2S,4R)—N-(1-acetyl-1H-indol-3-yl)-4-fluoropyrrolidine-2-carboxamide hydrochloride, was used as is for the next reaction.
(2S,4R)—N-(1-acetyl-1H-indol-3-yl)-4-fluoropyrrolidine-2-carboxamide hydrochloride was dissolved in THF (5 ml) and NEt₃(140 μl, 5 eq.) was added, which was followed by the addition of at 5° C. Then 1-chloro-2-fluoro-3-(isocyanatomethyl) benzene (100 mg, 2.1 eq, freshly prepared from 2-(3-chloro-2-fluorophenyl) acetic acid) was added slowly at same temperature. The reaction mixture was then stirred for 30 min at RT. After completion of the reaction monitored by HPLC, The reaction mixture was diluted with 5 ml water and extracted with ethyl acetate (50 ml), the organic layer dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (eluted with DCM/EtOAc) to give (2S,4R)—N2-(1-acetyl-1H-indol-3-yl)-N1-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-1,2-dicarboxamide. HPLC: t_R2.07 min. MS m/z calcd for C₂₃H₂₁ClF₂N₄O₃([M]⁺), 474; found, 475 ([M+1]⁺). ¹H-NMR (DMSO-D6, 400 MHz): δ2.2-2.1 (m, 1H), 2.60 (s, 3H), 3.79-3.72 (m, 2H), 4.34-4.28 (m, 2H), 4.72 (t, J=9.2 Hz 1H), 4.52-5.38 (m, 1H), 7.18-7.09 (m, 2H), 7.46-7.30 (m, 4H), 7.92 (d, J=7.6 Hz, 1H), 8.14 (s, 1H), 8.36 (d, J=8.4 Hz, 1H);), 10.3 (s, 1H).

Example 4. Synthesis of (2S,4R)—N-(3-chloro-2-fluorobenzyl)-1-(2-(3-(cyclopropanecarbonyl)-1H-indol-1-yl)acetyl)-4-fluoropyrrolidine-2-carboxamide. (Compound 25)

To a suspension of cyclopropyl(1H-indol-3-yl)methanone (3 gm, 16.22 mmol) and potassium carbonate (2.463 gm, 1.1 equiv) in 50 ml acetonitrile was added tert-butyl 2-bromoacetate (2.6 ml, 1.1 equiv) dropwise at RT. The resulting mixture was then heated to reflux for 18 h. After cooling to RT, the mixture was diluted with DCM (100 ml), and then filtered through the celite pad; filtrate was concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (eluted with DCM/EtOAc) to give tert-butyl 2-(3-(cyclopropanecarbonyl)-1H-indol-1-yl)acetate.
Tert-butyl 2-(3-(cyclopropanecarbonyl)-1H-indol-1-yl)acetate (198 mg, 0.66 mmol) was dissolved in CH₂Cl₂(5 mL) and TFA (5 mL) was added slowly at 5° C. The reaction mixture was stirred 3 h at RT. After completion of the reaction monitored by HPLC solvent was removed under reduced pressure. The residue, 2-(3-(cyclopropanecarbonyl)-1H-indol-1-yl)acetic acid was used for next reaction.
2-(3-(cyclopropanecarbonyl)-1H-indol-1-yl)acetic acid was dissolved in DMF (10 ml) and ⁱPr₂NEt (490 μl, 5 eq.) was added, which was followed by the addition of A (187 mg, 0.6 mmol) at 5° C. Then HATU (478 mg, 2.1 eq) was added slowly at same temperature. The reaction mixture was then stirred for 18 h at RT. After completion of the reaction monitored by HPLC, the reaction mixture was diluted with water and extracted with DCM (50 mL). The organic layer was then washed with an aqueous solution of NaHCO₃(20 ml) and washed with water (20 ml), brine (20 ml) and dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (eluted with DCM/EtOAc) to give (200 mg). HPLC: t_R2.02 min. MS m/z calcd for C₂₆H₂₄ClF₂N₃O₃([M]⁺), 499; found, 500 ([M+1]⁺). ¹H-NMR (DMSO-D6, 400 MHz): δ, 0.91-0.87 (m, 2H), 1.00-0.98 (m, 2H), 2.2-2.0 (m, 1H), 2.59-2.57 (m, 2H), 3.9-4.05 (m, 1H), 4.47-4.28 (m, 4H), 5.22-5.17 (m, 1H), 5.42-5.38 (m, 2H), 6.99-6.95 (m, 1H), 7.23-7.19 (m, 3H), 7.48-7.40 (m, 1H), 8.22-8.20 (m, 1H), 8.42 (s, 1H), 8.6-8.5-(m, J=8. 1H).

Example 5. Synthesis of (S)—N1-(1-carbamoyl-1H-indol-3-yl)-N2-(3-chloro-2-fluorobenzyl)indoline-1,2-dicarboxamide. (Compound 2)

(S)-1-(tert-butoxycarbonyl)indoline-2-carboxylic acid (2.63 gm, 10 mmol) was dissolved in DMF (50 ml) and ⁱPr₂NEt (8.6 ml, 5 eq.) was added, which was followed by the addition of (3-chloro-2-fluorophenyl) methanamine (3.18 gm 20 mmol) at 5° C. Then HATU (8 gm, 2.1 eq) was added slowly at same temperature. The reaction mixture was then stirred for 18 h at RT. After completion of the reaction monitored by HPLC, The reaction mixture was diluted with 1M citric acid solution (200 ml+NaCl solid 20 gm) and extracted with DCM (150 mL×2), the organic layer was then washed with an aqueous solution of NaHCO₃(100 ml) and washed with water (100 ml), brine (100 ml) and dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/EtOAc) to give (S)-tert-butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)indoline-1-carboxylate.
(S)-tert-butyl 2-((3-chloro-2-fluorobenzyl)carbamoyl)indoline-1-carboxylate (300 mg,) was taken in 4N HCl dioxane (30 ml) and resulting reaction mixture was stirred at rt for 3 h. After completion of the reaction monitored by HPLC solvent was removed under reduced pressure. The residue, (S)—N-(3-chloro-2-fluorobenzyl)indoline-2-carboxamide hydrochloride, was used for next reaction.
(S)—N-(3-chloro-2-fluorobenzyl)indoline-2-carboxamide hydrochloride (50 mg, 0.147 mmol) was dissolved in THF (5 ml) and NEt₃(100 μl, 5 eq.) was added, which was followed by the addition of 3-isocyanato-1H-indole-1-carboxamide (33 mg, 1.1 eq, freshly prepared) added slowly at 5° C. The reaction mixture was then stirred for 30 min at RT. After completion of the reaction monitored by HPLC, the reaction mixture was diluted with 5 ml water and extracted with ethyl acetate (50 ml), the organic layer dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/CH₃OH) to give (S)—N1-(1-carbamoyl-1H-indol-3-yl)-N2-(3-chloro-2-fluorobenzyl)indoline-1,2-dicarboxamide. HPLC: t_R2.04 min. MS m/z calcd for C₂₆H₂₁ClFN₅O₃([M]⁺), 505; found, 506 ([M+1]⁺). ¹H-NMR (DMSO-D6, 400 MHz): δ, 3.19-3.15 (m, 1H), 3.36 (s, 3H), 3.6-3.7 (m, 1H), 4.43 (d, J=5.6 Hz, 2H), 5.45-5.41 (m, 1H),), 6.88-6.86 (m, 1H), 6.90-6.88 (m, 1H), 7.34-6.97 (m, 5H), 7.45-7.43 (m, 1H), 7.50 (br, 2H), 7.68 (d, J=7.6 Hz, 1H), 7.91 (d, J=8 Hz, 1H), 8.11 (s, 1H), 8.35 (d, J=8.4 Hz 1H); 8.65 (s, 1H). 8.81-8.75 (m, 1H).

Example 6. Synthesis of (2S,4R)-1-(2-(3-acetyl-6-hydroxy-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide

Compounds A (3.6 g) and D (2.5 g) were coupled following procedure described in Example 2 to give (2S,4R)-1-(2-(3-acetyl-6-hydroxy-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide.

Example 7. Synthesis of diethyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate (Compound 48)

Under an atmosphere of argon gas, trifluoromethanesulfonic anhydride (250 μL) was added dropwise to a cooled (0° C.) solution of (2S,4R)-1-(2-(3-acetyl-6-hydroxy-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (551 mg) in pyridine (10 mL). The resulting solution was stirred at 0° C. for 2.5 h, allowed to warm to rt, and concentrated under reduced pressure to give an oil. This material was dissolved in ethyl acetate (75 mL), and the resulting solution was washed with a 1 M aq. solution of citric acid (2×25 mL), washed with brine (25 mL), dried over sodium sulfate, and evaporated under reduce pressure to give the crude product. This material was purified by flash column chromatography on silica (methanol/dichloromethane gradient, 0 to 5% v/v) to give, 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl trifluoromethanesulfonate, as a solid. LC-MS (method 1): t_R2.39 min, m/z found 622 ([M+H]⁺).
Under an atmosphere of argon gas, a mixture of 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl trifluoromethanesulfonate (526 mg), diethyl phosphite (1.2 mL), triethylamine (217 μL), and tetrakis(triphenylphosphine)palladium(0) (100 mg) in tetrahydrofuran (30 mL) was stirred at 100° C. in a sealed tube for 18 h. The reaction mixture was allowed to cool to room temperature and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography on silica (methanol/dichloromethane gradient, 0 to 5% v/v) to give, diethyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate, (as a solid. LC-MS (method 1): t_R1.84 min, m/z found 610 ([M+H]⁺). LC-MS (method 2): t_R7.15 min, m/z found 610 ([M+H]⁺).

Example 8. Synthesis of ethyl hydrogen (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate. (Compound 52)

Under an atmosphere of argon gas at rt, bromotrimethylsilane (132 mg) was added to a solution of diethyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate (200 mg) in dichloromethane (5 mL) at rt. The resulting solution was stirred for 3 h and evaporated to dryness under reduced pressure. The reaction was stopped at 3 h when the majority of the product was the monoethyl phosphonate. The residue was treated with a mixture of dichloromethane and methanol (3:1 v/v, 15 mL) and evaporated under reduced pressure. This treatment was repeated once, and the remaining solid was washed with ethyl acetate (15 mL), and dried in vacuo overnight. Ethyl hydrogen (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate was purified by HPLC to give 25 mg of solid. LC-MS: t_R1.20 min, m/z found 582 ([M+H]⁺).

Example 9. Synthesis of (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonic acid. (Compound 68)

Under an atmosphere of argon gas at rt, bromotrimethylsilane (233 mg) was added to a solution of diethyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonate (150 mg) in dichloromethane (5 mL) at rt. The resulting solution was stirred for 18 h and evaporated to dryness under reduced pressure. The remaining residue was treated with a mixture of dichloromethane and methanol (3:1 v/v, 15 mL) and evaporated under reduced pressure. This treatment was repeated once, and the remaining solid was washed with ethyl acetate (15 mL), and dried in vacuo overnight to give 132 mg of (3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)phosphonic acid. LC-MS (method 1): t_R1.06 min, m/z found 554 ([M+H]⁺). LC-MS (method 2): t_R5.40 min, m/z found 554 ([M+H]⁺).

Example 10. Synthesis of diethyl(((3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)oxy)methyl)phosphonate (Compound 66)

(2S,4R)-1-(2-(3-acetyl-6-hydroxy-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (100 mg, 0.204 mmol), Tosylate (98.8 mg, 0.307 mmol), Cs₂CO₃(200 mg, 0.614 mmol) in dimethylformamide (2 mL) was stirred for 15 hr at 50° C. Solvent was removed in vacuo and the residue was purified by HPLC to afford diethyl(((3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-6-yl)oxy)methyl)phosphonate. LC-MS (method 1): t_R1.86 min, m/z found 640 ([M+H]⁺).

Example 11. Synthesis of 3-((2S,4R)-2-(5-(3-chlorophenyl)-1H-imidazol-2-yl)-4-fluoropyrrolidine-1-carboxamido)-1H-indole-1-carboxamide (Compound 11)

(2S,4R)-1-(tert-butoxycarbonyl)-4-fluoropyrrolidine-2-carboxylic acid (1.103 g, 4.73 mmol) and 2-bromo-1-(3-chlorophenyl)ethanone (1.007 g, 4.30 mmol) were dissolved in acetonitrile (20 ml), into which diisopropylethylamine (0.822 ml, 4.73 mmol) was added at rt and the mixture was stirred overnight. After solvent was removed on a rotavapor, the residue was dissolved in AcOEt and washed with water and brine. After removing AcOEt, (2S,4R)-1-tert-butyl 2-(2-(3-chlorophenyl)-2-oxoethyl) 4-fluoropyrrolidine-1,2-dicarboxylate was obtained as colorless syrups.
(2S,4R)-1-tert-butyl 2-(2-(3-chlorophenyl)-2-oxoethyl) 4-fluoropyrrolidine-1,2-dicarboxylate was mixed with ammoniumacetate (3.53 g, 45.8 mmol) and refluxed in toluene (25 ml) for 5 hr. The mixture was diluted with AcOEt and washed with water and brine. After removing solvent, the crude was purified by chromatography over silica gel. (2S,4R)-tert-butyl 2-(5-(3-chlorophenyl)-1H-imidazol-2-yl)-4-fluoropyrrolidine-1-carboxylate (1.58 g) was obtained as yellow foam.
(2S,4R)-tert-butyl 2-(5-(3-chlorophenyl)-1H-imidazol-2-yl)-4-fluoropyrrolidine-1-carboxylate (0.14 g, 0.38 mmol) was treated with TFA (1 ml) in DCM (4 ml) at rt for 2 hr. volatiles were removed by evaporation. The residue was coevaporated with tolene (10 ml×2) to get 5-(3-chlorophenyl)-2-((2S,4R)-4-fluoropyrrolidin-2-yl)-1H-imidazole.
5-(3-chlorophenyl)-2-((2S,4R)-4-fluoropyrrolidin-2-yl)-1H-imidazole is dissolved in THF (5 ml) and TEA (0.266 ml, 1.9 mmol). Into the mixture, 3-isocyanato-1H-indole-1-carboxamide (0.0844 g, 0.42 mmol) was added at rt. After 2 hr, the mixture was concentrated and the residue was purified by chromatography over silica gel to give 3-((2S,4R)-2-(5-(3-chlorophenyl)-1H-imidazol-2-yl)-4-fluoropyrrolidine-1-carboxamido)-1H-indole-1-carboxamide (0.110 g) as white foam. ¹H-NMR (DMSO-d₆, 400 MHz) 12.45 (s, 1H); 8.47 (s, 1H); 8.33 (d, J=8.4 Hz, 1H); 7.99 (s, 1H); 7.86 (s, 1H); 7.78-7.69 (m, 3H); 7.04-7.33 (m, 3H); 7.26-7.20 (m, 2H); 7.00 t, J=7.2 Hz); 5.512 (d, J=53 Hz, 1H); 5.25 (t, J=7.6 Hz, 1H); 4.40 (t, J=7.2 Hz, 1H); 4.13 (dd, J=22 Hz, 12 Hz, 1H); 3.84 (dd, J=37 Hz, 11 Hz, 1H); 2.74-2.66 (m, 2H).

Example 12. diethyl (1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-pyrazolo[3,4-c]pyridin-3-yl)phosphonate (Compound 61)

To a solution of 1H-pyrazolo [3,4-c]pyridine (4.94 g) in DMF (60 mL) were added iodine (15.8 gm) and potassium hydroxide (5.85 gm). The reaction mixture was stirred at RT for 16 h. The mixture was diluted with 10% sodium thiosulfate and water, then extracted (2×) with EtOAc. The combined organic extracts were washed with brine, then dried and concentrated under reduced pressure to give 3-lodo-1H-pyrazolo[3,4-c]pyridine.
To a suspension of 3-iodo-1H-pyrazolo[3,4-c]pyridine (6.89 g) and potassium carbonate (8.16 g) in acetonitrile (60 mL) was added tert-butyl bromoacetate (4.5 mL,) dropwise at RT and the resulting mixture was heated to reflux for 24 h. The mixture was cooled to RT and filtered, the solid was washed with CH₃CN and the filtrate was concentrated under vacuum. The remaining residue was purified by column chromatography (eluted with DCM/EtOAc) to give Tert-butyl 2-(3-iodo-1H-pyrazolo[3,4-c]pyridin-1-yl)acetate.
To a suspension of Tert-butyl 2-(3-iodo-1H-pyrazolo[3,4-c]pyridin-1-yl)acetate (100 mg, 0.278 mmol), Cesium carbonate (108 mg, 1.2 eq) and diethyl phosphonate (38 mg, 1.1 equiv) in 5 ml THF are added. After initial heating in microwave, the reaction temperature was maintained for 15 min at 115° C. After cooling to RT, reaction mixture was concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/CH₃OH) to give tert-butyl 2-(3-(diethoxyphosphoryl)-1H-pyrazolo[3,4-c]pyridin-1-yl)acetate (62 mg).
tert-butyl 2-(3-(diethoxyphosphoryl)-1H-pyrazolo[3,4-c]pyridin-1-yl)acetate (63 mg, 0.17 mmol) was taken in 4N HCl dioxane (5 ml) and resulting reaction mixture was stirred at rt for 24 h. After completion of the reaction monitored by HPLC, solvent was removed under reduced pressure. The residue, 2-(3-(diethoxyphosphoryl)-1H-pyrazolo[3,4-c]pyridin-1-yl)acetic acid, was used for next reaction.
2-(3-(diethoxyphosphoryl)-1H-pyrazolo[3,4-c]pyridin-1-yl)acetic acid (0.170 mmol) from previous reaction was dissolved in DMF (10 ml) and iPr₂NEt (140 μl, 5 equiv) was added This was followed by the addition of A (47.7 mg, 0.154 mmol) at 5° C. Then HATU (136 mg, 2.1 equiv) was added slowly at same temperature. The reaction mixture was then stirred for 18 h at RT. After completion of the reaction monitored by HPLC, The reaction mixture was diluted with water and extracted with DCM (50 mL), the organic layer was then washed with an aqueous solution of NaHCO₃(20 ml) and washed with water (20 ml), brine (20 ml) and dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (ISCO eluted with DCM/CH₃OH) to give diethyl (1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-pyrazolo[3,4-c]pyridin-3-yl)phosphonate (80 mg).). HPLC: tR 0.82 min. MS m/z calcd for C₂₄H₂₇ClF₂N₅O₅P ([M]+), 570; found, 572 ([M+2]+). 1H-NMR (DMSO-D6, 400 MHz): δ, 1.28-1.23 (m, 6H), 2.2-2.0 (m, 1H), 4.05-3.85 (m, 1H), 4.17-4.08 (m, 4H), 4.46-4.24 (m, 3H), 5.58-5.45 (m, 1H),), 5.71 (d, J=17.2 Hz, 1H), 5.94 (d, J=17.2 Hz, 1H), 7.00-6.96 (m, 1H), 7.21-7.19 (m, 1H), 7.44-7.40 (m, 1H), 7.85 (d, J=5.6 Hz, 1H), 8.40 (d, J=5.6 Hz, 1H), 8.62-8.59 (m, 1H), 9.16 (s, 1H).

Example 13. Synthesis of (1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-pyrazolo[3,4-c]pyridin-3-yl)phosphonic acid (Compound 65)

To a mixture of 27 (20 mg, 0.037 mmol) in 3 ml DCM under argon was added bromotrimethylsilane (54 μl, 10.5 equiv). The mixture was stirred at rt for 3 days and solvents were removed under reduced pressure. Then the product was dissolved in CH₃OH/DCM, then solvents removed under reduced pressure to yield 1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-pyrazolo[3,4-c]pyridin-3-yl)phosphonic acid quatintatively. HPLC: tR 0.72 min MS m/z calcd for C₂₀H₁₉ClF₂N₅O₅P ([M]+), 513; found, 514 ([M+1]+). ¹H-NMR (DMSO-d₆, 400 MHz): δ, 2.2-2.0 (m, 1H), 4.08-3.98 (m, 1H), 4.51-4.18 (m, 3H), 5.64-5.51 (m, 1H),), 5.35-5.79 (m, 3H), 6.03 (d, J=17.2 Hz, 1H), 7.07-7.03 (m, 1H), 7.28-7.24 (m, 1H), 7.48-7.45 (m, 1H), 8.30 (d, J=6 Hz, 1H), 8.57 (d, J=5.6 Hz, 1H), 8.69 (t, J=5.6 Hz 1H), 9.16 (s, 1H); 9.58 (s, 1H).

Example 14. Synthesis of 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl cyclopropylcarbamate (Compound 110)

2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetic acid (2.8 mmol) E was dissolved in DMF (20 mL) and ⁱPr₂NEt (2.076 mL, 5 equiv) was added, followed by the addition of (2S,4R)—N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide hydrochloride A (788 mg, 2.54 mmol) at 5° C. HATU (2.026 g, 2.1 equiv) was then added slowly at the same temperature and the reaction mixture was stirred for 18 h at RT. The reaction mixture was then diluted with 1 M citric acid solution (100 mL, containing 10 g NaCl) and extracted with DCM (2×50 mL). The organic layer was washed with an aqueous solution of NaHCO₃(40 mL), washed with water (40 mL), washed with brine (40 mL), dried over Na₂SO₄, and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (silica gel eluted with DCM/EtOAc) to give (2S,4R)—N-(3-chloro-2-fluorobenzyl)-1-(2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetyl)-4-fluoropyrrolidine-2-carboxamide.
(2S,4R)—N-(3-chloro-2-fluorobenzyl)-1-(2-(3-acetyl-5-hydroxy-1H-indol-1-yl)acetyl)-4-fluoropyrrolidine-2-carboxamide (220 mg, 1 equiv) was dissolved in THF (20 mL) and NEt₃(100 μl, 1.7 equiv) was added, followed by the addition of 4-nitrophenyl chloroformate (136 mg, 1.5 equiv) at 0° C. The reaction mixture was then stirred for 18 h at RT, diluted with 5 mL water, and extracted with ethyl acetate (50 mL). The organic layer was dried over Na₂SO₄and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (silica gel eluted with DCM/EtOAc) to give 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl (4-nitrophenyl) carbonate.
3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl (4-nitrophenyl) carbonate was dissolved in a mixture of THF (5 mL), ACN (5 mL), and cyclopropanamine (12.86 μl, 2 equiv), followed by the addition of NEt₃(30 μl, 3 equiv) at 0° C. The reaction mixture was then stirred for 24 h at RT. The reaction mixture was then concentrated under reduced pressure and the remaining residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give (2S,4R)—N2-(1-acetyl-1H-indol-3-yl)-N1-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-1,2-dicarboxamide. ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ 0.53 (s, br, 2H), 0.65 (d, J=5.6 Hz, 2H), 2.04-2.18 (m, 1H), 2.42 (s, 3H), 2.54-2.58 (m, 1H), 3.90 (ddd, J=22, 9.6, 3.2 Hz, 1H), 4.14 (dd, J=8.8, 12.4 Hz, 1H), 4.32 (dd, J=22.4, 6.0 Hz, 1H), 4.40-4.49 (m, 2H), 5.18 (d, J=17.2 Hz, 1H), 5.39 (d, J=17.2 Hz, 1H), 5.51 (d, J=52.8 Hz, 1H), 6.92-7.01 (m, 2H), 7.16-7.24 (m, 1H), 7.40-7.45 (m, 2H), 7.82-7.87 (m, 2H), 8.22 (s, 1H), 8.60 (t, J=5.6 Hz, 1H). ¹⁹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ −121.3, −176.1. LC (method 1): t_R=1.73 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₂₈H₂₇ClF₂N₄O₅, 573; found, 573.

Example 15. Synthesis of (2S,4R)—N2-(1-acetyl-1H-indol-3-yl)-N1-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-1,2-dicarboxamide (Compound F)

To a stirred solution of methyl 1H-indole-5-carboxylate (10 g, 1 equiv) in 200 mL DCM was added diethylaluminium chloride (1 M solution in hexane; 85.71 mL, 1.5 equiv) dropwise at 0° C. The mixture was stirred for 30 min and then a solution of acetyl chloride (6 mL, 1.5 equiv) in 150 mL DCM was added at 0° C. and stirred for 1 h at 0° C. A 5% aqueous citric acid solution was added at 0° C. and the reaction mixture was stirred for 15 min at RT. The precipitate was collected by filtration, washed with water, and dried. The residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give Methyl 3-acetyl-1H-indole-5-carboxylate.
To a suspension of methyl 3-acetyl-1H-indole-5-carboxylate (6 g, 1 equiv) and K₂CO₃(4.19 g, 1.1 equiv) in 50 mL acetonitrile was added tert-butyl 2-bromoacetate (4.42 mL, 1.1 equiv) dropwise at RT. The resulting mixture was then heated to reflux for 18 h. After cooling to RT, the mixture was diluted with DCM (100 mL), filtered through a pad of Celite; and the filtrate was concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (silica gel eluted with DCM/EtOAc) to give Methyl 3-acetyl-1-(2-(tert-butoxy)-2-oxoethyl)-1H-indole-5-carboxylate (12).
Methyl 3-acetyl-1-(2-(tert-butoxy)-2-oxoethyl)-1H-indole-5-carboxylate (3 g, 9.06 mmol) was taken up in 4 N HCl dioxane (50 mL) and the resulting reaction mixture was stirred at RT for 48 h. The solvent was then removed under reduced pressure and the remaining material 2-(3-Acetyl-5-(methoxycarbonyl)-1H-indol-1-yl)acetic acid was used directly in the next synthetic step.
2-(3-Acetyl-5-(methoxycarbonyl)-1H-indol-1-yl)acetic acid (2 g, 1 equiv) was dissolved in DMF (50 mL) and ⁱPr₂NEt (3.95 mL, 5 equiv) was added, followed by the sequential addition of (2S,4R)—N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide hydrochloride A (2.254 g, 1 equiv) and HATU (5.8 g, 2.1 equiv) slowly at 5° C. The reaction mixture was then stirred for 18 h at RT, diluted with 1 M citric acid solution (100 mL, containing 10 g NaCl), and extracted with DCM (50 mL×2). The organic layer was washed with an aqueous solution of NaHCO₃(40 mL), washed with water (40 mL), washed with brine (40 mL), dried over Na₂SO₄, and concentrated under reduced pressure. The remaining residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give methyl 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indole-5-carboxylate.
Methyl 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indole-5-carboxylate (3.86 g, 1 equiv) was taken up in a mixture of THF (20 mL) and methanol (20 mL), and then LiOH (1.832 g, 6 equiv) in water (20 mL) was added. The resulting reaction mixture was stirred at RT for 48 h. The solvent was then removed under reduced pressure and water (30 mL) was added. Acidification with 4 N HCl produced a white precipitate that was collected by filtration and dried in vacuo. This material 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indole-5-carboxylic acid (F) was used directly in the next synthetic step.

Example 16. Synthesis of cyclopropyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl)carbamate (Compound 187)

To a suspension of 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indole-5-carboxylic acid (155 mg, 0.3 mmol, 1 equiv) in THF (20 mL) was added Et₃N (38 μL, 1 equiv) and the resulting solution was stirred at RT under a nitrogen atmosphere for 15 min. DPPA (64 μL, 1 equiv) was added and the reaction was further stirred at RT under nitrogen for 18 h. The reaction mixture was concentrated under reduced pressure and the residue acyl azide intermediate was suspended in a mixture of toluene (20 mL) and THF (5 mL). This mixture was refluxed under nitrogen for 4 h and evaporated to dryness under reduce pressure. The remaining crude isocyanate (2S,4R)-1-(2-(3-Acetyl-5-isocyanato-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (G) was used directly in the next step.
Compound G was dissolved in a mixture of THF (7 mL), ACN (3 mL), and cyclopropanol (35 mg, 2 equiv), followed by the addition of NEt₃(76 μL, 2 equiv) at 0° C. The reaction mixture was then stirred for 24 h at RT. The reaction mixture was concentrated under reduced pressure and the remaining residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give product cyclopropyl (3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl)carbamate (187). ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ 0.68-0.72 (m, 4H), 2.00-2.17 (m, 1H), 2.40 (s, 3H), 2.49-2.56 (m, 1H), 3.89 (ddd, J=22.8, 9.6, 2.8 Hz, 1H), 4.05-4.14 (m, 1H), 4.32 (dd, J=20.8, 6.0 Hz, 1H), 4.42-4.49 (m, 2H), 5.12 (d, J=17.2 Hz, 1H), 5.33 (d, J=17.2 Hz, 1H), 5.50 (d, J=52.8 Hz, 1H), 6.97-7.01 (m, 1H), 7.15-7.45 (m, 4H), 8.18 (s, 1H), 8.31 (s, 1H), 8.59 (t, J=5.6 Hz, 1H), 9.49 (s, 1H). ¹⁹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ −121.8, −176.1. LC (method 1): t_R=1.78 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₂₈H₂₇ClF₂N₄O₅, 573; found, 573.

Example 17. Synthesis of N-(3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl)-3,3-difluoropiperidine-1-carboxamide (Compound 191)

Compound G from the previous reaction was dissolved in a mixture of THF (7 mL), ACN (3 mL), and 3,3-difluoropiperidine hydrochloride (51.81 mg, 1.1 equiv), followed by the addition of NEt₃(76 μL, 2 equiv) at 0° C. The reaction mixture was then stirred for 24 h at RT. The reaction mixture was concentrated under reduced pressure and the remaining residue was purified by flash column chromatography (silica gel eluted with DCM/CH₃OH) to give N-(3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-1H-indol-5-yl)-3,3-difluoropiperidine-1-carboxamide. ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ 1.69-1.76 (m, 2H), 2.01-2.17 (m 3H), 2.41 (s, 3H), 3.52 (t, J=5.2 Hz, 1H), 3.78-3.95 (m, 3H), 4.13 (dd, J=9.2, 12.4 Hz, 1H), 4.32 (dd, J=20.4, 6.0 Hz, 1H), 4.42-4.49 (m, 2H), 5.12 (d, J=17.2 Hz, 1H), 5.33 (d, J=17.2 Hz, 1H), 5.50 (d, J=52.8 Hz, 1H), 6.99-7.03 (m, 1H), 7.22-7.25 (m, 1H), 7.29-7.48 (m, 3H), 8.16 (s, 1H), 8.19 (d, J=2 Hz, 1H), 8.59 (t, J=6 Hz, 1H), 8.62 (s, 1H). ¹⁹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ −101.06, −121.25, −176.1. LC (method 1): t_R=1.82 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₃₀H₃₀ClF₄N₅O₄, 636; found, 636.

Example 18. Synthesis of (2S,4R)-1-(2-(3-acetyl-5-(pyrimidin-2-ylethynyl)-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (Compound 158)

1-(5-Bromo-1H-indol-3-yl)ethanone (2) was prepared from 5-bromoindole according to the procedure published by MacKay et al. (MacKay, J. A.; Bishop, R.; Rawal, V. H. Org. Lett. 2005, 7, 3421-3424.)
A mixture of 3.9 g (16.4 mmol) of 1-(5-bromo-1H-indol-3-yl)ethanone, 2.63 mL (18.02 mmol) of tert-butyl bromoacetate and 2.50 g (18.02 mmol) potassium carbonate in anhydrous acetonitrile (80 mL) was refluxed for 5 h. The reaction mixture was then cooled to RT and the solvent was removed under reduced pressure. The residue was taken in 1:1 mixture of CH₂Cl₂and water (100 mL: 100 mL). The two layers were separated and the organic layer was washed with water (2×100 mL). Finally, the organic layer was dried (Na₂SO₄) and concentrated. The resulting residue was stirred with 50 mL of heptane for 30 min., cooled in an ice bath and filtered, washing the solid with cold heptane (10 mL). This cream colored solid was dried under high vacuum to give 5.6 g of product tert-Butyl 2-(3-acetyl-5-bromo-1H-indol-1-yl)acetate.
4.5 g of tert-Butyl 2-(3-acetyl-5-bromo-1H-indol-1-yl)acetate was stirred in 1:1 mixture of CH₂Cl₂-TFA (90 mL) at RT for 4 h. The volatiles were removed under reduced pressure. The residue was dissolved in 45 mL of DMF. 4.16 g (13.4 mmol) of hydrochloride salt of 5 was added to this solution, followed by 11 mL of N,N-diisopropylethylamine (63.7 mmol). The reaction mixture was cooled in an ice bath and 5.82 g of (15.3 mmol) HATU was added. Following the addition of HATU, the cooling bath was removed and the reaction mixture was stirred overnight at RT. This reaction mixture was then poured in 450 mL of 1.0 M aq. citric acid solution. The separated product was isolated by filtration and the solid was washed thoroughly with water. This gray solid was dried under high vacuum to give 7.4 g of product (2S,4R)-1-(2-(3-Acetyl-5-bromo-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide.
A mixture of 1 g (1.8 mmol) of (2S,4R)-1-(2-(3-Acetyl-5-bromo-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide, 0.419 g (0.36 mmol) of tetrakis(triphenylphosphine)palladium(0), 0.138 g 0.72 mmol) of cuprous iodide in DMF (10 mL) was deoxygenated by bubbling argon in a pressure vessel. Triethylamine (2.53 mL, 18.1 mmol) and 2.56 mL (18.1 mmol) of ethynyltrimethylsilane were added under argon. The pressure vessel was capped and heated at 100° C. overnight. Then the reaction mixture was cooled to RT and the solvent was removed under reduced pressure. The residue was purified by column chromatography (silica gel, 0-2% MeOH in CH₂Cl₂) to give 0.56 g of (2S,4R)-1-(2-(3-Acetyl-5-((trimethylsilyl)ethynyl)-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide as a yellow solid.
To a solution of (2S,4R)-1-(2-(3-Acetyl-5-((trimethylsilyl)ethynyl)-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (0.5 g, 1 mmol) in THF (5 mL) was added 1.5 mL of 1.0 M tetrabutylammonium fluoride in THF at RT. The reaction mixture was stirred for 1 h. The solvent was removed under reduced pressure and the residue was purified by column chromatography (silica gel, 0-2% MeOH in CH₂Cl₂) to give 0.26 g of (2S,4R)-1-(2-(3-Acetyl-5-ethynyl-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide as a light yellow solid.
A mixture of 0.16 g (1.0 mmol) of 2-bromopyrimidine, 0.141 g (0.2 mmol) of PdCl₂(PPh₃)₂, 0.043 g (0.23 mmol) of cuprous iodide in DMF (4 mL) was deoxygenated by bubbling argon in a pressure vessel. Triethylamine (2.0 mL) and 0.250 g (0.5 mmol) of (2S,4R)-1-(2-(3-Acetyl-5-ethynyl-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide were added under argon. The pressure vessel was capped and heated at 100° C. overnight. Then the reaction mixture was cooled to RT and the solvent was removed under reduced pressure. The residue was purified by column chromatography (silica gel, 0-4.5% MeOH in CH₂Cl₂) to give 35 mg of the desired product (2S,4R)-1-(2-(3-Acetyl-5-(pyrimidin-2-ylethynyl)-1H-indol-1-yl)acetyl)-N-(3-chloro-2-fluorobenzyl)-4-fluoropyrrolidine-2-carboxamide (158) as a light red solid. ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ1.99-2.18 (m, 1H), 2.46 (s, 3H), 3.92 (ddd, J=24.4, 12.4, 2.8 Hz, 1H), 4.26 (dd, J=12.8, 20.8 Hz, 1H), 4.32 (dd, J=28.4, 6.0 Hz, 1H), 4.39-4.49 (m, 2H), 5.24 (d, J=17.2 Hz, 1H), 5.45 (d, J=17.2 Hz, 1H), 5.51 (d, J=52.8 Hz, 1H), 6.96 (t, J=7.2 Hz, 1H), 7.23 (t, J=6.4 Hz, 1H), 7.40-7.51 (m, 4H), 7.59 (d, J=8.4 Hz, 1H), 8.36 (s, 1H), 8.47 (d, J=0.8 Hz, 1H), 8.61 (t, J=5.6 Hz, 1H), 8.85 (d, J=4.8 Hz, 2H). ³¹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ-121.7, −176.1. LC (method 1): t_R=1.73 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₃₀H₂₄ClF₂N₅O₃, 575.9; found, 576.4.

Example 19. Synthesis of 3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl) carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-N-cyclopropylsulfonyl)-1H-indole-6-carboxamide (Compound 129)

Methyl 3-acetyl-1H-indole-6-carboxylate was prepared according to the procedure published by MacKay et al. (MacKay, J. A.; Bishop, R.; Rawal, V. H. Org. Lett. 2005, 7, 3421-3424.)
A mixture of 150 mg (0.68 mmol) of methyl 3-acetyl-1H-indole-6-carboxylate, 0.12 mL (0.76 mmol) of tert-butyl bromoacetate, and 249 mg (0.76 mmol) cesium carbonate in anhydrous acetonitrile (15 mL) was refluxed for 18 h. The reaction mixture was then cooled to RT and the solvent was removed under reduced pressure. The residue was taken in a 2:1 mixture of EtOAc and water (30 mL:15 mL). The two layers were separated and the organic layer was washed with brine (2×15 mL). Finally, the organic layer was dried (Na₂SO₄) and concentrated to obtain 283 mg of product methyl 3-acetyl-1-(2-tert-butoxy)-2-oxoethyl)-1H-indole-6-carboxylate (H) as a yellow solid.
100 mg of methyl 3-acetyl-1-(2-tert-butoxy)-2-oxoethyl)-1H-indole-6-carboxylate (0.3 mmol) was stirred in 4 N HCl in dioxane (15 mL) at RT for 18 h. The volatiles were removed under reduced pressure. The residue (intermediate I) was dissolved in 5 mL of DMF. To this solution was added 140 mg (0.36 mmol) of TFA salt of A, followed by 0.26 mL of N,N-diisopropylethylamine (1.5 mmol). Then 137 mg of (0.36 mmol) HATU was added and the reaction mixture was stirred overnight at RT. The reaction mixture was diluted with EtOAc (20 mL) and water (15 mL). The organic layer was separated, washed with brine (3×15 mL), dried (Na₂SO₄), concentrated in vacuo, and the residue was purified by column chromatography (silica gel, 0-10% MeOH in CH₂Cl₂) to give 128 mg of methyl-3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl-1H-indole-6-carboxylate as a yellow solid.
A mixture of 128 mg (0.24 mmol) of methyl-3-acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl-1H-indole-6-carboxylate in THF (5 mL) and 1 N LiOH (10 mL) was stirred at RT for 18 h. The solvent (THF) was removed under reduced pressure and the remaining water layer was washed with EtOAc (5 mL), acidified by 2 N HCl, and extracted with EtOAc (20 mL). The organic layer was washed with water, dried, and concentrated to obtain 126 mg of product 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl-1H-indole-6-carboxylic acid as a yellow solid.
To a stirred solution of 177 mg (0.34 mmol) of 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl-1H-indole-6-carboxylic acid in DMF (8 mL) was added 207 mg (1.71 mmol) of cyclopropanesulfonamide, 390 mg (1.03 mmol) of HATU, and 0.4 mL (2.4 mmol) DIEA. The reaction mixture was heated to 60° C. for 36 h. The reaction mixture was then cooled to RT and the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to give 63 mg of the desired product 3-Acetyl-1-(2-((2S,4R)-2-((3-chloro-2-fluorobenzyl)carbamoyl)-4-fluoropyrrolidin-1-yl)-2-oxoethyl)-N-cyclopropylsulfonyl)-1H-indole-6-carboxamide (129) as a white solid. ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ1.12 (m, 4H), 2.11 (m, 1H), 2.48 (s, 3H), 3.15 (m, 1H), 3.92 (m, 1H), 4.16 (m, 2H), 4.32 (m, 2H), 4.49 (m, 2H), 5.48 (m, 2H), 6.92 (t, 1H), 7.21 (t, 1H), 7.43 (t, 1H), 7.81 (d, 1H), 8.30 (d, 1H), 8.44 (s, 1H), 8.65 (t, 1H). ³¹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ-121.6, −176.0. LC (method 1): t_R=1.52 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₂₈H₂₇ClF₂N₄O₆S, 621.05; found, 621.00.

Example 20. Synthesis of (S)-3-acetyl-1-2(-(3-((3-chlorobenzyl)carbamoyl)morpholino)-2-oxoethyl)-1H-indole-6-carboxylic acid (Compound 160)

2-(3-Acetyl-6-(methoxycarbonyl)-1H-indol-1-yl)acetic acid-6-carboxylate I was prepared from H with HCl in dioxane according to the procedure in example 19.
273 mg (1.18 mmol) of (S)-4-Boc-morpholine-3-carboxylic acid was added to a solution of (3-chloro-2-flurophenyl)methanamine, 208 mg, 1.3 mmol) in DMF (10 mL), followed by addition of 0.41 mL of N,N-diisopropylethylamine (2.4 mmol), and 538 mg (1.4 mmol) of HATU. The reaction mixture was stirred overnight at RT. The reaction mixture was diluted with EtOAc (50 mL) and water (20 mL). The organic layer was separated, washed with brine (3×15 mL), dried (Na₂SO₄), concentrated in vacuo, and the remaining residue was purified by column chromatography (silica gel, 0-80% EtOAc in hexanes) to give 399 mg of a yellow oil. The coupling product obtained was deprotected with TFA in DCM at RT to form the TFA salt of (S)—N-(3-Chloro-2-fluorobenzyl)morpholine-3-carboxamide.
To a stirred solution of 261 mg (0.54 mmol) of (S)—N-(3-Chloro-2-fluorobenzyl)morpholine-3-carboxamide in DMF (7 mL) was added 155 mg (0.56 mmol) of 11 (2-(3-acetyl-6-(methoxycarbonyl)-1H-indol-1-yl)acetic acid-6-carboxylate), 244 mg (0.64 mmol) of HATU, and 0.28 mL (1.61 mmol) DIEA. The reaction mixture was stirred 18 h at room temperature. The reaction mixture was then diluted with EtOAc (45 mL) and water (25 mL). The organic layer was separated, washed with brine (3×25 mL), dried, and evaporated to dryness under reduced pressure. The residue was dissolved in THF (5 mL) and hydrolyzed with LiOH solution (1 N, 10 mL). The THF was removed under reduced pressure, and the remaining water layer was acidified with 1 N HCl and extracted with EtOAc (20 mL). The EtOAc layer was dried and concentrated in vacuo. The residue was purified by HPLC (C18, 10-100% acetonitrile in water) to give 57 mg of the desired product (S)-3-Acetyl-1-2(-(3-((3-chlorobenzyl)carbamoyl)morpholino)-2-oxoethyl)-1H-indole-6-carboxylic acid (160) as a white solid. ¹H NMR (400 MHz, DMSO-d₆, 300 K): (major rotamer) δ2.25 (s, 3H), 3.52 (m, 1H), 3.85 (dd, 2H), 4.21 (d, 1H), 4.34 (dd, 2H), 4.55 (m, 1H), 4.62 (m, 1H), 5.44 (m, 2H), 7.06 (t, 1H), 7.20 (t, 1H), 7.48 (t, 1H), 7.76 (d, 1H), 8.18 (d, 1H), 8.31 (s, 1H), 8.59 (t, 1H). ³¹F NMR (376 MHz, DMSO-d₆, 300 K): (major rotamer) δ-121.53. LC (method 1): t_R=1.46 min. LC/MS (EI) m/z: [M+H]⁺ calcd for C₂₅H₂₃ClF₂N₃O₆, 515.92; found, 516.00.

Example 21. Additional Compounds

Table 2 shows compounds of Examples 2 to 20 with biological data and shows additional compounds prepared by the methods shown in Examples 1 to 13. Routine changes in starting materials and reaction conditions, readily apparent to those of skill in the art, were used to make the particular compounds disclosed in Table 2. Three ***'s are used to denote compounds with an IC₅₀less than 1 micromolar, two **'s indicate compound with an IC₅₀between 1 micromolar and 10 micromolar, and one * denotes compounds with an IC₅₀greater than 10 micromolar, ND indicates “No Data.” A standard Factor D inhibition assay, such as the assay of Example 22, is used to determine the IC₅₀'s for the compounds.


				RT min
Cmp.			IC₅₀	(Method
No.	Structure	Name	(μM)	1 or 2)	M + 1

1		(2S,3aS,7aS)-1-(2-(3-acetyl-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)octahydro-1H- indole-2-carboxamide	***	2.33	510

2		(S)-N1-(1-carbamoyl-in-indol-3- yl)-N2-(3-chloro-2- fluorobenzyl)indoline-1,2- dicarboxamide	**	2.04	506

3		(2S,4R)-N2-(1-acetyl-1H-indol-3- yl)-N1-(3-chloro-2-fluorobenzyl)- 4-fluoropyrrolidine-1,2- dicarboxamide	*	2.07	475

4		(2S,4S)-N2-(1-acetyl-1H-indol-3- yl)-N1-(3-chloro-2-fluorobenzyl)- 4-fluoropyrrolidine-1,2- dicarboxamide	*	2.05	475

5		(2S,4R)-1-(2-(3-acetyl-6- (cyclopropylmethoxy)-1H-indol- 1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	2.14	544

6		(2S,4S)-N2-(1-acetyl-1H-indol-3- yl)-N1-benzyl-4-fluoropyrrolidine- 1,2-dicarboxamide	*	1.78	423

7		(2S,4R)-N1-(1-carbamoyl-1H- indol-3-yl)-N2-((3-chloro-2- fluorophenyl)sulfonyl)-4- fluoropyrrolidine-1,2- dicarboxamide	*	1.33	526

8		(2S,4R)-N1-(1-acetyl-1H-indol- 3-yl)-N2-((3-chloro-2- fluorophenyl)sulfonyl)-4- fluoropyrrolidine-1,2- dicarboxamide	*	1.67	525

9		(R)-N2-(1-acetyl-1H-indol-3-yl)- N1-(3-chloro-2-fluorobenzyl)- 4,4-difluoropyrrolidine-1,2- dicarboxamide	*	2.25	493

10		(R)-N2-(1-acetyl-1H-indol-3-yl)- N1-(3-chloro-2- fluorobenzyl)pyrrolidine-1,2- dicarboxamide	*	2.14	457

11		3-((2S,4R)-2-(5-(3-chlorophenyl)- 1H-imidazol-2-yl)-4- fluoropyrrolidine-1-carboxamido)- 1H-indole-1-carboxamide	**	1.49	467

12		2-(3-acetyl-1H-indol-1-yl)-1- ((2S,4R)-2-(5-(3-chlorophenyl)- 1H-imidazol-2-yl)-4- fluoropyrrolidin-1-yl)ethanone	*	1.46	465

13		3-((2S,4R)-2-(5-(6-bromopyridin-2- yl)-1H-imidazol-2-yl)-4- fluoropyrrolidine-1-carboxamido)- 1H-indole-1-carboxamide	**	1.34	512

14		2-(3-acetyl-1H-indol-1-yl)-1- ((2S,4R)-2-(5-(6-bromopyridin- 2-yl)-1H-imidazol-2-yl)-4- fluoropyrrolidin-1-yl)ethanone	**	1.43	512

15		3-((2S,4R)-2-(5-chloro-1H- benzo[d]imidazol-2-yl)-4- fluoropyrrolidine-1-carboxamido)- 1H-indole-1-carboxamide	**	1.42	441

16		2-(3-acetyl-1H-indol-1-yl)-1-((2S,4R)- 2-(5-chloro-1H-benzo[d]imidazol-2- yl)-4-fluoropyrrolidin-1-yl)ethanone	*	1.54	439

17		(R)-N2-(1-acetyl-1H-indol-3-yl)-N1- (2-fluoro-3- (trifluoromethoxy)phenyl)pyrrolidine- 1,2-dicarboxamide	*	2.30	493

18		(R)-N-(1-acetyl-1H-indol-3-yl)-1-(2- (3-chloro-2-fluorophenyl)acetyl)-4,4- difluoropyrrolidine-2-carboxamide	*	2.28	478

19		(2S,3R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-3- (cyclopropylmethoxy)pyrrolidine-2- carboxamide	***	2.12	526

20		(2S,4R)-N1-(1-acetyl-1H-indol-3-yl)- N2-(3-chloro-2-fluorobenzyl)-4-((7- methoxy-2-phenylquinolin-4- yl)oxy)pyrrolidine-1,2-dicarboxamide	*	Method 2, 6.93	706

21		1,3-bis(1-acetyl-1H-indol-3-yl)urea	*	1.98	375

22		(2S,3S,4S)-1-(2-(3-acetyl-6-hydroxy- 1H-indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-3- (cyclopropylmethoxy)-4- fluoropyrrolidine-2-carboxamide	***	1.91	560

23		(1R,3S,5R)-N-(3-chloro-2- fluorobenzyl)-2-(2-(3- (cyclopropanecarbonyl)-1H-indol-1- yl)acetyl)-2-azabicyclo[3.1.0]hexane- 3-carboxamide	***	2.07	494

24		(1R,2R)-N1-(1-carbamoyl-1H-indol- 3-yl)-N2-(3-chloro-2- fluorobenzyl)cyclopentane-1,2- dicarboxamide	**	1.87	457

25		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-(cyclopropanecarbonyl)-1H- indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	2.02	500

26	mix	(2S)-1-(2-(3-acetyl-6-hydroxy-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-3- (cyclopropylmethoxy)-4- fluoropyrrolidine-2-carboxamide and (2R)-1-(2-(3-acetyl-6-hydroxy-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4- (cyclopropylmethoxy)-3- fluoropyrrolidine-2-carboxamide	***	1.82 and 1.86	560

27		(1R,2R)-N1-(1-carbamoylindolin-3- yl)-N2-(3-chloro-2- fluorobenzyl)cyclopentane-1,2- dicarboxamide	*	1.35	459

28		2-(3-acetyl-1H-indol-1-yl)-1- ((2S,4R)-4-fluoro-2-((4-(4- fluorophenyl)-1H-1,2,3-triazol-1- yl)methyl)pyrrolidin-1-yl)ethanone	*	1.72	464

29		3-((2S,4R)-4-fluoro-2-((4-(4- fluorophenyl)-1H-1,2,3-triazol-1- yl)methyl)pyrrolidine-1- carboxamido)-1H-indole-1- carboxamide	*	1.60	466

30		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-((7-methoxy-2- phenylquinolin-4-yl)oxy)pyrrolidine- 2-carboxamide	*	Method 2, 6.18	705

31		3-Acetyl-1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl trifluoromethanesulfonate	***	2.24	622

32		(2S,4R)-2-((4-(3-chloro-4- fluorophenyl)-1H-1,2,3-triazol-1- yl)methyl)-4-fluoropyrrolidine-1- carboxamide	*	1.15	342

33		N1-(1-carbamoyl-1H-indol-3-yl)-N2- (3-chloro-2-fluorobenzyl)cyclopent- 1-ene-1,2-dicarboxamide	*	1.97	455

34		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-(1-(cyanoimino)ethyl)-1H- indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	**	1.48	498

35		(1R,3S,5R)-2-(2-(3-acetyl-6- (cyclopropylmethoxy)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-2- azabicyclo[3.1.0]hexane-3- carboxamide	***	1.46	538

36		(2S,4R)-1-(2-(3-acetyl-6- (cyclopropylmethoxy)-1H-indol-1- yl)acetyl)-4-fluoro-N-(2-fluoro-3- (trifluoromethoxy)phenyl)pyrrolidine- 2-carboxamide	***	1.66	580

37		2-(3-acetyl-1H-indol-1-yl)-1- ((2S,4R)-4-fluoro-2-(isoindoline-2- carbonyl)pyrrolidin-1-yl)ethanone	*	0.95	434

38		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoro-N- methylpyrrolidine-2-carboxamide	*	1.35	488

39		(2S,4R)-1-(2-(3-acetyl-6-(2- cyclopropylethoxy)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorophenyl)-4-fluoropyrrolidine-2- carboxamide	***	1.70	558

40		(2S,3aS,7aS)-1-(2-(3-acetyl-6- hydroxy-1H-indol-1-yl)acetyl)-N-(3- chloro-2-fluorobenzyl)octahydro-1H- indole-2-carboxamide	***	1.30	526

41		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-cyano-1H-indol-1-yl)acetyl)- 4-fluoropyrrolidine-2-carboxamide	***	1.38	457

42		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(4-oxo-3,4-dihydro-1H- carbazol-9(2H)-yl)acetyl)pyrrolidine- 2-carboxamide	***	1.18	500

43		tert-butyl ((S)-1-((2S,4R)-2-((3- chloro-2-fluorobenzyl(carbamoyl)-4- fluoropyrrolidin-1-yl)-3-(1H-indol-3- yl)-1-oxopropan-2-yl(carbamate	*	1.66	561

44		(2S,4R)-1-((S)-2-amino-3-(1H-indol- 3-yl)propanoyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	*	0.40	461

45		2-(3-acetyl-6-hydroxy-1H-indol-1- yl)-1-((2S,4R)-2-(5-(6-bromopyridin- 2-yl)-1H-imidazol-2-yl)-4- fluoropyrrolidin-1-yl)ethanone	**	0.93	526

46		(S)-7-(2-(3-acetyl-6-hydroxy-1H- indol-1-yl)acetyl)-N-(2-fluoro-3- (trifluoromethoxy)phenyl)-1,4-dioxa- 7-azaspiro[4.4]nonane-8- carboxamide	**	1.21	566

47			**	2.25	710

48		diethyl (3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl)phosphonate	***	1.83	610

49		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(2,3-dihydro-1H-inden- 1-yl)-4-fluoropyrrolidine-2- carboxamide	**	1.06	448

50		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(2,3-dihydro-1H-inden- 2-yl)-4-fluoropyrrolidine-2- carboxamide	***	1.05	448

51		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)acetyl)-N-(4-chloro-2,3-dihydro- 1H-inden-1-yl)-4-fluoropyrrolidine- 2-carboxamide	**	1.15	482

52		ethyl hydrogen (3-acetyl-1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl)phosphonate	***	1.20	582

53		(2S,4R)-1-(2-(3-acetyl-1H- pyrazolo[3,4-c]pyridin-1-yl)acetyl)- 4-fluoro-N-(3-((trifluoro- methyl)thio)phenyl)pyrrolidine-2- carboxamide	***	1.16	511

54		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(3-nitro-1H-indol-1- yl)acetyl)pyrrolidine-2-carboxamide	***	1.37	477

55		(2S,4R)-1-(2-(3-acetamido-1H-indol- 1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.01	489

56		(2S,4R)-1-(2-(3-acetyl-6-hydroxy- 1H-indol-1-yl)acetyl)-4-fluoro-N-(3- ((trifluoromethyl)thio)phen- yl)pyrrolidine-2-carboxamide	***	1.09	524

57		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(1,1-dioxido-2H- benzo[e][1,2,4]thiadiazin-3-yl)acetyl)- 4-fluoropyrrolidine-2-carboxamide	**	0.86	497

58		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(pyridin-3-yl)thiazole- 4-carbonyl)pyrrolidine-2- carboxamide	*	0.77	463

59		(2S,4R)-1-(6-acetamidonicotinoyl)- N-(3-chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	*	0.55	437

60		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(3-iodo-1H- pyrazolo[3,4-c]pyridin-1- yl)acetyl)pyrrolidine-2-carboxamide	***	1.00	562

61		diethyl (1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-pyrazolo[3,4-c]pyridin-3- yl)phosphonate	**	0.82	572

62		(2S,4R)-N2-(3-chloro-2- fluorobenzyl)-N1-(1- (cyclopropanecarbonyl)-1H-indol-3- yl)-4-fluoropyrrolidine-1,2- dicarboxamide	***	1.28	501

63		(2S,4R)-1-(2-(3-acetyl-1H-indol-1- yl)propanoyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	*	1.17	488

64		(2S,4R)-1-(2-(3-acetyl-1H-indol-1-yl)- 2-methylpropanoyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	*	1.96	502

65		(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-pyrazolo[3,4-c]pyridin-3- yl)phosphonic acid	*	0.72	514

66		diethyl (((3-acetyl-1-(2-((2S,4R)-2- ((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6- yl)oxy)methyl)phosphonate	***	1.84	640

67		(2S,4R)-1-(2-(4-acetylnaphthalen-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	**	2.04	485

68		(3-acetyl-1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl(carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl)phosphonic acid	***	1.07	554

69		diethyl (3-((2S,4R)-1-(2-(3-acetyl- 1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamido)-5- chlorophenyl)phosphonate	**	1.89	578

70		ethyl hydrogen (1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-pyrazolo[3,4-c]pyridin-3- yl)phosphonate	**	0.86	542

71		(((3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)- 4-fluoropyrrolidin-1-yl)-2- oxoethyl)-1H-indol-6- yl)oxy)methyl)phosphonic acid	***	1.03	584

72		diethyl 3-(((3R,5S)-1-(2-(3-acetyl- 1H-indol-1-yl)acetyl)-3- fluoropyrrolidine-5- carboxamido)methyl)-5-chloro-4- fluorophenylphosphonate	***	1.82	610

73		Di-isopropoxycarboxymethyl 1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	2.31	786

74		(2S,3aR,6aR)-N-(3-chloro-2- fluorobenzyl)-1-(2-(3-acetyl-6- hydroxy-1H-indol-1-yl)acetyl)- octahydrocyclopenta[b]pyrrole-2- carboxamide	***	1.88	512

75		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(3- (aminomethyl)pyrrolidine-1- carbonyl)-1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.02	600

76		diethyl 1-(2-((2S,3aR,6aR)-2-((3- chloro-2-fluorobenzyl)carbamoyl)- hexahydrocyclopenta[b]pyrrol-1(2H)- yl)-2-oxoethyl)-3-acetyl-1H-indol-6- ylphosphonate	***	2.13	632

77		3-(((3R,5S)-1-(2-(3-acetyl-1H-indol- 1-yl)acetyl)-3-fluoropyrrolidine-5- carboxamido)methyl)-5-chloro-4- fluorophenylphosphonic acid	***	0.98	554

78		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl 2-(3-acetyl-5- hydroxy-1H-indol-1-yl)acetate	***	1.77	705

79		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl trifluoromethanesulfonate	***	2.37	622

80		((1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yl)(ethoxy)phosphoryloxy)methyl isopropyl carbonate	***	2.08	698

81		1-(2-((2S,3aR,6aR)-2-((3-chloro-2- fluorobenzyl)carbamoyl)- hexahydrocyclopenta[b]pyrrol-1 (2H)-yl)-2-oxoethyl)-3-acetyl-1H- indol-6-ylphosphonic acid	***	1.43	576

82		diethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-ylphosphonate	***	1.74	610

83		ethyl hydrogen 1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-ylphosphonate	*	1.19	582

84		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-ylphosphonic acid	***	1.04	554

85		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl thiazol-4- ylmethylcarbamate	***	1.64	630

86		ethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-6- (diethoxyphosphoryl)-1H-indazole-3- carboxylate	*	2.15	641

87		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-6- (diethoxyphosphoryl)-1H-indazole- 3-carboxylic acid	*	1.65	613

88		(2S,4R)-1-(2-(3-acetyl-6-hydroxy- 1H-indol-1-yl)acetyl)-N-((1R)-1- (cyclopropylsulfonylcarbamoyl)-2- vinylcyclopropyl)-4- fluoropyrrolidine-2-carboxamide	**	1.16	561

89		methyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-6- (diethoxyphosphoryl)-1H-indole-3- carboxylate	***	2.03	626

90		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- (methoxycarbonyl)-1H-indol-6- ylphosphonic acid	***	1.22	570

91		diethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- carbamoyl-1H-indol-6-ylphosphonate	***	1.54	611

92		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- carbamoyl-1H-indol-6-ylphosphonic acid	***	0.72	555

93		diethyl (1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5- yloxy)methylphosphonate	***	1.79	640

94		(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5- yloxy)methylphosphonic acid	***	1.02	584

95		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-N-(methylsulfonyl)-1H-indole- 6-carboxamide	***	1.60	595

96		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-yl thiazol-4- ylmethylcarbamate	***	1.68	630

97		diethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- carbamoyl-1H-indazol-6- ylphosphonate	***	1.59	612

98		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- carbamoyl-1H-indazol-6- ylphosphonic acid	***	0.70	556

99		bis(2,2,2-trifluoroethyl) 1-(2-((2S,4R)- 2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	2.26	718

100		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-yl (2H-tetrazol-5- yl)methylcarbamate	***	1.42	615

101		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-yl 2-(2H-tetrazol- 5-yl)ethylcarbamate	***	1.45	629

102		2-((1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)carbonyl)ethylphosphonic acid	***	1.16	641

103		4-(((1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)carbonyl)methyl)phenylboronic acid	***	1.65	667

104		dibutyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	2.47	666

105		butyl hydrogen 1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	1.49	610

106		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-ethynyl-1H-indol-1- yl)acetyl)-4-fluoropyrrolidine-2- carboxamide	***	1.97	498

107		3-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-yl)propiolic acid	***	1.52	542

108		tert-butyl 1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylcarbamate	***	1.90	589

109		Di(isopropoxycarboxymethyl)(1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methylphosphonate	***	2.16	816

110		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl cyclopropylcarbamate	***	1.73	573

111		(S)-tert-butyl 3-(1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indole-6- carboxamido)pyrrolidine-1- carboxylate	***	1.92	686

112		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-N-((S)-pyrrolidin-3-yl)-1H- indole-6-carboxamide	***	1.10	586

113		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6- (trifluoromethylsulfonamido)-1H- indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.54	621

114		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl oxazol-4- ylmethylcarbamate	***	1.55	614

115		isopropoxycarboxymethyl hydrogen 1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	1.34	670

116		isopropoxycarboxymethyl hydrogen (1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methylphosphonate	***	1.38	700

117		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(2- (trimethylsilyl)ethynyl)-1H-indol-1- yl)acetyl)-4-fluoropyrrolidine-2- carboxamide	***	2.71	570

118		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl (1H-tetrazol-5- yl)methylcarbamate	***	1.37	615

119		ethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yl(phenyl)phosphinate	***	1.97	642

120		ethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yl(ethyl)phosphinate	***	1.64	594

121		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yl(phenyl)phosphinic acid	***	1.49	614

122		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 3-acetyl-N-((S)-1-acetylpyrrolidin- 3-yl)-1H-indole-6-carboxamide	***	1.30	628

123		dipivaloyloxy 1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	2.41	782

124		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yl(ethyl)phosphinic acid	***	1.24	566

125		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(2- (methylsulfinyl)acetyl)-1H-indol-1- yl)acetyl)-4-fluoropyrrolidine-2- carboxamide	***	1.35	578

126		pivaloyloxymethyl hydrogen 1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	1.14	668

127		ethyl (1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methyl(ethyl)phosphinate	***	1.40	624

128		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(4,4-dioxo-4-thio-2- (quinazolin-1(4H)- yl)acetyl)pyrrolidine-2-carboxamide	*	1.25	497

129		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-N-(cyclopropylsulfonyl)-1H- indole-6-carboxamide	***	1.52	621

130		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- f]uoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-N-(pyrrolidin-3-ylmethyl)-1H- indole-6-carboxamide	***	0.70	600

131		tert-butyl 3-((1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indole-6- carboxamido)methyl)pyrrolidine-1- carboxylate	***	1.77	700

132		methyl 3-(1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-yl)propiolate	***	1.18	556

133		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(tert- butylaminosulfonylmethoxy)-1H- indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.83	639

134		(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methyl(ethyl)phosphinic acid	***	1.18	596

135		2,2,2-trifluoroethyl hydrogen 1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6-ylphosphonate	***	1.08	636

136		methyl 1-(1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indole-6- carbonyl)pyrrolidine-3-carboxylate	***	1.28	629

137		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indole-6- carbonyl)pyrrolidine-3-carboxylic acid	***	1.14	615

138		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl morpholine-4- carboxylate	***	1.39	603

139		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl pyrimidin-2- ylmethylcarbamate	***	1.10	625

140		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6- (aminosulfonylmethoxy)-1H-indol-1- yl(acetyl)-4-fluoropyrrolidine-2- carboxamide	***	1.19	583

141		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-N-(phenylsulfonyl)-1H-indole- 6-carboxamide	***	1.71	657

142		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl 2-(2H-tetrazol- 5-yl)ethylcarbamate	***	1.14	629

143		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl 2-(1H-imidazol- 4-yl)ethylcarbamate	***	0.95	627

144		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(2-(4-amino-2-oxo- 1,2-dihydropyrimidin-5-yl)ethynyl)- 1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.02	607

145		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl piperazine-1- carboxylate	***	1.11	602

146		diethyl 1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-5-fluoro-1H-indol-6- ylphosphonate	***	1.41	628

147		3-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl)propiolic acid	***	1.26	542

148		diethyl (1-(2-((2S,4R)-2-(((R)-1-(3- chloro-2- fluorophenyl)ethyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methylphosphonate	***	1.67	654

149		(1-(2-((2S,4R)-2-(((R)-1-(3-chloro-2- fluorophenyl)ethyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methylphosphonic acid	***	0.79	598

150		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-5-(2-(4-amino-2-oxo- 1,2-dihydropyrimidin-5-yl)ethynyl)- 1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.17	607

151		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-5-fluoro-1H-indol-6- ylphosphonic acid	***	0.93	572

152		3-(hexadecyloxy(propyl hydrogen (1- (2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-6- yloxy)methylphosphonate	***	3.31	866

153		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl)-3- cyclopropylurea	***	1.24	572

154		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indole-5-carbonyl)-1,2,3,6- tetrahydropyridin-4-ylboronic acid	***	0.93	627

155		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl)-3-((2H- tetrazol-5-yl)methyl)urea	***	1.01	614

156		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-5-(3- (ethylsulfonamido)-3-oxoprop-1- ynyl)-1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.73	633

157		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-5-(2-(pyrimidin-2- yl)ethynyl)-1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.74	576

158		(2S,3aR,7aR)-N-(3-chloro-2- fluorobenzyl)-1-(2-(3-acetyl-6- hydroxy-1H-indol-1-yl)acetyl)- octahydro-1H-indole-2-carboxamide	**	1.93	526

159		(S)-1-(2-(3-((3-chloro-2- fluorobenzyl)carbamoyl)morpholino)- 2-oxoethyl)-3-acetyl-1H-indole-6- carboxylic acid	***	1.46	516

160		tert-butyl 9-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl(carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 3,4-dihydro-1H-pyrido[3,4-b]indole- 2(9H)-carboxylate	*	2.71	587

161		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(1,2,3,4- tetrahydropyrido[3,4-b]indol-9- yl(acetyl)pyrrolidine-2-carboxamide	*	1.38	487

162		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(2-(methylsulfonyl)- 1,2,3,4-tetrahydropyrido[3,4-b]indol- 9-yl)acetyl)pyrrolidine-2- carboxamide	**	2.06	565

163		ethyl 3-(9-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 3,4-dihydro-1H-pyrido[3,4-b]indol- 2(9H)-yl)-3-oxopropanoate	*	2.16	601

164		1-(1-(2-((1R,3S,5R)-3-((3-chloro-2- fluorobenzyl)carbamoyl)-2-aza- bicyclo[3.1.0]hexan-2-yl)-2-oxoethyl)- 3-acetyl-1H-indol-5-yl)-3- cyclopropylurea	***	1.52	566

165		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-6-(3- (ethylsulfonamido)-3-oxoprop-1- ynyl)-1H-indol-1-yl)acetyl)-4- fluoropyrrolidine-2-carboxamide	***	1.73	633

166		(2S,4R)-1-(2-(1-acetyl-1H-indol-3- yl)-2-oxoacetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	*	2.31	488

167		(2S,4R)-1-(2-(3-acetyl-5-(3-(1- methylcyclopropyl)ureido)-1H-indol- 1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.66	586

168		(1R,3S,5R)-2-(2-(3-acetyl-5-(3- cyclopropylureido)-1H-indol-1- yl)acetyl)-N-((R)-1-(3-chloro-2- fluorophenyl)ethyl)-2- azabicyclo[3.1.0]hexane-3- carboxamide	***	1.74	580

169		(2S,4R)-1-(2-(3-acetyl-5-(3- isopropylureido)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.65	574

170		3-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- (2,2,2-trifluoroacetyl)-1H-indol-6- yl)propiolic acid	***	2.11	596

171		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- (2,2,2-trifluoroacetyl)-1H-indol-6- ylphosphonic acid	***	1.54	608

172		4-(9-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 3,4-dihydro-1H-pyrido[3,4-b]indol- 2(9H)-yl)-4-oxobutanoic acid	*	1.88	587

173		Di(pivaloylmethyl)(1-(2-((2S,4R)-2- ((3-chloro-2-fluorobenzyl)carbamoyl)- 4-fluoropyrrolidin-1-yl)-2-oxoethyl)- 3-acetyl-1H-indol-6- yloxy)methylphosphonate	***	2.58	812

174		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl)-3-(isoxazol-3- ylmethyl)urea	***	1.49	613

175		(2S,4R)-1-(2-(3-acetyl-5-(3- (pyrimidin-2-ylmethyl)ureido)-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.35	624

176		N-(3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)pyrrolidine-1- carboxamide	***	1.61	586

177		(2S,4R)-1-(2-(3-acetyl-5-((2-fluoro-3- (trifluoromethoxy)phenyl)ethynyl)- 1H-indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	**	2.83	676

178		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-5-(2-(5- hydroxypyrimidin-2-yl)ethynyl)-1H- indol-1-yl)acetyl)-4-fluoropyrrolidine- 2-carboxamide	***	1.44	592

179		(2S,4R)-1-(2-(3-acetyl-5- (phenylethynyl)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	2.54	574

180		(2S,4R)-1-(2-(3-acetyl-5-(3- cyclopropylureido)-1H-indol-1- yl)acetyl)-4-fluoro-N-(2-fluoro-3- (trifluoromethoxy)phenyl)pyrrolidine- 2-carboxamide	***	1.80	608

181		(1R,3S,5R)-2-(2-(3-acetyl-5-(3- cyclopropylureido)-1H-indol-1- yl)acetyl)-N-(2-fluoro-3- (trifluoromethoxy)phenyl)-2- azabicyclo[3.1.0]hexane-3- carboxamide	***	1.88	602

182		(((3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2- fluorophenylsulfonamido)methyl)- 4-fluoropyrrolidin-1-yl)-2- oxoethyl)-1H-indol-6- yl)oxy)methyl)phosphonic acid	***	1.11	620

183		(1R,3S,5R)-2-(2-(3-acetyl-5- (pyrrolidine-1-carboxamido)-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-2- azabicyclo[3.1.0]hexane-3- carboxamide	***	1.72	581

184		N-(3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)-3,3- difluoropyrrolidine-1-carboxamide	***	1.77	622

185		diphenyl (3-acetyl-1-(2-((2S,4R)-2- ((3-chloro-2-fluorobenzyl)carbamoyl)- 4-fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl)phosphonate	***	2.44	707

186		cyclopropyl (3-acetyl-1-(2-((2S,4R)-2- ((3-chloro-2-fluorobenzyl)carbamoyl)- 4-fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)carbamate	***	1.78	573

187		cyclopropylmethyl (3-acetyl-1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)carbamate	***	1.96	587

188		1-(2-((2S,4R)-2-((3-ethynyl-2- fluorophenyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-pyrazolo[3,4-c]pyridine-3- carboxamide	***	0.91	453

189		(2S,4R)-1-(2-(3-acetyl-5-(pyrimidin- 5-ylethynyl)-1H-indol-1-yl)acetyl)- N-(3-chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	1.84	576

190		N-(3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)-3,3- difluoropiperidine-1-carboxamide	***	1.82	636

191		(2S,4R)-1-(2-(3-acetyl-5-(pyridin-4- ylethynyl)-1H-indol-1-yl)acetyl)-N- (3-chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	1.52	575

192		(((((3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl(carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-6-yl)oxy)meth- yl)phosphoryl)bis(oxy))bis(2-methyl- propane-1,1-diyl)dipropionate	***	2.79	840

193		N-(3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((2-fluoro-3-(trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-1H-indol-5-yl)-3,3- difluoropiperidine-1-carboxamide	***	2.06	672

194		(2S,4R)-1-(2-(3-acetyl-5-(isothiazol-4- ylethynyl)-1H-indol-1-yl)acetyl)-N-(3- chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	2.18	581

195		(2S,4R)-1-(2-(3-acetyl-5-(pyridin-2- ylethynyl)-1H-indol-1-yl)acetyl)-N- (3-chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	1.86	575

196		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (2-(pyrimidin-2-yl)ethynyl)-1H- indazole-3-carboxamide	***	1.52	578

197		N-(3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((2-fluoro-3-(2-chlorophenyl)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-1H-indol-5-yl)-3,3- difluoropiperidine-1-carboxamide	***	2.25	698

198			***	2.20	810

199		(1R,3S,5R)-2-(2-(3-acetyl-5-(2- (pyrimidin-2-yl)ethynyl)-1H-indol-1- yl)acetyl)-N-(6-bromopyridin-2-yl)- 2-aza-bicyclo[3.1.0]hexane-3- carboxamide	***	1.84	583

200		1-(2-((2S,4R)-4-fluoro-2-((2-fluoro- 3-(2-chlorophenyl)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-5-(2-(pyrimidin-2- yl)ethynyl)-1H-indazole-3- carboxamide	***	1.99	640

201		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (2-(pyridin-2-yl)ethynyl)-1H- indazole-3-carboxamide	***	1.66	577

202		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 1-(2-(3-acetyl-5-(2-(3-cyano-1H- pyrazol-4-yl)ethynyl)-1H-indol-1- yl)acetyl)-4-fluoropyrrolidine-2- carboxamide	***	1.84	589

203		1-(1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-3- acetyl-1H-indol-5-yl)-3-(isoxazol-3- yl)urea	***	1.60	599

204		1-(2-((2S,4R)-2-((3-chloro-2- fluorophenyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H-indazole- 3-carboxamide	***	1.56	564

205		N-(3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((6-(trifluoromethyl)pyridin-2- yl)carbamoyl(pyrrolidin-1-yl)-2- oxoethyl)-1H-indol-5-yl)-3,3- difluoropiperidine-1-carboxamide	***	1.89	639

206		(2S,4R)-1-(2-(3-acetyl-5-(3-(2,2,2- trifluoroethyl)ureido)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.72	614

207		N-(3-acetyl-1-(2-((2S,4R)-2-((3- chloro-2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indol-5-yl)-5H- pyrrolo[3,4-d]pyrimidine-6(7H)- carboxamide	***	1.38	636

208		(2S,4R)-1-(2-(3-acetyl-5-(3- (pyrimidin-5-yl)ureido)-1H-indol-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.4	610

209		(2S,4R)-1-(2-(3-acetyl-5-(pyrazin-2- ylethynyl)-1H-indol-1-yl)acetyl)-N- (3-chloro-2-fluorobenzyl)-4- fluoropyrrolidine-2-carboxamide	***	1.86	576

210		(2S,4R)-1-(2-(3-(1H-pyrazol-4-yl)- 1H-pyrazolo[3,4-c]pyridin-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.0	500

211		1-(2-((1R,3S,5R)-3-((2′-chloro-2- fluoro-[1,1′-biphenyl]-3- yl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	2.1	634

212		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- N6-(cyclopropylsulfonyl)-1H- indazole-3,6-dicarboxamide	***	2.0	685

213		(((3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((3-fluoro-4-(trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-1H-indol-6- yl)oxy)methyl)phosphonic acid	***	1.46	620

214		1-(2-((2S,4R)-2-((3-(3-chloropyridin- 2-yl)-2-fluorophenyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H-indazole- 3-carboxamide	***	1.54	641

215		(2S,4R)-N-(3-chloro-2-fluorobenzyl)- 4-fluoro-1-(2-(3-(1-fluorovinyl)-1H- pyrazolo[3,4-c]pyridin-1- yl)acetyl)pyrrolidine-2-carboxamide	***	1.61	478

216		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (3,3-difluoropiperidine-1- carboxamido)-1H-indazole-3- carboxamide	***	2.09	700

217		5-((1H-pyrazol-4-yl)ethynyl)-1-(2- ((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indazole-3-carboxamide	***	1.49	566

218		1-(2-((2S,4R)-2-((3′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H-indazole- 3-carboxamide	***	2.1	640

219		1-(2-((2S,4R)-4-fluoro-2-((2-fluoro- 3-(trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-5-(3-(isoxazol-3- yl)ureido)-1H-indazole-3- carboxamide	***	1.71	637

220		5-(3,3-difluoropiperidine-1- carboxamido)-1-(2-((2S,4R)-4- fluoro-2-((2-fluoro-3- (trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-1H-indazole-3- carboxamide	***	1.90	674

221		(2S,4R)-1-(2-(3-acetyl-5-(2- azabicyclo[2.2.1]heptane-2-carbonyl)- 1H-indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.76	597

222		(2S,4R)-1-(2-(3-acetyl-5-(3,3- difluoropiperidine-1-carbonyl)-1H- indol-1-yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-4-fluoropyrrolidine-2- carboxamide	***	1.83	621

223		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H- pyrazolo[3,4-c]pyridine-3- carboxamide	***	1.32	579

224		1-(2-((2S,4R)-4-fluoro-2-((6- (trifluoromethyl)pyridin-2- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	1.61	581

225		5-(3,3-difluoropiperidine-1- carboxamido)-1-(2-((1R,3S,5R)- 3-((2-fluoro-3-(trifluoro- methoxy)phenyl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-1H-indazole-3- carboxamide	***	1.97	668

226		3-acetyl-1-(2-((2S,4R)-2-((3-chloro- 2-fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- N-cyclopropyl-1H-indole-5- carboxamide	***	1.54	557

227		1-(2-((1R,3S,5R)-3-((2-fluoro-3- (trifluoromethoxy)phenyl)carbamoyl)- 2-azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	1.87	608

228		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- ((5-fluoropyridin-2-yl)ethynyl)-1H- indazole-3-carboxamide	***	1.91	595

229		(2S,4R)-1-(2-(3-(1H-imidazol-5-yl)- 1H-pyrazolo[3,4-c]pyridin-1- yl)acetyl)-N-(3-chloro-2- fluorobenzyl)-fluoropyrrolidine-2- carboxamide	***	0.86	500

230		5-((6-aminopyridin-2-yl)ethynyl)-1- (2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)- 1H-indazole-3-carboxamide	***	1.27	592

231		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- ((6-fluoropyridin-2-yl)ethynyl)-1H- indazole-3-carboxamide	***	1.96	595

232		(((3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((3- phenoxyphenyl)carbamoyl)pyrrolidin- 1-yl)-2-oxoethyl)-1H-indol-6- yl)oxy)methyl)phosphonic acid	**	1.51	610

233		(((3-acetyl-1-(2-((2S,4R)-4-fluoro-2- ((2-fluoro-3-(trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-1H-indol-6- yl)oxy)methyl)phosphonic acid	***	1.34	620

234		1-(2-((2S,4R)-4-fluoro-2-((2-fluoro- 3-(trifluoromethoxy)phen- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	1.77	614

235		1-(2-((2S,4R)-2-((6-bromopyridin-2- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(pyrimidin-2- ylethynyl)-1H-indazole-3- carboxamide	***	1.48	591

236		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (1H-pyrazol-4-yl)-1H-indazole-3- carboxamide	***	1.79	604

237		1-(2-((1R,3S,5R)-3-((2′-chloro-2- fluoro-[1,1′-biphenyl]-3- yl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-(3,3-difluoropiperidine- 1-carboxamido)-1H-indazole-3- carboxamide	***	2.17	694

238		1-(2-((2S,4R)-2-((3-(3-chloropyridin- 2-yl)-2-fluorophenyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (3,3-difluoropiperidine-1- carboxamido)-1H-indazole-3- carboxamide	***	1.65	701

239		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-5-yl)-1H-indazole-3- carboxamide	***	1.82	616

240		1-(2-((2S,4R)-2-((3-chloro-2- fluorobenzyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- ((5-fluoropyrimidin-2-yl)ethynyl)- 1H-indazole-3-carboxamide	***	1.73	596

241		1-(2-((1R,3S,5R)-3-((6-bromopyridin- 2-yl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	1.6	585

242		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (3,3-difluoropyrrolidine-1- carboxamido)-1H-indazole-3- carboxamide	***	2.03	686

243		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (3,3-difluoropyrrolidine-1- carboxamido)-1H-indazole-3- carboxamide	***	2.11	700

244		(2S,4R)-1-(2-(3-acetyl-5-(pyrimidin- 2-ylethynyl)-1H-indol-1-yl)acetyl)-N- (6-bromopyridin-2-yl)-4- fluoropyrrolidine-2-carboxamide	***	1.70	589

245		1-(2-((1R,3S,5R)-3-((6-bromopyridin- 2-yl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-((5-fluoropyrimidin-2- yl)ethynyl)-1H-indazole-3- carboxamide	***	1.81	603

246		1-(2-((2S,4R)-2-((3,3- dimethylcyclohexyl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H-indazole- 3-carboxamide	***	1.69	546

247		1-(2-((2S,4R)-4-fluoro-2-((2-fluoro-3- methylbut-2-en-1- yl)carbamoyl)pyrrolidin-1-yl)-2- oxoethyl)-5-(pyrimidin-2-ylethynyl)- 1H-indazole-3-carboxamide	***	1.31	522

248		1-(2-((2S,4R)-2-((6-bromopyridin-2- yl)carbamoyl)-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-((5-fluoropyrimidin- 2-yl)ethynyl)-1H-indazole-3- carboxamide	***	1.69	609

249		1-(2-((2S,4R)-2-((6-(2- chlorophenyl)pyridin-2- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(pyrimidin-2- ylethynyl)-1H-indazole-3- carboxamide	***	1.91	623

250		1-(2-((2S,4R)-2-((2′-chloro-2-fluoro- [1,1′-biphenyl]-3-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (3-(pyrimidin-2-ylmethyl)ureido)- 1H-indazole-3-carboxamide	***	1.69	688

251		1-(2-((2S,4R)-2-((6-bromopyridin-2- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(3,3- difluoropiperidine-1-carboxamido)- 1H-indazole-3-carboxamide	***	1.59	651

252		1-(2-((2S,4R)-2-((6-bromopyridin-2- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(pyridin-2- ylethynyl)-1H-indazole-3- carboxamide	***	1.60	590

253		1-(2-((1R,3S,5R)-3-((6-bromopyridin- 2-yl)carbamoyl)-2- azabicyclo[3.1.0]hexan-2-yl)-2- oxoethyl)-5-(3,3-difluoropiperidine-1- carboxamido)-1H-indazole-3- carboxamide	***	1.75	645

254		1-(2-((2S,4R)-2-((6-bromopyridin-2- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(4- morpholinophenyl)-1H-indazole-3- carboxamide	***	1.82	650

255		1-(2-((2S,4R)-2-((2′-chloro-2,4′- difluoro-[1,1′-biphenyl]-3- yl)carbamoyl)-4-fluoropyrrolidin-1- yl)-2-oxoethyl)-5-(pyrimidin-2- ylethynyl)-1H-indazole-3- carboxamide		2.04	658

256		1-(2-((2S,4R)-2-((6-(1H-pyrazol-4- yl)pyridin-2-yl)carbamoyl)-4- fluoropyrrolidin-1-yl)-2-oxoethyl)-5- (pyrimidin-2-ylethynyl)-1H-indazole- 3-carboxamide		1.11	579

257		1-(2-((1R,3S,5R)-3-((3-chloro-2- fluorobenzyl)carbamoyl)-6,6- difluoro-2-azabicyclo[3.2.0]heptan- 2-yl)-2-oxoethyl)-5-(pyrimidin-2- ylethynyl)-1H-indazole-3- carboxamide		1.79	622

258		1-(2-((2S,4R)-4-fluoro-2-(2,3,4,9- tetrahydro-1H-pyrido[3,4-b]indole-2- carbonyl)pyrrolidin-1-yl)-2-oxoethyl)- 5-(pyrimidin-2-ylethynyl)-1H- indazole-3-carboxamide	*	1.65

Example 22. Human Factor D Assay

Human factor D (purified from human serum, Complement Technology, Inc.) at 80 nM final concentration is incubated with test compound at various concentrations for 5 minutes at room temperature in 50 mM Tris, 1M NaCl, pH 7.5. A synthetic substrate Z-L-Lys-SBzl and DTNB (Ellman's reagent) are added to final concentrations of 100 M each. The increase in color is recorded at OD₄₀₅nm in a microplate in kinetic mode over 30 minutes with 30 second time points in a spectrofluorimeter. IC₅₀values are calculated by non-linear regression from the percentage of inhibition of complement factor D activity as a function of test compound concentration.

Example 23. Hemolysis Assay

The hemolysis assay was previously described by G. Ruiz-Gomez, et al., J. Med. Chem. (2009) 52: 6042-6052. In our assay red blood cells (RBC), rabbit erythrocyctes (purchased from Complement Technologies), are washed using GVB Buffer (0.1% gelatin, 5 mM Veronal, 145 mM NaCl, 0.025% NaN₃, pH 7.3) plus 10 mM final Mg-EGTA. Cells are used at a concentration of 1×10⁸cells/mL. Prior to the hemolysis assay, the optimum concentration of Normal Human Serum (NHS) needed to achieve 100% lysis of rabbit erythrocytes is determined by titration. NHS (Complement Technologies) is incubated with inhibitor for 15 min at 37° C., rabbit erythrocytes in buffer were added and incubated for an additional 30 min at 37° C. Positive control (100% lysis) consists of serum and RBC and negative control (0% lysis) of Mg-EGTA buffer and RBC only. Samples are centrifuged at 2000 g for 5 min, and supernatants collected. Optical density of the supernatant is monitored at 405 nm using a UV/visible spectrophotometer. Percentage lysis in each sample is calculated relative to positive control (100% lysis).

Claims

What is claimed is:

1. A compound of the formula

or a pharmaceutically acceptable salt thereof, wherein

Q¹is N(R¹) or C(R¹R^1′);

Q²is C(R²R^2′), C(R²R^2′)—C(R²R^2′), or C(R²R^2′)O;

Q³is N(R³), S, or C(R³R^3′)

(a) X¹and X²are independently N or CH, or

(b) X¹and X²together are C═C;

R¹, R^1′, R², R^2′, R³, and R^3′ are independently chosen at each occurrence from (c) and (d)

(c) hydrogen, halogen, hydroxyl, nitro, cyano, amino, C₁-C₆alkyl, C₂-C₆alkenyl, C₁-C₆alkoxy, C₂-C₆alkynyl, C₂-C₆alkanoyl, C₁-C₆thioalkyl, hydroxyC₁-C₆alkyl, aminoC₁-C₆alkyl, —C₀-C₄alkylNR⁹R¹⁰, —C(O)OR⁹, —OC(O)R⁹, —NR⁹C(O)R¹⁰, —C(O)NR⁹R¹⁰, —OC(O)NR⁹R¹⁰,

—NR⁹C(O)OR¹⁰, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy, where R⁹and R¹⁰are independently chosen at each occurrence from hydrogen, C₁-C₆alkyl, and (C₃-C₇cycloalkyl)C₀-C₄alkyl;

(d) —C₀-C₄alkyl(C₃-C₇cycloalkyl) and —O—C₀-C₄alkyl(C₃-C₇cycloalkyl);

Additionally any one of the following rings (e), (f), (g), (h), (i), or (j) may be present:

(e) R¹and R^1′ or R³and R^3′ may be taken together to form a 3- to 6-membered carbocyclic spiro ring or a 3- to 6-membered heterocyclic spiro ring containing 1 or 2 heteroatoms independently chosen from N, O, or S;

(f) R²and R^2′ may be taken together to form a 3- to 6-membered carbocyclic spiro ring,

(g) R²and R^2′ may be taken together to form a 3- to 6-membered heterocyclic spiro ring,

each of which spiro rings (e), (f), and (g) is unsubstituted or substituted with one or more halogen or methyl substituents;

(h) R¹and R²may be taken together to form a 3-membered carbocyclic ring;

(i) R¹and R²may be taken together to form a 4- to 6-membered carbocyclic ring or a 4- to 6-membered heterocyclic ring containing 1 or 2 heteroatoms independently chosen from N, O, and S;

(j) R²and R³, if bound to adjacent carbon atoms, may be taken together to form a 3- to 6-membered carbocyclic ring or a 3- to 6-membered heterocyclic ring; each of which ring (g), (h), and (i) may be unsubstituted or substituted with 1 or more substituents independently chosen from halogen, hydroxyl, cyano, —COOH, C₁-C₄alkyl, C₂-C₄alkenyl, C₁-C₄alkoxy, C₂-C₄alkanoyl, hydroxyC₁-C₄alkyl, (mono- and di-C₁-C₄alkylamino)C₀-C₄alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —O—C₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

A is a heterocyclic group chosen from (k) and (l) where (k) is

and (l) is

X⁴is B(OH) and Y is CHR⁹; or

X⁴is CHR⁹and Y is B(OH);

R⁴is (m) or (n)

(m) —CHO, —CONH₂, or C₂-C₆alkanoyl;

(n) hydrogen, —SO₂NH₂, —C(CH₂)F, —CH(CF₃)NH₂, C₁-C₆alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C(O)C₀-C₂alkyl(C₃-C₇cycloalkyl),

each of which R⁴other than hydrogen, —CHO, and —CONH₂, is unsubstituted or substituted with one or more of amino, imino, halogen, hydroxyl, cyano, cyanoimino, C₁-C₂alkyl, C₁-C₂alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

R⁵and R⁶are independently chosen from (o) and (p)

(o) —CHO, —C(O)NH₂, —C(O)NH(CH₃), or C₂-C₆alkanoyl;

(p) hydrogen, hydroxyl, halogen, cyano, nitro, —COOH, —SO₂NH₂, vinyl, C₁-C₆alkyl, C₂-C₆alkenyl, C₁-C₆alkoxy, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C(O)C₀-C₄alkyl(C₃-C₇cycloalkyl),

—P(O)(OR⁹)₂, —OC(O)R⁹, —C(O)OR⁹, —C(O)N(CH₂CH₂R⁹)(R¹⁰), —NR⁹C(O)R¹⁰, phenyl, or 5- to 6-membered heteroaryl,

each R⁵and R⁶other than hydrogen, hydroxyl, cyano, and —COOH is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, amino, imino, cyano, cyanoimino, C₁-C₂alkyl, C₁-C₄alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

R^6′ is hydrogen, halogen, hydroxyl, C₁-C₄alkyl, or C₁-C₄alkoxy; or

R⁶and R^6′ may be taken together to form an oxo, vinyl, or imino group;

R⁷is hydrogen, C₁-C₆alkyl, or —C₀-C₄alkyl(C₃-C₇cycloalkyl);

R⁸and R^8′ are independently chosen from hydrogen, halogen, hydroxyl, C₁-C₆alkyl, C₁-C₆alkoxy, and (C₁-C₄alkylamino)C₀-C₂alkyl, or R⁸and R^8′ are taken together to form an oxo group;

R¹⁶is 0 or 1 or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

R¹⁹is hydrogen, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, —SO₂C₁-C₆alkyl, (mono- and di-C₁-C₆alkylamino)C₁-C₄alkyl, —C₀-C₄alkyl(C₃-C₇cycloalkyl), each of which R¹⁹other than hydrogen is substituted with 0 or 1 or more substituents independently chosen from halogen, hydroxyl, amino, —COOH, and —C(O)OC₁-C₄alkyl;

X¹¹is N or CR¹¹;

X¹²is N or CR¹²;

X¹³is N or CR¹³;

X¹⁴is N or CR¹⁴;

X¹⁵is N or CR¹⁵;

where no more than 2 of X¹¹, X¹², X¹³, X¹⁴, and X¹⁵are N;

R¹¹, R¹⁴, and R¹⁵are independently chosen at each occurrence from hydrogen, halogen, hydroxyl, nitro, cyano, —O(PO)(OR⁹)₂, —(PO)(OR⁹)₂, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₁-C₆thioalkyl, —C₀-C₄alkyl(mono- and di-C₁-C₆alkylamino), —C₀-C₄alkyl(C₃-C₇cycloalkyl), —C₀-C₄alkoxy(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

R¹²and R¹³are independently chosen from (q), (r), and (s),

(q) hydrogen, halogen, hydroxyl, nitro, cyano, amino, —COOH, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy,

(r) C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, C₂-C₆alkenyloxy, —C(O)OR⁹, C₁-C₆thioalkyl, —C₀-C₄alkylNR⁹R¹⁰, —C(O)NR⁹R¹⁰, —SO₂R⁹R¹⁰, —SO₂NR⁹R¹⁰, —OC(O)R⁹, and —C(NR⁹)NR⁹R¹⁰, each of which (r) is unsubstituted or substituted with one or more substituents independently selected from halogen, hydroxyl, nitro, cyano, amino, —COOH,

—CONH₂C₁-C₂haloalkyl, and C₁-C₂haloalkoxy, and each of which (r) is also optionally substituted with one substituent chosen from phenyl and 4- to 7-membered heterocycle containing 1, 2, or 3 heteroatoms independently chosen from N, O, and S; which phenyl or 4- to 7-membered heterocycle is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl)(C₃-C₇cycloalkyl), C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

(s) —C₂-C₆alkynyl, —C₂-C₆alkynylR²³, C₂-C₆alkanoyl, -JC₃-C₇cycloalkyl, —B(OH)₂, -JC(O)NR⁹R²³,

-JOSO₂OR²¹, —C(O)(CH₂)_1-4S(O)R²¹, —O(CH₂)_1-4S(O)NR²¹NR²², -JOP(O)(OR²¹)(OR²²), -JP(O)(OR²¹)(R²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JSP(O)(OR²¹)(R²²), -JSP(O)(OR²¹)(R²²), -JSP(O)(R²¹)(R²²), -JNR⁹P(O)(NHR²¹)(NHR²²),

-JNR⁹P(O)(OR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(OR²²), -JC(S)R²¹, -JNR²¹SO₂R²², -JNR⁹S(O)NR²¹R²², -JNR⁹SO₂NR¹⁰R²², -JSO₂NR⁹COR²², —O(CH₂)_1-4SO₂NR²¹R²², -JSO₂NR⁹CONR²¹R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², -JOC(O)NR²⁴R²⁵, -JNR⁹C(O)OR¹⁰, -JNR⁹C(O)OR²³, -JNR²¹OC(O)R²², —(CH₂)_1-4C(O)NR²¹R²², -JC(O)NR²⁴R²⁵, -JNR⁹C(O)R²¹, -JC(O)R²¹,

-JNR⁹C(O)NR⁹R¹⁰, -JNR⁹C(O)NR¹⁰R²³, -JNR⁹C(O)NR²⁴R²⁵, —CCR²¹, —(CH₂)₁—₄OC(O)R²¹,

-JC(O)OR²³, —C₂-C₄alkylR²³, —C₂-C₄alkenylR²³, —C₂-C₄alkynylR²³, and -Jparacyclophane;

where J is independently chosen at each occurrence from a covalent bond, C₁-C₄alkylene, —OC₁-C₄alkylene, C₂-C₄alkenylene, and C₂-C₄alkynylene;

R²¹and R²²are independently chosen at each occurrence from hydrogen, hydroxyl, cyano, amino, C₁-C₆alkyl, C₁-C₆alkyl, C₁-C₆alkoxy, (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, —C₁-C₄alkylOC(O)OC₁-C₆alkyl, —C₁-C₄alkylOC(O)C₁-C₆alkyl, —C₁-C₄alkylC(O)OC₁-C₆alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycle)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S;

R²³is independently chosen at each occurrence from (C₃-C₇cycloalkyl)C₀-C₄alkyl, (phenyl)C₀-C₄alkyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and (5- or 6-membered unsaturated or aromatic heterocycle)C₀-C₄alkyl having 1, 2, or 3 heteroatoms independently chosen from N, O, and S;

R²⁴and R²⁵are taken together with the nitrogen to which they are attached to form a 4- to 7-membered monocyclic heterocycloalkyl group, or a 6- to 10-membered bicyclic heterocyclic group having fused, spiro, or bridged rings;

each of which (s) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH,

—CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy; and

L is either (t), (u), or (v) where

(t) is a group of the formula

where R¹⁷is hydrogen or C₁-C₆alkyl and R¹⁸and R^18′ are independently chosen from hydrogen, halogen, and methyl; and m is 0, 1, 2, or 3; and

(u) is a bond,

(v) or a group of the formula

B is a monocyclic or bicyclic carbocyclic or carbocyclic-oxy group or a monocyclic, bicyclic, or tricyclic heterocyclic group having 1, 2, 3, or 4 heteroatoms independently selected from N, O, and S and from 4 to 7 ring atoms per ring, or

B is a C₂-C₆alkenyl or C₂-C₆alkynyl group;

each of which B is unsubstituted or substituted with one or more substituents independently chosen from (w) and (x) and 0 or 1 substituents chosen from (y) and (z)

(w) halogen, hydroxyl, —COOH, cyano, C₁-C₆alkyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, —C₀-C₄alkylNR⁹R¹⁰, —SO₂R⁹, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

(x) nitro, C₂-C₆alkenyl, C₂-C₆alkynyl, C₁-C₆thioalkyl, -JC₃-C₇cycloalkyl, —B(OH)₂, -JC(O)NR⁹R²³,

-JOSO₂OR²¹, —C(O)(CH₂)_1-4S(O)R²¹, —O(CH₂)_1-4S(O)NR²¹R²², -JOP(O)(OR²¹)(OR²²), -JP(O)(OR²¹)(R²²), -JOP(O)(OR²¹)R²², -JP(O)(OR²¹)R²², -JOP(O)R²¹R²², -JP(O)R²¹R²², -JSP(O)(R²¹)(R²²), -JSP(O)(OR²¹)(R²²), -JSP(O)(R²¹)(R²²), -JNR⁹P(O)(NHR²¹)(NHR²²),

-JNR⁹P(o)(OR²¹)(NHR²²), -JNR⁹P(O)(OR²¹)(OR²²), -JC(S)R²¹, -JNR²¹SO₂R²², -JNR⁹S(O)_nNR¹⁰R²², -JSO₂NR⁹COR²², -JSO₂NR⁹CONR²¹R²², -JNR²¹SO₂R²², -JC(O)NR²¹SO₂R²², -JC(NH₂)NR²², -JC(NH₂)NS(O)₂R²², -JOC(O)NR²¹R²², JNR²¹C(O)OR²²,

-JNR²¹OC(O)R²², —(CH₂)_1-4C(O)NR²¹R²², -JC(O)R²⁴R²⁵, -JNR⁹C(O)R²¹, -JC(O)R²¹, -JNR⁹C(O)NR¹⁰R²², —CCR²¹, —(CH₂)_1-4OC(O)R²¹, and -JC(O)OR²³;

each of which (x) may be unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, amino, oxo, —B(OH)₂, —Si(CH₃)₃, —COOH,

—CONH₂, —P(O)(OH)₂, C₁-C₆alkyl, C₁-C₆alkoxy, —C₀-C₂alkyl(mono- and di-C₁-C₄alkylamino), C₁-C₆alkylester, C₁-C₄alkylamino, C₁-C₄hydroxylalkyl, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

(y) naphthyl, naphthyloxy, indanyl, (4- to 7-membered heterocycloalkyl)C₀-C₄alkyl containing 1 or 2 heteroatoms chosen from N, O, and S, and bicyclic heterocycle containing 1, 2, or 3 heteroatoms independently chosen from N, O, and S, and containing 4- to 7-ring atoms in each ring; each of which (y) is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl(C₃-C₇cycloalkyl), —SO₂R⁹, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy; and

(z) tetrazolyl, (phenyl)C₀-C₂alkyl, (phenyl)C₁-C₂alkoxy, phenoxy, and 5- or 6-membered heteroaryl containing 1, 2, or 3 heteroatoms independently chosen from N, O, B, and S, each of which (z) is unsubstituted or substituted with one or more substituents independently chosen from halogen, hydroxyl, nitro, cyano, C₁-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkanoyl, C₁-C₆alkoxy, (mono- and di-C₁-C₆alkylamino)C₀-C₄alkyl, C₁-C₆alkylester, —C₀-C₄alkyl(C₃-C₇cycloalkyl),

—SO₂R⁹, —OSi(CH₃)₂C(CH₃)₃, C₁-C₂haloalkyl, and C₁-C₂haloalkoxy;

wherein X²is nitrogen or at least one of (d), (e), (g), (i), (l), (n), (p), (s), (v), (x), and (y) is present.

2. The compound of claim 1, wherein the formula comprises

3. The compound of claim 1, wherein the formula comprises

4. The compound of claim 1, wherein the formula comprises

5. The compound of claim 1, wherein the formula comprises:

6. The compound of claim 1, wherein the formula comprises:

7. The compound of claim 1, wherein the formula comprises:

8. The compound of claim 1, wherein the formula comprises:

9. The compound of claim 1, wherein the formula comprises:

10. The compound of claim 1, wherein the formula comprises:

11. The compound of claim 1, wherein the formula comprises:

12. The compound of claim 1, wherein the formula comprises:

13. The compound of claim 1, wherein the formula comprises:

14. The compound of claim 1, wherein the formula comprises:

15. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

16. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

17. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

18. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

19. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

20. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

21. The compound of claim 1, wherein the formula comprises:

where m is 0 or 1.

22. A pharmaceutical composition comprising a compound of claim 1, together with a pharmaceutically acceptable carrier.

23. A method of treating or preventing a complement D-related disorder comprising administering a therapeutically effective amount of a compound of claim 1, to a patient in need of such treatment.

24. The method of claim 23, wherein the disorder is paroxysmal nocturnal hemoglobinuria.

25. The method of claim 23, wherein the disorder is age-related macular degeneration.

26. The method of claim 23, wherein the disorder is selected from inflammation of the eye, retinal degeneration, myasthenia gravis, C3 glomerulonephritis, abdominal aortic aneurysm, or atypical hemolytic uremic syndrome.