US20150344912A1 - Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof - Google Patents

Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof Download PDF

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Publication number
US20150344912A1
US20150344912A1 US14/685,510 US201514685510A US2015344912A1 US 20150344912 A1 US20150344912 A1 US 20150344912A1 US 201514685510 A US201514685510 A US 201514685510A US 2015344912 A1 US2015344912 A1 US 2015344912A1
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United States
Prior art keywords
seq
cas9
guide rna
nucleic acid
target
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US14/685,510
Inventor
Jin-soo Kim
Seung Woo Cho
Sojung Kim
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Toolgen Inc
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Toolgen Inc
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Publication date
Family has litigation
Priority to US201261717324P priority Critical
Priority to US201361803599P priority
Priority to US201361837481P priority
Priority to PCT/KR2013/009488 priority patent/WO2014065596A1/en
Application filed by Toolgen Inc filed Critical Toolgen Inc
Priority to US14/685,510 priority patent/US20150344912A1/en
Assigned to TOOLGEN INCORPORATED reassignment TOOLGEN INCORPORATED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHO, SEUNG WOO, KIM, JONG MIN, KIM, SOJUNG, KIM, JIN-SOO, KIM, Seokjoong
Publication of US20150344912A1 publication Critical patent/US20150344912A1/en
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50544909&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=US20150344912(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • C—<