US20160031975A1 - Compositions and Methods for Antibodies Targeting Complement Protein C5 - Google Patents

Compositions and Methods for Antibodies Targeting Complement Protein C5 Download PDF

Info

Publication number
US20160031975A1
US20160031975A1 US14/698,039 US201514698039A US2016031975A1 US 20160031975 A1 US20160031975 A1 US 20160031975A1 US 201514698039 A US201514698039 A US 201514698039A US 2016031975 A1 US2016031975 A1 US 2016031975A1
Authority
US
United States
Prior art keywords
seq
antibody
encoding
antibodies
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US14/698,039
Inventor
Beate Diefenbach-Streiber
Adina Eberth
Braydon Charles Guild
Yong-In Kim
Michael Roguska
Igor Splawski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Priority to US14/698,039 priority Critical patent/US20160031975A1/en
Assigned to NOVARTIS AG reassignment NOVARTIS AG ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: GUILD, BRAYDON CHARLES, KIM, YONG-IN, EBERTH, ADINA, ROGUSKA, MICHAEL, SPLAWSKI, IGOR, DIEFENBACH-STREIBER, BEATE
Publication of US20160031975A1 publication Critical patent/US20160031975A1/en
Priority to US15/354,978 priority patent/US10407496B2/en
Abandoned legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Definitions

  • the present invention relates to antibodies targeting complement protein C5 and compositions and methods of use thereof.
  • complement which is part of the innate immune system, is in host defense. Complement defends against bacterial infection, links adaptive and innate immunity, and disposes immune complexes and the products of inflammatory injury.
  • the defensive functions are accomplished by biologically active products generated in the course of complement activation, which opsonise infectious agents, promote inflammation or lyse susceptible targets (Marzari et al., Eur J Immunol 32:2773-2782 (2002)).
  • the complement system consists of about 25-30 plasma proteins which play a role in the immune system.
  • the complement cascade is activated by at least three major pathways. The classical pathway is typically activated by immune-complexes, the alternative pathway can be activated by unprotected cell surfaces, and the mannose binding lectin (MBL) pathway is initiated by binding of MBL to cell surface carbohydrates (Trendelenburg, Swiss Med Wkly 137:413-417 (2007)).
  • MBL mannose binding lectin
  • C5 is expressed intracellularly as a single pro-05 peptide of 1676 amino acids that consist of an 18 residue signal sequence and an Arg-rich linker sequence (RPRR) situated between the mature N-terminal ⁇ chain and the C-terminal ⁇ chain.
  • the mature C5 has a molecular weight of about 190 kDa, and consists of two polypeptide chains ( ⁇ , 115 kDa and ⁇ , 75 kDa) which are connected by disulfide bonds.
  • the C5 convertase cleaves C5 between residues 74 and 75 of the alpha chain to release the 74 amino acid C5a peptide and the C5b fragment which is subsequently incorporated into the membrane-attack complex (MAC).
  • MAC membrane-attack complex
  • Macular degeneration is a medical condition predominantly found in the elderly in which the center of the inner lining of the eye, known as the macula area of the retina, suffers thinning, atrophy, and in some cases, bleeding. This can result in loss of central vision, which entails inability to see fine details, to read, or to recognize faces.
  • Pathogenesis of new choroidal vessel formation is poorly understood, but factors such as inflammation, ischemia, and local production of angiogenic factors are thought to be important.
  • the present invention provides isolated complement C5-binding molecules (e.g., C5-binding antibodies or antigen binding fragments thereof), pharmaceutical compositions comprising such molecules, methods of making such molecules and compositions, and methods of use thereof.
  • isolated complement C5-binding molecules e.g., C5-binding antibodies or antigen binding fragments thereof
  • pharmaceutical compositions comprising such molecules, methods of making such molecules and compositions, and methods of use thereof.
  • the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibody has an affinity constant (K A ) of at least 1 ⁇ 10 7 M ⁇ 1 , 10 8 M ⁇ 1 , 10 9 M ⁇ 1 , 10 10 M ⁇ 1 , or 10 11 M ⁇ 1 .
  • K A affinity constant
  • the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, and inhibit the alternative complete pathway as measured by in vitro hemolytic assay with an IC 50 range from about 20 pM to about 200 pM.
  • the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, and cross compete with an antibody described in Table 1 below. In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that bind to the same epitope of C5 protein as an antibody described in Table 1 below.
  • the antibodies of the invention are isolated monoclonal antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention are isolated human or humanized monoclonal antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention are isolated chimeric antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention comprise a human heavy chain constant region and a human light chain constant region.
  • the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies are single chain antibodies.
  • the antibodies of the invention are Fab fragments.
  • the antibodies of the invention are scFv.
  • the present invention provides isolated antibodies or antigen-binding fragments thereof that specifically bind to both human C5 and cynomolgus C5.
  • the antibodies of the invention are an IgG isotype.
  • the present invention provides isolated antibodies or antigen binding fragments thereof comprising a framework in which amino acids have been substituted into the antibody framework from the respective human VH or VL germline sequences.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one complementarity determining (CDR) sequence having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 17, 18, 19, 20, 21, 22, 33, 34, 35, 36, 37, 38, 49, 50, 61, 62, 63, 64, 65, 66, 77, 78, 89, 95, 101, 107, 113, 119, 120, 131, 132, 133, 134, 135, 136, 145, 146, 147, 148, 149, 150, 159, 160, 161, 162, 163, 164, 173, 174, 175, 176, 177, 178, 195, 196, 197, 198, 199, 200, 209, 226, 235, 236, 237, 238, 239, or 240.
  • CDR complementarity determining
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one heavy chain CDR sequence that is identical to SEQ ID NO: 1, 2, 3, 17, 18, 19, 33, 34, 35, 49, 61, 62, 63, 77, 77, 95, 107, 113, 119, 132, 131, 133, 145, 146, 147, 159, 160, 161, 173, 174, 175, 195, 196, 197, 226, 235, 236, or 237.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one light chain CDR sequence that is identical to SEQ ID NO: 4, 5, 6, 20, 21, 22, 36, 37, 38, 50, 64, 65, 66, 78, 89, 101, 120, 134, 135, 136, 148, 149, 150, 162, 163, 164, 176, 177, 178, 198, 199, 200, 209, 238, 239, or 240.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain CDR 1 selected from the group consisting SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a heavy chain CDR2 selected from the group consisting SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; and a heavy chain CDR3 selected from the group consisting SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237.
  • a heavy chain CDR 1 selected from the group consisting SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235
  • a heavy chain CDR2 selected from the group consisting SEQ ID NOs: 2, 18, 34, 49
  • such antibodies or antigen binding fragments thereof further comprise a light chain CDR1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a light chain CDR3 selected from the group consisting SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • a light chain CDR1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238
  • a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239
  • a light chain CDR3 selected from the
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain CDR 1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a light chain CDR3 selected from the group consisting SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • a light chain CDR 1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238
  • a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 16
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281.
  • such antibodies or antigen binding fragments thereof further comprise a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, or 282.
  • such antibodies further comprise a light chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, or 290.
  • the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, or 290.
  • the present invention also comprises pharmaceutical compositions comprising one or more C5-binding molecules of the invention (e.g., C5 binding antibodies or antigen binding fragments thereof) and a pharmaceutically acceptable carrier.
  • C5-binding molecules of the invention e.g., C5 binding antibodies or antigen binding fragments thereof
  • the present invention provides nucleic acids comprising a nucleotide sequence encoding a polypeptide comprising a heavy chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281.
  • the present invention provides nucleic acids comprising a nucleotide sequence encoding a polypeptide comprising a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • the present invention also provides vectors and host cells comprising such nucleic acids.
  • the present invention provides isolated host cells comprising (1) a recombinant DNA segment encoding a heavy chain of the antibodies of the invention, and (2) a second recombinant DNA segment encoding a light chain of the antibodies of the invention; wherein said DNA segments are respectively operably linked to a first and a second promoter, and are capable of being expressed in said host cell.
  • the present invention provides isolated host cells comprising a recombinant DNA segment encoding a heavy chain, and a light chain of the antibodies of the invention, respectively, wherein said DNA segment is operably linked to a promoter, and is capable of being expressed in said host cells.
  • the host cells are non-human mammalian cell line.
  • the antibodies or antigen binding fragments thereof are a human monoclonal antibody, or an antigen binding fragment thereof.
  • the present invention further provides treatment of diagnostic methods using the C5 binding molecules (e.g., C5 binding antibodies or antigen binding fragments thereof) of the invention.
  • the present invention provides methods of treating age related macular degeneration comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or an antigen binding fragment thereof of the invention.
  • the present invention provides methods of treating a disease comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or an antigen binding fragment thereof of the invention, wherein said disease is asthma, arthritis, autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer-Simons Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, Alzheimer's disease, glomerulonephritis, or MPGN II.
  • a composition comprising an antibody or an antigen binding fragment thereof of the invention
  • the present invention also provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or antigen binding fragment thereof of the invention.
  • PNH paroxysmal nocturnal hemoglobinuria
  • the present invention further provides methods of ameliorating a symptom associated with extracorporeal circulation comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or antigen binding fragment thereof of the invention.
  • antibody as used herein includes whole antibodies and any antigen binding fragment (i.e., “antigen-binding portion”) or single chains thereof.
  • a naturally occurring “antibody” is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds.
  • Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region.
  • the heavy chain constant region is comprised of three domains, CH1, CH2 and CH3.
  • Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region.
  • the light chain constant region is comprised of one domain, CL.
  • VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR).
  • CDR complementarity determining regions
  • FR framework regions
  • Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
  • the variable regions of the heavy and light chains contain a binding domain that interacts with an antigen.
  • the constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system.
  • antigen binding portion of an antibody refers to one or more fragments of an intact antibody that retain the ability to specifically bind to a given antigen (e.g., C5).
  • Antigen binding functions of an antibody can be performed by fragments of an intact antibody.
  • binding fragments encompassed within the term “antigen binding portion” of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab) 2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; an Fd fragment consisting of the VH and CH1 domains; an Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a single domain antibody (dAb) fragment (Ward et al., 1989 Nature 341:544-546), which consists of a VH domain; and an isolated complementarity determining region (CDR).
  • Fab fragment a monovalent fragment consisting of the VL, VH, CL and CH1 domains
  • F(ab) 2 fragment a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region
  • an Fd fragment consisting of the VH and CH1 domains
  • the two domains of the Fv fragment, VL and VH are coded for by separate genes, they can be joined, using recombinant methods, by an artificial peptide linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see, e.g., Bird et al., 1988 Science 242:423-426; and Huston et al., 1988 Proc. Natl. Acad. Sci. 85:5879-5883).
  • Such single chain antibodies include one or more “antigen binding portions” of an antibody. These antibody fragments are obtained using conventional techniques known to those of skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.
  • Antigen binding portions can also be incorporated into single domain antibodies, maxibodies, minibodies, intrabodies, diabodies, triabodies, tetrabodies, v-NAR and bis-scFv (see, e.g., Hollinger and Hudson, 2005, Nature Biotechnology, 23, 9, 1126-1136).
  • Antigen binding portions of antibodies can be grafted into scaffolds based on polypeptides such as Fibronectin type III (Fn3) (see U.S. Pat. No. 6,703,199, which describes fibronectin polypeptide monobodies).
  • Fn3 Fibronectin type III
  • Antigen binding portions can be incorporated into single chain molecules comprising a pair of tandem Fv segments (VH-CH1-VH-CH1) which, together with complementary light chain polypeptides, form a pair of antigen binding regions (Zapata et al., 1995 Protein Eng. 8(10):1057-1062; and U.S. Pat. No. 5,641,870).
  • Affinity refers to the strength of interaction between antibody and antigen at single antigenic sites. Within each antigenic site, the variable region of the antibody “arm” interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the affinity.
  • the term “Avidity” refers to an informative measure of the overall stability or strength of the antibody-antigen complex. It is controlled by three major factors: antibody epitope affinity; the valency of both the antigen and antibody; and the structural arrangement of the interacting parts. Ultimately these factors define the specificity of the antibody, that is, the likelihood that the particular antibody is binding to a precise antigen epitope.
  • amino acid refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids.
  • Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, ⁇ -carboxyglutamate, and O-phosphoserine.
  • Amino acid analogs refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an alpha carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid.
  • Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid.
  • binding specificity refers to the ability of an individual antibody combining site to react with only one antigenic determinant.
  • the combining site of the antibody is located in the Fab portion of the molecule and is constructed from the hypervariable regions of the heavy and light chains. Binding affinity of an antibody is the strength of the reaction between a single antigenic determinant and a single combining site on the antibody. It is the sum of the attractive and repulsive forces operating between the antigenic determinant and the combining site of the antibody.
  • Specific binding between two entities means a binding with an equilibrium constant (K A ) of at least 1 ⁇ 10 7 M ⁇ 1 , 10 8 M ⁇ 1 , 10 9 M ⁇ 1 , 10 10 M ⁇ 1 , or 10 11 M.
  • K A equilibrium constant
  • the phrase “specifically (or selectively) binds” to an antibody refers to a binding reaction that is determinative of the presence of a cognate antigen (e.g., a human C5 or cynomolgus C5) in a heterogeneous population of proteins and other biologics.
  • an C5-binding antibody of the invention typically also has a dissociation rate constant (Kd) of about 1 ⁇ 10 ⁇ 2 s ⁇ 1 , 1 ⁇ 10 ⁇ 3 s ⁇ 1 , 1 ⁇ 10 ⁇ 4 s ⁇ 1 , 1 ⁇ 10 ⁇ 4 s ⁇ 1 , or lower, and binds to C5 with an affinity that is at least two-fold greater than its affinity for binding to a non-specific antigen (e.g., C3, C4, BSA).
  • a non-specific antigen e.g., C3, C4, BSA.
  • chimeric antibody is an antibody molecule in which (a) the constant region, or a portion thereof, is altered, replaced or exchanged so that the antigen binding site (variable region) is linked to a constant region of a different or altered class, effector function and/or species, or an entirely different molecule which confers new properties to the chimeric antibody, e.g., an enzyme, toxin, hormone, growth factor, drug, etc.; or (b) the variable region, or a portion thereof, is altered, replaced or exchanged with a variable region having a different or altered antigen specificity.
  • a mouse antibody can be modified by replacing its constant region with the constant region from a human immunoglobulin. Due to the replacement with a human constant region, the chimeric antibody can retain its specificity in recognizing the antigen while having reduced antigenicity in human as compared to the original mouse antibody.
  • C5 protein or “C5” are used interchangeably, and refers to the C5 protein in different species.
  • human C5 has the sequence as set in SEQ ID NO: 296, cynomolgus C5 has the sequence as set in SEQ ID NO: 297 ( Macaca fascicularis ) (see Table 1).
  • Human C5 can be obtained from Quidel (Cat. Number A403). Cynomolgus C5 can be produced as illustrated in the Example section below.
  • conservatively modified variant refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide.
  • nucleic acid variations are “silent variations,” which are one species of conservatively modified variations. Every nucleic acid sequence herein which encodes a polypeptide also describes every possible silent variation of the nucleic acid.
  • each codon in a nucleic acid except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan
  • TGG which is ordinarily the only codon for tryptophan
  • “conservatively modified variants” include individual substitutions, deletions or additions to a polypeptide sequence which result in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention.
  • the following eight groups contain amino acids that are conservative substitutions for one another: 1) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)).
  • the term “conservative sequence modifications” are used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence.
  • cross-block means the ability of an antibody or other binding agent to interfere with the binding of other antibodies or binding agents to C5 in a standard competitive binding assay.
  • the ability or extent to which an antibody or other binding agent is able to interfere with the binding of another antibody or binding molecule to C5, and therefore whether it can be said to cross-block according to the invention, can be determined using standard competition binding assays.
  • One suitable assay involves the use of the Biacore technology (e.g. by using the BIAcore 3000 instrument (Biacore, Uppsala, Sweden)), which can measure the extent of interactions using surface plasmon resonance technology.
  • Another assay for measuring cross-blocking uses an ELISA-based approach.
  • epitope means a protein determinant capable of specific binding to an antibody.
  • Epitopes usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.
  • high affinity for an IgG antibody refers to an antibody having a KD of 10 ⁇ 8 M or less, 10 ⁇ 9 M or less, or 10 ⁇ 10 M, or 10 ⁇ 11 M or less for a target antigen.
  • “high affinity” binding can vary for other antibody isotypes.
  • “high affinity” binding for an IgM isotype refers to an antibody having a KD of 10 ⁇ 7 M or less, or 10 ⁇ 8 M or less.
  • human antibody is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from sequences of human origin. Furthermore, if the antibody contains a constant region, the constant region also is derived from such human sequences, e.g., human germline sequences, or mutated versions of human germline sequences.
  • the human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
  • human monoclonal antibody refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences.
  • the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
  • a “humanized” antibody is an antibody that retains the reactivity of a non-human antibody while being less immunogenic in humans. This can be achieved, for instance, by retaining the non-human CDR regions and replacing the remaining parts of the antibody with their human counterparts (i.e., the constant region as well as the framework portions of the variable region). See, e.g., Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855, 1984; Morrison and Oi, Adv. Immunol., 44:65-92, 1988; Verhoeyen et al., Science, 239:1534-1536, 1988; Padlan, Molec.
  • nucleic acids or polypeptide sequences refer to two or more sequences or subsequences that are the same.
  • Two sequences are “substantially identical” if two sequences have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 60% identity, optionally 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity over a specified region, or, when not specified, over the entire sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection.
  • the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length.
  • sequence comparison typically one sequence acts as a reference sequence, to which test sequences are compared.
  • test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated.
  • sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters.
  • a “comparison window”, as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned.
  • Methods of alignment of sequences for comparison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol.
  • BLAST and BLAST 2.0 algorithms Two examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., Nuc. Acids Res. 25:3389-3402, 1977; and Altschul et al., J. Mol. Biol. 215:403-410, 1990, respectively.
  • Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra).
  • initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them.
  • the word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always ⁇ 0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached.
  • the BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment.
  • the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787, 1993).
  • One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance.
  • P(N) the smallest sum probability
  • a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001.
  • the percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4:11-17, 1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.
  • the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol, Biol.
  • nucleic acid sequences or polypeptides are substantially identical is that the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the antibodies raised against the polypeptide encoded by the second nucleic acid, as described below.
  • a polypeptide is typically substantially identical to a second polypeptide, for example, where the two peptides differ only by conservative substitutions.
  • Another indication that two nucleic acid sequences are substantially identical is that the two molecules or their complements hybridize to each other under stringent conditions, as described below.
  • Yet another indication that two nucleic acid sequences are substantially identical is that the same primers can be used to amplify the sequence.
  • isolated antibody refers to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds C5 is substantially free of antibodies that specifically bind antigens other than C5). An isolated antibody that specifically binds C5 may, however, have cross-reactivity to other antigens. Moreover, an isolated antibody may be substantially free of other cellular material and/or chemicals.
  • isotype refers to the antibody class (e.g., IgM, IgE, IgG such as IgG1 or IgG4) that is provided by the heavy chain constant region genes. Isotype also includes modified versions of one of these classes, where modifications have been made to alter the Fc function, for example, to enhance or reduce effector functions or binding to Fc receptors.
  • Kassoc or “Ka”, as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction
  • Kdis or “Kd,” as used herein, is intended to refer to the dissociation rate of a particular antibody-antigen interaction
  • K D is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/Ka) and is expressed as a molar concentration (M).
  • K D values for antibodies can be determined using methods well established in the art. A method for determining the K D of an antibody is by using surface plasmon resonance, or using a biosensor system such as a Biacore® system.
  • monoclonal antibody or “monoclonal antibody composition” as used herein refer to a preparation of antibody molecules of single molecular composition.
  • a monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.
  • nucleic acid is used herein interchangeably with the term “polynucleotide” and refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form.
  • the term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides.
  • Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs).
  • nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences, as well as the sequence explicitly indicated.
  • degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081, 1991; Ohtsuka et al., J. Biol. Chem. 260:2605-2608, 1985; and Rossolini et al., Mol. Cell. Probes 8:91-98, 1994).
  • operably linked refers to a functional relationship between two or more polynucleotide (e.g., DNA) segments. Typically, it refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence.
  • a promoter or enhancer sequence is operably linked to a coding sequence if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system.
  • promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting.
  • some transcriptional regulatory sequences, such as enhancers need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.
  • the term, “optimized” means that a nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the production cell or organism, generally a eukaryotic cell, for example, a cell of Pichia, a Chinese Hamster Ovary cell (CHO) or a human cell.
  • the optimized nucleotide sequence is engineered to retain completely or as much as possible the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the “parental” sequence.
  • the optimized sequences herein have been engineered to have codons that are preferred in mammalian cells. However, optimized expression of these sequences in other eukaryotic cells or prokaryotic cells is also envisioned herein.
  • the amino acid sequences encoded by optimized nucleotide sequences are also referred to as optimized.
  • polypeptide and “protein” are used interchangeably herein to refer to a polymer of amino acid residues.
  • the terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non-naturally occurring amino acid polymer. Unless otherwise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.
  • recombinant human antibody includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene, sequences to other DNA sequences.
  • Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences.
  • such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
  • recombinant host cell refers to a cell into which a recombinant expression vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein.
  • subject includes human and non-human animals.
  • Non-human animals include all vertebrates, e.g., mammals and non-mammals, such as non-human primates, sheep, dog, cow, chickens, amphibians, and reptiles. Except when noted, the terms “patient” or “subject” are used herein interchangeably.
  • treating includes the administration of compositions or antibodies to prevent or delay the onset of the symptoms, complications, or biochemical indicia of a disease (e.g., AMD), alleviating the symptoms or arresting or inhibiting further development of the disease, condition, or disorder.
  • Treatment may be prophylactic (to prevent or delay the onset of the disease, or to prevent the manifestation of clinical or subclinical symptoms thereof) or therapeutic suppression or alleviation of symptoms after the manifestation of the disease.
  • vector is intended to refer to a polynucleotide molecule capable of transporting another polynucleotide to which it has been linked.
  • plasmid refers to a circular double stranded DNA loop into which additional DNA segments may be ligated.
  • viral vector Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome.
  • Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors).
  • vectors e.g., non-episomal mammalian vectors
  • vectors can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome.
  • certain vectors are capable of directing the expression of genes to which they are operatively linked.
  • Such vectors are referred to herein as “recombinant expression vectors” (or simply, “expression vectors”).
  • expression vectors of utility in recombinant DNA techniques are often in the form of plasmids.
  • plasmid and vector may be used interchangeably as the plasmid is the most commonly used form of vector.
  • the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
  • FIG. 1 shows variable-region alignments of selected antibodies with their most closely related human germline sequences.
  • FIG. 2 shows a hemolytic assay in which human C5 is titrated into human C5-depleted serum to determine C5 activity.
  • FIG. 3 shows titration of cynomolgus serum into human C5-depleted serum to determine optimal cynomolgus C5 concentration for alternative pathway hemolytic assay.
  • FIG. 4 shows examples of classical pathway hemolytic assays with 20% human serum.
  • FIG. 5 shows example of alternative pathway hemolytic assays with 100 pM purified human C5 added to human C5-depleted serum.
  • FIG. 6 shows examples of alternative pathway hemolytic assays with 0.025% cynomolgus serum added to human C5-depleted serum.
  • FIG. 7 shows examples of classical pathway hemolytic assays (20% human serum) with matured Fabs in comparison to their respective parentals.
  • FIG. 8 shows examples of classical pathway hemolytic assays (5% cynomolgus serum) with matured Fabs.
  • FIG. 9 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 100 pM human C5 added to 20% human C5-depleted serum.
  • FIG. 10 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 20% human serum.
  • FIG. 11 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 100 pM cynomolgus C5 added to 20% human C5-depleted serum.
  • FIG. 12 shows characterization of germlined IgGs in classical pathway hemolytic assay using 20% human serum.
  • FIG. 13 shows characterization of germlined IgGs in classical pathway hemolytic assay using 5% cynomolgus serum.
  • FIG. 14 shows characterization of germlined IgGs in alternative pathway hemolytic assay, 100 pM human C5.
  • FIG. 15 shows characterization of final germlined IgGs in alternative pathway hemolytic assay and C5a generation ELISA using 20% human serum.
  • FIG. 16 shows affinity matured Fab characterization in the C5a ELISA using supernatant from 20% human serum hemolytic assays.
  • FIG. 17 shows specificity solution ELISA on human C3, C4, C5 and cynomolgus C5 testing antibody 7091 and its derivatives.
  • FIG. 18 shows serum stability assays (binding to human C5 in the presence of 50% serum) with the Fabs.
  • FIG. 19 shows epitope binning of some affinity improved Fabs.
  • FIG. 20 shows an ELISA for antibody binding to mouse-human chimeric C5 or human C5 to determine alpha chain versus beta chain binders.
  • C5 was presented by 5G1.1 to determine competition with 5G1.1.
  • FIG. 21 shows ELISA for testing alpha chain versus beta chain binders with 5G1.1 capture.
  • FIG. 22 shows results of hemolytic assay for testing alpha chain versus beta chain binders.
  • FIG. 23 show thermolysin proteolysis of parental Fabs at 37° C. (0, 30, 60 and 90 minutes).
  • FIG. 24 show thermolysin proteolysis of parental Fabs at 55° C. (0, 30, 60 and 90 minutes).
  • FIG. 25 shows thermolysin sensitivity of matured Fabs at 37° C.
  • FIG. 26 shows thermolysin sensitivity of matured Fabs at 55° C.
  • FIG. 27 shows examples of Fab inhibition of alternative pathway in MAC deposition assay.
  • the present invention provides antibodies that specifically bind to complement C5 protein (e.g., human C5, cynomologus C5), pharmaceutical compositions, production methods, and methods of use of such antibodies and compositions.
  • complement C5 protein e.g., human C5, cynomologus C5
  • the present invention provides antibodies that specifically bind to C5 (e.g., human C5, cynomologus C5). In some embodiments, the present invention provides antibodies that specifically bind to both human and cynomologus C5.
  • Antibodies of the invention include, but are not limited to, the human monoclonal antibodies, isolated as described, in the Examples (see Section 6 below).
  • the present invention provides antibodies that specifically bind a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VH domain having an amino acid sequence of SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281.
  • the present invention also provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VH CDR having an amino acid sequence of any one of the VH CDRs listed in Table 1, infra.
  • the invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising (or alternatively, consisting of) one, two, three, four, five or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1, infra.
  • a C5 protein e.g., human and/or cynomologus C5
  • said antibodies comprising (or alternatively, consisting of) one, two, three, four, five or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1, infra.
  • the present invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VL domain having an amino acid sequence of SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289.
  • the present invention also provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VL CDR having an amino acid sequence of any one of the VL CDRs listed in Table 1, infra.
  • the invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1, infra.
  • a C5 protein e.g., human and/or cynomologus C5
  • said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1, infra.
  • antibodies of the invention include amino acids that have been mutated, yet have at least 60, 70, 80, 90 or 95 percent identity in the CDR regions with the CDR regions depicted in the sequences described in Table 1. In some embodiments, it includes mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the CDR regions when compared with the CDR regions depicted in the sequence described in Table 1.
  • the present invention also provides nucleic acid sequences that encode VH, VL, the full length heavy chain, and the full length light chain of the antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5).
  • C5 protein e.g., human and/or cynomologus C5
  • Such nucleic acid sequences can be optimized for expression in mammalian cells (for example, Table 1 shows the optimized nucleic acid sequences for the heavy chain and light chain of antibodies 8109, 8110, 8111, 8113, 8114, 8112, 8125, 8126, 8127, 8128, 8129, 8130, 8131, 8132, and 8091).
  • antibodies of the invention include those where the amino acids or nucleic acids encoding the amino acids have been mutated, yet have at least 60, 70, 80, 90 or 95 percent identity to the sequences described in Table 1. In some embodiments, it include mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the variable regions when compared with the variable regions depicted in the sequence described in Table 1, while retaining substantially the same therapeutic activity.
  • each of these antibodies can bind to C5
  • the VH, VL, full length light chain, and full length heavy chain sequences (amino acid sequences and the nucleotide sequences encoding the amino acid sequences) can be “mixed and matched” to create other C5-binding antibodies of the invention.
  • Such “mixed and matched” C5-binding antibodies can be tested using the binding assays known in the art (e.g., ELISAs, and other assays described in the Example section).
  • ELISAs e.g., ELISAs, and other assays described in the Example section.
  • a full length heavy chain sequence from a particular full length heavy chain/full length light chain pairing should be replaced with a structurally similar full length heavy chain sequence.
  • a VL sequence from a particular VH/VL pairing should be replaced with a structurally similar VL sequence.
  • a full length light chain sequence from a particular full length heavy chain/full length light chain pairing should be replaced with a structurally similar full length light chain sequence.
  • the invention provides an isolated monoclonal antibody or antigen binding region thereof having: a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, and 281; and a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289; wherein the antibody specifically binds to C5 (e.g., human and/or cynomologus C5).
  • C5 e.g., human and/or cynomologus C5
  • the invention provides (i) an isolated monoclonal antibody having: a full length heavy chain comprising an amino acid sequence that has been optimized for expression in the cell of a mammalian selected from the group consisting of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282; and a full length light chain comprising an amino acid sequence that has been optimized for expression in the cell of a mammalian selected from the group consisting of SEQ ID NOs: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290; or (ii) a functional protein comprising an antigen binding portion thereof.
  • the present invention provides C5-binding antibodies that comprise the heavy chain and light chain CDR1s, CDR2s and CDR3s as described in Table 1, or combinations thereof.
  • the amino acid sequences of the VH CDR1s of the antibodies are shown in SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235.
  • the amino acid sequences of the VH CDR2s of the antibodies are shown in SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236.
  • the amino acid sequences of the VH CDR3s of the antibodies are shown in SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237.
  • the amino acid sequences of the VL CDR1s of the antibodies are shown in SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238.
  • the amino acid sequences of the VL CDR2s of the antibodies are shown in SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239.
  • the amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • the CDR regions are delineated using the Kabat system (Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242).
  • VH CDR1, 2 and 3 sequences and VL CDR1, 2 and 3 sequences can be “mixed and matched” (i.e., CDRs from different antibodies can be mixed and match, although each antibody must contain a VH CDR1, 2 and 3 and a VL CDR1, 2 and 3 to create other C5-binding binding molecules of the invention.
  • Such “mixed and matched” C5-binding antibodies can be tested using the binding assays known in the art and those described in the Examples (e.g., ELISAs).
  • VH CDR sequences When VH CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VH sequence should be replaced with a structurally similar CDR sequence(s).
  • VL CDR sequences when VL CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VL sequence should be replaced with a structurally similar CDR sequence(s). It will be readily apparent to the ordinarily skilled artisan that novel VH and VL sequences can be created by substituting one or more VH and/or VL CDR region sequences with structurally similar sequences from the CDR sequences shown herein for monoclonal antibodies of the present invention.
  • the present invention provides an isolated monoclonal antibody or antigen binding region thereof comprising a heavy chain variable region CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a heavy chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; a heavy chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237; a light chain variable region CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain variable region C
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO:1; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 18; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 33; a heavy chain variable region CDR2 of SEQ ID NO: 34; a heavy chain variable region CDR3 of SEQ ID NO: 35; a light chain variable region CDR1 of SEQ ID NO: 36; a light chain variable region CDR2 of SEQ ID NO: 37; and a light chain variable region CDR3 of SEQ ID NO: 38.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 50.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 66.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 78.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 95; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 113; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 113; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 119; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 120.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 131; a heavy chain variable region CDR2 of SEQ ID NO: 132; a heavy chain variable region CDR3 of SEQ ID NO: 133; a light chain variable region CDR1 of SEQ ID NO: 134; a light chain variable region CDR2 of SEQ ID NO: 135; and a light chain variable region CDR3 of SEQ ID NO: 136.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 145; a heavy chain variable region CDR2 of SEQ ID NO: 146; a heavy chain variable region CDR3 of SEQ ID NO: 147; a light chain variable region CDR1 of SEQ ID NO: 148; a light chain variable region CDR2 of SEQ ID NO: 149; and a light chain variable region CDR3 of SEQ ID NO: 150.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 159; a heavy chain variable region CDR2 of SEQ ID NO: 160; a heavy chain variable region CDR3 of SEQ ID NO: 161; a light chain variable region CDR1 of SEQ ID NO: 162; a light chain variable region CDR2 of SEQ ID NO: 163; and a light chain variable region CDR3 of SEQ ID NO: 164.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 173; a heavy chain variable region CDR2 of SEQ ID NO: 174; a heavy chain variable region CDR3 of SEQ ID NO: 175; a light chain variable region CDR1 of SEQ ID NO: 176; a light chain variable region CDR2 of SEQ ID NO: 177; and a light chain variable region CDR3 of SEQ ID NO: 178.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 195; a heavy chain variable region CDR2 of SEQ ID NO: 196; a heavy chain variable region CDR3 of SEQ ID NO: 197; a light chain variable region CDR1 of SEQ ID NO: 198; a light chain variable region CDR2 of SEQ ID NO: 199; and a light chain variable region CDR3 of SEQ ID NO: 200.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 33; a heavy chain variable region CDR2 of SEQ ID NO: 226; a heavy chain variable region CDR3 of SEQ ID NO: 35; a light chain variable region CDR1 of SEQ ID NO: 36; a light chain variable region CDR2 of SEQ ID NO: 37; and a light chain variable region CDR3 of SEQ ID NO: 38.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 235; a heavy chain variable region CDR2 of SEQ ID NO: 236; a heavy chain variable region CDR3 of SEQ ID NO: 237; a light chain variable region CDR1 of SEQ ID NO: 238; a light chain variable region CDR2 of SEQ ID NO: 239; and a light chain variable region CDR3 of SEQ ID NO: 240.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 119; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 120.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 95; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 66.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 78.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89.
  • an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • an antibody that specifically binds to C5 is an antibody that is described in Table 1.
  • a human antibody comprises heavy or light chain variable regions or full length heavy or light chains that are “the product of” or “derived from” a particular germline sequence if the variable regions or full length chains of the antibody are obtained from a system that uses human germline immunoglobulin genes.
  • Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest.
  • a human antibody that is “the product of” or “derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody.
  • a human antibody that is “the product of” or “derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally occurring somatic mutations or intentional introduction of site-directed mutations.
  • a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences).
  • a human antibody may be at least 60%, 70%, 80%, 90%, or at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene.
  • a recombinant human antibody will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene in the VH or VL framework regions. In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene.
  • the present invention provides an antibody or an antigen-binding fragment thereof comprising amino acid sequences that are homologous to the sequences described in Table 1, and said antibody binds to a C5 protein (e.g., human and/or cynomologus C5), and retains the desired functional properties of those antibodies described in Table 1.
  • a C5 protein e.g., human and/or cynomologus C5
  • the invention provides an isolated monoclonal antibody (or a functional antigen binding fragment thereof) comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281; the light chain variable region comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 2
  • the VH and/or VL amino acid sequences may be 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1. In other embodiments, the VH and/or VL amino acid sequences may be identical except an amino acid substitution in no more than 1, 2, 3, 4 or 5 amino acid position.
  • An antibody having VH and VL regions having high (i.e., 80% or greater) identity to the VH and VL regions of those described in Table 1 can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281; and 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289 respectively, followed by testing of the encoded altered antibody for retained function using the functional assays described herein.
  • mutagenesis e.g., site-directed or PCR-mediated mutagenesis
  • the full length heavy chain and/or full length light chain amino acid sequences may be 50% 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1.
  • the full length heavy chain and/or full length light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth above.
  • variable regions of heavy chain and/or light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth above
  • the percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity equals number of identical positions/total number of positions ⁇ 100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences.
  • the comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as described in the non-limiting examples below.
  • the protein sequences of the present invention can further be used as a “query sequence” to perform a search against public databases to, for example, identify related sequences.
  • search can be performed using the BLAST program (version 2.0) of Altschul, et al., 1990 J. Mol. Biol. 215:403-10.
  • an antibody of the invention has a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein one or more of these CDR sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the C5-binding antibodies of the invention.
  • the invention provides an isolated monoclonal antibody, or a functional antigen binding fragment thereof, consisting of a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein: the heavy chain variable region CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235, and conservative modifications thereof; the heavy chain variable region CDR2 amino acid sequences are selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, and conservative modifications thereof; the heavy chain variable region CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237, and conservative modifications thereof
  • an antibody of the invention optimized for expression in a mammalian cell has a full length heavy chain sequence and a full length light chain sequence, wherein one or more of these sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the C5-binding antibodies of the invention.
  • the invention provides an isolated monoclonal antibody optimized for expression in a mammalian cell consisting of a full length heavy chain and a full length light chain wherein: the full length heavy chain has amino acid sequences selected from the group of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282, and conservative modifications thereof; and the full length light chain has amino acid sequences selected from the group of SEQ ID NOs: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290, and conservative modifications thereof; the antibody specifically binds to C5 (e.g., human and/or cynomologus C5); and the antibody
  • the present invention provides antibodies that bind to the same epitope as do the C5-binding antibodies described in Table 1. Additional antibodies can therefore be identified based on their ability to cross-compete (e.g., to competitively inhibit the binding of, in a statistically significant manner) with other antibodies of the invention in C5 binding assays.
  • test antibody to inhibit the binding of antibodies of the present invention to a C5 protein (e.g., human and/or cynomolgus C5) demonstrates that the test antibody can compete with that antibody for binding to C5; such an antibody may, according to non-limiting theory, bind to the same or a related (e.g., a structurally similar or spatially proximal) epitope on the C5 protein as the antibody with which it competes.
  • the antibody that binds to the same epitope on C5 as the antibodies of the present invention is a human monoclonal antibody.
  • Such human monoclonal antibodies can be prepared and isolated as described herein.
  • An antibody of the invention further can be prepared using an antibody having one or more of the VH and/or VL sequences shown herein as starting material to engineer a modified antibody, which modified antibody may have altered properties from the starting antibody.
  • An antibody can be engineered by modifying one or more residues within one or both variable regions (i.e., VH and/or VL), for example within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be engineered by modifying residues within the constant region(s), for example to alter the effector function(s) of the antibody.
  • CDR grafting One type of variable region engineering that can be performed is CDR grafting.
  • Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann, L. et al., 1998 Nature 332:323-327; Jones, P.
  • another embodiment of the invention pertains to an isolated monoclonal antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region comprising CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; CDR3 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237, respectively; and a light chain variable region having CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238;
  • Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences.
  • germline DNA sequences for human heavy and light chain variable region genes can be found in the “VBase” human germline sequence database (available on the Internet at www.mrc-cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Tomlinson, I. M., et al., 1992 J. fol. Biol. 227:776-798; and Cox, J. P. L. et al., 1994 Eur. J Immunol. 24:827-836; the contents of each of which are expressly incorporated herein by reference.
  • framework sequences for use in the antibodies of the invention are those that are structurally similar to the framework sequences used by selected antibodies of the invention, e.g., consensus sequences and/or framework sequences used by monoclonal antibodies of the invention.
  • the VH CDR1, 2 and 3 sequences, and the VL CDR1, 2 and 3 sequences can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences.
  • variable region modification is to mutate amino acid residues within the VH and/or VL CDR1, CDR2 and/or CDR3 regions to thereby improve one or more binding properties (e.g., affinity) of the antibody of interest, known as “affinity maturation.”
  • Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples.
  • Conservative modifications (as discussed above) can be introduced.
  • the mutations may be amino acid substitutions, additions or deletions. Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered.
  • the invention provides isolated C5-binding monoclonal antibodies, or an antigen binding fragment thereof, consisting of a heavy chain variable region having: a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a VH CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 2,
  • antibody/immunoglobulin frameworks or scaffolds can be employed so long as the resulting polypeptide includes at least one binding region which specifically binds to C5.
  • Such frameworks or scaffolds include the 5 main idiotypes of human immunoglobulins, or fragments thereof, and include immunoglobulins of other animal species, preferably having humanized aspects. Single heavy-chain antibodies such as those identified in camelids are of particular interest in this regard. Novel frameworks, scaffolds and fragments continue to be discovered and developed by those skilled in the art.
  • the invention pertains to generating non-immunoglobulin based antibodies using non-immunoglobulin scaffolds onto which CDRs of the invention can be grafted.
  • Known or future non-immunoglobulin frameworks and scaffolds may be employed, as long as they comprise a binding region specific for the target C5 protein (e.g., human and/or cynomolgus C5).
  • Non-immunoglobulin frameworks or scaffolds include, but are not limited to, fibronectin (Compound Therapeutics, Inc., Waltham, Mass.), ankyrin (Molecular Partners AG, Zurich, Switzerland), domain antibodies (Domantis, Ltd., Cambridge, Mass., and Ablynx nv, Zwijnaarde, Belgium), lipocalin (Pieris Proteolab AG, Freising, Germany), small modular immuno-pharmaceuticals (Trubion Pharmaceuticals Inc., Seattle, Wash.), maxybodies (Avidia, Inc., Mountain View, Calif.), Protein A (Affibody AG, Sweden), and affilin (gamma-crystallin or ubiquitin) (Scil Proteins GmbH, Halle, Germany).
  • fibronectin Compound Therapeutics, Inc., Waltham, Mass.
  • ankyrin Molecular Partners AG, Zurich, Switzerland
  • domain antibodies Domantis, Ltd., Cambridge, Mass., and Ablynx nv
  • the fibronectin scaffolds are based on fibronectin type III domain (e.g., the tenth module of the fibronectin type III (10 Fn3 domain)).
  • the fibronectin type III domain has 7 or 8 beta strands which are distributed between two beta sheets, which themselves pack against each other to form the core of the protein, and further containing loops (analogous to CDRs) which connect the beta strands to each other and are solvent exposed. There are at least three such loops at each edge of the beta sheet sandwich, where the edge is the boundary of the protein perpendicular to the direction of the beta strands (see U.S. Pat. No. 6,818,418).
  • fibronectin-based scaffolds are not an immunoglobulin, although the overall fold is closely related to that of the smallest functional antibody fragment, the variable region of the heavy chain, which comprises the entire antigen recognition unit in camel and llama IgG. Because of this structure, the non-immunoglobulin antibody mimics antigen binding properties that are similar in nature and affinity to those of antibodies.
  • These scaffolds can be used in a loop randomization and shuffling strategy in vitro that is similar to the process of affinity maturation of antibodies in vivo.
  • These fibronectin-based molecules can be used as scaffolds where the loop regions of the molecule can be replaced with CDRs of the invention using standard cloning techniques.
  • the ankyrin technology is based on using proteins with ankyrin derived repeat modules as scaffolds for bearing variable regions which can be used for binding to different targets.
  • the ankyrin repeat module is a 33 amino acid polypeptide consisting of two anti-parallel ⁇ -helices and a ⁇ -turn. Binding of the variable regions is mostly optimized by using ribosome display.
  • Avimers are derived from natural A-domain containing protein such as LRP-1. These domains are used by nature for protein-protein interactions and in human over 250 proteins are structurally based on A-domains. Avimers consist of a number of different “A-domain” monomers (2-10) linked via amino acid linkers. Avimers can be created that can bind to the target antigen using the methodology described in, for example, U.S. Patent Application Publication Nos. 20040175756; 20050053973; 20050048512; and 20060008844.
  • Affibody affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A.
  • Protein A is a surface protein from the bacterium Staphylococcus aureus .
  • This scaffold domain consists of 58 amino acids, 13 of which are randomized to generate affibody libraries with a large number of ligand variants (See e.g., U.S. Pat. No. 5,831,012).
  • Affibody molecules mimic antibodies, they have a molecular weight of 6 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of affibody molecules is similar to that of an antibody.
  • Anticalins are products developed by the company Pieris ProteoLab AG. They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the physiological transport or storage of chemically sensitive or insoluble compounds. Several natural lipocalins occur in human tissues or body liquids. The protein architecture is reminiscent of immunoglobulins, with hypervariable loops on top of a rigid framework. However, in contrast with antibodies or their recombinant fragments, lipocalins are composed of a single polypeptide chain with 160 to 180 amino acid residues, being just marginally bigger than a single immunoglobulin domain. The set of four loops, which makes up the binding pocket, shows pronounced structural plasticity and tolerates a variety of side chains.
  • the binding site can thus be reshaped in a proprietary process in order to recognize prescribed target molecules of different shape with high affinity and specificity.
  • One protein of lipocalin family the bilin-binding protein (BBP) of Pieris Brassicae has been used to develop anticalins by mutagenizing the set of four loops.
  • BBP bilin-binding protein
  • One example of a patent application describing anticalins is in PCT Publication No. WO 199916873.
  • Affilin molecules are small non-immunoglobulin proteins which are designed for specific affinities towards proteins and small molecules.
  • New affilin molecules can be very quickly selected from two libraries, each of which is based on a different human derived scaffold protein. Affilin molecules do not show any structural homology to immunoglobulin proteins.
  • two affilin scaffolds are employed, one of which is gamma crystalline, a human structural eye lens protein and the other is “ubiquitin” superfamily proteins. Both human scaffolds are very small, show high temperature stability and are almost resistant to pH changes and denaturing agents. This high stability is mainly due to the expanded beta sheet structure of the proteins. Examples of gamma crystalline derived proteins are described in WO200104144 and examples of “ubiquitin-like” proteins are described in WO2004106368.
  • PEM Protein epitope mimetics
  • the Fabs are converted to silent IgG1 format by changing the Fc region.
  • antibodies 6525-7910 in Table 1 can be converted to silent IgG1 formate by substituting the “X” in the amino acid sequences for the heavy chain with: CDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCWVDVSHEDPEVKFNWYVD GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKG QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSPGK (SEQ ID NO: 293) and substituting the “X” in the amino acid sequence for the light chain with: CS if the light chain is lambda, or C if the light chain is kappa
  • a “silent IgG1” is an IgG1 Fc sequence in which the amino acid sequence has been altered to reduce Fc-mediated effector functions (for example ADCC and/or CDC). Such an antibody will typically have reduced binding to Fc receptors and/or C1q.
  • the Fabs are converted to IgG2 format.
  • antibodies 6525-7910 in Table 1 can be converted to IgG2 format by substituting the constant sequence ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX (SEQ ID NO: 294)
  • the present invention provides fully human antibodies that specifically bind to a C5 protein (e.g., human and/or cynomolgus C5). Compared to the chimeric or humanized antibodies, the human C5-binding antibodies of the invention have further reduced antigenicity when administered to human subjects.
  • a C5 protein e.g., human and/or cynomolgus C5
  • the human C5-binding antibodies of the invention have further reduced antigenicity when administered to human subjects.
  • the human C5-binding antibodies can be generated using methods that are known in the art. For example, the humaneering technology used to converting non-human antibodies into engineered human antibodies.
  • U.S. Patent Publication No. 20050008625 describes an in vivo method for replacing a nonhuman antibody variable region with a human variable region in an antibody while maintaining the same or providing better binding characteristics relative to that of the nonhuman antibody. The method relies on epitope guided replacement of variable regions of a non-human reference antibody with a fully human antibody. The resulting human antibody is generally unrelated structurally to the reference nonhuman antibody, but binds to the same epitope on the same antigen as the reference antibody.
  • the serial epitope-guided complementarity replacement approach is enabled by setting up a competition in cells between a “competitor” and a library of diverse hybrids of the reference antibody (“test antibodies”) for binding to limiting amounts of antigen in the presence of a reporter system which responds to the binding of test antibody to antigen.
  • the competitor can be the reference antibody or derivative thereof such as a single-chain Fv fragment.
  • the competitor can also be a natural or artificial ligand of the antigen which binds to the same epitope as the reference antibody.
  • the only requirements of the competitor are that it binds to the same epitope as the reference antibody, and that it competes with the reference antibody for antigen binding.
  • test antibodies have one antigen-binding V-region in common from the nonhuman reference antibody, and the other V-region selected at random from a diverse source such as a repertoire library of human antibodies.
  • the common V-region from the reference antibody serves as a guide, positioning the test antibodies on the same epitope on the antigen, and in the same orientation, so that selection is biased toward the highest antigen-binding fidelity to the reference antibody.
  • reporter system can be used to detect desired interactions between test antibodies and antigen.
  • complementing reporter fragments may be linked to antigen and test antibody, respectively, so that reporter activation by fragment complementation only occurs when the test antibody binds to the antigen.
  • reporter activation becomes dependent on the ability of the test antibody to compete with the competitor, which is proportional to the affinity of the test antibody for the antigen.
  • Other reporter systems include the reactivator of an auto-inhibited reporter reactivation system (RAIR) as disclosed in U.S. patent application Ser. No. 10/208,730 (Publication No. 20030198971), or competitive activation system disclosed in U.S. patent application Ser. No. 10/076,845 (Publication No. 20030157579).
  • RAIR auto-inhibited reporter reactivation system
  • test antibodies are initially selected on the basis of their activity relative to that of the reference antibody when expressed as the test antibody.
  • the result of the first round of selection is a set of “hybrid” antibodies, each of which is comprised of the same non-human V-region from the reference antibody and a human V-region from the library, and each of which binds to the same epitope on the antigen as the reference antibody.
  • One of more of the hybrid antibodies selected in the first round will have an affinity for the antigen comparable to or higher than that of the reference antibody.
  • the human V-regions selected in the first step are used as guide for the selection of human replacements for the remaining non-human reference antibody V-region with a diverse library of cognate human V-regions.
  • the hybrid antibodies selected in the first round may also be used as competitors for the second round of selection.
  • the result of the second round of selection is a set of fully human antibodies which differ structurally from the reference antibody, but which compete with the reference antibody for binding to the same antigen.
  • Some of the selected human antibodies bind to the same epitope on the same antigen as the reference antibody.
  • one or more binds to the same epitope with an affinity which is comparable to or higher than that of the reference antibody.
  • mouse or chimeric C5-binding antibodies described above as the reference antibody, this method can be readily employed to generate human antibodies that bind to human C5 with the same binding specificity and the same or better binding affinity.
  • human C5-binding antibodies can also be commercially obtained from companies which customarily produce human antibodies, e.g., KaloBios, Inc. (Mountain View, Calif.).
  • Certain IgG antibodies from this family of mammals as found in nature lack light chains, and are thus structurally distinct from the typical four chain quaternary structure having two heavy and two light chains, for antibodies from other animals. See PCT/EP93/02214 (WO 94/04678 published 3 Mar. 1994).
  • a region of the camelid antibody which is the small single variable domain identified as VHH can be obtained by genetic engineering to yield a small protein having high affinity for a target, resulting in a low molecular weight antibody-derived protein known as a “camelid nanobody”.
  • VHH camelid antibody
  • the camelid nanobody has a molecular weight approximately one-tenth that of a human IgG molecule, and the protein has a physical diameter of only a few nanometers.
  • One consequence of the small size is the ability of camelid nanobodies to bind to antigenic sites that are functionally invisible to larger antibody proteins, i.e., camelid nanobodies are useful as reagents detect antigens that are otherwise cryptic using classical immunological techniques, and as possible therapeutic agents.
  • a camelid nanobody can inhibit as a result of binding to a specific site in a groove or narrow cleft of a target protein, and hence can serve in a capacity that more closely resembles the function of a classical low molecular weight drug than that of a classical antibody.
  • camelid nanobodies being extremely thermostable, stable to extreme pH and to proteolytic digestion, and poorly antigenic. Another consequence is that camelid nanobodies readily move from the circulatory system into tissues, and even cross the blood-brain barrier and can treat disorders that affect nervous tissue. Nanobodies can further facilitated drug transport across the blood brain barrier. See U.S. patent application 20040161738 published Aug. 19, 2004. These features combined with the low antigenicity to humans indicate great therapeutic potential. Further, these molecules can be fully expressed in prokaryotic cells such as E. coli and are expressed as fusion proteins with bacteriophage and are functional.
  • a feature of the present invention is a camelid antibody or nanobody having high affinity for C5.
  • the camelid antibody or nanobody is naturally produced in the camelid animal, i.e., is produced by the camelid following immunization with C5 or a peptide fragment thereof, using techniques described herein for other antibodies.
  • the C5-binding camelid nanobody is engineered, i.e., produced by selection for example from a library of phage displaying appropriately mutagenized camelid nanobody proteins using panning procedures with C5 as a target as described in the examples herein.
  • Engineered nanobodies can further be customized by genetic engineering to have a half life in a recipient subject of from 45 minutes to two weeks.
  • the camelid antibody or nanobody is obtained by grafting the CDRs sequences of the heavy or light chain of the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in PCT/EP93/02214.
  • the present invention features bispecific or multispecific molecules comprising an C5-binding antibody, or a fragment thereof, of the invention.
  • An antibody of the invention, or antigen-binding regions thereof can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules.
  • the antibody of the invention may in fact be derivatized or linked to more than one other functional molecule to generate multi-specific molecules that bind to more than two different binding sites and/or target molecules; such multi-specific molecules are also intended to be encompassed by the term “bispecific molecule” as used herein.
  • an antibody of the invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results.
  • the present invention includes bispecific molecules comprising at least one first binding specificity for C5 and a second binding specificity for a second target epitope.
  • the second target epitope is another epitope of C5 different from the first target epitope.
  • the molecule can further include a third binding specificity, in addition to the first and second target epitope.
  • the bispecific molecules of the invention comprise as a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g., an Fab, Fab′, F(ab′)2, Fv, or a single chain Fv.
  • the antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as described in Ladner et al. U.S. Pat. No. 4,946,778.
  • Diabodies are bivalent, bispecific molecules in which VH and VL domains are expressed on a single polypeptide chain, connected by a linker that is too short to allow for pairing between the two domains on the same chain.
  • the VH and VL domains pair with complementary domains of another chain, thereby creating two antigen binding sites (see e.g., Holliger et al., 1993 Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak et al., 1994 Structure 2:1121-1123).
  • Diabodies can be produced by expressing two polypeptide chains with either the structure VHA-VLB and VHB-VLA (VH-VL configuration), or VLA-VHB and VLB-VHA (VL-VH configuration) within the same cell. Most of them can be expressed in soluble form in bacteria.
  • Single chain diabodies (scDb) are produced by connecting the two diabody-forming polypeptide chains with linker of approximately 15 amino acid residues (see Holliger and Winter, 1997 Cancer Immunol. Immunother., 45(3-4):128-30; Wu et al., 1996 Immunotechnology, 2(1):21-36).
  • scDb can be expressed in bacteria in soluble, active monomeric form (see Holliger and Winter, 1997 Cancer Immunol.
  • a diabody can be fused to Fc to generate a “di-diabody” (see Lu et al., 2004 J. Biol. Chem., 279(4):2856-65).
  • antibodies which can be employed in the bispecific molecules of the invention are murine, chimeric and humanized monoclonal antibodies.
  • the bispecific molecules of the present invention can be prepared by conjugating the constituent binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross-linking agents can be used for covalent conjugation.
  • cross-linking agents examples include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohaxane-I-carboxylate (sulfo-SMCC) (see e.g., Karpovsky et al., 1984 J. Exp. Med.
  • Conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, Ill.).
  • the binding specificities are antibodies, they can be conjugated by sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains.
  • the hinge region is modified to contain an odd number of sulfhydryl residues, for example one, prior to conjugation.
  • both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell.
  • This method is particularly useful where the bispecific molecule is a mAb ⁇ mAb, mAb ⁇ Fab, Fab ⁇ F(ab′)2 or ligand ⁇ Fab fusion protein.
  • a bispecific molecule of the invention can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants.
  • Bispecific molecules may comprise at least two single chain molecules. Methods for preparing bispecific molecules are described for example in U.S. Pat. No. 5,260,203; U.S. Pat. No. 5,455,030; U.S. Pat. No. 4,881,175; U.S. Pat.
  • Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (REA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay.
  • ELISA enzyme-linked immunosorbent assay
  • REA radioimmunoassay
  • FACS analysis FACS analysis
  • bioassay e.g., growth inhibition
  • Western Blot assay Western Blot assay.
  • Each of these assays generally detects the presence of protein-antibody complexes of particular interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest.
  • the present invention provides multivalent compounds comprising at least two identical or different antigen-binding portions of the antibodies of the invention binding to C5.
  • the antigen-binding portions can be linked together via protein fusion or covalent or non covalent linkage.
  • methods of linkage has been described for the bispecfic molecules.
  • Tetravalent compounds can be obtained for example by cross-linking antibodies of the antibodies of the invention with an antibody that binds to the constant regions of the antibodies of the invention, for example the Fc or hinge region.
  • Trimerizing domain are described for example in Borean patent EP 1 012 280B1. Pentamerizing modules are described for example in PCT/EP97/05897.
  • the present invention provides for antibodies that specifically bind to C5 protein which have an extended half-life in vivo.
  • kidney filtration kidney filtration, metabolism in the liver, degradation by proteolytic enzymes (proteases), and immunogenic responses (e.g., protein neutralization by antibodies and uptake by macrophages and dentritic cells).
  • proteolytic enzymes proteolytic enzymes
  • immunogenic responses e.g., protein neutralization by antibodies and uptake by macrophages and dentritic cells.
  • a variety of strategies can be used to extend the half life of the antibodies of the present invention.
  • polyethyleneglycol PEG
  • PEG polyethyleneglycol
  • PSA polysialic acid
  • HES hydroxyethyl starch
  • albumin-binding ligands and carbohydrate shields
  • genetic fusion to proteins binding to serum proteins such as albumin, IgG, FcRn, and transferring
  • other binding moieties that bind to serum proteins, such as nanobodies, Fabs, DARPins, avimers, affibodies, and anticalins
  • genetic fusion to rPEG, albumin, domain of albumin, albumin-binding proteins, and Fc or by incorporation into nancarriers, slow release formulations, or medical devices.
  • inert polymer molecules such as high molecular weight PEG can be attached to the antibodies or a fragment thereof with or without a multifunctional linker either through site-specific conjugation of the PEG to the N- or C-terminus of the antibodies or via epsilon-amino groups present on lysine residues.
  • PEG polyethylene glycol
  • the antibody, or fragment thereof typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment.
  • the pegylation can be carried out by an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer).
  • a reactive PEG molecule or an analogous reactive water-soluble polymer.
  • polyethylene glycol is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (C1-C10) alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol-maleimide.
  • the antibody to be pegylated is an aglycosylated antibody. Linear or branched polymer derivatization that results in minimal loss of biological activity will be used.
  • the degree of conjugation can be closely monitored by SDS-PAGE and mass spectrometry to ensure proper conjugation of PEG molecules to the antibodies. Unreacted PEG can be separated from antibody-PEG conjugates by size-exclusion or by ion-exchange chromatography. PEG-derivatized antibodies can be tested for binding activity as well as for in vivo efficacy using methods well-known to those of skill in the art, for example, by immunoassays described herein. Methods for pegylating proteins are known in the art and can be applied to the antibodies of the invention. See for example, EP 0 154 316 by Nishimura et al. and EP 0 401 384 by Ishikawa et al.
  • modified pegylation technologies include reconstituting chemically orthogonal directed engineering technology (ReCODE PEG), which incorporates chemically specified side chains into biosynthetic proteins via a reconstituted system that includes tRNA synthetase and tRNA.
  • ReCODE PEG chemically orthogonal directed engineering technology
  • This technology enables incorporation of more than 30 new amino acids into biosynthetic proteins in E. coli , yeast, and mammalian cells.
  • the tRNA incorporates a nonnative amino acid any place an amber codon is positioned, converting the amber from a stop codon to one that signals incorporation of the chemically specified amino acid.
  • Recombinant pegylation technology can also be used for serum halflife extension.
  • This technology involves genetically fusing a 300-600 amino acid unstructured protein tail to an existing pharmaceutical protein. Because the apparent molecular weight of such an unstructured protein chain is about 15-fold larger than its actual molecular weight, the serum halflife of the protein is greatly increased. In contrast to traditional PEGylation, which requires chemical conjugation and repurification, the manufacturing process is greatly simplified and the product is homogeneous.
  • PSA polymer polysialic acid
  • PSA is a polymer of sialic acid (a sugar).
  • sialic acid a sugar
  • polysialic acid provides a protective microenvironment on conjugation. This increases the active life of the therapeutic protein in the circulation and prevents it from being recognized by the immune system.
  • the PSA polymer is naturally found in the human body. It was adopted by certain bacteria which evolved over millions of years to coat their walls with it. These naturally polysialylated bacteria were then able, by virtue of molecular mimicry, to foil the body's defense system. PSA, nature's ultimate stealth technology, can be easily produced from such bacteria in large quantities and with predetermined physical characteristics. Bacterial PSA is completely non-immunogenic, even when coupled to proteins, as it is chemically identical to PSA in the human body.
  • HES hydroxyethyl starch
  • Another technology include the use of hydroxyethyl starch (“HES”) derivatives linked to antibodies.
  • HES is a modified natural polymer derived from waxy maize starch and can be metabolized by the body's enzymes.
  • HES solutions are usually administered to substitute deficient blood volume and to improve the rheological properties of the blood. Hesylation of an antibody enables the prolongation of the circulation half-life by increasing the stability of the molecule, as well as by reducing renal clearance, resulting in an increased biological activity.
  • a wide range of HES antibody conjugates can be customized.
  • Antibodies having an increased half-life in vivo can also be generated introducing one or more amino acid modifications (i.e., substitutions, insertions or deletions) into an IgG constant domain, or FcRn binding fragment thereof (preferably a Fc or hinge Fc domain fragment). See, e.g., International Publication No. WO 98/23289; International Publication No. WO 97/34631; and U.S. Pat. No. 6,277,375.
  • antibodies can be conjugated to albumin in order to make the antibody or antibody fragment more stable in vivo or have a longer half life in vivo.
  • the techniques are well-known in the art, see, e.g., International Publication Nos. WO 93/15199, WO 93/15200, and WO 01/77137; and European Patent No. EP 413,622.
  • the strategies for increasing half life is especially useful in nanobodies, fibronectin-based binders, and other antibodies or proteins for which increased in vivo half life is desired.
  • the present invention provides antibodies or fragments thereof that specifically bind to a C5 protein recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to a heterologous protein or polypeptide (or fragment thereof, preferably to a polypeptide of at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90 or at least 100 amino acids) to generate fusion proteins.
  • the invention provides fusion proteins comprising an antigen-binding fragment of an antibody described herein (e.g., a Fab fragment, Fd fragment, Fv fragment, F(ab)2 fragment, a VH domain, a VH CDR, a VL domain or a VL CDR) and a heterologous protein, polypeptide, or peptide.
  • an antibody described herein e.g., a Fab fragment, Fd fragment, Fv fragment, F(ab)2 fragment, a VH domain, a VH CDR, a VL domain or a VL CDR
  • Methods for fusing or conjugating proteins, polypeptides, or peptides to an antibody or an antibody fragment are known in the art. See, e.g., U.S. Pat. Nos. 5,336,603, 5,622,929, 5,359,046, 5,349,053, 5,447,851, and 5,112,946; European Patent Nos.
  • EP 307,434 and EP 367,166 International Publication Nos. WO 96/04388 and WO 91/06570; Ashkenazi et al., 1991, Proc. Natl. Acad. Sci. USA 88: 10535-10539; Zheng et al., 1995, J. Immunol. 154:5590-5600; and Vil et al., 1992, Proc. Natl. Acad. Sci. USA 89:11337-11341.
  • DNA shuffling may be employed to alter the activities of antibodies of the invention or fragments thereof (e.g., antibodies or fragments thereof with higher affinities and lower dissociation rates). See, generally, U.S. Pat. Nos. 5,605,793, 5,811,238, 5,830,721, 5,834,252, and 5,837,458; Patten et al., 1997, Curr. Opinion Biotechnol. 8:724-33; Harayama, 1998, Trends Biotechnol.
  • Antibodies or fragments thereof, or the encoded antibodies or fragments thereof may be altered by being subjected to random mutagenesis by error-prone PCR, random nucleotide insertion or other methods prior to recombination.
  • a polynucleotide encoding an antibody or fragment thereof that specifically binds to a C5 protein may be recombined with one or more components, motifs, sections, parts, domains, fragments, etc. of one or more heterologous molecules.
  • the antibodies or fragments thereof can be fused to marker sequences, such as a peptide to facilitate purification.
  • the marker amino acid sequence is a hexa-histidine peptide, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, Calif., 91311), among others, many of which are commercially available.
  • hexa-histidine provides for convenient purification of the fusion protein.
  • peptide tags useful for purification include, but are not limited to, the hemagglutinin (“HA”) tag, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., 1984, Cell 37:767), and the “flag” tag.
  • HA hemagglutinin
  • antibodies of the present invention or fragments thereof conjugated to a diagnostic or detectable agent can be useful for monitoring or prognosing the onset, development, progression and/or severity of a disease or disorder as part of a clinical testing procedure, such as determining the efficacy of a particular therapy.
  • Such diagnosis and detection can accomplished by coupling the antibody to detectable substances including, but not limited to, various enzymes, such as, but not limited to, horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; prosthetic groups, such as, but not limited to, streptavidinlbiotin and avidin/biotin; fluorescent materials, such as, but not limited to, umbelliferone, fluorescein, fluorescein isothiocynate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; luminescent materials, such as, but not limited to, luminol; bioluminescent materials, such as but not limited to, luciferase, luciferin, and aequorin; radioactive materials, such as, but not limited to, iodine (131I, 125I, 123I, and 121
  • the present invention further encompasses uses of antibodies or fragments thereof conjugated to a therapeutic moiety.
  • An antibody or fragment thereof may be conjugated to a therapeutic moiety such as a cytotoxin, e.g., a cytostatic or cytocidal agent, a therapeutic agent or a radioactive metal ion, e.g., alpha-emitters.
  • a cytotoxin or cytotoxic agent includes any agent that is detrimental to cells.
  • an antibody or fragment thereof may be conjugated to a therapeutic moiety or drug moiety that modifies a given biological response.
  • Therapeutic moieties or drug moieties are not to be construed as limited to classical chemical therapeutic agents.
  • the drug moiety may be a protein, peptide, or polypeptide possessing a desired biological activity.
  • Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria toxin; a protein such as tumor necrosis factor, ⁇ -interferon, ⁇ -interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator, an apoptotic agent, an anti-angiogenic agent; or, a biological response modifier such as, for example, a lymphokine.
  • a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria toxin
  • a protein such as tumor necrosis factor, ⁇ -interferon, ⁇ -interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator, an apoptotic agent, an anti-angiogenic agent
  • a biological response modifier such as, for example, a lymphokine.
  • an antibody can be conjugated to therapeutic moieties such as a radioactive metal ion, such as alph-emiters such as 213Bi or macrocyclic chelators useful for conjugating radiometal ions, including but not limited to, 131In, 131LU, 131Y, 131Ho, 131Sm, to polypeptides.
  • the macrocyclic chelator is 1,4,7,10-tetraazacyclododecane-N,N′,N′′,N′′′-tetraacetic acid (DOTA) which can be attached to the antibody via a linker molecule.
  • linker molecules are commonly known in the art and described in Denardo et al., 1998, Clin Cancer Res.
  • Antibodies may also be attached to solid supports, which are particularly useful for immunoassays or purification of the target antigen.
  • solid supports include, but are not limited to, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride or polypropylene.
  • the invention provides substantially purified nucleic acid molecules which encode polypeptides comprising segments or domains of the C5-binding antibody chains described above.
  • Some of the nucleic acids of the invention comprise the nucleotide sequence encoding the heavy chain variable region shown in SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281, and/or the nucleotide sequence encoding the light chain variable region shown in SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289.
  • nucleic acid molecules are those identified in Table 1.
  • Some other nucleic acid molecules of the invention comprise nucleotide sequences that are substantially identical (e.g., at least 65, 80%, 95%, or 99%) to the nucleotide sequences of those identified in Table 1.
  • polypeptides encoded by these polynucleotides are capable of exhibiting C5 antigen binding capacity.
  • polynucleotides which encode at least one CDR region and usually all three CDR regions from the heavy or light chain of the C5-binding antibody set forth above. Some other polynucleotides encode all or substantially all of the variable region sequence of the heavy chain and/or the light chain of the C5-binding antibody set forth above. Because of the degeneracy of the code, a variety of nucleic acid sequences will encode each of the immunoglobulin amino acid sequences.
  • nucleic acid molecules of the invention can encode both a variable region and a constant region of the antibody.
  • Some of nucleic acid sequences of the invention comprise nucleotides encoding a mature heavy chain variable region sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature heavy chain variable region sequence set forth in SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281.
  • nucleic acid sequences comprising nucleotide encoding a mature light chain variable region sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature light chain variable region sequence set forth in SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289.
  • the polynucleotide sequences can be produced by de novo solid-phase DNA synthesis or by PCR mutagenesis of an existing sequence (e.g., sequences as described in the Examples below) encoding an C5-binding antibody or its binding fragment.
  • Direct chemical synthesis of nucleic acids can be accomplished by methods known in the art, such as the phosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90; the phosphodiester method of Brown et al., Meth. Enzymol. 68:109, 1979; the diethylphosphoramidite method of Beaucage et al., Tetra. Lett., 22:1859, 1981; and the solid support method of U.S. Pat. No.
  • Nonviral vectors and systems include plasmids, episomal vectors, typically with an expression cassette for expressing a protein or RNA, and human artificial chromosomes (see, e.g., Harrington et al., Nat Genet 15:345, 1997).
  • nonviral vectors useful for expression of the C5-binding polynucleotides and polypeptides in mammalian (e.g., human) cells include pThioHis A, B & C, pcDNA3.1/His, pEBVHis A, B & C, (Invitrogen, San Diego, Calif.), MPSV vectors, and numerous other vectors known in the art for expressing other proteins.
  • Useful viral vectors include vectors based on retroviruses, adenoviruses, adenoassociated viruses, herpes viruses, vectors based on SV40, papilloma virus, HBP Epstein Barr virus, vaccinia virus vectors and Semliki Forest virus (SFV). See, Brent et al., supra; Smith, Annu. Rev. Microbiol. 49:807, 1995; and Rosenfeld et al., Cell 68:143, 1992.
  • expression vector depends on the intended host cells in which the vector is to be expressed.
  • the expression vectors contain a promoter and other regulatory sequences (e.g., enhancers) that are operably linked to the polynucleotides encoding an C5-binding antibody chain or fragment.
  • an inducible promoter is employed to prevent expression of inserted sequences except under inducing conditions.
  • Inducible promoters include, e.g., arabinose, lacZ, metallothionein promoter or a heat shock promoter. Cultures of transformed organisms can be expanded under noninducing conditions without biasing the population for coding sequences whose expression products are better tolerated by the host cells.
  • promoters In addition to promoters, other regulatory elements may also be required or desired for efficient expression of an C5-binding antibody chain or fragment. These elements typically include an ATG initiation codon and adjacent ribosome binding site or other sequences. In addition, the efficiency of expression may be enhanced by the inclusion of enhancers appropriate to the cell system in use (see, e.g., Scharf et al., Results Probl. Cell Differ. 20:125, 1994; and Bittner et al., Meth. Enzymol., 153:516, 1987). For example, the SV40 enhancer or CMV enhancer may be used to increase expression in mammalian host cells.
  • the expression vectors may also provide a secretion signal sequence position to form a fusion protein with polypeptides encoded by inserted C5-binding antibody sequences. More often, the inserted C5-binding antibody sequences are linked to a signal sequences before inclusion in the vector.
  • Vectors to be used to receive sequences encoding C5-binding antibody light and heavy chain variable domains sometimes also encode constant regions or parts thereof. Such vectors allow expression of the variable regions as fusion proteins with the constant regions thereby leading to production of intact antibodies or fragments thereof. Typically, such constant regions are human.
  • the host cells for harboring and expressing the C5-binding antibody chains can be either prokaryotic or eukaryotic.
  • E. coli is one prokaryotic host useful for cloning and expressing the polynucleotides of the present invention.
  • Other microbial hosts suitable for use include bacilli, such as Bacillus subtilis , and other enterobacteriaceae, such as Salmonella, Serratia , and various Pseudomonas species.
  • bacilli such as Bacillus subtilis
  • enterobacteriaceae such as Salmonella, Serratia
  • various Pseudomonas species include bacilli, such as Bacillus subtilis , and other enterobacteriaceae, such as Salmonella, Serratia , and various Pseudomonas species.
  • expression vectors typically contain expression control sequences compatible with the host cell (e.g., an origin of replication).
  • any number of a variety of well-known promoters will be present, such as the lactose promoter system, a tryptophan (trp) promoter system, a beta-lactamase promoter system, or a promoter system from phage lambda.
  • the promoters typically control expression, optionally with an operator sequence, and have ribosome binding site sequences and the like, for initiating and completing transcription and translation.
  • Other microbes, such as yeast can also be employed to express C5-binding polypeptides of the invention. Insect cells in combination with baculovirus vectors can also be used.
  • mammalian host cells are used to express and produce the C5-binding polypeptides of the present invention.
  • they can be either a hybridoma cell line expressing endogenous immunoglobulin genes (e.g., the 1D6.C9 myeloma hybridoma clone as described in the Examples) or a mammalian cell line harboring an exogenous expression vector (e.g., the SP2/0 myeloma cells exemplified below).
  • endogenous immunoglobulin genes e.g., the 1D6.C9 myeloma hybridoma clone as described in the Examples
  • an exogenous expression vector e.g., the SP2/0 myeloma cells exemplified below.
  • a number of suitable host cell lines capable of secreting intact immunoglobulins have been developed including the CHO cell lines, various Cos cell lines, HeLa cells, myeloma cell lines, transformed B-cells and hybridomas.
  • the use of mammalian tissue cell culture to express polypeptides is discussed generally in, e.g., Winnacker, FROM GENES TO CLONES, VCH Publishers, N.Y., N.Y., 1987.
  • Expression vectors for mammalian host cells can include expression control sequences, such as an origin of replication, a promoter, and an enhancer (see, e.g., Queen, et al., Immunol. Rev.
  • expression vectors usually contain promoters derived from mammalian genes or from mammalian viruses. Suitable promoters may be constitutive, cell type-specific, stage-specific, and/or modulatable or regulatable.
  • Useful promoters include, but are not limited to, the metallothionein promoter, the constitutive adenovirus major late promoter, the dexamethasone-inducible MMTV promoter, the SV40 promoter, the MRP poIIII promoter, the constitutive MPSV promoter, the tetracycline-inducible CMV promoter (such as the human immediate-early CMV promoter), the constitutive CMV promoter, and promoter-enhancer combinations known in the art.
  • Methods for introducing expression vectors containing the polynucleotide sequences of interest vary depending on the type of cellular host. For example, calcium chloride transfection is commonly utilized for prokaryotic cells, whereas calcium phosphate treatment or electroporation may be used for other cellular hosts. (See generally Sambrook, et al., supra).
  • Other methods include, e.g., electroporation, calcium phosphate treatment, liposome-mediated transformation, injection and microinjection, ballistic methods, virosomes, immunoliposomes, polycation:nucleic acid conjugates, naked DNA, artificial virions, fusion to the herpes virus structural protein VP22 (Elliot and O'Hare, Cell 88:223, 1997), agent-enhanced uptake of DNA, and ex vivo transduction. For long-term, high-yield production of recombinant proteins, stable expression will often be desired.
  • cell lines which stably express C5-binding antibody chains or binding fragments can be prepared using expression vectors of the invention which contain viral origins of replication or endogenous expression elements and a selectable marker gene. Following the introduction of the vector, cells may be allowed to grow for 1-2 days in an enriched media before they are switched to selective media.
  • the purpose of the selectable marker is to confer resistance to selection, and its presence allows growth of cells which successfully express the introduced sequences in selective media.
  • Resistant, stably transfected cells can be proliferated using tissue culture techniques appropriate to the cell type.
  • Monoclonal antibodies can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein, 1975 Nature 256: 495. Many techniques for producing monoclonal antibody can be employed e.g., viral or oncogenic transformation of B lymphocytes.
  • hybridomas An animal system for preparing hybridomas is the murine system. Hybridoma production in the mouse is a well established procedure. Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.
  • Chimeric or humanized antibodies of the present invention can be prepared based on the sequence of a murine monoclonal antibody prepared as described above.
  • DNA encoding the heavy and light chain immunoglobulins can be obtained from the murine hybridoma of interest and engineered to contain non-murine (e.g., human) immunoglobulin sequences using standard molecular biology techniques.
  • the murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Pat. No. 4,816,567 to Cabilly et al.).
  • the murine CDR regions can be inserted into a human framework using methods known in the art. See e.g., U.S. Pat. No. 5,225,539 to Winter, and U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al.
  • the antibodies of the invention are human monoclonal antibodies.
  • Such human monoclonal antibodies directed against C5 can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system.
  • transgenic and transchromosomic mice include mice referred to herein as HuMAb mice and KM mice, respectively, and are collectively referred to herein as “human Ig mice.”
  • the HuMAb Mouse® (Medarex, Inc.) contains human immunoglobulin gene miniloci that encode un-rearranged human heavy ( ⁇ and ⁇ ) and ⁇ light chain immunoglobulin sequences, together with targeted mutations that inactivate the endogenous ⁇ and ⁇ chain loci (see e.g., Lonberg, et al., 1994 Nature 368(6474): 856-859). Accordingly, the mice exhibit reduced expression of mouse IgM or ⁇ , and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human IgG ⁇ monoclonal (Lonberg, N.
  • human antibodies of the invention can be raised using a mouse that carries human immunoglobulin sequences on transgenes and transchomosomes such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome.
  • KM mice a mouse that carries a human heavy chain transgene and a human light chain transchromosome.
  • transgenic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise C5-binding antibodies of the invention.
  • an alternative transgenic system referred to as the Xenomouse (Abgenix, Inc.) can be used.
  • Such mice are described in, e.g., U.S. Pat. Nos. 5,939,598; 6,075,181; 6,114,598; 6, 150,584 and 6,162,963 to Kucherlapati et al.
  • mice carrying both a human heavy chain transchromosome and a human light chain tranchromosome referred to as “TC mice” can be used; such mice are described in Tomizuka et al., 2000 Proc. Natl. Acad. Sci. USA 97:722-727.
  • cows carrying human heavy and light chain transchromosomes have been described in the art (Kuroiwa et al., 2002 Nature Biotechnology 20:889-894) and can be used to raise C5-binding antibodies of the invention.
  • Human monoclonal antibodies of the invention can also be prepared using phage display methods for screening libraries of human immunoglobulin genes. Such phage display methods for isolating human antibodies are established in the art or described in the examples below. See for example: U.S. Pat. Nos. 5,223,409; 5,403,484; and 5,571,698 to Ladner et al.; U.S. Pat. Nos. 5,427,908 and 5,580,717 to Dower et al.; U.S. Pat. Nos. 5,969,108 and 6,172,197 to McCafferty et al.; and U.S. Pat. Nos. 5,885,793; 6,521,404; 6,544,731; 6,555,313; 6,582,915 and 6,593,081 to Griffiths et al.
  • Human monoclonal antibodies of the invention can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization.
  • SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization.
  • Such mice are described in, for example, U.S. Pat. Nos. 5,476,996 and 5,698,767 to Wilson et al.
  • Engineered antibodies of the invention include those in which modifications have been made to framework residues within VH and/or VL, e.g. to improve the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody.
  • one approach is to “backmutate” one or more framework residues to the corresponding germline sequence. More specifically, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germline sequences from which the antibody is derived. To return the framework region sequences to their germline configuration, the somatic mutations can be “backmutated” to the germline sequence by, for example, site-directed mutagenesis. Such “backmutated” antibodies are also intended to be encompassed by the invention.
  • Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell-epitopes to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as “deimmunization” and is described in further detail in U.S. Patent Publication No. 20030153043 by Carr et al.
  • antibodies of the invention may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity.
  • an antibody of the invention may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody.
  • the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased.
  • This approach is described further in U.S. Pat. No. 5,677,425 by Bodmer et al.
  • the number of cysteine residues in the hinge region of CH1 is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
  • the Fc hinge region of an antibody is mutated to decrease the biological half-life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc-hinge fragment such that the antibody has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding.
  • SpA Staphylococcyl protein A
  • the antibody is modified to increase its biological half-life.
  • Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Pat. No. 6,277,375 to Ward.
  • the antibody can be altered within the CH1 or CL region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Pat. Nos. 5,869,046 and 6,121,022 by Presta et al.
  • the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector functions of the antibody.
  • one or more amino acids can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen-binding ability of the parent antibody.
  • the effector ligand to which affinity is altered can be, for example, an Fc receptor or the C1 component of complement. This approach is described in further detail in U.S. Pat. Nos. 5,624,821 and 5,648,260, both by Winter et al.
  • one or more amino acids selected from amino acid residues can be replaced with a different amino acid residue such that the antibody has altered C1q binding and/or reduced or abolished complement dependent cytotoxicity (CDC).
  • CDC complement dependent cytotoxicity
  • one or more amino acid residues are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
  • the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fc ⁇ receptor by modifying one or more amino acids.
  • ADCC antibody dependent cellular cytotoxicity
  • This approach is described further in PCT Publication WO 00/42072 by Presta.
  • the binding sites on human IgG1 for Fc ⁇ RI, Fc ⁇ RII, Fc ⁇ RIII and FcRn have been mapped and variants with improved binding have been described (see Shields, R. L. et al., 2001 J. Biol. Chen. 276:6591-6604).
  • the glycosylation of an antibody is modified.
  • an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation).
  • Glycosylation can be altered to, for example, increase the affinity of the antibody for “antigen’.
  • Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence.
  • one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site.
  • Such aglycosylation may increase the affinity of the antibody for antigen.
  • an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures.
  • altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies.
  • carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation.
  • glycoprotein-modifying glycosyl transferases e.g., beta(1,4)-N acetylglucosaminyltransferase III (GnTIII)
  • GnTIII glycoprotein-modifying glycosyl transferases
  • the C5-binding antibodies having VH and VL sequences or full length heavy and light chain sequences shown herein can be used to create new C5-binding antibodies by modifying full length heavy chain and/or light chain sequences, VH and/or VL sequences, or the constant region(s) attached thereto.
  • the structural features of a C5-binding antibody of the invention are used to create structurally related C5-binding antibodies that retain at least one functional property of the antibodies of the invention, such as binding to human C5 and also inhibiting one or more functional properties of C5 (e.g., inhibit red blood cell lysis in a hemolytic assay).
  • one or more CDR regions of the antibodies of the present invention, or mutations thereof can be combined recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, C5-binding antibodies of the invention, as discussed above.
  • the starting material for the engineering method is one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof.
  • To create the engineered antibody it is not necessary to actually prepare (i.e., express as a protein) an antibody having one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. Rather, the information contained in the sequence(s) is used as the starting material to create a “second generation” sequence(s) derived from the original sequence(s) and then the “second generation” sequence(s) is prepared and expressed as a protein.
  • the invention provides a method for preparing an C5-binding antibody consisting of: a heavy chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237; and a light chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238, a CDR2 sequence selected from the group consisting of SEQ ID
  • the invention provides a method for preparing an C5-binding antibody optimized for expression in a mammalian cell consisting of: a full length heavy chain antibody sequence having a sequence selected from the group of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282; and a full length light chain antibody sequence having a sequence selected from the group of 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290; altering at least one amino acid residue within the full length heavy chain antibody sequence and/or the full length light chain antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.
  • the altered antibody sequence can also be prepared by screening antibody libraries having fixed CDR3 sequences or minimal essential binding determinants as described in US20050255552 and diversity on CDR1 and CDR2 sequences.
  • the screening can be performed according to any screening technology appropriate for screening antibodies from antibody libraries, such as phage display technology.
  • Standard molecular biology techniques can be used to prepare and express the altered antibody sequence.
  • the antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the functional properties of the C5-binding antibodies described herein, which functional properties include, but are not limited to, specifically binding to human and/or cynomolgus C5; and the antibody inhibit red blood cell lysis in a hemolytic assay.
  • the functional properties of the altered antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g., ELISAs).
  • mutations can be introduced randomly or selectively along all or part of an C5-binding antibody coding sequence and the resulting modified C5-binding antibodies can be screened for binding activity and/or other functional properties as described herein.
  • Mutational methods have been described in the art.
  • PCT Publication WO 02/092780 by Short describes methods for creating and screening antibody mutations using saturation mutagenesis, synthetic ligation assembly, or a combination thereof.
  • PCT Publication WO 03/074679 by Lazar et al. describes methods of using computational screening methods to optimize physiochemical properties of antibodies.
  • the antibodies of the invention can be characterized by various functional assays. For example, they can be characterized by their ability to inhibit red blood cell lysis in hemolytic assays, their affinity to a C5 protein (e.g., human and/or cynomolgus C5), the epitope binning, their resistance to proteolysis, and their ability to block the complement cascade, for example, their ability to inhibit MAC formation.
  • C5 protein e.g., human and/or cynomolgus C5
  • the epitope binning e.g., human and/or cynomolgus C5
  • the epitope binning e.g., human and/or cynomolgus C5
  • the epitope binning e.g., human and/or cynomolgus C5
  • the epitope binning e.g., human and/or cynomolgus C5
  • complement activity can be monitored by (i) measurement of inhibition of complement-mediated lysis of red blood cells (hemolysis); (ii) measurement of ability to inhibit cleavage of C3 or C5; and (iii) inhibition of alternative pathway mediated hemolysis.
  • hemolytic assays see, e.g., Baatrup et al., Ann Rheum Dis, 51:892-7, 1992
  • immunological assays see, e.g., Auda et al., Rheumatol Int, 10:185-9, 1990.
  • the hemolytic techniques measure the functional capacity of the entire sequence-either the classical or alternative pathway.
  • Immunological techniques measure the protein concentration of a specific complement component or split product.
  • T cell proliferation assay Chain et al., J Immunol Methods, 99:221-8, 1987
  • DTH delayed type hypersensitivity
  • hemolytic techniques In hemolytic techniques, all of the complement components must be present and functional. Therefore hemolytic techniques can screen both functional integrity and deficiencies of the complement system (see, e.g., Dijk et al., J Immunol Methods 36: 29-39, 1980; Minh et al., Clin Lab Haematol. 5:23-34 1983; and Tanaka et al., J Immunol 86: 161-170, 1986).
  • sheep red blood cells coated with hemolysin (rabbit IgG to sheep red blood cells) or chicken red blood cells that are sensitized with rabbit anti-chicken antibodies are used as target cells (sensitized cells).
  • MAC deposition assay can be performed. Briefly, zymosan can be used to activate the alternative pathway and IgM can be used to active the classic pathway. Fabs are pre-inclubated with human serum and added to plates coated with zymosan or IgM. Percentage inhibition of MAC deposition can be calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum).
  • the ability of an antibody to bind to C5 can be detected by labelling the antibody of interest directly, or the antibody may be unlabelled and binding detected indirectly using various sandwich assay formats known in the art.
  • the C5-binding antibodies of the invention block or compete with binding of a reference C5-binding antibody to a C5 polypeptide.
  • a reference C5-binding antibody can be fully human C5-binding antibodies described above. They can also be other mouse, chimeric or humanized C5-binding antibodies which bind to the same epitope as the reference antibody.
  • the capacity to block or compete with the reference antibody binding indicates that a C5-binding antibody under test binds to the same or similar epitope as that defined by the reference antibody, or to an epitope which is sufficiently proximal to the epitope bound by the reference C5-binding antibody.
  • Such antibodies are especially likely to share the advantageous properties identified for the reference antibody.
  • the capacity to block or compete with the reference antibody may be determined by, e.g., a competition binding assay.
  • a competition binding assay the antibody under test is examined for ability to inhibit specific binding of the reference antibody to a common antigen, such as a C5 polypeptide.
  • a test antibody competes with the reference antibody for specific binding to the antigen if an excess of the test antibody substantially inhibits binding of the reference antibody.
  • Substantial inhibition means that the test antibody reduces specific binding of the reference antibody usually by at least 10%, 25%, 50%, 75%, or 90%.
  • solid phase direct labeled assay solid phase direct labeled sandwich assay (see Harlow & Lane, supra); solid phase direct label RIA using 1-125 label (see Morel et al., Molec. Immunol. 25:7-15, 1988); solid phase direct biotin-avidin EIA (Cheung et al., Virology 176:546-552, 1990); and direct labeled RIA (Moldenhauer et al., Scand. J. Immunol. 32:77-82, 1990).
  • an assay involves the use of purified antigen bound to a solid surface or cells bearing either of these, an unlabelled test C5-binding antibody and a labelled reference antibody.
  • Antibodies identified by competition assay include antibodies binding to the same epitope as the reference antibody and antibodies binding to an adjacent epitope sufficiently proximal to the epitope bound by the reference antibody for steric hindrance to occur.
  • each antibody can be biotinylated using commercially available reagents (e.g., reagents from Pierce, Rockford, Ill.). Competition studies using unlabeled monoclonal antibodies and biotinylated monoclonal antibodies can be performed using a C5 polypeptide coated-ELISA plates. Biotinylated MAb binding can be detected with a strep-avidin-alkaline phosphatase probe. To determine the isotype of a purified C5-binding antibody, isotype ELISAs can be performed.
  • wells of microtiter plates can be coated with 1 ⁇ g/ml of anti-human IgG overnight at 4° C. After blocking with 1% BSA, the plates are reacted with 1 ⁇ g/ml or less of the monoclonal C5-binding antibody or purified isotype controls, at ambient temperature for one to two hours. The wells can then be reacted with either human IgGI or human IgM-specific alkaline phosphatase-conjugated probes. Plates are then developed and analyzed so that the isotype of the purified antibody can be determined.
  • flow cytometry can be used. Briefly, cell lines expressing C5 (grown under standard growth conditions) can be mixed with various concentrations of a C5-binding antibody in PBS containing 0.1% BSA and 10% fetal calf serum, and incubated at 37° C. for 1 hour. After washing, the cells are reacted with Fluorescein-labeled anti-human IgG antibody under the same conditions as the primary antibody staining. The samples can be analyzed by FACScan instrument using light and side scatter properties to gate on single cells. An alternative assay using fluorescence microscopy may be used (in addition to or instead of) the flow cytometry assay. Cells can be stained exactly as described above and examined by fluorescence microscopy. This method allows visualization of individual cells, but may have diminished sensitivity depending on the density of the antigen.
  • C5-binding antibodies of the invention can be further tested for reactivity with a C5 polypeptide or antigenic fragment by Western blotting. Briefly, purified C5 polypeptides or fusion proteins, or cell extracts from cells expressing C5 can be prepared and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. After electrophoresis, the separated antigens are transferred to nitrocellulose membranes, blocked with 10% fetal calf serum, and probed with the monoclonal antibodies to be tested. Human IgG binding can be detected using anti-human IgG alkaline phosphatase and developed with BCIP/NBT substrate tablets (Sigma Chem. Co., St. Louis, Mo.).
  • the present invention provides methods of treating a disease or disorder associated with increased complement activity by administering to a subject in need thereof an effective amount of the antibodies of the invention.
  • the present invention provides a method of treating age-related macular degeneration (AMD) by administering to a subject in need thereof an effective amount of the antibodies of the invention.
  • AMD age-related macular degeneration
  • the antibodies of the invention can be used, inter alia, to prevent progression of dry AMD to wet AMD, to slow and/or prevent progression of geographic atrophy, and to improve vision lost due to dry AMD progression. It can also be used in combination with anti-VEGF therapies for the treatment of wet AMD patients.
  • the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention.
  • complement related diseases or disorders include: neurological disorders, multiple sclerosis, stroke, Guillain Barre Syndrome, traumatic brain injury, Parkinson's disease, disorders of inappropriate or undesirable complement activation, hemodialysis complications, hyperacute allograft rejection, xenograft rejection, interleukin-2 induced toxicity during IL-2 therapy, inflammatory disorders, inflammation of autoimmune diseases, Crohn's disease, adult respiratory distress syndrome, thermal injury including burns or frostbite, post-ischemic reperfusion conditions, myocardial infarction, balloon angioplasty, post-pump syndrome in cardiopulmonary bypass or renal bypass, hemodialysis, renal ischemia, mesenteric artery reperfusion after acrotic reconstruction, infectious disease or sepsis, immune complex disorders and autoimmune diseases, rheumatoid arthritis, systemic lupus erythemato
  • lung disease and disorders such as dyspnea, hemoptysis, ARDS, asthma, chronic obstructive pulmonary disease (COPD), emphysema, pulmonary embolisms and infarcts, pneumonia, fibrogenic dust diseases, inert dusts and minerals (e.g., silicon, coal dust, beryllium, and asbestos), pulmonary fibrosis, organic dust diseases, chemical injury (due to irritant gasses and chemicals, e.g., chlorine, phosgene, sulfur dioxide, hydrogen sulfide, nitrogen dioxide, ammonia, and hydrochloric acid), smoke injury, thermal injury (e.g., burn, freeze), asthma, allergy, bronchoconstriction, hypersensitivity pneumonitis, parasitic diseases, Goodpasture's Syndrome, pulmonary vasculitis, and immune complex-associated inflammation.
  • COPD chronic obstructive pulmonary disease
  • emphysema pulmonary embolisms and infarcts
  • pneumonia fibrogenic
  • the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention, wherein said disease or disorder is asthma, arthritis (e.g., rheumatoid arthritis), autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer-Simons Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, diseases of the central nervous system such as Alzheimer's disease and other neurodegenerative conditions, aHUS, glomerulonephritis, bullous pemphigoid or MPGN II.
  • asthma arthritis
  • arthritis e.g., rheumatoid arthritis
  • autoimmune heart disease multiple sclerosis
  • inflammatory bowel disease ischemia-reperfusion injuries
  • Barraquer-Simons Syndrome hemodialysis
  • systemic lupus
  • the present invention provides methods of treating glomerulonephritis by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention.
  • Symptoms of glomerulonephritis include, but not limited to, proteinuria; reduced glomerular filtration rate (GFR); serum electrolyte changes including azotemia (uremia, excessive blood urea nitrogen—BUN) and salt retention, leading to water retention resulting in hypertension and edema; hematuria and abnormal urinary sediments including red cell casts; hypoalbuminemia; hyperlipidemia; and lipiduria.
  • the present invention provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention.
  • PNH paroxysmal nocturnal hemoglobinuria
  • the present invention provides methods of reducing the dysfunction of the immune and hemostatic systems associated with extracorporeal circulation by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention.
  • the antibodies of the present invention can be used in any procedure which involves circulating the patient's blood from a blood vessel of the patient, through a conduit, and back to a blood vessel of the patient, the conduit having a luminal surface comprising a material capable of causing at least one of complement activation, platelet activation, leukocyte activation, or platelet-leukocyte adhesion.
  • Such procedures include, but are not limited to, all forms of ECC, as well as procedures involving the introduction of an artificial or foreign organ, tissue, or vessel into the blood circuit of a patient.
  • Subjects to be treated with therapeutic agents of the present invention can also be administered other therapeutic agents with know methods of treating conditions associated with macular degeneration, such as antibiotic treatments as described in U.S. Pat. No. 6,218,368.
  • immunosuppressive agents such as cyclosporine
  • cytotoxic drugs corticosteriods, nonsteroidal anti-inflammatory drugs (NSAIDs), specific T-lymphocyte immunosuppressants, and antibodies or fragments thereof (see Physicians' Desk Reference, 53rd edition, Medical Economics Company Inc., Montvale, N.J. (1999).
  • Immunosuppressive treatment is typically continued at intervals for a period of a week, a month, three months, six months or a year. In some patients, treatment is administered for up to the rest of a patient's life.
  • an antibody of the present invention is administered to a subject who is also receiving therapy with a second agent (e.g., verteporfin).
  • a second agent e.g., verteporfin
  • the binding molecule is administered in conjunction with surgical treatments.
  • Suitable agents for combination treatment with C5-binding antibodies include agents known in the art that are able to modulate the activities of complement components (see, e.g., U.S. Pat. No. 5,808,109). Other agents have been reported to diminish complement-mediated activity. Such agents include: amino acids (Takada, Y. et al. Immunology 1978, 34, 509); phosphonate esters (Becker, L. Biochem. Biophy. Acta 1967, 147, 289); polyanionic substances (Conrow, R. B. et al. J. Med. Chem. 1980, 23, 242); sulfonyl fluorides (Hansch, C.; Yoshimoto, M. J. Med. Chem.
  • K-76 a fungal metabolite from Stachybotrys
  • Both K-76 and K-76 COOH have been shown to inhibit complement mainly at the C5 step (Hong et al., J. Immunol. 122: 2418, 1979; Miyazaki et al., Microbiol. Immunol. 24: 1091, 1980), and to prevent the generation of a chemotactic factor from normal human complement (Bumpers et al., Lab. Clinc. Med. 102: 421, 1983).
  • K-76 or K-76 COOH has also been reported to inhibit the C3b inactivator system of complement (Hong et al., J. Immunol. 127: 104-108, 1981).
  • Other suitable agents for practicing methods of the present invention include griseofulvin (Weinberg, in Principles of Medicinal Chemistry, 2d Ed., Foye, W. O., ed., Lea & Febiger, Philadelphia, Pa., p. 813, 1981), isopannarin (Djura et al., Aust. J. Chem. 36: 1057, 1983), and metabolites of Siphonodictyon coralli-phagum (Sullivan et al., Tetrahedron 37: 979, 1981).
  • a combination therapy regimen may be additive, or it may produce synergistic results (e.g., reductions in complement pathway activity more than expected for the combined use of the two agents).
  • the present invention provide a combination therapy for preventing and/or treating AMD or another complement related disease as described above with a C5-binding antibody of the invention and an anti-angiogenic, such as anti-VEGF agent.
  • the invention encompasses diagnostic assays for determining C5 protein and/or nucleic acid expression as well as C5 protein function, in the context of a biological sample (e.g., blood, serum, cells, tissue) or from individual is afflicted with a disease or disorder, or is at risk of developing a disorder associated with AMD.
  • a biological sample e.g., blood, serum, cells, tissue
  • Diagnostic assays such as competitive assays rely on the ability of a labelled analogue (the “tracer”) to compete with the test sample analyte for a limited number of binding sites on a common binding partner.
  • the binding partner generally is insolubilized before or after the competition and then the tracer and analyte bound to the binding partner are separated from the unbound tracer and analyte. This separation is accomplished by decanting (where the binding partner was preinsolubilized) or by centrifuging (where the binding partner was precipitated after the competitive reaction).
  • the amount of test sample analyte is inversely proportional to the amount of bound tracer as measured by the amount of marker substance.
  • Dose-response curves with known amounts of analyte are prepared and compared with the test results in order to quantitatively determine the amount of analyte present in the test sample.
  • These assays are called ELISA systems when enzymes are used as the detectable markers.
  • competitive binding between antibodies and C5-binding antibodies results in the bound C5 protein, preferably the C5 epitopes of the invention, being a measure of antibodies in the serum sample, most particularly, neutralising antibodies in the serum sample.
  • a significant advantage of the assay is that measurement is made of neutralising antibodies directly (i.e., those which interfere with binding of C5 protein, specifically, epitopes).
  • Such an assay particularly in the form of an ELISA test has considerable applications in the clinical environment and in routine blood screening.
  • Immunologic techniques employ polyclonal or monoclonal antibodies against the different epitopes of the various complement components (e.g., C3, C4, C5) to detect, e.g., the split products of complement components (see, e.g., Hugli et al., Immunoassays Clinical Laboratory Techniques 443-460, 1980; Gorski et al., J Immunol Meth 47: 61-73, 1981; Linder et al., J Immunol Meth 47: 49-59, 1981; and Burger et al., J Immunol 141: 553-558, 1988). Binding of the antibody with the split product in competition with a known concentration of labeled split product could then be measured.
  • Various assays such as radio-immunoassays, ELISA's, and radial diffusion assays are available to detect complement split products.
  • the immunologic techniques provide high sensitivity to detect complement activation, since they allow measurement of split-product formation in blood from a test subject and control subjects with or without macular degeneration-related disorders. Accordingly, in some methods of the present invention, diagnosis of a disorder associated with ocular disorders is obtained by measurement of abnormal complement activation through quantification of the soluble split products of complement components in blood plasma from a test subject.
  • the measurements can be performed as described, e.g., in Chenoweth et al., N Engl J Med 304: 497-502, 1981; and Bhakdi et al., Biochim Biophys Acta 737: 343-372, 1983.
  • Preferably, only the complement activation formed in vivo is measured. This can be accomplished by collecting a biological sample from the subject (e.g., serum) in medium containing inhibitors of the complement system, and subsequently measuring complement activation (e.g., quantification of the split products) in the sample.
  • the detection of complement proteins in comparison to the levels in a corresponding biological sample from a normal subject is indicative of a patient with disorders associated with macular degeneration.
  • In vivo diagnostic or imaging is described in US2006/0067935. Briefly, these methods generally comprise administering or introducing to a patient a diagnostically effective amount of a C5 binding molecule that is operatively attached to a marker or label that is detectable by non-invasive methods.
  • the antibody-marker conjugate is allowed sufficient time to localize and bind to complement proteins within the eye.
  • the patient is then exposed to a detection device to identify the detectable marker, thus forming an image of the location of the C5 binding molecules in the eye of a patient.
  • the presence of C5 binding antibody or an antigen-binding fragment thereof is detected by determining whether an antibody-marker binds to a component of the eye.
  • Detection of an increased level in selected complement proteins or a combination of protein in comparison to a normal individual without AMD disease is indicative of a predisposition for and/or on set of disorders associated with macular degeneration.
  • These aspects of the invention are also preferred for use in eye imaging methods and combined angiogenic diagnostic and treatment methods.
  • the invention also pertains to the field of predictive medicine in which diagnostic assays, prognostic assays, pharmacogenomics, and monitoring clinical trials are used for prognostic (predictive) purposes to thereby treat an individual prophylactically.
  • the invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with dysregulation of complement pathway activity. For example, mutations in a C5 gene can be assayed in a biological sample. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with C5 protein, nucleic acid expression or activity.
  • Another aspect of the invention provides methods for determining C5 nucleic acid expression or C5 protein activity in an individual to thereby select appropriate therapeutic or prophylactic agents for that individual (referred to herein as “pharmacogenomics”).
  • Pharmacogenomics allows for the selection of agents (e.g., drugs) for therapeutic or prophylactic treatment of an individual based on the genotype of the individual (e.g., the genotype of the individual examined to determine the ability of the individual to respond to a particular agent.)
  • Yet another aspect of the invention pertains to monitoring the influence of agents (e.g., drugs) on the expression or activity of C5 protein in clinical trials.
  • agents e.g., drugs
  • compositions comprising the C5-binding antibodies (intact or binding fragments) formulated together with a pharmaceutically acceptable carrier.
  • the compositions can additionally contain one or more other therapeutica agents that are suitable for treating or preventing a complement-associated disease (e.g., AMD).
  • Pharmaceutically carriers enhance or stabilize the composition, or to facilitate preparation of the composition.
  • Pharmaceutically acceptable carriers include solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.
  • a pharmaceutical composition of the present invention can be administered by a variety of methods known in the art.
  • the route and/or mode of administration vary depending upon the desired results. It is preferred that administration be intravenous, intramuscular, intraperitoneal, or subcutaneous, or administered proximal to the site of the target.
  • the antibodies of the invention are formulated so that they can be administered intravitreally into the eye.
  • the pharmaceutically acceptable carrier should be suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion).
  • the active compound i.e., antibody, bispecific and multispecific molecule, may be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound.
  • the composition should be sterile and fluid. Proper fluidity can be maintained, for example, by use of coating such as lecithin, by maintenance of required particle size in the case of dispersion and by use of surfactants. In many cases, it is preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol or sorbitol, and sodium chloride in the composition. Long-term absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.
  • compositions of the invention can be prepared in accordance with methods well known and routinely practiced in the art. See, e.g., Remington: The Science and Practice of Pharmacy, Mack Publishing Co., 20th ed., 2000; and Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, ed., Marcel Dekker, Inc., New York, 1978. Pharmaceutical compositions are preferably manufactured under GMP conditions. Typically, a therapeutically effective dose or efficacious dose of the C5-binding antibody is employed in the pharmaceutical compositions of the invention.
  • the C5-binding antibodies are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art. Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response).
  • Dosage unit form refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present invention can be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient.
  • the selected dosage level depends upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors.
  • a physician or veterinarian can start doses of the antibodies of the invention employed in the pharmaceutical composition at levels lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved.
  • effective doses of the compositions of the present invention, for the treatment of an allergic inflammatory disorder described herein vary depending upon many different factors, including means of administration, target site, physiological state of the patient, whether the patient is human or an animal, other medications administered, and whether treatment is prophylactic or therapeutic. Treatment dosages need to be titrated to optimize safety and efficacy.
  • the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 15 mg/kg, of the host body weight.
  • An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months.
  • the dosage ranges from about 0.0001 to about 10 mg.
  • An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months.
  • Antibody is usually administered on multiple occasions. Intervals between single dosages can be weekly, monthly or yearly. Intervals can also be irregular as indicated by measuring blood levels of C5-binding antibody in the patient. In some methods of systemic administration, dosage is adjusted to achieve a plasma antibody concentration of 1-1000 ⁇ g/ml and in some methods 25-500 ⁇ g/ml. Alternatively, antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, humanized antibodies show longer half life than that of chimeric antibodies and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic.
  • a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives.
  • a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime.
  • Cynomolgus C5 was purified successfully from cynomolgus serum by affinity chromatography using MOR07086 hu IgG1. Cynomolgus C5 was quality tested by SDS-PAGE, Western blot, mass spectrometry and hemolytic assays. Quality of purified cynomolgus C5 was shown to be high by SDS-PAGE and Western blot. Lack of C3 contamination was confirmed by SDS and Western blot. In addition, the identity of cynomolgus C5 sequence was determined by mass spectrometric analysis and the activity of purified cynomolgus C5 was tested in hemolytic assays. In hemolytic assays the new preparation was equipotent to human C5 (e.g., Sample 6, which was used in affinity maturation pannings, reconstituted complement activity of 20% human C5-depleted serum with similar activity to purified human C5).
  • human C5 e.g., Sample 6, which was used in affinity maturation
  • Bioactivity of purified human C5 was characterized and confirmed by the alternative pathway hemolytic activity.
  • C5 was spiked into C5-depleted human serum at varying concentrations to obtain an EC50.
  • EC50 values ranging between 0.02-0.1 nM were considered acceptable.
  • bioactivity of every purified human C5 protein lot was tested in the hemolytic assay.
  • the same quality control was done for cynomolgus C5 after purification from cynomolgus serum.
  • bioactivity of the material was also tested in hemolytic assays, in order to analyze if there was a loss of activity caused by biotinylation.
  • C5 antibodies were generated by selection of clones having high binding affinities, using as the source of antibody variant proteins a commercially available phage display library, the MorphoSys HuCAL GOLD® library.
  • HuCAL GOLD® library is a Fab library (Knappik et al., 2000) in which all six CDRs are diversified by appropriate mutation, and which employs the CysDisplayTM technology for linking the Fab to the phage surface (WO01/05950, Löhning et al., 2001).
  • the HuCAL GOLD® library was amplified in 2 ⁇ YT medium containing 34 ⁇ g/ml chloramphenicol and 1% glucose (2 ⁇ YT-CG). After infection with VCSM13 helper phage at an OD600 nm of 0.5 (30 min at 37° C. without shaking; 30 min at 37° C. shaking at 250 rpm), cells were spun down (4120 g; 5 min; 4° C.), resuspended in 2 ⁇ YT/34 ⁇ g/ml chloramphenicol/50 ⁇ g/ml kanamycin/0.25 mM IPTG and grown overnight at 22° C.
  • Phage were PEG-precipitated from the supernatant, resuspended in PBS/20% glycerol and stored at ⁇ 80° C. Phage amplification between two panning rounds was conducted as follows: mid-log phase E. coli TG1 cells were infected with eluted phage and plated onto LB-agar supplemented with 1% of glucose and 34 ⁇ g/ml of chloramphenicol (LB-CG plates). After overnight incubation at 30° C., the TG1 colonies were scraped off the agar plates and used to inoculate 2 ⁇ YT-CG until an OD600 nm of 0.5 was reached. VCSM13 helper phage were added for infection as described above.
  • the first panning variant was solid phase panning alternating human C5 (first and third round of selection) and cynomolgus C5 (second round of selection).
  • Three wells of a Maxisorp plate (F96 Nunc-Immunoplate) were coated with 200 ⁇ l of 50 nM C5 each o/n at 4° C.
  • the coated wells were washed 2 ⁇ with 400 ⁇ l PBS and blocked with 350 ⁇ l 5% MPBS for 2 h at RT on a microtiter plate shaker.
  • For each panning about 10 13 HuCAL GOLD® phage-antibodies were blocked with equal volume of PBST/5% milk powder for 2 h at room temperature.
  • the coated wells were washed 2 ⁇ with 400 ⁇ l PBS after the blocking procedure.
  • Elution of phage from the plate was performed with 200 ⁇ l 20 mM DTT in 10 mM Tris/HCl pH8 per well for 10 min.
  • the DTT phage eluate was added to 15 ml of E. coli TG1, which were grown to an OD600 of 0.6-0.8 at 37° C. in 2YT medium and incubated in 50 ml plastic tubes for 45 min at 37° C. without shaking for phage infection.
  • the bacterial pellets were each resuspended in 600 ⁇ l 2 ⁇ YT medium, plated on 3 ⁇ YT-CG agar plates and incubated overnight at 37° C. Colonies were scraped off the plates and phages were rescued and amplified as described above.
  • the second and third rounds of solid phase panning were performed according to the protocol of the first round.
  • the output of the first round was used for selections on cynomolgus C5 in order to enrich for cynomolgus cross-reactive antibodies.
  • washing stringency was increased and antigen concentration was decreased within the three round of selection in order to generate high affinity antibodies.
  • the HuCAL GOLD® phagemid library was used to select specific Fab antibody fragments against human C5.
  • First strategy was a solid phase panning on directly coated human C5 protein (panning procedure described above).
  • the enriched phage pools were subcloned from the pMORPH®23 library vector (allowing efficient antibody display on the phage surface) into the pMORPH® ⁇ 9_Fab_MH expression vector which mediates periplasmic expression of soluble Fabs. Single clones were picked and soluble Fabs were expressed from these single clones.
  • the 1st round output of the most successful 12 human solid phase pannings was used for selections on cynomolgus C5 (protein batch contaminated with cynomolgus C3; not known during pannings). 376 clones were confirmed in a secondary screening for binding to human C5 and 361 clones for binding to cynomolgus C5 captured from cynomolgus serum.
  • the second panning variant was solution panning against biologically active (in hemolytic assays) biotinylated human C5 and biotinylated cynomolgus C5.
  • Beads were then washed 7 ⁇ in PBS/0.05% Tween using a rotator, followed by washing another three times with PBS. Elution of phage from the Dynabeads was performed by adding 200 ⁇ l 20 mM DTT in 10 mM Tris/HCl pH 8 to each tube and incubation for 10 min. Dynabeads were removed by the magnetic particle separator and the supernatant was added to 15 ml of an E. coli TG-1 culture grown to OD600 nm of 0.6-0.8. Beads were then washed once with 200 ⁇ l PBS and together with additionally removed phages the PBS was added to the 15 ml E. coli TG-1 culture.
  • the culture was incubated in 50 ml plastic tubes for 45 min at 37° C. without shaking. After centrifugation for 5 min at 4120 ⁇ g, the bacterial pellets were resuspended each in 600 ⁇ l 2 ⁇ YT medium, plated on 3 ⁇ YT-CG agar plates and incubated overnight at 37° C. Colonies were scraped off the plates and phages were rescued and amplified as described above. The second and third rounds of selection were performed in an identical way to the first round of selection.
  • a further panning strategy was solution panning using human C5 and alternating human and cynomolgus C5 (protein batch contaminated with cynomolgus C3, not known during pannings). Therefore the proteins were biotinylated and the retained bio-functionality after the biotinylation procedure was confirmed in hemolytic bioassays.
  • the phage-antigen complex was captured on Streptavidin magnetic beads via the biotin moiety of the antigen. After washing only specific bound phage were eluted (panning procedure described above).
  • the Fab-encoding inserts of the selected HuCAL GOLD® phages were subcloned via XbaI and EcoRI into the E. coli expression vector pMORPH®x9_MH.
  • Fab fragments carry a C-terminal Myc tag and as a second C-terminal tag the 6 ⁇ His-tag (Chen et al., Gene 139:73-75 (1994)). After transformation of the expression plasmids into E.
  • Fab fragments encoded by pMORPH® x9_Fab_MH in TG-1 cells was carried out in shaker flask cultures using 750 ml of 2 ⁇ YT medium supplemented with 34 ⁇ g/ml chloramphenicol. Cultures were shaken at 30° C. until the OD600 nm reached 0.5. Expression was induced by addition of 0.75 mM IPTG for 20 h at 30° C. Cells were disrupted using lysozyme and Fab fragments isolated by Ni-NTA chromatography (Qiagen, Hilden, Germany). Buffer exchange to 1 ⁇ Dulbecco's PBS (pH 7.2) was performed using PD10 columns.
  • Samples were filtered sterile (0.2 ⁇ m, Millipore). Purity of samples was determined in denatured, reduced state by SDS-PAGE (15% Criterion Gels, BioRad) and in native state by size exclusion chromatography (HP-SEC). Protein concentrations were determined by UV-spectrophotometry (Krebs et al., J. Immunol. Methods 254:67-84 (2001)).
  • Enzyme Linked Immunosorbent Assay methods describe the screening of C5-binding antibodies (as bacterial BEL lysates or purified Fabs) on specific and counter antigens.
  • PBST PBS/0.05% Tween 20
  • BEL-extracts, purified HuCAL GOLD® Fabs or control Fabs diluted in PBS were added and incubated for 1 h at RT.
  • anti-HIS6 antibody coupled to peroxidase was applied (Roche).
  • fluorogenic substrate QuantaBlu (Pierce) was used according to manufacturer's instructions. Between all incubation steps, the wells of the microtiter plates were washed three times and five times with PBST after the final incubation with the secondary antibody. Fluorescence was measured in a Tecan GENios Pro plate reader.
  • the ELISA method described below was used for screening of HuCAL GOLD® Fabs after solution pannings using biotinylated complement proteins.
  • NeutrAvidin plates were blocked with 1 ⁇ Chemiblocker (Chemicon) diluted in PBS o/n at 4° C. These plates were used to screen for binding to human C5 and to the counter targets C3 and C4.
  • Maxisorp 384 well plates (Nunc, Rochester, N.Y., USA) were coated with 20 ⁇ l per well of 5 ⁇ g/ml sheep anti-human IgG, Fd fragment specific (The Binding Site, Birmingham, UK), diluted in PBS, pH 7.4. These plates were used to check for Fab expression levels and for non-specific biotin binding.
  • biotinylated human C5 to detect specific binding
  • biotinylated human C3 and C4 to detect unwanted binding
  • the biotinylated antigens were incubated with the HuCAL GOLD® Fabs for 1-2 h at RT.
  • Biotinylated unrelated antigen Transferrin was then added to the Maxisorp plates to check for biotin binding Fabs (in this case the HuCAL®-Fab fragments were previously captured via anti-Fd antibody).
  • fluorogenic substrate AttoPhos (Roche Diagnostics, Mannheim, Germany) and for detection of POD-conjugates, fluorogenic substrate QuantaBlu (Pierce) were used according to manufacturer's instructions. Fluorescence was measured in a Tecan GENios Pro plate reader.
  • a polyclonal C5-binding antibody (US Biological Cat#C7850-24) was used to capture cynomolgus C5 from cynomolgus serum.
  • L-CDR3 and H-CDR2 regions were optimized in parallel by cassette mutagenesis using trinucleotide directed mutagenesis (see e.g., Virnekas et al., Nucleic Acids Res. 22:5600-5607 (1994)), while the framework regions were kept constant.
  • cassette mutagenesis using trinucleotide directed mutagenesis (see e.g., Virnekas et al., Nucleic Acids Res. 22:5600-5607 (1994)
  • the framework regions were kept constant.
  • all parental Fab fragments were transferred from the corresponding expression vector (pMORPH®x9_MH) into the CysDisplayTM vector pMORPH®25 via XbaI/EcoRI.
  • pMORPH®25 was created from the HuCAL GOLD® display vector pMORPH®23 by removal of one BssHII site interfering with library cloning for H-CDR2 optimization.
  • L-CDR3 of parental Fabs the L-CDR3, framework 4 and the constant region of the light chains (405 bp) of the binders were removed by Bpil/Sphl and replaced by a repertoire of diversified L-CDR3s together with framework 4 and the constant domain.
  • the H-CDR2 (Xhol/BssHII) was diversified while the connecting framework regions were kept constant.
  • the parental H-CDR2 was replaced by a dummy before the diversified H-CDR2 cassette was cloned in.
  • the HuCAL® maturation libraries were amplified in 2 ⁇ YT medium containing 34 ⁇ g/ml chloramphenicol and 1% glucose (2 ⁇ YT-CG). After infection with VCSM13 helper phage at an OD600 nm of 0.5 (30 min at 37° C. without shaking; 30 min at 37° C. shaking at 250 rpm), cells were spun down (4120 ⁇ g; 5 min; 4° C.), resuspended in 2 ⁇ YT/34 ⁇ g/ml chloramphenicol/50 ⁇ g/ml kanamycin/0.25 mM IPTG and grown o/n at 22° C. Phages were PEG-precipitated twice from the supernatant, resuspended in PBS and used for the maturation pannings described below.
  • Solution pannings using the respective phage pools were either performed using biotinylated human C5 or alternating biotinylated human and cynomolgus C5 proteins.
  • antigen concentration was decreased and prolonged washing periods were applied (washing conditions are listed in Table 3).
  • Pre-blocked phage (1:2 mixture with 2 ⁇ Chemiblocker incubated for 1 h at RT) were incubated with low concentration of biotinylated C5 protein for 1-2 h at RT.
  • the panning strategy is similar to a standard solution panning described above.
  • the phage antigen complex was captured via the biotin moiety of C5 to pre-blocked Streptavidin magnetic beads 30 min at RT. Beads were then washed more stringently compared to a normal panning. Elution and amplification of phage was performed as described above.
  • the second and third rounds of selection were performed in an identical way to the first round, but at higher stringency washing conditions and lower antigen concentrations. For each antibody lead or pool several different pannings were performed. For each panning strategy different stringency conditions were applied. Panning strategies are summarized in Table 4.
  • the enriched phagemid pools were sub-cloned into pMORPH®x9_MH expression vector.
  • variable domain fragments of heavy (VH) and light chains (VL) were subcloned from the pMORPH®x9_MH Fab expression vectors into pMORPH®2_h_Ig vector series for human IgG2. Restriction enzymes Mfel, and Blpl were used for subcloning of the VH domain fragment into pMORPH®2_h_IgG2. Subcloning of the VL domain fragment into pMORPH®2_h_Ig ⁇ was performed via the EcoRV and BsiWl sites, whereas subcloning into pMORPH®2_h_Ig ⁇ 2 was done using EcoRV and Hpal.
  • variable domain fragments of Fab heavy (VH) and light chains (VL) were subcloned from the Fab expression vectors into IgG1 expression vectors.
  • Restriction enzymes Mfel, and Blpl were used for subcloning of the VH domain fragment into pMORPH®2_h_IgG1AA, in which leucines at positions 234 and 235 were mutated to alanines to abrogate FcR ⁇ binding and attenuate effector functions.
  • the restrictions enzymes EcoRV and Hpal were used to subclone of the VL domain fragment into pMORPH®2_h_Ig ⁇ 2.
  • Fabs with desired profile were subcloned into human IgG1AA format: MOR07832, 7834, 7872, 7876, 7829, 7871, 7865, 7873, 7830, 7878, 7910.
  • Cross-cloning on IgG level was achieved by transfecting cells with combinations of light and heavy chain constructs.
  • MOR08114 was the product of the germlined heavy chain from MOR07829 and the germlined light chain from MOR07871. Table 6 summarizes the most relevant cross-cloned germlined IgGs.
  • germlined VH VL matured VH matured VL format 8114 yes 7829 7871 7091/HCDR2 7091/LCDR3 hutg1AA 8125 yes 7091 7873 — 7091/LCDR3 hutg1AA 8126 yes 7829 7873 7091/HCDR2 7091/LCDR3 hutg1AA 8127 yes 7830 7873 7091/HCDR2 7091/LCDR3 hutg1AA 8128 yes 7092 7878 — 7092/LCDR3 hutg1AA 8129 yes 7909 7092 7092/HCDR2 — hutg1AA 8130 yes 7909 7878 7092/HCDR2 7092/LCDR3 hutg1AA 8131 yes 7910 7092 7092/HCDR2 — hutg1AA 8132 yes 7910 7878 70 7092/HCDR2 — hutg1AA 8132 yes 7910 7878 70
  • Eukaryotic HKB11 and HEK293 cells were transfected with an equimolar ratio of IgG heavy and light chain expression vector DNA.
  • Cell culture supernatant was harvested at 3 or 7 days post transfection and subjected to standard protein A affinity chromatography (rProteinA FF or MabSelect SURE, GE Healthcare).
  • buffer exchange was performed to 1 ⁇ Dulbcecco's PBS (pH 7.2, Invitrogen) and samples were sterile filtered (0.2 ⁇ m). Purity of IgG was analyzed under denaturing, reducing and non-reducing conditions in SDS-PAGE or by using Agilent BioAnalyzer and in native state by HP-SEC.
  • IgG constructs were germlined via site-directed mutagenesis using QuickChange® Site-Directed Mutagenesis Kit (Stratagene).
  • the N-terminal DI of MOR08111 V ⁇ 2 were changed to ES to match human germline sequence as well as to avoid a terminal Q (N-terminal Q can form pyroglutamine).
  • N-terminal DI of MOR08110 V ⁇ 3, MOR08113 V ⁇ 3, and MOR08114 V ⁇ 3 were germlined to SY, the most commonly found sequence in human ⁇ 3 genes.
  • N-terminal QVQ of MOR08111 VH2 was germlined to EVT to match a ⁇ 2 gene and avoid terminal Q.
  • N-terminal Q in MOR08109 VH5, MOR08110 VH5, MOR08113 VH5 and MOR08114 VH5 was also mutated to E.
  • Framework sequences for MOR08109 V ⁇ 3 were synthesized to match the human ⁇ 3j gene and cloned into the expression vector using Nhel and Hpal restriction sites. Sequence alignments of the antibodies variable domains with their respective closest related human germline sequences are shown in FIG. 1 .
  • a CM4 chip was coated with 50 ⁇ g/ml goat anti-human Fc antibody (500-2000 RU) in 10 mM acetate buffer, pH 4.5, using standard EDC-NHS amine coupling chemistry.
  • Each anti-human C5 IgG was captured on the chip in HBS-EP buffer at constant flow rate of 10 ⁇ l/min for a contact time leading to a ligand density around 20 RU.
  • different concentrations of human or cynomolgus C5 were injected.
  • Each cycle was completed with two regeneration steps with phosphoric acid. All running conditions were carried out at 25° C. in 1 ⁇ HBS-EP buffer.
  • the resulting signals were adjusted by double referencing, substracting the refraction index values from the reference flow cell and the binding step with no analyte. Data were collected at 10 Hz and analyzed using the Biacore T100 Evaluation Software Version 1.1 (GE). This program uses a global fitting analysis method for the determination of rate and affinity constants for each interaction.
  • the specificity of the antibodies were measured.
  • the Kon and Koff values for binding to human and cynomolgus C5 are as follows: Kon >1 ⁇ 10 5 , Koff ⁇ 1 ⁇ 10 4 ). These measurements were performed in Biacore for the germlined IgGs and resulting data are listed in Table 7.
  • Streptavidin MSD plates were coated with 0.2 ⁇ g/ml biotinylated human C5 (30 ⁇ l/well) and blocked with 5% BSA in PBS. Subsequently the equilibrated samples were transferred to those plates (30 ⁇ l per well) and incubated for 20 min. After washing, 30 ⁇ l/well of the ECL Sulfo-tag labeled detection antibody (goat anti-human (Fab)2) in a final dilution of 1:1500 was added to the MSD plate and incubated for 30 min on an Eppendorf shaker (700 rpm).
  • ECL Sulfo-tag labeled detection antibody goat anti-human (Fab)2
  • Electrochemiluminescence signals were detected using a Sector Imager 6000 (Meso Scale Discovery, Gaithersburg, Md., USA).
  • affinity of the parental Fabs to human C5 was determined. Because characterization focus was on efficacy in hemolytic assays, affinity measurements were done only for the most relevant Fabs. For a reliable determination of monovalent affinities only Fab batches were used for measurements which showed ⁇ 90% monomeric fraction in a qualitative size exclusion chromatography.
  • Affinities of the 10 parental Fabs which entered affinity maturation are summarized in Table 8. Affinities ranged from 72 pM to 3.7 nM.
  • Monovalent affinities of the purified Fabs to human C5 were measured in SET. Affinities were in the low pM range and best affinities were obtained for derivatives of MOR07086, 7091, 7092 and 7093. Subsequently affinity measurements of these derivatives to cynomolgus C5 showed affinities in the mid to low pM range.
  • Affinities of the germlined IgGs (human IgG1AA format) to human and cynomolgus C5 were determined in SET as described below. Similar data sets between two independent measurements showed higher affinities of the lead IgGs to human C5 than reference IgG 5G1.1 (see U.S. Pat. No. 6,355,245). Final IgGs had affinities for human C5 ranging from 1 to 14 pM and affinities to cynomolgus C5 ranging from 3 to 29 pM.
  • Streptavidin MSD plates were coated with 0.2 ⁇ g/ml biotinylated human C5 (30 ⁇ l/well) and blocked with 5% BSA in PBS. Subsequently the equilibrated samples were transferred to those plates (30 ⁇ l per well) and incubated for 20 min. After washing, 30 ⁇ l/well of the ECL Sulfo-tag labeled detection antibody (goat anti-human (Fab) 2 ) in a final dilution of 1:1500 was added to the MSD plate and incubated for 30 min on an Eppendorf shaker (700 rpm).
  • ECL Sulfo-tag labeled detection antibody goat anti-human (Fab) 2
  • Electrochemiluminescence signals were detected after washing and adding 30 ⁇ l/well MSD Read Buffer T with surfactant using a Sector Imager 6000 (Meso Scale Discovery, Gaithersburg, Md., USA).
  • the hemolytic assay is a basic functional assay that tests for complement activation and has been used to evaluate the ability of anti-human C5 mAbs and Fab molecules to block lysis of red blood cells (RBCS) by complement pathways (see e.g., Evans et al., Mol. Immunol 32: 1183-1195 (1995); Thomas et al., Mol Immunol 33:1389-1401 (1996); Rinder et al., J Clin Invest 96:1564-1572 (1995)).
  • RBCS red blood cells
  • sensitized red blood cells are used as targets for lysis by complement proteins present in serum. This assay is of interest for the characterization and screening of high-affinity anti-human C5 mAbs.
  • the desired number of chicken red blood cells was washed four times with cold gelatin veronal buffer (GVB++) and resuspended to 5 ⁇ 10 7 cells/ml.
  • GVB++ cold gelatin veronal buffer
  • rabbit anti-chRBC IgG was added to RBC cell suspension to a final concentration of 1 ⁇ g/ml IgG. After 15 minutes incubation on ice, the sensitized chRBCs were centrifuged, washed twice with GVB++ and diluted to 8.33 ⁇ 10 7 cells/ml.
  • control wells i) 0% lysis control ⁇ 100 ⁇ l GVB++, ii) 100% lysis control ⁇ 100 ⁇ l 0.1% NP-40, iii) 20% serum control ⁇ 100 ⁇ l of 20% serum (0% Ab control).
  • blank wells i) 20% serum blank ⁇ 100 ⁇ l 20% serum, ii) GVB++ blank ⁇ 100 ⁇ l GVB++, iii) NP-40 blank ⁇ 100 ⁇ l 0.1% NP-40.
  • % ⁇ ⁇ Hemolysis 100 ⁇ ODsample - ODnegativecontrol ODpositivecontrol - ODnegativecontrol
  • ODsample ⁇ AverageOD sample ⁇ - [ AverageOD 20 ⁇ % ⁇ SerumBlank ]
  • ODnegativecontrol ⁇ AverageOD 0 ⁇ % ⁇ ⁇ Lysis ⁇ - [ AverageOD GVB ++ ⁇ Blank ]
  • ODpositivecontrol ⁇ AverageOD 100 ⁇ % ⁇ ⁇ Lysis ⁇ - [ AverageOD NP - 40 ⁇ ⁇ Blank ]
  • Hemolytic assays undergoing the alternative pathway were done in a similar way to the classical pathway hemolytic assays.
  • RBCs cells from rabbit were used and there was no need to sensitize the cells.
  • the rabbit RBCs are different from chicken RBCs in that they are sensitive to lysis caused by the complement alternative pathway.
  • the working buffer was GVB++ supplemented with 10 mM EGTA and 5 mM Mg++, since the C5 convertase of the alternative pathway is Mg++ dependent and the C5 convertase of the classical pathway is Ca++ dependent.
  • Hemolytic assays of the alternative pathway were run with: i) 20% human serum, ii) 100 pM human C5 added to 20% human C5-depleted serum, iii) 0.025% cynomolgus serum added to 20% human C5-depleted serum, iv) 100 pM cynomolgus C5 added to 20% human C5-depleted serum, v) 10% cynomolgus serum.
  • These settings were used to screen for antibodies with high affinity to the human and cynomolgus C5 proteins which were able to inhibit very effectively the red blood cell lysis induced by the alternative complement pathway.
  • C5 convertase cleaves C5 into C5a peptide and C5b fragment, that is subsequently incorporated into the membrane-attack complex (MAC), which leads to cell lysis.
  • C5 convertase of the classical pathway formed by a C3bC4bC2a complex has a different structure than the C5 convertase of the alternative pathway which is formed by a C3bC3bBb complex.
  • HuCAL GOLD® generated antibodies should be inhibitory in both classical and alternative pathway, but with focus on the alternative pathway because mainly the alternative pathway (factor H, factor B and factor H-related genes) is implicated in AMD.
  • the classical and alternative pathway assays were performed with 20% human serum ( ⁇ 80 nM C5).
  • human serum ⁇ 80 nM C5
  • new assay formats were developed. 10-100 pM purified human C5 or 0.025% cynomolgus serum ( ⁇ 100 pM cynomolgus C5) were added to human C5-depleted serum (but containing all other serum and complement components).
  • FIG. 2 shows that considerable hemolysis could be observed between 10 and 100 pM purified human C5 added to human C5-depleted serum. Cynomolgus serum was added to human C5-depleted serum to test for cross-reactivity.
  • FIG. 3 shows that 0.025% of cynomolgus serum ( ⁇ 100 pM C5) added to human C5 depleted serum restores hemolytic activity.
  • FIG. 4 show an example with the best inhibitory Fabs.
  • Fabs which showed inhibitory activity in the classical pathway were further evaluated in the alternative pathway.
  • Hemolytic assays were run with 100 pM purified human C5 or 0.025% cynomolgus serum added to human C5-depleted serum.
  • IC 50 values for the human alternative assays were between 0.1 and 90 nM (examples of assays with the most relevant Fabs are shown in FIG. 5 .
  • FIG. 7 shows examples of hemolytic assays with derivatives of MOR07086, 7091, 7092 and 7093.
  • Matured Fabs were tested in the alternative pathway hemolytic assay with 100 pM human C5. Some derivatives of MOR06525, 6757, 6763, and 7087 showed potency improvement compared to their parentals. MOR07086-, 7091-, 7092-, 7093-, and 7094-derived Fabs showed highest potency (IC50 values in the low nM range). Descendants of MOR07091, 7092, 7093, and 7094 showed highly improved potency, many of which are more potent than reference antibody 5G1.1. FIG. 9 shows examples of hemolytic assay results for the affinity matured Fabs and 5G1.1.
  • FIG. 10 shows examples of hemolytic assay results for the affinity matured Fabs and reference antibody 5G1.1.
  • Matured Fabs were tested in the alternative pathway hemolytic assay using 100 pM cynomolgus C5 added to 20% human C5-depleted serum.
  • MOR07091-, 7092- and 7093-derived Fabs showed best inhibitory activity; 5G1.1 does not crossreact with cynomolgus C5.
  • FIG. 11 shows examples of hemolytic assay results for the affinity matured Fabs.
  • FIG. 14 shows examples of hemolytic assay results for the IgGs.
  • FIG. 15 shows examples of hemolytic assay results for the IgGs. Inhibition of C5a generation by the final IgGs was similar to 5G1.1 (IC50 values in the low nM range).
  • Hemolytic assays of the alternative pathway in 20% human C5-depleted serum were reconstituted with 100 pM cynomolgus C5. Potency of the germlined final candidates against cynomolgus C5 was within 5-fold of that for human C5 (IC50 values in the low pM range).
  • C5a-des-Arg ELISA was developed to measure C5a generation during hemolysis to confirm that antibodies that were inhibitory in the hemolytic assay also inhibited cleavage of C5 into C5a and C5b.
  • a Maxisorp plate was coated with 100 ⁇ l/well mouse anti-human C5a-des-Arg (US Biologics) at 1 ⁇ g/ml in coating buffer (bicarbonate pH 9.5-9.8) and was incubated overnight at 4° C. After washing 3 ⁇ with PBST, the plate was blocked with 300 ⁇ l/well diluent (Synblock, AbD Serotec) for 2 hours at room temperature. After aspirating the blocking solution, 100 ⁇ l samples or standards diluted with diluent were incubated for 1 hour at room temperature. Standards were prepared as follows: start was at 20 ng/ml standard (rC5a-des-Arg) and 1:4 serial dilutions were prepared for a 7-point curve.
  • FIG. 16 shows examples of C5a ELISA results.
  • Fabs were analyzed in a solution ELISA (method described above) for binding to human C3, C4 and C5. Fabs were incubated with biotinylated antigen on a Neutravidin plate and detected via the histidin tag.
  • Antibodies were incubated up to 8 h at 37° C. with 100% human C5-depleted serum or with PBST/0.5% BSA (positive control). Wells of a blocked polypropylene plate were used for incubation to ensure no binding of the antibodies to the surface over the long incubation time. Samples were collected at different time points and stored at ⁇ 20° C.
  • Fluorescence of the substrate was measured after 5-10 min and retained binding activity was calculated compared with the respective maximum signal (antibody incubated with PBST/0.5% BSA).
  • One of the “must” criteria for the C5-binding antibodies is to retain 75-80% of binding activity in human serum i) in a functional assay at 10% serum and ii) in a binding assay at 50% serum. Because hemolytic assays were run in the presence of 20% serum it was only necessary to show retained binding in a binding assay at 50% serum.
  • FIG. 18 illustrates the results of the final C5-binding final antibodies in the Fab format. 70-93% of the binding activity was retained after an 8 hour incubation time at 37° C. in 50% serum compared to incubation in PBS.
  • This procedure was used to group anti-human C5 Fabs into different epitope bins binding to the same or an overlapping epitope of the C5 protein.
  • Human C5 was captured via a polyclonal anti-human C5 IgG (US Biological), which was coated previously o/n at 4° C. on a 384 well black Maxisorp plates. Next day the plate was washed twice with PBST and blocked for 2 h with 3% BSA-PBST. After washing 3 ⁇ with PBST, 20 ⁇ l human C5 was added and incubated 2 h ar RT. The plate was washed 3 ⁇ with PBST before adding the Fabs.
  • US Biological polyclonal anti-human C5 IgG
  • Fabs were also divided into different epitope binding groups using a different method: FabX was immobilized, then FabY pre-incubated with biotinylated C5 was added. Following groups of Fabs could be defined: Group 1: MOR06952, 6961; Group 2: MOR06525,6757, 7083; Group 3: MOR07087; Group 4: MOR06763; Group 5: MOR07081; Group 6: MOR07082, 7083, 7084, 7086, 7088, 7091, 7092, 7093 (7089 competes with 7084). The conclusion was drawn that using two different methods, similar results could be obtained.
  • biotinylated Fabs compete with identical unlabelled Fabs and all Fabs compete for binding to the same or overlapping epitope. These results correlate with epitope binning data for the parental Fabs.
  • Fab was coated on a plate and purified C5 or supernatant from chimeric C5 preparation (human alpha, mouse beta chain) was added.
  • 5G1.1 was applied and detection was done via an anti-human IgG.
  • 5G1.1 was coated on a plate, purified C5 or supernatant from chimeric C5 preparation (human alpha, mouse beta chain) was added, then Fab, which was detected with an anti-Myc antibody.
  • Reference IgG 5G1.1 recognizes the alpha chain and was used to determine if the MorhpSys generated Fabs compete with 5G1.1 for binding.
  • supernatant from chimeric C5 preparation was added to human C5-depleted serum and Fabs were tested for inhibition of hemolysis.
  • FIG. 20 shows the results of an ELISA experiment where the Fabs were coated on a plate, C5 or supernatant of a chimeric C5 preparation (human alpha chain and mouse beta chain) was added, then 5G1.1.
  • FIG. 21 shows the results of an ELISA experiment where purified C5 and supernatant from chimeric C5 were captured via 5G1.1.
  • MOR06525, 6756, 6763 were beta chain binders (bind to C5 but not chimeric C5). Most MOR070XX Fabs (derived from solution pannings) are alpha chain binders (bind to C5 and chimeric C5). MOR06952 and 6961 compete with 5G1.1 so they are negative for both C5 and chimeric C5 and, thus, are most likely alpha chain binders as 5G1.1. MOR06757 behaves like MOR06952 and 6961, i.e. it likely is an alpha chain binder. However, MOR06757 does not inhibit hemolysis of chimeric C5 supernatant spiked into C5-depleted serum, while all the other alpha chain binders do (see FIG. 22 ).
  • the reaction was stopped by adding 4 ⁇ L of 0.5 M EDTA and 4 ⁇ L of 4 ⁇ LDS sample buffer (Invitrogen) and the stopped samples were run on 4-12% SDS-PAGE at non-reducing condition.
  • Proteolysis of Fabs was analyzed by monitoring the disappearance of Fab bands that were visualized by Coomassie staining.
  • Zymosan which is an insoluble carbohydrate from the cell wall of yeast, used especially in the immunoassay of the alternative pathway, was coated to activate the Alternative Pathway and IgM (Sigma) was coated to activate the Classical Pathway for determination of MAC (membrane attack complex) deposition.
  • Fabs were pre-incubated with human serum (6% for AP, 2% for CP) and added to plate. Percentage (%) inhibition of MAC deposition was calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum), and used to generate the IC 50 curve with XLFit.

Abstract

The present invention relates to antibodies targeting complement protein C5 and compositions and methods of use thereof.

Description

    1. INTRODUCTION
  • The present invention relates to antibodies targeting complement protein C5 and compositions and methods of use thereof.
    • 2. BACKGROUND OF THE INVENTION
  • The normal role of complement, which is part of the innate immune system, is in host defense. Complement defends against bacterial infection, links adaptive and innate immunity, and disposes immune complexes and the products of inflammatory injury.
  • The defensive functions are accomplished by biologically active products generated in the course of complement activation, which opsonise infectious agents, promote inflammation or lyse susceptible targets (Marzari et al., Eur J Immunol 32:2773-2782 (2002)). The complement system consists of about 25-30 plasma proteins which play a role in the immune system. The complement cascade is activated by at least three major pathways. The classical pathway is typically activated by immune-complexes, the alternative pathway can be activated by unprotected cell surfaces, and the mannose binding lectin (MBL) pathway is initiated by binding of MBL to cell surface carbohydrates (Trendelenburg, Swiss Med Wkly 137:413-417 (2007)).
  • All three pathways lead to the cleavage of C5 by the C5 convertase. The result of this cleavage is release of C5a fragment, a potent inflammatory molecule, and C5b which initiates the membrane attack complex (MAC). The complement products, once released, do not differentiate between foreign and self targets and, if not tightly regulated, often cause extensive damage of bystander cells and tissues in clinical conditions associated with unrestricted complement activation (Marzari et al., 2002).
  • C5 is expressed intracellularly as a single pro-05 peptide of 1676 amino acids that consist of an 18 residue signal sequence and an Arg-rich linker sequence (RPRR) situated between the mature N-terminal β chain and the C-terminal α chain. The mature C5 has a molecular weight of about 190 kDa, and consists of two polypeptide chains (α, 115 kDa and β, 75 kDa) which are connected by disulfide bonds. The C5 convertase cleaves C5 between residues 74 and 75 of the alpha chain to release the 74 amino acid C5a peptide and the C5b fragment which is subsequently incorporated into the membrane-attack complex (MAC).
  • Macular degeneration is a medical condition predominantly found in the elderly in which the center of the inner lining of the eye, known as the macula area of the retina, suffers thinning, atrophy, and in some cases, bleeding. This can result in loss of central vision, which entails inability to see fine details, to read, or to recognize faces. Pathogenesis of new choroidal vessel formation is poorly understood, but factors such as inflammation, ischemia, and local production of angiogenic factors are thought to be important.
  • Despite current treatment options for treating diseases and disorders associated with the classical or alternative component pathways, particularly AMD, there remains a need for finding specific targets that lead to treatments which are effective and well-tolerated.
    • 3. SUMMARY OF THE INVENTION
  • The present invention provides isolated complement C5-binding molecules (e.g., C5-binding antibodies or antigen binding fragments thereof), pharmaceutical compositions comprising such molecules, methods of making such molecules and compositions, and methods of use thereof.
  • In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibody has an affinity constant (KA) of at least 1×107 M−1, 108 M−1, 109 M−1, 1010 M−1, or 1011 M−1.
  • In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, and inhibit the alternative complete pathway as measured by in vitro hemolytic assay with an IC50 range from about 20 pM to about 200 pM.
  • In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, and cross compete with an antibody described in Table 1 below. In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that bind to the same epitope of C5 protein as an antibody described in Table 1 below.
  • In some embodiments, the antibodies of the invention are isolated monoclonal antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention are isolated human or humanized monoclonal antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention are isolated chimeric antibodies that specifically bind to a C5 protein. In some embodiments, the antibodies of the invention comprise a human heavy chain constant region and a human light chain constant region.
  • In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies are single chain antibodies. In some embodiments, the antibodies of the invention are Fab fragments. In some embodiments, the antibodies of the invention are scFv.
  • In some embodiments, the present invention provides isolated antibodies or antigen-binding fragments thereof that specifically bind to both human C5 and cynomolgus C5. In some embodiments, the antibodies of the invention are an IgG isotype.
  • In some embodiments, the present invention provides isolated antibodies or antigen binding fragments thereof comprising a framework in which amino acids have been substituted into the antibody framework from the respective human VH or VL germline sequences.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one complementarity determining (CDR) sequence having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 17, 18, 19, 20, 21, 22, 33, 34, 35, 36, 37, 38, 49, 50, 61, 62, 63, 64, 65, 66, 77, 78, 89, 95, 101, 107, 113, 119, 120, 131, 132, 133, 134, 135, 136, 145, 146, 147, 148, 149, 150, 159, 160, 161, 162, 163, 164, 173, 174, 175, 176, 177, 178, 195, 196, 197, 198, 199, 200, 209, 226, 235, 236, 237, 238, 239, or 240.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one heavy chain CDR sequence that is identical to SEQ ID NO: 1, 2, 3, 17, 18, 19, 33, 34, 35, 49, 61, 62, 63, 77, 77, 95, 107, 113, 119, 132, 131, 133, 145, 146, 147, 159, 160, 161, 173, 174, 175, 195, 196, 197, 226, 235, 236, or 237.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise at least one light chain CDR sequence that is identical to SEQ ID NO: 4, 5, 6, 20, 21, 22, 36, 37, 38, 50, 64, 65, 66, 78, 89, 101, 120, 134, 135, 136, 148, 149, 150, 162, 163, 164, 176, 177, 178, 198, 199, 200, 209, 238, 239, or 240.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain CDR 1 selected from the group consisting SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a heavy chain CDR2 selected from the group consisting SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; and a heavy chain CDR3 selected from the group consisting SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237. In some embodiments, such antibodies or antigen binding fragments thereof further comprise a light chain CDR1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a light chain CDR3 selected from the group consisting SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain CDR 1 selected from the group consisting SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain CDR2 selected from the group consisting SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a light chain CDR3 selected from the group consisting SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281. In some embodiments, such antibodies or antigen binding fragments thereof further comprise a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a heavy chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, or 282. In some embodiments, such antibodies further comprise a light chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, or 290.
  • In some embodiments, the present invention provides isolated monoclonal antibodies or antigen binding fragments thereof that specifically bind to a C5 protein, wherein said antibodies comprise a light chain having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, or 290.
  • The present invention also comprises pharmaceutical compositions comprising one or more C5-binding molecules of the invention (e.g., C5 binding antibodies or antigen binding fragments thereof) and a pharmaceutically acceptable carrier.
  • In some embodiments, the present invention provides nucleic acids comprising a nucleotide sequence encoding a polypeptide comprising a heavy chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281.
  • In some embodiments, the present invention provides nucleic acids comprising a nucleotide sequence encoding a polypeptide comprising a light chain variable region having at least 90%, 95%, 97%, 98% or at least 99% sequence identity to SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289.
  • The present invention also provides vectors and host cells comprising such nucleic acids. In one embodiment, the present invention provides isolated host cells comprising (1) a recombinant DNA segment encoding a heavy chain of the antibodies of the invention, and (2) a second recombinant DNA segment encoding a light chain of the antibodies of the invention; wherein said DNA segments are respectively operably linked to a first and a second promoter, and are capable of being expressed in said host cell. In another embodiment, the present invention provides isolated host cells comprising a recombinant DNA segment encoding a heavy chain, and a light chain of the antibodies of the invention, respectively, wherein said DNA segment is operably linked to a promoter, and is capable of being expressed in said host cells. In some embodiments, the host cells are non-human mammalian cell line. In some embodiments, the antibodies or antigen binding fragments thereof are a human monoclonal antibody, or an antigen binding fragment thereof.
  • The present invention further provides treatment of diagnostic methods using the C5 binding molecules (e.g., C5 binding antibodies or antigen binding fragments thereof) of the invention. In one embodiment, the present invention provides methods of treating age related macular degeneration comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or an antigen binding fragment thereof of the invention.
  • In another embodiment, the present invention provides methods of treating a disease comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or an antigen binding fragment thereof of the invention, wherein said disease is asthma, arthritis, autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer-Simons Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, Alzheimer's disease, glomerulonephritis, or MPGN II.
  • The present invention also provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or antigen binding fragment thereof of the invention.
  • The present invention further provides methods of ameliorating a symptom associated with extracorporeal circulation comprising administering to a subject in need thereof an effective amount of a composition comprising an antibody or antigen binding fragment thereof of the invention.
    • 3.1. Definitions
  • Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention pertains.
  • The term “antibody” as used herein includes whole antibodies and any antigen binding fragment (i.e., “antigen-binding portion”) or single chains thereof. A naturally occurring “antibody” is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds. Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region. The heavy chain constant region is comprised of three domains, CH1, CH2 and CH3. Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system.
  • The term “antigen binding portion” of an antibody, as used herein, refers to one or more fragments of an intact antibody that retain the ability to specifically bind to a given antigen (e.g., C5). Antigen binding functions of an antibody can be performed by fragments of an intact antibody. Examples of binding fragments encompassed within the term “antigen binding portion” of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; an Fd fragment consisting of the VH and CH1 domains; an Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a single domain antibody (dAb) fragment (Ward et al., 1989 Nature 341:544-546), which consists of a VH domain; and an isolated complementarity determining region (CDR).
  • Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by an artificial peptide linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see, e.g., Bird et al., 1988 Science 242:423-426; and Huston et al., 1988 Proc. Natl. Acad. Sci. 85:5879-5883). Such single chain antibodies include one or more “antigen binding portions” of an antibody. These antibody fragments are obtained using conventional techniques known to those of skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.
  • Antigen binding portions can also be incorporated into single domain antibodies, maxibodies, minibodies, intrabodies, diabodies, triabodies, tetrabodies, v-NAR and bis-scFv (see, e.g., Hollinger and Hudson, 2005, Nature Biotechnology, 23, 9, 1126-1136). Antigen binding portions of antibodies can be grafted into scaffolds based on polypeptides such as Fibronectin type III (Fn3) (see U.S. Pat. No. 6,703,199, which describes fibronectin polypeptide monobodies).
  • Antigen binding portions can be incorporated into single chain molecules comprising a pair of tandem Fv segments (VH-CH1-VH-CH1) which, together with complementary light chain polypeptides, form a pair of antigen binding regions (Zapata et al., 1995 Protein Eng. 8(10):1057-1062; and U.S. Pat. No. 5,641,870).
  • As used herein, the term “Affinity” refers to the strength of interaction between antibody and antigen at single antigenic sites. Within each antigenic site, the variable region of the antibody “arm” interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the affinity.
  • As used herein, the term “Avidity” refers to an informative measure of the overall stability or strength of the antibody-antigen complex. It is controlled by three major factors: antibody epitope affinity; the valency of both the antigen and antibody; and the structural arrangement of the interacting parts. Ultimately these factors define the specificity of the antibody, that is, the likelihood that the particular antibody is binding to a precise antigen epitope.
  • The term “amino acid” refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, γ-carboxyglutamate, and O-phosphoserine. Amino acid analogs refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an alpha carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid.
  • The term “binding specificity” as used herein refers to the ability of an individual antibody combining site to react with only one antigenic determinant. The combining site of the antibody is located in the Fab portion of the molecule and is constructed from the hypervariable regions of the heavy and light chains. Binding affinity of an antibody is the strength of the reaction between a single antigenic determinant and a single combining site on the antibody. It is the sum of the attractive and repulsive forces operating between the antigenic determinant and the combining site of the antibody.
  • Specific binding between two entities means a binding with an equilibrium constant (KA) of at least 1×107 M−1, 108 M−1, 109 M−1, 1010 M−1, or 1011 M. The phrase “specifically (or selectively) binds” to an antibody (e.g., a C5-binding antibody) refers to a binding reaction that is determinative of the presence of a cognate antigen (e.g., a human C5 or cynomolgus C5) in a heterogeneous population of proteins and other biologics. In addition to the equilibrium constant (KA) noted above, an C5-binding antibody of the invention typically also has a dissociation rate constant (Kd) of about 1×10−2 s−1, 1×10−3 s−1, 1×10−4 s−1, 1×10−4 s−1, or lower, and binds to C5 with an affinity that is at least two-fold greater than its affinity for binding to a non-specific antigen (e.g., C3, C4, BSA). The phrases “an antibody recognizing an antigen” and “an antibody specific for an antigen” are used interchangeably herein with the term “an antibody which binds specifically to an antigen”.
  • The term “chimeric antibody” is an antibody molecule in which (a) the constant region, or a portion thereof, is altered, replaced or exchanged so that the antigen binding site (variable region) is linked to a constant region of a different or altered class, effector function and/or species, or an entirely different molecule which confers new properties to the chimeric antibody, e.g., an enzyme, toxin, hormone, growth factor, drug, etc.; or (b) the variable region, or a portion thereof, is altered, replaced or exchanged with a variable region having a different or altered antigen specificity. For example, a mouse antibody can be modified by replacing its constant region with the constant region from a human immunoglobulin. Due to the replacement with a human constant region, the chimeric antibody can retain its specificity in recognizing the antigen while having reduced antigenicity in human as compared to the original mouse antibody.
  • The term “complement C5 protein” or “C5” are used interchangeably, and refers to the C5 protein in different species. For example, human C5 has the sequence as set in SEQ ID NO: 296, cynomolgus C5 has the sequence as set in SEQ ID NO: 297 (Macaca fascicularis) (see Table 1). Human C5 can be obtained from Quidel (Cat. Number A403). Cynomolgus C5 can be produced as illustrated in the Example section below.
  • The term “conservatively modified variant” applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide. Such nucleic acid variations are “silent variations,” which are one species of conservatively modified variations. Every nucleic acid sequence herein which encodes a polypeptide also describes every possible silent variation of the nucleic acid. One of skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid that encodes a polypeptide is implicit in each described sequence.
  • For polypeptide sequences, “conservatively modified variants” include individual substitutions, deletions or additions to a polypeptide sequence which result in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention. The following eight groups contain amino acids that are conservative substitutions for one another: 1) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)). In some embodiments, the term “conservative sequence modifications” are used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence.
  • The terms “cross-block”, “cross-blocked” and “cross-blocking” are used interchangeably herein to mean the ability of an antibody or other binding agent to interfere with the binding of other antibodies or binding agents to C5 in a standard competitive binding assay.
  • The ability or extent to which an antibody or other binding agent is able to interfere with the binding of another antibody or binding molecule to C5, and therefore whether it can be said to cross-block according to the invention, can be determined using standard competition binding assays. One suitable assay involves the use of the Biacore technology (e.g. by using the BIAcore 3000 instrument (Biacore, Uppsala, Sweden)), which can measure the extent of interactions using surface plasmon resonance technology. Another assay for measuring cross-blocking uses an ELISA-based approach.
  • The term “epitope” means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.
  • As used herein, the term “high affinity” for an IgG antibody refers to an antibody having a KD of 10−8 M or less, 10−9 M or less, or 10−10 M, or 10−11 M or less for a target antigen. However, “high affinity” binding can vary for other antibody isotypes. For example, “high affinity” binding for an IgM isotype refers to an antibody having a KD of 10−7 M or less, or 10−8 M or less.
  • The term “human antibody”, as used herein, is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from sequences of human origin. Furthermore, if the antibody contains a constant region, the constant region also is derived from such human sequences, e.g., human germline sequences, or mutated versions of human germline sequences. The human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
  • The term “human monoclonal antibody” refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences. In one embodiment, the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
  • A “humanized” antibody is an antibody that retains the reactivity of a non-human antibody while being less immunogenic in humans. This can be achieved, for instance, by retaining the non-human CDR regions and replacing the remaining parts of the antibody with their human counterparts (i.e., the constant region as well as the framework portions of the variable region). See, e.g., Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855, 1984; Morrison and Oi, Adv. Immunol., 44:65-92, 1988; Verhoeyen et al., Science, 239:1534-1536, 1988; Padlan, Molec. Immun., 28:489-498, 1991; and Padlan, Molec. Immun., 31:169-217, 1994. Other examples of human engineering technology include, but is not limited to Xoma technology disclosed in U.S. Pat. No. 5,766,886.
  • The terms “identical” or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same. Two sequences are “substantially identical” if two sequences have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 60% identity, optionally 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity over a specified region, or, when not specified, over the entire sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Optionally, the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length.
  • For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters.
  • A “comparison window”, as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443, 1970, by the search for similarity method of Pearson and Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444, 1988, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection (see, e.g., Brent et al., Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (ringbou ed., 2003)).
  • Two examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., Nuc. Acids Res. 25:3389-3402, 1977; and Altschul et al., J. Mol. Biol. 215:403-410, 1990, respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) or 10, M=5, N=−4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915, 1989) alignments (B) of 50, expectation (E) of 10, M=5, N=−4, and a comparison of both strands.
  • The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787, 1993). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001.
  • The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4:11-17, 1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol, Biol. 48:444-453, 1970) algorithm which has been incorporated into the GAP program in the GCG software package (available at www.gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6.
  • Other than percentage of sequence identity noted above, another indication that two nucleic acid sequences or polypeptides are substantially identical is that the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the antibodies raised against the polypeptide encoded by the second nucleic acid, as described below. Thus, a polypeptide is typically substantially identical to a second polypeptide, for example, where the two peptides differ only by conservative substitutions. Another indication that two nucleic acid sequences are substantially identical is that the two molecules or their complements hybridize to each other under stringent conditions, as described below. Yet another indication that two nucleic acid sequences are substantially identical is that the same primers can be used to amplify the sequence.
  • The term “isolated antibody” refers to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds C5 is substantially free of antibodies that specifically bind antigens other than C5). An isolated antibody that specifically binds C5 may, however, have cross-reactivity to other antigens. Moreover, an isolated antibody may be substantially free of other cellular material and/or chemicals.
  • The term “isotype” refers to the antibody class (e.g., IgM, IgE, IgG such as IgG1 or IgG4) that is provided by the heavy chain constant region genes. Isotype also includes modified versions of one of these classes, where modifications have been made to alter the Fc function, for example, to enhance or reduce effector functions or binding to Fc receptors.
  • The term “Kassoc” or “Ka”, as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction, whereas the term “Kdis” or “Kd,” as used herein, is intended to refer to the dissociation rate of a particular antibody-antigen interaction. The term “KD”, as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/Ka) and is expressed as a molar concentration (M). KD values for antibodies can be determined using methods well established in the art. A method for determining the KD of an antibody is by using surface plasmon resonance, or using a biosensor system such as a Biacore® system.
  • The terms “monoclonal antibody” or “monoclonal antibody composition” as used herein refer to a preparation of antibody molecules of single molecular composition. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.
  • The term “nucleic acid” is used herein interchangeably with the term “polynucleotide” and refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs).
  • Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences, as well as the sequence explicitly indicated. Specifically, as detailed below, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081, 1991; Ohtsuka et al., J. Biol. Chem. 260:2605-2608, 1985; and Rossolini et al., Mol. Cell. Probes 8:91-98, 1994).
  • The term “operably linked” refers to a functional relationship between two or more polynucleotide (e.g., DNA) segments. Typically, it refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence. For example, a promoter or enhancer sequence is operably linked to a coding sequence if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system. Generally, promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting. However, some transcriptional regulatory sequences, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.
  • As used herein, the term, “optimized” means that a nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the production cell or organism, generally a eukaryotic cell, for example, a cell of Pichia, a Chinese Hamster Ovary cell (CHO) or a human cell. The optimized nucleotide sequence is engineered to retain completely or as much as possible the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the “parental” sequence. The optimized sequences herein have been engineered to have codons that are preferred in mammalian cells. However, optimized expression of these sequences in other eukaryotic cells or prokaryotic cells is also envisioned herein. The amino acid sequences encoded by optimized nucleotide sequences are also referred to as optimized.
  • The terms “polypeptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non-naturally occurring amino acid polymer. Unless otherwise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.
  • The term “recombinant human antibody”, as used herein, includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene, sequences to other DNA sequences. Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences. In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
  • The term “recombinant host cell” (or simply “host cell”) refers to a cell into which a recombinant expression vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein.
  • The term “subject” includes human and non-human animals. Non-human animals include all vertebrates, e.g., mammals and non-mammals, such as non-human primates, sheep, dog, cow, chickens, amphibians, and reptiles. Except when noted, the terms “patient” or “subject” are used herein interchangeably.
  • The term “treating” includes the administration of compositions or antibodies to prevent or delay the onset of the symptoms, complications, or biochemical indicia of a disease (e.g., AMD), alleviating the symptoms or arresting or inhibiting further development of the disease, condition, or disorder. Treatment may be prophylactic (to prevent or delay the onset of the disease, or to prevent the manifestation of clinical or subclinical symptoms thereof) or therapeutic suppression or alleviation of symptoms after the manifestation of the disease.
  • The term “vector” is intended to refer to a polynucleotide molecule capable of transporting another polynucleotide to which it has been linked. One type of vector is a “plasmid”, which refers to a circular double stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply, “expression vectors”). In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” may be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
    • 4. BRIEF DESCRIPTION OF THE FIGURES
  • FIG. 1 shows variable-region alignments of selected antibodies with their most closely related human germline sequences.
  • FIG. 2 shows a hemolytic assay in which human C5 is titrated into human C5-depleted serum to determine C5 activity.
  • FIG. 3 shows titration of cynomolgus serum into human C5-depleted serum to determine optimal cynomolgus C5 concentration for alternative pathway hemolytic assay.
  • FIG. 4 shows examples of classical pathway hemolytic assays with 20% human serum.
  • FIG. 5 shows example of alternative pathway hemolytic assays with 100 pM purified human C5 added to human C5-depleted serum.
  • FIG. 6 shows examples of alternative pathway hemolytic assays with 0.025% cynomolgus serum added to human C5-depleted serum.
  • FIG. 7 shows examples of classical pathway hemolytic assays (20% human serum) with matured Fabs in comparison to their respective parentals.
  • FIG. 8 shows examples of classical pathway hemolytic assays (5% cynomolgus serum) with matured Fabs.
  • FIG. 9 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 100 pM human C5 added to 20% human C5-depleted serum.
  • FIG. 10 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 20% human serum.
  • FIG. 11 shows affinity matured Fab characterization in alternative pathway hemolytic assay using 100 pM cynomolgus C5 added to 20% human C5-depleted serum.
  • FIG. 12 shows characterization of germlined IgGs in classical pathway hemolytic assay using 20% human serum.
  • FIG. 13 shows characterization of germlined IgGs in classical pathway hemolytic assay using 5% cynomolgus serum.
  • FIG. 14 shows characterization of germlined IgGs in alternative pathway hemolytic assay, 100 pM human C5.
  • FIG. 15 shows characterization of final germlined IgGs in alternative pathway hemolytic assay and C5a generation ELISA using 20% human serum.
  • FIG. 16 shows affinity matured Fab characterization in the C5a ELISA using supernatant from 20% human serum hemolytic assays.
  • FIG. 17 shows specificity solution ELISA on human C3, C4, C5 and cynomolgus C5 testing antibody 7091 and its derivatives.
  • FIG. 18 shows serum stability assays (binding to human C5 in the presence of 50% serum) with the Fabs.
  • FIG. 19 shows epitope binning of some affinity improved Fabs.
  • FIG. 20 shows an ELISA for antibody binding to mouse-human chimeric C5 or human C5 to determine alpha chain versus beta chain binders. C5 was presented by 5G1.1 to determine competition with 5G1.1.
  • FIG. 21 shows ELISA for testing alpha chain versus beta chain binders with 5G1.1 capture.
  • FIG. 22 shows results of hemolytic assay for testing alpha chain versus beta chain binders.
  • FIG. 23 show thermolysin proteolysis of parental Fabs at 37° C. (0, 30, 60 and 90 minutes).
  • FIG. 24 show thermolysin proteolysis of parental Fabs at 55° C. (0, 30, 60 and 90 minutes).
  • FIG. 25 shows thermolysin sensitivity of matured Fabs at 37° C.
  • FIG. 26 shows thermolysin sensitivity of matured Fabs at 55° C.
  • FIG. 27 shows examples of Fab inhibition of alternative pathway in MAC deposition assay.
    •  5. DETAILED DESCRIPTION OF THE INVENTION
  • The present invention provides antibodies that specifically bind to complement C5 protein (e.g., human C5, cynomologus C5), pharmaceutical compositions, production methods, and methods of use of such antibodies and compositions.
    • 5.1. C5 Antibodies
  • The present invention provides antibodies that specifically bind to C5 (e.g., human C5, cynomologus C5). In some embodiments, the present invention provides antibodies that specifically bind to both human and cynomologus C5. Antibodies of the invention include, but are not limited to, the human monoclonal antibodies, isolated as described, in the Examples (see Section 6 below).
  • The present invention provides antibodies that specifically bind a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VH domain having an amino acid sequence of SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281. The present invention also provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VH CDR having an amino acid sequence of any one of the VH CDRs listed in Table 1, infra. In particular, the invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising (or alternatively, consisting of) one, two, three, four, five or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1, infra.
  • The present invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VL domain having an amino acid sequence of SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289. The present invention also provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising a VL CDR having an amino acid sequence of any one of the VL CDRs listed in Table 1, infra. In particular, the invention provides antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5), said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1, infra.
  • Other antibodies of the invention include amino acids that have been mutated, yet have at least 60, 70, 80, 90 or 95 percent identity in the CDR regions with the CDR regions depicted in the sequences described in Table 1. In some embodiments, it includes mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the CDR regions when compared with the CDR regions depicted in the sequence described in Table 1.
  • The present invention also provides nucleic acid sequences that encode VH, VL, the full length heavy chain, and the full length light chain of the antibodies that specifically bind to a C5 protein (e.g., human and/or cynomologus C5). Such nucleic acid sequences can be optimized for expression in mammalian cells (for example, Table 1 shows the optimized nucleic acid sequences for the heavy chain and light chain of antibodies 8109, 8110, 8111, 8113, 8114, 8112, 8125, 8126, 8127, 8128, 8129, 8130, 8131, 8132, and 8091).
  • TABLE 1
    Examples of C5 Antibodies of the Present Invention and C5 Proteins
    Sequence Identifier (SEQ ID NO:) or comments/details
    Antibody
    8109
    CDRH1 1: SYAIS
    CDRH2 2: GIGPFFGTANYAQKFQG
    CDRH3 3: DTPYFDY
    CDRL1 4: SGDSIPNYYVY
    CDRL2 5: DDSNRPS
    CDRL3 6: QSFDSSLNAEV
    VH 7: EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQGRVTITADE
    STSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSS
    VL 8: SYELTQPLSVSVALGQTARITCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTISRA
    QAGDEADYYCQSFDSSLNAEVFGGGTKLTVL
    Heavy chain 9: EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQGRVTITADE
    STSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPC
    PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV
    LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE
    SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 10: SYELTQPLSVSVALGQTARITCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTISR
    AQAGDEADYYCQSFDSSLNAEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
    SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 11: GAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID NO: 7 CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    GGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 12: TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCTGCTCCG
    SEQ ID NO: 8 GCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATCTA
    CGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACCC
    TGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTACTACTGCCAGAGCTTCGACAGCAGCCTGAACGC
    CGAGGTGTTCGGCGGAGGGACCAAGTTAACCGTCCTA
    PN encoding 13: GAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID NO: 9 CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    GGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGT
    CCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTC
    AAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC
    CCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCA
    CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATC
    TTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTC
    CCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCC
    ACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCG
    GGAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGG
    CAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAA
    GGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGC
    CTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAG
    AACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGG
    ACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACAC
    GCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 14: TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCTGCTCCG
    SEQ ID NO: 10 GCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATCTA
    CGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACCC
    TGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTACTACTGCCAGAGCTTCGACAGCAGCCTGAACGC
    CGAGGTGTTCGGCGGAGGGACCAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC
    CCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAG
    CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAAC
    AAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTA
    CAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 15: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAGAAGCCCGGTAGCAGCGTCAAGGTGTCCTGCAAG
    encoding SEQ GCCAGCGGCGGCACCTTCAGCAGCTACGCCATCAGCTGGGTGCGGCAGGCCCCAGGCCAGGGCCTGGAGTG
    ID NO: 9 GATGGGCGGCATCGGCCCATTCTTCGGCACCGCCAACTACGCCCAGAAGTTCCAGGGCAGGGTCACCATCAC
    CGCCGACGAGAGCACCAGCACCGCCTACATGGAGCTGTCCAGCCTGAGAAGCGAGGACACCGCCGTGTACTA
    CTGCGCCAGAGACACCCCCTACTTCGACTACTGGGGCCAGGGCACCCTGGTGACCGTGAGCAGCGCTAGCAC
    CAAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCT
    GCCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGC
    ACACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCA
    GCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGA
    GCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGT
    GTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGT
    GGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAA
    GACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGA
    CTGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATC
    AGCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
    AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAAC
    GGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCA
    AGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGC
    ACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 16: AGCTACGAGCTGACCCAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCAGGATCACCTGCAGCG
    encoding SEQ GCGACAGCATCCCCAACTACTACGTGTACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATCTA
    ID NO: 10 CGACGACAGCAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACCCT
    GACCATCAGCAGAGCCCAGGCCGGCGACGAGGCCGACTACTACTGCCAGAGCTTCGACAGCTCACTGAACGC
    CGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTT
    CCCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGG
    CGCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCA
    AGCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGT
    CCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8110
    CDRH1 17: NYIS
    CDRH2 18: IIDPDDSYTEYSPSFQG
    CDRH3 19: YEYGGFDI
    CDRL1 20: SGDNIGNSYVH
    CDRL2 21: KDNDRPS
    CDRL3 22: GTYDIESYV
    VH 23: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQVTISADKSI
    STAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL 24: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCGTYDIESYVFGGGTKLTVL
    Heavy chain 25: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQVTISADKSI
    STAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPC
    PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV
    LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE
    SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 26: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCGTYDIESYVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADS
    SPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 27: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 23 TTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGCA
    TTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAAG
    CATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTATG
    AGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 28: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 24 CGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGGTACTTATGATATTGAGTCTTATGTGTTTGG
    CGGCGGCACGAAGTTAACCGTCCTA
    PN encoding 29: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 25 TTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGCA
    TTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAAG
    CATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTATG
    AGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATC
    GGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGA
    CTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGC
    TGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAG
    ACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA
    CAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCCCCCA
    AAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAG
    ACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG
    AGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGG
    AGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCA
    GCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGAC
    CTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAA
    CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAG
    AGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGA
    AGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 30: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 26 CGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGGTACTTATGATATTGAGTCTTATGTGTTTGG
    CGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCT
    GAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGG
    CCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA
    AGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGG
    TCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 31: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAGG
    encoding SEQ GCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGATGG
    ID NO: 25 GCATCATCGACCCCGACGACAGCTACACCGAGTACAGCCCCAGCTTCCAGGGCCAGGTGACCATCAGCGCCG
    ACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGCG
    CCAGATACGAGTACGGCGGCTTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCA
    AGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGC
    CTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCAC
    ACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAGC
    CTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAGC
    CCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTGT
    TCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTGG
    ACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGA
    CCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACT
    GGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAG
    CAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGAA
    CCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACGG
    CCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAAG
    CTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCAC
    AACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 32: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAACATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 26 TACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACTCCGGCAACACCGCCACC
    CTGACCATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCGGCACCTACGACATCGAGTCATAC
    GTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCC
    CCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCC
    GTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCA
    GAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTCCTA
    CAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8111
    CDRH1 33: TSGGGVS
    CDRH2 34: NIDDADIKDYSPSLKS
    CDRH3 35: GPYGFDS
    CDRL1 36: TGTSSDIGTYNYVS
    CDRL2 37: DDSNRPS
    CDRL3 38: QSYDSQSIV
    VH 39: EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLANIDDADIKDYSPSLKSRLTISKDTSK
    NQVVLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSS
    VL 40: ESALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSKSGNTASL
    TISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVL
    Heavy chain 41: EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLANIDDADIKDYSPSLKSRLTISKDTSK
    NQVVLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEP
    VTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCP
    APEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL
    TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWES
    NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 42: ESALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSKSGNTASL
    TISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 43: GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCTT
    SEQ ID NO: 39 TTCCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAGT
    GGCTGGCTAATATTGATGATGCTGATATTAAGGATTATTCTCCTTCTCTTAAGTCTCGTCTGACCATTAGCAAAGA
    TACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCGCGC
    GTGGTCCTTATGGTTTTGATTCTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 44: GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGGG
    SEQ ID NO: 40 TACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTTA
    TGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGCG
    AGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATTCTCAGTCTAT
    TGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding 45: GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCTT
    SEQ ID NO: 41 TTCCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAGT
    GGCTGGCTAATATTGATGATGCTGATATTAAGGATTATTCTCCTTCTCTTAAGTCTCGTCTGACCATTAGCAAAGA
    TACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCGCGC
    GTGGTCCTTATGGTTTTGATTCTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCC
    ATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAA
    GGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCC
    GGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACC
    CAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTG
    TGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCCC
    CCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACG
    AAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGG
    AGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCA
    AGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGG
    GCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCT
    GACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAA
    CAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGAC
    AAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGC
    AGAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 46: GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGGG
    SEQ ID NO: 42 TACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTTA
    TGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGCG
    AGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATTCTCAGTCTAT
    TGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG
    CCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCG
    TGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA
    GCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAG
    CTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 47: GAGGTGACCCTGAAGGAGAGCGGCCCAGCCCTGGTGAAGCCCACCCAGACCCTGACCCTGACTTGCACCT
    encoding SEQ TCAGCGGCTTCAGCCTGAGCACCAGCGGAGGGGGCGTGAGCTGGATCAGGCAGCCCCCAGGTAAGGCCCTG
    ID NO: 41 GAGTGGCTGGCCAATATCGACGACGCCGATATCAAGGACTACAGCCCCAGCCTGAAGAGCAGGCTGACCATCA
    GCAAGGACACCAGCAAGAACCAGGTGGTGCTGACCATGACCAATATGGACCCCGTGGACACCGCCACCTACTA
    CTGCGCCAGAGGCCCCTACGGCTTCGACAGCTGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCAC
    CAAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCT
    GCCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGC
    ACACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCA
    GCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGA
    GCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGT
    GTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGT
    GGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAA
    GACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGA
    CTGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATC
    AGCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
    AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAAC
    GGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCA
    AGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGC
    ACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 48: GAGAGCGCCCTGACCCAGCCCGCCAGCGTGAGCGGCAGCCCAGGCCAGTCTATCACAATCAGCTGCACCG
    encoding SEQ GCACCTCCAGCGATATCGGCACCTACAACTACGTGAGCTGGTATCAGCAGCACCCCGGCAAGGCCCCCAAGCT
    ID NO: 42 GATGATCTACGACGACAGCAACAGGCCCAGCGGCGTGAGCAACAGGTTCAGCGGCAGCAAGAGCGGCAACAC
    CGCCAGCCTGACAATCAGCGGCCTGCAGGCCGAGGACGAGGCCGACTACTACTGCCAGAGCTACGACAGCCA
    GTCAATCGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCT
    GTTCCCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCC
    AGGCGCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCA
    GCAAGCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACA
    GGTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8113
    CDRH1 SEQ ID NO: 17
    CDRH2 49: IIDPDDSYTRYSPSFQG
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 50: ATWGSEDQV
    VH 51: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQVTISADKSI
    STAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL 52: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCATWGSEDQVFGGGTKLTVL
    Heavy chain 53: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQVTISADKSI
    STAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPC
    PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV
    LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAMEWE
    SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 54: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCATWGSEDQVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
    SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 55: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 51 TTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGCA
    TTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGGACAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 56: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 52 CGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGCTACTTGGGGTTCTGAGGATCAGGTGTTTG
    GCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding 57: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 53 TTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGCA
    TTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGGACAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCA
    TCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG
    GACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCG
    GCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCC
    AGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
    GACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCCCC
    CAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGA
    AGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGA
    GGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAA
    GGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGG
    CAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTG
    ACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAAC
    AACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACA
    AGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCA
    GAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 58: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 54 CGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGCTACTTGGGGTTCTGAGGATCAGGTGTTTG
    GCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTC
    TGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTG
    GCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAAC
    AAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAG
    GTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 59: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAGG
    encoding SEQ GCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGATGG
    ID NO: 53 GCATCATCGACCCCGACGACAGCTACACCAGGTACAGCCCCAGCTTCCAGGGCCAGGTGACCATCAGCGCCG
    ACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGCG
    CCAGATACGAGTACGGCGGCTTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCA
    AGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGC
    CTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCAC
    ACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAGC
    CTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAGC
    CCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTGT
    TCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTGG
    ACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGA
    CCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACT
    GGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAG
    CAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGAA
    CCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACGG
    CCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAAG
    CTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCAC
    AACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 60: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAATATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 54 TACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACTCCGGCAACACCGCCACC
    CTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCGCCACCTGGGGCTCAGAGGACCAG
    GTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCC
    CCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCC
    GTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCA
    GAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTCCTA
    CAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8114
    CDRH1 61: SYYIG
    CDRH2 62: IIDPTDSQTAYSPSFQG
    CDRH3 63: YMMRGFDH
    CDRL1 64: SGDSLGDYYAY
    CDRL2 65: KDNNRPS
    CDRL3 66: QTWDTGESGV
    VH 67: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL 68: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDTGESGVFGGGTKLTVL
    Heavy chain 69: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPP
    CPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS
    VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEW
    ESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 70: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDTGESGVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 71: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 67 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATA
    AAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGT
    TATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 72: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 68 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATACTGGTGAGTCTGGTGTGTT
    TGGCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding 73: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 69 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATA
    AAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGT
    TATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTC
    CATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCA
    AGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCC
    CGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCAC
    CCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTT
    GTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCC
    CCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCAC
    GAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGG
    GAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGC
    AAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAG
    GGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC
    TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGA
    ACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA
    CAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACG
    CAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 74: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 70 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATACTGGTGAGTCTGGTGTGTT
    TGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC
    TCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG
    TGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACA
    ACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCA
    GGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 75: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAGG
    encoding SEQ GCAGCGGCTACAGCTTCACCAGCTACTACATCGGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGA
    ID NO: 69 TGGGCATCATCGACCCCACCGACAGCCAGACCGCCTACAGCCCCAGCTTCCAGGGCCAGGTGACCATCAGCG
    CCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACT
    GCGCCCGGTACATGATGAGGGGCTTCGACCACTGGGGTCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCA
    CCAAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGC
    TGCCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTG
    CACACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGC
    AGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGG
    AGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCG
    TGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGT
    GGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAA
    GACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGA
    CTGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATC
    AGCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
    AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAAC
    GGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCA
    AGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGC
    ACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 76: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAGCCTGGGCGACTACTACGCCTACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 70 TACAAGGACAACAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACC
    CTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCCAGACCTGGGACACCGGCGAGTCA
    GGCGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGTCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTC
    CCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGC
    GCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAA
    GCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTC
    CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8112
    CDRH1 SEQ ID NO: 61
    CDRH2 77: IIDPSDSHTTYSPSFQG
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 78: QTWDILPHGLV
    VH 79: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPSDSHTTYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL 80: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYC QTWDILPHGLVFGGGTKLTVL
    Heavy chain 81: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPSDSHTTYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPP
    CPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS
    VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEW
    ESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 82: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDILPHGLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 83: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 79 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATCGATCCGTCTGATAGCCATACCACTTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGAT
    AAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 84: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 80 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATATTCTTCCTCATGGTCTTGT
    GTTTGGCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding 85: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 81 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATCGATCCGTCTGATAGCCATACCACTTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGAT
    AAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGG
    TCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGT
    CAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTT
    CCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGC
    ACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAAT
    CTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTT
    CCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC
    CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGC
    GGGAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATG
    GCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAA
    AGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG
    CCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA
    GAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTG
    GACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACA
    CGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding 86: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 82 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATATTCTTCCTCATGGTCTTGT
    GTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCC
    TCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGA
    CAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCA
    ACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTG
    CCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 87: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAGG
    encoding SEQ GCAGCGGCTACAGCTTCACCAGCTACTACATCGGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGA
    ID NO: 81 TGGGCATTATCGATCCGTCTGATAGCCATACCACTTATTCTCCGAGCTTTCAGGGCCAGGTGACCATCAGCGCC
    GACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC
    GCCCGGTACATGATGAGGGGCTTCGACCACTGGGGTCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACC
    AAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTG
    CCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCA
    CACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAG
    CCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAG
    CCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTG
    TTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTG
    GACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAG
    ACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGAC
    TGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCA
    GCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGA
    ACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACG
    GCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAA
    GCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCA
    CAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN 88: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAGCCTGGGCGACTACTACGCCTACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 82 TACAAGGACAACAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACC
    CTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCCAGACTTGGGATATTCTTCCTCATG
    GTCTTGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGTCAGCCTAAGGCTGCCCCCAGCGTGACCCTGT
    TCCCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAG
    GCGCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGC
    AAGCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGG
    TCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8125
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 77
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 89: QAWTDSPTGLV
    VH SEQ ID NO: 79
    VL 90: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQAWTDSPTGLVFGGGTKLTVL
    Heavy chain SEQ ID NO: 81
    Light chain 91: SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQAWTDSPTGLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWK
    ADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding SEQ ID NO: 83
    SEQ ID NO: 79
    PN encoding 92: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 90 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGGCTTGGACTGATTCTCCTACTGGTCTTGT
    GTTTGGCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding SEQ ID NO: 85
    SEQ ID NO: 81
    PN encoding 93: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCGG
    SEQ ID NO: 91 CGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATAA
    GGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGACC
    ATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGGCTTGGACTGATTCTCCTACTGGTCTTGT
    GTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCC
    TCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGA
    CAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCA
    ACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTG
    CCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN SEQ ID NO: 87
    encoding SEQ
    ID NO: 81
    Optimized PN 94: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAGCCTGGGCGACTACTACGCCTACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 91 TACAAGGACAACAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACAGCGGCAACACCGCCACC
    CTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCCAGGCTTGGACTGATTCTCCTACTG
    GTCTTGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGTCAGCCTAAGGCTGCCCCCAGCGTGACCCTGT
    TCCCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAG
    GCGCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGC
    AAGCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGG
    TCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8126
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 62
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 89
    VH SEQ ID NO: 67
    VL SEQ ID NO: 90
    Heavy chain SEQ ID NO: 69
    Light chain SEQ ID NO: 91
    PN encoding SEQ ID NO: 71
    SEQ ID NO: 79
    PN encoding SEQ ID NO: 92
    SEQ ID NO: 90
    PN encoding SEQ ID NO: 73
    SEQ ID NO: 81
    PN encoding SEQ ID NO: 93
    SEQ ID NO: 91
    Optimized PN SEQ ID NO: 75
    encoding SEQ
    ID NO: 81
    Optimized PN SEQ ID NO: 94
    encoding SEQ
    ID NO: 91
    Antibody
    8127
    CDRH1 SEQ ID NO: 61
    CDRH2 95: IIDPTDSYTVYSPSFQG
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 89
    VH 96: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSYTVYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL SEQ ID NO: 90
    Heavy chain 97: EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSYTVYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPP
    CPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS
    VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEW
    ESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain SEQ ID NO: 91
    PN encoding 98: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 96 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATTGATCCTACTGATTCTTATACTGTTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAA
    AAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTT
    ATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGC
    PN encoding SEQ ID NO: 92
    SEQ ID NO: 90
    PN encoding 99: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAGG
    SEQ ID NO: 97 TTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGG
    GCATTATTGATCCTACTGATTCTTATACTGTTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAA
    AAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTT
    ATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGCCTCCACCAAGGGT
    CCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTC
    AAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC
    CCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCA
    CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATC
    TTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTC
    CCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCC
    ACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCG
    GGAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGG
    CAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAA
    GGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGC
    CTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAG
    AACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGG
    ACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACAC
    GCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding SEQ ID NO: 93
    SEQ ID NO: 91
    Optimized PN 100: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAG
    encoding SEQ GGCAGCGGCTACAGCTTCACCAGCTACTACATCGGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGG
    ID NO: 97 ATGGGCATTATTGATCCTACTGATTCTTATACTGTTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATCAGCGCC
    GACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC
    GCCCGGTACATGATGAGGGGCTTCGACCACTGGGGTCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACC
    AAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTG
    CCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCA
    CACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAG
    CCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAG
    CCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTG
    TTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTG
    GACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAG
    ACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGAC
    TGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCA
    GCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGA
    ACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACG
    GCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAA
    GCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCA
    CAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN SEQ ID NO: 94
    encoding SEQ
    ID NO: 91
    Antibody
    8128
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 49
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 101: STWDIEPTYV
    VH SEQ ID NO: 51
    VL 102: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCSTWDIEPTYVFGGGTKLTVL
    Heavy chain SEQ ID NO: 53
    Light chain 103: SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCSTWDIEPTYVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
    SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding SEQ ID NO: 55
    SEQ ID NO: 51
    PN encoding 104: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 102 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCTCTACTTGGGATATTGAGCCTACTTATGTGT
    TTGGCGGCGGCACGAAGTTAACCGTCCTA
    PN encoding SEQ ID NO: 57
    SEQ ID NO: 53
    PN encoding 105: AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 103 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCTCTACTTGGGATATTGAGCCTACTTATGTGT
    TTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC
    CTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA
    GTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAAC
    AACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCC
    AGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN SEQ ID NO: 59
    encoding SEQ
    ID NO: 53
    Optimized PN 106: AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGCTGCAGC
    encoding SEQ GGCGACAATATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATC
    ID NO: 103 TACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCAGCAACTCCGGCAACACCGCCACC
    CTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCTCTACTTGGGATATTGAGCCTACTT
    ATGTGTTCGGCGGAGGGACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCC
    CCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCG
    CCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAG
    CAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTCC
    TACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    8129
    CDRH1 SEQ ID NO: 17
    CDRH2 107: IIDPQDSYTEYSPSFQG
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 108: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPQDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL SEQ ID NO: 24
    Heavy chain 109: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPQDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPC
    PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV
    LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE
    SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain SEQ ID NO: 26
    PN encoding 110: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 108 ATTATTGATCCTCAGGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 28
    SEQ ID NO: 24
    PN encoding 111: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 109 ATTATTGATCCTCAGGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCAT
    CGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGG
    ACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGG
    CTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCA
    GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTG
    ACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCCCCC
    AAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAA
    GACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAG
    GAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAG
    GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGC
    AGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGA
    CCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACA
    ACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAA
    GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAG
    AAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding SEQ ID NO: 30
    SEQ ID NO: 26
    Optimized PN 112: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAG
    encoding SEQ GGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGATG
    ID NO: 109 GGCATCATCGACCCCCAGGACAGCTACACCGAGTACAGCCCCAGCTTCCAGGGCCAGGTGACCATCAGCGCC
    GACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC
    GCCAGATACGAGTACGGCGGCTTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACC
    AAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTG
    CCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCA
    CACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAG
    CCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAG
    CCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTG
    TTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTG
    GACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAG
    ACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGAC
    TGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCA
    GCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGA
    ACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACG
    GCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAA
    GCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCA
    CAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN SEQ ID NO: 32
    encoding SEQ
    ID NO: 26
    Antibody
    8130
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 107
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 101
    VH SEQ ID NO: 108
    VL SEQ ID NO: 102
    Heavy chain SEQ ID NO: 109
    Light chain SEQ ID NO: 103
    PN encoding SEQ ID NO: 110
    SEQ ID
    NO: 108
    PN encoding SEQ ID NO: 104
    SEQ ID
    NO: 102
    PN encoding SEQ ID NO: 111
    SEQ ID
    NO: 109
    PN encoding SEQ ID NO: 105
    SEQ ID
    NO: 103
    Optimized PN SEQ ID NO: 112
    encoding SEQ
    ID NO: 109
    Optimized PN SEQ ID NO: 106
    encoding SEQ
    ID NO: 103
    Antibody
    8131
    CDRH1 SEQ ID NO: 17
    CDRH2 113: IIDPEDSHTEYSPSFQG
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 114: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPEDSHTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL SEQ ID NO: 24
    Heavy chain 115: EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPEDSHTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPC
    PAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV
    LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE
    SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain SEQ ID NO: 26
    PN encoding 116: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 114 ATTATTGATCCTGAGGATTCTCATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 28
    SEQ ID NO: 24
    PN encoding 117: GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 115 ATTATTGATCCTGAGGATTCTCATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCA
    TCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG
    GACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCG
    GCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCC
    AGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
    GACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGACCGTCAGTCTTCCTCTTCCCCC
    CAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGA
    AGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGA
    GGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAA
    GGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGG
    CAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTG
    ACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAAC
    AACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACA
    AGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCA
    GAAGAGCCTCTCCCTGTCTCCGGGTAAA
    PN encoding SEQ ID NO: 30
    SEQ ID NO: 26
    Optimized PN 118: GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGCTGCAAG
    encoding SEQ GGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAGGGCCTGGAGTGGATG
    ID NO: 115 GGCATCATCGACCCCGAGGACAGCCATACCGAGTACAGCCCCAGCTTCCAGGGCCAGGTGACCATCAGCGCC
    GACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC
    GCCAGATACGAGTACGGCGGCTTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACC
    AAGGGCCCCAGCGTGTTCCCCCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTG
    CCTGGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCA
    CACCTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAGCAG
    CCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTGGAG
    CCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAAGCTGCAGGCGGCCCTTCCGTG
    TTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCAGGACCCCCGAGGTGACCTGCGTGGTGGTG
    GACGTGAGCCACGAGGACCCAGAGGTGAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAG
    ACCAAGCCCAGAGAGGAGCAGTACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGAC
    TGGCTGAACGGCAAAGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCA
    GCAAGGCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAGA
    ACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACG
    GCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAA
    GCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCA
    CAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCCGGCAAG
    Optimized PN SEQ ID NO: 32
    encoding SEQ
    ID NO: 26
    Antibody
    8132
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 113
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 101
    VH SEQ ID NO: 114
    VL SEQ ID NO: 102
    Heavy chain SEQ ID NO: 115
    Light chain SEQ ID NO: 103
    PN encoding SEQ ID NO: 116
    SEQ ID
    NO: 114
    PN encoding SEQ ID NO: 104
    SEQ ID
    NO: 102
    PN encoding SEQ ID NO: 117
    SEQ ID
    NO: 115
    PN encoding SEQ ID NO: 105
    SEQ ID
    NO: 103
    Optimized PN SEQ ID NO: 118
    encoding SEQ
    ID NO: 115
    Optimized PN SEQ ID NO: 106
    encoding SEQ
    ID NO: 103
    Antibody
    8091
    CDRH1 SEQ ID NO: 1
    CDRH2 119: NIGPFFGIANYAQKFQG
    CDRH3 SEQ ID NO: 3
    CDRL1 SEQ ID NO: 4
    CDRL2 SEQ ID NO: 5
    CDRL3 120: QTYDDGSTAEV
    VH 121: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGNIGPFFGIANYAQKFQGRVTITAD
    ESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSS
    VL 122: DIELTQPPSVSVAPGQTARISCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTYDDGSTAEVFGGGTKLTVL
    Heavy chain 123: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGNIGPFFGIANYAQKFQGRVTITAD
    ESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCP
    APPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVVSVLTV
    VHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNG
    QPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    Light chain 124: DIELTQPPSVSVAPGQTARISCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTYDDGSTAEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS
    PN encoding 125: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    NO: 121 GGGCAATATCGGTCCGTTTTTTGGCATTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 126: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTATTCCTAATTATTATGTTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATG
    NO: 122 ATGATTCTAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTATGATGATGGTTCTACTGCTGAGG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 127: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    NO: 123 GGGCAATATCGGTCCGTTTTTTGGCATTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCTTCCACCAAGGGC
    CCCAGCGTGTTCCCCCTGGCCCCCTGCAGCAGAAGCACCAGCGAGAGCACAGCCGCCCTGGGCTGCCTGGTG
    AAGGACTACTTCCCCGAGCCCGTGACCGTGAGCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTTC
    CCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGAGCAGCGTGGTGACCGTGCCCAGCAGCAACTTCGGC
    ACCCAGACCTACACCTGCAACGTGGACCACAAGCCCAGCAACACCAAGGTGGACAAGACCGTGGAGCGGAAG
    TGCTGCGTGGAGTGCCCCCCCTGCCCTGCCCCTCCTGTGGCCGGACCCTCCGTGTTCCTGTTCCCCCCCAAG
    CCCAAGGACACCCTGATGATCAGCCGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGAC
    CCCGAGGTGCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCCGGGAGGAA
    CAGTTCAACAGCACCTTCCGGGTGGTGTCCGTGCTGACCGTGGTGCACCAGGACTGGCTGAACGGCAAAGAAT
    ACAAGTGCAAGGTGTCCAACAAGGGCCTGCCTGCCCCCATCGAGAAAACCATCAGCAAGACAAAGGGCCAGCC
    CAGGGAACCCCAGGTGTACACCCTGCCCCCCAGCCGGGAGGAAATGACCAAGAACCAGGTGTCCCTGACCTG
    TCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCCGAGAACAACTA
    CAAGACCACCCCCCCCATGCTGGACAGCGACGGCAGCTTCTTCCTGTACAGCAAGCTGACAGTGGACAAGAGC
    CGGTGGCAGCAGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAG
    AGCCTGAGCCTGTCCCCCGGCAAA
    PN encoding 128: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTATTCCTAATTATTATGTTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATG
    NO: 124 ATGATTCTAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTATGATGATGGTTCTACTGCTGAGG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC
    CTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
    Optimized PN 129: CAGGTGCAGCTGGTGCAGTCCGGCGCCGAGGTGAAGAAGCCCGGCTCCTCCGTGAAGGTGTCCTGCAAG
    encoding SEQ GCCTCCGGCGGCACCTTCTCCTCCTACGCCATCTCCTGGGTGCGGCAGGCCCCCGGCCAGGGCCTGGAGTGG
    ID NO: 123 ATGGGCAACATCGGCCCCTTCTTCGGCATCGCCAACTACGCCCAGAAGTTCCAGGGCCGGGTGACCATCACCG
    CCGACGAGTCCACCTCCACCGCCTACATGGAGCTGTCCTCCCTGCGGTCCGAGGACACCGCCGTGTACTACTG
    CGCCCGGGACACCCCCTACTTCGACTACTGGGGCCAGGGCACCCTGGTGACCGTGTCCTCCGCCTCCACCAA
    GGGCCCCTCCGTGTTCCCCCTGGCCCCCTGCTCCCGGTCCACCTCCGAGTCCACCGCCGCCCTGGGCTGCCT
    GGTGAAGGACTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACTCCGGCGCCCTGACCTCCGGCGTGCACAC
    CTTCCCCGCCGTGCTGCAGTCCTCCGGCCTGTACTCCCTGTCCTCCGTGGTGACCGTGCCCTCCTCCAACTTC
    GGCACCCAGACCTACACCTGCAACGTGGACCACAAGCCCTCCAACACCAAGGTGGACAAGACCGTGGAGCGG
    AAGTGCTGCGTGGAGTGCCCCCCCTGCCCCGCCCCCCCCGTGGCCGGCCCCTCCGTGTTCCTGTTCCCCCCC
    AAGCCCAAGGACACCCTGATGATCTCCCGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGTCCCACGAG
    GACCCCGAGGTGCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCCGGGAG
    GAGCAGTTCAACTCCACCTTCCGGGTGGTGTCCGTGCTGACCGTGGTGCACCAGGACTGGCTGAACGGCAAG
    GAGTACAAGTGCAAGGTGTCCAACAAGGGCCTGCCCGCCCCCATCGAGAAGACCATCTCCAAGACCAAGGGC
    CAGCCCCGGGAGCCCCAGGTGTACACCCTGCCCCCCTCCCGGGAGGAGATGACCAAGAACCAGGTGTCCCTG
    ACCTGCCTGGTGAAGGGCTTCTACCCCTCCGACATCGCCGTGGAGTGGGAGTCCAACGGCCAGCCCGAGAAC
    AACTACAAGACCACCCCCCCCATGCTGGACTCCGACGGCTCCTTCTTCCTGTACTCCAAGCTGACCGTGGACAA
    GTCCCGGTGGCAGCAGGGCAACGTGTTCTCCTGCTCCGTGATGCACGAGGCCCTGCACAACCACTACACCCA
    GAAGTCCCTGTCCCTGTCCCCCGGCAAG
    Optimized PN 130: GACATCGAGCTGACCCAGCCCCCCTCCGTGTCCGTGGCCCCCGGCCAGACCGCCCGGATCTCCTGCTCC
    encoding SEQ GGCGACTCCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCCCGTGCTGGTGATCT
    ID NO: 124 ACGACGACTCCAACCGGCCCTCCGGCATCCCCGAGCGGTTCTCCGGCTCCAACTCCGGCAACACCGCCACCC
    TGACCATCTCCGGCACCCAGGCCGAGGACGAGGCCGACTACTACTGCCAGACCTACGACGACGGCTCCACCG
    CCGAGGTGTTCGGCGGCGGCACCAAGCTGACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGT
    TCCCCCCCAGCAGCGAGGAGCTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAG
    GCGCCGTGACCGTGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGC
    AAGCAGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGG
    TCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTGCAGC
    Antibody
    6525
    CDRH1 131: SYWIS
    CDRH2 132: IIDPDDSKTNYSPSFQG
    CDRH3 133: RSYYPMDY
    CDRL1 134: TGTSSDVVGVYNFVS
    CDRL2 135: YVDNRPS
    CDRL3 136: QSFDGFGIDMV
    VH 137: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWISWVRQMPGKGLEWMGIIDPDDSKTNYSPSFQGQVTISAD
    KSISTAYLQWSSLKASDTAMYYCARRSYYPMDYWGQGTLVTVSS
    VL 138: DIALTQPASVSGSPGQSITISCTGTSSDVVGVYNFVSWYQQHPGKAPKLMIYYVDNRPSGVSNRFSGSKSGNTA
    SLTISGLQAEDEADYYCQSFDGFGIDMVFGGGTKLTVL
    Heavy chain 139: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWISWVRQMPGKGLEWMGIIDPDDSKTNYSPSFQGQVTISAD
    KSISTAYLQWSSLKASDTAMYYCARRSYYPMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDY
    FPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX 
    (X can be C, EF or CEF)
    Light chain 140: DIALTQPASVSGSPGQSITISCTGTSSDVVGVYNFVSWYQQHPGKAPKLMIYYVDNRPSGVSNRFSGSKSGNTA
    SLTISGLQAEDEADYYCQSFDGFGIDMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTV
    AWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 141: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTGGATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 137 GGCATTATCGATCCGGATGATAGCAAGACCAATTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTCGTTCTTATTATCCTATGGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 142: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGTTGGTGTTTATAATTTTGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAA
    NO: 138 CTTATGATTTATTATGTTGATAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACC
    GCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTTTGATGGTTTTGG
    TATTGATATGGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 143: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTGGATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 139 GGCATTATCGATCCGGATGATAGCAAGACCAATTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTCGTTCTTATTATCCTATGGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 144: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGTTGGTGTTTATAATTTTGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAA
    NO: 140 CTTATGATTTATTATGTTGATAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACC
    GCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTTTGATGGTTTTGG
    TATTGATATGGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACG
    CTGTTTCCGCCGAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATC
    CGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCC
    TCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACA
    GAAGCTACAGCTGCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    6756
    CDRH1 145: SYWIA
    CDRH2 146: IIYPGDSDTNYSPSFQG
    CDRH3 147: SKYGSFDY
    CDRL1 148: TGTSSDVGGYNYVS
    CDRL2 149: NVNSRPS
    CDRL3 150: QSYDDGQDNEV
    VH 151: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIAWVRQMPGKGLEWMGIIYPGDSDTNYSPSFQGQVTISAD
    KSISTAYLQWSSLKASDTAMYYCARSKYGSFDYWGQGTLVTVSS
    VL 152: DIALTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYNVNSRPSGVSNRFSGSKSGNTAS
    LTISGLQAEDEADYYCQSYDDGQDNEVFGGGTKLTVL
    Heavy chain 153: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYWIAWVRQMPGKGLEWMGIIYPGDSDTNYSPSFQGQVTISAD
    KSISTAYLQWSSLKASDTAMYYCARSKYGSFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDY
    FPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX 
    (X can be C, EF or CEF)
    Light chain 154: DIALTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYNVNSRPSGVSNRFSGSKSGNTAS
    LTISGLQAEDEADYYCQSYDDGQDNEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTV
    AWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 155: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTGGATTGCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 151 GGCATTATCTATCCGGGTGATAGCGATACCAATTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTCTAAGTATGGTTCTTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 156: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGGTGGTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 152 ATGATTTATAATGTTAATTCTCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATGATGGTCAG
    GATAATGAGGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 157: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTGGATTGCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 153 GGCATTATCTATCCGGGTGATAGCGATACCAATTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTCTAAGTATGGTTCTTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 158: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGGTGGTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 154 ATGATTTATAATGTTAATTCTCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATGATGGTCAG
    GATAATGAGGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGC
    TGTTTCCGCCGAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCC
    GGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCT
    CCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAG
    AAGCTACAGCTGCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    6757
    CDRH1 159: SYAMH
    CDRH2 160: AISSSGSSTYYADSVKG
    CDRH3 161: ESWFLDL
    CDRL1 162: RASQSISNWLA
    CDRL2 163: LASSLQS
    CDRL3 164: QQYYDFSDT
    VH 165: QVQLVESGGGLVQPGGSLRLSCAASGFTFTSYAMHWVRQAPGKGLEWVSAISSSGSSTYYADSVKGRFTISRD
    NSKNTLYLQMNSLRAEDTAVYYCARESWFLDLWGQGTLVTVSS
    VL 166: DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIYLASSLQSGVPSRFSGSGSGTDFTLTI
    SSLQPEDFAVYYCQQYYDFSDTFGQGTKVEIK
    Heavy chain 167: QVQLVESGGGLVQPGGSLRLSCAASGFTFTSYAMHWVRQAPGKGLENVSAISSSGSSTYYADSVKGRFTISRD
    NSKNTLYLQMNSLRAEDTAVYYCARESWFLDLWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
    PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 168: DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIYLASSLQSGVPSRFSGSGSGTDFTLTI
    SSLQPEDFAVYYCQQYYDFSDTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDN
    ALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEX 
    (X can be C or A)
    PN encoding 169: CAGGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACCGGGCGGCAGCCTGCGTCTGAGCTGCGC
    SEQ ID GGCCTCCGGATTTACCTTTACTTCTTATGCTATGCATTGGGTGCGCCAAGCCCCTGGGAAGGGTCTCGAGTGG
    NO: 165 GTGAGCGCTATCTCTTCTTCTGGTAGCTCTACCTATTATGCGGATAGCGTGAAAGGCCGTTTTACCATTTCACGT
    GATAATTCGAAAAACACCCTGTATCTGCAAATGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCGC
    GCGTGAGTCTTGGTTTCTTGATCTTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 170: GATATCCAGATGACCCAGAGCCCGTCTAGCCTGAGCGCGAGCGTGGGTGATCGTGTGACCATTACCTGCA
    SEQ ID GAGCGAGCCAGTCTATTTCTAATTGGCTGGCTTGGTACCAGCAGAAACCAGGTAAAGCACCGAAACTATTAATT
    NO: 166 TATCTTGCTTCTTCTTTGCAAAGCGGGGTCCCGTCCCGTTTTAGCGGCTCTGGATCCGGCACTGATTTTACCCT
    GACCATTAGCAGCCTGCAACCTGAAGACTTTGCGGTTTATTATTGCCAGCAGTATTATGATTTTTCTGATACCTTT
    GGCCAGGGTACGAAAGTTGAAATTAAA
    PN encoding 171: CAGGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACCGGGCGGCAGCCTGCGTCTGAGCTGCGC
    SEQ ID GGCCTCCGGATTTACCTTTACTTCTTATGCTATGCATTGGGTGCGCCAAGCCCCTGGGAAGGGTCTCGAGTGG
    NO: 167 GTGAGCGCTATCTCTTCTTCTGGTAGCTCTACCTATTATGCGGATAGCGTGAAAGGCCGTTTTACCATTTCACGT
    GATAATTCGAAAAACACCCTGTATCTGCAAATGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCGC
    GCGTGAGTCTTGGTTTCTTGATCTTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 172: GATATCCAGATGACCCAGAGCCCGTCTAGCCTGAGCGCGAGCGTGGGTGATCGTGTGACCATTACCTGCA
    SEQ ID GAGCGAGCCAGTCTATTTCTAATTGGCTGGCTTGGTACCAGCAGAAACCAGGTAAAGCACCGAAACTATTAATT
    NO: 168 TATCTTGCTTCTTCTTTGCAAAGCGGGGTCCCGTCCCGTTTTAGCGGCTCTGGATCCGGCACTGATTTTACCCT
    GACCATTAGCAGCCTGCAACCTGAAGACTTTGCGGTTTATTATTGCCAGCAGTATTATGATTTTTCTGATACCTTT
    GGCCAGGGTACGAAAGTTGAAATTAAACGTACGGTGGCTGCTCCGAGCGTGTTTATTTTTCCGCCGAGCGATGA
    ACAACTGAAAAGCGGCACGGCGAGCGTGGTGTGCCTGCTGAACAACTTTTATCCGCGTGAAGCGAAAGTTCAG
    TGGAAAGTAGACAACGCGCTGCAAAGCGGCAACAGCCAGGAAAGCGTGACCGAACAGGATAGCAAAGATAGCA
    CCTATTCTCTGAGCAGCACCCTGACCCTGAGCAAAGCGGATTATGAAAAACATAAAGTGTATGCGTGCGAAGTG
    ACCCATCAAGGTCTGAGCAGCCCGGTGACTAAATCTTTTAATCGTGGCGAGX 
    (X can be TGC or GCC)
    Antibody
    6763
    CDRH1 173: NYGMH
    CDRH2 174: VSYAGSFTNYADSVKG
    CDRH3 175: SWLFGYPDIFDY
    CDRL1 176: TGTSSDVGGYNYVS
    CDRL2 177: DVNNRPS
    CDRL3 178: SSYDKFQTV
    VH 179: QVQLVESGGGLVQPGGSLRLSCAASGFTFSNYGMHWVRQAPGKGLEWVSVSYAGSFTNYADSVKGRFTISRD
    NSKNTLYLQMNSLRAEDTAVYYCARSWLFGYPDIFDYWGQGTLVTVSS
    VL 180: DIALTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVNNRPSGVSNRFSGSKSGNTA
    SLTISGLQAEDEADYYCSSYDKFQTVFGGGTKLTVL
    Heavy chain 181: QVQLVESGGGLVQPGGSLRLSCAASGFTFSNYGMHWVRQAPGKGLEWVSVSYAGSFTNYADSVKGRFTISRD
    NSKNTLYLQMNSLRAEDTAVYYCARSWLFGYPDIFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCL
    VKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 182: DIALTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVNNRPSGVSNRFSGSKSGNTA
    SLTISGLQAEDEADYYCSSYDKFQTVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAW
    KADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 183: CAGGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACCGGGCGGCAGCCTGCGTCTGAGCTGCGC
    SEQ ID GGCCTCCGGATTTACCTTTTCTAATTATGGTATGCATTGGGTGCGCCAAGCCCCTGGGAAGGGTCTCGAGTGG
    NO: 179 GTGAGCGTTTCTTATGCTGGTAGCTTTACCAATTATGCGGATAGCGTGAAAGGCCGTTTTACCATTTCACGTGAT
    AATTCGAAAAACACCCTGTATCTGCAAATGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCGCGCG
    TTCTTGGCTTTTTGGTTATCCTGATATTTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 184: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGGTGGTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 180 ATGATTTATGATGTTAATAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCTCTTCTTATGATAAGTTTCAGA
    CTGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 185: CAGGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACCGGGCGGCAGCCTGCGTCTGAGCTGCGC
    SEQ ID GGCCTCCGGATTTACCTTTTCTAATTATGGTATGCATTGGGTGCGCCAAGCCCCTGGGAAGGGTCTCGAGTGG
    NO: 181 GTGAGCGTTTCTTATGCTGGTAGCTTTACCAATTATGCGGATAGCGTGAAAGGCCGTTTTACCATTTCACGTGAT
    AATTCGAAAAACACCCTGTATCTGCAAATGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCGCGCG
    TTCTTGGCTTTTTGGTTATCCTGATATTTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTC
    GACCAAAGGTCCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGG
    CTGCCTGGTTAAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTG
    CATACCTTTCCGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCA
    GCTTAGGCACTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAA
    CCGAAAAGCX 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 186: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATGTTGGTGGTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 182 ATGATTTATGATGTTAATAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCTCTTCTTATGATAAGTTTCAGA
    CTGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCC
    GCCGAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCC
    GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAA
    AGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACA
    GCTGCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7086
    CDRH1 SEQ ID NO: 1
    CDRH2 SEQ ID NO: 2
    CDRH3 SEQ ID NO: 3
    CDRL1 SEQ ID NO: 4
    CDRL2 SEQ ID NO: 5
    CDRL3 SEQ ID NO: 6
    VH 187: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQGRVTITA
    DESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSS
    VL 188: DIELTQPPSVSVAPGQTARISCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQSFDSSLNAEVFGGGTKLTVL
    Heavy chain 189: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQGRVTITA
    DESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDY
    FPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 190: DIELTQPPSVSVAPGQTARISCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQSFDSSLNAEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 191: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    NO: 187 GGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 192: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTATTCCTAATTATTATGTTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATG
    NO: 188 ATGATTCTAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTTTGATTCTTCTCTTAATGCTGAGGT
    GTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 193: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    NO: 189 GGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 194: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTATTCCTAATTATTATGTTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATG
    NO: 190 ATGATTCTAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTTTGATTCTTCTCTTAATGCTGAGGT
    GTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCC
    GAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody 
    7087
    CDRH1 195: SYYIS
    CDRH2 196: GIIPIFGTANYAQKFQG
    CDRH3 197: GEIWHVHQPYKSGVYGAAY
    CDRL1 198: RASQGISNWLN
    CDRL2 199: GTSSLQS
    CDRL3 200: QQLDSFPAT
    VH 201: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYYISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADE
    STSTAYMELSSLRSEDTAVYYCARGEIWHVHQPYKSGVYGAAYWGQGTLVTVSS
    VL 202: DIQMTQSPSSLSASVGDRVTITCRASQGISNWLNWYQQKPGKAPKLLIYGTSSLQSGVPSRFSGSGSGTDFTLTI
    SSLQPEDFATYYCQQLDSFPATFGQGTKVEIK
    Heavy chain 203: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYYISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADE
    STSTAYMELSSLRSEDTAVYYCARGEIWHVHQPYKSGVYGAAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGT
    AALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVE
    PKSX 
    (X can be C, EF or CEF)
    Light chain 204: DIQMTQSPSSLSASVGDRVTITCRASQGISNWLNWYQQKPGKAPKLLIYGTSSLQSGVPSRFSGSGSGTDFTLTI
    SSLQPEDFATYYCQQLDSFPATFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDN
    ALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEX
    (X can be C or A)
    PN encoding 205: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATTATATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGATG
    NO: 201 GGCGGTATCATTCCGATTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCGG
    ATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCGC
    GCGTGGTGAGATTTGGCATGTTCATCAGCCTTATAAGTCTGGTGTTTATGGTGCTGCTTATTGGGGCCAAGGCA
    CCCTGGTGACGGTTAGCTCA
    PN encoding 206: GATATCCAGATGACCCAGAGCCCGTCTAGCCTGAGCGCGAGCGTGGGTGATCGTGTGACCATTACCTGCA
    SEQ ID GAGCGAGCCAGGGTATTTCTAATTGGCTGAATTGGTACCAGCAGAAACCAGGTAAAGCACCGAAACTATTAATT
    NO: 202 TATGGTACTTCTTCTTTGCAAAGCGGGGTCCCGTCCCGTTTTAGCGGCTCTGGATCCGGCACTGATTTTACCCT
    GACCATTAGCAGCCTGCAACCTGAAGACTTTGCGACTTATTATTGCCAGCAGCTTGATTCTTTTCCTGCTACCTT
    TGGCCAGGGTACGAAAGTTGAAATTAAA
    PN encoding 207: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATTATATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGATG
    NO: 203 GGCGGTATCATTCCGATTTTTGGCACTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCGG
    ATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCGC
    GCGTGGTGAGATTTGGCATGTTCATCAGCCTTATAAGTCTGGTGTTTATGGTGCTGCTTATTGGGGCCAAGGCA
    CCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTCCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCAC
    CAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTAAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAAC
    AGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCCGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGC
    AGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCACTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAA
    CACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 208: GATATCCAGATGACCCAGAGCCCGTCTAGCCTGAGCGCGAGCGTGGGTGATCGTGTGACCATTACCTGCA
    SEQ ID GAGCGAGCCAGGGTATTTCTAATTGGCTGAATTGGTACCAGCAGAAACCAGGTAAAGCACCGAAACTATTAATT
    NO: 204 TATGGTACTTCTTCTTTGCAAAGCGGGGTCCCGTCCCGTTTTAGCGGCTCTGGATCCGGCACTGATTTTACCCT
    GACCATTAGCAGCCTGCAACCTGAAGACTTTGCGACTTATTATTGCCAGCAGCTTGATTCTTTTCCTGCTACCTT
    TGGCCAGGGTACGAAAGTTGAAATTAAACGTACGGTGGCTGCTCCGAGCGTGTTTATTTTTCCGCCGAGCGATG
    AACAACTGAAAAGCGGCACGGCGAGCGTGGTGTGCCTGCTGAACAACTTTTATCCGCGTGAAGCGAAAGTTCA
    GTGGAAAGTAGACAACGCGCTGCAAAGCGGCAACAGCCAGGAAAGCGTGACCGAACAGGATAGCAAAGATAG
    CACCTATTCTCTGAGCAGCACCCTGACCCTGAGCAAAGCGGATTATGAAAAACATAAAGTGTATGCGTGCGAAG
    TGACCCATCAAGGTCTGAGCAGCCCGGTGACTAAATCTTTTAATCGTGGCGAGX 
    (X can be TGC or GCC)
    Antibody
    7091
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 77
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 209: QSWTDSPNTLV
    VH 210: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPSDSHTTYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL 211: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQSWTDSPNTLVFGGGTKLTVL
    Heavy chain 212: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPSDSHTTYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
    PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 213: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQSWTDSPNTLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 214: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 210 GGCATTATCGATCCGTCTGATAGCCATACCACTTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 215: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 211 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTGGACTGATTCTCCTAATACTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 216: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 212 GGCATTATCGATCCGTCTGATAGCCATACCACTTATTCTCCGAGCTTTCAGGGCCAGGTGACCATTAGCGCGGA
    TAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGG
    TCCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGT
    TAAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTT
    CCGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGC
    ACTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAG
    CX 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 217: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 213 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTGGACTGATTCTCCTAATACTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCC
    GAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7092
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 49
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 218: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL 219: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCGTYDIESYVFGGGTKLTVL
    Heavy chain 220: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 221: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCGTYDIESYVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADS
    SPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX
    (X can be CS or A)
    PN encoding 222: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 218 ATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGGACAGGTGACCATTAGCGCGGATAA
    AAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTT
    ATGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 223: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 219 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGGTACTTATGATATTGAGTCTTATGTGTTTG
    GCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 224: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 220 ATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGGACAGGTGACCATTAGCGCGGATAA
    AAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTT
    ATGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTCC
    AAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTAA
    AGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCCG
    GCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCACT
    CAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 225: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 221 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGGTACTTATGATATTGAGTCTTATGTGTTTG
    GCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCCGAGCA
    GCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTGACAGT
    GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAA
    CAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCA
    GGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7093
    CDRH1 SEQ ID NO: 33
    CDRH2 226: HIFSDDDKYYSTSLKT
    CDRH3 SEQ ID NO: 35
    CDRL1 SEQ ID NO: 36
    CDRL2 SEQ ID NO: 37
    CDRL3 SEQ ID NO: 38
    VH 227: QVQLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLAHIFSDDDKYYSTSLKTRLTISKDT
    SKNQVVLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSS
    VL 228: DIALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSKSGNTAS
    LTISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVL
    Heavy chain 229: QVQLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLAHIFSDDDKYYSTSLKTRLTISKDT
    SKNQVVLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 230: DIALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSKSGNTAS
    LTISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWK
    ADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 231: CAGGTGCAATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCT
    SEQ ID TTTCCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAG
    NO: 227 TGGCTGGCTCATATCTTTTCTGATGATGATAAGTATTATAGCACCAGCCTGAAAACGCGTCTGACCATTAGCAAA
    GATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCGC
    GCGTGGTCCTTATGGTTTTGATTCTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 232: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 228 ATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATTCTCAGTCTA
    TTGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 233: CAGGTGCAATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCT
    SEQ ID TTTCCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAG
    NO: 229 TGGCTGGCTCATATCTTTTCTGATGATGATAAGTATTATAGCACCAGCCTGAAAACGCGTCTGACCATTAGCAAA
    GATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCGC
    GCGTGGTCCTTATGGTTTTGATTCTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 234: GATATCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGTGTACGG
    SEQ ID GTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGGAAGGCGCCGAAACTT
    NO: 230 ATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGCGGATCCAAAAGCGGCAACACCGC
    GAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCGGATTATTATTGCCAGTCTTATGATTCTCAGTCTA
    TTGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCC
    GCCGAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCC
    GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAA
    AGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACA
    GCTGCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7094
    CDRH1 235: TSGMSVG
    CDRH2 236: LIDWDEDKSYSTSLKT
    CDRH3 237: YNWYNPPGFDN
    CDRL1 238: SGSSSNIGSNYVS
    CDRL2 239: RNDKRPS
    CDRL3 240: QSADSSSMV
    VH 241: QVQLKESGPALVKPTQTLTLTCTFSGFSLSTSGMSVGWIRQPPGKALEWLALIDWDEDKSYSTSLKTRLTISKDT
    SKNQVVLTMTNMDPVDTATYYCARYNWYNPPGFDNWGQGTLVTVSS
    VL 242: DIVLTQPPSVSGAPGQRVTISCSGSSSNIGSNYVSWYQQLPGTAPKLLIYRNDKRPSGVPDRFSGSKSGTSASL
    AITGLQSEDEADYYCQSADSSSMVFGGGTKLTVL
    Heavy chain 243: QVQLKESGPALVKPTQTLTLTCTFSGFSLSTSGMSVGWIRQPPGKALEWLALIDWDEDKSYSTSLKTRLTISKDT
    SKNQVVLTMTNMDPVDTATYYCARYNWYNPPGFDNWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLV
    KDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain 244: DIVLTQPPSVSGAPGQRVTISCSGSSSNIGSNYVSWYQQLPGTAPKLLIYRNDKRPSGVPDRFSGSKSGTSASL
    AITGLQSEDEADYYCQSADSSSMVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWK
    ADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding 245: CAGGTGCAATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCT
    SEQ ID TTTCCGGATTTAGCCTGTCTACTTCTGGTATGTCTGTGGGTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAG
    NO: 241 TGGCTGGCTCTTATCGATTGGGATGAGGATAAGTCTTATAGCACCAGCCTGAAAACGCGTCTGACCATTAGCAA
    AGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCG
    CGCGTTATAATTGGTATAATCCTCCTGGTTTTGATAATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding 246: GATATCGTGCTGACCCAGCCGCCTTCAGTGAGTGGCGCACCAGGTCAGCGTGTGACCATCTCGTGTAGCG
    SEQ ID GCAGCAGCAGCAACATTGGTTCTAATTATGTGTCTTGGTACCAGCAGTTGCCCGGGACGGCGCCGAAACTTCT
    NO: 242 GATTTATCGTAATGATAAGCGTCCCTCAGGCGTGCCGGATCGTTTTAGCGGATCCAAAAGCGGCACCAGCGCG
    AGCCTTGCGATTACGGGCCTGCAAAGCGAAGACGAAGCGGATTATTATTGCCAGTCTGCTGATTCTTCTTCTAT
    GGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding 247: CAGGTGCAATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCTGTACCT
    SEQ ID TTTCCGGATTTAGCCTGTCTACTTCTGGTATGTCTGTGGGTTGGATTCGCCAGCCGCCTGGGAAAGCCCTCGAG
    NO: 243 TGGCTGGCTCTTATCGATTGGGATGAGGATAAGTCTTATAGCACCAGCCTGAAAACGCGTCTGACCATTAGCAA
    AGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATGGACCCGGTGGATACGGCCACCTATTATTGCG
    CGCGTTATAATTGGTATAATCCTCCTGGTTTTGATAATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCG
    TCGACCAAAGGTCCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTG
    GGCTGCCTGGTTAAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGC
    GTGCATACCTTTCCGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCA
    GCAGCTTAGGCACTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTG
    GAACCGAAAAGCX 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding 248: GATATCGTGCTGACCCAGCCGCCTTCAGTGAGTGGCGCACCAGGTCAGCGTGTGACCATCTCGTGTAGCG
    SEQ ID GCAGCAGCAGCAACATTGGTTCTAATTATGTGTCTTGGTACCAGCAGTTGCCCGGGACGGCGCCGAAACTTCT
    NO: 244 GATTTATCGTAATGATAAGCGTCCCTCAGGCGTGCCGGATCGTTTTAGCGGATCCAAAAGCGGCACCAGCGCG
    AGCCTTGCGATTACGGGCCTGCAAAGCGAAGACGAAGCGGATTATTATTGCCAGTCTGCTGATTCTTCTTCTAT
    GGTGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCG
    CCGAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCG
    TGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA
    GCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAG
    CTGCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7821
    CDRH1 SEQ ID NO: 1
    CDRH2 SEQ ID NO: 119
    CDRH3 SEQ ID NO: 3
    CDRL1 SEQ ID NO: 4
    CDRL2 SEQ ID NO: 5
    CDRL3 SEQ ID NO: 6
    VH SEQ ID NO: 121
    VL SEQ ID NO: 188
    Heavy chain 249: QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGNIGPFFGIANYAQKFQGRVTITAD
    ESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFP
    EPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 190
    PN encoding SEQ ID NO: 125
    SEQ ID
    NO: 121
    PN encoding SEQ ID NO: 192
    SEQ ID
    NO: 188
    PN encoding 250: CAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCTGCAAAG
    SEQ ID CCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAGGGTCTCGAGTGGAT
    NO: 249 GGGCAATATCGGTCCGTTTTTTGGCATTGCGAATTACGCGCAGAAGTTTCAGGGCCGGGTGACCATTACCGCG
    GATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTGCGTAGCGAAGATACGGCCGTGTATTATTGCG
    CGCGTGATACTCCTTATTTTGATTATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGT
    CCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTT
    AAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTC
    CGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCA
    CTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGC
    X (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding SEQ ID NO: 194
    SEQ ID
    NO: 190
    Antibody
    7865
    CDRH1 SEQ ID NO: 1
    CDRH2 SEQ ID NO: 2
    CDRH3 SEQ ID NO: 3
    CDRL1 SEQ ID NO: 4
    CDRL2 SEQ ID NO: 5
    CDRL3 SEQ ID NO: 120
    VH SEQ ID NO: 187
    VL SEQ ID NO: 122
    Heavy chain SEQ ID NO: 189
    Light chain 251: DIELTQPPSVSVAPGQTARISCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTYDDGSTAEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX
    (X can be CS or A)
    PN encoding SEQ ID NO: 191
    SEQ ID
    NO: 187
    PN encoding SEQ ID NO: 126
    SEQ ID
    NO: 122
    PN encoding SEQ ID NO: 193
    SEQ ID
    NO: 189
    PN encoding 252: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTATTCCTAATTATTATGTTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATG
    NO: 251 ATGATTCTAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTATGATGATGGTTCTACTGCTGAGG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCC
    GAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7829
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 62
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 209
    VH 253: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL SEQ ID NO: 211
    Heavy chain 254: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
    PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 213
    PN encoding 255: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 253 GGCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGAT
    AAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 215
    SEQ ID
    NO: 211
    PN encoding 256: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 254 GGCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGAT
    AAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGC
    GTTATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGG
    TCCAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGT
    TAAAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTT
    CCGGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGC
    ACTCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAG
    CX 
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding SEQ ID NO: 217
    SEQ ID
    NO: 213
    Antibody
    7830
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 95
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 209
    VH 257: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSYTVYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
    VL SEQ ID NO: 211
    Heavy chain 258: QVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSYTVYSPSFQGQVTISADK
    SISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
    PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 213
    PN encoding 259: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 257 GGCATTATTGATCCTACTGATTCTTATACTGTTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATA
    AAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGT
    TATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 215
    SEQ ID
    NO: 211
    PN encoding 260: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATG
    NO: 258 GGCATTATTGATCCTACTGATTCTTATACTGTTTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATA
    AAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGT
    TATATGATGCGTGGTTTTGATCATTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTC
    CAAGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTA
    AAGATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCC
    GGCGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCAC
    TCAGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX
    (X can be TGC, GAATTC or TGCGAATTC)
    PN encoding SEQ ID NO: 217
    SEQ ID
    NO: 213
    Antibody
    7871
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 77
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 66
    VH SEQ ID NO: 210
    VL 261: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDTGESGVFGGGTKLTVL
    Heavy chain SEQ ID NO: 212
    Light chain 262: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDTGESGVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX
    (X can be CS or A)
    PN encoding SEQ ID NO: 214
    SEQ ID
    NO: 210
    PN encoding 263: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 261 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATACTGGTGAGTCTGGTGTG
    TTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding SEQ ID NO: 216
    SEQ ID
    NO: 212
    PN encoding 264: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 262 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATACTGGTGAGTCTGGTGTG
    TTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCCGA
    GCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTGAC
    AGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAA
    CAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC
    CAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7872
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 77
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 78
    VH SEQ ID NO: 210
    VL 265: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDILPHGLVFGGGTKLTVL
    Heavy chain SEQ ID NO: 212
    Light chain 266: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQTWDILPHGLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKA
    DSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding SEQ ID NO: 214
    SEQ ID
    NO: 210
    PN encoding 267: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 265 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATATTCTTCCTCATGGTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding SEQ ID NO: 216
    SEQ ID
    NO: 212
    PN encoding 268: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 266 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGACTTGGGATATTCTTCCTCATGGTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCC
    GAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7873
    CDRH1 SEQ ID NO: 61
    CDRH2 SEQ ID NO: 77
    CDRH3 SEQ ID NO: 63
    CDRL1 SEQ ID NO: 64
    CDRL2 SEQ ID NO: 65
    CDRL3 SEQ ID NO: 89
    VH SEQ ID NO: 210
    VL 269: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQAWTDSPTGLVFGGGTKLTVL
    Heavy chain SEQ ID NO: 212
    Light chain 270: DIELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCQAWTDSPTGLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWK
    ADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX
    (X can be CS or A)
    PN encoding SEQ ID NO: 214
    SEQ ID
    NO: 210
    PN encoding 271: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 269 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGGCTTGGACTGATTCTCCTACTGGTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding SEQ ID NO: 216
    SEQ ID
    NO: 212
    PN encoding 272: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 270 AGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCCAGGCTTGGACTGATTCTCCTACTGGTCTTG
    TGTTTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCC
    GAGCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTG
    ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGC
    AACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT
    GCCAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7832
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 18
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 273: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL SEQ ID NO: 219
    Heavy chain 274: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 221
    PN encoding 275: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 273 ATTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 223
    SEQ ID
    NO: 219
    PN encoding 276: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 274 ATTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTCCAA
    GCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTAAAG
    ATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCCGGC
    GGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCACTCA
    GACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX
    (X can be TGC, GAATTC or TGCGAATTC)
    Antibody
    7909
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 107
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 277: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPQDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL SEQ ID NO: 219
    Heavy chain 278: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPQDSYTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 221
    PN encoding 279: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 277 ATTATTGATCCTCAGGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 223
    SEQ ID
    NO: 219
    PN encoding 280: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 278 ATTATTGATCCTCAGGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTCACCATTAGCGCGGATAAAA
    GCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTAT
    GAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTCCAA
    GCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTAAAG
    ATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCCGGC
    GGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCACTCA
    GACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX
    (X can be TGC, GAATTC or TGCGAATTC)
    Antibody
    7910
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 113
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 22
    VH 281: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPEDSHTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
    VL SEQ ID NO: 219
    Heavy chain 282: QVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPEDSHTEYSPSFQGQVTISADKS
    ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPE
    PVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX
    (X can be C, EF or CEF)
    Light chain SEQ ID NO: 221
    PN encoding 283: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 281 ATTATTGATCCTGAGGATTCTCATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA
    PN encoding SEQ ID NO: 223
    SEQ ID
    NO: 219
    PN encoding 284: CAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCTGCAAAG
    SEQ ID GTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGTCTCGAGTGGATGGGC
    NO: 282 ATTATTGATCCTGAGGATTCTCATACTGAGTATTCTCCTTCTTTTCAGGGTCAGGTGACCATTAGCGCGGATAAA
    AGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGCGAGCGATACGGCCATGTATTATTGCGCGCGTTA
    TGAGTATGGTGGTTTTGATATTTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCAGCGTCGACCAAAGGTCCA
    AGCGTGTTTCCGCTGGCTCCGAGCAGCAAAAGCACCAGCGGCGGCACGGCTGCCCTGGGCTGCCTGGTTAAA
    GATTATTTCCCGGAACCAGTCACCGTGAGCTGGAACAGCGGGGCGCTGACCAGCGGCGTGCATACCTTTCCGG
    CGGTGCTGCAAAGCAGCGGCCTGTATAGCCTGAGCAGCGTTGTGACCGTGCCGAGCAGCAGCTTAGGCACTC
    AGACCTATATTTGCAACGTGAACCATAAACCGAGCAACACCAAAGTGGATAAAAAAGTGGAACCGAAAAGCX
    (X can be TGC, GAATTC or TGCGAATTC)
    Antibody
    7876
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 49
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 50
    VH SEQ ID NO: 218
    VL 285: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCATWGSEDQVFGGGTKLTVL
    Heavy chain SEQ ID NO: 220
    Light chain 286: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCATWGSEDQVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
    SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX
    (X can be CS or A)
    PN encoding SEQ ID NO: 222
    SEQ ID
    NO: 218
    PN encoding 287: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 285 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGCTACTTGGGGTTCTGAGGATCAGGTGTTT
    GGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding SEQ ID NO: 224
    SEQ ID
    NO: 220
    PN encoding 288: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 286 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCGCTACTTGGGGTTCTGAGGATCAGGTGTTT
    GGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCCGAGC
    AGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTGACAG
    TGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACA
    ACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCA
    GGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Antibody
    7878
    CDRH1 SEQ ID NO: 17
    CDRH2 SEQ ID NO: 49
    CDRH3 SEQ ID NO: 19
    CDRL1 SEQ ID NO: 20
    CDRL2 SEQ ID NO: 21
    CDRL3 SEQ ID NO: 101
    VH SEQ ID NO: 218
    VL 289: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCSTWDIEPTYVFGGGTKLTVL
    Heavy chain SEQ ID NO: 220
    Light chain 290: DIELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSGNTATLTIS
    GTQAEDEADYYCSTWDIEPTYVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKAD
    SSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEX 
    (X can be CS or A)
    PN encoding SEQ ID NO: 222
    SEQ ID
    NO: 218
    PN encoding 291: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 289 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCTCTACTTGGGATATTGAGCCTACTTATGTGT
    TTGGCGGCGGCACGAAGTTAACCGTTCTT
    PN encoding SEQ ID NO: 224
    SEQ ID
    NO: 220
    PN encoding 292: GATATCGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGTGTAGCG
    SEQ ID GCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCAGTTCTTGTGATTTATA
    NO: 290 AGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAACAGCGGCAACACCGCGACCCTGAC
    CATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATTGCTCTACTTGGGATATTGAGCCTACTTATGTGT
    TTGGCGGCGGCACGAAGTTAACCGTTCTTGGCCAGCCGAAAGCCGCACCGAGTGTGACGCTGTTTCCGCCGA
    GCAGCGAAGAATTGCAGGCGAACAAAGCGACCCTGGTGTGCCTGATTAGCGACTTTTATCCGGGAGCCGTGAC
    AGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAA
    CAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC
    CAGGTCACGCATGAGGGGAGCACCGTGGAAAAAACCGTTGCGCCGACTGAGX 
    (X can be TGCAGC or GCC)
    Human (Homo 296: MGLLGILCFLIFLGKTWGQEQTYVISAPKIFRVGASENIVIQVYGYTEAFDATISIKSYPDKKFSYSSGHVHLSSENK
    sapiens) C5 FQNSAILTIQPKQLPGGQNPVSYVYLEVVSKHFSKSKRMPITYDNGFLFIHTDKPVYTPDQSVKVRVYSLNDDLKPAK
    RETVLTFIDPEGSEVDMVEEIDHIGIISFPDFKIPSNPRYGMWTIKAKYKEDFSTTGTAYFEVKEYVLPHFSVSIEPEYNF
    IGYKNFKNFEITIKARYFYNKVVTEADVYITFGIREDLKDDQKEMMQTAMQNTMLINGIAQVTFDSETAVKELSYYSLED
    LNNKYLYIAVTVIESTGGFSEEAEIPGIKYVLSPYKLNLVATPLFLKPGIPYPIKVQVKDSLDQLVGGVPVTLNAQTIDVN
    QETSDLDPSKSVTRVDDGVASFVLNLPSGVTVLEFNVKTDAPDLPEENQAREGYRAIAYSSLSQSYLYIDWTDNHKA
    LLVGEHLNIIVTPKSPYIDKITHYNYLILSKGKIIHFGTREKFSDASYQSINIPVTQNMVPSSRLLVYYIVTGEQTAELVSD
    SVWLNIEEKCGNQLQVHLSPDADAYSPGQTVSLNMATGMDSWVALAAVDSAVYGVQRGAKKPLERVFQFLEKSDL
    GCGAGGGLNNANVFHLAGLTFLTNANADDSQENDEPCKEILRPRRTLQKKIEEIAAKYKHSVVKKCCYDGACVNNDE
    TCEQRAARISLGPRCIKAFTECCVVASQLRANISHKDMQLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQL
    QFALPDSLTTWEIQGVGISNTGICVADTVKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGMQFCVKMSAVE
    GICTSESPVIDHQGTKSSKCVRQKVEGSSSHLVTFTVLPLEIGLHNINFSLETWFGKEILVKTLRVVPEGVKRESYSGV
    TLDPRGIYGTISRRKEFPYRIPLDLVPKTEIKRILSVKGLLVGEILSAVLSQEGINILTHLPKGSAEAELMSVVPVFYVFHY
    LETGNHWNIFHSDPLIEKQKLKKKLKEGMLSIMSYRNADYSYSVWKGGSASTWLTAFALRVLGQVNKYVEQNQNSIC
    NSLLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEARENSLYLTAFTVIGIRKAFDICPLVKIDTALIKADNFLLENTLPA
    QSTFTLAISAYALSLGDKTHPQFRSIVSALKREALVKGNPPIYRFWKDNLQHKDSSVPNTGTARMVETTAYALLTSLNL
    KDINYVNPVIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLSMDIDVSYKHKGALHNYKMTDKNFLGRPVE
    VLLNDDLIVSTGFGSGLATVHVTTVVHKTSTSEEVCSFYLKIDTQDIEASHYRGYGNSDYKRIVACASYKPSREESSSG
    SSHAVMDISLPTGISANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFTVYEY
    HRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTACKPEIAYAYKVSITSITVENVFVKY
    KATLLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSC
    QAFLANLDEFAEDIFLNGC
    Cynomolgus 297: MGLLGILCFLIFLGKTWGQEQTYVISAPKIFRVGASENIVIQVYGYTEAFDATISIKSYPDKKFSYSSGHVHLSSENK
    Macaque FQNSAVLTIQPKQLPGGQNQVSYVYLEVVSKHFSKSKKIPITYDNGFLFIHTDKPVYTPDQSVKVRVYSLNDDLKPAK
    (Macaca RETVLTFIDPEGSEIDMVEEIDHIGIISFPDFKIPSNPRYGMWTIQAKYKEDFSTTGTAFFEVKEYVLPHFSVSVEPESNF
    fascicularis) IGYKNFKNFEITIKARYFYNKVVTEADVYITFGIREDLKDDQKEMMQTAMQNTMLINGIAQVTFDSETAVKELSYYSLED
    C5 LNNKYLYIAVTVIESTGGFSEEAEIPGIKYVLSPYKLNLVATPLFLKPGIPYSIKVQVKDALDQLVGGVPVTLNAQTIDVN
    QETSDLEPRKSVTRVDDGVASFVVNLPSGVTVLEFNVKTDAPDLPDENQAREGYRAIAYSSLSQSYLYIDWTDNHKA
    LLVGEYLNIIVTPKSPYIDKITHYNYLILSKGKIIHFGTREKLSDASYQSINIPVTQNMVPSSRLLVYYIVTGEQTAELVSDS
    VWLNIEEKCGNQLQVHLSPDADTYSPGQTVSLNMVTGMDSWVALTAVDSAVYGVQRRAKKPLERVFQFLEKSDLG
    CGAGGGLNNANVFHLAGLTFLTNANADDSQENDEPCKEIIRPRRMLQEKIEEIAAKYKHLVVKKCCYDGVRINHDETC
    EQRAARISVGPRCVKAFTECCVVASQLRANNSHKDLQLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQLQ
    FALPDSVTTWEIQGVGISNSGICVADTIKAKVFKDVFLEMNIPYSVVRGEQVQLKGTVYNYRTSGMQFCVKMSAVEGI
    CTSESPVIDHQGTKSSKCVRQKVEGSSNHLVTFTVLPLEIGLQNINFSLETSFGKEILVKSLRVVPEGVKRESYSGITLD
    PRGIYGTISRRKEFPYRIPLDLVPKTEIKRILSVKGLLVGEILSAVLSREGINILTHLPKGSAEAELMSVVPVFYVFHYLET
    GNHWNIFHSDPLIEKRNLEKKLKEGMVSIMSYRNADYSYSVWKGGSASTWLTAFALRVLGQVHKYVEQNQNSICNS
    LLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEARENSLYLTAFTVIGIRKAFDICPLVKINTALIKADTFLLENTLPAQS
    TFTLAISAYALSLGDKTHPQFRSIVSALKREALVKGNPPIYRFWKDSLQHKDSSVPNTGTARMVETTAYALLTSLNLKD
    INYVNPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLNMDIDVAYKHKGPLHNYKMTDKNFLGRPVEVLL
    NDDLVVSTGFGSGLATVHVTTVVHKTSTSEEVCSFYLKIDTQDIEASHYRGYGNSDYKRIVACASYKPSKEESSSGSS
    HAVMDISLPTGINANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFTVYEYHR
    PDKQCTMFYSTSNIKIQKVCEGATCKCIEADCGQMQKELDLTISAETRKQTACNPEIAYAYKVIITSITTENVFVKYKAT
    LLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFTFRYIYPLDSLTWIEYWPRDTTCSSCQAF
    LANLDEFAEDIFLNGC
  • Other antibodies of the invention include those where the amino acids or nucleic acids encoding the amino acids have been mutated, yet have at least 60, 70, 80, 90 or 95 percent identity to the sequences described in Table 1. In some embodiments, it include mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the variable regions when compared with the variable regions depicted in the sequence described in Table 1, while retaining substantially the same therapeutic activity.
  • Since each of these antibodies can bind to C5, the VH, VL, full length light chain, and full length heavy chain sequences (amino acid sequences and the nucleotide sequences encoding the amino acid sequences) can be “mixed and matched” to create other C5-binding antibodies of the invention. Such “mixed and matched” C5-binding antibodies can be tested using the binding assays known in the art (e.g., ELISAs, and other assays described in the Example section). When these chains are mixed and matched, a VH sequence from a particular VH/VL pairing should be replaced with a structurally similar VH sequence. Likewise a full length heavy chain sequence from a particular full length heavy chain/full length light chain pairing should be replaced with a structurally similar full length heavy chain sequence. Likewise, a VL sequence from a particular VH/VL pairing should be replaced with a structurally similar VL sequence. Likewise a full length light chain sequence from a particular full length heavy chain/full length light chain pairing should be replaced with a structurally similar full length light chain sequence. Accordingly, in one aspect, the invention provides an isolated monoclonal antibody or antigen binding region thereof having: a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, and 281; and a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, and 289; wherein the antibody specifically binds to C5 (e.g., human and/or cynomologus C5).
  • In another aspect, the invention provides (i) an isolated monoclonal antibody having: a full length heavy chain comprising an amino acid sequence that has been optimized for expression in the cell of a mammalian selected from the group consisting of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282; and a full length light chain comprising an amino acid sequence that has been optimized for expression in the cell of a mammalian selected from the group consisting of SEQ ID NOs: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290; or (ii) a functional protein comprising an antigen binding portion thereof.
  • In another aspect, the present invention provides C5-binding antibodies that comprise the heavy chain and light chain CDR1s, CDR2s and CDR3s as described in Table 1, or combinations thereof. The amino acid sequences of the VH CDR1s of the antibodies are shown in SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235. The amino acid sequences of the VH CDR2s of the antibodies and are shown in SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236. The amino acid sequences of the VH CDR3s of the antibodies are shown in SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237. The amino acid sequences of the VL CDR1s of the antibodies are shown in SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238. The amino acid sequences of the VL CDR2s of the antibodies are shown in SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239. The amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240. The CDR regions are delineated using the Kabat system (Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242).
  • Given that each of these antibodies can bind to C5 and that antigen-binding specificity is provided primarily by the CDR1, 2 and 3 regions, the VH CDR1, 2 and 3 sequences and VL CDR1, 2 and 3 sequences can be “mixed and matched” (i.e., CDRs from different antibodies can be mixed and match, although each antibody must contain a VH CDR1, 2 and 3 and a VL CDR1, 2 and 3 to create other C5-binding binding molecules of the invention. Such “mixed and matched” C5-binding antibodies can be tested using the binding assays known in the art and those described in the Examples (e.g., ELISAs). When VH CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VH sequence should be replaced with a structurally similar CDR sequence(s). Likewise, when VL CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VL sequence should be replaced with a structurally similar CDR sequence(s). It will be readily apparent to the ordinarily skilled artisan that novel VH and VL sequences can be created by substituting one or more VH and/or VL CDR region sequences with structurally similar sequences from the CDR sequences shown herein for monoclonal antibodies of the present invention.
  • Accordingly, the present invention provides an isolated monoclonal antibody or antigen binding region thereof comprising a heavy chain variable region CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a heavy chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; a heavy chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237; a light chain variable region CDR1 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a light chain variable region CDR2 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a light chain variable region CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240; wherein the antibody specifically binds C5.
  • In a specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO:1; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 18; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 33; a heavy chain variable region CDR2 of SEQ ID NO: 34; a heavy chain variable region CDR3 of SEQ ID NO: 35; a light chain variable region CDR1 of SEQ ID NO: 36; a light chain variable region CDR2 of SEQ ID NO: 37; and a light chain variable region CDR3 of SEQ ID NO: 38. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 50.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 66. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 78.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 95; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 113; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 113; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 101.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 119; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 120. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 131; a heavy chain variable region CDR2 of SEQ ID NO: 132; a heavy chain variable region CDR3 of SEQ ID NO: 133; a light chain variable region CDR1 of SEQ ID NO: 134; a light chain variable region CDR2 of SEQ ID NO: 135; and a light chain variable region CDR3 of SEQ ID NO: 136.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 145; a heavy chain variable region CDR2 of SEQ ID NO: 146; a heavy chain variable region CDR3 of SEQ ID NO: 147; a light chain variable region CDR1 of SEQ ID NO: 148; a light chain variable region CDR2 of SEQ ID NO: 149; and a light chain variable region CDR3 of SEQ ID NO: 150. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 159; a heavy chain variable region CDR2 of SEQ ID NO: 160; a heavy chain variable region CDR3 of SEQ ID NO: 161; a light chain variable region CDR1 of SEQ ID NO: 162; a light chain variable region CDR2 of SEQ ID NO: 163; and a light chain variable region CDR3 of SEQ ID NO: 164.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 173; a heavy chain variable region CDR2 of SEQ ID NO: 174; a heavy chain variable region CDR3 of SEQ ID NO: 175; a light chain variable region CDR1 of SEQ ID NO: 176; a light chain variable region CDR2 of SEQ ID NO: 177; and a light chain variable region CDR3 of SEQ ID NO: 178. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 195; a heavy chain variable region CDR2 of SEQ ID NO: 196; a heavy chain variable region CDR3 of SEQ ID NO: 197; a light chain variable region CDR1 of SEQ ID NO: 198; a light chain variable region CDR2 of SEQ ID NO: 199; and a light chain variable region CDR3 of SEQ ID NO: 200.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 49; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 33; a heavy chain variable region CDR2 of SEQ ID NO: 226; a heavy chain variable region CDR3 of SEQ ID NO: 35; a light chain variable region CDR1 of SEQ ID NO: 36; a light chain variable region CDR2 of SEQ ID NO: 37; and a light chain variable region CDR3 of SEQ ID NO: 38. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 235; a heavy chain variable region CDR2 of SEQ ID NO: 236; a heavy chain variable region CDR3 of SEQ ID NO: 237; a light chain variable region CDR1 of SEQ ID NO: 238; a light chain variable region CDR2 of SEQ ID NO: 239; and a light chain variable region CDR3 of SEQ ID NO: 240.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 119; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 1; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 120.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 62; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 95; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 209.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 66. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 78.
  • In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 61; a heavy chain variable region CDR2 of SEQ ID NO: 77; a heavy chain variable region CDR3 of SEQ ID NO: 63; a light chain variable region CDR1 of SEQ ID NO: 64; a light chain variable region CDR2 of SEQ ID NO: 65; and a light chain variable region CDR3 of SEQ ID NO: 89. In another specific embodiment, an antibody that specifically binds to C5 comprising a heavy chain variable region CDR1 of SEQ ID NO: 17; a heavy chain variable region CDR2 of SEQ ID NO: 107; a heavy chain variable region CDR3 of SEQ ID NO: 19; a light chain variable region CDR1 of SEQ ID NO: 20; a light chain variable region CDR2 of SEQ ID NO: 21; and a light chain variable region CDR3 of SEQ ID NO: 22.
  • In certain embodiments, an antibody that specifically binds to C5 is an antibody that is described in Table 1.
  • As used herein, a human antibody comprises heavy or light chain variable regions or full length heavy or light chains that are “the product of” or “derived from” a particular germline sequence if the variable regions or full length chains of the antibody are obtained from a system that uses human germline immunoglobulin genes. Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest. A human antibody that is “the product of” or “derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is “the product of” or “derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally occurring somatic mutations or intentional introduction of site-directed mutations. However, in the VH or VL framework regions, a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a human antibody may be at least 60%, 70%, 80%, 90%, or at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene. Typically, a recombinant human antibody will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene in the VH or VL framework regions. In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene.
    • Homologous Antibodies
  • In yet another embodiment, the present invention provides an antibody or an antigen-binding fragment thereof comprising amino acid sequences that are homologous to the sequences described in Table 1, and said antibody binds to a C5 protein (e.g., human and/or cynomologus C5), and retains the desired functional properties of those antibodies described in Table 1.
  • For example, the invention provides an isolated monoclonal antibody (or a functional antigen binding fragment thereof) comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281; the light chain variable region comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289; the antibody specifically binds to C5 (e.g., human and/or cynomologus C5), and the antibody can inhibit red blood cell lysis in a hemolytic assay. In a specific example, such antibodies have an IC50 value in a hemolytic assay of 20-200 pM when using human C5-depleted serum that is reconstituted with 100 pM human C5.
  • In other embodiments, the VH and/or VL amino acid sequences may be 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1. In other embodiments, the VH and/or VL amino acid sequences may be identical except an amino acid substitution in no more than 1, 2, 3, 4 or 5 amino acid position. An antibody having VH and VL regions having high (i.e., 80% or greater) identity to the VH and VL regions of those described in Table 1 can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281; and 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289 respectively, followed by testing of the encoded altered antibody for retained function using the functional assays described herein.
  • In other embodiments, the full length heavy chain and/or full length light chain amino acid sequences may be 50% 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1. An antibody having a full length heavy chain and full length light chain having high (i.e., 80% or greater) identity to the full length heavy chains of any of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282 and full length light chains of any of SEQ ID NOs 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290 respectively, can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding such polypeptides respectively, followed by testing of the encoded altered antibody for retained function using the functional assays described herein.
  • In other embodiments, the full length heavy chain and/or full length light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth above.
  • In other embodiments, the variable regions of heavy chain and/or light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth above
  • As used herein, the percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity equals number of identical positions/total number of positions×100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as described in the non-limiting examples below.
  • Additionally or alternatively, the protein sequences of the present invention can further be used as a “query sequence” to perform a search against public databases to, for example, identify related sequences. For example, such searches can be performed using the BLAST program (version 2.0) of Altschul, et al., 1990 J. Mol. Biol. 215:403-10.
  • Antibodies with Conservative Modifications
  • In certain embodiments, an antibody of the invention has a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein one or more of these CDR sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the C5-binding antibodies of the invention. Accordingly, the invention provides an isolated monoclonal antibody, or a functional antigen binding fragment thereof, consisting of a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein: the heavy chain variable region CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235, and conservative modifications thereof; the heavy chain variable region CDR2 amino acid sequences are selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, and conservative modifications thereof; the heavy chain variable region CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237, and conservative modifications thereof; the light chain variable regions CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238, and conservative modifications thereof; the light chain variable regions CDR2 amino acid sequences are selected from the group consisting of SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239, and conservative modifications thereof; the light chain variable regions of CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240, and conservative modifications thereof; the antibody or the antigen-binding fragment thereof specifically binds to C5, and inhibits red blood cell lysis in a hemolytic assay as described herein.
  • In other embodiments, an antibody of the invention optimized for expression in a mammalian cell has a full length heavy chain sequence and a full length light chain sequence, wherein one or more of these sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the C5-binding antibodies of the invention. Accordingly, the invention provides an isolated monoclonal antibody optimized for expression in a mammalian cell consisting of a full length heavy chain and a full length light chain wherein: the full length heavy chain has amino acid sequences selected from the group of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282, and conservative modifications thereof; and the full length light chain has amino acid sequences selected from the group of SEQ ID NOs: 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290, and conservative modifications thereof; the antibody specifically binds to C5 (e.g., human and/or cynomologus C5); and the antibody inhibits red blood cell lysis in a hemolytic assay as described herein. In a specific embodiment, such antibodies have an IC50 value in a hemolytic assay of 20-200 pM when using human C5-depleted serum that is reconstituted with 100 pM human C5.
  • Antibodies that Bind to the Same Epitope
  • The present invention provides antibodies that bind to the same epitope as do the C5-binding antibodies described in Table 1. Additional antibodies can therefore be identified based on their ability to cross-compete (e.g., to competitively inhibit the binding of, in a statistically significant manner) with other antibodies of the invention in C5 binding assays. The ability of a test antibody to inhibit the binding of antibodies of the present invention to a C5 protein (e.g., human and/or cynomolgus C5) demonstrates that the test antibody can compete with that antibody for binding to C5; such an antibody may, according to non-limiting theory, bind to the same or a related (e.g., a structurally similar or spatially proximal) epitope on the C5 protein as the antibody with which it competes. In a certain embodiment, the antibody that binds to the same epitope on C5 as the antibodies of the present invention is a human monoclonal antibody. Such human monoclonal antibodies can be prepared and isolated as described herein.
    • Engineered and Modified Antibodies
  • An antibody of the invention further can be prepared using an antibody having one or more of the VH and/or VL sequences shown herein as starting material to engineer a modified antibody, which modified antibody may have altered properties from the starting antibody. An antibody can be engineered by modifying one or more residues within one or both variable regions (i.e., VH and/or VL), for example within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be engineered by modifying residues within the constant region(s), for example to alter the effector function(s) of the antibody.
  • One type of variable region engineering that can be performed is CDR grafting. Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann, L. et al., 1998 Nature 332:323-327; Jones, P. et al., 1986 Nature 321:522-525; Queen, C. et al., 1989 Proc. Natl. Acad., U.S.A. 86:10029-10033; U.S. Pat. No. 5,225,539 to Winter, and U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al.)
  • Accordingly, another embodiment of the invention pertains to an isolated monoclonal antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region comprising CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; CDR3 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237, respectively; and a light chain variable region having CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and CDR3 sequences consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies, yet may contain different framework sequences from these antibodies.
  • Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences. For example, germline DNA sequences for human heavy and light chain variable region genes can be found in the “VBase” human germline sequence database (available on the Internet at www.mrc-cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Tomlinson, I. M., et al., 1992 J. fol. Biol. 227:776-798; and Cox, J. P. L. et al., 1994 Eur. J Immunol. 24:827-836; the contents of each of which are expressly incorporated herein by reference.
  • An example of framework sequences for use in the antibodies of the invention are those that are structurally similar to the framework sequences used by selected antibodies of the invention, e.g., consensus sequences and/or framework sequences used by monoclonal antibodies of the invention. The VH CDR1, 2 and 3 sequences, and the VL CDR1, 2 and 3 sequences, can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences. For example, it has been found that in certain instances it is beneficial to mutate residues within the framework regions to maintain or enhance the antigen binding ability of the antibody (see e.g., U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al).
  • Another type of variable region modification is to mutate amino acid residues within the VH and/or VL CDR1, CDR2 and/or CDR3 regions to thereby improve one or more binding properties (e.g., affinity) of the antibody of interest, known as “affinity maturation.” Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples. Conservative modifications (as discussed above) can be introduced. The mutations may be amino acid substitutions, additions or deletions. Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered.
  • Accordingly, in another embodiment, the invention provides isolated C5-binding monoclonal antibodies, or an antigen binding fragment thereof, consisting of a heavy chain variable region having: a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235; a VH CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236; a VH CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237; a VL CDR1 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238; a VL CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239; and a VL CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240.
  • Grafting Antigen-Binding Domains into Alternative Frameworks or Scaffolds
  • A wide variety of antibody/immunoglobulin frameworks or scaffolds can be employed so long as the resulting polypeptide includes at least one binding region which specifically binds to C5. Such frameworks or scaffolds include the 5 main idiotypes of human immunoglobulins, or fragments thereof, and include immunoglobulins of other animal species, preferably having humanized aspects. Single heavy-chain antibodies such as those identified in camelids are of particular interest in this regard. Novel frameworks, scaffolds and fragments continue to be discovered and developed by those skilled in the art.
  • In one aspect, the invention pertains to generating non-immunoglobulin based antibodies using non-immunoglobulin scaffolds onto which CDRs of the invention can be grafted. Known or future non-immunoglobulin frameworks and scaffolds may be employed, as long as they comprise a binding region specific for the target C5 protein (e.g., human and/or cynomolgus C5). Known non-immunoglobulin frameworks or scaffolds include, but are not limited to, fibronectin (Compound Therapeutics, Inc., Waltham, Mass.), ankyrin (Molecular Partners AG, Zurich, Switzerland), domain antibodies (Domantis, Ltd., Cambridge, Mass., and Ablynx nv, Zwijnaarde, Belgium), lipocalin (Pieris Proteolab AG, Freising, Germany), small modular immuno-pharmaceuticals (Trubion Pharmaceuticals Inc., Seattle, Wash.), maxybodies (Avidia, Inc., Mountain View, Calif.), Protein A (Affibody AG, Sweden), and affilin (gamma-crystallin or ubiquitin) (Scil Proteins GmbH, Halle, Germany).
  • The fibronectin scaffolds are based on fibronectin type III domain (e.g., the tenth module of the fibronectin type III (10 Fn3 domain)). The fibronectin type III domain has 7 or 8 beta strands which are distributed between two beta sheets, which themselves pack against each other to form the core of the protein, and further containing loops (analogous to CDRs) which connect the beta strands to each other and are solvent exposed. There are at least three such loops at each edge of the beta sheet sandwich, where the edge is the boundary of the protein perpendicular to the direction of the beta strands (see U.S. Pat. No. 6,818,418). These fibronectin-based scaffolds are not an immunoglobulin, although the overall fold is closely related to that of the smallest functional antibody fragment, the variable region of the heavy chain, which comprises the entire antigen recognition unit in camel and llama IgG. Because of this structure, the non-immunoglobulin antibody mimics antigen binding properties that are similar in nature and affinity to those of antibodies. These scaffolds can be used in a loop randomization and shuffling strategy in vitro that is similar to the process of affinity maturation of antibodies in vivo. These fibronectin-based molecules can be used as scaffolds where the loop regions of the molecule can be replaced with CDRs of the invention using standard cloning techniques.
  • The ankyrin technology is based on using proteins with ankyrin derived repeat modules as scaffolds for bearing variable regions which can be used for binding to different targets. The ankyrin repeat module is a 33 amino acid polypeptide consisting of two anti-parallel α-helices and a β-turn. Binding of the variable regions is mostly optimized by using ribosome display.
  • Avimers are derived from natural A-domain containing protein such as LRP-1. These domains are used by nature for protein-protein interactions and in human over 250 proteins are structurally based on A-domains. Avimers consist of a number of different “A-domain” monomers (2-10) linked via amino acid linkers. Avimers can be created that can bind to the target antigen using the methodology described in, for example, U.S. Patent Application Publication Nos. 20040175756; 20050053973; 20050048512; and 20060008844.
  • Affibody affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold domain consists of 58 amino acids, 13 of which are randomized to generate affibody libraries with a large number of ligand variants (See e.g., U.S. Pat. No. 5,831,012). Affibody molecules mimic antibodies, they have a molecular weight of 6 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of affibody molecules is similar to that of an antibody.
  • Anticalins are products developed by the company Pieris ProteoLab AG. They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the physiological transport or storage of chemically sensitive or insoluble compounds. Several natural lipocalins occur in human tissues or body liquids. The protein architecture is reminiscent of immunoglobulins, with hypervariable loops on top of a rigid framework. However, in contrast with antibodies or their recombinant fragments, lipocalins are composed of a single polypeptide chain with 160 to 180 amino acid residues, being just marginally bigger than a single immunoglobulin domain. The set of four loops, which makes up the binding pocket, shows pronounced structural plasticity and tolerates a variety of side chains. The binding site can thus be reshaped in a proprietary process in order to recognize prescribed target molecules of different shape with high affinity and specificity. One protein of lipocalin family, the bilin-binding protein (BBP) of Pieris Brassicae has been used to develop anticalins by mutagenizing the set of four loops. One example of a patent application describing anticalins is in PCT Publication No. WO 199916873.
  • Affilin molecules are small non-immunoglobulin proteins which are designed for specific affinities towards proteins and small molecules. New affilin molecules can be very quickly selected from two libraries, each of which is based on a different human derived scaffold protein. Affilin molecules do not show any structural homology to immunoglobulin proteins. Currently, two affilin scaffolds are employed, one of which is gamma crystalline, a human structural eye lens protein and the other is “ubiquitin” superfamily proteins. Both human scaffolds are very small, show high temperature stability and are almost resistant to pH changes and denaturing agents. This high stability is mainly due to the expanded beta sheet structure of the proteins. Examples of gamma crystalline derived proteins are described in WO200104144 and examples of “ubiquitin-like” proteins are described in WO2004106368.
  • Protein epitope mimetics (PEM) are medium-sized, cyclic, peptide-like molecules (MW 1-2 kDa) mimicking beta-hairpin secondary structures of proteins, the major secondary structure involved in protein-protein interactions.
  • In some embodiments, the Fabs are converted to silent IgG1 format by changing the Fc region. For example, antibodies 6525-7910 in Table 1 can be converted to silent IgG1 formate by substituting the “X” in the amino acid sequences for the heavy chain with: CDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCWVDVSHEDPEVKFNWYVD GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKG QPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO: 293) and substituting the “X” in the amino acid sequence for the light chain with: CS if the light chain is lambda, or C if the light chain is kappa. As used herein, a “silent IgG1” is an IgG1 Fc sequence in which the amino acid sequence has been altered to reduce Fc-mediated effector functions (for example ADCC and/or CDC). Such an antibody will typically have reduced binding to Fc receptors and/or C1q.
  • In some other embodiments, the Fabs are converted to IgG2 format. For example, antibodies 6525-7910 in Table 1 can be converted to IgG2 format by substituting the constant sequence ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSX (SEQ ID NO: 294)
  • with the constant sequence for the heavy chain of IgG2: ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG LYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCCVECPPCPAPPVAGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTFRVV SVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTKGQPREPQVYTLPPSREEMTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLSPGK (SEQ ID NO: 295)
    and substituting the “X” in the amino acid sequence for the light chain with CS if the light chain is lambda, or C if the light chain is kappa.
    • Human or Humanized Antibodies
  • The present invention provides fully human antibodies that specifically bind to a C5 protein (e.g., human and/or cynomolgus C5). Compared to the chimeric or humanized antibodies, the human C5-binding antibodies of the invention have further reduced antigenicity when administered to human subjects.
  • The human C5-binding antibodies can be generated using methods that are known in the art. For example, the humaneering technology used to converting non-human antibodies into engineered human antibodies. U.S. Patent Publication No. 20050008625 describes an in vivo method for replacing a nonhuman antibody variable region with a human variable region in an antibody while maintaining the same or providing better binding characteristics relative to that of the nonhuman antibody. The method relies on epitope guided replacement of variable regions of a non-human reference antibody with a fully human antibody. The resulting human antibody is generally unrelated structurally to the reference nonhuman antibody, but binds to the same epitope on the same antigen as the reference antibody. Briefly, the serial epitope-guided complementarity replacement approach is enabled by setting up a competition in cells between a “competitor” and a library of diverse hybrids of the reference antibody (“test antibodies”) for binding to limiting amounts of antigen in the presence of a reporter system which responds to the binding of test antibody to antigen. The competitor can be the reference antibody or derivative thereof such as a single-chain Fv fragment. The competitor can also be a natural or artificial ligand of the antigen which binds to the same epitope as the reference antibody. The only requirements of the competitor are that it binds to the same epitope as the reference antibody, and that it competes with the reference antibody for antigen binding. The test antibodies have one antigen-binding V-region in common from the nonhuman reference antibody, and the other V-region selected at random from a diverse source such as a repertoire library of human antibodies. The common V-region from the reference antibody serves as a guide, positioning the test antibodies on the same epitope on the antigen, and in the same orientation, so that selection is biased toward the highest antigen-binding fidelity to the reference antibody.
  • Many types of reporter system can be used to detect desired interactions between test antibodies and antigen. For example, complementing reporter fragments may be linked to antigen and test antibody, respectively, so that reporter activation by fragment complementation only occurs when the test antibody binds to the antigen. When the test antibody- and antigen-reporter fragment fusions are co-expressed with a competitor, reporter activation becomes dependent on the ability of the test antibody to compete with the competitor, which is proportional to the affinity of the test antibody for the antigen. Other reporter systems that can be used include the reactivator of an auto-inhibited reporter reactivation system (RAIR) as disclosed in U.S. patent application Ser. No. 10/208,730 (Publication No. 20030198971), or competitive activation system disclosed in U.S. patent application Ser. No. 10/076,845 (Publication No. 20030157579).
  • With the serial epitope-guided complementarity replacement system, selection is made to identify cells expresses a single test antibody along with the competitor, antigen, and reporter components. In these cells, each test antibody competes one-on-one with the competitor for binding to a limiting amount of antigen. Activity of the reporter is proportional to the amount of antigen bound to the test antibody, which in turn is proportional to the affinity of the test antibody for the antigen and the stability of the test antibody. Test antibodies are initially selected on the basis of their activity relative to that of the reference antibody when expressed as the test antibody. The result of the first round of selection is a set of “hybrid” antibodies, each of which is comprised of the same non-human V-region from the reference antibody and a human V-region from the library, and each of which binds to the same epitope on the antigen as the reference antibody. One of more of the hybrid antibodies selected in the first round will have an affinity for the antigen comparable to or higher than that of the reference antibody.
  • In the second V-region replacement step, the human V-regions selected in the first step are used as guide for the selection of human replacements for the remaining non-human reference antibody V-region with a diverse library of cognate human V-regions. The hybrid antibodies selected in the first round may also be used as competitors for the second round of selection. The result of the second round of selection is a set of fully human antibodies which differ structurally from the reference antibody, but which compete with the reference antibody for binding to the same antigen. Some of the selected human antibodies bind to the same epitope on the same antigen as the reference antibody. Among these selected human antibodies, one or more binds to the same epitope with an affinity which is comparable to or higher than that of the reference antibody.
  • Using one of the mouse or chimeric C5-binding antibodies described above as the reference antibody, this method can be readily employed to generate human antibodies that bind to human C5 with the same binding specificity and the same or better binding affinity. In addition, such human C5-binding antibodies can also be commercially obtained from companies which customarily produce human antibodies, e.g., KaloBios, Inc. (Mountain View, Calif.).
    • Camelid Antibodies
  • Antibody proteins obtained from members of the camel and dromedary (Camelus bactrianus and Calelus dromaderius) family including new world members such as llama species (Lama paccos, Lama glama and Lama vicugna) have been characterized with respect to size, structural complexity and antigenicity for human subjects. Certain IgG antibodies from this family of mammals as found in nature lack light chains, and are thus structurally distinct from the typical four chain quaternary structure having two heavy and two light chains, for antibodies from other animals. See PCT/EP93/02214 (WO 94/04678 published 3 Mar. 1994).
  • A region of the camelid antibody which is the small single variable domain identified as VHH can be obtained by genetic engineering to yield a small protein having high affinity for a target, resulting in a low molecular weight antibody-derived protein known as a “camelid nanobody”. See U.S. Pat. No. 5,759,808 issued Jun. 2, 1998; see also Stijlemans, B. et al., 2004 J Biol Chem 279: 1256-1261; Dumoulin, M. et al., 2003 Nature 424: 783-788; Pleschberger, M. et al. 2003 Bioconjugate Chem 14: 440-448; Cortez-Retamozo, V. et al. 2002 Int J Cancer 89: 456-62; and Lauwereys, M. et al. 1998 EMBO J 17: 3512-3520. Engineered libraries of camelid antibodies and antibody fragments are commercially available, for example, from Ablynx, Ghent, Belgium. As with other antibodies of non-human origin, an amino acid sequence of a camelid antibody can be altered recombinantly to obtain a sequence that more closely resembles a human sequence, i.e., the nanobody can be “humanized”. Thus the natural low antigenicity of camelid antibodies to humans can be further reduced.
  • The camelid nanobody has a molecular weight approximately one-tenth that of a human IgG molecule, and the protein has a physical diameter of only a few nanometers. One consequence of the small size is the ability of camelid nanobodies to bind to antigenic sites that are functionally invisible to larger antibody proteins, i.e., camelid nanobodies are useful as reagents detect antigens that are otherwise cryptic using classical immunological techniques, and as possible therapeutic agents. Thus yet another consequence of small size is that a camelid nanobody can inhibit as a result of binding to a specific site in a groove or narrow cleft of a target protein, and hence can serve in a capacity that more closely resembles the function of a classical low molecular weight drug than that of a classical antibody.
  • The low molecular weight and compact size further result in camelid nanobodies being extremely thermostable, stable to extreme pH and to proteolytic digestion, and poorly antigenic. Another consequence is that camelid nanobodies readily move from the circulatory system into tissues, and even cross the blood-brain barrier and can treat disorders that affect nervous tissue. Nanobodies can further facilitated drug transport across the blood brain barrier. See U.S. patent application 20040161738 published Aug. 19, 2004. These features combined with the low antigenicity to humans indicate great therapeutic potential. Further, these molecules can be fully expressed in prokaryotic cells such as E. coli and are expressed as fusion proteins with bacteriophage and are functional.
  • Accordingly, a feature of the present invention is a camelid antibody or nanobody having high affinity for C5. In certain embodiments herein, the camelid antibody or nanobody is naturally produced in the camelid animal, i.e., is produced by the camelid following immunization with C5 or a peptide fragment thereof, using techniques described herein for other antibodies. Alternatively, the C5-binding camelid nanobody is engineered, i.e., produced by selection for example from a library of phage displaying appropriately mutagenized camelid nanobody proteins using panning procedures with C5 as a target as described in the examples herein. Engineered nanobodies can further be customized by genetic engineering to have a half life in a recipient subject of from 45 minutes to two weeks. In a specific embodiment, the camelid antibody or nanobody is obtained by grafting the CDRs sequences of the heavy or light chain of the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in PCT/EP93/02214.
    • Bispecific Molecules and Multivalent Antibodies
  • In another aspect, the present invention features bispecific or multispecific molecules comprising an C5-binding antibody, or a fragment thereof, of the invention. An antibody of the invention, or antigen-binding regions thereof, can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules. The antibody of the invention may in fact be derivatized or linked to more than one other functional molecule to generate multi-specific molecules that bind to more than two different binding sites and/or target molecules; such multi-specific molecules are also intended to be encompassed by the term “bispecific molecule” as used herein. To create a bispecific molecule of the invention, an antibody of the invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results.
  • Accordingly, the present invention includes bispecific molecules comprising at least one first binding specificity for C5 and a second binding specificity for a second target epitope. For example, the second target epitope is another epitope of C5 different from the first target epitope.
  • Additionally, for the invention in which the bispecific molecule is multi-specific, the molecule can further include a third binding specificity, in addition to the first and second target epitope.
  • In one embodiment, the bispecific molecules of the invention comprise as a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g., an Fab, Fab′, F(ab′)2, Fv, or a single chain Fv. The antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as described in Ladner et al. U.S. Pat. No. 4,946,778.
  • Diabodies are bivalent, bispecific molecules in which VH and VL domains are expressed on a single polypeptide chain, connected by a linker that is too short to allow for pairing between the two domains on the same chain. The VH and VL domains pair with complementary domains of another chain, thereby creating two antigen binding sites (see e.g., Holliger et al., 1993 Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak et al., 1994 Structure 2:1121-1123). Diabodies can be produced by expressing two polypeptide chains with either the structure VHA-VLB and VHB-VLA (VH-VL configuration), or VLA-VHB and VLB-VHA (VL-VH configuration) within the same cell. Most of them can be expressed in soluble form in bacteria. Single chain diabodies (scDb) are produced by connecting the two diabody-forming polypeptide chains with linker of approximately 15 amino acid residues (see Holliger and Winter, 1997 Cancer Immunol. Immunother., 45(3-4):128-30; Wu et al., 1996 Immunotechnology, 2(1):21-36). scDb can be expressed in bacteria in soluble, active monomeric form (see Holliger and Winter, 1997 Cancer Immunol. Immunother., 45(34): 128-30; Wu et al., 1996 Immunotechnology, 2(1):21-36; Pluckthun and Pack, 1997 Immunotechnology, 3(2): 83-105; Ridgway et al., 1996 Protein Eng., 9(7):617-21). A diabody can be fused to Fc to generate a “di-diabody” (see Lu et al., 2004 J. Biol. Chem., 279(4):2856-65).
  • Other antibodies which can be employed in the bispecific molecules of the invention are murine, chimeric and humanized monoclonal antibodies.
  • The bispecific molecules of the present invention can be prepared by conjugating the constituent binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross-linking agents can be used for covalent conjugation. Examples of cross-linking agents include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohaxane-I-carboxylate (sulfo-SMCC) (see e.g., Karpovsky et al., 1984 J. Exp. Med. 160:1686; Liu, M A et al., 1985 Proc. Natl. Acad. Sci. USA 82:8648). Other methods include those described in Paulus, 1985 Behring Ins. Mitt. No. 78, 118-132; Brennan et al., 1985 Science 229:81-83), and Glennie et al., 1987 J. Immunol. 139: 2367-2375). Conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, Ill.).
  • When the binding specificities are antibodies, they can be conjugated by sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains. In a particularly embodiment, the hinge region is modified to contain an odd number of sulfhydryl residues, for example one, prior to conjugation.
  • Alternatively, both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell. This method is particularly useful where the bispecific molecule is a mAb×mAb, mAb×Fab, Fab×F(ab′)2 or ligand×Fab fusion protein. A bispecific molecule of the invention can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants. Bispecific molecules may comprise at least two single chain molecules. Methods for preparing bispecific molecules are described for example in U.S. Pat. No. 5,260,203; U.S. Pat. No. 5,455,030; U.S. Pat. No. 4,881,175; U.S. Pat. No. 5,132,405; U.S. Pat. No. 5,091,513; U.S. Pat. No. 5,476,786; U.S. Pat. No. 5,013,653; U.S. Pat. No. 5,258,498; and U.S. Pat. No. 5,482,858.
  • Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (REA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay. Each of these assays generally detects the presence of protein-antibody complexes of particular interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest.
  • In another aspect, the present invention provides multivalent compounds comprising at least two identical or different antigen-binding portions of the antibodies of the invention binding to C5. The antigen-binding portions can be linked together via protein fusion or covalent or non covalent linkage. Alternatively, methods of linkage has been described for the bispecfic molecules. Tetravalent compounds can be obtained for example by cross-linking antibodies of the antibodies of the invention with an antibody that binds to the constant regions of the antibodies of the invention, for example the Fc or hinge region.
  • Trimerizing domain are described for example in Borean patent EP 1 012 280B1. Pentamerizing modules are described for example in PCT/EP97/05897.
  • Antibodies with Extended Half Life
  • The present invention provides for antibodies that specifically bind to C5 protein which have an extended half-life in vivo.
  • Many factors may affect a protein's half life in vivo. For examples, kidney filtration, metabolism in the liver, degradation by proteolytic enzymes (proteases), and immunogenic responses (e.g., protein neutralization by antibodies and uptake by macrophages and dentritic cells). A variety of strategies can be used to extend the half life of the antibodies of the present invention. For example, by chemical linkage to polyethyleneglycol (PEG), reCODE PEG, antibody scaffold, polysialic acid (PSA), hydroxyethyl starch (HES), albumin-binding ligands, and carbohydrate shields; by genetic fusion to proteins binding to serum proteins, such as albumin, IgG, FcRn, and transferring; by coupling (genetically or chemically) to other binding moieties that bind to serum proteins, such as nanobodies, Fabs, DARPins, avimers, affibodies, and anticalins; by genetic fusion to rPEG, albumin, domain of albumin, albumin-binding proteins, and Fc; or by incorporation into nancarriers, slow release formulations, or medical devices.
  • To prolong the serum circulation of antibodies in vivo, inert polymer molecules such as high molecular weight PEG can be attached to the antibodies or a fragment thereof with or without a multifunctional linker either through site-specific conjugation of the PEG to the N- or C-terminus of the antibodies or via epsilon-amino groups present on lysine residues. To pegylate an antibody, the antibody, or fragment thereof, typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment. The pegylation can be carried out by an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer). As used herein, the term “polyethylene glycol” is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (C1-C10) alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol-maleimide. In certain embodiments, the antibody to be pegylated is an aglycosylated antibody. Linear or branched polymer derivatization that results in minimal loss of biological activity will be used. The degree of conjugation can be closely monitored by SDS-PAGE and mass spectrometry to ensure proper conjugation of PEG molecules to the antibodies. Unreacted PEG can be separated from antibody-PEG conjugates by size-exclusion or by ion-exchange chromatography. PEG-derivatized antibodies can be tested for binding activity as well as for in vivo efficacy using methods well-known to those of skill in the art, for example, by immunoassays described herein. Methods for pegylating proteins are known in the art and can be applied to the antibodies of the invention. See for example, EP 0 154 316 by Nishimura et al. and EP 0 401 384 by Ishikawa et al.
  • Other modified pegylation technologies include reconstituting chemically orthogonal directed engineering technology (ReCODE PEG), which incorporates chemically specified side chains into biosynthetic proteins via a reconstituted system that includes tRNA synthetase and tRNA. This technology enables incorporation of more than 30 new amino acids into biosynthetic proteins in E. coli, yeast, and mammalian cells. The tRNA incorporates a nonnative amino acid any place an amber codon is positioned, converting the amber from a stop codon to one that signals incorporation of the chemically specified amino acid.
  • Recombinant pegylation technology (rPEG) can also be used for serum halflife extension. This technology involves genetically fusing a 300-600 amino acid unstructured protein tail to an existing pharmaceutical protein. Because the apparent molecular weight of such an unstructured protein chain is about 15-fold larger than its actual molecular weight, the serum halflife of the protein is greatly increased. In contrast to traditional PEGylation, which requires chemical conjugation and repurification, the manufacturing process is greatly simplified and the product is homogeneous.
  • Polysialytion is another technology, which uses the natural polymer polysialic acid (PSA) to prolong the active life and improve the stability of therapeutic peptides and proteins. PSA is a polymer of sialic acid (a sugar). When used for protein and therapeutic peptide drug delivery, polysialic acid provides a protective microenvironment on conjugation. This increases the active life of the therapeutic protein in the circulation and prevents it from being recognized by the immune system. The PSA polymer is naturally found in the human body. It was adopted by certain bacteria which evolved over millions of years to coat their walls with it. These naturally polysialylated bacteria were then able, by virtue of molecular mimicry, to foil the body's defense system. PSA, nature's ultimate stealth technology, can be easily produced from such bacteria in large quantities and with predetermined physical characteristics. Bacterial PSA is completely non-immunogenic, even when coupled to proteins, as it is chemically identical to PSA in the human body.
  • Another technology include the use of hydroxyethyl starch (“HES”) derivatives linked to antibodies. HES is a modified natural polymer derived from waxy maize starch and can be metabolized by the body's enzymes. HES solutions are usually administered to substitute deficient blood volume and to improve the rheological properties of the blood. Hesylation of an antibody enables the prolongation of the circulation half-life by increasing the stability of the molecule, as well as by reducing renal clearance, resulting in an increased biological activity. By varying different parameters, such as the molecular weight of HES, a wide range of HES antibody conjugates can be customized.
  • Antibodies having an increased half-life in vivo can also be generated introducing one or more amino acid modifications (i.e., substitutions, insertions or deletions) into an IgG constant domain, or FcRn binding fragment thereof (preferably a Fc or hinge Fc domain fragment). See, e.g., International Publication No. WO 98/23289; International Publication No. WO 97/34631; and U.S. Pat. No. 6,277,375.
  • Further, antibodies can be conjugated to albumin in order to make the antibody or antibody fragment more stable in vivo or have a longer half life in vivo. The techniques are well-known in the art, see, e.g., International Publication Nos. WO 93/15199, WO 93/15200, and WO 01/77137; and European Patent No. EP 413,622.
  • The strategies for increasing half life is especially useful in nanobodies, fibronectin-based binders, and other antibodies or proteins for which increased in vivo half life is desired.
    • Antibody Conjugates
  • The present invention provides antibodies or fragments thereof that specifically bind to a C5 protein recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to a heterologous protein or polypeptide (or fragment thereof, preferably to a polypeptide of at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90 or at least 100 amino acids) to generate fusion proteins. In particular, the invention provides fusion proteins comprising an antigen-binding fragment of an antibody described herein (e.g., a Fab fragment, Fd fragment, Fv fragment, F(ab)2 fragment, a VH domain, a VH CDR, a VL domain or a VL CDR) and a heterologous protein, polypeptide, or peptide. Methods for fusing or conjugating proteins, polypeptides, or peptides to an antibody or an antibody fragment are known in the art. See, e.g., U.S. Pat. Nos. 5,336,603, 5,622,929, 5,359,046, 5,349,053, 5,447,851, and 5,112,946; European Patent Nos. EP 307,434 and EP 367,166; International Publication Nos. WO 96/04388 and WO 91/06570; Ashkenazi et al., 1991, Proc. Natl. Acad. Sci. USA 88: 10535-10539; Zheng et al., 1995, J. Immunol. 154:5590-5600; and Vil et al., 1992, Proc. Natl. Acad. Sci. USA 89:11337-11341.
  • Additional fusion proteins may be generated through the techniques of gene-shuffling, motif-shuffling, exon-shuffling, and/or codon-shuffling (collectively referred to as “DNA shuffling”). DNA shuffling may be employed to alter the activities of antibodies of the invention or fragments thereof (e.g., antibodies or fragments thereof with higher affinities and lower dissociation rates). See, generally, U.S. Pat. Nos. 5,605,793, 5,811,238, 5,830,721, 5,834,252, and 5,837,458; Patten et al., 1997, Curr. Opinion Biotechnol. 8:724-33; Harayama, 1998, Trends Biotechnol. 16(2):76-82; Hansson, et al., 1999, J. Mol. Biol. 287:265-76; and Lorenzo and Blasco, 1998, Biotechniques 24(2):308-313 (each of these patents and publications are hereby incorporated by reference in its entirety). Antibodies or fragments thereof, or the encoded antibodies or fragments thereof, may be altered by being subjected to random mutagenesis by error-prone PCR, random nucleotide insertion or other methods prior to recombination. A polynucleotide encoding an antibody or fragment thereof that specifically binds to a C5 protein may be recombined with one or more components, motifs, sections, parts, domains, fragments, etc. of one or more heterologous molecules.
  • Moreover, the antibodies or fragments thereof can be fused to marker sequences, such as a peptide to facilitate purification. In preferred embodiments, the marker amino acid sequence is a hexa-histidine peptide, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, Calif., 91311), among others, many of which are commercially available. As described in Gentz et al., 1989, Proc. Natl. Acad. Sci. USA 86:821-824, for instance, hexa-histidine provides for convenient purification of the fusion protein. Other peptide tags useful for purification include, but are not limited to, the hemagglutinin (“HA”) tag, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., 1984, Cell 37:767), and the “flag” tag.
  • In other embodiments, antibodies of the present invention or fragments thereof conjugated to a diagnostic or detectable agent. Such antibodies can be useful for monitoring or prognosing the onset, development, progression and/or severity of a disease or disorder as part of a clinical testing procedure, such as determining the efficacy of a particular therapy. Such diagnosis and detection can accomplished by coupling the antibody to detectable substances including, but not limited to, various enzymes, such as, but not limited to, horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; prosthetic groups, such as, but not limited to, streptavidinlbiotin and avidin/biotin; fluorescent materials, such as, but not limited to, umbelliferone, fluorescein, fluorescein isothiocynate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; luminescent materials, such as, but not limited to, luminol; bioluminescent materials, such as but not limited to, luciferase, luciferin, and aequorin; radioactive materials, such as, but not limited to, iodine (131I, 125I, 123I, and 121I,), carbon (14C), sulfur (35S), tritium (3H), indium (115In, 113In, 112In, and 111In,), technetium (99Tc), thallium (201Ti), gallium (68Ga, 67Ga), palladium (103Pd), molybdenum (99Mo), xenon (133Xe), fluorine (18F), 153Sm, 177Lu, 159Gd, 149Pm, 140La, 175Yb, 166Ho, 90Y, 47Sc, 186Re, 188Re, 142Pr, 105Rh, 97Ru, 68Ge, 57Co, 65Zn, 85Sr, 32P, 153Gd, 169Yb, 51Cr, 54Mn, 75Se, 113Sn, and 117Tin; and positron emitting metals using various positron emission tomographies, and no radioactive paramagnetic metal ions.
  • The present invention further encompasses uses of antibodies or fragments thereof conjugated to a therapeutic moiety. An antibody or fragment thereof may be conjugated to a therapeutic moiety such as a cytotoxin, e.g., a cytostatic or cytocidal agent, a therapeutic agent or a radioactive metal ion, e.g., alpha-emitters. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells.
  • Further, an antibody or fragment thereof may be conjugated to a therapeutic moiety or drug moiety that modifies a given biological response. Therapeutic moieties or drug moieties are not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein, peptide, or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria toxin; a protein such as tumor necrosis factor, α-interferon, β-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator, an apoptotic agent, an anti-angiogenic agent; or, a biological response modifier such as, for example, a lymphokine.
  • Moreover, an antibody can be conjugated to therapeutic moieties such as a radioactive metal ion, such as alph-emiters such as 213Bi or macrocyclic chelators useful for conjugating radiometal ions, including but not limited to, 131In, 131LU, 131Y, 131Ho, 131Sm, to polypeptides. In certain embodiments, the macrocyclic chelator is 1,4,7,10-tetraazacyclododecane-N,N′,N″,N′″-tetraacetic acid (DOTA) which can be attached to the antibody via a linker molecule. Such linker molecules are commonly known in the art and described in Denardo et al., 1998, Clin Cancer Res. 4(10):2483-90; Peterson et al., 1999, Bioconjug. Chem. 10(4):553-7; and Zimmerman et al., 1999, Nucl. Med. Biol. 26(8):943-50, each incorporated by reference in their entireties.
  • Techniques for conjugating therapeutic moieties to antibodies are well known, see, e.g., Arnon et al., “Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy”, in Monoclonal Antibodies And Cancer Therapy, Reisfeld et al. (eds.), pp. 243-56 (Alan R. Liss, Inc. 1985); Hellstrom et al., “Antibodies For Drug Delivery”, in Controlled Drug Delivery (2nd Ed.), Robinson et al. (eds.), pp. 623-53 (Marcel Dekker, Inc. 1987); Thorpe, “Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review”, in Monoclonal Antibodies 84: Biological And Clinical Applications, Pinchera et al. (eds.), pp. 475-506 (1985); “Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy”, in Monoclonal Antibodies For Cancer Detection And Therapy, Baldwin et al. (eds.), pp. 303-16 (Academic Press 1985), and Thorpe et al., 1982, Immunol. Rev. 62:119-58.
  • Antibodies may also be attached to solid supports, which are particularly useful for immunoassays or purification of the target antigen. Such solid supports include, but are not limited to, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride or polypropylene.
    • 5.2. Methods of Producing Antibodies of the Invention 5.2.1. Nucleic Acids Encoding the Antibodies
  • The invention provides substantially purified nucleic acid molecules which encode polypeptides comprising segments or domains of the C5-binding antibody chains described above. Some of the nucleic acids of the invention comprise the nucleotide sequence encoding the heavy chain variable region shown in SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281, and/or the nucleotide sequence encoding the light chain variable region shown in SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289. In a specific embodiment, the nucleic acid molecules are those identified in Table 1. Some other nucleic acid molecules of the invention comprise nucleotide sequences that are substantially identical (e.g., at least 65, 80%, 95%, or 99%) to the nucleotide sequences of those identified in Table 1. When expressed from appropriate expression vectors, polypeptides encoded by these polynucleotides are capable of exhibiting C5 antigen binding capacity.
  • Also provided in the invention are polynucleotides which encode at least one CDR region and usually all three CDR regions from the heavy or light chain of the C5-binding antibody set forth above. Some other polynucleotides encode all or substantially all of the variable region sequence of the heavy chain and/or the light chain of the C5-binding antibody set forth above. Because of the degeneracy of the code, a variety of nucleic acid sequences will encode each of the immunoglobulin amino acid sequences.
  • The nucleic acid molecules of the invention can encode both a variable region and a constant region of the antibody. Some of nucleic acid sequences of the invention comprise nucleotides encoding a mature heavy chain variable region sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature heavy chain variable region sequence set forth in SEQ ID NO: 7, 23, 39, 51, 67, 79, 96, 108, 114, 121, 137, 151, 165, 179, 187, 201, 210, 218, 227, 241, 253, 257, 273, 277, or 281. Some other nucleic acid sequences comprising nucleotide encoding a mature light chain variable region sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature light chain variable region sequence set forth in SEQ ID NO: 8, 24, 40, 52, 68, 80, 90, 102, 122, 138, 152, 166, 180, 188, 202, 211, 219, 228, 242, 261, 265, 269, 285, or 289.
  • The polynucleotide sequences can be produced by de novo solid-phase DNA synthesis or by PCR mutagenesis of an existing sequence (e.g., sequences as described in the Examples below) encoding an C5-binding antibody or its binding fragment. Direct chemical synthesis of nucleic acids can be accomplished by methods known in the art, such as the phosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90; the phosphodiester method of Brown et al., Meth. Enzymol. 68:109, 1979; the diethylphosphoramidite method of Beaucage et al., Tetra. Lett., 22:1859, 1981; and the solid support method of U.S. Pat. No. 4,458,066. Introducing mutations to a polynucleotide sequence by PCR can be performed as described in, e.g., PCR Technology: Principles and Applications for DNA Amplification, H. A. Erlich (Ed.), Freeman Press, NY, NY, 1992; PCR Protocols: A Guide to Methods and Applications, Innis et al. (Ed.), Academic Press, San Diego, Calif., 1990; Mattila et al., Nucleic Acids Res. 19:967, 1991; and Eckert et al., PCR Methods and Applications 1:17, 1991.
  • Also provided in the invention are expression vectors and host cells for producing the C5-binding antibodies described above. Various expression vectors can be employed to express the polynucleotides encoding the C5-binding antibody chains or binding fragments. Both viral-based and nonviral expression vectors can be used to produce the antibodies in a mammalian host cell. Nonviral vectors and systems include plasmids, episomal vectors, typically with an expression cassette for expressing a protein or RNA, and human artificial chromosomes (see, e.g., Harrington et al., Nat Genet 15:345, 1997). For example, nonviral vectors useful for expression of the C5-binding polynucleotides and polypeptides in mammalian (e.g., human) cells include pThioHis A, B & C, pcDNA3.1/His, pEBVHis A, B & C, (Invitrogen, San Diego, Calif.), MPSV vectors, and numerous other vectors known in the art for expressing other proteins. Useful viral vectors include vectors based on retroviruses, adenoviruses, adenoassociated viruses, herpes viruses, vectors based on SV40, papilloma virus, HBP Epstein Barr virus, vaccinia virus vectors and Semliki Forest virus (SFV). See, Brent et al., supra; Smith, Annu. Rev. Microbiol. 49:807, 1995; and Rosenfeld et al., Cell 68:143, 1992.
  • The choice of expression vector depends on the intended host cells in which the vector is to be expressed. Typically, the expression vectors contain a promoter and other regulatory sequences (e.g., enhancers) that are operably linked to the polynucleotides encoding an C5-binding antibody chain or fragment. In some embodiments, an inducible promoter is employed to prevent expression of inserted sequences except under inducing conditions. Inducible promoters include, e.g., arabinose, lacZ, metallothionein promoter or a heat shock promoter. Cultures of transformed organisms can be expanded under noninducing conditions without biasing the population for coding sequences whose expression products are better tolerated by the host cells. In addition to promoters, other regulatory elements may also be required or desired for efficient expression of an C5-binding antibody chain or fragment. These elements typically include an ATG initiation codon and adjacent ribosome binding site or other sequences. In addition, the efficiency of expression may be enhanced by the inclusion of enhancers appropriate to the cell system in use (see, e.g., Scharf et al., Results Probl. Cell Differ. 20:125, 1994; and Bittner et al., Meth. Enzymol., 153:516, 1987). For example, the SV40 enhancer or CMV enhancer may be used to increase expression in mammalian host cells.
  • The expression vectors may also provide a secretion signal sequence position to form a fusion protein with polypeptides encoded by inserted C5-binding antibody sequences. More often, the inserted C5-binding antibody sequences are linked to a signal sequences before inclusion in the vector. Vectors to be used to receive sequences encoding C5-binding antibody light and heavy chain variable domains sometimes also encode constant regions or parts thereof. Such vectors allow expression of the variable regions as fusion proteins with the constant regions thereby leading to production of intact antibodies or fragments thereof. Typically, such constant regions are human.
  • The host cells for harboring and expressing the C5-binding antibody chains can be either prokaryotic or eukaryotic. E. coli is one prokaryotic host useful for cloning and expressing the polynucleotides of the present invention. Other microbial hosts suitable for use include bacilli, such as Bacillus subtilis, and other enterobacteriaceae, such as Salmonella, Serratia, and various Pseudomonas species. In these prokaryotic hosts, one can also make expression vectors, which typically contain expression control sequences compatible with the host cell (e.g., an origin of replication). In addition, any number of a variety of well-known promoters will be present, such as the lactose promoter system, a tryptophan (trp) promoter system, a beta-lactamase promoter system, or a promoter system from phage lambda. The promoters typically control expression, optionally with an operator sequence, and have ribosome binding site sequences and the like, for initiating and completing transcription and translation. Other microbes, such as yeast, can also be employed to express C5-binding polypeptides of the invention. Insect cells in combination with baculovirus vectors can also be used.
  • In some preferred embodiments, mammalian host cells are used to express and produce the C5-binding polypeptides of the present invention. For example, they can be either a hybridoma cell line expressing endogenous immunoglobulin genes (e.g., the 1D6.C9 myeloma hybridoma clone as described in the Examples) or a mammalian cell line harboring an exogenous expression vector (e.g., the SP2/0 myeloma cells exemplified below). These include any normal mortal or normal or abnormal immortal animal or human cell. For example, a number of suitable host cell lines capable of secreting intact immunoglobulins have been developed including the CHO cell lines, various Cos cell lines, HeLa cells, myeloma cell lines, transformed B-cells and hybridomas. The use of mammalian tissue cell culture to express polypeptides is discussed generally in, e.g., Winnacker, FROM GENES TO CLONES, VCH Publishers, N.Y., N.Y., 1987. Expression vectors for mammalian host cells can include expression control sequences, such as an origin of replication, a promoter, and an enhancer (see, e.g., Queen, et al., Immunol. Rev. 89:49-68, 1986), and necessary processing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcriptional terminator sequences. These expression vectors usually contain promoters derived from mammalian genes or from mammalian viruses. Suitable promoters may be constitutive, cell type-specific, stage-specific, and/or modulatable or regulatable. Useful promoters include, but are not limited to, the metallothionein promoter, the constitutive adenovirus major late promoter, the dexamethasone-inducible MMTV promoter, the SV40 promoter, the MRP poIIII promoter, the constitutive MPSV promoter, the tetracycline-inducible CMV promoter (such as the human immediate-early CMV promoter), the constitutive CMV promoter, and promoter-enhancer combinations known in the art.
  • Methods for introducing expression vectors containing the polynucleotide sequences of interest vary depending on the type of cellular host. For example, calcium chloride transfection is commonly utilized for prokaryotic cells, whereas calcium phosphate treatment or electroporation may be used for other cellular hosts. (See generally Sambrook, et al., supra). Other methods include, e.g., electroporation, calcium phosphate treatment, liposome-mediated transformation, injection and microinjection, ballistic methods, virosomes, immunoliposomes, polycation:nucleic acid conjugates, naked DNA, artificial virions, fusion to the herpes virus structural protein VP22 (Elliot and O'Hare, Cell 88:223, 1997), agent-enhanced uptake of DNA, and ex vivo transduction. For long-term, high-yield production of recombinant proteins, stable expression will often be desired. For example, cell lines which stably express C5-binding antibody chains or binding fragments can be prepared using expression vectors of the invention which contain viral origins of replication or endogenous expression elements and a selectable marker gene. Following the introduction of the vector, cells may be allowed to grow for 1-2 days in an enriched media before they are switched to selective media. The purpose of the selectable marker is to confer resistance to selection, and its presence allows growth of cells which successfully express the introduced sequences in selective media. Resistant, stably transfected cells can be proliferated using tissue culture techniques appropriate to the cell type.
    • 5.2.2. Generation of Monoclonal Antibodies of the Invention
  • Monoclonal antibodies (mAbs) can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein, 1975 Nature 256: 495. Many techniques for producing monoclonal antibody can be employed e.g., viral or oncogenic transformation of B lymphocytes.
  • An animal system for preparing hybridomas is the murine system. Hybridoma production in the mouse is a well established procedure. Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.
  • Chimeric or humanized antibodies of the present invention can be prepared based on the sequence of a murine monoclonal antibody prepared as described above. DNA encoding the heavy and light chain immunoglobulins can be obtained from the murine hybridoma of interest and engineered to contain non-murine (e.g., human) immunoglobulin sequences using standard molecular biology techniques. For example, to create a chimeric antibody, the murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Pat. No. 4,816,567 to Cabilly et al.). To create a humanized antibody, the murine CDR regions can be inserted into a human framework using methods known in the art. See e.g., U.S. Pat. No. 5,225,539 to Winter, and U.S. Pat. Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al.
  • In a certain embodiment, the antibodies of the invention are human monoclonal antibodies. Such human monoclonal antibodies directed against C5 can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system. These transgenic and transchromosomic mice include mice referred to herein as HuMAb mice and KM mice, respectively, and are collectively referred to herein as “human Ig mice.”
  • The HuMAb Mouse® (Medarex, Inc.) contains human immunoglobulin gene miniloci that encode un-rearranged human heavy (μ and γ) and κ light chain immunoglobulin sequences, together with targeted mutations that inactivate the endogenous μ and κ chain loci (see e.g., Lonberg, et al., 1994 Nature 368(6474): 856-859). Accordingly, the mice exhibit reduced expression of mouse IgM or κ, and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human IgGκ monoclonal (Lonberg, N. et al., 1994 supra; reviewed in Lonberg, N., 1994 Handbook of Experimental Pharmacology 113:49-101; Lonberg, N. and Huszar, D., 1995 Intern. Rev. Immunol. 13: 65-93, and Harding, F. and Lonberg, N., 1995 Ann. N. Y. Acad. Sci. 764:536-546). The preparation and use of HuMAb mice, and the genomic modifications carried by such mice, is further described in Taylor, L. et al., 1992 Nucleic Acids Research 20:6287-6295; Chen, J. et at., 1993 International Immunology 5: 647-656; Tuaillon et al., 1993 Proc. Natl. Acad. Sci. USA 94:3720-3724; Choi et al., 1993 Nature Genetics 4:117-123; Chen, J. et al., 1993 EMBO J. 12: 821-830; Tuaillon et al., 1994 J. Immunol. 152:2912-2920; Taylor, L. et al., 1994 International Immunology 579-591; and Fishwild, D. et al., 1996 Nature Biotechnology 14: 845-851, the contents of all of which are hereby specifically incorporated by reference in their entirety. See further, U.S. Pat. Nos. 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,789,650; 5,877,397; 5,661,016; 5,814,318; 5,874,299; and 5,770,429; all to Lonberg and Kay; U.S. Pat. No. 5,545,807 to Surani et al.; PCT Publication Nos. WO 92103918, WO 93/12227, WO 94/25585, WO 97113852, WO 98/24884 and WO 99/45962, all to Lonberg and Kay; and PCT Publication No. WO 01/14424 to Korman et al.
  • In another embodiment, human antibodies of the invention can be raised using a mouse that carries human immunoglobulin sequences on transgenes and transchomosomes such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome. Such mice, referred to herein as “KM mice”, are described in detail in PCT Publication WO 02/43478 to Ishida et al.
  • Still further, alternative transgenic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise C5-binding antibodies of the invention. For example, an alternative transgenic system referred to as the Xenomouse (Abgenix, Inc.) can be used. Such mice are described in, e.g., U.S. Pat. Nos. 5,939,598; 6,075,181; 6,114,598; 6, 150,584 and 6,162,963 to Kucherlapati et al.
  • Moreover, alternative transchromosomic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise C5-binding antibodies of the invention. For example, mice carrying both a human heavy chain transchromosome and a human light chain tranchromosome, referred to as “TC mice” can be used; such mice are described in Tomizuka et al., 2000 Proc. Natl. Acad. Sci. USA 97:722-727. Furthermore, cows carrying human heavy and light chain transchromosomes have been described in the art (Kuroiwa et al., 2002 Nature Biotechnology 20:889-894) and can be used to raise C5-binding antibodies of the invention.
  • Human monoclonal antibodies of the invention can also be prepared using phage display methods for screening libraries of human immunoglobulin genes. Such phage display methods for isolating human antibodies are established in the art or described in the examples below. See for example: U.S. Pat. Nos. 5,223,409; 5,403,484; and 5,571,698 to Ladner et al.; U.S. Pat. Nos. 5,427,908 and 5,580,717 to Dower et al.; U.S. Pat. Nos. 5,969,108 and 6,172,197 to McCafferty et al.; and U.S. Pat. Nos. 5,885,793; 6,521,404; 6,544,731; 6,555,313; 6,582,915 and 6,593,081 to Griffiths et al.
  • Human monoclonal antibodies of the invention can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization. Such mice are described in, for example, U.S. Pat. Nos. 5,476,996 and 5,698,767 to Wilson et al.
    • 5.2.3. Framework or Fc Engineering
  • Engineered antibodies of the invention include those in which modifications have been made to framework residues within VH and/or VL, e.g. to improve the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody. For example, one approach is to “backmutate” one or more framework residues to the corresponding germline sequence. More specifically, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germline sequences from which the antibody is derived. To return the framework region sequences to their germline configuration, the somatic mutations can be “backmutated” to the germline sequence by, for example, site-directed mutagenesis. Such “backmutated” antibodies are also intended to be encompassed by the invention.
  • Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell-epitopes to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as “deimmunization” and is described in further detail in U.S. Patent Publication No. 20030153043 by Carr et al.
  • In addition or alternative to modifications made within the framework or CDR regions, antibodies of the invention may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity. Furthermore, an antibody of the invention may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody. Each of these embodiments is described in further detail below. The numbering of residues in the Fc region is that of the EU index of Kabat.
  • In one embodiment, the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased. This approach is described further in U.S. Pat. No. 5,677,425 by Bodmer et al. The number of cysteine residues in the hinge region of CH1 is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
  • In another embodiment, the Fc hinge region of an antibody is mutated to decrease the biological half-life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc-hinge fragment such that the antibody has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding. This approach is described in further detail in U.S. Pat. No. 6,165,745 by Ward et al.
  • In another embodiment, the antibody is modified to increase its biological half-life. Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Pat. No. 6,277,375 to Ward. Alternatively, to increase the biological half life, the antibody can be altered within the CH1 or CL region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Pat. Nos. 5,869,046 and 6,121,022 by Presta et al.
  • In yet other embodiments, the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector functions of the antibody. For example, one or more amino acids can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen-binding ability of the parent antibody. The effector ligand to which affinity is altered can be, for example, an Fc receptor or the C1 component of complement. This approach is described in further detail in U.S. Pat. Nos. 5,624,821 and 5,648,260, both by Winter et al.
  • In another embodiment, one or more amino acids selected from amino acid residues can be replaced with a different amino acid residue such that the antibody has altered C1q binding and/or reduced or abolished complement dependent cytotoxicity (CDC). This approach is described in further detail in U.S. Pat. No. 6,194,551 by Idusogie et al.
  • In another embodiment, one or more amino acid residues are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
  • In yet another embodiment, the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fcγ receptor by modifying one or more amino acids. This approach is described further in PCT Publication WO 00/42072 by Presta. Moreover, the binding sites on human IgG1 for FcγRI, FcγRII, FcγRIII and FcRn have been mapped and variants with improved binding have been described (see Shields, R. L. et al., 2001 J. Biol. Chen. 276:6591-6604).
  • In still another embodiment, the glycosylation of an antibody is modified. For example, an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation). Glycosylation can be altered to, for example, increase the affinity of the antibody for “antigen’. Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence. For example, one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site. Such aglycosylation may increase the affinity of the antibody for antigen. Such an approach is described in further detail in U.S. Pat. Nos. 5,714,350 and 6,350,861 by Co et al.
  • Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies. Such carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation. For example, EP 1,176,195 by Hang et al. describes a cell line with a functionally disrupted FUT8 gene, which encodes a fucosyl transferase, such that antibodies expressed in such a cell line exhibit hypofucosylation. PCT Publication WO 03/035835 by Presta describes a variant CHO cell line, Lecl3 cells, with reduced ability to attach fucose to Asn(297)-linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, R. L. et al., 2002 J. Biol. Chem. 277:26733-26740). PCT Publication WO 99/54342 by Umana et al. describes cell lines engineered to express glycoprotein-modifying glycosyl transferases (e.g., beta(1,4)-N acetylglucosaminyltransferase III (GnTIII)) such that antibodies expressed in the engineered cell lines exhibit increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana et al., 1999 Nat. Biotech. 17:176-180).
    • 5.2.4. Methods of Engineering Altered Antibodies
  • As discussed above, the C5-binding antibodies having VH and VL sequences or full length heavy and light chain sequences shown herein can be used to create new C5-binding antibodies by modifying full length heavy chain and/or light chain sequences, VH and/or VL sequences, or the constant region(s) attached thereto. Thus, in another aspect of the invention, the structural features of a C5-binding antibody of the invention are used to create structurally related C5-binding antibodies that retain at least one functional property of the antibodies of the invention, such as binding to human C5 and also inhibiting one or more functional properties of C5 (e.g., inhibit red blood cell lysis in a hemolytic assay).
  • For example, one or more CDR regions of the antibodies of the present invention, or mutations thereof, can be combined recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, C5-binding antibodies of the invention, as discussed above. Other types of modifications include those described in the previous section. The starting material for the engineering method is one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. To create the engineered antibody, it is not necessary to actually prepare (i.e., express as a protein) an antibody having one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. Rather, the information contained in the sequence(s) is used as the starting material to create a “second generation” sequence(s) derived from the original sequence(s) and then the “second generation” sequence(s) is prepared and expressed as a protein.
  • Accordingly, in another embodiment, the invention provides a method for preparing an C5-binding antibody consisting of: a heavy chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 1, 17, 33, 61, 131, 145, 159, 173, 195, and 235, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 2, 18, 34, 49, 62, 77, 95, 107, 113, 119, 132, 146, 160, 174, 196, 226, and 236, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 3, 19, 35, 63, 133, 147, 161, 175, 197, and 237; and a light chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 4, 20, 36, 64, 134, 148, 162, 176, 198, and 238, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 5, 21, 37, 65, 135, 149, 163, 177, 199, and 239, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 6, 22, 38, 50, 66, 78, 89, 101, 120, 136, 150, 164, 178, 200, 209, and 240; altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.
  • Accordingly, in another embodiment, the invention provides a method for preparing an C5-binding antibody optimized for expression in a mammalian cell consisting of: a full length heavy chain antibody sequence having a sequence selected from the group of SEQ ID NOs: 9, 25, 41, 53, 69, 81, 97, 109, 115, 123, 139, 153, 167, 181, 189, 203, 212, 220, 229, 243, 249, 254, 258, 274, 278, and 282; and a full length light chain antibody sequence having a sequence selected from the group of 10, 26, 42, 54, 70, 82, 91, 103, 124, 140, 154, 168, 182, 190, 204, 213, 221, 230, 244, 251, 262, 266, 270, 286, and 290; altering at least one amino acid residue within the full length heavy chain antibody sequence and/or the full length light chain antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.
  • The altered antibody sequence can also be prepared by screening antibody libraries having fixed CDR3 sequences or minimal essential binding determinants as described in US20050255552 and diversity on CDR1 and CDR2 sequences. The screening can be performed according to any screening technology appropriate for screening antibodies from antibody libraries, such as phage display technology.
  • Standard molecular biology techniques can be used to prepare and express the altered antibody sequence. The antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the functional properties of the C5-binding antibodies described herein, which functional properties include, but are not limited to, specifically binding to human and/or cynomolgus C5; and the antibody inhibit red blood cell lysis in a hemolytic assay.
  • The functional properties of the altered antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g., ELISAs).
  • In certain embodiments of the methods of engineering antibodies of the invention, mutations can be introduced randomly or selectively along all or part of an C5-binding antibody coding sequence and the resulting modified C5-binding antibodies can be screened for binding activity and/or other functional properties as described herein. Mutational methods have been described in the art. For example, PCT Publication WO 02/092780 by Short describes methods for creating and screening antibody mutations using saturation mutagenesis, synthetic ligation assembly, or a combination thereof. Alternatively, PCT Publication WO 03/074679 by Lazar et al. describes methods of using computational screening methods to optimize physiochemical properties of antibodies.
    • 5.3. Characterization of the Antibodies of the Invention
  • The antibodies of the invention can be characterized by various functional assays. For example, they can be characterized by their ability to inhibit red blood cell lysis in hemolytic assays, their affinity to a C5 protein (e.g., human and/or cynomolgus C5), the epitope binning, their resistance to proteolysis, and their ability to block the complement cascade, for example, their ability to inhibit MAC formation.
  • Various methods can be used to measure presence of complement pathway molecules and activation of the complement system (see, e.g., U.S. Pat. No. 6,087,120; and Newell et al., J Lab Clin Med, 100:437-44, 1982). For example, the complement activity can be monitored by (i) measurement of inhibition of complement-mediated lysis of red blood cells (hemolysis); (ii) measurement of ability to inhibit cleavage of C3 or C5; and (iii) inhibition of alternative pathway mediated hemolysis.
  • The two most commonly used techniques are hemolytic assays (see, e.g., Baatrup et al., Ann Rheum Dis, 51:892-7, 1992) and immunological assays (see, e.g., Auda et al., Rheumatol Int, 10:185-9, 1990). The hemolytic techniques measure the functional capacity of the entire sequence-either the classical or alternative pathway. Immunological techniques measure the protein concentration of a specific complement component or split product. Other assays that can be employed to detect complement activation or measure activities of complement components in the methods of the present invention include, e.g., T cell proliferation assay (Chain et al., J Immunol Methods, 99:221-8, 1987), and delayed type hypersensitivity (DTH) assay (Forstrom et al., 1983, Nature 303:627-629; Halliday et al., 1982, in Assessment of Immune Status by the Leukocyte Adherence Inhibition Test, Academic, New York pp. 1-26; Koppi et al., 1982, Cell. Immunol. 66:394-406; and U.S. Pat. No. 5,843,449).
  • In hemolytic techniques, all of the complement components must be present and functional. Therefore hemolytic techniques can screen both functional integrity and deficiencies of the complement system (see, e.g., Dijk et al., J Immunol Methods 36: 29-39, 1980; Minh et al., Clin Lab Haematol. 5:23-34 1983; and Tanaka et al., J Immunol 86: 161-170, 1986). To measure the functional capacity of the classical pathway, sheep red blood cells coated with hemolysin (rabbit IgG to sheep red blood cells) or chicken red blood cells that are sensitized with rabbit anti-chicken antibodies are used as target cells (sensitized cells). These Ag-Ab complexes activate the classical pathway and result in lysis of the target cells when the components are functional and present in adequate concentration. To determine the functional capacity of the alternative pathway, rabbit red blood cells are used as the target cell (see, e.g., U.S. Pat. No. 6,087,120).
  • To test the ability of an antibody to inhibit MAC (membrance attack complex) formation, a MAC deposition assay can be performed. Briefly, zymosan can be used to activate the alternative pathway and IgM can be used to active the classic pathway. Fabs are pre-inclubated with human serum and added to plates coated with zymosan or IgM. Percentage inhibition of MAC deposition can be calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum).
  • The ability of an antibody to bind to C5 can be detected by labelling the antibody of interest directly, or the antibody may be unlabelled and binding detected indirectly using various sandwich assay formats known in the art.
  • In some embodiments, the C5-binding antibodies of the invention block or compete with binding of a reference C5-binding antibody to a C5 polypeptide. These can be fully human C5-binding antibodies described above. They can also be other mouse, chimeric or humanized C5-binding antibodies which bind to the same epitope as the reference antibody. The capacity to block or compete with the reference antibody binding indicates that a C5-binding antibody under test binds to the same or similar epitope as that defined by the reference antibody, or to an epitope which is sufficiently proximal to the epitope bound by the reference C5-binding antibody. Such antibodies are especially likely to share the advantageous properties identified for the reference antibody. The capacity to block or compete with the reference antibody may be determined by, e.g., a competition binding assay. With a competition binding assay, the antibody under test is examined for ability to inhibit specific binding of the reference antibody to a common antigen, such as a C5 polypeptide. A test antibody competes with the reference antibody for specific binding to the antigen if an excess of the test antibody substantially inhibits binding of the reference antibody. Substantial inhibition means that the test antibody reduces specific binding of the reference antibody usually by at least 10%, 25%, 50%, 75%, or 90%.
  • There are a number of known competition binding assays that can be used to assess competition of a C5-binding antibody with the reference C5-binding antibody for binding to a C5 protein. These include, e.g., solid phase direct or indirect radioimmunoassay (RIA), solid phase direct or indirect enzyme immunoassay (EIA), sandwich competition assay (see Stahli et al., Methods in Enzymology 9:242-253, 1983); solid phase direct biotin-avidin EIA (see Kirkland et al., J. Immunol. 137:3614-3619, 1986); solid phase direct labeled assay, solid phase direct labeled sandwich assay (see Harlow & Lane, supra); solid phase direct label RIA using 1-125 label (see Morel et al., Molec. Immunol. 25:7-15, 1988); solid phase direct biotin-avidin EIA (Cheung et al., Virology 176:546-552, 1990); and direct labeled RIA (Moldenhauer et al., Scand. J. Immunol. 32:77-82, 1990). Typically, such an assay involves the use of purified antigen bound to a solid surface or cells bearing either of these, an unlabelled test C5-binding antibody and a labelled reference antibody. Competitive inhibition is measured by determining the amount of label bound to the solid surface or cells in the presence of the test antibody. Usually the test antibody is present in excess. Antibodies identified by competition assay (competing antibodies) include antibodies binding to the same epitope as the reference antibody and antibodies binding to an adjacent epitope sufficiently proximal to the epitope bound by the reference antibody for steric hindrance to occur.
  • To determine if the selected C5-binding monoclonal antibodies bind to unique epitopes, each antibody can be biotinylated using commercially available reagents (e.g., reagents from Pierce, Rockford, Ill.). Competition studies using unlabeled monoclonal antibodies and biotinylated monoclonal antibodies can be performed using a C5 polypeptide coated-ELISA plates. Biotinylated MAb binding can be detected with a strep-avidin-alkaline phosphatase probe. To determine the isotype of a purified C5-binding antibody, isotype ELISAs can be performed. For example, wells of microtiter plates can be coated with 1 μg/ml of anti-human IgG overnight at 4° C. After blocking with 1% BSA, the plates are reacted with 1 μg/ml or less of the monoclonal C5-binding antibody or purified isotype controls, at ambient temperature for one to two hours. The wells can then be reacted with either human IgGI or human IgM-specific alkaline phosphatase-conjugated probes. Plates are then developed and analyzed so that the isotype of the purified antibody can be determined.
  • To demonstrate binding of monoclonal C5-binding antibodies to live cells expressing a C5 polypeptide, flow cytometry can be used. Briefly, cell lines expressing C5 (grown under standard growth conditions) can be mixed with various concentrations of a C5-binding antibody in PBS containing 0.1% BSA and 10% fetal calf serum, and incubated at 37° C. for 1 hour. After washing, the cells are reacted with Fluorescein-labeled anti-human IgG antibody under the same conditions as the primary antibody staining. The samples can be analyzed by FACScan instrument using light and side scatter properties to gate on single cells. An alternative assay using fluorescence microscopy may be used (in addition to or instead of) the flow cytometry assay. Cells can be stained exactly as described above and examined by fluorescence microscopy. This method allows visualization of individual cells, but may have diminished sensitivity depending on the density of the antigen.
  • C5-binding antibodies of the invention can be further tested for reactivity with a C5 polypeptide or antigenic fragment by Western blotting. Briefly, purified C5 polypeptides or fusion proteins, or cell extracts from cells expressing C5 can be prepared and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. After electrophoresis, the separated antigens are transferred to nitrocellulose membranes, blocked with 10% fetal calf serum, and probed with the monoclonal antibodies to be tested. Human IgG binding can be detected using anti-human IgG alkaline phosphatase and developed with BCIP/NBT substrate tablets (Sigma Chem. Co., St. Louis, Mo.).
  • Examples of functional assays are also described in the Example section below.
    • 5.4. Prophylactic and Therapeutic Uses
  • The present invention provides methods of treating a disease or disorder associated with increased complement activity by administering to a subject in need thereof an effective amount of the antibodies of the invention. In a specific embodiment, the present invention provides a method of treating age-related macular degeneration (AMD) by administering to a subject in need thereof an effective amount of the antibodies of the invention.
  • The antibodies of the invention can be used, inter alia, to prevent progression of dry AMD to wet AMD, to slow and/or prevent progression of geographic atrophy, and to improve vision lost due to dry AMD progression. It can also be used in combination with anti-VEGF therapies for the treatment of wet AMD patients.
  • In some embodiments, the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention. Examples of known complement related diseases or disorders include: neurological disorders, multiple sclerosis, stroke, Guillain Barre Syndrome, traumatic brain injury, Parkinson's disease, disorders of inappropriate or undesirable complement activation, hemodialysis complications, hyperacute allograft rejection, xenograft rejection, interleukin-2 induced toxicity during IL-2 therapy, inflammatory disorders, inflammation of autoimmune diseases, Crohn's disease, adult respiratory distress syndrome, thermal injury including burns or frostbite, post-ischemic reperfusion conditions, myocardial infarction, balloon angioplasty, post-pump syndrome in cardiopulmonary bypass or renal bypass, hemodialysis, renal ischemia, mesenteric artery reperfusion after acrotic reconstruction, infectious disease or sepsis, immune complex disorders and autoimmune diseases, rheumatoid arthritis, systemic lupus erythematosus (SLE), SLE nephritis, proliferative nephritis, hemolytic anemia, and myasthenia gravis. In addition, other known complement related disease are lung disease and disorders such as dyspnea, hemoptysis, ARDS, asthma, chronic obstructive pulmonary disease (COPD), emphysema, pulmonary embolisms and infarcts, pneumonia, fibrogenic dust diseases, inert dusts and minerals (e.g., silicon, coal dust, beryllium, and asbestos), pulmonary fibrosis, organic dust diseases, chemical injury (due to irritant gasses and chemicals, e.g., chlorine, phosgene, sulfur dioxide, hydrogen sulfide, nitrogen dioxide, ammonia, and hydrochloric acid), smoke injury, thermal injury (e.g., burn, freeze), asthma, allergy, bronchoconstriction, hypersensitivity pneumonitis, parasitic diseases, Goodpasture's Syndrome, pulmonary vasculitis, and immune complex-associated inflammation.
  • In a specific embodiment, the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention, wherein said disease or disorder is asthma, arthritis (e.g., rheumatoid arthritis), autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer-Simons Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, diseases of the central nervous system such as Alzheimer's disease and other neurodegenerative conditions, aHUS, glomerulonephritis, bullous pemphigoid or MPGN II.
  • In a specific embodiment, the present invention provides methods of treating glomerulonephritis by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. Symptoms of glomerulonephritis include, but not limited to, proteinuria; reduced glomerular filtration rate (GFR); serum electrolyte changes including azotemia (uremia, excessive blood urea nitrogen—BUN) and salt retention, leading to water retention resulting in hypertension and edema; hematuria and abnormal urinary sediments including red cell casts; hypoalbuminemia; hyperlipidemia; and lipiduria. In a specific embodiment, the present invention provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention.
  • In a specific embodiment, the present invention provides methods of reducing the dysfunction of the immune and hemostatic systems associated with extracorporeal circulation by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. The antibodies of the present invention can be used in any procedure which involves circulating the patient's blood from a blood vessel of the patient, through a conduit, and back to a blood vessel of the patient, the conduit having a luminal surface comprising a material capable of causing at least one of complement activation, platelet activation, leukocyte activation, or platelet-leukocyte adhesion. Such procedures include, but are not limited to, all forms of ECC, as well as procedures involving the introduction of an artificial or foreign organ, tissue, or vessel into the blood circuit of a patient.
  • Subjects to be treated with therapeutic agents of the present invention can also be administered other therapeutic agents with know methods of treating conditions associated with macular degeneration, such as antibiotic treatments as described in U.S. Pat. No. 6,218,368. In other treatments, immunosuppressive agents such as cyclosporine, are agents capable of suppressing immune responses. These agents include cytotoxic drugs, corticosteriods, nonsteroidal anti-inflammatory drugs (NSAIDs), specific T-lymphocyte immunosuppressants, and antibodies or fragments thereof (see Physicians' Desk Reference, 53rd edition, Medical Economics Company Inc., Montvale, N.J. (1999). Immunosuppressive treatment is typically continued at intervals for a period of a week, a month, three months, six months or a year. In some patients, treatment is administered for up to the rest of a patient's life.
  • When the therapeutic agents of the present invention are administered together with another agent, the two can be administered sequentially in either order or simultaneously. In some aspects, an antibody of the present invention is administered to a subject who is also receiving therapy with a second agent (e.g., verteporfin). In other aspects, the binding molecule is administered in conjunction with surgical treatments.
  • Suitable agents for combination treatment with C5-binding antibodies include agents known in the art that are able to modulate the activities of complement components (see, e.g., U.S. Pat. No. 5,808,109). Other agents have been reported to diminish complement-mediated activity. Such agents include: amino acids (Takada, Y. et al. Immunology 1978, 34, 509); phosphonate esters (Becker, L. Biochem. Biophy. Acta 1967, 147, 289); polyanionic substances (Conrow, R. B. et al. J. Med. Chem. 1980, 23, 242); sulfonyl fluorides (Hansch, C.; Yoshimoto, M. J. Med. Chem. 1974, 17, 1160, and references cited therein); polynucleotides (DeClercq, P. F. et al. Biochem. Biophys. Res. Commun. 1975, 67, 255); pimaric acids (Glovsky, M. M. et al. J. Immunol. 1969, 102, 1); porphines (Lapidus, M. and Tomasco, J. Immunopharmacol. 1981, 3, 137); several antiinflammatories (Burge, J. J. et al. J. Immunol. 1978, 120, 1625); phenols (Muller-Eberhard, H. J. 1978, in Molecular Basis of Biological Degradative Processes, Berlin, R. D. et al., eds. Academic Press, New York, p. 65); and benzamidines (Vogt, W. et al Immunology 1979, 36, 138). Some of these agents function by general inhibition of proteases and esterases. Others are not specific to any particular intermediate step in the complement pathway, but, rather, inhibit more than one step of complement activation. Examples of the latter compounds include the benzamidines, which block C1, C4 and C5 utilization (see, e.g., Vogt et al. Immunol. 1979, 36, 138).
  • Additional agents known in the art that can inhibit activity of complement components include K-76, a fungal metabolite from Stachybotrys (Corey et al., J. Amer. Chem. Soc. 104: 5551, 1982). Both K-76 and K-76 COOH have been shown to inhibit complement mainly at the C5 step (Hong et al., J. Immunol. 122: 2418, 1979; Miyazaki et al., Microbiol. Immunol. 24: 1091, 1980), and to prevent the generation of a chemotactic factor from normal human complement (Bumpers et al., Lab. Clinc. Med. 102: 421, 1983). At high concentrations of K-76 or K-76 COOH, some inhibition of the reactions of C2, C3, C6, C7, and C9 with their respective preceding intermediaries is exhibited. K-76 or K-76 COOH has also been reported to inhibit the C3b inactivator system of complement (Hong et al., J. Immunol. 127: 104-108, 1981). Other suitable agents for practicing methods of the present invention include griseofulvin (Weinberg, in Principles of Medicinal Chemistry, 2d Ed., Foye, W. O., ed., Lea & Febiger, Philadelphia, Pa., p. 813, 1981), isopannarin (Djura et al., Aust. J. Chem. 36: 1057, 1983), and metabolites of Siphonodictyon coralli-phagum (Sullivan et al., Tetrahedron 37: 979, 1981).
  • A combination therapy regimen may be additive, or it may produce synergistic results (e.g., reductions in complement pathway activity more than expected for the combined use of the two agents). In some embodiments, the present invention provide a combination therapy for preventing and/or treating AMD or another complement related disease as described above with a C5-binding antibody of the invention and an anti-angiogenic, such as anti-VEGF agent.
    • 5.5. Diagnostic Uses
  • In one aspect, the invention encompasses diagnostic assays for determining C5 protein and/or nucleic acid expression as well as C5 protein function, in the context of a biological sample (e.g., blood, serum, cells, tissue) or from individual is afflicted with a disease or disorder, or is at risk of developing a disorder associated with AMD.
  • Diagnostic assays, such as competitive assays rely on the ability of a labelled analogue (the “tracer”) to compete with the test sample analyte for a limited number of binding sites on a common binding partner. The binding partner generally is insolubilized before or after the competition and then the tracer and analyte bound to the binding partner are separated from the unbound tracer and analyte. This separation is accomplished by decanting (where the binding partner was preinsolubilized) or by centrifuging (where the binding partner was precipitated after the competitive reaction). The amount of test sample analyte is inversely proportional to the amount of bound tracer as measured by the amount of marker substance. Dose-response curves with known amounts of analyte are prepared and compared with the test results in order to quantitatively determine the amount of analyte present in the test sample. These assays are called ELISA systems when enzymes are used as the detectable markers. In an assay of this form, competitive binding between antibodies and C5-binding antibodies results in the bound C5 protein, preferably the C5 epitopes of the invention, being a measure of antibodies in the serum sample, most particularly, neutralising antibodies in the serum sample.
  • A significant advantage of the assay is that measurement is made of neutralising antibodies directly (i.e., those which interfere with binding of C5 protein, specifically, epitopes). Such an assay, particularly in the form of an ELISA test has considerable applications in the clinical environment and in routine blood screening.
  • Immunologic techniques employ polyclonal or monoclonal antibodies against the different epitopes of the various complement components (e.g., C3, C4, C5) to detect, e.g., the split products of complement components (see, e.g., Hugli et al., Immunoassays Clinical Laboratory Techniques 443-460, 1980; Gorski et al., J Immunol Meth 47: 61-73, 1981; Linder et al., J Immunol Meth 47: 49-59, 1981; and Burger et al., J Immunol 141: 553-558, 1988). Binding of the antibody with the split product in competition with a known concentration of labeled split product could then be measured. Various assays such as radio-immunoassays, ELISA's, and radial diffusion assays are available to detect complement split products.
  • The immunologic techniques provide high sensitivity to detect complement activation, since they allow measurement of split-product formation in blood from a test subject and control subjects with or without macular degeneration-related disorders. Accordingly, in some methods of the present invention, diagnosis of a disorder associated with ocular disorders is obtained by measurement of abnormal complement activation through quantification of the soluble split products of complement components in blood plasma from a test subject. The measurements can be performed as described, e.g., in Chenoweth et al., N Engl J Med 304: 497-502, 1981; and Bhakdi et al., Biochim Biophys Acta 737: 343-372, 1983. Preferably, only the complement activation formed in vivo is measured. This can be accomplished by collecting a biological sample from the subject (e.g., serum) in medium containing inhibitors of the complement system, and subsequently measuring complement activation (e.g., quantification of the split products) in the sample.
  • In the clinical diagnosis or monitoring of patients with disorders associated with ocular diseases or disorders, the detection of complement proteins in comparison to the levels in a corresponding biological sample from a normal subject is indicative of a patient with disorders associated with macular degeneration.
  • In vivo diagnostic or imaging is described in US2006/0067935. Briefly, these methods generally comprise administering or introducing to a patient a diagnostically effective amount of a C5 binding molecule that is operatively attached to a marker or label that is detectable by non-invasive methods. The antibody-marker conjugate is allowed sufficient time to localize and bind to complement proteins within the eye. The patient is then exposed to a detection device to identify the detectable marker, thus forming an image of the location of the C5 binding molecules in the eye of a patient. The presence of C5 binding antibody or an antigen-binding fragment thereof is detected by determining whether an antibody-marker binds to a component of the eye. Detection of an increased level in selected complement proteins or a combination of protein in comparison to a normal individual without AMD disease is indicative of a predisposition for and/or on set of disorders associated with macular degeneration. These aspects of the invention are also preferred for use in eye imaging methods and combined angiogenic diagnostic and treatment methods.
  • The invention also pertains to the field of predictive medicine in which diagnostic assays, prognostic assays, pharmacogenomics, and monitoring clinical trials are used for prognostic (predictive) purposes to thereby treat an individual prophylactically.
  • The invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with dysregulation of complement pathway activity. For example, mutations in a C5 gene can be assayed in a biological sample. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with C5 protein, nucleic acid expression or activity.
  • Another aspect of the invention provides methods for determining C5 nucleic acid expression or C5 protein activity in an individual to thereby select appropriate therapeutic or prophylactic agents for that individual (referred to herein as “pharmacogenomics”). Pharmacogenomics allows for the selection of agents (e.g., drugs) for therapeutic or prophylactic treatment of an individual based on the genotype of the individual (e.g., the genotype of the individual examined to determine the ability of the individual to respond to a particular agent.)
  • Yet another aspect of the invention pertains to monitoring the influence of agents (e.g., drugs) on the expression or activity of C5 protein in clinical trials.
    • 5.6. Pharmaceutical Compositions
  • The invention provides pharmaceutical compositions comprising the C5-binding antibodies (intact or binding fragments) formulated together with a pharmaceutically acceptable carrier. The compositions can additionally contain one or more other therapeutica agents that are suitable for treating or preventing a complement-associated disease (e.g., AMD). Pharmaceutically carriers enhance or stabilize the composition, or to facilitate preparation of the composition. Pharmaceutically acceptable carriers include solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.
  • A pharmaceutical composition of the present invention can be administered by a variety of methods known in the art. The route and/or mode of administration vary depending upon the desired results. It is preferred that administration be intravenous, intramuscular, intraperitoneal, or subcutaneous, or administered proximal to the site of the target. In a specific embodiment, the antibodies of the invention are formulated so that they can be administered intravitreally into the eye. The pharmaceutically acceptable carrier should be suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound, i.e., antibody, bispecific and multispecific molecule, may be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound.
  • The composition should be sterile and fluid. Proper fluidity can be maintained, for example, by use of coating such as lecithin, by maintenance of required particle size in the case of dispersion and by use of surfactants. In many cases, it is preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol or sorbitol, and sodium chloride in the composition. Long-term absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.
  • Pharmaceutical compositions of the invention can be prepared in accordance with methods well known and routinely practiced in the art. See, e.g., Remington: The Science and Practice of Pharmacy, Mack Publishing Co., 20th ed., 2000; and Sustained and Controlled Release Drug Delivery Systems, J. R. Robinson, ed., Marcel Dekker, Inc., New York, 1978. Pharmaceutical compositions are preferably manufactured under GMP conditions. Typically, a therapeutically effective dose or efficacious dose of the C5-binding antibody is employed in the pharmaceutical compositions of the invention. The C5-binding antibodies are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art. Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present invention can be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. The selected dosage level depends upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors.
  • A physician or veterinarian can start doses of the antibodies of the invention employed in the pharmaceutical composition at levels lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved. In general, effective doses of the compositions of the present invention, for the treatment of an allergic inflammatory disorder described herein vary depending upon many different factors, including means of administration, target site, physiological state of the patient, whether the patient is human or an animal, other medications administered, and whether treatment is prophylactic or therapeutic. Treatment dosages need to be titrated to optimize safety and efficacy. For systemic administration with an antibody, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 15 mg/kg, of the host body weight. An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months. For intravitreal administration with an antibody, the dosage ranges from about 0.0001 to about 10 mg. An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months.
  • Antibody is usually administered on multiple occasions. Intervals between single dosages can be weekly, monthly or yearly. Intervals can also be irregular as indicated by measuring blood levels of C5-binding antibody in the patient. In some methods of systemic administration, dosage is adjusted to achieve a plasma antibody concentration of 1-1000 μg/ml and in some methods 25-500 μg/ml. Alternatively, antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, humanized antibodies show longer half life than that of chimeric antibodies and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime.
    • 6. EXAMPLES
  • The following examples are provided to further illustrate the invention but not to limit its scope. Other variants of the invention will be readily apparent to one of ordinary skill in the art and are encompassed by the appended claims.
    • Example 1 Generation of Cynomolgus C5 and Human C5 1. Generation of Cynomolgus C5
  • Cynomolgus C5 was purified successfully from cynomolgus serum by affinity chromatography using MOR07086 hu IgG1. Cynomolgus C5 was quality tested by SDS-PAGE, Western blot, mass spectrometry and hemolytic assays. Quality of purified cynomolgus C5 was shown to be high by SDS-PAGE and Western blot. Lack of C3 contamination was confirmed by SDS and Western blot. In addition, the identity of cynomolgus C5 sequence was determined by mass spectrometric analysis and the activity of purified cynomolgus C5 was tested in hemolytic assays. In hemolytic assays the new preparation was equipotent to human C5 (e.g., Sample 6, which was used in affinity maturation pannings, reconstituted complement activity of 20% human C5-depleted serum with similar activity to purified human C5).
    • 2. Quality Control of Human and Cynomolgus Biotinylated and Non-Biotinylated C5 Proteins
  • Bioactivity of purified human C5 was characterized and confirmed by the alternative pathway hemolytic activity. C5 was spiked into C5-depleted human serum at varying concentrations to obtain an EC50. EC50 values ranging between 0.02-0.1 nM were considered acceptable.
  • Before using, the bioactivity of every purified human C5 protein lot was tested in the hemolytic assay. The same quality control was done for cynomolgus C5 after purification from cynomolgus serum. After biotinylation of human and cynomolgus C5, the bioactivity of the material was also tested in hemolytic assays, in order to analyze if there was a loss of activity caused by biotinylation.
    • Example 2 Generation of C5-Specific Antibodies from the HuCAL GOLD® Library
  • C5 antibodies were generated by selection of clones having high binding affinities, using as the source of antibody variant proteins a commercially available phage display library, the MorphoSys HuCAL GOLD® library.
  • HuCAL GOLD® library is a Fab library (Knappik et al., 2000) in which all six CDRs are diversified by appropriate mutation, and which employs the CysDisplay™ technology for linking the Fab to the phage surface (WO01/05950, Löhning et al., 2001).
  • 1. Selection by Panning of C5 Specific Antibodies from the Library
  • For the selection of antibodies against C5, two different panning strategies were applied. The six different pools were individually subjected to three rounds of: (a) a solid phase panning where the antigens (human and cynomolgus C5) were directly coated on Maxisorp 96 well microtiter plates (Nunc, Wiesbaden, Germany); or (b) a solution panning with biotinylated human and cyno C5 where the phage-antigen complex was captured by Streptavidin magnetic beads (Dynabeads M-280; Dynal) for each panning pool.
  • The HuCAL GOLD® library was amplified in 2×YT medium containing 34 μg/ml chloramphenicol and 1% glucose (2×YT-CG). After infection with VCSM13 helper phage at an OD600 nm of 0.5 (30 min at 37° C. without shaking; 30 min at 37° C. shaking at 250 rpm), cells were spun down (4120 g; 5 min; 4° C.), resuspended in 2×YT/34 μg/ml chloramphenicol/50 μg/ml kanamycin/0.25 mM IPTG and grown overnight at 22° C. Phage were PEG-precipitated from the supernatant, resuspended in PBS/20% glycerol and stored at −80° C. Phage amplification between two panning rounds was conducted as follows: mid-log phase E. coli TG1 cells were infected with eluted phage and plated onto LB-agar supplemented with 1% of glucose and 34 μg/ml of chloramphenicol (LB-CG plates). After overnight incubation at 30° C., the TG1 colonies were scraped off the agar plates and used to inoculate 2×YT-CG until an OD600 nm of 0.5 was reached. VCSM13 helper phage were added for infection as described above.
  • Taken together 354 clones derived from all panning strategies were sequenced, resulting in 64 unique clones with the desired profile: binding to human and cynomolgus C5 and no binding to the counter targets C3 and C4.
  • 45 clones derived from solid phase pannings and 19 clones from solution pannings were selected for protein expression and purification. Four Fabs from solid phase pannings (MOR06525, MOR06756, MOR06757 and MOR06763) and 6 Fabs from solution pannings (MOR07086, MOR07087, MOR07091, MOR07092, MOR07093 and MOR07094) entered affinity maturation.
    • Solid Phase Panning Against C5 on Directly Coated Protein
  • The first panning variant was solid phase panning alternating human C5 (first and third round of selection) and cynomolgus C5 (second round of selection).
  • Three wells of a Maxisorp plate (F96 Nunc-Immunoplate) were coated with 200 μl of 50 nM C5 each o/n at 4° C. The coated wells were washed 2× with 400 μl PBS and blocked with 350 μl 5% MPBS for 2 h at RT on a microtiter plate shaker. For each panning about 1013 HuCAL GOLD® phage-antibodies were blocked with equal volume of PBST/5% milk powder for 2 h at room temperature. The coated wells were washed 2× with 400 μl PBS after the blocking procedure. 200 μl of pre-blocked HuCAL GOLD® phage-antibodies were added to each coated well and incubated for 2 h at RT on a shaker. Washing was performed by adding five times 350 μl PBS/0.05% Tween, followed by washing another five times with PBS. For some panning conditions a more stringent wash procedure was applied.
  • Elution of phage from the plate was performed with 200 μl 20 mM DTT in 10 mM Tris/HCl pH8 per well for 10 min. The DTT phage eluate was added to 15 ml of E. coli TG1, which were grown to an OD600 of 0.6-0.8 at 37° C. in 2YT medium and incubated in 50 ml plastic tubes for 45 min at 37° C. without shaking for phage infection. After centrifugation for 5 min at 4120×g, the bacterial pellets were each resuspended in 600 μl 2×YT medium, plated on 3×YT-CG agar plates and incubated overnight at 37° C. Colonies were scraped off the plates and phages were rescued and amplified as described above.
  • The second and third rounds of solid phase panning were performed according to the protocol of the first round. In the second selection round for some panning conditions the output of the first round was used for selections on cynomolgus C5 in order to enrich for cynomolgus cross-reactive antibodies.
  • For some panning conditions washing stringency was increased and antigen concentration was decreased within the three round of selection in order to generate high affinity antibodies.
  • The HuCAL GOLD® phagemid library was used to select specific Fab antibody fragments against human C5. First strategy was a solid phase panning on directly coated human C5 protein (panning procedure described above).
  • After the 3rd panning round, the enriched phage pools were subcloned from the pMORPH®23 library vector (allowing efficient antibody display on the phage surface) into the pMORPH®×9_Fab_MH expression vector which mediates periplasmic expression of soluble Fabs. Single clones were picked and soluble Fabs were expressed from these single clones.
  • In total, 6624 clones were analyzed in primary screening which was performed by binding of the Fabs directly from the bacterial lysates to human C5 immobilized on Maxisorp microtiter plates. 1660 hits were obtained from the primary screening on human C5 with signals >5-fold over background. 384 hits were further analyzed in a secondary screening to confirm binding on human C5 and to screen for binding to the counter targets human C3 and C4.
  • Many primary hits could be confirmed on human C5 and showed no cross-reactivity to human C3 and C4, but only 6 Fabs had weak cross-reactivity to cynomolgus C5.
  • As a first consequence new solid phase pannings were performed alternating on human and cynomolgus C5. In parallel, quality controls of the purified cynomolgus C5 batch revealed a high amount of cynomolgus C3 within the cynomolgus C5 batch. Considering this results, a new method to screen for cynomolgus cross-reactive antibodies was applied. Cynomolgus C5 was captured from cynomolgus serum using an C5-binding polyclonal antibody (see Example 3, section 3). Using this method the initial primary hits were screened again on cynomolgus C5 and 56 clones were confirmed for binding to cynomolgus C5.
  • For the alternating solid phase pannings, the 1st round output of the most successful 12 human solid phase pannings was used for selections on cynomolgus C5 (protein batch contaminated with cynomolgus C3; not known during pannings). 376 clones were confirmed in a secondary screening for binding to human C5 and 361 clones for binding to cynomolgus C5 captured from cynomolgus serum.
    • Solution Panning on Biotinylated C5 Protein
  • The second panning variant was solution panning against biologically active (in hemolytic assays) biotinylated human C5 and biotinylated cynomolgus C5.
  • For this panning 200 μl of Streptavidin magnetic beads (Dynabeads M-280; Dynal) were washed once with PBS and blocked with Chemiblocker for 2 h at RT. 300 μl of the PBS diluted phage were blocked also with Chemiblocker for 1-2 h at RT on a rotator. The blocked phages were twice pre-adsorbed against 50 μl blocked Streptavidin magnetic beads for 30 min. The phage supernatant was transferred to a new blocked 2 ml reaction tube and human biotinylated C5 was added and incubated for 1 h at RT on a rotator. 100 μl of the blocked Streptavidin magnetic beads were added to each panning pool an incubated for 10 min on a rotator. The beads were collected with a particle separator (Dynal MPC-E) for approx. 2.5 min and the solution was removed carefully.
  • Beads were then washed 7× in PBS/0.05% Tween using a rotator, followed by washing another three times with PBS. Elution of phage from the Dynabeads was performed by adding 200 μl 20 mM DTT in 10 mM Tris/HCl pH 8 to each tube and incubation for 10 min. Dynabeads were removed by the magnetic particle separator and the supernatant was added to 15 ml of an E. coli TG-1 culture grown to OD600 nm of 0.6-0.8. Beads were then washed once with 200 μl PBS and together with additionally removed phages the PBS was added to the 15 ml E. coli TG-1 culture. For phage infection, the culture was incubated in 50 ml plastic tubes for 45 min at 37° C. without shaking. After centrifugation for 5 min at 4120×g, the bacterial pellets were resuspended each in 600 μl 2×YT medium, plated on 3×YT-CG agar plates and incubated overnight at 37° C. Colonies were scraped off the plates and phages were rescued and amplified as described above. The second and third rounds of selection were performed in an identical way to the first round of selection.
  • A further panning strategy was solution panning using human C5 and alternating human and cynomolgus C5 (protein batch contaminated with cynomolgus C3, not known during pannings). Therefore the proteins were biotinylated and the retained bio-functionality after the biotinylation procedure was confirmed in hemolytic bioassays.
  • The phage-antigen complex was captured on Streptavidin magnetic beads via the biotin moiety of the antigen. After washing only specific bound phage were eluted (panning procedure described above).
  • First screening was done on directly coated proteins (see Example 3, section 1) and only 80 clones could be confirmed on human C5. Due to the fact that during the pannings the antigen was kept in solution, a new screening method was developed. In a solution ELISA the Fabs were incubated with biotinylated antigen on a NeutrAvidin plate. Using this solution screening procedure, a significantly higher amount of human and cynomolgus C5 specific clones could be selected. These results confirmed that many Fabs derived from solution pannings recognize C5 only in solution or when captured (e.g. via a polyclonal C5-binding antibody).
    • 2. Subcloning and Expression of Selected Fab Fragments
  • To facilitate rapid expression of soluble Fabs, the Fab-encoding inserts of the selected HuCAL GOLD® phages were subcloned via XbaI and EcoRI into the E. coli expression vector pMORPH®x9_MH. Fab fragments carry a C-terminal Myc tag and as a second C-terminal tag the 6× His-tag (Chen et al., Gene 139:73-75 (1994)). After transformation of the expression plasmids into E. coli TG1 F-cells chloramphenicol-resistant single clones were picked into the wells of a sterile 384-well microtiter plate pre-filled with 60 μl 2×YT-CG medium and grown o/n at 30° C. 5 μl of each E. coli TG-1 o/n culture were transferred to a fresh, sterile 96-well microtiter plate pre-filled with 40 μl 2×YT medium supplemented with 34 μg/ml chloramphenicol per well. The microtiter plates were incubated at 30° C. shaking at 400 rpm on a microplate shaker until the cultures were slightly turbid (˜2-4 h) with an OD600 nm of ˜0.5. To these expression plates, 10 μl 2×YT medium supplemented with 34μ/ml chloramphenicol and 3 mM IPTG (isopropyl-β-D-thiogalactopyranoside) was added per well (end concentration 0.5 mM IPTG). The microtiter plates were sealed with a gas-permeable tape, and incubated o/n at 30° C. shaking at 400 rpm. To each well of the expression plates, 15 μl BEL buffer was added containing 2.5 mg/ml lysozyme, 4 mM EDTA and 10 U/μl Benzonase and incubated for 1 h at 22° C. on a microtiter plate shaker (400 rpm) followed by an optional freezing step for at least 2 h at −80° C. The BEL extracts were used for binding analysis by ELISA or Fab SET screening after affinity maturation.
  • Expression of Fab fragments encoded by pMORPH® x9_Fab_MH in TG-1 cells was carried out in shaker flask cultures using 750 ml of 2×YT medium supplemented with 34 μg/ml chloramphenicol. Cultures were shaken at 30° C. until the OD600 nm reached 0.5. Expression was induced by addition of 0.75 mM IPTG for 20 h at 30° C. Cells were disrupted using lysozyme and Fab fragments isolated by Ni-NTA chromatography (Qiagen, Hilden, Germany). Buffer exchange to 1× Dulbecco's PBS (pH 7.2) was performed using PD10 columns. Samples were filtered sterile (0.2 μm, Millipore). Purity of samples was determined in denatured, reduced state by SDS-PAGE (15% Criterion Gels, BioRad) and in native state by size exclusion chromatography (HP-SEC). Protein concentrations were determined by UV-spectrophotometry (Krebs et al., J. Immunol. Methods 254:67-84 (2001)).
  • On Fab level, the overall expression rates and the percentage of monomeric fraction in SEC (Size Exclusion Chromatography) ranged from acceptable to good for most of the identified antibody fragments. 64 parental Fabs were expressed and 61 Fabs could be purified. 60 affinity matured Fabs were purified in the mg scale. Most of the Fabs were good expressors and had no aggregation tendency.
    • Example 3 Identification of C5-Specific Antibodies from the HuCAL GOLD® Library
  • Below four different Enzyme Linked Immunosorbent Assay (ELISA) methods describe the screening of C5-binding antibodies (as bacterial BEL lysates or purified Fabs) on specific and counter antigens.
    • 1. Screening on Directly Coated Protein
  • Maxisorp (Nunc, Rochester, N.Y., USA) 384 well plates were coated with 20 μl per well of 2.5 μg/ml antigen (human C5 and the counter proteins human C3 and C4) in PBS, pH 7.4 o/n at 4° C. In parallel, plates were coated with 20 μl per well of 5 μg/ml sheep anti-human IgG, Fd fragment specific (The Binding Site, Birmingham, UK), diluted in PBS, pH 7.4 to check for Fab expression level.
  • The plates were blocked with PBS/0.05% Tween 20 (PBST) containing 5% milk powder for 1-2 h at RT. After washing the wells with PBST, BEL-extracts, purified HuCAL GOLD® Fabs or control Fabs diluted in PBS were added and incubated for 1 h at RT. To detect Fab binding, anti-HIS6 antibody coupled to peroxidase was applied (Roche).
  • For detection of POD-conjugates fluorogenic substrate QuantaBlu (Pierce) was used according to manufacturer's instructions. Between all incubation steps, the wells of the microtiter plates were washed three times and five times with PBST after the final incubation with the secondary antibody. Fluorescence was measured in a Tecan GENios Pro plate reader.
  • 2. Solution Screening with Biotinylated Proteins
  • The ELISA method described below was used for screening of HuCAL GOLD® Fabs after solution pannings using biotinylated complement proteins.
  • NeutrAvidin plates were blocked with 1× Chemiblocker (Chemicon) diluted in PBS o/n at 4° C. These plates were used to screen for binding to human C5 and to the counter targets C3 and C4. In parallel, Maxisorp 384 well plates (Nunc, Rochester, N.Y., USA) were coated with 20 μl per well of 5 μg/ml sheep anti-human IgG, Fd fragment specific (The Binding Site, Birmingham, UK), diluted in PBS, pH 7.4. These plates were used to check for Fab expression levels and for non-specific biotin binding. On the next day, coated Maxisorp plates were washed 2× with PBST and blocked with 3% BSA in TBS for 1-2 h at RT. Periplasmic BEL extracts containing Fabs or purified HuCAL GOLD® Fabs were added to both blocked NeutrAvidin and Maxisorp plates.
  • Subsequently, 20 μl per well of biotinylated human C5 (to detect specific binding) and in parallel, biotinylated human C3 and C4 (to detect unwanted binding) were added to wells of the NeutrAvidin plates. The biotinylated antigens were incubated with the HuCAL GOLD® Fabs for 1-2 h at RT. Biotinylated unrelated antigen Transferrin was then added to the Maxisorp plates to check for biotin binding Fabs (in this case the HuCAL®-Fab fragments were previously captured via anti-Fd antibody).
  • Following secondary antibodies were applied for detection: Alkaline phosphatase (AP)-conjugated Streptavidin-AP AffiniPure F(ab′)2 fragment, goat anti-human, was added to the Maxisorp expression plates; anti-HIS6 Peroxidase conjugated mouse antibody, Roche, was added to the NeutrAvidin plates and Streptavidin-Alkaline Phosphatase, ZYMED, was added to the Maxisorp plates with the biotinylated Transferrin.
  • For detection of AP-conjugates, fluorogenic substrate AttoPhos (Roche Diagnostics, Mannheim, Germany) and for detection of POD-conjugates, fluorogenic substrate QuantaBlu (Pierce) were used according to manufacturer's instructions. Fluorescence was measured in a Tecan GENios Pro plate reader.
  • Using this method it was possible to screen for anti-human C5 Fabs which recognize human C5 in solution and to exclude antibodies binding to the biotin moiety of the target antigens.
    • 3. Determination of Cross-Reactivity to Cynomolgus C5
  • A polyclonal C5-binding antibody (US Biological Cat#C7850-24) was used to capture cynomolgus C5 from cynomolgus serum.
  • 384 well Maxisorp plates were coated with 20 μl/well of 5 μg/ml polyclonal C5-binding in PBS and incubated o/n at 4° C. On the next day the plates were washed 3× with PBST and blocked with 100 μl/well of diluent (4% BSN 0.1% Tween20/0.1% Triton-X 100/PBS) for 2 hours at RT. Cynomolgus serum was diluted 1:20 in diluent (4% BSN 0.1% Tween20/0.1% Triton-X 100/PBS) (˜approx. concentration of cynomolgus C5 4 μg/ml) and 20 μl/well was added to the 2×PBST washed Maxisorp plates. After 1 h incubation at RT the plates were washed 3×PBST and BEL lysates containing Fab fragments or purified Fabs were added and incubated for 1 h at RT. The plates were washed again and detection antibody anti-HIS6-POD (Roche #1965085), was added. POD substrate, BM Blue, soluble, (Roche Applied Science) was added and the reaction was stopped with 1M H2SO4. Absorbance was read at 450 nm using the BMG Reader device.
    • Example 4 Affinity Maturation 1. Construction of Affinity Maturation Libraries of Selected C5-Binding Fabs
  • To increase affinity and biological activity of selected antibody fragments, L-CDR3 and H-CDR2 regions were optimized in parallel by cassette mutagenesis using trinucleotide directed mutagenesis (see e.g., Virnekas et al., Nucleic Acids Res. 22:5600-5607 (1994)), while the framework regions were kept constant. Prior to cloning for affinity maturation, all parental Fab fragments were transferred from the corresponding expression vector (pMORPH®x9_MH) into the CysDisplay™ vector pMORPH®25 via XbaI/EcoRI. pMORPH®25 was created from the HuCAL GOLD® display vector pMORPH®23 by removal of one BssHII site interfering with library cloning for H-CDR2 optimization. For optimizing L-CDR3 of parental Fabs, the L-CDR3, framework 4 and the constant region of the light chains (405 bp) of the binders were removed by Bpil/Sphl and replaced by a repertoire of diversified L-CDR3s together with framework 4 and the constant domain.
  • 10 parental C5-binding Fabs were divided in 7 pools according to different selection criteria and only Fabs with same framework were put together: (1) MOR07086; (2) MOR06525+6756 (same framework); (3) MOR06757; (4) MOR06763; (5) MOR07087; (6) MOR07091+7092 (same framework); (7) MOR07093+7094 (same framework).
  • Approximately 1.5 μg of the single Fab vector fragment and of the Fab pool were ligated with a 3 to 5-fold molar excess of the insert fragment carrying the diversified L-CDR3s. In a second library set, the H-CDR2 (Xhol/BssHII) was diversified while the connecting framework regions were kept constant. In order to monitor the cloning efficiency, the parental H-CDR2 was replaced by a dummy before the diversified H-CDR2 cassette was cloned in.
  • Ligation mixtures of the different libraries were electroporated into E. coli TOP10 F′ cells (Invitrogen) yielding from 2×107 to 2×108 independent colonies. The libraries were amplified. For quality control, several single clones per library were randomly picked and sequenced using primers CFR84 (VL) and OCAL_Seq_Hp (VH).
  • As described above, seven maturation sub pools were generated and kept separate during the subsequent selection process.
  • 14 different affinity maturation libraries (one LCDR3 and one HCDR3 library for each lead or pool) were generated by standard cloning procedures and transformation of the diversified clones into electro-competent E. coli TOP10F′ cells (Invitrogen). Library sizes were good, being in the range of 2×107-5×108. Sequencing of randomly picked clones showed a diversity of 100%. No parental binders but derivatives of all respective parental input binders were found. Finally phages of all 14 libraries were prepared separately.
  • TABLE 2
    Overview of maturation libraries
    MOR0 Maturation VH/VL Type Library Size
    6757 HCDR2 VH3 3.70 × 10E7
    6763 HCDR2 VH3 4.95 × 10E7
    7086 HCDR2 VH1A 1.58 × 10E8
    7087 HCDR2 VH1A 7.85 × 10E7
    6525 + 6756 HCDR2 VH5 5.22 × 10E7
    7091 + 7092 HCDR2 VH5 3.51 × 10E7
    7093 + 7094 HCDR2 VH2 2.01 × 10E7
    6757 LCDR3 Vkappa1 1.89 × 10E7
    6763 LCDR3 Vlambda2 7.35 × 10E7
    7086 LCDR3 Vlambda3 7.54 × 10E7
    7087 LCDR3 Vkappa1 5.46 × 10E7
    6525 + 6756 LCDR3 Vlambda2 8.50 × 10E7
    7091 + 7092 LCDR3 Vlambda3 4.93 × 10E8
    7093 + 7094 LCDR3 Vlambda2 1.33 × 10E8
    • 2. Preparation of Antibody-Phages for Affinity Maturation
  • The HuCAL® maturation libraries were amplified in 2×YT medium containing 34 μg/ml chloramphenicol and 1% glucose (2×YT-CG). After infection with VCSM13 helper phage at an OD600 nm of 0.5 (30 min at 37° C. without shaking; 30 min at 37° C. shaking at 250 rpm), cells were spun down (4120×g; 5 min; 4° C.), resuspended in 2×YT/34 μg/ml chloramphenicol/50 μg/ml kanamycin/0.25 mM IPTG and grown o/n at 22° C. Phages were PEG-precipitated twice from the supernatant, resuspended in PBS and used for the maturation pannings described below.
    • 3. Standard Solution Maturation Panning on Biotinylated C5 Protein
  • About 1012 phages rescued from the generated affinity maturation libraries, as described above, were subjected to pannings performed under very stringent conditions to select for affinity improved C5 specific Fabs.
  • Solution pannings using the respective phage pools were either performed using biotinylated human C5 or alternating biotinylated human and cynomolgus C5 proteins. In order to increase panning stringency and to select for improved off-rates, antigen concentration was decreased and prolonged washing periods were applied (washing conditions are listed in Table 3).
  • TABLE 3
    Increased washing conditions within the selection rounds of
    solution maturation pannings
    Selection Rd. Washing conditions (modified: stringent)
    1st round 4x PBS/0.05% Tween 5 min on rotator
    3x PBS/0.05% Tween 15 min on rotator-> transfer
    magnetic beads with the captured antigen and phages
    to a fresh blocked tube
    4x PBS quick
    3x PBS
    5 min on rotator-> transfer magnetic
    beads with the captured antigen and phages to a
    fresh blocked tube
    2nd round 3x PBS/0.05% Tween quick
    7x PBS/0.05% Tween 15 min on rotator-> transfer
    magnetic beads with the captured antigen and phages
    to a fresh blocked tube
    3x PBS quick
    7x PBS 15 min on rotator-> transfer magnetic
    beads with the captured antigen and phages to a
    fresh blocked tube
    3rd round 5x PBS/0.05% Tween quick
    8x PBS/0.05% Tween 15 min on rotator
    1x PBS/0.05% Tween o/n on rotator
    3x PBS/0.05% Tween quick
    6x PBS/0.05% Tween 15 min on rotator -> transfer
    magnetic beads with the captured antigen and phages
    to a fresh blocked tube
    5x PBS quick
    8x PBS 15 min on rotator -> transfer magnetic
    beads with the captured antigen and phages to a
    fresh blocked tube
  • Pre-blocked phage (1:2 mixture with 2× Chemiblocker incubated for 1 h at RT) were incubated with low concentration of biotinylated C5 protein for 1-2 h at RT. The panning strategy is similar to a standard solution panning described above. The phage antigen complex was captured via the biotin moiety of C5 to pre-blocked Streptavidin magnetic beads 30 min at RT. Beads were then washed more stringently compared to a normal panning. Elution and amplification of phage was performed as described above.
  • The second and third rounds of selection were performed in an identical way to the first round, but at higher stringency washing conditions and lower antigen concentrations. For each antibody lead or pool several different pannings were performed. For each panning strategy different stringency conditions were applied. Panning strategies are summarized in Table 4.
  • TABLE 4
    Overview of solution maturation pannings 1783 and 1784 on biotinylated human C5
    and biotinylated cynomolgus C5
    Panning Panning Antigen Antigen Antigen
    # Library mode 1st round 2nd round 3rd round Antigen Conc. Washing
    1783.1 MOR06525 + 6756 HCDR2 solution human C5 human C5 human C5   50 nM human/ modified
    1783.2 MOR07086 HCDR2 Streptavidin   5 nM human/ (more
    1783.3 MOR06763 HCDR2 beads 0.25 nM human stringent)
    1783.4 MOR07087 HCDR2
    1783.5 MOR06525 + 6756 LCDR3
    1783.6 MOR07086 LCDR3
    1783.7 MOR06763 LCDR3
    1783.8 MOR07087 LCDR3
    1783.9 MOR06525 + 6756 HCDR2 solution human C5 cyno C5 human C5   25 nM human/ modified
    1783.10 MOR07086 HCDR2 Streptavidin   5 nM cyno/ (more
    1783.11 MOR06763 HCDR2 beads 0.25 nM human stringent)
    1783.12 MOR06525 + 6756 LCDR3
    1783.13 MOR07086 LCDR3
    1783.14 MOR06763 LCDR3
    1784.1 MOR06757 HCDR2 solution human C5 human C5 human C5   50 nM human/ modified
    1784.2 MOR07091 + 7092 HCDR2 Streptavidin   5 nM human/ (more
    1784.3 MOR07093 + 7094 HCDR2 beads 0.25 nM human stringent)
    1784.4 MOR06757 LCDR3
    1784.5 MOR07091 + 7092 LCDR3
    1784.6 MOR07093 + 7094 LCDR3
    1784.7 MOR06757 HCDR2 solution human C5 cyno C5 human C5   25 nM human/ modified
    1784.8 MOR07091 + 7092 HCDR2 Streptavidin   5 nM cyno/ (more
    1784.9 MOR07093 + 7094 HCDR2 beads 0.25 nM human stringent)
    1784.10 MOR07087 HCDR2
    1784.11 MOR06757 LCDR3
    1784.12 MOR07091 + 7092 LCDR3
    1784.13 MOR07093 + 7094 LCDR3
    1784.14 MOR07087 LCDR3
  • After maturation pannings, the enriched phagemid pools were sub-cloned into pMORPH®x9_MH expression vector.
  • 4. Cross-Combination of Optimized VL (L-CDR3) with Optimized VH (H-CDR2)
  • For further improvement of affinity and potency, the independently optimized heavy and light chains from matured antibodies, derived from the same parental clone, were combined (see e.g., Rauchenberger et al., J. Biol. Chem. 278:38194-38205 (2003); Chen et al., J. Mol. Biol. 293:865-881 (1999); and Schier et al., J. Mol. Biol. 263:551-567 (1996)). This procedure, called cross-cloning, was applied for binders deriving from the same parental clones.
    • 5. Affinity Screening and Maturation Panning Outcome
  • A total of 2640 clones derived from all pannings were screened as bacterial lysates for improved affinities on human C5. Preliminary affinities were estimated by solution equilibrium titration (SET). Based on their estimated affinities, clones derived from each parental Fab or Fab pools were sequenced. Table 5 shows number of sequenced clones and number of obtained unique sequences for each panning condition.
  • TABLE 5
    Overview of affinity improved clones selected for sequence analysis
    Sequenced Unique
    Parental/Maturation Antigen clones Sequences Parental of unique
    MOR06525 + 6756 HCDR2 hu/hu/hu 10 9 6525
    MOR07086 HCDR2 hu/hu/hu 10 4 7086
    MOR06763 HCDR2 hu/hu/hu 22 10 6763(8x), 7086(2x)
    MOR07087 HCDR2 hu/hu/hu 10 4 7087
    MOR06757 HCDR2 hu/hu/hu 10 0
    MOR07091 + 7092 HCDR2 hu/hu/hu 24 7 7092
    MOR07093 + 7094 HCDR2 hu/hu/hu 10 10 7093
    MOR06525 + 6756 LCDR3 hu/hu/hu 20 5 6756
    MOR07086 LCDR3 hu/hu/hu 10 5 7086
    MOR06763 LCDR3 hu/hu/hu 10 8 7086
    MOR07087 LCDR3 hu/hu/hu 6 1 7086
    MOR06757 LCDR3 hu/hu/hu 16 0
    MOR07091 + 7092 LCDR3 hu/hu/hu 6 6 7091(1x), 7092(5x)
    MOR07093 + 7094 LCDR3 hu/hu/hu 10 9 7094
    MOR06525 + 6756 HCDR2 hu/cyno/hu 10 8 6525
    MOR07086 HCDR2 hu/cyno/hu 10 6 7086
    MOR06763 HCDR2 hu/cyno/hu 22 5 6763
    MOR06757 HCDR2 hu/cyno/hu 15 2 6757
    MOR07091 + 7092 HCDR2 hu/cyno/hu 15 6 7091(3x), 7092(3x)
    MOR07093 + 7094 HCDR2 hu/cyno/hu 10 10 7093
    MOR07087 HCDR2 hu/cyno/hu 10 6 7087(5x), 7086(1x)
    MOR06525 + 6756 LCDR3 hu/cyno/hu 12 0
    MOR07086 LCDR3 hu/cyno/hu 10 1 7086
    MOR06763 LCDR3 hu/cyno/hu 10 0
    MOR06757 LCDR3 hu/cyno/hu 9 1 7094
    MOR07091 + 7092 LCDR3 hu/cyno/hu 11 9 7091(6x), 7092(3x)
    MOR07093 + 7094 LCDR3 hu/cyno/hu 10 7 7094
    MOR07087 LCDR3 hu/cyno/hu 10 0
    Sum 338 139
    • 6. Sequence Analysis and Selection of Affinity Optimized Fabs for Protein Production
  • A very good diversity was maintained by recovering derivatives of all 10 parental Fabs. The nucleotide sequences of the heavy chain (VH) for 188 HCDR2 improved clones and the light chain (VL) variable regions for 150 improved LCDR3 clones were determined. 87 unique HCDR2 and 52 unique LCDR3 sequences were selected for a detailed analysis of sequence diversity within the matured CDRs. Fabs containing possible glycosylations sites in the CDRs were omitted from further characterizations.
  • The VH and VL sequence analysis and affinity data showed that all 10 parental Fabs yielded affinity-improved successors. Parental Fabs MOR06525, MOR06757, MOR06763, MOR07087 and MOR07094 yielded only HCDR2 improved clones and parentals MOR06756 and MOR07093 yielded only LCDR3 improved clones. MOR07086, MOR07091 and MOR07092 had matured clones for both VH and VL. This later allowed cross-cloning of VH and VL matured chains. From all data, 60 clones with best affinity and highest diversity in the matured CDRs were selected for Fab expression. Selected VH and VL amino acid, as well as nucleotide sequences, are listed in Table 1.
    • Example 5 IgG Conversion
  • 1. Conversion into Human IgG2 Format
  • In order to express full length immunoglobulin (Ig), variable domain fragments of heavy (VH) and light chains (VL) were subcloned from the pMORPH®x9_MH Fab expression vectors into pMORPH®2_h_Ig vector series for human IgG2. Restriction enzymes Mfel, and Blpl were used for subcloning of the VH domain fragment into pMORPH®2_h_IgG2. Subcloning of the VL domain fragment into pMORPH®2_h_Igκ was performed via the EcoRV and BsiWl sites, whereas subcloning into pMORPH®2_h_Igλ2 was done using EcoRV and Hpal.
  • All ten parental Fabs (MOR06525, 6756, 6757, 6763, 7086, 7087, MOR07091, 7092, 7093 and 7094) were converted into human IgG2. The IgGs were also expressed.
  • 2. Conversion into Human IgG1AA Format
  • In order to express full length immunoglobulin, variable domain fragments of Fab heavy (VH) and light chains (VL) were subcloned from the Fab expression vectors into IgG1 expression vectors. Restriction enzymes Mfel, and Blpl were used for subcloning of the VH domain fragment into pMORPH®2_h_IgG1AA, in which leucines at positions 234 and 235 were mutated to alanines to abrogate FcRγ binding and attenuate effector functions. The restrictions enzymes EcoRV and Hpal were used to subclone of the VL domain fragment into pMORPH®2_h_Igλ2.
  • Following matured Fabs with desired profile were subcloned into human IgG1AA format: MOR07832, 7834, 7872, 7876, 7829, 7871, 7865, 7873, 7830, 7878, 7910. Cross-cloning on IgG level was achieved by transfecting cells with combinations of light and heavy chain constructs. For example, MOR08114 was the product of the germlined heavy chain from MOR07829 and the germlined light chain from MOR07871. Table 6 summarizes the most relevant cross-cloned germlined IgGs.
  • TABLE 6
    Overview of most relevant cross-cloned germlined IgGs
    MOR0 VH/VL VH/VL matured CDRs
    Nr. germlined VH VL matured VH matured VL format
    8114 yes 7829 7871 7091/HCDR2 7091/LCDR3 hutg1AA
    8125 yes 7091 7873 — 7091/LCDR3 hutg1AA
    8126 yes 7829 7873 7091/HCDR2 7091/LCDR3 hutg1AA
    8127 yes 7830 7873 7091/HCDR2 7091/LCDR3 hutg1AA
    8128 yes 7092 7878 — 7092/LCDR3 hutg1AA
    8129 yes 7909 7092 7092/HCDR2 — hutg1AA
    8130 yes 7909 7878 7092/HCDR2 7092/LCDR3 hutg1AA
    8131 yes 7910 7092 7092/HCDR2 — hutg1AA
    8132 yes 7910 7878 7092/HCDR2 7092/LCDR3 hutg1AA
    • 3. Transient Expression and Purification of Human IgG
  • Eukaryotic HKB11 and HEK293 cells were transfected with an equimolar ratio of IgG heavy and light chain expression vector DNA. Cell culture supernatant was harvested at 3 or 7 days post transfection and subjected to standard protein A affinity chromatography (rProteinA FF or MabSelect SURE, GE Healthcare). As not otherwise stated, buffer exchange was performed to 1× Dulbcecco's PBS (pH 7.2, Invitrogen) and samples were sterile filtered (0.2 μm). Purity of IgG was analyzed under denaturing, reducing and non-reducing conditions in SDS-PAGE or by using Agilent BioAnalyzer and in native state by HP-SEC.
    • Example 6 Germlining
  • IgG constructs were germlined via site-directed mutagenesis using QuickChange® Site-Directed Mutagenesis Kit (Stratagene). The N-terminal DI of MOR08111 Vλ2 were changed to ES to match human germline sequence as well as to avoid a terminal Q (N-terminal Q can form pyroglutamine). N-terminal DI of MOR08110 Vλ3, MOR08113 Vλ3, and MOR08114 Vλ3 were germlined to SY, the most commonly found sequence in human λ3 genes. N-terminal QVQ of MOR08111 VH2 was germlined to EVT to match a λ2 gene and avoid terminal Q. N-terminal Q in MOR08109 VH5, MOR08110 VH5, MOR08113 VH5 and MOR08114 VH5 was also mutated to E.
  • Framework sequences for MOR08109 Vλ3 were synthesized to match the human λ3j gene and cloned into the expression vector using Nhel and Hpal restriction sites. Sequence alignments of the antibodies variable domains with their respective closest related human germline sequences are shown in FIG. 1.
    • Example 7 Affinity Determination 1. Kon/Koff and KD Determination of Anti-Human C5 Antibodies Using Surface Plasmon Resonance (Biacore)
  • It was determined that anti-Fab antibodies used to immobilize Fabs to the Biacore chip were influencing differently the binding affinity of each Fab for human C5, thus making the comparison of the Fabs to each other difficult. Biacore analysis was performed on IgG antibodies.
  • A CM4 chip was coated with 50 μg/ml goat anti-human Fc antibody (500-2000 RU) in 10 mM acetate buffer, pH 4.5, using standard EDC-NHS amine coupling chemistry. Each anti-human C5 IgG was captured on the chip in HBS-EP buffer at constant flow rate of 10 μl/min for a contact time leading to a ligand density around 20 RU. After capturing the anti-hu C5 IgG, different concentrations of human or cynomolgus C5, in the range between 0.156 nM to 2.5 nM, were injected. Each cycle was completed with two regeneration steps with phosphoric acid. All running conditions were carried out at 25° C. in 1×HBS-EP buffer. The resulting signals were adjusted by double referencing, substracting the refraction index values from the reference flow cell and the binding step with no analyte. Data were collected at 10 Hz and analyzed using the Biacore T100 Evaluation Software Version 1.1 (GE). This program uses a global fitting analysis method for the determination of rate and affinity constants for each interaction.
  • The specificity of the antibodies were measured. Preferably, the Kon and Koff values for binding to human and cynomolgus C5 are as follows: Kon >1×105, Koff <1×104). These measurements were performed in Biacore for the germlined IgGs and resulting data are listed in Table 7.
  • TABLE 7
    KD, Kon and Koff values of the germlined
    IgGs determined in Biacore
    antiC5 final IgG C5 sample ka [1/Ms] kd [1/s] KD [pM]
    MOR08109 huC5 2.13E+06 2.56E−05 12
    cynoC5 1.23E+06 4.49E−05 37
    MOR08110 huC5 4.15E+06 4.69E−05 12
    cynoC5 1.81E+06 9.24E−05 60
    MOR08111 huC5 1.00E+06 3.07E−05 31
    cynoC5 8.91E+05 1.28E−04 144
    MOR08113 huC5 2.51E+06 6.77E−05 28
    cynoC5 1.53E+06 1.27E−04 83
    MOR08114 huC5 2.09E+06 3.12E−05 15
    cynoC5 1.06E+06 3.13E−05 31
    5G1.1 huC5 1.29E+06 7.22E−05 56
    • 2. Determination of Picomolar Affinities Using Solution Equilibrium Titration (SET) for Purified Fabs or Fabs Bacterial Lysates (Meso Scale Discovery (MSD))
  • For KD determination by solution equilibrium titration (SET), monomer fractions (at least 90% monomer content, analyzed by analytical SEC; Superdex75, Amersham Pharmacia) of Fab protein were used. Affinity determination in solution was basically performed as described in the literature (Friguet et al., J. Immunol Methods 77:305-319 (1985)). In order to improve the sensitivity and accuracy of the SET method, it was transferred from classical ELISA to ECL based technology (Haenel et al., Anal Biochem 339:182-184 (2005).
  • 1 mg/ml goat-anti-human (Fab)2 fragment specific antibodies (Dianova) were labelled with ECL Sulfo-TAG™ NHS-Ester (Meso Scale Discovery, Gaithersburg, Md., USA) according to manufacturers instructions. Experiments were carried out in polypropylene microtiter plates and PBS pH 7.4 with 0.5% BSA and 0.02% Tween 20 as assay buffer. Unlabelled antigen was diluted in 2n series, starting with a concentration at least 10 times higher than the KD. Wells without antigen were used to determine Bmax values; wells with neither antigen nor Fab were used to determine background. After addition of e.g. 10 pM Fab (final concentration in 60 μl final volume), the mixture was incubated over night at RT. The applied Fab concentration was similar to or below the expected KD.
  • Streptavidin MSD plates were coated with 0.2 μg/ml biotinylated human C5 (30 μl/well) and blocked with 5% BSA in PBS. Subsequently the equilibrated samples were transferred to those plates (30 μl per well) and incubated for 20 min. After washing, 30 μl/well of the ECL Sulfo-tag labeled detection antibody (goat anti-human (Fab)2) in a final dilution of 1:1500 was added to the MSD plate and incubated for 30 min on an Eppendorf shaker (700 rpm).
  • After washing and adding 30 μl/well MSD Read Buffer T with surfactant Electrochemiluminescence signals were detected using a Sector Imager 6000 (Meso Scale Discovery, Gaithersburg, Md., USA).
  • Data were evaluated with XLfit (IDBS) software applying customized fitting models. For data evaluation i.e. KD determination of Fab molecules the following fit model was used (model of Abraham et al 16, modified according to et al., 200515): y=Bmax−(Bmax/(2*cFab)*(x+cFab+KD−sqrt((x+cFab+KD)*(x+cFab+KD)−4*x*cFab))); cFab: applied Fab concentration; x: applied total soluble antigen concentration (binding sites); sqrt: square root. Using the assay conditions described above (monomeric) affinities for the affinity-optimized C5-binding Fabs were determined in solution.
    • Parental Fabs
  • In order to further characterize the C5-binding antibodies, affinity of the parental Fabs to human C5 was determined. Because characterization focus was on efficacy in hemolytic assays, affinity measurements were done only for the most relevant Fabs. For a reliable determination of monovalent affinities only Fab batches were used for measurements which showed ≧90% monomeric fraction in a qualitative size exclusion chromatography.
  • Affinities of the 10 parental Fabs which entered affinity maturation are summarized in Table 8. Affinities ranged from 72 pM to 3.7 nM.
  • TABLE 8
    Affinities of the 10 parental Fabs determined in SET
    SET
    MOR0 Number KD [pM]
    6525 72
    6756 1521
    6757 1186
    6763 820
    7086 108
    7087 3793
    7091 324
    7092 229
    7093 576
    7094 1364
    3207 no binding
    (negative control)
    (n = 1)
    • Matured Fabs
  • Monovalent affinities of the purified Fabs to human C5 were measured in SET. Affinities were in the low pM range and best affinities were obtained for derivatives of MOR07086, 7091, 7092 and 7093. Subsequently affinity measurements of these derivatives to cynomolgus C5 showed affinities in the mid to low pM range.
  • The affinity maturation process was very successful resulting in a repertoire of binders with markedly improved affinity. Table 9 summarizes affinities to human and cynomolgus C5 of the best improved binders. Certain Fabs have KD to human C5≦30 pM and to cynomolgus C5≦150 pM.
  • TABLE 9
    Overview of affinities to human and cynomolgus C5 for
    the best affinity improved Fabs
    Set hu C5 (n = 1-2) Set cyno C5 (n = 1) Set hu C5 (n = 1-2) Set cyno C5 (n = 1)
    MOR Matured KD [pM] KD [pM] MOR Matured KD [pM] KD [pM]
    6525 273/29  7871 LCDR3 3 4
    7813 HCRD2 437 7872 LCDR3 2 3
    7814 HCRD2 137 7873 LCDR3 13/13 6
    7816 HCRD2 116 7874 LCDR3 35 8
    6757 3650/1245 7092 96 481
    7818 HCRD2 491  70 7831 HCRD2 10 36
    7907 HCRD2 179 7832 HCRD2 4 13
    6763 673/962 7909 HCRD2 7 18
    7820 HCRD2  62 7910 HCRD2 27 31
    7086 12/65  10 7876 LCDR3 78 60
    7821 HCRD2  7  39 7877 LCDR3 29 144
    7822 HCRD2  5  14 7878 LCDR3 33 70
    7823 HCRD2  5  15 7879 LCDR3 25 122
    7824 HCRD2  55/130 7093 431/992 3146
    7864 LCDR3  22 974 7833 HCRD2 47 107
    7865 LCDR3  10  88 7834 HCRD2 4 15
    7866 LCDR3  10 191 7835 HCRD2 29 28
    7867 LCDR3  19 154 7836 HCRD2 11
    7868 LCDR3 384 7890 HCRD2 46
    7869 LCDR3  2  83 7094
    7870 LCDR3  12 500 7880 LCDR3 13 13
    7087 120 7881 LCDR3 88
    7827 HCRD2 361 7882 LCDR3 70
    7828 HCRD2 2477/1730 7883 LCDR3 49
    7091 135/138 704 7884 LCDR3 83
    7829 HCRD2 429 116 7885 LCDR3 35
    7830 HCRD2 399  75 criterion: KD hu C5 <30 pM; cy C5 <150 pM
    7908 HCRD2   15*  39*
    *scattering (no reliable measurement)
    • 3. KD Determination of IgG Molecules Using Solution Equilibrium Titration (SET)
  • Affinities of the germlined IgGs (human IgG1AA format) to human and cynomolgus C5 were determined in SET as described below. Similar data sets between two independent measurements showed higher affinities of the lead IgGs to human C5 than reference IgG 5G1.1 (see U.S. Pat. No. 6,355,245). Final IgGs had affinities for human C5 ranging from 1 to 14 pM and affinities to cynomolgus C5 ranging from 3 to 29 pM.
  • TABLE 10
    KD values determination for the final lead IgGs
    (human IgG1AA format) in SET
    1st measurement 2nd measurement
    human C5 cyno C5 human C5 cyno C5
    KD [pM] KD [pM] KD [pM] KD [pM]
    hu MOR08109 4 13 2 6
    IgG1AA MOR08110 7 18 3 8
    germlined MOR08111 5 14 3 17
    MOR08113 14 29 8 16
    MOR08114 1 5 2 4
    hu IgG2/4 5G1.1 24 no binding 19 no binding
    (reference
    IgG)
  • For KD determination by solution equilibrium titration (SET), monomer fractions of IgG protein were used (at least 90% monomer content, analyzed by analytical SEC MALS; Tosoh TSKgel G3000SWXL, Wyatt Treos miniDAWN). Affinity determination in solution was basically performed as described in the literature (Friguet et al., J. Immunol Methods 77:305-319 (1985)). In order to improve the sensitivity and accuracy of the SET method, it was transferred from classical ELISA to ECL based technology (Haenel et al., Anal Biochem 339:182-184 (2005)).
  • 1 mg/ml goat-anti-human (Fab)2 fragment specific antibodies (Dianova) were labelled with ECL Sulfo-TAG™ NHS-Ester (Meso Scale Discovery, Gaithersburg, Md., USA) according to the manufacturers instructions. The experiments were carried out in polypropylene microtiter plates and PBS pH 7.4 with 0.5% BSA and 0.02% Tween 20 as assay buffer. Unlabeled antigen was diluted in 2n or 1.75n series, respectively, starting with a concentration at least 10 timer higher than the KD. Wells without antigen were used to determine Bmax values; wells containing neither antigen nor IgG were used to determine background. After addition of e.g. 10 pM IgG (final concentration in 60 μl final volume), the mixture was incubated over night at RT. The applied IgG concentration was similar to or below the expected KD.
  • Streptavidin MSD plates were coated with 0.2 μg/ml biotinylated human C5 (30 μl/well) and blocked with 5% BSA in PBS. Subsequently the equilibrated samples were transferred to those plates (30 μl per well) and incubated for 20 min. After washing, 30 μl/well of the ECL Sulfo-tag labeled detection antibody (goat anti-human (Fab)2) in a final dilution of 1:1500 was added to the MSD plate and incubated for 30 min on an Eppendorf shaker (700 rpm).
  • Electrochemiluminescence signals were detected after washing and adding 30 μl/well MSD Read Buffer T with surfactant using a Sector Imager 6000 (Meso Scale Discovery, Gaithersburg, Md., USA).
  • Data were evaluated with XLfit (IDBS) software applying customized fitting models. For data evaluation i.e. KD determination of IgG molecules the following fit model for IgG was used (modified according to Piehler et al., 199717): y=Bmax/(clgG/2)*(clgG/2−((x+clgG+KD)/2−((x+clgG+KD)̂2/4−x*clgĜ0.5)̂2/(2*IgG)); clgG=applied IgG concentration, complete molecule (not binding sites); x=applied total soluble antigen concentration (binding sites); sqrt: square root.
    • Example 8 Characterization by Hemolytic Assays
  • The hemolytic assay is a basic functional assay that tests for complement activation and has been used to evaluate the ability of anti-human C5 mAbs and Fab molecules to block lysis of red blood cells (RBCS) by complement pathways (see e.g., Evans et al., Mol. Immunol 32: 1183-1195 (1995); Thomas et al., Mol Immunol 33:1389-1401 (1996); Rinder et al., J Clin Invest 96:1564-1572 (1995)). Briefly, for classical pathway assays, sensitized red blood cells are used as targets for lysis by complement proteins present in serum. This assay is of interest for the characterization and screening of high-affinity anti-human C5 mAbs.
    • 1. Classical Pathway
  • The desired number of chicken red blood cells was washed four times with cold gelatin veronal buffer (GVB++) and resuspended to 5×107 cells/ml. To sensitize the cells rabbit anti-chRBC IgG was added to RBC cell suspension to a final concentration of 1 μg/ml IgG. After 15 minutes incubation on ice, the sensitized chRBCs were centrifuged, washed twice with GVB++ and diluted to 8.33×107 cells/ml.
  • Round-bottom 96 well plates were used for hemolytic assay. Antibodies were diluted in GVB++ buffer and added to the wells (when calculating the required concentration of C5-binding Abs, it was considered that the sample will be diluted two-fold when serum is added). 50 μl of 40% human serum (diluted in GVB++) was added to 50 μl antibody dilutions, resulting in a final serum assay concentration of 20%.
  • The control and blank wells were prepared as described here: control wells: i) 0% lysis control→100 μl GVB++, ii) 100% lysis control→100 μl 0.1% NP-40, iii) 20% serum control→100 μl of 20% serum (0% Ab control). blank wells: i) 20% serum blank→100 μl 20% serum, ii) GVB++ blank→100 μl GVB++, iii) NP-40 blank→100 μl 0.1% NP-40.
  • 2.5×106 (30 μl) sensitized chRBCs/well were added to all sample and control wells. To the blank wells PBS was added instead of cells. Assay plate was incubated 30 min at 37° C., centrifuged (2.000 rpm, 5 min) and 85 μl supernatant was transferred to a new, flat-bottomed 96-well plate. The new plate was centrifuged (2.000 rpm, 3 min) to get rid of any bubbles. Hemoglobin release was measured by reading absorbance at 415 nm. Percentage of hemolysis was calculated with respect to the control and blank wells using the following calculation algorithms:
  • % Hemolysis = 100 × ODsample - ODnegativecontrol ODpositivecontrol - ODnegativecontrol where ODsample = ⌊ AverageOD sample ⌋ - [ AverageOD 20 % SerumBlank ] ODnegativecontrol = ⌊ AverageOD 0 % Lysis ⌋ - [ AverageOD GVB ++ Blank ] ODpositivecontrol = ⌊ AverageOD 100 % Lysis ⌋ - [ AverageOD NP - 40 Blank ]
  • Using this procedure, anti human-05 antibodies which were able to inhibit red blood cell lysis could be identified. To screen for cross-reactivity to cynomolgus C5, the classical pathway was performed using 5% cynomolgus serum.
    • 2. Alternative Pathway
  • Hemolytic assays undergoing the alternative pathway were done in a similar way to the classical pathway hemolytic assays. In the alternative pathway RBCs cells from rabbit were used and there was no need to sensitize the cells. The rabbit RBCs are different from chicken RBCs in that they are sensitive to lysis caused by the complement alternative pathway.
  • The working buffer was GVB++ supplemented with 10 mM EGTA and 5 mM Mg++, since the C5 convertase of the alternative pathway is Mg++ dependent and the C5 convertase of the classical pathway is Ca++ dependent.
  • Hemolytic assays of the alternative pathway were run with: i) 20% human serum, ii) 100 pM human C5 added to 20% human C5-depleted serum, iii) 0.025% cynomolgus serum added to 20% human C5-depleted serum, iv) 100 pM cynomolgus C5 added to 20% human C5-depleted serum, v) 10% cynomolgus serum. These settings were used to screen for antibodies with high affinity to the human and cynomolgus C5 proteins which were able to inhibit very effectively the red blood cell lysis induced by the alternative complement pathway.
  • 3. Hemolytic Assays with Parental Fabs
  • Hemolytic assays were used as a basic bio-functional assay to evaluate the ability of anti-human C5 mAbs to block complement mediated lysis of red blood cells. C5 convertase cleaves C5 into C5a peptide and C5b fragment, that is subsequently incorporated into the membrane-attack complex (MAC), which leads to cell lysis. C5 convertase of the classical pathway, formed by a C3bC4bC2a complex has a different structure than the C5 convertase of the alternative pathway which is formed by a C3bC3bBb complex. HuCAL GOLD® generated antibodies should be inhibitory in both classical and alternative pathway, but with focus on the alternative pathway because mainly the alternative pathway (factor H, factor B and factor H-related genes) is implicated in AMD.
  • The classical and alternative pathway assays were performed with 20% human serum (˜80 nM C5). To increase sensitivity of alternative pathway assays, new assay formats were developed. 10-100 pM purified human C5 or 0.025% cynomolgus serum (˜100 pM cynomolgus C5) were added to human C5-depleted serum (but containing all other serum and complement components).
  • FIG. 2 shows that considerable hemolysis could be observed between 10 and 100 pM purified human C5 added to human C5-depleted serum. Cynomolgus serum was added to human C5-depleted serum to test for cross-reactivity. FIG. 3 shows that 0.025% of cynomolgus serum (˜100 pM C5) added to human C5 depleted serum restores hemolytic activity.
    • Classical Pathway
  • First Fab selection was done in the classical pathway (20% human serum). Approximately half of the 61 purified parental Fabs were weak to strong inhibitors of the classical pathway. IC50 values of the best inhibitory Fabs were between 35 and 900 nM.
  • Assays were done showing congruent results (as shown in FIG. 4). % hemolysis was calculated with respect to the control and blank wells. Fab inhibition of cell lysis was compared to a maximum lysis caused by 20% human serum (=100%). An irrelevant human Fab (hen egg white lysozyme binder MOR03207) was used as negative control and anti-human C5 IgG monoclonal antibody (Quidel) as positive control. FIG. 4 show an example with the best inhibitory Fabs.
    • Alternative Pathway
  • Fabs which showed inhibitory activity in the classical pathway were further evaluated in the alternative pathway. Hemolytic assays were run with 100 pM purified human C5 or 0.025% cynomolgus serum added to human C5-depleted serum. IC50 values for the human alternative assays were between 0.1 and 90 nM (examples of assays with the most relevant Fabs are shown in FIG. 5.
  • The positive control of the classical pathway (anti-human C5 antibody, Quidel) was not inhibitory in the alternative pathway. Therefore an anti-complement factor P antibody (Quidel) was used as positive control. As shown in FIG. 5, MOR07086 had best inhibitory activity and NVS data revealed a better potency than for the reference antibody 5G1.1.
  • To test for cynomolgus cross-reactivity, hemolytic assays of the alternative pathway were performed with 0.025% cynomolgus serum added to human C5-depleted serum. A comparison to 5G1.1 was not possible, since 5G1.1 does not recognize cynomolgus C5. The anti-Factor P antibody was used as positive control. Results of assays revealed IC50 values between 0.1 and 400 nM for the best inhibitory Fabs. Again, MOR07086 showed best potency (shown in FIG. 6).
  • A consistent inhibitory activity of the Fabs was noticed in both classical and alternative pathway. Table 11 below summarizes the results of hemolytic assays for the most relevant 22 Fabs. To have a reliable comparison between different experiments, lysis caused by 20% human serum was normalized to 100%.
  • TABLE 11
    Summary of hemolytic assays with the most relevant Fabs
    MOR IC50 [nM] NVS IC50 [nM]
    AP (0.1 nM C5) AP (0.025% cyno AP (0.1 nM C5) AP (0.025% cyno
    CP [human] [human] serum) [cyno] CP [human] [human] serum) [cyno]
    MOR-Nr normalized normalized normalized normalized normalized normalized
    6525    190  15     11 185   7 5
    6756    320  80     400 225   70 2500
    6757    500  90     30 305  130 25
    6763    250  45     110 195   20 360
    6764 n.t.  50 n.t. n.t   25 30% inh
    6776 >4000  40 n.t.   20* 50% inh
    6952    90  20  >1000 110   15 200
    6961    100  25     600 85   15 30
    7081    180   5 40% inh 170   3 10
    7082    70 2.5      1 90   1 1
    7083    100  30     300 140   10 5
    7084    120  10    1.2 160   5 1.5
    7086    35 0.2/0.2 0.2/0.4 85  0.1 0.1
    7087 >4000  50     100 775   10 1
    7088    110  15     230 130   5 15
    7089    150  75     900 250   20 50
    7090    105  20     10 120   10 1
    7091    82   7     40 110   3 4
    7092    100   1    1.5 90  0.5 1.5
    7093 >4000   7     190 230   5 15
    7094 770   40 190
    7095*   120*  0.5**     1.3** n.t.
    *not pure as MH
    **as pMx9_FS

    4. Hemolytic Assays with Matured Fabs
    • Classical Pathway (1) Classical Pathway Using 20% Human Serum
  • Matured Fabs were tested in the classical pathway with 20% human serum. Derivatives of MOR07086, 7091, 7092 and 7093 showed highest potency (IC50 values in the low nM range). Descendants of MOR07091, 7092 and 7093 showed strongly improved potency. FIG. 7 shows examples of hemolytic assays with derivatives of MOR07086, 7091, 7092 and 7093.
    • (2) Classical Pathway Using 5% Cynomolgus Serum
  • Assays of the complement pathway were also run in the presence of 5% cynomolgus serum in order to test for cross-reactivity. Derivatives of MOR07086, 7091, 7092 and 7093 could very effectively inhibit red blood cell lysis. The negative control, MOR03207 (anti-lysozyme Fab), had no impact on the complement pathway. Results of these assays are shown in FIG. 8.
    • Alternative Pathway (1) Alternative Pathway Using 100 pM Human C5
  • Matured Fabs were tested in the alternative pathway hemolytic assay with 100 pM human C5. Some derivatives of MOR06525, 6757, 6763, and 7087 showed potency improvement compared to their parentals. MOR07086-, 7091-, 7092-, 7093-, and 7094-derived Fabs showed highest potency (IC50 values in the low nM range). Descendants of MOR07091, 7092, 7093, and 7094 showed highly improved potency, many of which are more potent than reference antibody 5G1.1. FIG. 9 shows examples of hemolytic assay results for the affinity matured Fabs and 5G1.1.
    • (2) Alternative Pathway Using 20% Human Serum
  • Matured Fabs were tested in the alternative pathway hemolytic assay with 20% human serum. MOR07086-, 7091-, 7092- and 7093-derived Fabs showed best inhibitory activity. Many of these Fabs had better inhibitory activity than 5G1.1. FIG. 10 shows examples of hemolytic assay results for the affinity matured Fabs and reference antibody 5G1.1.
    • (3) Alternative Pathway Using 100 pM Cynomolgus C5
  • Matured Fabs were tested in the alternative pathway hemolytic assay using 100 pM cynomolgus C5 added to 20% human C5-depleted serum. MOR07091-, 7092- and 7093-derived Fabs showed best inhibitory activity; 5G1.1 does not crossreact with cynomolgus C5. FIG. 11 shows examples of hemolytic assay results for the affinity matured Fabs.
  • 5. Hemolytic Assays with Germlined IgGs (Human IgG1AA Format)
    • Classical Pathway (1) Classical Pathway Using 20% Human Serum
  • Classical pathway assays using 20% human serum were run at MOR. IC50 values of the final germlined hu IgGAA—MOR08109, 8110, 8113, 8114—were better or similar to reference IgG 5G1.1 (see FIG. 12).
    • (2) Classical Pathway Using 5% Cynomolgus Serum
  • A comparison to 5G1.1 in the classical pathway using 5% cynomolgus serum was not applicable, since this reference antibody does not recognize cynomolgus C5. The final germlined IgGs could completely inhibit lysis of the red blood cells induced by cynomolgus serum except MOR08111. Data are shown in FIG. 13.
    • Alternative Pathway (1) Alternative Pathway Using 100 pM Human C5
  • The germlined IgGs were tested in the alternative pathway hemolytic assay using 100 pM human C5. All antibodies showed potent inhibitory activity with IC50 values between 28 and 128 pM (with the exception of MOR08111, see FIG. 14), all were equal to or better than 5G1.1. FIG. 14 shows examples of hemolytic assay results for the IgGs.
    • (2) Alternative Pathway Using 20% Human Serum and C5a Generation ELISA
  • The germlined IgGs were also tested in the alternative pathway hemolytic assay with 20% human serum. The majority of the antibodies tested achieve complete inhibition with IC50 values lower than 80 nM. Reference antibody 5G1.1 does not fully inhibit hemolysis in this assay. FIG. 15 shows examples of hemolytic assay results for the IgGs. Inhibition of C5a generation by the final IgGs was similar to 5G1.1 (IC50 values in the low nM range).
    • (3) Alternative Pathway Using 100 pM Cynomolgus C5
  • Hemolytic assays of the alternative pathway in 20% human C5-depleted serum were reconstituted with 100 pM cynomolgus C5. Potency of the germlined final candidates against cynomolgus C5 was within 5-fold of that for human C5 (IC50 values in the low pM range).
    • (4) Alternative Pathway Using 10% Cynomolgus Serum
  • In hemolytic assays of the alternative pathway using 10% cynomolgus serum ([C5]˜40 nM) the potency of the germlined candidates was similar to the potency in human serum (success criterion was to have a potency not more than 5-fold weaker than for the functional assay using human C5).
    • Example 9 C5a Generation ELISA
  • C5a-des-Arg ELISA was developed to measure C5a generation during hemolysis to confirm that antibodies that were inhibitory in the hemolytic assay also inhibited cleavage of C5 into C5a and C5b.
  • A Maxisorp plate was coated with 100 μl/well mouse anti-human C5a-des-Arg (US Biologics) at 1 μg/ml in coating buffer (bicarbonate pH 9.5-9.8) and was incubated overnight at 4° C. After washing 3× with PBST, the plate was blocked with 300 μl/well diluent (Synblock, AbD Serotec) for 2 hours at room temperature. After aspirating the blocking solution, 100 μl samples or standards diluted with diluent were incubated for 1 hour at room temperature. Standards were prepared as follows: start was at 20 ng/ml standard (rC5a-des-Arg) and 1:4 serial dilutions were prepared for a 7-point curve. Samples of hemolytic assays were diluted 1:5 in diluent (hemolytic assay supernatants should be stored at −80° C. until used in C5a ELISA). In between the plate was washed 3× with PBST. 100 μl/well of 0.4 μg/ml detection antibody (biotin-goat anti-human c5a, R&D Systems) diluted in diluent was added and after 1 hour incubation at room temperature, 100 μl/well Strep-HRP (poly-HRP streptavidin) diluted 1:5000 in HRP diluent (poly-HRP diluent) was added for 30 minutes. After washing 4× with PBST, 100 μl/well TMB Substrate (Ultra TMB substrate solution) was added for 5-10 minutes. Reaction was stopped with 50 μl/well stop solution (2N H2SO4). Absorbance was read (A450-A570) and data were analyzed using SoftMax Pro.
  • Matured Fabs were tested for C5a generation during hemolysis to confirm that inhibitory activity was due to blocking C5 cleavage into C5a and C5b. The supernatants from hemolytic assays in 20% human serum were used for quantifying the C5a formation.
  • All Fabs tested brought C5a levels down to baseline. FIG. 16 shows examples of C5a ELISA results.
    • Example 10 Specificity ELISA on Human C3, C4, C5 and Cynomolgus C5
  • All purified Fabs were analyzed in a solution ELISA (method described above) for binding to human C3, C4 and C5. Fabs were incubated with biotinylated antigen on a Neutravidin plate and detected via the histidin tag.
  • Improved binding was seen for almost all matured Fabs compared to their respective parental. No binding to the counter targets human C4 and C3 was detected up to 100 nM Fab. These results hit the success criteria for specificity: binding to human and cynomolgus C5 and no binding to human complement proteins C3 and C4. Examples for derivatives of parental Fab MOR07091 are shown in FIG. 17.
    • Example 11 Serum Stability Assays
  • Retained binding activity to human C5 in a binding assay at 50% human serum of C5-binding antibodies was determined as described below.
  • Antibodies (Fab format) were incubated up to 8 h at 37° C. with 100% human C5-depleted serum or with PBST/0.5% BSA (positive control). Wells of a blocked polypropylene plate were used for incubation to ensure no binding of the antibodies to the surface over the long incubation time. Samples were collected at different time points and stored at −20° C.
  • Samples were tested in a solution ELISA on NeutrAvidin plates to check binding ability to human C5. To the NeutrAvidin plates, which were blocked o/n with 1× ChemiBlocker-PBST. 20 μl of serial dilutions of the different collected samples were added. First dilution of the samples was 1:2 (final serum concentration 50%), followed by 1:3 dilutions steps. After 1 h incubation the plate was washed 3× with PBST and 20 μl biotinylated human C5 was applied to a concentration of 2.5 μg/ml. After 1 h plate was washed again 5× with PBST (0.05% Tween) and anti-HIS6-POD detection antibody for Fabs was added.
  • Fluorescence of the substrate (Quanta Blue or AttoPhos) was measured after 5-10 min and retained binding activity was calculated compared with the respective maximum signal (antibody incubated with PBST/0.5% BSA).
  • One of the “must” criteria for the C5-binding antibodies is to retain 75-80% of binding activity in human serum i) in a functional assay at 10% serum and ii) in a binding assay at 50% serum. Because hemolytic assays were run in the presence of 20% serum it was only necessary to show retained binding in a binding assay at 50% serum.
  • Therefore matured final Fabs were incubated with 100% human C5-depleted serum at 37° C. for 8 h. Samples were collected at different time points and tested for binding to human C5 in a solution ELISA. Fab+serum samples used for ELISA were diluted to a concentration of 50% serum+10 nM Fab.
  • FIG. 18 illustrates the results of the final C5-binding final antibodies in the Fab format. 70-93% of the binding activity was retained after an 8 hour incubation time at 37° C. in 50% serum compared to incubation in PBS.
    • Example 12 Characterization by Epitope Binning
  • This procedure was used to group anti-human C5 Fabs into different epitope bins binding to the same or an overlapping epitope of the C5 protein.
  • Competition of each biotinylated anti-human C5 antibody with each unlabelled anti-human C5 antibody in 100-fold excess was tested in an ELISA (capture mode). It was compared with the highest signal of each antibody (biotinylated Fab without competition).
  • Human C5 was captured via a polyclonal anti-human C5 IgG (US Biological), which was coated previously o/n at 4° C. on a 384 well black Maxisorp plates. Next day the plate was washed twice with PBST and blocked for 2 h with 3% BSA-PBST. After washing 3× with PBST, 20 μl human C5 was added and incubated 2 h ar RT. The plate was washed 3× with PBST before adding the Fabs.
  • 20 μl unlabelled Fab (200 μg/ml or 400 μg/ml) (100-fold excess) was added to the wells of a Maxisorp plate and subsequently 20 ng/ml or 40 ng/ml of biotinylated Fab. The biotinylated and unlabelled Fabs were incubated for 1 h at RT. The plate was washed 3× with PBST and Strep-AP Zymax Streptavidin-Alkaline Phosphatase, ZYMED, Code: 43-8322, Lot: 50799648 was added for detection of the biotinylated Fab binding via C5 to the plates. AttoPhos substrate (Roche) was added to the plates and Fluorescence was read after 5-10 min.
    • Parental Fabs
  • C5 was captured (via a polyclonal antibody) and unlabelled FabY was applied in excess to biotinylated FabX. Binding of biotinylated FabX to human C5 was detected. Six groups of Fabs could be defined: Group 1: MOR06952, 6961; Group 2: MOR06525, 6756, 6757, 6763; Group 3: MOR07087; Group 4: MOR06764, 6776, 7081; Group 5: MOR07089; Group 6: MOR07082, 7083, 7084, 7086, 7088, 7090, 7091, 7092, 7093, 7095.
  • The Fabs were also divided into different epitope binding groups using a different method: FabX was immobilized, then FabY pre-incubated with biotinylated C5 was added. Following groups of Fabs could be defined: Group 1: MOR06952, 6961; Group 2: MOR06525,6757, 7083; Group 3: MOR07087; Group 4: MOR06763; Group 5: MOR07081; Group 6: MOR07082, 7083, 7084, 7086, 7088, 7091, 7092, 7093 (7089 competes with 7084). The conclusion was drawn that using two different methods, similar results could be obtained.
    • Matured Fabs
  • In order to complete Fab characterization competition of biotinylated Fab with unlabelled Fab (applied in 100-fold excess) was measured in solution ELISA. Results were compared with the highest signal (biotinylated Fab without competition).
  • As shown in FIG. 19, biotinylated Fabs compete with identical unlabelled Fabs and all Fabs compete for binding to the same or overlapping epitope. These results correlate with epitope binning data for the parental Fabs.
    • Example 13 Screening of C5 Alpha Versus Beta Chain Binders and Competition Assays
  • Two ELISA experiments and hemolytic assays were performed to test if a Fab was an alpha or beta chain binder as described below.
  • In the first experiment, Fab was coated on a plate and purified C5 or supernatant from chimeric C5 preparation (human alpha, mouse beta chain) was added. As a next step 5G1.1 was applied and detection was done via an anti-human IgG.
  • In a second experiment, 5G1.1 was coated on a plate, purified C5 or supernatant from chimeric C5 preparation (human alpha, mouse beta chain) was added, then Fab, which was detected with an anti-Myc antibody.
  • Reference IgG 5G1.1 recognizes the alpha chain and was used to determine if the MorhpSys generated Fabs compete with 5G1.1 for binding. In the hemolytic assays supernatant from chimeric C5 preparation was added to human C5-depleted serum and Fabs were tested for inhibition of hemolysis.
    • Parental Fabs
  • FIG. 20 shows the results of an ELISA experiment where the Fabs were coated on a plate, C5 or supernatant of a chimeric C5 preparation (human alpha chain and mouse beta chain) was added, then 5G1.1. FIG. 21 shows the results of an ELISA experiment where purified C5 and supernatant from chimeric C5 were captured via 5G1.1.
  • MOR06525, 6756, 6763 were beta chain binders (bind to C5 but not chimeric C5). Most MOR070XX Fabs (derived from solution pannings) are alpha chain binders (bind to C5 and chimeric C5). MOR06952 and 6961 compete with 5G1.1 so they are negative for both C5 and chimeric C5 and, thus, are most likely alpha chain binders as 5G1.1. MOR06757 behaves like MOR06952 and 6961, i.e. it likely is an alpha chain binder. However, MOR06757 does not inhibit hemolysis of chimeric C5 supernatant spiked into C5-depleted serum, while all the other alpha chain binders do (see FIG. 22).
  • In the hemolytic assay supernatant from chimeric C5 prep was added to human C5-depleted serum and Fabs were tested for inhibition of hemolysis. MOR06525, 6756, 6757 and 6763 did not inhibit hemolysis with chimeric C5 and thus, could be beta chain binders. MOR06952, 6961, 7081, 7082, 7083, 7084, 7086, 7087, 7088, 7089, 7090, 7091, 7092, 7093, 7094, 7095 inhibited hemolysis and thus could be alpha chain binders.
    • Example 14 Resistance to Proteolysis
  • To investigate the structural rigidity of Fabs, resistance of Fabs to proteolysis by thermolysin was performed (thermolysin bacterial protease, Calbiochem). Fab was incubated with thermolysin (Fab:thermolysin=3:1 (w/w), reaction volume of 8 μL) either at 37° C. or at 55° C. (thermolysin activity is optimal at 55° C.). The reaction was stopped by adding 4 μL of 0.5 M EDTA and 4 μL of 4×LDS sample buffer (Invitrogen) and the stopped samples were run on 4-12% SDS-PAGE at non-reducing condition. Proteolysis of Fabs was analyzed by monitoring the disappearance of Fab bands that were visualized by Coomassie staining.
    • Parental Fabs
  • Parental Fabs were tested for resistance to thermolysin proteolysis at 37° C. and 55° C. Fab from a humanized IL-1β antibody was used as control. Most tested Fabs were resistant to degradation by thermolysis at 37° C. up to 90 min. To further differentiate the structural rigidity of Fabs, proteolysis was performed at higher temperature of 55° C. Many of the Fabs tested were quickly degraded at 55° C. (>90% Fab was degraded within 30 min), while some Fabs were still resistant to proteolysis after 90 min (e.g., 7094). The resistant Fabs were suggested to have a more rigid structure such that they might show better in vivo pharmacokinetic properties. Results of these experiments are shown in the FIG. 23 and FIG. 24.
    • Matured Fabs
  • Fabs with the highest potency in hemolytic assays were tested for sensitivity to thermolysin at 37° C. and 55° C. In FIG. 25 and FIG. 26, experiments with derivatives of MOR07086, 7091, 7092 and 7093 are shown.
  • Results of these tests revealed that derivatives of parentals MOR07091, 7092 and 7093 were less sensitive to proteolysis, while MOR07086 derivatives were more sensitive to proteolysis.
    • Example 15 MAC Deposition Assay
  • As the terminal complement cascade ends up with formation of the MAC, inhibition of MAC formation was a further hint for the antibody ability to block the complement cascade. The rational was to have an additional set-up independent of cells and cell behaviour
  • Zymosan (Sigma), which is an insoluble carbohydrate from the cell wall of yeast, used especially in the immunoassay of the alternative pathway, was coated to activate the Alternative Pathway and IgM (Sigma) was coated to activate the Classical Pathway for determination of MAC (membrane attack complex) deposition. Fabs were pre-incubated with human serum (6% for AP, 2% for CP) and added to plate. Percentage (%) inhibition of MAC deposition was calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum), and used to generate the IC50 curve with XLFit.
    • Parental Fabs
  • Parental Fabs were used in different concentrations and the maximal inhibition (if applicable also IC50 values) were determined (example shown in FIG. 27). Most Fabs completely inhibited MAC deposition indicating blocking of C5 cleavage. Potency and ranking of Fabs were similar to data from hemolytic assays.

Claims (12)

1-16. (canceled)
17. An isolated antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region having the sequence of SEQ ID NO: 7 and a light chain variable region having the sequence of SEQ ID NO: 8.
18. The antibody, or an antigen binding fragment thereof, of claim 17, wherein said antibody is a monoclonal antibody.
19. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody is a human or humanized antibody.
20. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody is a chimeric antibody.
21. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody comprises a human heavy chain constant region and a human light chain constant region.
22. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody is a single chain antibody.
23. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody is a Fab fragment.
24. The antibody, or an antigen binding fragment thereof, of claim 18, wherein said antibody is a scFv.
25. The antibody, or an antigen binding fragment thereof, of claim 18 binds to both human complement C5 and cynomolgus complement C5.
26. The antibody of claim 18, or an antigen binding fragment thereof, is an IgG isotype.
27. The antibody, or an antigen binding fragment thereof, of claim 17, wherein said antibody comprises a heavy chain having the sequence of SEQ ID NO: 9 and a light chain having the sequence of SEQ ID NO: 10.
US14/698,039 2008-08-05 2015-04-28 Compositions and Methods for Antibodies Targeting Complement Protein C5 Abandoned US20160031975A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/698,039 US20160031975A1 (en) 2008-08-05 2015-04-28 Compositions and Methods for Antibodies Targeting Complement Protein C5
US15/354,978 US10407496B2 (en) 2008-08-05 2016-11-17 Compositions and methods for antibodies targeting complement protein C5

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8635508P 2008-08-05 2008-08-05
US12/535,205 US8241628B2 (en) 2008-08-05 2009-08-04 Compositions and methods for antibodies targeting complement protein C5
US13/535,612 US20130022615A1 (en) 2008-08-05 2012-06-28 Compositions and Methods for Antibodies Targeting Complement Protein C5
US14/698,039 US20160031975A1 (en) 2008-08-05 2015-04-28 Compositions and Methods for Antibodies Targeting Complement Protein C5

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US13/535,612 Continuation US20130022615A1 (en) 2008-08-05 2012-06-28 Compositions and Methods for Antibodies Targeting Complement Protein C5

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US201615206916A Continuation 2008-08-05 2016-07-11

Publications (1)

Publication Number Publication Date
US20160031975A1 true US20160031975A1 (en) 2016-02-04

Family

ID=41227252

Family Applications (5)

Application Number Title Priority Date Filing Date
US12/535,205 Active 2030-02-16 US8241628B2 (en) 2008-08-05 2009-08-04 Compositions and methods for antibodies targeting complement protein C5
US13/535,612 Abandoned US20130022615A1 (en) 2008-08-05 2012-06-28 Compositions and Methods for Antibodies Targeting Complement Protein C5
US13/557,562 Active US8883158B2 (en) 2008-08-05 2012-07-25 Compositions and methods for antibodies targeting complement protein C5
US14/698,039 Abandoned US20160031975A1 (en) 2008-08-05 2015-04-28 Compositions and Methods for Antibodies Targeting Complement Protein C5
US15/354,978 Active 2029-09-22 US10407496B2 (en) 2008-08-05 2016-11-17 Compositions and methods for antibodies targeting complement protein C5

Family Applications Before (3)

Application Number Title Priority Date Filing Date
US12/535,205 Active 2030-02-16 US8241628B2 (en) 2008-08-05 2009-08-04 Compositions and methods for antibodies targeting complement protein C5
US13/535,612 Abandoned US20130022615A1 (en) 2008-08-05 2012-06-28 Compositions and Methods for Antibodies Targeting Complement Protein C5
US13/557,562 Active US8883158B2 (en) 2008-08-05 2012-07-25 Compositions and methods for antibodies targeting complement protein C5

Family Applications After (1)

Application Number Title Priority Date Filing Date
US15/354,978 Active 2029-09-22 US10407496B2 (en) 2008-08-05 2016-11-17 Compositions and methods for antibodies targeting complement protein C5

Country Status (37)

Country Link
US (5) US8241628B2 (en)
EP (3) EP2837388A1 (en)
JP (2) JP5815403B2 (en)
KR (1) KR101463296B1 (en)
CN (2) CN102170906B (en)
AR (1) AR072897A1 (en)
AU (1) AU2009279197B2 (en)
CA (1) CA2732782C (en)
CL (1) CL2011000233A1 (en)
CO (1) CO6351750A2 (en)
CR (2) CR20110035A (en)
CU (1) CU23978B1 (en)
DK (1) DK2328616T3 (en)
EA (1) EA027575B1 (en)
EC (1) ECSP11010805A (en)
ES (2) ES2541142T3 (en)
HK (4) HK1202269A1 (en)
HN (1) HN2011000357A (en)
HR (1) HRP20150799T1 (en)
HU (1) HUE026179T2 (en)
IL (1) IL210768B (en)
JO (1) JO3118B1 (en)
MA (1) MA32535B1 (en)
MX (2) MX2011001371A (en)
MY (1) MY159396A (en)
NZ (2) NZ590634A (en)
PE (1) PE20110225A1 (en)
PL (1) PL2328616T3 (en)
PT (1) PT2328616E (en)
RS (1) RS54113B1 (en)
SG (1) SG10201405377XA (en)
SI (1) SI2328616T1 (en)
SV (1) SV2011003834A (en)
TW (2) TW201350130A (en)
UA (1) UA105009C2 (en)
WO (1) WO2010015608A1 (en)
ZA (1) ZA201100375B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017218515A1 (en) 2016-06-14 2017-12-21 Regeneron Pharmaceuticals, Inc. Anti-c5 antibodies and uses thereof
WO2019118556A1 (en) 2017-12-13 2019-06-20 Regeneron Pharmaceuticals, Inc. Anti-c5 antibody combinations and uses thereof
US10407496B2 (en) * 2008-08-05 2019-09-10 Novartis Ag Compositions and methods for antibodies targeting complement protein C5

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2892925T3 (en) 2006-03-31 2022-02-07 Chugai Pharmaceutical Co Ltd Methods for monitoring the blood pharmacokinetics of antibodies
AU2007261315B2 (en) 2006-06-21 2012-10-25 Musc Foundation For Research Development Targeting complement factor H for treatment of diseases
JP5781762B2 (en) * 2007-08-10 2015-09-24 プロテリックス、インク Universal type III fibronectin binding domain library
US8470966B2 (en) 2007-08-10 2013-06-25 Protelica, Inc. Universal fibronectin type III binding-domain libraries
US8680019B2 (en) * 2007-08-10 2014-03-25 Protelica, Inc. Universal fibronectin Type III binding-domain libraries
CN106519025B (en) 2007-09-26 2021-04-23 中外制药株式会社 Method for changing isoelectric point of antibody by using amino acid substitution of CDR
NZ588507A (en) 2008-04-11 2012-11-30 Chugai Pharmaceutical Co Ltd Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly
NZ592786A (en) 2008-11-10 2013-02-22 Alexion Pharma Inc Methods and compositions for treating complement-associated disorders using eculizumab
AR076284A1 (en) 2009-04-29 2011-06-01 Bayer Schering Pharma Ag IMMUNOCONJUGADOS OF ANTIMESOTELINA AND USES OF THE SAME
ME01699B (en) 2009-07-02 2014-09-20 Musc Found For Res Dev Methods of stimulating liver regeneration
EP2496259B1 (en) 2009-11-05 2017-02-22 Alexion Pharmaceuticals, Inc. Treatment of paroxysmal nocturnal hemoglobinuria
JP5986990B2 (en) 2010-05-14 2016-09-06 ザ リージェンツ オブ ザ ユニヴァーシティ オブ コロラド，ア ボディ コーポレート Improved complement receptor 2 (CR2) targeting group
KR20130036276A (en) 2010-06-22 2013-04-11 더 리젠츠 오브 더 유니버시티 오브 콜로라도, 어 바디 코포레이트 Antibodies to the c3d fragment of complement component 3
JP5964300B2 (en) * 2010-08-02 2016-08-03 マクロジェニクス，インコーポレーテッド Covalently bonded diabody and its use
SG10201509790YA (en) 2010-11-30 2015-12-30 Chugai Pharmaceutical Co Ltd Antigen-Binding Molecule Capable Of Binding To Plurality Of Antigen Molecules Repeatedly
WO2012088247A2 (en) * 2010-12-22 2012-06-28 Medimmune, Llc Anti-c5/c5a/c5adesr antibodies and fragments
KR20230005405A (en) 2011-02-25 2023-01-09 추가이 세이야쿠 가부시키가이샤 FcγRIIb-specific Fc antibody
EP2731970B1 (en) * 2011-07-15 2018-11-28 MorphoSys AG Antibodies that are cross-reactive for macrophage migration inhibitory factor (mif) and d-dopachrome tautomerase (d-dt)
WO2013047748A1 (en) 2011-09-30 2013-04-04 中外製薬株式会社 Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities
BR112014013081A2 (en) 2011-11-30 2020-10-20 Chugai Seiyaku Kabushiki Kaisha drug-containing cell vehicle for formation of an immune complex
CA2859493A1 (en) * 2011-12-21 2013-06-27 Novartis Ag Compositions and methods for antibodies targeting factor p
US9808502B2 (en) * 2012-02-20 2017-11-07 Swedish Orphan Biovitrum Ab (Publ) Polypeptides binding to human complement C5
CN104220047B (en) 2012-05-25 2019-11-15 诺华股份有限公司 Aqueous pharmaceutical compositions containing biopharmaceuticals and guanidine or guanidine derivatives and the injection comprising the composition
US10413620B2 (en) 2012-08-17 2019-09-17 The Regents Of The University Of Colorado, A Body Corporate Light-emitting versions of the monoclonal antibody to C3D (MAB 3D29) for imaging
WO2014028865A1 (en) 2012-08-17 2014-02-20 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for detecting complement activation
RU2729831C2 (en) 2012-08-24 2020-08-12 Чугаи Сейяку Кабусики Кайся Versions of fcγriib-specific fc-region
EP2912065A4 (en) * 2012-10-25 2016-10-19 True North Therapeutics Inc Anti-complement c1s antibodies and uses thereof
RU2670943C9 (en) 2012-11-08 2018-11-26 Илэвэн Байотерапьютикс, Инк. Il-6 antagonists and uses thereof
AP2015008365A0 (en) * 2012-12-05 2015-04-30 Novartis Ag Compositions and methods for antibodies targeting epo
BR112015018438A2 (en) * 2013-01-31 2017-07-18 Seoul Nat Univ R&Db Foundation c5 antibody and method for preventing and treating complement-related diseases
JP6505079B2 (en) 2013-03-29 2019-04-24 アレクシオン ファーマシューティカルズ， インコーポレイテッド Compositions and methods for increasing the serum half-life of a therapeutic that targets complement C5
SG11201508170TA (en) 2013-04-02 2015-11-27 Chugai Pharmaceutical Co Ltd Fc REGION VARIANT
CN105683759B (en) 2013-08-07 2019-03-29 瑞颂医药公司 Atypia hemolytic uremic syndrome (aHUS) biomarker protein
CN105658228B (en) 2013-08-28 2020-02-07 瑞典孤儿比奥维特鲁姆有限公司 Stable polypeptides that bind human complement C5
JP7138411B2 (en) 2013-08-28 2022-09-16 アフィボディ・アーベー Binding polypeptides with mutated backbones
US20170073754A1 (en) 2014-02-07 2017-03-16 Novartis Ag Impact of genetic factors on disease progression and response to anti-c5 antibody in geographic atrophy
DK3107935T3 (en) * 2014-02-26 2020-09-21 Allergan Inc COMPLEMENT COMPONENT C5 ANTIBODIES
PL3154561T3 (en) * 2014-06-12 2019-12-31 Ra Pharmaceuticals, Inc. Modulation of complement activity
EP3160991A2 (en) 2014-06-25 2017-05-03 Novartis AG Compositions and methods for long acting proteins
US20170290876A1 (en) 2014-06-25 2017-10-12 Novartis Ag Compositions and methods for long acting proteins
WO2016020791A1 (en) 2014-08-05 2016-02-11 Novartis Ag Ckit antibody drug conjugates
CA2965689C (en) 2014-11-07 2022-03-22 Eleven Biotherapeutics, Inc. Improved il-6 antibodies
US11031102B2 (en) 2014-11-17 2021-06-08 Alexion Pharmaceuticals, Inc. Risk evaluation and management strategy involving patient follow-ups relating to the use or discontinuation of a complement inhibitor
US20160168237A1 (en) 2014-12-12 2016-06-16 Alexion Pharmaceuticals, Inc. Method for treating a complement mediated disorder caused by an infectious agent in a patient
SI3233921T1 (en) 2014-12-19 2022-01-31 Chugai Seiyaku Kabushiki Kaisha Anti-c5 antibodies and methods of use
CN113956354A (en) 2015-01-22 2022-01-21 中外制药株式会社 Combinations and methods of use of two or more anti-C5 antibodies
US20180311299A1 (en) * 2015-05-01 2018-11-01 Alexion Pharmaceuticals, Inc. Efficacy of an anti-c5 antibody in the prevention of antibody mediated rejection in sensitized recipients of a kidney transplant
ES2913530T3 (en) 2015-08-12 2022-06-02 Novartis Ag Methods for treating ophthalmic disorders
WO2017035405A1 (en) 2015-08-26 2017-03-02 Achillion Pharmaceuticals, Inc. Amino compounds for treatment of immune and inflammatory disorders
WO2017035401A1 (en) 2015-08-26 2017-03-02 Achillion Pharmaceuticals, Inc. Amide compounds for treatment of immune and inflammatory disorders
US11203634B2 (en) 2015-10-07 2021-12-21 Alexion Pharmaceuticals, Inc. Methods of treating age-related macular degeneration in a patient comprising administering an anti-C5a antibody
TW201718014A (en) 2015-10-12 2017-06-01 諾華公司 Use of C5 inhibitors in Transplant Associated Microangiopathy
US20180311345A1 (en) 2015-10-30 2018-11-01 Alexion Pharmaceuticals, Inc. A method of inhibiting exacerbations of t cell-mediated allograft vasculopathy
MX2018007144A (en) * 2015-12-18 2018-11-29 Chugai Pharmaceutical Co Ltd Anti-c5 antibodies and methods of use.
UY37030A (en) * 2015-12-18 2017-07-31 Novartis Ag ANTIBODIES DIRECTED TO CD32B AND METHODS OF USE OF THE SAME
US20190023775A1 (en) 2016-01-11 2019-01-24 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treatment
JP2019517473A (en) 2016-05-27 2019-06-24 アレクシオン ファーマシューティカルズ インコーポレイテッドＡｌｅｘｉｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． Methods for the treatment of refractory systemic myasthenia gravis
JP2019521105A (en) * 2016-06-07 2019-07-25 ノバルティス アーゲー Anti-C5 antibody for treating a patient with complement C5 polymorphism
RU2018146847A (en) * 2016-06-07 2020-07-09 Новартис Аг TESIDOLUMAB FOR USE IN TREATMENT OF TRANSPLANT REJECTION
RU2018146778A (en) 2016-06-07 2020-07-09 Новартис Аг ANTIBODY DOSING MODE TO C5
TW202337903A (en) * 2016-06-17 2023-10-01 日商中外製藥股份有限公司 Anti-c5 antibodies and methods of use
CA3026050A1 (en) 2016-08-05 2018-02-08 Chugai Seiyaku Kabushiki Kaisha Composition for prophylaxis or treatment of il-8 related diseases
WO2018071624A1 (en) 2016-10-12 2018-04-19 Alexion Pharmaceuticals, Inc. Efficacy of an anti-c5 antibody in the prevention of antibody mediated rejection in sensitized recipients of a kidney transplant
EP3526248A4 (en) 2016-10-17 2020-07-08 Musc Foundation for Research Development Compositions and methods for treating central nervous system injury
ES2848206T3 (en) 2016-10-19 2021-08-05 Alexion Pharma Inc A method of quantifying unbound C5 in a sample
WO2018081400A1 (en) 2016-10-27 2018-05-03 Alexion Pharmaceuticals Inc. Assay for c5b-9 deposition in complement-associated disorders
EA039858B1 (en) * 2016-11-15 2022-03-21 Ридженерон Фармасьютикалз, Инк. Anti-c5 antibodies and uses thereof
PL3577135T3 (en) 2017-01-31 2023-12-04 Chugai Seiyaku Kabushiki Kaisha A pharmaceutical composition for use in the treatment or prevention of c5-related diseases
AU2018227849B2 (en) 2017-03-01 2022-04-28 Achillion Pharmaceuticals, Inc. Aryl, heteroary, and heterocyclic pharmaceutical compounds for treatment of medical disorders
WO2018160891A1 (en) 2017-03-01 2018-09-07 Achillion Pharmaceutical, Inc. Pharmaceutical compounds for treatment of medical disorders
BR112019018429A2 (en) 2017-03-06 2020-04-14 Univ Pennsylvania antibody, methods for treating an individual's complement-mediated disease or disorder and for reducing the activity of an individual's complement system, and, cell
JP2020517605A (en) 2017-04-19 2020-06-18 アレクシオン ファーマシューティカルズ， インコーポレイテッド Efficacy of anti-C5 antibody in preventing antibody-mediated rejection in sensitized kidney transplant recipients
US11459382B2 (en) 2017-05-22 2022-10-04 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-C5 antibodies for treatment of protein-losing enteropathy in patients
CN117327187A (en) 2017-07-11 2024-01-02 亚力兄制药公司 Polypeptide binding complement component C5 or serum albumin and fusion protein thereof
JP2021501134A (en) 2017-10-26 2021-01-14 アレクシオン ファーマシューティカルズ， インコーポレイテッド Dosage and administration of anti-C5 antibody for the treatment of paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic urinary syndrome (aHUS)
CA3081801C (en) 2017-11-29 2022-12-20 F. Hoffman-La Roche Ag Target interference suppressed anti-drug antibody assay
EP3802593A1 (en) 2018-05-31 2021-04-14 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treatment of paroxysmal nocturnal hemoglobinuria (pnh) in pediatric patients
US20210332147A1 (en) 2018-06-04 2021-10-28 Alexion Pharmaceuticals, Inc. DOSAGE AND ADMINISTRATION OF ANTI-C5 ANTIBODIES FOR TREATMENT OF ATYPICAL HEMOLYTIC UREMIC SYNDROME (aHUS) IN PEDIATRIC PATIENTS
EP3810269A2 (en) * 2018-06-19 2021-04-28 Atarga, LLC Antibody molecules to complement component 5 and uses thereof
EP3814373A1 (en) 2018-06-28 2021-05-05 Alexion Pharmaceuticals, Inc. Methods of producing anti-c5 antibodies
JP6672516B2 (en) 2018-08-01 2020-03-25 中外製薬株式会社 Pharmaceutical composition for treating or preventing C5-related disease and method for treating or preventing C5-related disease
KR20210057086A (en) 2018-09-06 2021-05-20 아칠리온 파르마세우티칼스 인코포레이티드 The morphological form of Danicopan
WO2020051532A2 (en) 2018-09-06 2020-03-12 Achillion Pharmaceuticals, Inc. Macrocyclic compounds for the treatment of medical disorders
US20220056115A1 (en) 2018-09-17 2022-02-24 Kyoto University Administration of an anti-c5 agent for treatment of hepatic injury or failure
US20200095307A1 (en) 2018-09-21 2020-03-26 Alexion Pharmaceuticals, Inc. Compositions and methods for the treatment of neuromyelitis optica
AU2019346464A1 (en) 2018-09-25 2021-04-08 Achillion Pharmaceuticals, Inc. Morphic forms of complement factor D inhibitors
US20210388070A1 (en) 2018-10-30 2021-12-16 Alexion Pharmaceuticals, Inc. Co-administration of a hyaluronidase and anti-c5 antibody for treatment of complement-associated conditions
EP3873602B1 (en) 2018-10-30 2023-12-06 Alexion Pharmaceuticals, Inc. Subcutaneous dosage and administration of anti-c5 antibodies for treatment of paroxysmal nocturnal hemoglobinuria (pnh)
WO2020106724A1 (en) 2018-11-20 2020-05-28 Alexion Pharmaceuticals, Inc. Methods for treatment of refractory generalized myasthenia gravis in pediatric patients
GB2583560A (en) 2018-12-11 2020-11-04 Admirx Inc Fusion protein constructs for complement associated disease
US20220235121A1 (en) 2019-01-25 2022-07-28 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treatment of atypical hemolytic uremic syndrome (ahus)
JP2022520107A (en) 2019-02-14 2022-03-28 アレクシオン ファーマシューティカルズ， インコーポレイテッド Dosage and administration of anti-C5 antibody for the treatment of systemic myasthenia gravis
US10963841B2 (en) 2019-03-27 2021-03-30 On Time Staffing Inc. Employment candidate empathy scoring system
US10728443B1 (en) 2019-03-27 2020-07-28 On Time Staffing Inc. Automatic camera angle switching to create combined audiovisual file
US20220227851A1 (en) 2019-05-24 2022-07-21 Alexion Pharmaceuticals, Inc. Methods of treating vitiligo using an anti-c5 antibody
AU2020319677A1 (en) 2019-07-31 2022-01-06 F. Hoffmann-La Roche Ag Dosage and administration regimen for the treatment or prevention of C5-related diseases by the use of the anti-C5 antibody Crovalimab
EP4003409A1 (en) 2019-07-31 2022-06-01 F. Hoffmann-La Roche AG Dosage and administration regimen for the treatment or prevention of c5-related diseases by the use of the anti-c5 antibody crovalimab
US20220259305A1 (en) 2019-08-05 2022-08-18 Alexion Pharmaceuticals, Inc. Methods for treatment of refractory generalized myasthenia gravis with eculizumab
CN116782876A (en) 2019-08-16 2023-09-19 瑞泽恩制药公司 High concentration anti-C5 formulations
AU2019468121A1 (en) 2019-09-27 2022-05-12 Volution Immuno Pharmaceuticals Sa Method of treatment of hematopoietic stem cell transplant associated thrombotic microangiopathy (HSCT-TMA)
WO2021067526A1 (en) 2019-10-02 2021-04-08 Alexion Pharmaceuticals, Inc. Complement inhibitors for treating drug-induced complement-mediated response
CA3153195A1 (en) 2019-10-25 2021-04-29 John Davis Dosing regimens for treating or preventing c5-associated diseases
US11127232B2 (en) 2019-11-26 2021-09-21 On Time Staffing Inc. Multi-camera, multi-sensor panel data extraction system and method
BR112022011280A2 (en) 2019-12-09 2022-09-06 Alexion Pharma Inc ANTI-CD5 ANTIBODY FOR THE TREATMENT OF NEUROMYELITIS OPTIC SPECTRUM DYSFUNCTION
US20230043576A1 (en) 2019-12-23 2023-02-09 Alexion Pharmaceuticals, Inc. Methods of treating pregnancy-associated atypical hemolytic uremic syndrome using an anti-c5 antibody
US11023735B1 (en) 2020-04-02 2021-06-01 On Time Staffing, Inc. Automatic versioning of video presentations
WO2021211940A1 (en) 2020-04-16 2021-10-21 Assistance Publique, Hopitaux De Paris Methods for treating a complement mediated disorder caused by viruses
KR20230009431A (en) 2020-05-12 2023-01-17 알렉시온 파마슈티칼스, 인코포레이티드 Use of a complement factor D inhibitor alone or in combination with an anti-C5 antibody for the treatment of paroxysmal nocturnal hemoglobinuria
US20230349887A1 (en) 2020-05-15 2023-11-02 Alexion Pharmaceuticals, Inc. Method of using extracellular vesicles to detect complement activation, and uses thereof to assess and/or monitor treatment of a complement-mediated disease
CN113754763B (en) * 2020-06-05 2024-03-08 天辰生物医药(苏州)有限公司 Isolated antigen binding proteins and uses thereof
WO2021254926A1 (en) 2020-06-16 2021-12-23 F. Hoffmann-La Roche Ag Method for determining the free antigen of an antibody in a sample
CN116194478A (en) 2020-06-24 2023-05-30 阿雷克森制药公司 Treatment of complement-associated disorders by Subcutaneous (SC) administration of anti-C5 antibodies
EP4178674A1 (en) 2020-07-09 2023-05-17 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treating paroxysmal nocturnal hemoglobinuria (pnh) in pediatric patients
US20230357438A1 (en) * 2020-07-15 2023-11-09 Biosion Inc. Antibodies binding c5 and uses thereof
EP4196161A1 (en) 2020-08-13 2023-06-21 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treating hematopoietic stem cell transplant-associated thrombotic microangiopathy (hsct-tma)
US11144882B1 (en) 2020-09-18 2021-10-12 On Time Staffing Inc. Systems and methods for evaluating actions over a computer network and establishing live network connections
WO2022060931A1 (en) 2020-09-21 2022-03-24 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treating c5-mediated glomerular nephritis (gn), including lupus nephritis (ln) and/or iga nephropathy (igan)
JP2023548046A (en) 2020-10-23 2023-11-15 アレクシオン ファーマシューティカルズ， インコーポレイテッド Method of treating patients with complement disorders using anti-C5 antibodies
CN114149501B (en) * 2020-12-11 2023-05-26 天士力生物医药股份有限公司 anti-C5 antibodies and uses thereof
JP2024504366A (en) 2021-01-22 2024-01-31 アレクシオン ファーマシューティカルズ， インコーポレイテッド Method of treating complement-mediated thrombotic microangiopathy using anti-C5 antibodies
WO2022260995A2 (en) * 2021-06-07 2022-12-15 Janssen Biotech, Inc. Materials and methods for differentiating creb regulated transcription coactivator 3
WO2022265915A1 (en) 2021-06-14 2022-12-22 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treating dermatomyositis (dm)
WO2023287991A1 (en) 2021-07-14 2023-01-19 Alexion Pharmaceuticals, Inc. Dosage and administration of anti-c5 antibodies for treatment of myasthenia gravis
US11727040B2 (en) 2021-08-06 2023-08-15 On Time Staffing, Inc. Monitoring third-party forum contributions to improve searching through time-to-live data assignments
WO2023023220A1 (en) 2021-08-20 2023-02-23 Alexion Pharmaceuticals, Inc. Methods for treating sickle cell disease or beta thalassemia using a complement alternative pathway inhibitor
US11423071B1 (en) 2021-08-31 2022-08-23 On Time Staffing, Inc. Candidate data ranking method using previously selected candidate data
US20230203176A1 (en) 2021-09-17 2023-06-29 Novartis Ag Methods For Prevention Of Graft Rejection In Xenotransplantation
WO2023077053A2 (en) 2021-10-28 2023-05-04 Regeneron Pharmaceuticals, Inc. Crispr/cas-related methods and compositions for knocking out c5
US11907652B2 (en) 2022-06-02 2024-02-20 On Time Staffing, Inc. User interface and systems for document creation
WO2024054436A1 (en) 2022-09-06 2024-03-14 Alexion Pharmaceuticals, Inc. Diagnostic and prognostic biomarker profiles in patients with hematopoietic stem cell transplant-associated thrombotic microangiopathy (hsct-tma)
WO2024054408A1 (en) 2022-09-06 2024-03-14 Alexion Pharmaceuticals, Inc. Supplemental dosage and administration of anti-c5 antibodies for treating hematopoietic stem cell transplant-associated thrombotic microangiopathy (hsct-tma)

Family Cites Families (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
EP0154316B1 (en) 1984-03-06 1989-09-13 Takeda Chemical Industries, Ltd. Chemically modified lymphokine and production thereof
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4881175A (en) 1986-09-02 1989-11-14 Genex Corporation Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
DE3883899T3 (en) 1987-03-18 1999-04-22 Sb2 Inc CHANGED ANTIBODIES.
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5258498A (en) 1987-05-21 1993-11-02 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
JPH02500329A (en) 1987-05-21 1990-02-08 クリエイテイブ・バイオマリキユールズ・インコーポレーテツド Targeted multifunctional protein
US5132405A (en) 1987-05-21 1992-07-21 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5091513A (en) 1987-05-21 1992-02-25 Creative Biomolecules, Inc. Biosynthetic antibody binding sites
US5677425A (en) 1987-09-04 1997-10-14 Celltech Therapeutics Limited Recombinant antibody
US5336603A (en) 1987-10-02 1994-08-09 Genentech, Inc. CD4 adheson variants
US5506247A (en) 1988-04-15 1996-04-09 T Cell Sciences, Inc. Compounds that inhibit complement and/or suppress immune activity
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
KR900005995A (en) 1988-10-31 1990-05-07 우메모또 요시마사 Modified Interleukin-2 and Method of Making the Same
JP2989002B2 (en) 1988-12-22 1999-12-13 キリン―アムジエン・インコーポレーテツド Chemically modified granulocyte colony stimulating factor
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5112946A (en) 1989-07-06 1992-05-12 Repligen Corporation Modified pf4 compositions and methods of use
FR2650598B1 (en) 1989-08-03 1994-06-03 Rhone Poulenc Sante DERIVATIVES OF ALBUMIN WITH THERAPEUTIC FUNCTION
WO1991006570A1 (en) 1989-10-25 1991-05-16 The University Of Melbourne HYBRID Fc RECEPTOR MOLECULES
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
JP3068180B2 (en) 1990-01-12 2000-07-24 アブジェニックス インコーポレイテッド Generation of heterologous antibodies
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5349053A (en) 1990-06-01 1994-09-20 Protein Design Labs, Inc. Chimeric ligand/immunoglobulin molecules and their uses
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
WO1993012227A1 (en) 1991-12-17 1993-06-24 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
KR100272077B1 (en) 1990-08-29 2000-11-15 젠팜인터내셔날,인코포레이티드 Transgenic non-human animals capable of producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
DK0517895T3 (en) 1990-12-14 1997-04-07 Univ California Chimeric chains for receptor-linked signal transduction pathways
ES2313867T3 (en) 1991-12-02 2009-03-16 Medical Research Council ANTI-AUTO ANTIBODY PRODUCTION OF ANTIBODY SEGMENT REPERTORIES EXPRESSED ON THE PAYMENT SURFACE.
CA2507749C (en) 1991-12-13 2010-08-24 Xoma Corporation Methods and materials for preparation of modified antibody variable domains and therapeutic uses thereof
US5622929A (en) 1992-01-23 1997-04-22 Bristol-Myers Squibb Company Thioether conjugates
FR2686899B1 (en) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa NOVEL BIOLOGICALLY ACTIVE POLYPEPTIDES, THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM.
FR2686901A1 (en) 1992-01-31 1993-08-06 Rhone Poulenc Rorer Sa NOVEL ANTITHROMBOTIC POLYPEPTIDES, THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM.
US5714350A (en) 1992-03-09 1998-02-03 Protein Design Labs, Inc. Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region
US5447851B1 (en) 1992-04-02 1999-07-06 Univ Texas System Board Of Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells
EP0640094A1 (en) 1992-04-24 1995-03-01 The Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
EP1498427B1 (en) 1992-08-21 2009-12-16 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
JPH08509612A (en) 1993-04-26 1996-10-15 ジェンファーム インターナショナル インコーポレイテッド Transgenic non-human animal capable of producing heterologous antibody
WO1994029351A2 (en) 1993-06-16 1994-12-22 Celltech Limited Antibodies
SE9400088D0 (en) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6074642A (en) * 1994-05-02 2000-06-13 Alexion Pharmaceuticals, Inc. Use of antibodies specific to human complement component C5 for the treatment of glomerulonephritis
AU3382595A (en) 1994-07-29 1996-03-04 Smithkline Beecham Corporation Novel compounds
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
US6121022A (en) 1995-04-14 2000-09-19 Genentech, Inc. Altered polypeptides with increased half-life
WO1997028276A1 (en) 1996-02-02 1997-08-07 Stichting Onderzoeksbeleid Cardiopulmonale Chirurgie Measurement of complement activation by biomaterials by means of complement convertase cleavage of peptide substrates
AU728657B2 (en) 1996-03-18 2001-01-18 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
US5843449A (en) 1996-03-25 1998-12-01 Akzo Nobel N.V. Proteins and novel peptides derived from autoantigen for use in immunotherapy of autoimmune diseases
DE69738670D1 (en) 1996-10-28 2008-06-19 Inst Suisse De Rech S Experime METHOD FOR THE OLIGOMERIZATION OF PEPTIDES
WO1998023289A1 (en) 1996-11-27 1998-06-04 The General Hospital Corporation MODULATION OF IgG BINDING TO FcRn
US6277375B1 (en) 1997-03-03 2001-08-21 Board Of Regents, The University Of Texas System Immunoglobulin-like domains with increased half-lives
GB2339430A (en) 1997-05-21 2000-01-26 Biovation Ltd Method for the production of non-immunogenic proteins
DE69827507T2 (en) 1997-06-11 2006-03-09 Borean Pharma A/S TRIMERIZING MODULE
WO1998056915A2 (en) 1997-06-12 1998-12-17 Research Corporation Technologies, Inc. Artificial antibody polypeptides
DE19742706B4 (en) 1997-09-26 2013-07-25 Pieris Proteolab Ag lipocalin muteins
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
JP4334141B2 (en) 1998-04-20 2009-09-30 グリカート バイオテクノロジー アクチェンゲゼルシャフト Engineering glycosylation of antibodies to improve antibody-dependent cytotoxicity
US6015803A (en) 1998-05-04 2000-01-18 Wirostko; Emil Antibiotic treatment of age-related macular degeneration
US6818418B1 (en) 1998-12-10 2004-11-16 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
KR101077001B1 (en) 1999-01-15 2011-10-26 제넨테크, 인크. Polypeptide Variants with Altered Effector Function
EP1804064A1 (en) 1999-02-19 2007-07-04 University Of Iowa Research Foundation Diagnostics and therapeutics for macular degeneration
US7108982B1 (en) * 1999-02-19 2006-09-19 University Of Iowa Research Foundation Diagnostics and the therapeutics for macular degeneration
AU3704500A (en) 1999-02-19 2001-01-22 University Of Iowa Research Foundation, The Diagnostics and therapeutics for arterial wall disruptive disorders
AU3672800A (en) 1999-04-09 2000-11-14 Kyowa Hakko Kogyo Co. Ltd. Method for controlling the activity of immunologically functional molecule
DE19932688B4 (en) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design of beta-sheet proteins of gamma-II-crystalline antibody-like
PT1144607E (en) 1999-07-20 2009-04-22 Morphosys Ag Methods for displaying (poly)peptides/proteins on bacteriophage particles via disulfide bonds
IL148079A0 (en) 1999-08-24 2002-09-12 Medarex Inc Human ctla-4 antibodies and compositions containing the same
WO2001014558A1 (en) * 1999-08-26 2001-03-01 Morphosys Ag Human antibodies or fragments thereof binding to hla-cw6
US7011952B2 (en) * 2000-02-22 2006-03-14 University Of Iowa Research Foundation Diagnostics and therapeutics for macular degeneration-related disorders
JP2004506407A (en) 2000-04-12 2004-03-04 ヒューマン　ゲノム　サイエンシズ　インコーポレイテッド Albumin fusion protein
AU2001261103B2 (en) * 2000-04-29 2007-08-23 University Of Iowa Research Foundation Diagnostics and therapeutics for macular degeneration-related disorders
WO2001084144A1 (en) 2000-05-04 2001-11-08 Jussi Halleen Method for in vitro screening of drug candidates useful for the prevention of bone resorption
CA2441903C (en) 2000-05-26 2012-07-31 National Research Council Of Canada Single-domain brain-targeting antibody fragments derived from llama antibodies
WO2002030985A2 (en) * 2000-10-10 2002-04-18 Tanox, Inc. Inhibition of complement c5 activation for the treatment and prevention of delayed xenograft or acute vascular rejection
CN1487996B (en) 2000-11-30 2010-06-16 米德列斯公司 Transgenic transchromosomal rodents for making human antibodies
JP4406206B2 (en) * 2001-04-24 2010-01-27 バイエル コーポレーション Human TIMP-1 antibody
US20050048512A1 (en) 2001-04-26 2005-03-03 Avidia Research Institute Combinatorial libraries of monomer domains
US20040175756A1 (en) 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US20050053973A1 (en) 2001-04-26 2005-03-10 Avidia Research Institute Novel proteins with targeted binding
EP1421203A4 (en) 2001-05-17 2005-06-01 Diversa Corp Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
WO2003035835A2 (en) 2001-10-25 2003-05-01 Genentech, Inc. Glycoprotein compositions
US20030157579A1 (en) 2002-02-14 2003-08-21 Kalobios, Inc. Molecular sensors activated by disinhibition
US7335478B2 (en) 2002-04-18 2008-02-26 Kalobios Pharmaceuticals, Inc. Reactivation-based molecular interaction sensors
US20040110226A1 (en) 2002-03-01 2004-06-10 Xencor Antibody optimization
ITMI20021527A1 (en) * 2002-07-11 2004-01-12 Consiglio Nazionale Ricerche C5 COMPONENT ANTIBODIES OF COMPLEMENT AND THEIR USE
US7816497B2 (en) 2002-10-30 2010-10-19 University Of Kentucky Compositions and methods for inhibiting drusen complement components C3a and C5a for the treatment of age-related macular degeneration
WO2004072266A2 (en) 2003-02-13 2004-08-26 Kalobios Inc. Antibody affinity engineering by serial epitope-guided complementarity replacement
DE10324447A1 (en) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generation of artificial binding proteins based on ubiquitin
US7981843B2 (en) 2004-01-20 2011-07-19 Kalobios Pharmaceuticals, Inc. Antibody specificity transfer using minimal essential binding determinants
US20050169921A1 (en) * 2004-02-03 2005-08-04 Leonard Bell Method of treating hemolytic disease
US20070116710A1 (en) 2004-02-03 2007-05-24 Leonard Bell Methods of treating hemolytic anemia
US20060008844A1 (en) 2004-06-17 2006-01-12 Avidia Research Institute c-Met kinase binding proteins
EP1942922A4 (en) 2005-11-04 2011-03-30 Genentech Inc Use of complement pathway inhibitors to treat ocular diseases
US20070196367A1 (en) * 2006-02-22 2007-08-23 Valentin Dinu Methods of preventing and treating Alzheimer's disease, age related macular degeneration and other diseases involving extra-cellular debris through the inhibition of the complement system
CA3022097C (en) 2006-03-15 2020-10-27 Alexion Pharmaceuticals, Inc. Treatment of paroxysmal nocturnal hemoglobinuria patients by an inhibitor of complement
ES2541142T3 (en) * 2008-08-05 2015-07-16 Novartis Ag Compositions and methods for antibodies directed against complement C5 protein
CA2859493A1 (en) * 2011-12-21 2013-06-27 Novartis Ag Compositions and methods for antibodies targeting factor p
US20170073754A1 (en) * 2014-02-07 2017-03-16 Novartis Ag Impact of genetic factors on disease progression and response to anti-c5 antibody in geographic atrophy
MA45235A (en) * 2016-06-14 2019-04-17 Regeneron Pharma ANTI-C5 ANTIBODIES AND THEIR USES

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10407496B2 (en) * 2008-08-05 2019-09-10 Novartis Ag Compositions and methods for antibodies targeting complement protein C5
WO2017218515A1 (en) 2016-06-14 2017-12-21 Regeneron Pharmaceuticals, Inc. Anti-c5 antibodies and uses thereof
US10633434B2 (en) 2016-06-14 2020-04-28 Regeneron Pharmaceuticals, Inc. Anti-C5 antibodies
US11479602B2 (en) 2016-06-14 2022-10-25 Regeneren Pharmaceuticals, Inc. Methods of treating C5-associated diseases comprising administering anti-C5 antibodies
US11492392B2 (en) 2016-06-14 2022-11-08 Regeneran Pharmaceuticals, Inc. Polynucleotides encoding anti-C5 antibodies
WO2019118556A1 (en) 2017-12-13 2019-06-20 Regeneron Pharmaceuticals, Inc. Anti-c5 antibody combinations and uses thereof
US11365265B2 (en) 2017-12-13 2022-06-21 Regeneron Pharmaceuticals, Inc. Anti-C5 antibody combinations and uses thereof

Also Published As

Publication number Publication date
EA027575B1 (en) 2017-08-31
EP2815766B1 (en) 2017-07-05
TWI417107B (en) 2013-12-01
PE20110225A1 (en) 2011-04-05
US8883158B2 (en) 2014-11-11
KR20110038173A (en) 2011-04-13
EP2328616A1 (en) 2011-06-08
CN102170906B (en) 2014-07-30
TW201010723A (en) 2010-03-16
US20170260260A1 (en) 2017-09-14
JP2016020345A (en) 2016-02-04
JO3118B1 (en) 2017-09-20
JP6224657B2 (en) 2017-11-01
EP2328616B1 (en) 2015-04-29
CO6351750A2 (en) 2011-12-20
JP5815403B2 (en) 2015-11-17
CN102170906A (en) 2011-08-31
SV2011003834A (en) 2011-03-23
US20130064836A1 (en) 2013-03-14
KR101463296B1 (en) 2014-11-27
HRP20150799T1 (en) 2015-09-11
CR20110035A (en) 2011-03-08
CR20160206A (en) 2016-07-11
SG10201405377XA (en) 2014-12-30
US20100034809A1 (en) 2010-02-11
EP2815766A1 (en) 2014-12-24
HK1157663A1 (en) 2012-07-06
WO2010015608A1 (en) 2010-02-11
CU20110029A7 (en) 2012-06-21
NZ590634A (en) 2012-07-27
AU2009279197A1 (en) 2010-02-11
HK1202269A1 (en) 2015-09-25
UA105009C2 (en) 2014-04-10
HK1203156A1 (en) 2015-10-23
HUE026179T2 (en) 2016-05-30
PT2328616E (en) 2015-08-26
TW201350130A (en) 2013-12-16
IL210768A0 (en) 2011-03-31
CA2732782C (en) 2019-02-26
US8241628B2 (en) 2012-08-14
MX356218B (en) 2018-05-18
DK2328616T3 (en) 2015-07-20
NZ600622A (en) 2013-12-20
AR072897A1 (en) 2010-09-29
AU2009279197B2 (en) 2013-12-12
BRPI0916668A2 (en) 2019-10-15
MY159396A (en) 2016-12-30
CN104177495B (en) 2017-07-28
US10407496B2 (en) 2019-09-10
HK1156226A1 (en) 2012-06-08
MX2011001371A (en) 2011-06-16
CL2011000233A1 (en) 2012-03-16
CN104177495A (en) 2014-12-03
HN2011000357A (en) 2014-07-31
SI2328616T1 (en) 2015-08-31
US20130022615A1 (en) 2013-01-24
RS54113B1 (en) 2015-12-31
EP2837388A1 (en) 2015-02-18
JP2011529700A (en) 2011-12-15
MA32535B1 (en) 2011-08-01
EA201100300A1 (en) 2011-12-30
CA2732782A1 (en) 2010-02-11
ZA201100375B (en) 2011-10-26
PL2328616T3 (en) 2015-10-30
ES2541142T3 (en) 2015-07-16
CU23978B1 (en) 2014-01-29
ECSP11010805A (en) 2011-03-31
IL210768B (en) 2018-02-28
ES2643411T3 (en) 2017-11-22

Similar Documents

Publication Publication Date Title
US10407496B2 (en) Compositions and methods for antibodies targeting complement protein C5
US10865237B2 (en) Nucleic acids encoding anti-Factor P antibodies
US20100291106A1 (en) Compositions and methods for antibodies targeting complement protein c3b
AU2014201433B2 (en) Compositions and methods for antibodies targeting complement protein C5
BRPI0916668B1 (en) ISOLATED MONOCLONAL ANTIBODY, PHARMACEUTICAL COMPOSITION, ISOLATED NUCLEIC ACID AND VECTOR

Legal Events

Date Code Title Description
AS Assignment

Owner name: NOVARTIS AG, SWITZERLAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:DIEFENBACH-STREIBER, BEATE;EBERTH, ADINA;GUILD, BRAYDON CHARLES;AND OTHERS;SIGNING DATES FROM 20090819 TO 20090909;REEL/FRAME:035921/0627

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION