US20170107508A1 - Methods for purifying nucleic acids and devices thereof - Google Patents

Methods for purifying nucleic acids and devices thereof Download PDF

Info

Publication number
US20170107508A1
US20170107508A1 US15/335,079 US201615335079A US2017107508A1 US 20170107508 A1 US20170107508 A1 US 20170107508A1 US 201615335079 A US201615335079 A US 201615335079A US 2017107508 A1 US2017107508 A1 US 2017107508A1
Authority
US
United States
Prior art keywords
adapter
well plate
electromagnetic feature
electrical signal
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US15/335,079
Inventor
William Ross Higgins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
True Health Diagnostics LLC
Original Assignee
True Health Diagnostics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by True Health Diagnostics LLC filed Critical True Health Diagnostics LLC
Priority to US15/335,079 priority Critical patent/US20170107508A1/en
Publication of US20170107508A1 publication Critical patent/US20170107508A1/en
Priority to US15/943,150 priority patent/US20190078079A1/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/08—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
    • B01J19/12—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor employing electromagnetic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/08—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
    • B01J2219/12—Processes employing electromagnetic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Definitions

  • This technology generally relates to nucleic acid purification and, more particularly, to improved methods and devices for extracting nucleic acids from samples with increased throughput.
  • Magnetic stands of varying shapes, sizes and strengths are commercially available from many vendors for use in magnetic particle based nucleic acid extractions.
  • the nucleic acids remains in the same plate for all wash steps and are eluted from the magnetic particles at the last step.
  • the plate must be moved to and from the magnetic stand depending on the step of the extraction and/or to and from the shaker for shaking/vortexing steps.
  • nucleic acid extraction uses an electromagnet.
  • the electromagnet can be used to move the magnetic particles (including nucleic acids bound thereto) from one wash plate to the next. Particles are released from the electromagnet into each new plate.
  • the movement of plates between a magnetic stand and a shaker, and/or of magnetic particles between different plates, is a rate limiting step in the extraction process which reduces throughput.
  • a nucleic acid purification device which includes a housing and an adapter operatively attached to the housing.
  • the adapter is configured to receive at least one well plate which includes a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles.
  • the device also includes a motor disposed within the housing and configured to selectively move at least the adapter to thereby disrupt or shake the contents of the plurality of wells when the at least one well plate is received by the adapter.
  • the device further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the adapter.
  • an adapter apparatus for facilitating nucleic acid purification.
  • the adapter is configured to mount to a well plate shaker device and includes a first attachment device for receiving at least one well plate, the at least one well plate including a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles.
  • the adapter further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the first attachment device.
  • a method for purifying nucleic acids includes introducing a sample including a plurality of nucleic acids and a plurality of paramagnetic particles to one or more of a plurality of wells of at least one well plate, wherein the at least one well plate is attached to an adapter coupled to a well plate shaker device.
  • An electrical signal is provided to at least one electromagnetic feature of the adapter to thereby magnetize at least a portion of the plurality of paramagnetic particles and bind the plurality of paramagnetic particles to the plurality of nucleic acids.
  • One or more buffers are introduced to the one or more of the plurality of wells of the at least one well plate. At least a portion of the plurality of nucleic acids is extracted to produce a purified nucleic acid composition.
  • nucleic acids can be mixed, whether unbound or bound to magnetic particles, on a shaker unit without moving well plates with the nucleic acids, such as to a magnetic stand for example. Accordingly, nucleic acid purification processes can advantageously proceed with higher throughput, decreased extraction time, less user intervention, and reduced instrument wear caused by increased plate movement between devices.
  • FIG. 1 is a perspective view of an exemplary adapter apparatus with an electromagnetic feature including a plurality of vertical projections
  • FIG. 2 is a perspective view of another exemplary adapter apparatus with an electromagnetic feature including a plurality of rails;
  • FIG. 3 is an exemplary method for purifying nucleic acids.
  • exemplary adapters 100 and 200 for facilitating nucleic acid purification are illustrated.
  • the adapters 100 and 200 are each configured to receive at least one well plate at a first attachment device, optionally at a top portion.
  • the well plate includes a plurality of wells for receiving contents including at least a sample, including nucleic acids to be extracted, and a plurality of paramagnetic particles.
  • the adapters 100 and 200 are also configured to attach to a housing of a well plate shaker device of a liquid handling system, for example, or any other device including a motor disposed within a housing and configured to selectively move at least one of the adapters 100 and 200 to thereby disrupt or shake the contents of the wells.
  • the adapters 100 and 200 in this example include at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the paramagnetic particles are received by the wells and the well plate is received by the first attachment device, as described and illustrated in more detail later.
  • the electromagnetic feature can include a ferromagnetic material at least partially surrounded by an electrically conductive material, although other types of materials can also be used.
  • the adapters 100 and 200 include a diamagnetic material, although other types of materials can also be used.
  • the adapters 100 and 200 optionally include a top portion and the electromagnetic feature can be disposed proximate the top portion and between the top portion and the well plate and/or the electromagnet feature can be integrated with the adapter as a single unit, for example.
  • the electromagnetic feature can include a substantially planar top surface configured to be disposed adjacent to a bottom portion of the well plate.
  • the electromagnetic feature is configured to produce a magnetic field in a direction substantially perpendicular to the bottom portion of the well plate and a top surface of at least one of the adapters 100 and 200 when the electromagnetic feature receives an electrical signal.
  • the adapter 100 includes an electromagnet feature including a plurality of protrusions 102 extending away from a top portion of the adapter 100 and between at least a portion of the plurality of wells.
  • the at least one electromagnetic feature includes a plurality of depressions and is positioned between at least a portion of the wells.
  • the adapter 200 includes an electromagnetic feature including a plurality of rails 202 extending away from a top portion of the adapter 200 .
  • the electromagnet feature optionally includes an electrical interface coupled to a switch of the well plate shaker device to which the adapter is configured to attach.
  • the switch is electrically connected to an electrical signal source and configured to operatively supply the electrical signal to the electromagnetic feature when engaged by a user or a controller programmed by the user to supply the electrical signal to the electromagnetic feature at one or more selected times.
  • a well plate is securely attached to an adapter, such as adapter 100 for purposes of this example only although any other type of adapter with an electromagnetic feature can also be used. Additionally, in step 300 , the adapter 100 is coupled to a well plate shaker device and connected to an electrical interface.
  • an adapter such as adapter 100 for purposes of this example only although any other type of adapter with an electromagnetic feature can also be used.
  • the adapter 100 is coupled to a well plate shaker device and connected to an electrical interface.
  • a sample with nucleic acids such as a human sample (e.g., blood) is introduced to one or more wells of the well plate. Additionally, paramagnetic particles are introduced to the one or more wells of the well plate, as is known in the art and will not be described in detail herein.
  • step 304 the adapter 100 is shaken by the well plate shaker device to thereby mix the sample and the plurality of paramagnetic particles while the plurality of nucleic acids are unbound from the plurality of paramagnetic particles to thereby increase the effectiveness of the particle binding.
  • step 306 an electrical signal is provided to the electromagnetic feature of the adapter 100 to thereby magnetize at least a portion of the paramagnetic particles and bind the paramagnetic particles to the nucleic acids in the sample.
  • step 308 at least a portion of the nucleic acids in the sample are extracted to produce a purified nucleic acid composition.
  • the nucleic acids can be extracted by introducing one or more buffers, such as at least one wash and/or elution buffer for example, to the one or more well plates and subsequently switching off the electrical signal previously supplied to the electromagnetic feature to thereby unbind the paramagnetic particles from the nucleic acids in the sample.
  • nucleic acids can be extracted from a sample without removing the well plate from the adapter or the well plate shaker device. Accordingly, the rate limiting step is not required and an increased number of samples can advantageously be processed in a reduced time period.

Abstract

A nucleic acid purification device includes a housing and an adapter operatively attached to the housing. The adapter is configured to receive at least one well plate which includes a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles. The device also includes a motor disposed within the housing and configured to selectively move at least the adapter to thereby disrupt or shake the contents of the plurality of wells when the at least one well plate is received by the adapter. The device further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the adapter.

Description

  • This application claims the benefit of U.S. Provisional Patent Application No. 61/863,678, filed on Aug. 8, 2013 and U.S. Provisional Patent Application No. 61/823,740, filed on May 15, 2013, each of which is hereby incorporated by reference in its entirety.
    • FIELD
  • This technology generally relates to nucleic acid purification and, more particularly, to improved methods and devices for extracting nucleic acids from samples with increased throughput.
    • BACKGROUND
  • Magnetic stands of varying shapes, sizes and strengths are commercially available from many vendors for use in magnetic particle based nucleic acid extractions. In extraction using magnetic stands, the nucleic acids remains in the same plate for all wash steps and are eluted from the magnetic particles at the last step. However, the plate must be moved to and from the magnetic stand depending on the step of the extraction and/or to and from the shaker for shaking/vortexing steps.
  • Alternative methods for nucleic acid extraction use an electromagnet. For example, the electromagnet can be used to move the magnetic particles (including nucleic acids bound thereto) from one wash plate to the next. Particles are released from the electromagnet into each new plate. The movement of plates between a magnetic stand and a shaker, and/or of magnetic particles between different plates, is a rate limiting step in the extraction process which reduces throughput.
    • SUMMARY
  • In an aspect, a nucleic acid purification device is disclosed which includes a housing and an adapter operatively attached to the housing. The adapter is configured to receive at least one well plate which includes a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles. The device also includes a motor disposed within the housing and configured to selectively move at least the adapter to thereby disrupt or shake the contents of the plurality of wells when the at least one well plate is received by the adapter. The device further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the adapter.
  • In another aspect an adapter apparatus for facilitating nucleic acid purification is disclosed. The adapter is configured to mount to a well plate shaker device and includes a first attachment device for receiving at least one well plate, the at least one well plate including a plurality of wells for receiving contents including at least a sample and a plurality of paramagnetic particles. The adapter further includes at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the first attachment device.
  • In yet another aspect, a method for purifying nucleic acids is disclosed. The method includes introducing a sample including a plurality of nucleic acids and a plurality of paramagnetic particles to one or more of a plurality of wells of at least one well plate, wherein the at least one well plate is attached to an adapter coupled to a well plate shaker device. An electrical signal is provided to at least one electromagnetic feature of the adapter to thereby magnetize at least a portion of the plurality of paramagnetic particles and bind the plurality of paramagnetic particles to the plurality of nucleic acids. One or more buffers are introduced to the one or more of the plurality of wells of the at least one well plate. At least a portion of the plurality of nucleic acids is extracted to produce a purified nucleic acid composition.
  • With this technology, nucleic acids can be mixed, whether unbound or bound to magnetic particles, on a shaker unit without moving well plates with the nucleic acids, such as to a magnetic stand for example. Accordingly, nucleic acid purification processes can advantageously proceed with higher throughput, decreased extraction time, less user intervention, and reduced instrument wear caused by increased plate movement between devices.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 is a perspective view of an exemplary adapter apparatus with an electromagnetic feature including a plurality of vertical projections;
  • FIG. 2 is a perspective view of another exemplary adapter apparatus with an electromagnetic feature including a plurality of rails; and
  • FIG. 3 is an exemplary method for purifying nucleic acids.
    •  DETAILED DESCRIPTION
  • Referring to FIGS. 1 and 2, exemplary adapters 100 and 200 for facilitating nucleic acid purification are illustrated. In this example, the adapters 100 and 200 are each configured to receive at least one well plate at a first attachment device, optionally at a top portion. The well plate includes a plurality of wells for receiving contents including at least a sample, including nucleic acids to be extracted, and a plurality of paramagnetic particles. The adapters 100 and 200 are also configured to attach to a housing of a well plate shaker device of a liquid handling system, for example, or any other device including a motor disposed within a housing and configured to selectively move at least one of the adapters 100 and 200 to thereby disrupt or shake the contents of the wells.
  • The adapters 100 and 200 in this example include at least one electromagnetic feature configured to selectively receive an electrical signal and thereby magnetize at least a portion of the plurality of paramagnetic particles when the paramagnetic particles are received by the wells and the well plate is received by the first attachment device, as described and illustrated in more detail later. The electromagnetic feature can include a ferromagnetic material at least partially surrounded by an electrically conductive material, although other types of materials can also be used.
  • Optionally, the adapters 100 and 200 include a diamagnetic material, although other types of materials can also be used. In some examples, the adapters 100 and 200 optionally include a top portion and the electromagnetic feature can be disposed proximate the top portion and between the top portion and the well plate and/or the electromagnet feature can be integrated with the adapter as a single unit, for example.
  • For example, the electromagnetic feature can include a substantially planar top surface configured to be disposed adjacent to a bottom portion of the well plate. In these examples, the electromagnetic feature is configured to produce a magnetic field in a direction substantially perpendicular to the bottom portion of the well plate and a top surface of at least one of the adapters 100 and 200 when the electromagnetic feature receives an electrical signal.
  • Referring more specifically to FIG. 1, in one example, the adapter 100 includes an electromagnet feature including a plurality of protrusions 102 extending away from a top portion of the adapter 100 and between at least a portion of the plurality of wells. In another example, the at least one electromagnetic feature includes a plurality of depressions and is positioned between at least a portion of the wells. Referring more specifically to FIG. 2, in yet another example, the adapter 200 includes an electromagnetic feature including a plurality of rails 202 extending away from a top portion of the adapter 200.
  • In order to receive the electrical signal, the electromagnet feature optionally includes an electrical interface coupled to a switch of the well plate shaker device to which the adapter is configured to attach. The switch is electrically connected to an electrical signal source and configured to operatively supply the electrical signal to the electromagnetic feature when engaged by a user or a controller programmed by the user to supply the electrical signal to the electromagnetic feature at one or more selected times.
  • Referring to FIG. 3, an exemplary method for purifying nucleic acids will now be described. In step 300 in this example, a well plate is securely attached to an adapter, such as adapter 100 for purposes of this example only although any other type of adapter with an electromagnetic feature can also be used. Additionally, in step 300, the adapter 100 is coupled to a well plate shaker device and connected to an electrical interface.
  • In step 302, a sample with nucleic acids, such as a human sample (e.g., blood) is introduced to one or more wells of the well plate. Additionally, paramagnetic particles are introduced to the one or more wells of the well plate, as is known in the art and will not be described in detail herein.
  • In step 304, the adapter 100 is shaken by the well plate shaker device to thereby mix the sample and the plurality of paramagnetic particles while the plurality of nucleic acids are unbound from the plurality of paramagnetic particles to thereby increase the effectiveness of the particle binding.
  • In step 306, an electrical signal is provided to the electromagnetic feature of the adapter 100 to thereby magnetize at least a portion of the paramagnetic particles and bind the paramagnetic particles to the nucleic acids in the sample.
  • In step 308, at least a portion of the nucleic acids in the sample are extracted to produce a purified nucleic acid composition. The nucleic acids can be extracted by introducing one or more buffers, such as at least one wash and/or elution buffer for example, to the one or more well plates and subsequently switching off the electrical signal previously supplied to the electromagnetic feature to thereby unbind the paramagnetic particles from the nucleic acids in the sample.
  • By this technology, nucleic acids can be extracted from a sample without removing the well plate from the adapter or the well plate shaker device. Accordingly, the rate limiting step is not required and an increased number of samples can advantageously be processed in a reduced time period.
  • Having thus described the basic concept of the invention, it will be rather apparent to those skilled in the art that the foregoing detailed disclosure is intended to be presented by way of example only, and is not limiting. Various alterations, improvements, and modifications will occur and are intended to those skilled in the art, though not expressly stated herein. These alterations, improvements, and modifications are intended to be suggested hereby, and are within the spirit and scope of the invention. Additionally, the recited order of processing elements or sequences, or the use of numbers, letters, or other designations therefore, is not intended to limit the claimed processes to any order except as may be specified in the claims. Accordingly, the invention is limited only by the following claims and equivalents thereto.

Claims (15)

1. A nucleic acid purification device, comprising:
a housing and an adapter operatively attached to the housing, the adapter having at least one electromagnetic feature and configured to receive at least one well plate, the at least one well plate comprising a plurality of wells for receiving contents;
a motor disposed within the housing and configured to selectively move at least the adapter to thereby disrupt or shake the contents of the plurality of wells when the at least one well plate is received by the adapter; and
wherein the at least one electromagnetic feature is configured to selectively receive an electrical signal and thereby magnetize at least a portion of a plurality of paramagnetic particles when the plurality of paramagnetic particles are received by the plurality of wells and the at least one well plate is received by the adapter.
2. The device of claim 1 wherein the adapter is further configured to receive the at least one well plate securely and at a top portion of the adapter.
3. The device of claim 1 wherein the at least one electromagnetic feature is disposed between a top portion of the adapter and the at least one well plate or the at least one electromagnetic feature is integrated with the adapter.
4. The device of claim 1 wherein the at least one electromagnetic feature comprises a plurality of protrusions extending away from a top portion of the adapter or a plurality of depressions and is positioned between at least a portion of the plurality of wells.
5. The device of claim 1 wherein the at least one electromagnetic feature comprises a substantially planar top surface configured to be disposed adjacent to a bottom portion of the at least one well plate.
6. The device of claim 5 wherein the at least one electromagnetic feature is configured to produce a magnetic field in a direction substantially perpendicular to the bottom portion of the at least one well plate when the electromagnetic feature receives the electrical signal.
7. The device of claim 1 further comprising a switch electrically connected to an electrical signal source, the switch configured to selectively supply the electrical signal to the at least one electromagnetic feature when engaged by a user or a controller programmed by the user to supply the electrical signal to the at least one electromagnetic feature at one or more selected times.
8. The device of claim 1 wherein the electromagnetic feature comprises a ferromagnetic material at least partially surrounded by an electrically conductive material.
9. The device of claim 1 wherein the at least one electromagnetic feature is configured to be disposed between the adapter and the at least one well plate, the adapter comprises at least one diamagnetic material, and the at least one electromagnetic feature is configured to produce a magnetic field in a direction substantially perpendicular to a top surface of the adapter when the electromagnetic feature receives the electrical signal.
10-15. (canceled)
16. A method for purifying nucleic acids, the method comprising:
introducing a plurality of nucleic acids and a plurality of paramagnetic particles to one or more of a plurality of wells of at least one well plate, wherein the at least one well plate is attached to an adapter coupled to a well plate shaker device;
allowing at least a portion of the nucleic acids to bind to at least a portion of the paramagnetic particles to provide nucleic acid-paramagnetic particle complexes;
providing an electrical signal to at least one electromagnetic feature of the adapter to thereby magnetize at least a portion of the plurality of the nucleic acid-paramagnetic particle complexes; and
extracting at least a portion of the plurality of nucleic acids to produce a purified nucleic acid composition;
wherein the steps are performed without removing the well plate from the adaptor, the well plate shaker device, or both.
17. The method of claim 16 further comprising shaking the adapter with the well plate shaker device to thereby mix the plurality of nucleic acids and the plurality of paramagnetic particles when the plurality of nucleic acids are unbound from and bound to the plurality of paramagnetic particles.
18. The method of claim 17 wherein the steps are repeated for a plurality of well plates to thereby provide increased throughput.
19. The method of claim 16 further comprising introducing at least one wash buffer or at least one elution buffer subsequent to providing the electrical signal to the at least one electromagnetic feature.
20. The method of claim 16 further comprising, prior to providing the electrical signal to the at least one electromagnetic feature, the steps of:
coupling the adapter to the well plate shaker device at an electrical interface; and
securely attaching the at least one well plate to the adapter.
US15/335,079 2013-05-15 2016-10-26 Methods for purifying nucleic acids and devices thereof Abandoned US20170107508A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US15/335,079 US20170107508A1 (en) 2013-05-15 2016-10-26 Methods for purifying nucleic acids and devices thereof
US15/943,150 US20190078079A1 (en) 2013-05-15 2018-04-02 Methods for purifying nucleic acids and devices thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361823740P 2013-05-15 2013-05-15
US201361863678P 2013-08-08 2013-08-08
US14/278,910 US9504981B2 (en) 2013-05-15 2014-05-15 Methods for purifying nucleic acids and devices thereof
US15/335,079 US20170107508A1 (en) 2013-05-15 2016-10-26 Methods for purifying nucleic acids and devices thereof

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US14/278,910 Continuation US9504981B2 (en) 2013-05-15 2014-05-15 Methods for purifying nucleic acids and devices thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US15/943,150 Continuation US20190078079A1 (en) 2013-05-15 2018-04-02 Methods for purifying nucleic acids and devices thereof

Publications (1)

Publication Number Publication Date
US20170107508A1 true US20170107508A1 (en) 2017-04-20

Family

ID=51896277

Family Applications (3)

Application Number Title Priority Date Filing Date
US14/278,910 Expired - Fee Related US9504981B2 (en) 2013-05-15 2014-05-15 Methods for purifying nucleic acids and devices thereof
US15/335,079 Abandoned US20170107508A1 (en) 2013-05-15 2016-10-26 Methods for purifying nucleic acids and devices thereof
US15/943,150 Abandoned US20190078079A1 (en) 2013-05-15 2018-04-02 Methods for purifying nucleic acids and devices thereof

Family Applications Before (1)

Application Number Title Priority Date Filing Date
US14/278,910 Expired - Fee Related US9504981B2 (en) 2013-05-15 2014-05-15 Methods for purifying nucleic acids and devices thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
US15/943,150 Abandoned US20190078079A1 (en) 2013-05-15 2018-04-02 Methods for purifying nucleic acids and devices thereof

Country Status (1)

Country Link
US (3) US9504981B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10961491B1 (en) * 2016-03-08 2021-03-30 Sansure Biotech Inc. Magnetic bead-based nucleic acid extraction apparatus and use method

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD854185S1 (en) * 2017-02-03 2019-07-16 Biotix, Inc. Pipette tip receptacle plate
US11173492B2 (en) 2017-02-03 2021-11-16 Biotix, Inc. Efficiently nested pipette tip arrays and related methods
CN107287114A (en) * 2017-07-01 2017-10-24 中山大学 The magnetic separating device and method of a kind of biological specimen

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010055545A1 (en) * 2000-06-22 2001-12-27 Shimadzu Corporation Drainage system
US20040022667A1 (en) * 2001-11-07 2004-02-05 Sunggyu Lee Microbial decontamination and detoxification system and method
US20090181359A1 (en) * 2007-10-25 2009-07-16 Lou Sheng C Method of performing ultra-sensitive immunoassays
US20100285573A1 (en) * 2006-11-24 2010-11-11 Kwong Joo Leck Apparatus for processing a sample in a liquid droplet and method of using the same
US20110088491A1 (en) * 2009-10-16 2011-04-21 Steve Krueger Heating, shaking, and magnetizing apparatus and method of operating the same

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52143551A (en) * 1976-05-25 1977-11-30 Fuji Zoki Seiyaku Method of diluting and stirring liquid and apparatus therefor
US5897783A (en) * 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
ES2170083T3 (en) * 1993-09-17 2002-08-01 Hoffmann La Roche ANALYZER WITH A DEVICE FOR SEPARATION OF MAGNETIC MICROPARTICLES.
EP1671703A3 (en) * 1998-03-19 2006-07-05 Precision System Science Co., Ltd. Method for making substances in carriers
US6485690B1 (en) * 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
US6508582B2 (en) * 1999-12-23 2003-01-21 Union Scientific Corporation Electromagnetic vibratory microplate shaker
US6659637B2 (en) * 2000-10-03 2003-12-09 Union Scientific Corporation Vertical electromagnetic shaker for biological and chemical specimens
CA2764307C (en) * 2001-06-29 2015-03-03 Meso Scale Technologies, Llc. Assay plates, reader systems and methods for luminescence test measurements
EP1425577A4 (en) * 2001-08-10 2004-12-29 Symyx Technologies Inc Apparatuses and methods for creating and testing pre-formulations and systems for same
DE10156790A1 (en) * 2001-11-19 2003-06-18 Chemagen Biopolymer Technologi Device and method for treating magnetic particles
US7429472B2 (en) * 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US7981362B2 (en) * 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
US7425436B2 (en) * 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
DE202006001514U1 (en) * 2006-02-01 2006-04-06 Berthold Technologies Gmbh & Co. Kg mixing device
EP2000808B1 (en) * 2006-03-28 2022-05-04 Universal Bio Research Co., Ltd. Micro plate treating device and micro plate treating method
EP2394175B1 (en) * 2009-02-09 2016-02-03 caprotec bioanalytics GmbH Devices, systems and methods for separating magnetic particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010055545A1 (en) * 2000-06-22 2001-12-27 Shimadzu Corporation Drainage system
US20040022667A1 (en) * 2001-11-07 2004-02-05 Sunggyu Lee Microbial decontamination and detoxification system and method
US20100285573A1 (en) * 2006-11-24 2010-11-11 Kwong Joo Leck Apparatus for processing a sample in a liquid droplet and method of using the same
US20090181359A1 (en) * 2007-10-25 2009-07-16 Lou Sheng C Method of performing ultra-sensitive immunoassays
US20110088491A1 (en) * 2009-10-16 2011-04-21 Steve Krueger Heating, shaking, and magnetizing apparatus and method of operating the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10961491B1 (en) * 2016-03-08 2021-03-30 Sansure Biotech Inc. Magnetic bead-based nucleic acid extraction apparatus and use method

Also Published As

Publication number Publication date
US20140343268A1 (en) 2014-11-20
US9504981B2 (en) 2016-11-29
US20190078079A1 (en) 2019-03-14

Similar Documents

Publication Publication Date Title
US20190078079A1 (en) Methods for purifying nucleic acids and devices thereof
JP4254924B2 (en) Device and method for processing magnetic particles
US8685322B2 (en) Apparatus and method for the purification of biomolecules
JP4856831B2 (en) Apparatus and method for mixing magnetic particles with a fluid
JP2005511264A5 (en)
AU2006210041B2 (en) Device and method for the elimination of magnetic or magnetizable particles from a liquid
US9694368B2 (en) Magnetic reagent, magnetic reagent kit, method for treating magnetic carriers, and treatment device therefor
JP2013172736A5 (en)
JP4762139B2 (en) Apparatus and method for separating magnetic or magnetizable particles from a liquid
ATE419919T1 (en) SYSTEM AND METHOD FOR TREATING MAGNETIC PARTICLES IN TEST LIQUIDS FOR COLLECTING DNA AND RNA
EP0842704A1 (en) Magnet separator with magnetic compensated release mechanism for separating biological material
US20110205835A1 (en) Method for suspending or re-suspending particles in a solution and apparatus adapted thereto
US20080083291A1 (en) Apparatus and method for separating magnetic particles from a solution
US20200063118A1 (en) Discontinuous Wall Hollow Core Magnet
EP2068145A3 (en) Manipulation of magnetic microparticles in a high pressure liquid system and extraction process
US5628407A (en) Method and apparatus for separation of magnetically responsive spheres
WO2012046131A1 (en) Magnetic tissue homogenizing
CN208917201U (en) Nucleic acid purification system and nucleic acid purification Special magnetic frame for automatic operation
EP3655163A1 (en) Magnetic assisted separation apparatuses and related methods
KR20160148201A (en) Automatic quantitative dispensing device, continuous type gene extraction-amplification apparatus including the device and operating method of the apparatus
JP2010539502A (en) Apparatus and method for treating liquid using magnetic particles
US20200030816A1 (en) Device for magnetic bead separation and resuspension
US11173498B2 (en) Apparatus and method for the analysis; isolation and/or enrichment of target structures in a fluid sample
JP2020535393A (en) Devices and methods for immobilizing biomolecules using magnetic particles
CN115362372A (en) Bead manipulation in consumable materials

Legal Events

Date Code Title Description
STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION