US20100273826A1 - Methods of use and compositions for the diagnosis and treatment of infectious diseases - Google Patents

Methods of use and compositions for the diagnosis and treatment of infectious diseases Download PDF

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US20100273826A1
US20100273826A1 US12/693,252 US69325210A US2010273826A1 US 20100273826 A1 US20100273826 A1 US 20100273826A1 US 69325210 A US69325210 A US 69325210A US 2010273826 A1 US2010273826 A1 US 2010273826A1
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tuberculosis
compositions
treatment
resins
disease
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Marina Nikolaevna Protopopova
Elena Bogatcheva
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Sequella Inc
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/185Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19Carboxylic acids, e.g. valproic acid
    • A61K31/195Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A61K31/198Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445Non condensed piperidines, e.g. piperocaine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • A61P31/06Antibacterial agents for tuberculosis

Definitions

  • the present invention relates to methods and compositions for treating disease caused by microorganisms, particularly tuberculosis.
  • the present invention also relates to methods and compositions having improved anti-mycobacterial activity, namely compositions comprising novel substituted ethylene diamine compounds.
  • Mycobacterial infections often manifest as diseases such as tuberculosis.
  • Human infections caused by mycobacteria have been widespread since ancient times, and tuberculosis remains a leading cause of death today.
  • mycobacterial diseases still constitute a leading cause of morbidity and mortality in countries with limited medical resources.
  • mycobacterial diseases can cause overwhelming, disseminated disease in immunocompromised patients.
  • the eradication of mycobacterial diseases has never been achieved, nor is eradication imminent.
  • tuberculosis TB
  • Tuberculosis is the cause of the largest number of human deaths attributable to a single etiologic agent (see Dye et al., J. Am. Med. Association, 282, 677-686, (1999); and 2000 WHO/OMS Press Release).
  • Mycobacteria other than M tuberculosis are increasingly found in opportunistic infections that plague the AIDS patient.
  • Enormous numbers of MAC are found (up to 10 10 acid-fast bacilli per gram of tissue), and consequently, the prognosis for the infected AIDS patient is poor.
  • the World Health Organization continues to encourage the battle against TB, recommending prevention initiatives such as the “Expanded Program on Immunization” (EPI), and therapeutic compliance initiatives such as “Directly Observed Treatment Short-Course” (DOTS).
  • EPI Program on Immunization
  • DAS Directly Observed Treatment Short-Course
  • diagnosis, treatment, and prevention are equally important. Rapid detection of active TB patients will lead to early treatment by which about 90% cure is. expected. Therefore, early diagnosis is critical for the battle against TB.
  • therapeutic compliance will ensure not only elimination of infection, but also reduction in the emergence of drug-resistance strains.
  • M. tuberculosis M. avium intracellulare
  • M. kansasii M. fortuitum
  • M. chelonae M. leprae.
  • the most prevalent mycobacterial disease in humans is tuberculosis (TB) which is predominantly caused by mycobacterial species comprising M. tuberculosis, M. bovis, or M. africanum (Merck Manual 1992).
  • Infection is typically initiated by the inhalation of infectious particles which are able to reach the terminal pathways in lungs.
  • the bacilli Following engulfment by alveolar macrophages, the bacilli are able to replicate freely, with eventual destruction of the phagocytic cells. A cascade effect ensues wherein destruction of the phagocytic cells causes additional macrophages and lymphocytes to migrate to the site of infection, where they too are ultimately eliminated. The disease is further disseminated during the initial stages by the infected macrophages which travel to local lymph nodes, as well as into the blood stream and other tissues such as the bone marrow, spleen, kidneys, bone and central nervous system. (See Murray et al. Medical Microbiology, The C.V. Mosby Company 219-230 (1990)).
  • M. avium bacilli occur in several distinct colony forms. Bacilli which grow as transparent, or rough, colonies on conventional laboratory media are multiplicable within macrophages in tissue culture, are virulent when injected into susceptible mice, and arc resistant to antibiotics. Rough or transparent bacilli, which are maintained on laboratory culture media, often spontaneously assume an opaque R colony morphology, at which time they are not multiplicable in macrophages, are avirulent in mice, and are highly susceptible to antibiotics. The differences in colony morphology between the transparent, rough and opaque strains of M.
  • avium are almost certainly due to the presence of a glycolipid coating on the surface of transparent and rough organisms which acts as a protective capsule.
  • This capsule, or coating is composed primarily of C-mycosides which apparently shield the virulent M. avium organisms from lysosomal enzymes and antibiotics.
  • the non-virulent opaque forms of M avium have very little C-mycoside on their surface. Both the resistance to antibiotics and the resistance to killing by macrophages have been attributed to the glycolipid barrier on the surface of M. avium.
  • PCR polymerase chain reaction
  • MTD amplified mycobacterium tuberculosis direct, test
  • tuberculin skin test One diagnostic test that is widely used for detecting infections caused by M. tuberculosis is the tuberculin skin test.
  • PPD purified protein derivative
  • the antigen preparation is either injected into the skin intradermally, or is topically applied and is then invasively transported into the skin with the use of a multiprong inoculator (Tine test).
  • Tine test multiprong inoculator
  • Several problems exist with the skin test diagnosis method For example, the Tine test is not generally recommended because the amount of antigen injected into the intradermal layer cannot be accurately controlled. (See Murray et al. Medical Microbiology, The C.V. Mosby Company 219-230 (1990)).
  • tuberculin skin tests are widely used, they typically require two to three days to generate results, and many times, the results are inaccurate since false positives are sometimes seen in subjects who have been exposed to mycobacteria, but are healthy.
  • instances of mis-diagnosis are frequent since a positive result is observed not only in active TB patients, but also in persons vaccinated with Bacille Calmette-Guerin (BCG), and those who had been infected with mycobacteria, but have not developed the disease. It is hard therefore, to distinguish active TB patients from the others, such as household TB contacts, by the tuberculin skin test.
  • BCG Bacille Calmette-Guerin
  • tuberculin test often produces a cross-reaction in those individuals who were infected with mycobacteria other than M tuberculosis (MOTT). Therefore, diagnosis using the skin tests currently available is frequently subject to error and inaccuracies.
  • the standard treatment for tuberculosis caused by drug-sensitive organisms is a six-month regimen consisting of four drugs given for two months, followed by two drugs given for four months.
  • the two most important drugs, given throughout the six-month course of therapy, are isoniazid and rifampin.
  • the regimen is relatively simple, its administration is quite complicated. Daily ingestion of eight or nine pills is often required during the first phase of therapy; a daunting and confusing prospect. Even severely ill patients are often symptom free within a few weeks, and nearly all appear to be cured within a few months. If the treatment is not continued to completion, however, the patient may experience a relapse, and the relapse rate for patients who do not continue treatment to completion is high.
  • Ethambutol is a widely used antibiotic for the treatment of TB, with over 300 million doses delivered for tuberculosis therapy in 1988.
  • Ethambutol developed by Lederle Laboratories in the 1950s, has low toxicity and is a good pharmacokinetic. However, ethambutol has a relatively high Minimum Inhibition Concentration (MIC) of about 5 ⁇ g/ml, and can cause optic neuritis.
  • MIC Minimum Inhibition Concentration
  • the present invention comprises methods and compositions comprising ethylene diamine compounds effective for the treatment of infectious disease.
  • the present invention also provides methods and compositions comprising substituted ethylene diamines having improved anti-mycobacterial activity, including substituted ethylene diamines having improved anti-tuberculosis activity.
  • the present invention contemplates substituted ethylene diamines, which can derive from a variety of amine compounds.
  • the substituted ethylene diamines arc based on the following structure.
  • the substituted ethylene diamine compounds described herein are synthesized and screened for activity as follows.
  • a chemical library of substituted ethylene diamines is prepared on a solid polystyrene support using split and pool technologies. This technique allows for the synthesis of a diverse set of substituted ethylene diamines.
  • These diamines are screened for anti-TB activity using in vitro, biological assays, including a High-Throughput Screening (HTS) assay, based on the recently completed genomic sequence of M tuberculosis, and a Minimum Inhibition Concentration (MIC) assay.
  • HTS High-Throughput Screening
  • MIC Minimum Inhibition Concentration
  • compositions described herein comprise substituted ethylene diamines that are effective against disease caused by infectious organisms, including, but not limited to, bacteria and viruses.
  • One embodiment of the invention provides methods and compositions comprising substituted ethylene diamines that are effective against mycobacterial disease.
  • Another embodiment of the invention provides methods and compositions comprising substituted ethylene diamines that have MIC of 50 ⁇ M or lower for mycobacterial disease.
  • Another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 25 ⁇ M or lower for mycobacterial disease. Yet another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 12.5 ⁇ M or lower for mycobacterial disease. Another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 5 ⁇ M or lower for mycobacterial disease. In another embodiment of the present invention, the methods and compositions comprise substituted ethylene diamines with HTS Luc activity of 10% or greater.
  • the methods and compositions comprise substituted ethylene diamines, wherein one amine group is derived from a primary amine, and wherein the other amine group is derived from a primary or secondary amine.
  • the present invention contemplates various salt complexes and other substituted derivatives of the substituted ethylene diamines.
  • the present invention also contemplates enantiomers and other stereoisomers of the substituted ethylene diamines and their substituted derivatives.
  • the present invention further contemplates treatment for animals, including, but not limited to, humans.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of mycobacterial disease, including but not limited to, tuberculosis.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of infectious diseases using compositions comprising substituted ethylene diamines.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of mycobacterial disease using compositions comprising substituted ethylene diamines.
  • Still another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 50 ⁇ M, or less.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 25 ⁇ M, or less.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 12.5 ⁇ M, or less.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 5 ⁇ M, or less.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has HTS/Luc activity of 10% or greater.
  • Yet another object of the present invention is to provide composition for the therapeutic formulation for the treatment and prevention of mycobacterial disease.
  • Another object of the present invention is to provide compositions for therapeutic formulations for the treatment and prevention of mycobacterial disease caused by mycobacterial species comprising M. tuberculosis complex, M. avium intracellulare, M. kansarii, M. fortuitum, M. chelonoe, M. leprae, M. africanum, M. microti, M. avium paratuberculosis, or M. bovis.
  • FIG. 1 provides representative examples of diamine products synthesized from amino alcohol pre-loaded resins.
  • FIG. 2 provides commercially available amino alcohol pre-loaded resins.
  • FIG. 3 provides Table 1 which shows the prepared library of targeted 21,120 ethambutol analogs.
  • FIG. 4 provides Scheme I, a schematic showing the Synthesis of the original 100,000 compound library of Ethambutol analogs.
  • FIG. 5 provides Scheme 2, a schematic showing the use of amino alcohol pre-loaded resins and amino acids as linker.
  • FIG. 6 provides Scheme 3, a schematic showing further modification of the linker: working with amino alcohol pre-loaded resins.
  • FIG. 7 provides Table 2 which lists the Amino acids used in the library preparation.
  • FIG. 8 provides representative carbonyl compounds used as reagents in the syntheses.
  • FIGS. 9 a - 9 c provides representative examples of MIC and Lux data.
  • FIGS. 10 a and 10 b show the occurrence of alkylating monomers and their chemical structures in the active molecules.
  • FIG. 11 provides a list of hit compounds and their structures.
  • FIG. 12 provides Table 3 which shows the layout for deconvolutions.
  • Tuberculosis is the cause of the largest number of human deaths attributed to a single etiologic agent with two to three million people infected with tuberculosis dying each year. Areas where humans are crowded together, or living in substandard housing, are increasingly found to have persons affected with mycobacteria. Individuals who arc immunocompromised are at great risk of being infected with mycobacteria and dying from such infection. In addition, the emergence of drug-resistant Strains of mycobacteria has led to treatment problems of such infected persons.
  • Chemotherapy is a standard treatment for tuberculosis. Some current chemotherapy treatments require the use of three or four drugs, in combination, administered daily for two months, or administered biweekly for four to twelve months. Table 1 lists several treatment schedules for standard tuberculosis drug regimens.
  • the present invention provides methods and compositions comprising a class of substituted ethylene diamine compounds effective in treatment and prevention of disease caused by microorganisms including, but not limited to, bacteria.
  • the methods and compositions of the present invention are effective in inhibiting the growth of the microorganism, M. tuberculosis.
  • the methods and compositions of the present invention are intended for the treatment of mycobacteria infections in human, as well as other animals.
  • the present invention may be particularly useful for the treatment of cows infected by M. bovis.
  • the term “tuberculosis” comprises disease states usually associated with infections caused by mycobacteria species comprising M. tuberculosis complex.
  • the term “tuberculosis” is also associated with mycobacterial infections caused by mycobacteria other than M. tuberculosis (MOTT).
  • MOTT mycobacterial species include M. avium - intracellulare, M. kansarii, M. fortuitum, M. chelonae, M. leprae, M. africanum, and M. microti, M. avium paratuberculosis, M. intracellulare, M. scrofulaceum, M. xenopi, M. marinum, M. ulcerans.
  • the present invention further comprises methods and compositions effective for the treatment of infectious disease, including but not limited to those caused by bacterial, mycological, parasitic, and viral agents.
  • infectious agents include the following: staphylococcus, streptococcaceae, neisseriaaceae, cocci, enterobacteriaceae, pseudomonadaceae, vibrionaceae, campylobacter, pasteurellaceae, bordetella, francisella, brucella, legionellaceae, bacteroidaceae, gram-negative bacilli, clostridium, corynebacterium, propionibacterium, gram-positive bacilli, anthrax, actinomyces, nocardia, mycobacterium, treponema, borrelia, leptospira, mycoplasma, ureaplasma, rickettsia, chlamydiae, systemic mycoses, opportunistic my
  • the present invention further provides methods and compositions useful for the treatment of infectious disease, including by not limited to, tuberculosis, leprosy, Crohn's Disease, acquired immunodeficiency syndrome, Lyme disease, cat-scratch disease, Rocky Mountain Spotted Fever and influenza.
  • the present invention is particularly directed to a novel library of diamine compounds of Ethambutol family comprising a modified ethylene linker starting from commercially available amino alcohol pre-loaded resins.
  • the compounds in the library were prepared on mmol scale in 96-well format in pools of 10 compounds per well (for the vast majority of the plates).
  • Table 1 ( FIG. 3 ) summarizes data for the synthesized plates.
  • Deprotection (removal of the FMOC group) was carried out by reaction with piperidine at RT. Derivatization of the amino group was achieved by reductive alkylation with 96 various carbonyl compounds, such as aldehydes, ketones, and carboxylic acids, in the presence of NaBCNH 3 at RT for 72-96 h. The selection of the carbonyl compounds was made so, that the final diamine products would carry the same or similar types of substituents that had been observed in the hit compounds generated from the previous library of ethambutol analogs, as well as structural diversity ( FIG. 8 ). A complete list of the carbonyl compounds used is shown in Table 3 ( FIG. 8 ).
  • Reduction of the aminoethyleneamides into corresponding diamines was carried out using the soluble reducing reagent 65+w % Red-Al at room temperature. Cleavage of the products from the resin was achieved with a 10% solution of trifluoroacetic acid in dichloromethane resulting in the formation of TFA salts of the diamines.
  • acylation step of the synthetic scheme was carried out using a Quest 210 Synthesizer on scale of 0.1-0.15 g of resin per tube. Following the reaction, formed resins were thoroughly washed, dried, and then groups of ten resins were pooled together. A small amount of each resin ( ⁇ 0.05 g) was archived prior to pooling to facilitate re-synthesis and deconvolution of actives.
  • the very first step was derivatization of the amino group via reductive alkylation by 10 carbonyl compounds (cyclooctanone, 4-benzyloxybenzaldehyde, (S)-citronellal, myrtenal, tetrahydro-4H-pyran-4-one, norcamphor, 4-(4-hydroxyphenyl)2-butanone, geranylacetone, 2-decalone, 2-adamantanone) in presence of NaBCNH 3 at RT.
  • the following steps were carried out in a similar fashion as we reported earlier for the Scheme 2.
  • compositions containing the substituted ethylene diamine compounds of the present invention can be prepared in physiologically acceptable formulations, such as in pharmaceutically acceptable carriers, using known techniques.
  • a substituted ethylene diamine compound is combined with a pharmaceutically acceptable excipient to form a therapeutic composition.
  • compositions of the present invention may be administered in the form of a solid, liquid or aerosol.
  • solid compositions include pills, creams, soaps and implantable dosage units. Pills may be administered orally.
  • Therapeutic creams and anti-mycobacteria soaps may be administered topically.
  • Implantable dosage units may be administered locally, for example, in the lungs, or may be implanted for systematic release of the therapeutic compositions, for example, subcutaneously.
  • liquid compositions include formulations adapted for injection intramuscularly, subcutaneously, intravenously, intraarterially, and formulations for topical and intraocular administration.
  • aerosol formulations include inhaler formulations for administration to the lungs.
  • a sustained release matrix is a matrix made of materials, usually polymers, which are degradable by enzymatic or acid/base hydrolysis, or by dissolution. Once inserted into the body, the matrix is acted upon by enzymes and body fluids.
  • the sustained release matrix is chosen desirably from biocompatible materials, including, but not limited to, liposomes, polylactides, polyglycolide (polymer of glycolic acid), polylactide co-glycolide (copolymers of lactic acid and glycolic acid), polyanhydrides, poly(ortho)esters, polypeptides, hyaluronic acid, collagen, chondroitin sulfate, carboxylic acids, fatty acids, phospholipds, polysaccharides, nucleic acids, polyamino acids, amino acids such as phenylalanine, tyrosine, isoleucine, polynucleotides, polyvinyl propylene, polyvinylpyrrolidone and silicone.
  • a preferred biodegradable matrix is a matrix of one of either polylactide, polyglycolide, or polylactide co-glycolide.
  • the dosage of the composition will depend on the condition being treated, the particular composition used, and other clinical factors, such as weight and condition of the patient, and the route of administration.
  • a suitable dosage may range from 100 to 0.1 mg/kg.
  • a more preferred dosage may range from 50 to 0.2 mg/kg.
  • a more preferred dosage may range from 25 to 0.5 mg/kg.
  • Tablets or other forms of media may contain from 1 to 1000 mg of the substituted ethylene diamine. Dosage ranges and schedules of administration similar to ethambutol or other anti-tuberculosis drugs may be used.
  • compositions may be administered in combination with other compositions and procedures for the treatment of other disorders occurring in combination with mycobacterial disease.
  • tuberculosis frequently occurs as a secondary complication associated with -acquired immunodeficiency syndrome (AIDS).
  • AIDS immunodeficiency syndrome
  • Patients undergoing AIDS treatment which includes procedures such as surgery, radiation or chemotherapy, may benefit from the therapeutic methods and compositions described herein.
  • the acylation step was carried out in 5 ml tubes using the Quest 210 Synthesizer. Removal of the FMOC group, reductive alkylation reaction with carbonyl compounds, the reduction with Red-Al, and the cleavage from the solid support were carried out in 96-deep (2 ml) well, chemically resistant plates.
  • Step 1 Acylation of Amino Alcohol Pre-Loaded Resins with Amino Acids.
  • each tube was loaded 0.150 g of corresponding resin (coverage of 0.3-1.0 mmol/g), and all resins were pre-swollen in DCM for 1.5 h and filtered. If Fmoc protected resin was used: the resin was stirred with 2.5 ml of 20% solution of piperidine in DMF for 10 min, filtered, and washed with 2.5 ml of DMF. The procedure was repeated, but the stirring time was 20 min. After that all resins were filtered, washed with DMF (1 ⁇ 2.5 ml) and DCM (2 ⁇ 3 ml). Each tube was charged with 1 ml of dichloromethane.
  • the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1 ⁇ 3 ml), dichloromethane (1 ⁇ 3 ml), and acylation was repeated with the same amount of reagents.
  • the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1 ⁇ 3 ml), methanol (3 ⁇ 3 ml), sucked dry (on Quest) for 30 min and transferred into vials (one resin per vial), and dried in a desiccator under vacuum for 1 h. After this step all resins were subjected for quality control using MS spectra.
  • Each well in row A on the reaction plate was charged with 0.1 ml of dichloromethane, 0.08 ml of ⁇ 1.0M solution of appropriate acid in DMF from master plate, 0.05 ml DMF solution of PyBrop, (0.012 g, 0.025 mmol, 2.5 mol excess to loaded resin) and 0.05 ml of EtNiPr 2 in dichloromethane (0.017 ml, 0.10 mmol, 10 mol excess to loaded resin).
  • Each well in rows B through H was charged with 0.1 ml of THF, 0.160 ml of ⁇ 1.0 M solution of appropriate aldehyde or ketone in DMF from master plate and allowed to react for 30 min.
  • reaction plates were placed into combiclamps.
  • a 1:6 mixture of Red-Al (65+w % in toluene) and THF was added, 0.6 ml per well (0.28 mmol of Red-Al per well), and allowed to react for 4 h.
  • the resins were filtered, washed with THF (2 ⁇ 1 ml), methanol (3 ⁇ 1 ml) and dried in the filtration manifold.
  • This step was carried out using a cleavage manifold.
  • the reaction plates placed on the top of the collection plates in this manifold) were charged with a 10:88:2 mixture of TFA, dichloromethane, and triisopropylsilane, 0.5 ml per well. After 15 min, the solutions were filtered and collected into proper wells of the collection plates. The procedure was repeated. Solvents were evaporated on a speedvac, and the residual samples were ready for testing.
  • the reductive alkylation step of 4-aminobutan-1 of resin and the acylation step were carried out in 5 ml tubes using the Quest 210 Synthesizer. Removal of the FMOC group, reductive alkylation reaction with carbonyl compounds, the reduction with Red-Al, and the cleavage from the solid support were carried out in 96-deep (2 ml) well, chemically resistant plates.
  • Step 1 Reductive Alkylation of 4-Aminobutan-1-ol resin.
  • a suspension of the resin (coverage of 0.3-1.0 mmol/g), 1.0 g (up to 1.0 mmol), in 30 ml of 2:1 mixture of dichloromethane and THF was distribution into 10 tubes, 3 ml per tube and filtered, than each tube was charged with 0.10 g of resin. Resin was pre-swollen in DCM for 1.5 h and filtered. Each tube were loaded with 1.5 ml 1,2-dichloroethane, 0.3 mmol (3 mol excess) of corresponded aldehyde or ketone (alkylating reagent) and allowed to react for 30 min.
  • Deconvolution of the active wells was performed by re-synthesis of discrete compounds, from the archived FMOC-protected-aminoacetamide resins (10 resins, 0.05-0.10 g each), which were set aside at the end of the acylation step before the pooling.
  • Each resin was assigned a discrete column (1, or 2, or 3, etc.) in a 96-well filterplate, and was divided between X rows (A, B, C, etc), where X is the number of hits discovered in the original screening plate.
  • a selected carbonyl compound present in the hit was added along with other required reagents:
  • the first selected carbonyl compound was added to the resins in the row “A”, the second carbonyl compound—to the resins in the row “B”, the third carbonyl compound—to the resins in the row “C”, etc.
  • a lay-out of a representative 96-well deconvolution plate is shown in Table 3, FIG. 12 .
  • reaction plates were sealed and kept at RT for 72 h. At the end, the resins were filtered, washed with THF, DCM (1 ⁇ 1 ml), methanol (2 ⁇ 1 ml) and dried in desiccator under vacuum for 2 h. Reduction and cleavage were performed according to steps 5 and 6 of the synthetic protocol. The product wells from the cleavage were analyzed by ESI-MS (Electrospray Ionization Mass Spectroscopy) to ensure the identity of the actives, and were tested in the MIC assay.
  • ESI-MS Electrononization Mass Spectroscopy
  • the resin was filtered, washed with 1:1 mixture of DMF and dichloromethane (1 ⁇ 6 ml), dichloromethane (1 ⁇ 6 ml), and acylation was repeated with the same amount of reagents. At the end, the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (2 ⁇ 6 ml).

Abstract

Methods and compositions for treating disease caused by infectious agents, particularly tuberculosis. In particular, methods and compositions comprising substituted diamines for the treatment of infectious diseases are provided. In one embodiment, these methods and compositions are used for the treatment of mycobacterial infections, including, but not limited to, tuberculosis.

Description

    CROSS REFERENCE TO RELATED PATENT APPLICATIONS
  • The present application claims priority to U.S. Provisional Patent Application Ser. No. 60/381,244 filed May 17, 2002 and is a Continuation appkation of U.S. patent application Ser. No. 10/441,272 filed May 19, 2003 now U.S. Pat. No. 7,652,039 issued Jan. 26, 2010. The present application is related to pending U.S. patent application Ser. No. 10/147,587 filed May 17, 2002.
  • FIELD OF INVENTION
  • The present invention relates to methods and compositions for treating disease caused by microorganisms, particularly tuberculosis. The present invention also relates to methods and compositions having improved anti-mycobacterial activity, namely compositions comprising novel substituted ethylene diamine compounds.
  • BACKGROUND OF THE INVENTION
  • Mycobacterial infections often manifest as diseases such as tuberculosis. Human infections caused by mycobacteria have been widespread since ancient times, and tuberculosis remains a leading cause of death today. Although the incidence of the disease declined, in parallel with advancing standards of living, since the mid-nineteenth century, mycobacterial diseases still constitute a leading cause of morbidity and mortality in countries with limited medical resources. Additionally, mycobacterial diseases can cause overwhelming, disseminated disease in immunocompromised patients. In spite of the efforts of numerous health organizations worldwide, the eradication of mycobacterial diseases has never been achieved, nor is eradication imminent. Nearly one third of the world's population is infected with mycobacterium tuberculosis complex, commonly referred to as tuberculosis (TB), with approximately 8 million new cases, and two to three million deaths attributable to TB yearly. Tuberculosis (TB) is the cause of the largest number of human deaths attributable to a single etiologic agent (see Dye et al., J. Am. Med. Association, 282, 677-686, (1999); and 2000 WHO/OMS Press Release).
  • After decades of decline, TB is now on the rise. In the United States, up to 10 million individuals are believed to be infected. Almost 28,000 new cases were reported in 1990, constituting a 9.4 percent increase over 1989. A sixteen percent increase in TB cases was observed from 1985 to 1990. Overcrowded living conditions and shared air spaces are especially conducive to the spread of TB, contributing to the increase in instances that have been observed among prison inmates, and among the homeless in larger U.S. cities. Approximately half of all patients with “Acquired Immune Deficiency Syndrome” (AIDS) will acquire a mycobacterial infection, with TB being an especially devastating complication. AIDS patients are at higher risks of developing clinical TB, and anti-TB treatment seems to be less effective than in non-AIDS patients. Consequently, the infection often progresses to a fatal disseminated disease.
  • Mycobacteria other than M tuberculosis are increasingly found in opportunistic infections that plague the AIDS patient. Organisms from the M. avium-intracellulare complex (MAC), especially serotypes four and eight, account for 68% of the mycobacterial isolates from AIDS patients. Enormous numbers of MAC are found (up to 1010 acid-fast bacilli per gram of tissue), and consequently, the prognosis for the infected AIDS patient is poor.
  • The World Health Organization (WHO) continues to encourage the battle against TB, recommending prevention initiatives such as the “Expanded Program on Immunization” (EPI), and therapeutic compliance initiatives such as “Directly Observed Treatment Short-Course” (DOTS). For the eradication of TB, diagnosis, treatment, and prevention are equally important. Rapid detection of active TB patients will lead to early treatment by which about 90% cure is. expected. Therefore, early diagnosis is critical for the battle against TB. In addition, therapeutic compliance will ensure not only elimination of infection, but also reduction in the emergence of drug-resistance strains.
  • The emergence of drug-resistant M. tuberculosis is an extremely disturbing phenomenon. The rate of new TB cases proven resistant to at least one standard drug increased from 10 percent in the early 1980′s to 23 percent in 1991. Compliance with therapeutic regimens, therefore, is also a crucial component in efforts to eliminate TB and prevent the emergence of drug resistant strains. Equally important is the development of new therapeutic agents that are effective as vaccines, and as treatments, for disease caused by drug resistant strains of mycobacteria.
  • Although over 37 species of mycobacteria have been identified, more than 95% of all human infections are caused by six species of mycobacteria: M. tuberculosis, M. avium intracellulare, M. kansasii, M. fortuitum, M. chelonae, and M. leprae. The most prevalent mycobacterial disease in humans is tuberculosis (TB) which is predominantly caused by mycobacterial species comprising M. tuberculosis, M. bovis, or M. africanum (Merck Manual 1992). Infection is typically initiated by the inhalation of infectious particles which are able to reach the terminal pathways in lungs. Following engulfment by alveolar macrophages, the bacilli are able to replicate freely, with eventual destruction of the phagocytic cells. A cascade effect ensues wherein destruction of the phagocytic cells causes additional macrophages and lymphocytes to migrate to the site of infection, where they too are ultimately eliminated. The disease is further disseminated during the initial stages by the infected macrophages which travel to local lymph nodes, as well as into the blood stream and other tissues such as the bone marrow, spleen, kidneys, bone and central nervous system. (See Murray et al. Medical Microbiology, The C.V. Mosby Company 219-230 (1990)).
  • There is still no clear understanding of the factors which contribute to the virulence of mycobacteria. Many investigators have implicated lipids of the cell wall and bacterial surface as contributors to colony morphology and virulence. Evidence suggests that C-mycosides, on the surface of certain mycobacterial cells, are important in facilitating survival of the organism within macrophages. Trehalose 6,6′ dimycolate, a cord factor, bas been implicated for other mycobacteria.
  • The interrelationship of colony morphology and virulence is particularly pronounced in M. avium. M. avium bacilli occur in several distinct colony forms. Bacilli which grow as transparent, or rough, colonies on conventional laboratory media are multiplicable within macrophages in tissue culture, are virulent when injected into susceptible mice, and arc resistant to antibiotics. Rough or transparent bacilli, which are maintained on laboratory culture media, often spontaneously assume an opaque R colony morphology, at which time they are not multiplicable in macrophages, are avirulent in mice, and are highly susceptible to antibiotics. The differences in colony morphology between the transparent, rough and opaque strains of M. avium are almost certainly due to the presence of a glycolipid coating on the surface of transparent and rough organisms which acts as a protective capsule. This capsule, or coating, is composed primarily of C-mycosides which apparently shield the virulent M. avium organisms from lysosomal enzymes and antibiotics. By contrast, the non-virulent opaque forms of M avium have very little C-mycoside on their surface. Both the resistance to antibiotics and the resistance to killing by macrophages have been attributed to the glycolipid barrier on the surface of M. avium.
  • Diagnosis of mycobacterial infection is confirmed by the isolation and identification of the pathogen, although conventional diagnosis is based on sputum smears, chest X-ray examination (CXR), and clinical symptoms. Isolation of mycobacteria on a medium takes as long as four to eight weeks. Species identification takes a further two weeks. There are several other techniques for detecting mycobacteria such as the polymerase chain reaction (PCR), mycobacterium tuberculosis direct test, or amplified mycobacterium tuberculosis direct, test (MTD), and detection assays that utilize radioactive labels.
  • One diagnostic test that is widely used for detecting infections caused by M. tuberculosis is the tuberculin skin test. Although numerous versions of the skin test are available, typically one of two preparations of tuberculin antigens are used: old tuberculin (OT); or purified protein derivative (PPD). The antigen preparation is either injected into the skin intradermally, or is topically applied and is then invasively transported into the skin with the use of a multiprong inoculator (Tine test). Several problems exist with the skin test diagnosis method. For example, the Tine test is not generally recommended because the amount of antigen injected into the intradermal layer cannot be accurately controlled. (See Murray et al. Medical Microbiology, The C.V. Mosby Company 219-230 (1990)).
  • Although the tuberculin skin tests are widely used, they typically require two to three days to generate results, and many times, the results are inaccurate since false positives are sometimes seen in subjects who have been exposed to mycobacteria, but are healthy. In addition, instances of mis-diagnosis are frequent since a positive result is observed not only in active TB patients, but also in persons vaccinated with Bacille Calmette-Guerin (BCG), and those who had been infected with mycobacteria, but have not developed the disease. It is hard therefore, to distinguish active TB patients from the others, such as household TB contacts, by the tuberculin skin test. Additionally, the tuberculin test often produces a cross-reaction in those individuals who were infected with mycobacteria other than M tuberculosis (MOTT). Therefore, diagnosis using the skin tests currently available is frequently subject to error and inaccuracies.
  • The standard treatment for tuberculosis caused by drug-sensitive organisms is a six-month regimen consisting of four drugs given for two months, followed by two drugs given for four months. The two most important drugs, given throughout the six-month course of therapy, are isoniazid and rifampin. Although the regimen is relatively simple, its administration is quite complicated. Daily ingestion of eight or nine pills is often required during the first phase of therapy; a daunting and confusing prospect. Even severely ill patients are often symptom free within a few weeks, and nearly all appear to be cured within a few months. If the treatment is not continued to completion, however, the patient may experience a relapse, and the relapse rate for patients who do not continue treatment to completion is high. A variety of forms of patient-centered care are used to promote adherence with therapy. The most effective way of ensuring that patients are taking their medication is to use directly observed therapy, which involves having a member of the health care team observe the patient take each dose of each drug. Directly observed therapy can be provided in the clinic, the patient's residence, or any mutually agreed upon site. Nearly all patients who have tuberculosis caused by drug-sensitive organisms, and who complete therapy will be cured, and the risk of relapse is very low (“Ending Neglect: The Elimination of Tuberculosis in the United States” ed. L. Geiter Committee on the Elimination of Tuberculosis in the United States Division of Health Promotion and Disease Prevention, Institute of Medicine. Unpublished.)
  • What is needed are effective therapeutic regimens that include improved vaccination and treatment protocols. Currently available therapeutics are no longer consistently effective as a result of the problems with treatment compliance, and these compliance problems contribute to the development of drug resistant mycobacterial strains.
  • Ethambutol (EMB) is a widely used antibiotic for the treatment of TB, with over 300 million doses delivered for tuberculosis therapy in 1988.
  • Figure US20100273826A1-20101028-C00001
  • Ethambutol, developed by Lederle Laboratories in the 1950s, has low toxicity and is a good pharmacokinetic. However, ethambutol has a relatively high Minimum Inhibition Concentration (MIC) of about 5 μg/ml, and can cause optic neuritis. Thus, there is an increasing need for new, and more effective, therapeutic compositions (See for example, U.S. Pat. No. 3,176,040, U.S. Pat. No. 4,262,122; U.S. Pat. No. 4,006,234; U.S. Pat. No. 3,931,157; U.S. Pat. No. 3,931,152; U.S. Re. 29,358; and Hãusler et al., Bioorganic & Medicinal Chemistry Letters 11 (2001) 1679-1681). In the decoder years since the discovery of the beneficial effects of ethambutol, few pharmacological advances in TB treatment have been developed. Moreover, with the combined emergence of drug resistant strains, and the more prevalent spread of mycobacterial disease, it is becoming seriously apparent that new therapeutic compositions are crucial in the fight against tuberculosis.
  • Clearly effective therapeutic regimens that include improved vaccination and treatment protocols arc needed. A therapeutic vaccine that would prevent the onset of tuberculosis, and therefore eliminate the need for therapy is desirable. Although currently available therapeutics such as ethambutol are effective, the emergence of drug resistant strains has necessitated new formulations and compositions that are more versatile than ethambutol. Currently available therapeutics are no longer consistently effective as a result of the problems with treatment compliance, lending to the development of drug resistant mycobacterial strains. What is needed are new anti-tubercular drugs that provide highly effective treatment, and shorten or simplify tuberculosis chemotherapy.
  • SUMMARY OF THE INVENTION
  • The present invention comprises methods and compositions comprising ethylene diamine compounds effective for the treatment of infectious disease. The present invention also provides methods and compositions comprising substituted ethylene diamines having improved anti-mycobacterial activity, including substituted ethylene diamines having improved anti-tuberculosis activity.
  • The present invention contemplates substituted ethylene diamines, which can derive from a variety of amine compounds. In the present invention, the substituted ethylene diamines arc based on the following structure.
  • Figure US20100273826A1-20101028-C00002
  • The substituted ethylene diamine compounds described herein are synthesized and screened for activity as follows. A chemical library of substituted ethylene diamines is prepared on a solid polystyrene support using split and pool technologies. This technique allows for the synthesis of a diverse set of substituted ethylene diamines. These diamines are screened for anti-TB activity using in vitro, biological assays, including a High-Throughput Screening (HTS) assay, based on the recently completed genomic sequence of M tuberculosis, and a Minimum Inhibition Concentration (MIC) assay.
  • The methods and compositions described herein comprise substituted ethylene diamines that are effective against disease caused by infectious organisms, including, but not limited to, bacteria and viruses.
  • One embodiment of the invention provides methods and compositions comprising substituted ethylene diamines that are effective against mycobacterial disease.
  • Another embodiment of the invention provides methods and compositions comprising substituted ethylene diamines that have MIC of 50 μM or lower for mycobacterial disease.
  • Another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 25 μM or lower for mycobacterial disease. Yet another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 12.5 μM or lower for mycobacterial disease. Another embodiment of the present invention comprises substituted ethylene diamines that have an MIC of 5 μM or lower for mycobacterial disease. In another embodiment of the present invention, the methods and compositions comprise substituted ethylene diamines with HTS Luc activity of 10% or greater.
  • In yet another embodiment of the present invention, the methods and compositions comprise substituted ethylene diamines, wherein one amine group is derived from a primary amine, and wherein the other amine group is derived from a primary or secondary amine.
  • The present invention contemplates various salt complexes and other substituted derivatives of the substituted ethylene diamines. The present invention also contemplates enantiomers and other stereoisomers of the substituted ethylene diamines and their substituted derivatives. The present invention further contemplates treatment for animals, including, but not limited to, humans.
  • Accordingly, it is an object of the present invention to provide methods and compositions for the treatment and prevention of diseases caused by infectious agents.
  • Accordingly, it is an object of the present invention to provide methods and compositions for the treatment and prevention of infectious diseases.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of mycobacterial disease, including but not limited to, tuberculosis.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of infectious diseases using compositions comprising substituted ethylene diamines.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of mycobacterial disease using compositions comprising substituted ethylene diamines.
  • Still another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 50 μM, or less.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 25 μM, or less.
  • Another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 12.5 μM, or less.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has an MIC of 5 μM, or less.
  • Yet another object of the present invention is to provide methods and compositions for the treatment and prevention of tuberculosis using compositions comprising substituted ethylene diamines, wherein the diamine has HTS/Luc activity of 10% or greater.
  • Yet another object of the present invention is to provide composition for the therapeutic formulation for the treatment and prevention of mycobacterial disease.
  • Another object of the present invention is to provide compositions for therapeutic formulations for the treatment and prevention of mycobacterial disease caused by mycobacterial species comprising M. tuberculosis complex, M. avium intracellulare, M. kansarii, M. fortuitum, M. chelonoe, M. leprae, M. africanum, M. microti, M. avium paratuberculosis, or M. bovis.
  • These and other objects, features and advantages of the present invention will become apparent after a review of the following detailed description of the disclosed embodiments and the appended claims.
  • BRIEF DESCRIPTION OF THE FIGURES
  • FIG. 1 provides representative examples of diamine products synthesized from amino alcohol pre-loaded resins.
  • FIG. 2 provides commercially available amino alcohol pre-loaded resins.
  • FIG. 3 provides Table 1 which shows the prepared library of targeted 21,120 ethambutol analogs.
  • FIG. 4 provides Scheme I, a schematic showing the Synthesis of the original 100,000 compound library of Ethambutol analogs.
  • FIG. 5 provides Scheme 2, a schematic showing the use of amino alcohol pre-loaded resins and amino acids as linker.
  • FIG. 6 provides Scheme 3, a schematic showing further modification of the linker: working with amino alcohol pre-loaded resins.
  • FIG. 7 provides Table 2 which lists the Amino acids used in the library preparation.
  • FIG. 8 provides representative carbonyl compounds used as reagents in the syntheses.
  • FIGS. 9 a-9 c provides representative examples of MIC and Lux data.
  • FIGS. 10 a and 10 b show the occurrence of alkylating monomers and their chemical structures in the active molecules.
  • FIG. 11 provides a list of hit compounds and their structures.
  • FIG. 12 provides Table 3 which shows the layout for deconvolutions.
  • DETAILED DESCRIPTION
  • The present invention may be understood more readily by reference to the following detailed description of the specific embodiments included herein. However, although the present invention has been described with reference to specific details of certain embodiments thereof, it is not intended that such details should be regarded as limitations upon the scope of the invention. The entire text of the references mentioned herein are hereby incorporated in their entireties by reference including United States Provisional Patent Application Ser. No. 60/381,244 filed May 17, 2002 and U.S. patent application Ser. No. 10/147,587 filed May 17, 2002.
  • Mycobacterial infections, such as those causing tuberculosis, once thought to be declining in occurrence, have rebounded, and again constitute a serious health threat. Tuberculosis (TB) is the cause of the largest number of human deaths attributed to a single etiologic agent with two to three million people infected with tuberculosis dying each year. Areas where humans are crowded together, or living in substandard housing, are increasingly found to have persons affected with mycobacteria. Individuals who arc immunocompromised are at great risk of being infected with mycobacteria and dying from such infection. In addition, the emergence of drug-resistant Strains of mycobacteria has led to treatment problems of such infected persons.
  • Many people who are infected with mycobacteria are poor, or live in areas with inadequate healthcare facilities. As a result of various obstacles (economical, education levels, etc.), many of these individuals are unable to comply with the prescribed therapeutic regimens. Ultimately, persistent non-compliance by these and other individuals results in the prevalence of disease. This noncompliance is frequently compounded by the emergence of drug-resistant strains of mycobacteria. Effective compositions and vaccines that target various strains of mycobacteria are necessary to bring the increasing number of tuberculosis cases under control.
  • Chemotherapy is a standard treatment for tuberculosis. Some current chemotherapy treatments require the use of three or four drugs, in combination, administered daily for two months, or administered biweekly for four to twelve months. Table 1 lists several treatment schedules for standard tuberculosis drug regimens.
  • TABLE 1
    Treatment Schedules for Standard TB Drug Regimens.
    STANDARD INDUCTION CONTINUATION
    DRUG PHASE PHASE
    REGIMEN Dosing Schedule DURATION DRUG Dosing Schedule DURATION
    Isoniazid Daily, DOT 8 weeks Isoniazid 2/weeks, DOT 16 weeks
    Rifampicin Daily, DOT 8 weeks Rifampien 2/weeks, DOT 16 weeks
    Pyrazinamide Daily, DOT 8 weeks
    Ethambutol or Daily, DOT 8 weeks
    Streptomycin
  • Decades of misuse of existing antibiotics and poor compliance with prolong and complex therapeutic regimens has led to mutations of the mycobacterium tuberculosis and has created an epidemic of drug resistance that threatens tuberculosis control world wide. The vast majority of currently prescribed drugs, including the front line drugs, such as isoniazid, rifampin, pyrazinamide, ethambutol and streptomycin were developed from the 1950s to the 1970s. Thus, this earlier development of tuberculosis chemotherapy did not have at its disposal the implications of the genome sequence of Mycobacterium tuberculosis, the revolution in pharmaceutical drug discovery of the last decades, and the use of national drug testing and combinational chemistry.
  • Consequently, the treatments of drug-resistant M. tuberculosis strains, and latent tuberculosis infections, require new anti-tuberculosis drugs that provide highly effective treatments, and shortened and simplified tuberculosis chemotherapies. Moreover, it is desirable that these drugs be prepared by a low-cost synthesis, since the demographics of the disease dictate that cost is a significant factor.
  • The present invention provides methods and compositions comprising a class of substituted ethylene diamine compounds effective in treatment and prevention of disease caused by microorganisms including, but not limited to, bacteria. In particular, the methods and compositions of the present invention are effective in inhibiting the growth of the microorganism, M. tuberculosis. The methods and compositions of the present invention are intended for the treatment of mycobacteria infections in human, as well as other animals. For example, the present invention may be particularly useful for the treatment of cows infected by M. bovis.
  • As used herein, the term “tuberculosis” comprises disease states usually associated with infections caused by mycobacteria species comprising M. tuberculosis complex. The term “tuberculosis” is also associated with mycobacterial infections caused by mycobacteria other than M. tuberculosis (MOTT). Other mycobacterial species include M. avium-intracellulare, M. kansarii, M. fortuitum, M. chelonae, M. leprae, M. africanum, and M. microti, M. avium paratuberculosis, M. intracellulare, M. scrofulaceum, M. xenopi, M. marinum, M. ulcerans.
  • The present invention further comprises methods and compositions effective for the treatment of infectious disease, including but not limited to those caused by bacterial, mycological, parasitic, and viral agents. Examples of such infectious agents include the following: staphylococcus, streptococcaceae, neisseriaaceae, cocci, enterobacteriaceae, pseudomonadaceae, vibrionaceae, campylobacter, pasteurellaceae, bordetella, francisella, brucella, legionellaceae, bacteroidaceae, gram-negative bacilli, clostridium, corynebacterium, propionibacterium, gram-positive bacilli, anthrax, actinomyces, nocardia, mycobacterium, treponema, borrelia, leptospira, mycoplasma, ureaplasma, rickettsia, chlamydiae, systemic mycoses, opportunistic mycoses, protozoa, nematodes, trematodes, cestodes, adenoviruses, herpesviruses, poxviruses, papovaviruses, hepatitis viruses, orthomyxoviruses, paramyxoviruses, coronaviruses, picomaviruses, reoviruses, togaviruses, flaviviruses, bunyaviridae, rhabdoviruses, human immunodeficiency virus and retroviruses.
  • The present invention further provides methods and compositions useful for the treatment of infectious disease, including by not limited to, tuberculosis, leprosy, Crohn's Disease, acquired immunodeficiency syndrome, Lyme disease, cat-scratch disease, Rocky Mountain Spotted Fever and influenza.
  • Second Generation Antibiotics from Ethambutol
  • The present invention is particularly directed to a novel library of diamine compounds of Ethambutol family comprising a modified ethylene linker starting from commercially available amino alcohol pre-loaded resins.
  • Figure US20100273826A1-20101028-C00003
  • In an effort to enhance the structural diversity of our library of Ethambutol analogs and to assess the influence of a modified linker on the activity of structurally diverse diamines against M. tuberculosis, the synthetic scheme to incorporate amino acids into the bridging linker between the two amine components was modified. Use of amino acids was of a special interest since it allowed introduction of another diversity element into the linker (as R4), as well as chirality.
  • It was crucial for the project to synthesize a sub-library of diamines closely related to Ethambutol by having amino alcohol moiety in the molecule, and yet quite different due to presence an elaborate linker between the two amine atoms (see FIG. 1 for representative examples). Also, the fact that the 2-Chlorotrityl resins with pre-loaded amino alcohols are commercially available (FIG. 2) and suit well proposed chemistry was very attractive.
  • The compounds in the library were prepared on mmol scale in 96-well format in pools of 10 compounds per well (for the vast majority of the plates). Table 1 (FIG. 3) summarizes data for the synthesized plates.
  • Solid phase syntheses using amino alcohol pre-loaded resins. Twenty 96-well plates have been prepared. Four- and five-steps synthetic routes starting from commercially available amino alcohol pre-loaded resins similar to what had been used to create our first 100,000 compound library (Scheme 1, FIG. 4), were applied to make targeted diamines ( Schemes 2 and 3, FIGS. 5 and 6 respectively). There are some differences in the syntheses: (1) two first steps of the Scheme 1 are abandoned in Schemes 2 and 3; (2) in the Scheme 1, the second amine is introduced into the molecule as a whole synthon via nucleophilic displacement of Cl-function of the linker, while in the Schemes 2 and 3, it proceeds through modification of the existing amino moiety by carbonyl compounds.
  • Scheme 2. Acylation of the purchased amino alcohol pre-loaded 2-Chlorotrityl resins was accomplished via peptide coupling with FMOC protected amino acids in presence of HATU (O-(7-Azabenzotriazol-1-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate) and EtN(iso-Pr)2 in DCM/DMF mixture at RT. The reaction was done twice to improve product yields. The list of the amino acids used to create this library is shown In the Table 2 (FIG. 7).
  • Deprotection (removal of the FMOC group) was carried out by reaction with piperidine at RT. Derivatization of the amino group was achieved by reductive alkylation with 96 various carbonyl compounds, such as aldehydes, ketones, and carboxylic acids, in the presence of NaBCNH3 at RT for 72-96 h. The selection of the carbonyl compounds was made so, that the final diamine products would carry the same or similar types of substituents that had been observed in the hit compounds generated from the previous library of ethambutol analogs, as well as structural diversity (FIG. 8). A complete list of the carbonyl compounds used is shown in Table 3 (FIG. 8).
  • Reduction of the aminoethyleneamides into corresponding diamines was carried out using the soluble reducing reagent 65+w % Red-Al at room temperature. Cleavage of the products from the resin was achieved with a 10% solution of trifluoroacetic acid in dichloromethane resulting in the formation of TFA salts of the diamines.
  • For library production the acylation step of the synthetic scheme was carried out using a Quest 210 Synthesizer on scale of 0.1-0.15 g of resin per tube. Following the reaction, formed resins were thoroughly washed, dried, and then groups of ten resins were pooled together. A small amount of each resin (˜0.05 g) was archived prior to pooling to facilitate re-synthesis and deconvolution of actives.
  • Deprotection of the FMOC group, addition of the carbonyl component, reduction, and cleavage were carried out in 96-well reaction blocks using the Combiclamps system by Whatman Polyfiltronics or the FlexChem system by Robbins Scientific. A suspension of the pooled resins in 2:1 mixture of DCM/THF was evenly distributed into one reaction plate resulting in approximately 10 mg of the resin per well. The 96 diverse carbonyl compounds were arrayed in one 96-well plate template and added, one carbonyl compound per well, to each individual pool of ten resins, resulting in an anticipated 960 diamines produced per plate. Reduction was carried out in the same format and cleavage and filtering into storage plates was followed by evaporation of the TFA prior to biological assay.
  • Quality assessment of the prepared library of diamines was done by Electrospray Ionization mass spectrometry using two randomly selected rows (16 samples) per plate, 17% of the total number. Successful production of a compound was based on an appearance of a molecular ion of the calculated mass. Depending on the amino acid that had been used for the synthesis, the percentage of the predicted ions were observed and therefore the predicted compounds were formed, varied from 31-96% (Table 1, FIG. 3). Based on MS analysis, out of targeted 15,360 compounds, 7,500 diamines were actually formed. Amino acids such as aminomethylcylcohexyl carboxylic acid, thienylalanine, or phenylalanine produced desirable compounds with good yields (88-96%). At the same time, some amino acids, such as arginine, tetrahydroisoquinoline carboxylic acid, and thiazolidine carboxylic acid did not lead to the corresponding products.
  • Scheme 3. Success in preparation of ethambutol analogs with modified linker encouraged us to attempt synthesis of another sub-library of diamines using commercially available amino-alcohol pre-loaded resins (Scheme 3, FIG. 6). This route yields diamine compounds that are similar to those produced by the (Scheme 2, FIG. 5) but that also possess desirable substituents at the first nitrogen atom.
  • We have illustrated this method by starting from commercially available 1,4-aminobutanol pre-loaded resin. We prepared five plates using five amino acids Phe, Amc, Cha, Trp, and Inp (Table 1) that gave the best preliminary results in the screening assays (see number of hits in the Table 1). The very first step was derivatization of the amino group via reductive alkylation by 10 carbonyl compounds (cyclooctanone, 4-benzyloxybenzaldehyde, (S)-citronellal, myrtenal, tetrahydro-4H-pyran-4-one, norcamphor, 4-(4-hydroxyphenyl)2-butanone, geranylacetone, 2-decalone, 2-adamantanone) in presence of NaBCNH3 at RT. The following steps were carried out in a similar fashion as we reported earlier for the Scheme 2.
  • Screening the library against M tuberculosis and deconvolution of the active mixtures. A high-throughput assay with recombinant mycobacteria containing a promoter fusion of luciferase to Rv0341, as well as the MICs, has been used to screen this new compound library of ethambutol analogs, FIG. 9.
  • 198 compound mixtures have shown to exhibit anti-TB activity, Table 1 (activity at <12.5 μM in the HTS Luc assay and/or with an MIC of <12.5 μM) have been selected for deconvolutions. Deconvolutions of all 198 compound mixtures were performed by the discrete re-synthesis of the diamine compounds in 96-well format using stored archive resins (prior pooling them together) and the same synthetic Schemes 2 and 3. The same screening tests were used for every deconvoluted plate. Few carbonyl compounds have been identified as potent synthons contributing to the anti-TB activity (FIG. 5). Performed deconvolutions revealed 118 hits of novel structures as potent anti-TB compounds (Table 4), 38 of those compounds were proven to be active in both assays. FIG. 11 provides a list of hit compounds and their structures.
  • Formulations
  • Therapeutics, including compositions containing the substituted ethylene diamine compounds of the present invention, can be prepared in physiologically acceptable formulations, such as in pharmaceutically acceptable carriers, using known techniques. For example, a substituted ethylene diamine compound is combined with a pharmaceutically acceptable excipient to form a therapeutic composition.
  • The compositions of the present invention may be administered in the form of a solid, liquid or aerosol. Examples of solid compositions include pills, creams, soaps and implantable dosage units. Pills may be administered orally. Therapeutic creams and anti-mycobacteria soaps may be administered topically. Implantable dosage units may be administered locally, for example, in the lungs, or may be implanted for systematic release of the therapeutic compositions, for example, subcutaneously.
  • Examples of liquid compositions include formulations adapted for injection intramuscularly, subcutaneously, intravenously, intraarterially, and formulations for topical and intraocular administration. Examples of aerosol formulations include inhaler formulations for administration to the lungs.
  • A sustained release matrix, as used herein, is a matrix made of materials, usually polymers, which are degradable by enzymatic or acid/base hydrolysis, or by dissolution. Once inserted into the body, the matrix is acted upon by enzymes and body fluids. The sustained release matrix is chosen desirably from biocompatible materials, including, but not limited to, liposomes, polylactides, polyglycolide (polymer of glycolic acid), polylactide co-glycolide (copolymers of lactic acid and glycolic acid), polyanhydrides, poly(ortho)esters, polypeptides, hyaluronic acid, collagen, chondroitin sulfate, carboxylic acids, fatty acids, phospholipds, polysaccharides, nucleic acids, polyamino acids, amino acids such as phenylalanine, tyrosine, isoleucine, polynucleotides, polyvinyl propylene, polyvinylpyrrolidone and silicone. A preferred biodegradable matrix is a matrix of one of either polylactide, polyglycolide, or polylactide co-glycolide.
  • The dosage of the composition will depend on the condition being treated, the particular composition used, and other clinical factors, such as weight and condition of the patient, and the route of administration. A suitable dosage may range from 100 to 0.1 mg/kg. A more preferred dosage may range from 50 to 0.2 mg/kg. A more preferred dosage may range from 25 to 0.5 mg/kg. Tablets or other forms of media may contain from 1 to 1000 mg of the substituted ethylene diamine. Dosage ranges and schedules of administration similar to ethambutol or other anti-tuberculosis drugs may be used.
  • The composition may be administered in combination with other compositions and procedures for the treatment of other disorders occurring in combination with mycobacterial disease. For example, tuberculosis frequently occurs as a secondary complication associated with -acquired immunodeficiency syndrome (AIDS). Patients undergoing AIDS treatment, which includes procedures such as surgery, radiation or chemotherapy, may benefit from the therapeutic methods and compositions described herein.
  • The following specific examples will illustrate the invention as it applies to the particular synthesis of the substituted ethylene diamine compounds, and the in vitro and in vivo suppression of the growth of colonies of M. tuberculosis. In addition, the teachings of R. Lee et al. J. Comb. Chem. 2003, 5, 172-187 are hereby incorporated by reference in their entirety. It will be appreciated that other examples, including minor variations in chemical procedures, will be apparent to those skilled in the art, and that the invention is not limited to these specific illustrated examples.
  • Example 1 Generating the Diamine Library from Commercially Available Amino Alcohol Pre-Loaded Resins
  • General Methods: All reagents were purchased from Sigma-Aldrich. Amino alcohol pre-loaded resins were purchased from NovaBiochem. Solvents acetonitrile, dichloromethane, dimethylformamide, ethylene dichloride, methanol, and tetrahydrofuran were purchased from Aldrich and used as received. Solid phase syntheses were performed on Quest 210 Synthesizer (Argonaut Technologies) and combinatorial chemistry equipment (Whatman Polyfiltronics and Robbins Scientific). Evaporation of the solvents was done using SpeedVac AES (Savant). Mass spectra data were obtained by Electrospray Ionization technique on Perkin Elmer/Sciex, API-300, TQMS with an autosampler.
  • Scheme 2. Description of the Process.
  • The acylation step was carried out in 5 ml tubes using the Quest 210 Synthesizer. Removal of the FMOC group, reductive alkylation reaction with carbonyl compounds, the reduction with Red-Al, and the cleavage from the solid support were carried out in 96-deep (2 ml) well, chemically resistant plates.
  • Step 1. Acylation of Amino Alcohol Pre-Loaded Resins with Amino Acids.
  • Each tube was loaded 0.150 g of corresponding resin (coverage of 0.3-1.0 mmol/g), and all resins were pre-swollen in DCM for 1.5 h and filtered. If Fmoc protected resin was used: the resin was stirred with 2.5 ml of 20% solution of piperidine in DMF for 10 min, filtered, and washed with 2.5 ml of DMF. The procedure was repeated, but the stirring time was 20 min. After that all resins were filtered, washed with DMF (1×2.5 ml) and DCM (2×3 ml). Each tube was charged with 1 ml of dichloromethane. Amino acids, 0.38 mmol in 1 ml of DMF (2.5 mol excess to loaded resin) were mixed with HATU, 0.3 mmol, 011 g in 0.5 ml of DMF (2 mol excess to loaded resin) and allowed to stay for 15-20 min. Then 1.5 ml of mixture acid-HATU were added to each tube following by the addition of solution of 1.5 mmol, 0.26 ml (10 mol excess to loaded resin) of EtNiPr2 in 0.5 ml of dichloromethane. Reaction carried out 8 h at 45° C. and 6-8 h at room temperature. After the reaction was complete, the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1×3 ml), dichloromethane (1×3 ml), and acylation was repeated with the same amount of reagents. At the end, the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1×3 ml), methanol (3×3 ml), sucked dry (on Quest) for 30 min and transferred into vials (one resin per vial), and dried in a desiccator under vacuum for 1 h. After this step all resins were subjected for quality control using MS spectra.
  • Step 2. Alkylation of the Amino Group.
  • Deprotection. Ten prepared resins from the first three steps were pooled together, leaving approximately 0.03 g of each in the individual vials for all necessary deconvolutions. A suspension of the resin mixture (0.08-1.0 g) in 100 ml of a 2:1 mixture of dichloromethane and THF was distributed into two 96-well filterplates and filtered using a filtration manifold. The reaction plates were transferred into combiclamps, and 0.2 ml of 20% solution of piperidine in DMF was added to remove Fmoc protecting group and allowed to stay for 10 min. After 10 min plate was filtered, washed with 0.2 ml of DMF, and deprotection was repeated with 0.2 ml of 20% solution of piperidine in DMF and allowed to stay for 20 min. After that plate was filtered, washed with DMF (0.2 ml per well) and dichloromethane (2×0.5 ml per well).
  • Reaction with the carbonyl compounds. Each well in row A on the reaction plate was charged with 0.1 ml of dichloromethane, 0.08 ml of ˜1.0M solution of appropriate acid in DMF from master plate, 0.05 ml DMF solution of PyBrop, (0.012 g, 0.025 mmol, 2.5 mol excess to loaded resin) and 0.05 ml of EtNiPr2 in dichloromethane (0.017 ml, 0.10 mmol, 10 mol excess to loaded resin). Each well in rows B through H was charged with 0.1 ml of THF, 0.160 ml of ˜1.0 M solution of appropriate aldehyde or ketone in DMF from master plate and allowed to react for 30 min. After 30 min 0.075 ml (0.075 mmol) of 1.0 M solution of NaBCNH3 in THF were added. The reaction plates were sealed and kept at RT for 72 h. At the end, the resins were filtered, washed with THF, DCM (1×1 ml), methanol (2×1 ml) and dried in desiccator under vacuum for 3 h.
  • Step 3. Reduction with Red-AL.
  • The reaction plates were placed into combiclamps. A 1:6 mixture of Red-Al (65+w % in toluene) and THF was added, 0.6 ml per well (0.28 mmol of Red-Al per well), and allowed to react for 4 h. After the reaction completion the resins were filtered, washed with THF (2×1 ml), methanol (3×1 ml) and dried in the filtration manifold.
  • Step 4. Cleavage.
  • This step was carried out using a cleavage manifold. The reaction plates (placed on the top of the collection plates in this manifold) were charged with a 10:88:2 mixture of TFA, dichloromethane, and triisopropylsilane, 0.5 ml per well. After 15 min, the solutions were filtered and collected into proper wells of the collection plates. The procedure was repeated. Solvents were evaporated on a speedvac, and the residual samples were ready for testing.
  • Scheme 3. Description of the Process.
  • The reductive alkylation step of 4-aminobutan-1 of resin and the acylation step were carried out in 5 ml tubes using the Quest 210 Synthesizer. Removal of the FMOC group, reductive alkylation reaction with carbonyl compounds, the reduction with Red-Al, and the cleavage from the solid support were carried out in 96-deep (2 ml) well, chemically resistant plates.
  • Step 1. Reductive Alkylation of 4-Aminobutan-1-ol resin.
  • A suspension of the resin (coverage of 0.3-1.0 mmol/g), 1.0 g (up to 1.0 mmol), in 30 ml of 2:1 mixture of dichloromethane and THF was distribution into 10 tubes, 3 ml per tube and filtered, than each tube was charged with 0.10 g of resin. Resin was pre-swollen in DCM for 1.5 h and filtered. Each tube were loaded with 1.5 ml 1,2-dichloroethane, 0.3 mmol (3 mol excess) of corresponded aldehyde or ketone (alkylating reagent) and allowed to react for 30 min. After that 0.3 mmol (0.3 ml) of 1 M solution of NaBCNH3 in THF were added and reaction was carried out at RT for 48 h. When reaction was completed, all tubes were filtered, washed with THP (2×3 ml), MeOH (3×3 ml) and sucked dry (on Quest for ˜30min).
  • Step 2. Acylation with Amino Acids.
  • All tubes were pre washed with DCM twice. Each tube was charged with 1 ml of dichloromethane. Amino acids, 0.25 mmol in 1 ml of DMF (2.5 mol excess to loaded resin) were mixed with HATU, 0.2 mmol, 0.076 g in 0.5 ml of DMF (2 mol excess to loaded resin) and allowed to stay for 15-20 min. Then 1.5 ml of mixture acid-HATU were added to each tube following by the addition of solution of 1.0 mmol, 0.17 ml (10 mol excess to loaded resin) of EtNiPr2 in 0.5 ml of dichloromethane. Reaction carried out 8 h at 45° C. and 6-8 h at room temperature. After 16 h the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1×3 dichloromethane (1×3 ml) and acylation was repeated with the same amount of reagents. At the end, the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (1×3 ml), methanol (3×3 ml), sucked dry (on Quest) for 30 min and transferred into vials (one resin per vial), and dried in a desiccator under vacuum for 1 h. After this step all resins were subjected for quality control using MS spectra.
  • All Following Reaction Steps,—Alkylation of the Amino Group (Step 3), Reduction with Red-Al (Step 4), and Cleavage (Step 5),—Were Carried as They were Described for the Scheme 2 of this Application.
  • Example 2 Deconvolution
  • Deconvolution of the active wells was performed by re-synthesis of discrete compounds, from the archived FMOC-protected-aminoacetamide resins (10 resins, 0.05-0.10 g each), which were set aside at the end of the acylation step before the pooling. Each resin was assigned a discrete column (1, or 2, or 3, etc.) in a 96-well filterplate, and was divided between X rows (A, B, C, etc), where X is the number of hits discovered in the original screening plate. To each well, in a row, a selected carbonyl compound (present in the hit) was added along with other required reagents:
  • the first selected carbonyl compound was added to the resins in the row “A”, the second carbonyl compound—to the resins in the row “B”, the third carbonyl compound—to the resins in the row “C”, etc. A lay-out of a representative 96-well deconvolution plate is shown in Table 3, FIG. 12.
  • The reaction plates were sealed and kept at RT for 72 h. At the end, the resins were filtered, washed with THF, DCM (1×1 ml), methanol (2×1 ml) and dried in desiccator under vacuum for 2 h. Reduction and cleavage were performed according to steps 5 and 6 of the synthetic protocol. The product wells from the cleavage were analyzed by ESI-MS (Electrospray Ionization Mass Spectroscopy) to ensure the identity of the actives, and were tested in the MIC assay.
  • Solid Phase Synthesis of Selected Substituted Ethylenediamines Using the Quest 210 Synthesizer
  • The solid phase protocol described above for generating a library of diamine compounds was applied to the scale-up synthesis of the selected substituted ethylenediamine compounds. Here, all reaction steps, from the acylation of the commercially available amino alcohol pre-loaded resins to the cleavage of the final product, were carried out using Quest instrument only, which allowed for up to twenty parallel reactions. Purification of all crude samples was done by Flash Chromatography on CombiFlash (Isco, Inc.) to yield desirable products in purity greater than 90%. Here, the synthesis of one of the active compounds, 1-(2-{[3-(4-chlorophenoxy)benzyl]amino}-3-phenylpropyl)piperidin-4-ol, is described below as an example.
  • Figure US20100273826A1-20101028-C00004
  • The Preparation of 1-(2-{[3-(4-chlorophenoxy)benzyl]amino}-3-phenylpropyl)piperidin-4-ol, compound 588
  • Figure US20100273826A1-20101028-C00005
  • Representative Synthesis of Active Compounds.
  • Removal of FMOC protective group. Commercially available resin (N-FMOC-piperidinyl-4-oxy)-(4-methoxyphenyl)methyl polystyrene, coverage (linker) 0.88 mmol/g (0.4 g, 0.35 mmol), was placed into one of the 10 ml tubes of Quest 210 Synthesizer, A solution of piperidine (1.5 ml) in DMF (6 ml) was added and stirred for 30 min, filtered, washed with DMF (1×6 ml), and the addition of piperidine was repeated. The resin was washed with DMF (1×8 ml) and DCM (2×8 ml).
  • Acylation with FMOC protected L-Phenylalanine. The resin was pre-washed with 5 ml of DCM for 20 min. FMOC L-Phenylalanine. (0.0341 g. 0.88 mmol) in 1 ml of DMF (2.5 mol excess to loaded resin) were mixed with HATU (0.33 g, 0.88 mmol) in 3 ml of DMF, and added to the tube following by the addition of solution of 0.6 ml of EtNiPr2. Reaction was carried at RT for 20 h. After the reaction was complete, the resin was filtered, washed with 1:1 mixture of DMF and dichloromethane (1×6 ml), dichloromethane (1×6 ml), and acylation was repeated with the same amount of reagents. At the end, the resins were filtered, washed with 1:1 mixture of DMF and dichloromethane (2×6 ml).
  • Removal of FMOC protective group. A solution of piperidine (1.5 ml) in DMF (6 ml) was added to the resin and formed suspension was stirred for 30 min, filtered, washed with DMF (1×6 ml), and the addition of piperidine was repeated. The resin was washed with DMF (1×8 ml) and methanol (2×8 ml), and sucked dry under Ar for 20 min.
  • Reaction with a carbonyl compound. The resin was pre-washed with THF for 30 min, filtered, and charged with 6 ml of THF. 3-(4-Chlorophenoxy)-benzaldehyde (0.280 ml, 1.00 mmol) was added followed by addition of 1.0 M solution of NaBCNH3 in THF (1 ml, 1 mmol) after 30 min. The reaction was allowed to proceed at RT for 72 h. At the end, the resin was filtered, washed with THF (1×6 ml) and MeOH (2×6 ml), and dried under Ar for 30 min.
  • Reduction with Red-Al. The resulted resin in a tube was pre-washed with anhydrous THF (2×6 ml) and filtered. The tube was charged with 5 ml of anhydrous THF followed by addition upon stirring commercially available Red-Al as 65+% in toluene (1 ml, 3.2 mmol). After 4 h the resin was filtered, washed with THF (2×1 ml) and MeOH (3×1 ml) (addition of MeOH should proceed with caution!), and dried under Ar for 10 min.
  • Cleavage. For this last step of the synthesis the tube with the resin was charged with DCM (8 ml) and TFA (1 ml) and formed bright red suspension was allowed to stir for 30 min. The resin was filtered and the filtrate was collected into a collection tube. The procedure was repeated. DCM and excess of TFA were evaporated on a speedvac. Crude 1-(2-{[3-(4-chlorophenoxy)benzyl]amino}-3-phenylpropyl)piperidin-4-ol (in a form of trufluoroacetate salt) was purified by Flash Chromatography on CombiFlash (Isco) using following conditions: pre-loaded silica gel column, 12 g, flow 15 ml/min, 25 min run, gradient starting with DCM finishing up with DCM/MeOH/NH4OH (600/400/10). Obtained: 0.128 mg of 1-(2-{[3-(4-chlorophenoxy)benzyl]amino}-3-phenylpropyl)piperidin-4-ol ditrifluoroacetate, 53% yield, of at least 95% purity. Mass spectrum (ESI) m/z (MH)+ 451.2, 453.2.

Claims (4)

1-7. (canceled)
8. A method of treating a mycobacterial disease in a human or an animal comprising administering to a human or an animal having a mycobacterial disease an effective disease-treating amount of a compound selected from:
Figure US20100273826A1-20101028-C00006
or salts thereof.
9. A method of treating a mycobacterial disease in a human or an animal comprising administering to a human or an animal having a mycobacterial disease an effective disease-treating amount of a compound selected from:
Figure US20100273826A1-20101028-C00007
Figure US20100273826A1-20101028-C00008
Figure US20100273826A1-20101028-C00009
Figure US20100273826A1-20101028-C00010
Figure US20100273826A1-20101028-C00011
Figure US20100273826A1-20101028-C00012
Figure US20100273826A1-20101028-C00013
Figure US20100273826A1-20101028-C00014
Figure US20100273826A1-20101028-C00015
Figure US20100273826A1-20101028-C00016
or salts thereof.
10. A method of treating a mycobacterial disease in a human or an animal comprising administering to a human or an animal having a mycobacterial disease an effective disease-treating amount of a compound selected from:
Figure US20100273826A1-20101028-C00017
Figure US20100273826A1-20101028-C00018
Figure US20100273826A1-20101028-C00019
Figure US20100273826A1-20101028-C00020
or salts thereof.
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Citations (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2483252A (en) * 1945-04-20 1949-09-27 Commercial Solvents Corp Method for preparing hydroxylammonium salts of organic acids
US2623878A (en) * 1948-02-09 1952-12-30 Ciba Pharm Prod Inc 2, 4-disubstituted amino quinazolines
US2627491A (en) * 1950-07-15 1953-02-03 Wyeth Corp Penicillin salts of substituted alkylene diamines
US2709169A (en) * 1955-05-24 X c chs
US2767161A (en) * 1956-07-02 1956-10-16 Bristol Lab Inc Dehydroabietyl-ethylenediamine
US2876236A (en) * 1952-08-26 1959-03-03 American Home Prod Heterocyclic diamines and salts thereof
US3176040A (en) * 1961-02-07 1965-03-30 American Cyanamid Co Novel 2, 2'-(ethylenediimino)-di-1-butanols
US3197468A (en) * 1963-02-18 1965-07-27 Lepetit Spa Rifamycin b hydrazides
US3553257A (en) * 1966-09-16 1971-01-05 American Cyanamid Co Preparation of d-2-amino-1-butanol salts
US3579587A (en) * 1968-04-18 1971-05-18 Zoja Lab Chim Farm Process for the preparation of the dextrorotatory 2,2' - (ethylene-di-imino)-di-1-butanol
US3579586A (en) * 1968-06-06 1971-05-18 Zoja Lab Chim Farm Process for the preparation of the dextrorotatory 2,2' - (ethylene-di-imino)-di-1-butanol
US3629333A (en) * 1969-08-28 1971-12-21 Du Pont Polymethylenebis admantane amines
US3682922A (en) * 1969-01-16 1972-08-08 Searle & Co N-acyl-n-{8 (n{40 ,n{40 -disubstituted amino)-alkyl{9 -1-adamantylmethylamines
US3718655A (en) * 1969-07-28 1973-02-27 Ferrer Labor Certain diisoniazid methane sulfonate complexes
US3769347A (en) * 1971-02-11 1973-10-30 American Cyanamid Co Production of d,d'-2,2'-(ethylenediimino) di-1-butanol hydrochloride
US3789073A (en) * 1970-04-22 1974-01-29 Squibb & Sons Inc Adamantylalkylaminoalkyl benzamides
US3829493A (en) * 1972-06-09 1974-08-13 Pliva Pharm & Chem Works Process for preparing d-n,n'-bis-(1-hydroxymethylpropyl)-ethylene diamine
US3847991A (en) * 1971-12-30 1974-11-12 Farmaceutici Italia Process for the preparation of ethambutol(2,2'-(ethylene diamino)-di-butane-1-ol)
US3855300A (en) * 1973-09-05 1974-12-17 Sankyo Chem Ind Ltd Process for the production of 2-amino-1-butanol
US3876702A (en) * 1968-04-26 1975-04-08 Rudolf Theodor Petersen N,n-bis-(diphenylalkyl)-alkylendiamine and their salts
US3878201A (en) * 1971-04-05 1975-04-15 American Cyanamid Co 1,5-Bis substituted-1,4-pentadien-3-one substituted amidinohydrazone salts and method of preparing the same
US3931157A (en) * 1974-12-18 1976-01-06 American Cyanamid Company Substituted 2-benzofuranyl propenones and method of preparation
US3931152A (en) * 1974-01-29 1976-01-06 American Cyanamid Company 2-(1,3-Diazacycloalkenyl)-2-hydrazones of substituted chalcones
US3944617A (en) * 1976-02-20 1976-03-16 American Cyanamid Company Synthesis of dl-2-amino-1-butanol
US3944616A (en) * 1974-10-29 1976-03-16 American Cyanamid Company Purification of d,d'-2,2'(ethylenediimino)di-1-butanol dihydrochloride
US3953513A (en) * 1973-11-29 1976-04-27 Gruppo Lepetit S.P.A. Process for preparing α-aminoalcohols
US3979457A (en) * 1973-09-28 1976-09-07 Denki Kagaku Kogyo Kabushiki Kaisha Process for production of (+)-2-amino-1-butanol
US4006234A (en) * 1974-12-18 1977-02-01 American Cyanamid Company Substituted 2-benzofuranyl propenones as anti-tubercular agents
USRE29358E (en) * 1973-03-01 1977-08-16 American Cyanamid Company 1,5-Bis substituted-1,4-pentadien-3-one substituted amidino hydrazone salts and method of preparing the same
US4150030A (en) * 1975-12-22 1979-04-17 American Cyanamid Company 3-Acyl-4-ethyl-2-oxazolones and oxazolidinones
US4204998A (en) * 1974-03-28 1980-05-27 Siegfried Aktiengesellschaft N-Amino indole derivatives having pharmacological activity
US4262122A (en) * 1980-02-19 1981-04-14 American Cyanamid Company Preparation of 5,5-dimethyl-2-hydrazino-1,4,5,6-tetrahydro-pyrimidine hydrohalide
US4450274A (en) * 1981-07-07 1984-05-22 Korean Advanced Institute Of Science And Technology Preparation of ethambutol-diisoniazide methane sulfonic acid salt
US4457931A (en) * 1982-09-27 1984-07-03 Selvi & C. S.P.A. Piperazine derivatives with anticholinergic and/or antihistaminic activity
US5104875A (en) * 1989-05-19 1992-04-14 Fatol Arzneimittel Gmbh Combination preparations containing rifampicin and thioacetazon
US5256391A (en) * 1992-09-11 1993-10-26 Mobil Oil Corporation Method for synthesizing microporous crystalline material
US5439891A (en) * 1993-10-29 1995-08-08 Kapil; Randhir S. Process for preparation of pharmaceutical composition with enhanced activity for treatment of tuberculosis and leprosy
US5864045A (en) * 1995-03-15 1999-01-26 Hoechst Marion Roussel, Inc. Heterocyclic substituted piperazinone derivatives
US5922282A (en) * 1995-06-07 1999-07-13 Ledley; Robert S. Super fast tuberculosis diagnosis and sensitivity testing method
US5985935A (en) * 1992-03-06 1999-11-16 Statens Seruminstitut Treatment and prophylaxis of diseases caused by parasites, or bacteria
US6300061B1 (en) * 1992-02-07 2001-10-09 Albert Einstein College Of Medicine Of Yeshiva University Mycobacterial species-specific reporter mycobacteriophages
US20020007524A1 (en) * 2000-03-17 2002-01-24 Novozymes A/S Method for dyeing dry hair
US20030069204A1 (en) * 1998-07-09 2003-04-10 Sankyo Company Limited Strain of streptomyces
US20030171330A1 (en) * 1999-08-20 2003-09-11 Sankyo Company, Limited Antibacterial compound
US6660744B1 (en) * 1999-09-17 2003-12-09 Abbott Gmbh & Co. Kg Pyrazolopyrimidines as therapeutic agents
US20030236225A1 (en) * 2002-05-17 2003-12-25 Protopopova Marina Nikolaevna Methods of use and compositions for the diagnosis and treatment of infectious disease
US20040033986A1 (en) * 2002-05-17 2004-02-19 Protopopova Marina Nikolaevna Anti tubercular drug: compositions and methods
US20040058964A1 (en) * 2002-02-14 2004-03-25 Balekudru Devadas Substituted pyridinones
US20040147591A1 (en) * 2001-06-08 2004-07-29 Mitsubishi Chemical Corporation Azasugar compound
US20050014800A1 (en) * 2001-11-30 2005-01-20 Hidehito Matsuoka Angiogenesis inhibitor
US20050113574A1 (en) * 2003-09-05 2005-05-26 Elena Bogatcheva Methods and compositions comprising diamines as new anti-tubercular therapeutics
US7456222B2 (en) * 2002-05-17 2008-11-25 Sequella, Inc. Anti tubercular drug: compositions and methods

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR913169A (en) 1941-12-10 1946-08-30 Rhone Poulenc Sa Process for preparing arylaliphatic diamines
US2483434A (en) 1946-04-08 1949-10-04 Parke Davis & Co Disubstituted amino-alkyl benzhydryl amines
FR1067944A (en) 1951-08-21 1954-06-21 Bayer Ag Production of substituted 4-aminotoluenes
FR915M (en) 1960-03-14 1961-11-06
GB961317A (en) 1960-06-02
GB919177A (en) 1960-09-14 1963-02-20 Ici Ltd Substituted ethylenediamines
NL6410363A (en) 1964-09-05 1966-03-07
GB1157143A (en) 1966-06-21 1969-07-02 Du Pont Adamantane Derivatives
DE1768297B1 (en) 1968-04-26 1971-12-16 Beiersdorf Ag Substituted alkylenediamines and their salts
GB1234349A (en) 1969-03-21 1971-06-03
SU803348A1 (en) 1979-10-12 1981-09-30 Предприятие П/Я В-2343 Oxyethylammonium derivatives of adamantane possessing antivirus activity
SU805605A1 (en) 1979-10-12 1981-09-30 Предприятие П/Я В-2343 N-(1-adamantylmethyl-)-2-chloroacetamide as semiproduct for synthesis of hydrohalides of n-(2-oxyethyl)-aminoalkyl derivatives of 1-adamantylmethylamine
CA1157774A (en) 1980-07-30 1983-11-29 Anthony Cerami Method for the reduction of mucin viscosity
FI95572C (en) 1987-06-22 1996-02-26 Eisai Co Ltd Process for the preparation of a medicament useful as a piperidine derivative or its pharmaceutical salt
US4985560A (en) 1990-01-12 1991-01-15 American Home Products Corporation Pyridazino(4,5-b)indolizines
CA2093633A1 (en) 1991-08-23 1993-02-24 Seiji Sato Carbostyril derivative and platelets aggregation inhibitory agent
AU2681392A (en) 1991-11-12 1993-06-15 Pfizer Inc. Acyclic ethylenediamine derivatives as substance p receptor antagonists
US5576355A (en) 1993-06-04 1996-11-19 Mobil Oil Corp. Diamondoid derivatives for pharmaceutical use
WO1998005332A1 (en) 1996-08-01 1998-02-12 Isis Pharmaceuticals, Inc. Novel heterocycle compositions
MXPA00009705A (en) 1998-04-03 2003-07-14 Univ Manitoba The use of polyamines in the treatment of dermatological symptoms.
US6262066B1 (en) 1998-07-27 2001-07-17 Schering Corporation High affinity ligands for nociceptin receptor ORL-1
KR100665204B1 (en) 1998-12-23 2007-01-04 세라밴스 인코포레이티드 Glycopeptide derivatives and pharmaceutical compositions containing the same
GB0012874D0 (en) 2000-05-27 2000-07-19 Ufc Limited 4-Aminoquinolines
DE60140495D1 (en) 2000-05-31 2009-12-24 Santen Pharmaceutical Co Ltd 1-((adamantyl) alkyl-3-((pyridinyl) alkyl urea compounds as TNF-α-alpha inhibitors for the treatment of autoimmune diseases
RU2168986C1 (en) 2000-08-03 2001-06-20 Открытое акционерное общество "Химико-фармацевтический комбинат "Акрихин" Pharmaceutical composition showing antituberculosis effect
AU2003202115A1 (en) 2002-02-12 2003-09-04 Pfizer Inc. Non-peptide compounds affecting the action of gonadotropin-releasing hormone (gnrh)

Patent Citations (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2709169A (en) * 1955-05-24 X c chs
US2483252A (en) * 1945-04-20 1949-09-27 Commercial Solvents Corp Method for preparing hydroxylammonium salts of organic acids
US2623878A (en) * 1948-02-09 1952-12-30 Ciba Pharm Prod Inc 2, 4-disubstituted amino quinazolines
US2627491A (en) * 1950-07-15 1953-02-03 Wyeth Corp Penicillin salts of substituted alkylene diamines
US2876236A (en) * 1952-08-26 1959-03-03 American Home Prod Heterocyclic diamines and salts thereof
US2767161A (en) * 1956-07-02 1956-10-16 Bristol Lab Inc Dehydroabietyl-ethylenediamine
US3176040A (en) * 1961-02-07 1965-03-30 American Cyanamid Co Novel 2, 2'-(ethylenediimino)-di-1-butanols
US3197468A (en) * 1963-02-18 1965-07-27 Lepetit Spa Rifamycin b hydrazides
US3553257A (en) * 1966-09-16 1971-01-05 American Cyanamid Co Preparation of d-2-amino-1-butanol salts
USRE29588E (en) * 1966-09-16 1978-03-21 American Cyanamid Company Preparation of d-2-amino-1-butanol salts
US3579587A (en) * 1968-04-18 1971-05-18 Zoja Lab Chim Farm Process for the preparation of the dextrorotatory 2,2' - (ethylene-di-imino)-di-1-butanol
US3876702A (en) * 1968-04-26 1975-04-08 Rudolf Theodor Petersen N,n-bis-(diphenylalkyl)-alkylendiamine and their salts
US3579586A (en) * 1968-06-06 1971-05-18 Zoja Lab Chim Farm Process for the preparation of the dextrorotatory 2,2' - (ethylene-di-imino)-di-1-butanol
US3682922A (en) * 1969-01-16 1972-08-08 Searle & Co N-acyl-n-{8 (n{40 ,n{40 -disubstituted amino)-alkyl{9 -1-adamantylmethylamines
US3718655A (en) * 1969-07-28 1973-02-27 Ferrer Labor Certain diisoniazid methane sulfonate complexes
US3629333A (en) * 1969-08-28 1971-12-21 Du Pont Polymethylenebis admantane amines
US3789073A (en) * 1970-04-22 1974-01-29 Squibb & Sons Inc Adamantylalkylaminoalkyl benzamides
US3769347A (en) * 1971-02-11 1973-10-30 American Cyanamid Co Production of d,d'-2,2'-(ethylenediimino) di-1-butanol hydrochloride
US3878201A (en) * 1971-04-05 1975-04-15 American Cyanamid Co 1,5-Bis substituted-1,4-pentadien-3-one substituted amidinohydrazone salts and method of preparing the same
US3847991A (en) * 1971-12-30 1974-11-12 Farmaceutici Italia Process for the preparation of ethambutol(2,2'-(ethylene diamino)-di-butane-1-ol)
US3829493A (en) * 1972-06-09 1974-08-13 Pliva Pharm & Chem Works Process for preparing d-n,n'-bis-(1-hydroxymethylpropyl)-ethylene diamine
USRE29358E (en) * 1973-03-01 1977-08-16 American Cyanamid Company 1,5-Bis substituted-1,4-pentadien-3-one substituted amidino hydrazone salts and method of preparing the same
US3855300A (en) * 1973-09-05 1974-12-17 Sankyo Chem Ind Ltd Process for the production of 2-amino-1-butanol
US3979457A (en) * 1973-09-28 1976-09-07 Denki Kagaku Kogyo Kabushiki Kaisha Process for production of (+)-2-amino-1-butanol
US3953513A (en) * 1973-11-29 1976-04-27 Gruppo Lepetit S.P.A. Process for preparing α-aminoalcohols
US3931152A (en) * 1974-01-29 1976-01-06 American Cyanamid Company 2-(1,3-Diazacycloalkenyl)-2-hydrazones of substituted chalcones
US4204998A (en) * 1974-03-28 1980-05-27 Siegfried Aktiengesellschaft N-Amino indole derivatives having pharmacological activity
US3944616A (en) * 1974-10-29 1976-03-16 American Cyanamid Company Purification of d,d'-2,2'(ethylenediimino)di-1-butanol dihydrochloride
US4006234A (en) * 1974-12-18 1977-02-01 American Cyanamid Company Substituted 2-benzofuranyl propenones as anti-tubercular agents
US3931157A (en) * 1974-12-18 1976-01-06 American Cyanamid Company Substituted 2-benzofuranyl propenones and method of preparation
US4150030A (en) * 1975-12-22 1979-04-17 American Cyanamid Company 3-Acyl-4-ethyl-2-oxazolones and oxazolidinones
US3944619A (en) * 1976-02-20 1976-03-16 American Cyanamid Company Synthesis of d-2-amino-1-butanol
US3944617A (en) * 1976-02-20 1976-03-16 American Cyanamid Company Synthesis of dl-2-amino-1-butanol
US3944608A (en) * 1976-02-20 1976-03-16 American Cyanamid Company Synthesis of N-[1-(chloromethyl)propyl]acetamide
US4262122A (en) * 1980-02-19 1981-04-14 American Cyanamid Company Preparation of 5,5-dimethyl-2-hydrazino-1,4,5,6-tetrahydro-pyrimidine hydrohalide
US4450274A (en) * 1981-07-07 1984-05-22 Korean Advanced Institute Of Science And Technology Preparation of ethambutol-diisoniazide methane sulfonic acid salt
US4457931A (en) * 1982-09-27 1984-07-03 Selvi & C. S.P.A. Piperazine derivatives with anticholinergic and/or antihistaminic activity
US5104875A (en) * 1989-05-19 1992-04-14 Fatol Arzneimittel Gmbh Combination preparations containing rifampicin and thioacetazon
US6300061B1 (en) * 1992-02-07 2001-10-09 Albert Einstein College Of Medicine Of Yeshiva University Mycobacterial species-specific reporter mycobacteriophages
US5985935A (en) * 1992-03-06 1999-11-16 Statens Seruminstitut Treatment and prophylaxis of diseases caused by parasites, or bacteria
US5256391A (en) * 1992-09-11 1993-10-26 Mobil Oil Corporation Method for synthesizing microporous crystalline material
US5380947A (en) * 1992-09-11 1995-01-10 Mobil Oil Corporation Diamondoid-containing amines
US5439891A (en) * 1993-10-29 1995-08-08 Kapil; Randhir S. Process for preparation of pharmaceutical composition with enhanced activity for treatment of tuberculosis and leprosy
US5864045A (en) * 1995-03-15 1999-01-26 Hoechst Marion Roussel, Inc. Heterocyclic substituted piperazinone derivatives
US5922282A (en) * 1995-06-07 1999-07-13 Ledley; Robert S. Super fast tuberculosis diagnosis and sensitivity testing method
US20030069204A1 (en) * 1998-07-09 2003-04-10 Sankyo Company Limited Strain of streptomyces
US20030171330A1 (en) * 1999-08-20 2003-09-11 Sankyo Company, Limited Antibacterial compound
US6660744B1 (en) * 1999-09-17 2003-12-09 Abbott Gmbh & Co. Kg Pyrazolopyrimidines as therapeutic agents
US20020007524A1 (en) * 2000-03-17 2002-01-24 Novozymes A/S Method for dyeing dry hair
US20040147591A1 (en) * 2001-06-08 2004-07-29 Mitsubishi Chemical Corporation Azasugar compound
US20050014800A1 (en) * 2001-11-30 2005-01-20 Hidehito Matsuoka Angiogenesis inhibitor
US20040058964A1 (en) * 2002-02-14 2004-03-25 Balekudru Devadas Substituted pyridinones
US20040033986A1 (en) * 2002-05-17 2004-02-19 Protopopova Marina Nikolaevna Anti tubercular drug: compositions and methods
US20030236225A1 (en) * 2002-05-17 2003-12-25 Protopopova Marina Nikolaevna Methods of use and compositions for the diagnosis and treatment of infectious disease
US7456222B2 (en) * 2002-05-17 2008-11-25 Sequella, Inc. Anti tubercular drug: compositions and methods
US7842729B2 (en) * 2002-05-17 2010-11-30 The United States Of America As Represented By The Department Of Health And Human Services Anti tubercular drug: compositions and methods
US20050113574A1 (en) * 2003-09-05 2005-05-26 Elena Bogatcheva Methods and compositions comprising diamines as new anti-tubercular therapeutics

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