US20080279833A1 - Laminate sheet articles for tissue regeneration - Google Patents

Laminate sheet articles for tissue regeneration Download PDF

Info

Publication number
US20080279833A1
US20080279833A1 US11/747,004 US74700407A US2008279833A1 US 20080279833 A1 US20080279833 A1 US 20080279833A1 US 74700407 A US74700407 A US 74700407A US 2008279833 A1 US2008279833 A1 US 2008279833A1
Authority
US
United States
Prior art keywords
cell
article
sheets
extracellular matrix
tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US11/747,004
Inventor
Robert G. Matheny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cormatrix Cardiovascular Inc
Original Assignee
Matheny Robert G
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matheny Robert G filed Critical Matheny Robert G
Priority to US11/747,004 priority Critical patent/US20080279833A1/en
Publication of US20080279833A1 publication Critical patent/US20080279833A1/en
Assigned to CORMATRIX CARDIOVASCULAR, INC. reassignment CORMATRIX CARDIOVASCULAR, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MATHENY, ROBERT
Priority claimed from US13/573,566 external-priority patent/US9066993B2/en
Priority claimed from US14/452,794 external-priority patent/US9700654B2/en
Priority claimed from US14/571,679 external-priority patent/US20150100120A1/en
Application status is Abandoned legal-status Critical

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54Biologically active materials, e.g. therapeutic substances
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61FFILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02Prostheses implantable into the body
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18Growth factors; Growth regulators
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18Growth factors; Growth regulators
    • A61K38/1825Fibroblast growth factor [FGF]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3629Intestinal tissue, e.g. small intestinal submucosa
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3633Extracellular matrix [ECM]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3679Hollow organs, e.g. bladder, esophagus, urether, uterus, intestine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L27/00Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
    • A61L31/00Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/005Ingredients of undetermined constitution or reaction products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61FFILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02Prostheses implantable into the body
    • A61F2/24Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61FFILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2210/00Particular material properties of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
    • A61F2210/0076Particular material properties of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof multilayered, e.g. laminated structures
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/22Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37Digestive system
    • A61K35/38Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/005Enzyme inhibitors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0679Cells of the gastro-intestinal tract
    • C12N5/068Stem cells; Progenitors

Abstract

The invention is to articles of extracellular matrix. The articles comprise one or more sheets of mammalian extracellular matrix laminated together. A single sheet can be folded over and laminated on 3 sides. Two or more sheets can be laminated to each other at their edges. The sheets can further encase a composition comprising a cell or cells, such as for example, a stem cell. A single sheet can be folded over to encase a composition, or rolled to encase a composition with lamination at either end of the roll, for example. The invention also includes methods of using these articles to regenerate tissue at tissue defects, or heal wounds in damaged tissue.

Description

    CROSS-REFERENCES TO RELATED APPLICATIONS
  • The present application is not related to any other applications.
  • FIELD OF THE INVENTION
  • The invention relates to articles and compositions having two or more forms of extracellular matrix.
  • BACKGROUND OF THE INVENTION
  • Tissue regeneration has been accomplished by using extracellular matrix material derived from mammalian tissues. Some of these mammalian tissues that have been described in patent literature include small intestine submucosa (SIS), liver basement membrane (LBM), urinary bladder submucosa (UBS) and stomach submucosa (SS). See U.S. Pat. No. 5,554,389, U.S. Pat. No. 4,902,508, and U.S. Pat. No. 5,281,422. Enamel matrices, which are the extracellular matrix around forming teeth, are described in U.S. Pat. No. 7,033,611. Extracellular matrices from these tissues have been isolated and dried to become solid materials (sheets and particulates). Particulate forms can be rehydrated in a suitable buffer to become fluidized or emulsive forms. Presently, these extracellular matrix compositions are used for tissue grafting, wound healing, and tissue regenerative purposes. (cite WSJ article)
  • It would be advantageous to the field of tissue engineering to invent articles and compositions for effecting improved tissue regeneration.
  • SUMMARY OF THE INVENTION
  • The invention is an article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix.
  • The invention is an article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix, said article further comprising in between at least two of said sheets at least one cell to further effect wound healing or tissue regeneration upon placement of said laminated article in a mammal at a site in said mammal in need of wound healing or tissue regeneration.
  • In the article the cell can be a stem cell. The cell can be a mesechymal cell.
  • The invention is also a method comprising: identifying a defect or wound in mammalian tissue which could benefit from tissue regeneration or wound healing, providing an article comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix, contacting said defect or wound with said article, and regenerating tissue at said defect or healing said wound thereby.
  • BRIEF DESCRIPTION OF THE DRAWINGS
  • FIG. 1 depicts laminate sheets of extracellular matrix forming a laminate extracellular matrix article.
  • FIG. 2 depicts an article having two sheets of extracellular matrix encasing a composition comprising a cell or a plurality of cells.
  • FIG. 3 depicts an article having a single sheet of extracellular matrix that is folded over a composition comprising a cell or a plurality of cells.
  • DETAILED DESCRIPTION OF THE INVENTION
  • The invention is an article made of extracellular matrix for placing in a mammal at a site in need of tissue regeneration or wound healing to cause tissue regeneration and wound healing. These articles are made from extracellular matrices that are derived from one or more than one tissue source in one or more donor mammals.
  • The article is a laminate of two or more sheets of extracellular matrix. Accordingly, two components of such an article are first and second sheets of extracellular matrix, that are laminated together to form a laminate of extracellular matrix sheets. The two sheets in this example can be from the same source of extracellular matrix, ie. both or all from SIS from a pig. The sheets can also be from different tissue sources of extracellular matrix, for example the first sheet is SIS, and the second sheet is SS. Both the SIS and SS can be from the same species of mammal (e.g. pig) or each from a different species of mammal (SIS from pig, and SS from cow). If there are 3 sheets in the laminate article all 3 can be SIS, or the first sheet can be SIS, the second SS, and the third sheet can be SIS, for example. These three sheets can be from the same species of mammal, i.e. a pig, or different mammalian species, i.e. the SIS sheets can be from a pig and the SS sheet can be from a cow.
  • Advantages are to be derived from using sheets of extracellular matrix from different mammalian tissues, where, for example, each tissue source provides certain attributes. For example, SIS provides tensile strength and the kind of support to newly forming tissue that one would attribute to small intestine submucosa. Adding a sheet from a different tissue, for example one without the tensile strength, but with other regenerative attributes, for example liver basement membrane (LBM), can lend to the article that is a laminate of sheets, an advantageous quality, particularly when two such sheets are laminate together. A sandwich configuration of such sheets can be formed, for example with two outer sheets having relatively substantial tensile strength and an inner sheet of something less strong having other attributes, such as LBM. A SIS-LBM-SIS sheet sandwich may provide the appropriate matrix for tissue regeneration for certain tissues in the body having certain requirements both for strength and regenerative potential.
  • The article can be two sheets of extracellular matrix encasing a composition. The composition can be any dispersible composition comprising a cell or cells that can rest upon a sheet of extracellular matrix and be covered (and encased by) another sheet. The composition can comprise a cell or cells, such as for example a plurality of stem cells that can aide and promulgate tissue regeneration from the article after placement in the patient. So then, for example the sheets can be SIS and the composition can comprise LBM, or the sheets can be SIS and the gel composition can also be SIS.
  • For any of these articles, the sheets can be laminated to each other at the edges around an amount of composition (comprising for example cells and other components) that then becomes encased in the two sheets upon lamination of the outer sheets to each other. The lamination of the two outer sheets together can be partial or complete, so that the composition can be entirely contained within the two sheets, or can be permitted to ooze out from between the sheets upon placement in the subject receiving treatment. The composition comprising the cells can also be a composition that supports the cells and allows them to survive and differentiate in that environment.
  • In another embodiment the sheets can encase one or more cells. The cell or cells can be stem cells. The sheet sandwich can act as support for the growth and development of the cells once placed in the body. The cell or cells can advantageously work in the article to regenerate tissue, or heal damaged tissue in conjunction with the extracellular matrix sheets. The cell or cells can be part of a composition comprising such cells, such as cell media or other material that will help promote the cell survival and differentiation.
  • The cell in the composition can be any cell, such as, for example a human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell. This list is not intended to be exhaustive.
  • The composition comprising a cell or cells can comprise any material supportive of the purposes of the article and cell culture, cell survival and differentiation. Thus, for example, the composition can comprise extracellular matrix that supports cells in culture and in vivo. The composition can comprise any material supportive of the purposes of the composition and the article in general, such as for example tissue regeneration, wound healing, cell culturing and survival, cell differentiation, stem cell recruitment and the like.
  • Any composition to support the cells such as an extracellular matrix composition can comprise such forms of extracellular matrix as an emulsion, gel, liquid, paste or particulate placed in between the sheets of matrix can be of mixed source of extracellular matrix, so that for example the gel can be a 50:50 mixture of LBM and UBS. The composition can also be a mixture of LBM and UBS. Thus, the composition can be some mixture or ratio of extracellular matrix from one or more tissue sources.
  • Generally, for any of the articles of the invention, the components such as sheets of extracellular matrix can be from the same mammalian tissue source (e.g. SIS) or they can be from different tissue sources (e.g. a SIS sheet and an LBM emulsion). Mammalian tissue sources are in general any tissue having an extracellular matrix that can be isolated from a mammal and decellularized. Thus for example, most mammalian organs are tissue sources. The tissue sources can be for example any mammalian tissue, including but not limited to the small intestine, large intestine, stomach, lung, liver, kidney, pancreas, placenta, heart, bladder, prostate, tissue surrounding growing tooth enamel, tissue surrounding growing bone, and any fetal tissue from any mammalian organ.
  • The forms of the extracellular matrices that make up the articles are generally sheets, although the sheets can be in any shape or size necessary for the site. Thus, for example the sheets can be square, rectangular, triangular, or circular. The sheets can be large or small, depending once again on the site that the article is to be placed.
  • Placement of the articles in the patients can be accomplished by any reasonable means, including simply placing the article at the site of defect, or attaching the article in place, e.g. by glue or suture.
  • Extracellular matrix can be obtained from the tissues of mammals by processes such as described in U.S. Pat. No. 5,554,389, U.S. Pat. No. 4,902,508, and U.S. Pat. No. 5,281,422. For example, the urinary bladder submucosa is an extracellular matrix that has the tunica mucosa (which includes the transitional epithelial layer and the tunica propria), a submucosal layer, 3 layers of muscularis, and the adventitia (a loose connective tissue layer). This general configuration is true also for small intestine submucosa (SIS) and stomach submucosa (SS). Obtaining enamel matrices is described in U.S. Pat. No. 7,033,611. Enamel matrix is extracellular matrix existing near forming teeth.
  • Other tissues such as the liver and pancreas have a basement membrane that does not demonstrate the kind of tensile strength of the tissues defined as submucosa. However, other useful properties may be opportunistically employed from the extracellular matrices of such tissues as the liver, pancreas, placenta and lung tissues which have either basement membrane for extracellular matrix or interstitial membrane (as with the lung). These softer matrices support cells such as those in the organs from which the matrices are derived. Thus, certain benefits are to be found in using the extracellular matrices of these tissues, especially in combination with other such matrices like SIS and SS that may be stronger and which offer their particular advantages. The extracellular matrices surrounding developing tooth enamel and developing bone also have particular advantages over other matrices in that they support the growth and differentiation of the hard tissues of bone and enamel.
  • Matrices can be used in whole or in part, so that for example, an extracellular matrix can contain just the basement membrane (or transitional epithelial layer) with the sub-adjacent tunica propria, the tunica submucosa, tunica muscularis, and tunica serosa. The matrix composition can contain any or all of these layers, and thus could conceivably contain only the basement membrane portion, excluding the submucosa. However, generally, and especially since the submucosa is thought to contain and support the active growth factors and other proteins necessary for in vivo tissue regeneration, the matrix composition from any given source will contain the active extracellular matrix portions that support cell development and differentiation and tissue regeneration. Thus it is generally understood by persons of skill in the art that the extracellular matrix of any of the mammalian tissue consists of several basically inseparable layers broadly termed extracellular matrix. Where layers can be separated these separate layers can electively be included in the composition, depending on whether they serve the purpose that is the goal of the article being made.
  • The sheets can come from one or more sources of mammalian extracellular matrix. Thus, for example, the composition can comprise extracellular matrix combinations from such sources as, for example but not limited to, small intestine submucosa, liver basement membrane, stomach submucosa, urinary bladder submucosa, placental basement membrane, pancreatic basement membrane, large intestine submucosa, lung interstitial membrane, respiratory tract submucosa, heart extracellular matrix, dermal matrix, and in general extracellular matrix from any mammalian fetal tissue. Generally a given sheet will be of one source of extracellular matrix, but if the article has two sheets, one sheet can be from one tissue source, and the second sheet can be from a second, different, tissue source.
  • The compositions of the invention can be made as follows: cells are selected for seeding and placing in between the sheets of extracellular matrix. The cell media is selected and the cells cultured to viability and then placed in the article.
  • In making the laminates, the ends of the sheets can be sealed using any reasonable means to do so, such as for example gluing or suturing the sheets to each other to form the article. If the sheets are encasing a composition comprising a cell or cells, the sheets are laminated at the outside edges and will encase the cells or cell composition. If a single sheet is folded over to encase a composition, lamination occurs on three sides of the sheet. If a rectangular, or other-shaped article is constructed from two or more sheets in a laminate, lamination occurs at the edges of the article to seal the composition inside, or to affix the sheets together.
  • For example, sheets can be laminated or layered with each other, so that a sheet of SIS can be placed with a sheet of SS, either with two sheets together SIS-SS or as a sandwich with three sheets, for example SIS-SS-SIS. Also, a different sandwich configuration can be made with two sheets of SIS or SS, sandwiching a gelatinous semi-solid or a solid powder (particulate) form of the matrix. The sandwich can be closed so that a composition can be placed securely between the two outer sheets. A single sheet can alternatively be folded over to encase an amount of composition.
  • Turning now to the figures, FIG. 1 depicts the laminate sheets in a rectangle shape, and circular and triangle shapes. FIG. 1A depicts a first rectangular sheet 10, and second rectangular sheet 11, before lamination. FIG. 1B depicts rectangular sheet 10 and rectangular sheet 11 laminated together to form laminated article 12. FIG. 1C depicts laminated article 12, having sheets 10 and 11 laminated together in a 3-dimensional perspective to form rectangular laminated article 12. FIG. 1D depicts circular laminated article 13 having laminated circular sheets 14 and 16 laminated together. FIG. 1E depicts laminated article 15 having a triangular shape, formed by lamination of triangular sheets 17 and 18 being laminated together.
  • FIG. 2A depicts two sheets, a top sheet 20 and a bottom sheet 22, overlaying a composition 24 comprising cells. FIG. 2B depicts a cross sectional view of the top sheet 20 and bottom sheet 22 laminated at point 26 to encase composition 24. FIG. 2C depicts a 3-dimensional view of top sheet 20 and bottom sheet 22 with composition 24 in between the two sheets, ready for lamination. FIG. 2D depicts a circular article having top sheet 30 and bottom sheet 32 with composition 34 in between them, ready for lamination to close the edges and prepare the article for insertion into a mammalian patient.
  • FIG. 3A depicts single sheet 40 encasing composition 42. FIG. 3B depicts single sheet 40 encasing composition 42 having laminated edge 44. FIG. 3C depicts single sheet 40 having composition 42 with laminate points 46 on 3 sides of the article.
  • The laminate article can encase a composition. The composition can comprise a cell or a plurality of cells. The composition can comprise a stem cell or a plurality of stem cells. The composition can be a material that supports the culturing of the cells. The composition can comprise extracellular matrix in gel or emulsion form that supports cell growth and survival.
  • The composition that might be encased in one or two sheets of extracellular matrix in addition to comprising a cell or cells might further comprise an additional component. The additional component can be any component that some how serves the composition and its purpose in the mammalian body. Thus, the additional component can help to regenerate tissue, heal a wound, better cultivate cells in the composition, better recruit endogenous stem cells once in the body, manipulate the immune environment in a beneficial way, therapeutically treat the local environment, or otherwise contribute to some aspect of the process for which the composition and article that includes the composition is being used.
  • Thus, the additional component can be a protein or a drug.
  • The protein can be for example a growth factor, or any other type or protein that might stimulate some part of the tissue regenerative process. a collagen, a proteoglycan, a glycosaminoglycan (GAG) chain, a glycoprotein, a growth factor, a cytokine, a cell-surface associated protein, a cell adhesion molecule (CAM), an angiogenic growth factor, an endothelial ligand, a matrikine, a matrix metalloprotease, a cadherin, an immunoglobin, a fibril collagen, a non-fibrillar collagen, a basement membrane collagen, a multiplexin, a small-leucine rich proteoglycan, decorin, biglycan, a fibromodulin, keratocan, lumican, epiphycan, a heparan sulfate proteoglycan, perlecan, agrin, testican, syndecan, glypican, serglycin, selectin, a lectican, aggrecan, versican, nuerocan, brevican, cytoplasmic domain-44 (CD-44), macrophage stimulating factor, amyloid precursor protein, heparin, chondroitin sulfate B (dermatan sulfate), chondroitin sulfate A, heparan sulfate, hyaluronic acid, fibronectin (Fn), tenascin, elastin, fibrillin, laminin, nidogen/entactin, fibulin I, fibulin II, integrin, a transmembrane molecule, platelet derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-beta), fibroblast growth factor-2 (FGF-2) (also called basic fibroblast growth factor (bFGF)), thrombospondin, osteopontin, angiotensin converting enzyme (ACE), and vascular epithelial growth factor (VEGF). This list is not intended to be exhaustive.
  • The additional component can also be a drug, such as an agent that has therapeutic properties. The drug can be bioactive and play some role in the process of tissue regeneration or act as an antibiotic, antiviral, or other active therapeutic agent serving a purpose in the composition as a whole. The drug can be a small molecule, or any other agent having therapeutic properties.
  • The invention contemplates using the articles of the invention for contacting a defect in mammalian tissue. The defect can be a cut, disease, wound, burn, scar, necrosis, or other abnormality that would be beneficial to treat. Regenerating tissue at the defect can be one response elicited from the step of placing the extracellular matrix composition in contact with the defect. If the defect is a wound in need of healing, wound healing may be another response that occurs as a result of placing the extracellular matrix at the wound site. In general any term that identifies that the tissue could benefit from a healing or tissue regeneration fits within the scope of the use for the composition. Thus regenerating tissue, or healing a wound are two but the not the only phrases that can be used to describe the effects achieved when the composition is placed in the mammal at a site of defect or damage in tissue.
  • Therapeutically effective amount is a term meant to capture the idea that you need to apply enough of the composition in sufficient strength so that the composition can have a positive effect on the tissue that is being treated in the subject. The amount may therefore apply to an amount of cell or cells in the composition encased by the laminate. That the amount is therapeutically effective is determined by the composition's ability to have an effect on the regenerative or wound healing activity provided by the article (that encases the composition) as a whole at the site where the article (and composition) contacts the tissue. A therapeutically effective amount is determinable by routine testing in patients with wounds or defects. In general a minimal therapeutically effective amount would be considered sufficient cells (or sufficient amount of an additional component) in the composition to effect the wound healing or tissue regeneration at the site of placement of the article that contains the cells or the additional component.
  • Regenerating tissue, as is accomplished by placing an article of the invention in a mammal in need of tissue regeneration, is the ability to make tissue regrow, an organ regrow itself, and for tissue to reform or new tissue to form without scarring. Healing a wound is the ability of the tissue to heal preferably without scarring or with very minimal scarring.
  • All references cited are incorporated in their entirety. Although the foregoing invention has been described in detail for purposes of clarity of understanding, it will be obvious that certain modifications may be practiced within the scope of the appended claims.

Claims (20)

1. An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix.
2. The article of claim 1, wherein said planar laminated article comprises two sheets of mammalian extracellular matrix.
3. The article of claim 1, wherein said two or more sheets of mammalian extracellular matrix are from different mammalian tissue sources.
4. The article of claim 3, wherein a first sheet is from small Intestine submucosa (SIS) of a mammal, and a second sheet is from stomach submucosa (SS) of a mammal.
5. The article of claim 1, wherein said lamination is complete.
6. The article of claim 1, wherein said lamination is partial.
7. The article of claim 1, wherein said sheets are rectangular, and said planar laminated article is rectangular.
8. The article of claim 1, wherein said sheets are triangular, and said planar laminated article is triangular.
9. The article of claim 1, wherein said sheets are circular, and said planar laminated article is circular.
10. An article for wound healing and tissue regeneration comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix, said article further comprising in between at least two of said sheets at least one cell to further effect wound healing or tissue regeneration upon placement of said laminated article in a mammal at a site in said mammal in need of wound healing or tissue regeneration.
11. The article of claim 10, wherein said cell is a stem cell.
12. The article of claim 10, wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell.
13. The article of claim 10, wherein said cell is a mesechymal cell.
14. A method comprising:
a. identifying a defect or wound in mammalian tissue which could benefit from tissue regeneration or wound healing,
b. providing an article comprising two or more sheets of mammalian extracellular matrix, said sheets of mammalian extracellular matrix laminated to each other to form a planar laminated article of mammalian extracellular matrix,
c. contacting said defect or wound with said article, and
d. regenerating tissue at said defect or healing said wound thereby.
15. The method of claim 14, wherein said defect or wound is in myocardial tissue.
16. The method of claim 14, wherein said defect or wound is in pancreatic tissue.
17. The method of claim 14, wherein said defect or wound is in liver tissue.
18. The method of claim 14, wherein said article further comprises a composition comprising a cell between at least two sheets of extracellular matrix.
19. The method of claim 18, wherein said cell is a stem cell.
20. The method of claim 18, wherein said cell is selected from the group consisting of human embryonic stem cell, a fetal cardiomyocyte, a myofibroblast, a mesenchymal stem cell, an autotransplanted expanded cardiomyocyte, an adipocyte, a totipotent cell, a pluripotent cell, a blood stem cell, a myoblast, an adult stem cell, a bone marrow cell, a mesenchymal cell, an embryonic stem cell, a parenchymal cell, an epithelial cell, an endothelial cell, a mesothelial cell, a fibroblast, a myofibroblast, an osteoblast, a chondrocyte, an exogenous cell, an endogenous cell, a stem cell, a hematopoetic stem cell, a pluripotent stem cell, a bone marrow-derived progenitor cell, a progenitor cell, a myocardial cell, a skeletal cell, a fetal cell, an embryonic cell, an undifferentiated cell, a multi-potent progenitor cell, a unipotent progenitor cell, a monocyte, a cardiomyocyte, a cardiac myoblast, a skeletal myoblast, a macrophage, a capillary endothelial cell, a xenogenic cell, an allogenic cell, an adult stem cell, and a post-natal stem cell.
US11/747,004 2007-05-10 2007-05-10 Laminate sheet articles for tissue regeneration Abandoned US20080279833A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/747,004 US20080279833A1 (en) 2007-05-10 2007-05-10 Laminate sheet articles for tissue regeneration

Applications Claiming Priority (17)

Application Number Priority Date Filing Date Title
US11/747,004 US20080279833A1 (en) 2007-05-10 2007-05-10 Laminate sheet articles for tissue regeneration
US12/394,914 US20090238855A1 (en) 2007-05-10 2009-02-27 Laminate sheet articles for tissue regeneration
US13/033,102 US8758448B2 (en) 2007-05-10 2011-02-23 Laminate sheet articles for tissue regeneration
US13/573,566 US9066993B2 (en) 2007-05-10 2012-09-24 Extracellular matrix encasement structures and methods
US14/306,368 US9333277B2 (en) 2007-05-10 2014-06-17 Extracellular matrix (ECM) structures for tissue regeneration
US14/452,794 US9700654B2 (en) 2007-05-10 2014-08-06 Extracellular matrix (ECM) structures for tissue regeneration
US14/571,679 US20150100120A1 (en) 2007-05-10 2014-12-16 Extracellular Matrix Encasement Structures and Methods
US14/571,639 US9744264B2 (en) 2007-05-10 2014-12-16 Extracellular matrix encasement structures and methods
US14/685,714 US20150217026A1 (en) 2007-05-10 2015-04-14 Extracellular Matrix (ECM) Structures for Tissue Regeneration
US14/685,755 US9662418B2 (en) 2007-05-10 2015-04-14 Extracellular matrix encasement structures and methods
US14/818,757 US20150335792A1 (en) 2007-05-10 2015-08-05 Extracellular Matrix Encasement Structures and Methods
US14/819,964 US20150335787A1 (en) 2007-05-10 2015-08-06 Extracellular Matrix Encasement Structures and Methods
US14/833,373 US9662419B2 (en) 2007-05-10 2015-08-24 Extracellular matrix encasement structures and methods
US14/833,354 US9682171B2 (en) 2007-05-10 2015-08-24 Extracellular matrix encasement structures and methods
US14/833,327 US20150359935A1 (en) 2007-05-10 2015-08-24 Extracellular Matrix Encasement Structures and Methods
US14/833,404 US9669133B2 (en) 2007-05-10 2015-08-24 Extracellular matrix encasement structures and methods
US14/833,340 US9636437B2 (en) 2007-05-10 2015-08-24 Extracellular matrix encasement structures and methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/394,914 Continuation US20090238855A1 (en) 2007-05-10 2009-02-27 Laminate sheet articles for tissue regeneration

Publications (1)

Publication Number Publication Date
US20080279833A1 true US20080279833A1 (en) 2008-11-13

Family

ID=39969734

Family Applications (5)

Application Number Title Priority Date Filing Date
US11/747,004 Abandoned US20080279833A1 (en) 2007-05-10 2007-05-10 Laminate sheet articles for tissue regeneration
US12/394,914 Abandoned US20090238855A1 (en) 2007-05-10 2009-02-27 Laminate sheet articles for tissue regeneration
US13/033,102 Active 2027-11-01 US8758448B2 (en) 2007-05-10 2011-02-23 Laminate sheet articles for tissue regeneration
US14/306,368 Active 2027-08-15 US9333277B2 (en) 2007-05-10 2014-06-17 Extracellular matrix (ECM) structures for tissue regeneration
US14/685,714 Pending US20150217026A1 (en) 2007-05-10 2015-04-14 Extracellular Matrix (ECM) Structures for Tissue Regeneration

Family Applications After (4)

Application Number Title Priority Date Filing Date
US12/394,914 Abandoned US20090238855A1 (en) 2007-05-10 2009-02-27 Laminate sheet articles for tissue regeneration
US13/033,102 Active 2027-11-01 US8758448B2 (en) 2007-05-10 2011-02-23 Laminate sheet articles for tissue regeneration
US14/306,368 Active 2027-08-15 US9333277B2 (en) 2007-05-10 2014-06-17 Extracellular matrix (ECM) structures for tissue regeneration
US14/685,714 Pending US20150217026A1 (en) 2007-05-10 2015-04-14 Extracellular Matrix (ECM) Structures for Tissue Regeneration

Country Status (1)

Country Link
US (5) US20080279833A1 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8257434B2 (en) 2007-12-18 2012-09-04 Cormatrix Cardiovascular, Inc. Prosthetic tissue valve
EP2553089A1 (en) * 2010-04-01 2013-02-06 Cryo-Cell International, Inc. Cell therapy to improve wound healing and related compositions and methods
EP2598181A1 (en) * 2010-07-31 2013-06-05 Cook Medical Technologies LLC Methods and systems for generating a tissue pocket in a patient
US8679176B2 (en) 2007-12-18 2014-03-25 Cormatrix Cardiovascular, Inc Prosthetic tissue valve
US8696744B2 (en) 2011-05-27 2014-04-15 Cormatrix Cardiovascular, Inc. Extracellular matrix material valve conduit and methods of making thereof
US8980296B2 (en) 2009-02-18 2015-03-17 Cormatrix Cardiovascular, Inc. Compositions and methods for preventing cardiac arrhythmia
CN105664257A (en) * 2016-03-01 2016-06-15 上海卓阮医疗科技有限公司 Compound soft tissue repairing material for stabilizing repairing region
WO2018089397A3 (en) * 2016-11-08 2018-07-05 W.L. Gore & Associates, Inc. Implantable encapsulation devices

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6666892B2 (en) * 1996-08-23 2003-12-23 Cook Biotech Incorporated Multi-formed collagenous biomaterial medical device
US20080279833A1 (en) * 2007-05-10 2008-11-13 Matheny Robert G Laminate sheet articles for tissue regeneration
US9283302B2 (en) 2011-12-16 2016-03-15 Cormatrix Cardiovascular, Inc. Extracellular matrix encasement structures and methods
US9700654B2 (en) * 2007-05-10 2017-07-11 Cormatrix Cardiovascular, Inc. Extracellular matrix (ECM) structures for tissue regeneration
US9358320B2 (en) 2008-04-25 2016-06-07 Allosource Multi-layer tissue patches
US9480549B2 (en) 2008-04-25 2016-11-01 Allosource Multi-layer tissue patches
EP2575913B1 (en) * 2010-05-25 2018-07-25 Cook Biotech Incorporated Methods, substrates, and systems useful for cell seeding of medical grafts
WO2013009837A1 (en) 2011-07-13 2013-01-17 Amendia, Inc. Spinal implants with stem cells
US9931439B2 (en) * 2011-11-24 2018-04-03 Cook Biotech Incorporated Modifiable medical grafts and related methods and apparatuses
US20150064141A1 (en) 2012-04-05 2015-03-05 The Regents Of The University Of California Regenerative sera cells and mesenchymal stem cells
EP3185888A4 (en) * 2014-08-25 2018-06-20 HLI Cellular Therapeutics, LLC Extracellular matrix compositions
US9238090B1 (en) 2014-12-24 2016-01-19 Fettech, Llc Tissue-based compositions

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US5630640A (en) * 1995-03-06 1997-05-20 Fior; Claude Vehicle with a deflecting device forming a front air screen
US5658329A (en) * 1995-02-14 1997-08-19 Mentor Corporation Filling material for soft tissue implant prostheses and implants made therewith
US5697976A (en) * 1992-06-15 1997-12-16 United States Surgical Corporation Bioabsorbable implant material
US6379710B1 (en) * 1996-12-10 2002-04-30 Purdue Research Foundation Biomaterial derived from vertebrate liver tissue
US20030026788A1 (en) * 1999-10-08 2003-02-06 Ferree Bret A. Use of extracellular matrix tissue to preserve cultured cell phenotype
US20030143207A1 (en) * 2001-10-18 2003-07-31 Livesey Stephen A. Remodeling of tissues and organ
US6656488B2 (en) * 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
US20050042254A1 (en) * 2003-07-16 2005-02-24 Toby Freyman Aligned scaffolds for improved myocardial regeneration
US20060147433A1 (en) * 2003-09-04 2006-07-06 Cook Biotech Incorporated Extracellular matrix composite materials, and manufacture and use thereof
US20060292227A1 (en) * 2005-06-23 2006-12-28 Mcpherson Timothy B Extracellular matrix material particles and methods of preparation
US20070111307A1 (en) * 2002-04-24 2007-05-17 Auger Francois A Method for preparing tissue constructs

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US666892A (en) * 1900-04-10 1901-01-29 Philip Argall Crushing-roll.
US4956178A (en) * 1988-07-11 1990-09-11 Purdue Research Foundation Tissue graft composition
US4902508A (en) * 1988-07-11 1990-02-20 Purdue Research Foundation Tissue graft composition
US5281422A (en) * 1991-09-24 1994-01-25 Purdue Research Foundation Graft for promoting autogenous tissue growth
US5641518A (en) * 1992-11-13 1997-06-24 Purdue Research Foundation Method of repairing bone tissue
US5352463A (en) * 1992-11-13 1994-10-04 Badylak Steven F Tissue graft for surgical reconstruction of a collagenous meniscus and method therefor
US6653291B1 (en) * 1992-11-13 2003-11-25 Purdue Research Foundation Composition and method for production of transformed cells
US5275826A (en) * 1992-11-13 1994-01-04 Purdue Research Foundation Fluidized intestinal submucosa and its use as an injectable tissue graft
US5630840A (en) * 1993-01-19 1997-05-20 Schneider (Usa) Inc Clad composite stent
US5695998A (en) * 1995-02-10 1997-12-09 Purdue Research Foundation Submucosa as a growth substrate for islet cells
US6485723B1 (en) * 1995-02-10 2002-11-26 Purdue Research Foundation Enhanced submucosal tissue graft constructs
US5711969A (en) * 1995-04-07 1998-01-27 Purdue Research Foundation Large area submucosal tissue graft constructs
DE69622548D1 (en) * 1995-04-07 2002-08-29 Purdue Research Foundation West Lafayette Tissue graft for bladder reconstruction
US5554389A (en) * 1995-04-07 1996-09-10 Purdue Research Foundation Urinary bladder submucosa derived tissue graft
US5755791A (en) * 1996-04-05 1998-05-26 Purdue Research Foundation Perforated submucosal tissue graft constructs
AU2808397A (en) * 1996-04-26 1997-11-19 Case Western Reserve University Skin regeneration using mesenchymal stem cells
US8716227B2 (en) * 1996-08-23 2014-05-06 Cook Biotech Incorporated Graft prosthesis, materials and methods
US6666892B2 (en) * 1996-08-23 2003-12-23 Cook Biotech Incorporated Multi-formed collagenous biomaterial medical device
DK1378257T3 (en) * 1996-08-23 2008-08-25 Cook Biotech Inc Colagen-based graft
EP0936930B1 (en) * 1996-11-05 2004-07-28 Purdue Research Foundation Myocardial graft constructs
AU728949C (en) * 1996-12-10 2007-03-29 Purdue Research Foundation Submucosa extracts
US6696270B2 (en) * 1996-12-10 2004-02-24 Purdue Research Foundation Gastric submucosal tissue as a novel diagnostic tool
DK1671604T3 (en) * 1996-12-10 2009-11-09 Purdue Research Foundation Synthetic tissue valve of
AU720274B2 (en) * 1996-12-10 2000-05-25 Purdue Research Foundation Stomach submucosa derived tissue graft
WO1998025546A1 (en) * 1996-12-10 1998-06-18 Cook Biotech, Inc. Tubular grafts from purified submucosa
US6475232B1 (en) * 1996-12-10 2002-11-05 Purdue Research Foundation Stent with reduced thrombogenicity
AU743808B2 (en) * 1998-02-27 2002-02-07 Purdue Research Foundation Submucosa gel compositions
EP1985320B1 (en) * 1998-12-01 2017-06-21 Cook Biotech, Inc. A multi-formed collagenous biomaterial medical device
AT410119T (en) * 1998-12-01 2008-10-15 Univ Washington A device for intravascular embolization
AT424162T (en) * 1999-08-06 2009-03-15 Cook Biotech Inc graft construction Tubular
US20090053279A1 (en) * 1999-12-22 2009-02-26 Badylak Stephen F Tissue regenerative composition
CA2840468C (en) * 2000-01-31 2016-07-26 Cook Biotech Incorporated Stent valves and uses of same
US7033611B2 (en) * 2001-02-23 2006-04-25 Biora Bioex Ab Matrix protein compositions for guided connective tissue growth
WO2003002168A1 (en) * 2001-06-29 2003-01-09 Cook Biotech Incorporated Porous sponge matrix medical devices and methods
AT390151T (en) * 2001-10-26 2008-04-15 Cook Biotech Inc A medical implant with a net-like structure
US20050202058A1 (en) * 2002-05-02 2005-09-15 Hiles Michael C. Cell-seeded extracellular matrix grafts
CA2483683C (en) * 2002-05-02 2013-07-02 Purdue Research Foundation Vascularization enhanced graft constructs
JP2005533535A (en) * 2002-05-02 2005-11-10 パーデュー・リサーチ・ファウンデーションPurdue Research Foundation Graft construct angiogenesis was promoted
US20040043006A1 (en) * 2002-08-27 2004-03-04 Badylak Stephen F. Tissue regenerative composition
GB2426710A (en) * 2002-09-06 2006-12-06 Cook Biotech Inc Submucosa tissue graft construct
US20040191226A1 (en) * 2002-12-04 2004-09-30 Badylak Stephen F. Method for repair of body wall
US20040187877A1 (en) * 2002-12-04 2004-09-30 Badylak Stephen F. Method for repair of liver tissue
CA2565203A1 (en) * 2004-03-31 2005-10-20 Cook Incorporated Ecm-based graft material
WO2005096988A1 (en) * 2004-04-01 2005-10-20 Cook Incorporated A device for retracting the walls of a body vessel with remodelable material
US20070014869A1 (en) * 2005-07-15 2007-01-18 Cormatrix Cardiovascular, Inc. Compositions for reconstruction, replacement or repair of intracardiac tissue
US20070014868A1 (en) * 2005-07-15 2007-01-18 Cormatrix Cardiovascular, Inc. Patch for reconstruction, replacement or repair of the pericardial sac
US8568761B2 (en) * 2005-07-15 2013-10-29 Cormatrix Cardiovascular, Inc. Compositions for regenerating defective or absent myocardium
US20080279833A1 (en) * 2007-05-10 2008-11-13 Matheny Robert G Laminate sheet articles for tissue regeneration
US9034367B2 (en) * 2007-05-10 2015-05-19 Cormatrix Cardiovascular, Inc. Articles for tissue regeneration with biodegradable polymer
US20080279939A1 (en) * 2007-05-10 2008-11-13 Firestone Leigh H Extracellular matrix compositions for tissue regeneration
US20080281419A1 (en) * 2007-05-10 2008-11-13 Matheny Robert G Breast implants and compositions of extracellular matrix
US20090157170A1 (en) * 2007-12-18 2009-06-18 Matheny Robert G Trileaflet Semi-Lunar Prosthetic Tissue Valve
US20090157177A1 (en) * 2007-12-18 2009-06-18 Matheny Robert G Sewing Ring for a Prosthetic Tissue Valve
EP3000472B1 (en) 2009-02-18 2017-04-19 Cormatrix Cardiovascular, Inc. Compositions for preventing atrial and ventricular fibrillation
WO2012018680A1 (en) 2010-07-31 2012-02-09 Global Therapeutics Llc Methods and systems for generating a tissue pocket in a patient

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US5697976A (en) * 1992-06-15 1997-12-16 United States Surgical Corporation Bioabsorbable implant material
US5658329A (en) * 1995-02-14 1997-08-19 Mentor Corporation Filling material for soft tissue implant prostheses and implants made therewith
US5630640A (en) * 1995-03-06 1997-05-20 Fior; Claude Vehicle with a deflecting device forming a front air screen
US6379710B1 (en) * 1996-12-10 2002-04-30 Purdue Research Foundation Biomaterial derived from vertebrate liver tissue
US20030026788A1 (en) * 1999-10-08 2003-02-06 Ferree Bret A. Use of extracellular matrix tissue to preserve cultured cell phenotype
US6656488B2 (en) * 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
US20030143207A1 (en) * 2001-10-18 2003-07-31 Livesey Stephen A. Remodeling of tissues and organ
US20070111307A1 (en) * 2002-04-24 2007-05-17 Auger Francois A Method for preparing tissue constructs
US20050042254A1 (en) * 2003-07-16 2005-02-24 Toby Freyman Aligned scaffolds for improved myocardial regeneration
US20060147433A1 (en) * 2003-09-04 2006-07-06 Cook Biotech Incorporated Extracellular matrix composite materials, and manufacture and use thereof
US20060292227A1 (en) * 2005-06-23 2006-12-28 Mcpherson Timothy B Extracellular matrix material particles and methods of preparation

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8257434B2 (en) 2007-12-18 2012-09-04 Cormatrix Cardiovascular, Inc. Prosthetic tissue valve
US8449607B2 (en) 2007-12-18 2013-05-28 Cormatrix Cardiovascular, Inc. Prosthetic tissue valve
US8679176B2 (en) 2007-12-18 2014-03-25 Cormatrix Cardiovascular, Inc Prosthetic tissue valve
US8980296B2 (en) 2009-02-18 2015-03-17 Cormatrix Cardiovascular, Inc. Compositions and methods for preventing cardiac arrhythmia
EP2553089A1 (en) * 2010-04-01 2013-02-06 Cryo-Cell International, Inc. Cell therapy to improve wound healing and related compositions and methods
EP2553089A4 (en) * 2010-04-01 2013-10-23 Cryo Cell Int Cell therapy to improve wound healing and related compositions and methods
EP2598181A1 (en) * 2010-07-31 2013-06-05 Cook Medical Technologies LLC Methods and systems for generating a tissue pocket in a patient
US8696744B2 (en) 2011-05-27 2014-04-15 Cormatrix Cardiovascular, Inc. Extracellular matrix material valve conduit and methods of making thereof
US8845719B2 (en) 2011-05-27 2014-09-30 Cormatrix Cardiovascular, Inc Extracellular matrix material conduits and methods of making and using same
CN105664257A (en) * 2016-03-01 2016-06-15 上海卓阮医疗科技有限公司 Compound soft tissue repairing material for stabilizing repairing region
WO2018089397A3 (en) * 2016-11-08 2018-07-05 W.L. Gore & Associates, Inc. Implantable encapsulation devices

Also Published As

Publication number Publication date
US8758448B2 (en) 2014-06-24
US9333277B2 (en) 2016-05-10
US20150217026A1 (en) 2015-08-06
US20140336780A1 (en) 2014-11-13
US20120016491A1 (en) 2012-01-19
US20090238855A1 (en) 2009-09-24

Similar Documents

Publication Publication Date Title
Cheng et al. Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering
EP1446015B1 (en) Remodeling of tissues and organs
ES2540242T3 (en) three-dimensional fabric structure
EP2079490B1 (en) Processed ecm materials with enhanced component profiles
US10004827B2 (en) Extracellular matrix-derived gels and related methods
Singelyn et al. Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering
Memon et al. Repair of impaired myocardium by means of implantation of engineered autologous myoblast sheets
Jang et al. 3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair
Ruszczak Effect of collagen matrices on dermal wound healing
Woo et al. The edge effect: current therapeutic options to advance the wound edge
ES2213835T3 (en) Intestinal submucosal tissue graft for the repair of neurological tissue.
Cho et al. Small-diameter blood vessels engineered with bone marrow–derived cells
Eitan et al. Acellular cardiac extracellular matrix as a scaffold for tissue engineering: in vitro cell support, remodeling, and biocompatibility
Shores et al. Skin substitutes and alternatives: a review
Sicari et al. The promotion of a constructive macrophage phenotype by solubilized extracellular matrix
Wang et al. Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells
DK2326336T3 (en) Tissue-supporting structures of the extracellular matrix of a foregut
US20070292401A1 (en) Soft tissue repair and regeneration using stem cell products
Piao et al. Effects of cardiac patches engineered with bone marrow-derived mononuclear cells and PGCL scaffolds in a rat myocardial infarction model
Wolf et al. Biologic scaffold composed of skeletal muscle extracellular matrix
US9308225B2 (en) Compositions and methods for preventing cardiac arrhythmia
Gibbs et al. Autologous full‐thickness skin substitute for healing chronic wounds
AU2009298560B2 (en) Compositions and methods for tissue repair with extracellular matrices
US9199001B2 (en) Compositions for regenerating defective or absent myocardium
Merritt et al. Repair of traumatic skeletal muscle injury with bone-marrow-derived mesenchymal stem cells seeded on extracellular matrix

Legal Events

Date Code Title Description
AS Assignment

Owner name: CORMATRIX CARDIOVASCULAR, INC., CALIFORNIA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:MATHENY, ROBERT;REEL/FRAME:021969/0467

Effective date: 20081125