US20110086403A1 - Photoinhibition of microbial nitrification in potable water - Google Patents
Photoinhibition of microbial nitrification in potable water Download PDFInfo
- Publication number
- US20110086403A1 US20110086403A1 US12/879,844 US87984410A US2011086403A1 US 20110086403 A1 US20110086403 A1 US 20110086403A1 US 87984410 A US87984410 A US 87984410A US 2011086403 A1 US2011086403 A1 US 2011086403A1
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- United States
- Prior art keywords
- water
- nitrification
- uva
- lamps
- microbial nitrification
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
Definitions
- the present invention generally relates to apparatus and methods for controlling microbial nitrification and more specifically, to apparatus and methods for providing a dose of ultraviolet-A (UVA)/Visible light in covered drinking water storage facilities to control nitrification.
- UVA ultraviolet-A
- Nitrifying microbes may produce nitrites and nitrates through the oxidation of ammonia produced by the degradation of chloramine disinfectant.
- Nitrate is the cause of methemoglobinemia (blue-baby syndrome) in infants.
- a system for the photo-inhibition of microbial nitrification of potable water comprises a tank for holding water; and at least one lamp irradiating light into the water.
- FIG. 1 is a graph of incident irradiance over a spectrum between 250 and 700 nm for a variety of lamps;
- FIG. 2 a graph showing the incident irradiance of an exemplary blue-blocking blacklight lamp (BLB) through various lenses;
- FIG. 3 shows a lighting design according to an embodiment of the present invention
- FIG. 4 is a schematic drawing of a water storage tank having a microbial nitrification photo-inhibition system according to an exemplary embodiment of the present invention
- FIG. 5 is a schematic drawing of a water storage tank having a microbial nitrification photo-inhibition system according to an alternate exemplary embodiment of the present invention
- FIG. 6 is an engineering drawing of an exemplary storage tank used in the various studies described below;
- FIG. 7 is a bar graph showing UVA intensity at various points, horizontally, at 4 feet above ground level (AGL) in air in the storage tank of FIG. 6 ;
- FIG. 8 is a bar graph showing UVA intensity at various points, vertically, at 28′′ from the wall of the storage tank of FIG. 6 .
- FIG. 9 is a bar graph showing UVA intensity at various points, vertically, at 28′′ from the wall of the storage tank of FIG. 6 , over a three day period;
- FIG. 10 is a graph describing results of a bottle test with and without the microbial nitrification photo-inhibition system of the present invention.
- FIG. 11 a is a chart describing the distribution of UVA intensity with depth at high water, including estimates of the time to half-inhibition for each flux.
- FIG. 11 b is a chart describing the distribution of UVA intensity with depth at low water, including estimates of the time to half-inhibition for each flux.
- embodiments of the present invention generally provide an inhibiting dose of UVA/Visible light in covered drinking water storage facilities to prevent nitrification.
- Embodiments of the current invention use UVA/Visible radiation to control bacterial nitrification in an industrial setting.
- an “inhibiting dose” of UVA/Visible light may be radiation at a suitable wavelength and intensity over a specific period of time, typically 24 hours or less, to inhibit enzymes used in nitrifying microbes.
- an inhibiting dose may be radiation having a wavelength between 315 and 430 nanometers (nm) and an incident irradiance of at least 0.01 watts per square meter (W/m 2 ) over a period of 24 hours, typically at least 0.01 W/m 2 .
- the term “photo-inhibition”, with respect to microbial nitrification, refers to not only the prevention of microbial nitrification in water, but also to the treatment of microbial nitrification once such a process has started.
- “reducing” microbial nitrification of water refers not only to lessening the rate and/or degree of microbial nitrification, but also to the elimination of microbial nitrification of water.
- FIG. 1 there is shown a graph of incident irradiance over a spectrum between 250 and 700 nm for a variety of lamps.
- a blue-blocking blacklight lamp was chosen as the lamp to use in the below described experiments. While this specific lamp was chosen for further study, the invention should not be limited to any particular lamp, provided that the lamp may provide an adequate dose to achieve the desired result of inhibiting microbial nitrification.
- fluorescent, incandescent or LED lamps may be useful in various embodiments of the present invention.
- FIG. 2 there is shown a graph of the irradiance of an exemplary blue-blocking blacklight lamp (BLB) through various lenses.
- the emission spectrum of the BLB lamp is shown by the line with the circles. This line should be read against the Y-axis on the left.
- the other three lines show the percent transmission of the BLB emission spectrum through various lenses—a stock lens (triangle-marked line), a UVA transmitting lens (diamond-marked line), and two stacked UVA transmitting lenses (square-marked line).
- the UVA transmitting lenses provided a greater transmittance throughout the entire measured spectrum (335-400 nm).
- FIG. 3 there is shown a lighting design useful in a water storage tank, according to an embodiment of the present invention.
- the lighting design uses a number of light banks 30 , each containing at least one lamp, such as at least one F40T12BLB median bipin fluorescent lamp (not shown).
- the exemplary lighting design of FIG. 3 may be based on comparative scans of warm white lamps with suitable design software.
- the lighting design of FIG. 3 should be taken as an exemplary lighting design and should not be considered as limiting the choice of lighting design useful in the present invention.
- FIG. 4 there is shown a schematic drawing of a water storage tank 40 having a microbial nitrification photo-inhibition system according to the present invention.
- the system may include a plurality of lamps 42 , such as the BLB lamps as described above.
- the lamps may be controlled by a timer 44 .
- the output of the lamps 42 may be measured by at least one light meter 46 .
- a number of light meters 46 may be disposed at various locations above ground level (AGL).
- AGL ground level
- three light meters 46 may be disposed along one side of the water storage tank 40 .
- the lamps 42 are shown above the water level, the lamps 42 may be located at various locations inside the water tank 40 , such as above the water level, submerged in the water within the tank 40 , or floating on top of the water in the tank 40 .
- a water storage tank 50 may be similar to the water storage 40 described above, with the addition of a dimmer 52 for controlling the intensity of the output of the lamps.
- the water storage tank 50 may also include a mixing device 54 for mixing water stored in the tank 50 .
- the mixing device 54 may be controlled by a timer 56 . Control of the various components, such as the lamps, timers, dimmers, mixing devices and the like may be performed on-site or remotely via any known control technique. For example, a Supervisory Control and Data Acquisition (SCADA) system may be used to remotely control the system of the present invention.
- SCADA Supervisory Control and Data Acquisition
- FIG. 6 there is shown an engineering drawing of an exemplary storage tank used in the various studies described below.
- FIG. 7 shows the UVA intensity at various points horizontally at 4 feet above ground level (AGL) in air in the storage tank of FIG. 6 .
- FIG. 8 shows the UVA intensity at various points, vertically, at 28′′ from the wall of the storage tank of FIG. 6 .
- FIG. 9 shows showing UVA intensity at various points, vertically, at 28′′ from the wall of the storage tank of FIG. 6 , over a three day period.
- FIG. 10 there is shown a graph describing results of a bottle test with and without the microbial nitrification photo-inhibition system of the present invention.
- three 5-liter pyrex bottles containing 3 liters of water in each, were suspended from two water storage tanks (six 5-liter pyrex bottles in total). The bottles were placed 3.5 feet above ground level in each tank. Nitrification had begun in the water sample prior to filling the pyrex bottles.
- One tank was kept in the dark and one tank was provided with UVA/Visible light according to an embodiment of the present invention.
- the tanks in the dark were of considerably poorer quality compared to those under light.
- FIG. 11 a there is shown a chart describing the distribution of UVA intensity with depth at high water, including estimates of the time to half-inhibition for each flux.
- FIG. 11 b shows a chart describing the distribution of UVA intensity with depth at low water, including estimates of the time to half-inhibition for each flux.
Abstract
A system for the photo-inhibition of microbial nitrification of potable water may include overhead, floating or submerged lamps (fluorescent, incandescent, LED, etc. . . . ) with spectral emission between 315-430 nm, headspace and submerged light meters for measuring the intensity above and below the water surface, a water mixing device to manage full water column dose, and a controller for operating associated lamp and mixer operations. The applied irradiance may typically be equal to or exceed 0.01 W/m2.
Description
- The present invention generally relates to apparatus and methods for controlling microbial nitrification and more specifically, to apparatus and methods for providing a dose of ultraviolet-A (UVA)/Visible light in covered drinking water storage facilities to control nitrification.
- It is well established in the scientific literature that the enzymes used by nitrifying microbes to first oxidize ammonia to nitrite and then nitrite to nitrate are inhibited by UVA and visible light. Potable water is often stored in large water storage tanks. Nitrifying microbes may produce nitrites and nitrates through the oxidation of ammonia produced by the degradation of chloramine disinfectant.
- In covered drinking water storage, nitrification contributes to the loss of disinfectant residual, stimulates the growth of other nuisance microbes and produces nitrite and nitrate, both of which are regulated drinking water contaminants. Nitrate is the cause of methemoglobinemia (blue-baby syndrome) in infants.
- In one aspect of the present invention, a system for the photo-inhibition of microbial nitrification of potable water comprises a tank for holding water; and at least one lamp irradiating light into the water.
- These and other features, aspects and advantages of the present invention will become better understood with reference to the following drawings, description and claims.
-
FIG. 1 is a graph of incident irradiance over a spectrum between 250 and 700 nm for a variety of lamps; -
FIG. 2 a graph showing the incident irradiance of an exemplary blue-blocking blacklight lamp (BLB) through various lenses; -
FIG. 3 shows a lighting design according to an embodiment of the present invention; -
FIG. 4 is a schematic drawing of a water storage tank having a microbial nitrification photo-inhibition system according to an exemplary embodiment of the present invention; -
FIG. 5 is a schematic drawing of a water storage tank having a microbial nitrification photo-inhibition system according to an alternate exemplary embodiment of the present invention; -
FIG. 6 is an engineering drawing of an exemplary storage tank used in the various studies described below; -
FIG. 7 is a bar graph showing UVA intensity at various points, horizontally, at 4 feet above ground level (AGL) in air in the storage tank ofFIG. 6 ; -
FIG. 8 is a bar graph showing UVA intensity at various points, vertically, at 28″ from the wall of the storage tank ofFIG. 6 . -
FIG. 9 is a bar graph showing UVA intensity at various points, vertically, at 28″ from the wall of the storage tank ofFIG. 6 , over a three day period; -
FIG. 10 is a graph describing results of a bottle test with and without the microbial nitrification photo-inhibition system of the present invention; -
FIG. 11 a is a chart describing the distribution of UVA intensity with depth at high water, including estimates of the time to half-inhibition for each flux; and -
FIG. 11 b is a chart describing the distribution of UVA intensity with depth at low water, including estimates of the time to half-inhibition for each flux. - The following detailed description is of the best currently contemplated modes of carrying out exemplary embodiments of the invention. The description is not to be taken in a limiting sense, but is made merely for the purpose of illustrating the general principles of the invention, since the scope of the invention is best defined by the appended claims.
- Various inventive features are described below that can each be used independently of one another or in combination with other features.
- Broadly, embodiments of the present invention generally provide an inhibiting dose of UVA/Visible light in covered drinking water storage facilities to prevent nitrification. Embodiments of the current invention use UVA/Visible radiation to control bacterial nitrification in an industrial setting.
- As used herein, an “inhibiting dose” of UVA/Visible light may be radiation at a suitable wavelength and intensity over a specific period of time, typically 24 hours or less, to inhibit enzymes used in nitrifying microbes. For example, an inhibiting dose may be radiation having a wavelength between 315 and 430 nanometers (nm) and an incident irradiance of at least 0.01 watts per square meter (W/m2) over a period of 24 hours, typically at least 0.01 W/m2.
- As used herein, the term “photo-inhibition”, with respect to microbial nitrification, refers to not only the prevention of microbial nitrification in water, but also to the treatment of microbial nitrification once such a process has started. Similarly, “reducing” microbial nitrification of water refers not only to lessening the rate and/or degree of microbial nitrification, but also to the elimination of microbial nitrification of water.
- Referring to
FIG. 1 , there is shown a graph of incident irradiance over a spectrum between 250 and 700 nm for a variety of lamps. After several tests and exploration of a variety of lamps, a blue-blocking blacklight lamp was chosen as the lamp to use in the below described experiments. While this specific lamp was chosen for further study, the invention should not be limited to any particular lamp, provided that the lamp may provide an adequate dose to achieve the desired result of inhibiting microbial nitrification. For example, fluorescent, incandescent or LED lamps may be useful in various embodiments of the present invention. - Referring to
FIG. 2 , there is shown a graph of the irradiance of an exemplary blue-blocking blacklight lamp (BLB) through various lenses. In this graph, the emission spectrum of the BLB lamp is shown by the line with the circles. This line should be read against the Y-axis on the left. The other three lines show the percent transmission of the BLB emission spectrum through various lenses—a stock lens (triangle-marked line), a UVA transmitting lens (diamond-marked line), and two stacked UVA transmitting lenses (square-marked line). As can be seen, the UVA transmitting lenses provided a greater transmittance throughout the entire measured spectrum (335-400 nm). - Referring now to
FIG. 3 , there is shown a lighting design useful in a water storage tank, according to an embodiment of the present invention. The lighting design uses a number oflight banks 30, each containing at least one lamp, such as at least one F40T12BLB median bipin fluorescent lamp (not shown). The exemplary lighting design ofFIG. 3 may be based on comparative scans of warm white lamps with suitable design software. The lighting design ofFIG. 3 should be taken as an exemplary lighting design and should not be considered as limiting the choice of lighting design useful in the present invention. - Referring to
FIG. 4 , there is shown a schematic drawing of awater storage tank 40 having a microbial nitrification photo-inhibition system according to the present invention. The system may include a plurality oflamps 42, such as the BLB lamps as described above. The lamps may be controlled by atimer 44. The output of thelamps 42 may be measured by at least onelight meter 46. Typically, a number oflight meters 46 may be disposed at various locations above ground level (AGL). For example, as shown inFIG. 4 , threelight meters 46 may be disposed along one side of thewater storage tank 40. While thelamps 42 are shown above the water level, thelamps 42 may be located at various locations inside thewater tank 40, such as above the water level, submerged in the water within thetank 40, or floating on top of the water in thetank 40. - In one exemplary embodiment of the present invention, as shown in
FIG. 5 , awater storage tank 50 may be similar to thewater storage 40 described above, with the addition of adimmer 52 for controlling the intensity of the output of the lamps. Thewater storage tank 50 may also include amixing device 54 for mixing water stored in thetank 50. Themixing device 54 may be controlled by atimer 56. Control of the various components, such as the lamps, timers, dimmers, mixing devices and the like may be performed on-site or remotely via any known control technique. For example, a Supervisory Control and Data Acquisition (SCADA) system may be used to remotely control the system of the present invention. - Referring to
FIG. 6 , there is shown an engineering drawing of an exemplary storage tank used in the various studies described below.FIG. 7 shows the UVA intensity at various points horizontally at 4 feet above ground level (AGL) in air in the storage tank ofFIG. 6 .FIG. 8 shows the UVA intensity at various points, vertically, at 28″ from the wall of the storage tank ofFIG. 6 .FIG. 9 shows showing UVA intensity at various points, vertically, at 28″ from the wall of the storage tank ofFIG. 6 , over a three day period. - Referring now to
FIG. 10 , there is shown a graph describing results of a bottle test with and without the microbial nitrification photo-inhibition system of the present invention. In this study, three 5-liter pyrex bottles, containing 3 liters of water in each, were suspended from two water storage tanks (six 5-liter pyrex bottles in total). The bottles were placed 3.5 feet above ground level in each tank. Nitrification had begun in the water sample prior to filling the pyrex bottles. One tank was kept in the dark and one tank was provided with UVA/Visible light according to an embodiment of the present invention. As can be seen from the graph ofFIG. 10 , after 3 days, the tanks in the dark were of considerably poorer quality compared to those under light. - Referring to
FIG. 11 a, there is shown a chart describing the distribution of UVA intensity with depth at high water, including estimates of the time to half-inhibition for each flux.FIG. 11 b shows a chart describing the distribution of UVA intensity with depth at low water, including estimates of the time to half-inhibition for each flux. - It should be understood, of course, that the foregoing relates to exemplary embodiments of the invention and that modifications may be made without departing from the spirit and scope of the invention as set forth in the following claims.
Claims (2)
1. A system for the photo-inhibition of microbial nitrification of potable water, the system comprising:
a tank for holding water; and
at least one lamp illuminating the water.
2. A method for reducing microbial nitrification of potable water, the method comprising:
contacting the water with radiation having a wavelength between 315 and 430 nm; and
providing a sufficient intensity to contact all of the water at an incident irradiance of at least 0.01 W/m2.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/879,844 US20110086403A1 (en) | 2009-09-11 | 2010-09-10 | Photoinhibition of microbial nitrification in potable water |
US14/010,448 US9327998B2 (en) | 2009-09-11 | 2013-08-26 | Photoinhibition of microbial nitrification in potable water |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US24180009P | 2009-09-11 | 2009-09-11 | |
US12/879,844 US20110086403A1 (en) | 2009-09-11 | 2010-09-10 | Photoinhibition of microbial nitrification in potable water |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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US14/010,448 Continuation US9327998B2 (en) | 2009-09-11 | 2013-08-26 | Photoinhibition of microbial nitrification in potable water |
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US20110086403A1 true US20110086403A1 (en) | 2011-04-14 |
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US12/879,844 Abandoned US20110086403A1 (en) | 2009-09-11 | 2010-09-10 | Photoinhibition of microbial nitrification in potable water |
US14/010,448 Active US9327998B2 (en) | 2009-09-11 | 2013-08-26 | Photoinhibition of microbial nitrification in potable water |
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US14/010,448 Active US9327998B2 (en) | 2009-09-11 | 2013-08-26 | Photoinhibition of microbial nitrification in potable water |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3649493A (en) * | 1969-07-10 | 1972-03-14 | Midwest Research Inst | Process of purifying water with active halogen compound and actini radiations |
US5227053A (en) * | 1990-11-30 | 1993-07-13 | Conventure Corporation | Water purification system |
US5474748A (en) * | 1993-12-03 | 1995-12-12 | Szabo; Louis | Water decontaminating device |
US5591978A (en) * | 1994-07-27 | 1997-01-07 | Alvin Kovalsky | All wavelength ultraviolet intensity monitor |
US6193894B1 (en) * | 1999-06-23 | 2001-02-27 | Brad C. Hollander | Methods and apparatus for disinfecting and sterilizing water in water dispensers using ultraviolet radiation |
US6403030B1 (en) * | 2000-07-31 | 2002-06-11 | Horton, Iii Isaac B. | Ultraviolet wastewater disinfection system and method |
-
2010
- 2010-09-10 US US12/879,844 patent/US20110086403A1/en not_active Abandoned
-
2013
- 2013-08-26 US US14/010,448 patent/US9327998B2/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3649493A (en) * | 1969-07-10 | 1972-03-14 | Midwest Research Inst | Process of purifying water with active halogen compound and actini radiations |
US5227053A (en) * | 1990-11-30 | 1993-07-13 | Conventure Corporation | Water purification system |
US5474748A (en) * | 1993-12-03 | 1995-12-12 | Szabo; Louis | Water decontaminating device |
US5591978A (en) * | 1994-07-27 | 1997-01-07 | Alvin Kovalsky | All wavelength ultraviolet intensity monitor |
US6193894B1 (en) * | 1999-06-23 | 2001-02-27 | Brad C. Hollander | Methods and apparatus for disinfecting and sterilizing water in water dispensers using ultraviolet radiation |
US6403030B1 (en) * | 2000-07-31 | 2002-06-11 | Horton, Iii Isaac B. | Ultraviolet wastewater disinfection system and method |
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US20140054240A1 (en) | 2014-02-27 |
US9327998B2 (en) | 2016-05-03 |
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Owner name: DEPARTMENT OF WATER AND POWER OF THE CITY OF LOS A Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:WHITE, BRIAN NELSON;REEL/FRAME:025567/0399 Effective date: 20101215 |
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