US20040241140A1

US20040241140A1 - Expression vectors able to elicit improved immune response and methods of using same

Info

Publication number: US20040241140A1
Application number: US10/415,431
Authority: US
Inventors: George Pavlakis; Alexander Gragerov; Barbara Felber
Original assignee: US Department of Health and Human Services
Current assignee: US Department of Health and Human Services
Priority date: 2000-11-01
Filing date: 2001-11-01
Publication date: 2004-12-02
Also published as: JP2007244394A; AU2002228722B2; AU2872202A; JP4580410B2; US20120027792A1; WO2002036806A9; EP1379535A4; AU2007201458B8; AU2007201458A8; JP2004528012A; CA2427257A1; EP1379535A2; WO2002036806A3; CA2427257C; ES2386884T3; US20090227664A1; EP1379535B1; AU2007201458B2; AU2007201458A1; WO2002036806A2

Abstract

The invention relates to nucleic acids (such as DNA immunization plasmids), encoding fusion proteins containing a destabilizing amino acid sequence attached to an amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence. The invention also relates to nucleic acids encoding secreted fusion proteins, such as those containing chemokines or cytokines, and an attached amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased as a result of being attached to the secretory sequence. The invention also relates methods of increasing the immunogenicity of the encoded proteins for use as vaccines or in gene therapy.

Description

I. TECHNICAL FIELD

II. BACKGROUND

Cellular immune responses against human immunodeficiency virus type 1 (HIV-1) and the related simian immunodeficiency virus (SIV) have been shown to play an important role in controlling HIV-1 and SIV infection and in delaying disease progression. Containment of primary HIV-1 infection in infected individuals correlates with the emergence of virus-specific cytotoxic T-lymphocyte (CTL) responses (1, 2, 3). In chronically infected individuals, a high-frequency CTL response against HIV-1 is also correlated with a low viral load and slow disease progression (4, 5). An HIV-1-specific CTL response has also been demonstrated in certain highly exposed seronegative individuals (6, 7, 8). Also, strong HIV-specific proliferative responses, which may be critical for the maintenance of CTL responses, have been identified in long-term nonprogressors (9, 10).

HIV-1 Gag is one of the most conserved viral proteins. Broad, cross-clade CTL responses recognizing conserved epitopes in HIV-1 Gag have been detected in HIV-1 infected people (11, 12), and the development of a safe and effective HIV-1 vaccine may depend on the induction of effective CTL and/or T-helper responses against conserved HIV-1 proteins such as Gag. DNA vaccines have been shown to induce efficient cellular immune responses and protection against a variety of viral, bacterial, and parasitic pathogens in animal models. However, DNA vaccines that could induce potent cellular immune responses against HIV-1 Gag are not yet available.

We have recently demonstrated that by destroying inhibitory sequences in the coding region of HIV-1 gag, we could significantly increase Gag protein expression in primate as well as in mouse cells (13, 14, 15, 16) and dramatically enhance immune repsonse induced by a DNA vaccine (13). Since this new Gag expression vector is Rev/RRE-independent and species-independent, it provides a feasible approach to systematically evaluating the strategies that could lead to the maximum induction of cellular immune responses against HIV Gag molecules in animal models.

Intramuscular (i.m.) administration of a DNA vaccine represents a simple and effective means of inducing both humoral and cellular immune responses (17). There are three potential pathways reponsible for antigen presentation after i.m. injection of DNA. First, muscle cells could take up the DNA, express the encoded protein antigen, and present it to immune cells. Recent data suggest that this pathway is rather unlikely in vivo (18). Second, antigen presenting cells such as dendritic cells attracted to the site of injection may take up the DNA, express the encoded protein, and present it to T and B cells. Third, muscle cells may take up the DNA and express the protein antigen, with the antigen then being transmitted to dendritic cells for presentation. If the second possibility is the case, a protein that is synthesized and degraded in the cytoplasm of dendritic cells would be an excellent target for major histocompatibility complex (MHC) class I presentation and induction of CTL responses. Alternatively, if the third scenario were true, a protein synthesized in the muscle cells that could be targeted efficiently to dendritic cells would induce the best CTL response.

To distinguish among these different possibilities, three different forms of HIV-1 Gag DNA vaccine vectors were constructed and compared for the induction of immune responses. These different forms of Gag included (i) a standard Gag (St-Gag) (also called “WT” gag herein) that assembles into particles, which are efficiently released from cells and become surrounded by host-cell-derived lipid membrane acquired during virus budding; (ii) a cytoplasmic form of Gag (Cy-Gag) that fails to target the plasma membrane and therefore remains in the cytoplasm; and (iii) a secreted form of Gag (Sc-Gag) that is synthesized on the cytoplasmic face of the rough endoplasmic reticulum (ER), transported through the ER and Golgi apparatus, and released as a secreted protein (i.e., not surrounded by a lipid membrane) (19). (Mutant Gag proteins that are not targeted efficiently to the plasma membrane and remain primarily in the cytoplasm were created by destroying the myristylation signal of HIV-1 Gag. Sc-Gag molecules were created by the addition of the t-PA signal peptide sequence to the N terminus of the HIV-1 Gag molecule. This sequence provides a signal for translocation of the secreted protein into the lumen of the ER, for transport through the ER and Golgi apparatus, and for release in the form of Sc-Gag molecules.) (19).

In the study described above, the question of whether targeting HIV-1 Gag to various subcellular compartments could influence the induction of immune responses in DNA-immunized mice was addressed. The results demonstrated that targeting the HIV-1 Gag molecules to different subcellular compartments does indeed influence both the humoral and cellular immune responses that are elicited by i.m. DNA vaccination. Specifically, when these forms of Gag were administered to mice as a DNA vaccine, it was found that the DNA vector encoding the Sc-Gag generated better primary CTL and T-helper responses than did the DNA vector encoding Cy-Gag. Furthermore, the DNA vector encoding the Sc-Gag also generated a higher level of secondary CTL responses than did the DNA vector encoding Cy-Gag after DNA priming and recombinant vaccinia virus-Gag infection. Vaccinia virus titers were notably reduced in the ovaries of mice immunized with Gag DNA vaccine more than 125 days before infection, as compared to the titer in mice that received only the control DNA vector. These data indicated that CD8 ⁺ T-cell memory elicited by DNA vaccination is functionally relevant and provides protective immunity in this system. The DNA vector encoding the Sc-Gag provided better protection against recombinant vaccinia virus-Gag than did the DNA vector encoding Cy-Gag (19).

Another study has shown that altering the cellular location of glycoprotein D (gD) from bovine herpesvirus 1 by DNA vaccine modulates humoral immune response. Although both the secreted and cytosolic forms of gD induced an IgG2a antibody response, the secreted from of gD induced a stronger IgG1 response than IgG2a response (23). Similar results for Sc-Gag and Cy-Gag were observed in the study described above. On the other hand, St-Gag (also called “WT” gag herein), which is competent for forming virus-like particles, induced a predominantly IgG2a antibody repsonse. This latter data is consistent with the idea that location of antigens after DNA immunization could influence the type and potency of humoral immune responses.

Although DNA vaccines alone have been shown to protect against pathogenic challenges in small animals (24), their performance in primates has been generally disappointing. DNA vaccines, even with repeated boosting, induce only moderate immune responses when compared to live-attenuated virus or recombinant virus vaccines. However, recent studies have demonstrated that heterologuous priming-boosting immunization regimens using DNA plus recombinant modified vaccinia virus Ankara vectors can induce strong cellular immune responses and protection against malaria in mice (25), (26) and SIV mac (27), (28) in monkey models. Although T-cell immune responses induced by DNA immunization are moderate, they are highly focused upon a few specific epitopes, because of the small number of other epitopes expressed by this antigen delivery system. A boost with a recombinant vaccinia virus expressing the same antigen presumably stimulates this population of primed memory T cells. Our data showed that pSc-GAG induced higher memory T-cell responses than other Gag expression vectors as measured by ex vivo CTL activity, higher number of CD8 ⁺ IFN-γ-producing cells after stimulation with MEC class I-restricted HIV-1 Gag-specific peptide, and greater protection against recombinant vaccinia virus-Gag infection (19). These Gag expression vectors may be useful for further evaluation of heterologous priming and boosting with a DNA plus viral vector in inducing protective cellular immune responses. Similar strategies could be considered for nonhuman primate models where SIV or simian/human immunodeficiency virus challenge can be evaluated.

There have been several reports regarding the use of t-PA signal peptides in DNA vaccines. In the case of HIV-1 Env DNA vaccine (20), replacing the authentic signal peptide of gp 160 with that of t-PA was intended to overcome the Rev/RRE requirement for Env protein expression (21). Replacing the signal peptide sequences of mycobacterial proteins with that of t-PA in DNA vectors has been shown to correlate with more protection against tuberculous challenge in mice, although CTL responses were not measured (22). DNA vectors containing fusion of t-PA peptide with Plasmodium vivax antigens did not significantly increase antibody production in mice, and cellular immune responses were not evaluated (39). Whether the t-PA signal peptide can enhance the induction of immune responses for cytoplasmic antigens in general by means of a DNA vaccine strategy requires further investigation.

Other reports, concerning potential cancer vaccines, have demonstrated that active immunizations of human patients with idiotypic vaccines elicited antigen-specific CD8 ⁺ T-cell responses and antitumor effects (29). Several alternative preclinical strategies to develop vaccines have been previously reported, including fusion of tumor idiotype-derived single chain Fv (“scFv”) with cytokines and immunogenic peptides such as interleukin (“IL”)-2, IL-4 and granulocyte-macrophage colony-stimulating factor (“GM-CSF”) (30, 31, 32). These fusions of scFv with cytokines, toxin fragments and viral peptides predominantly elicit a humoral response with undetectable activation of cell mediated immunity (see Table 2 of ref. 33). In a different approach, the model antigen is rendered immunogenic in mice by genetically fusing it to a chemokine moiety (33, 34, 35). Potent anti-tumor immunity was dependent on the generation of specific andi-idiotypic antibodies and both CD4+ and CD8+ T cells. These researchers hypothesize that administration of these vaccines as fusion proteins or naked DNA vaccines may allow efficient targeting of antigen-presenting cells in vivo. They also propose that chemokine fusion may represent a novel, general strategy for formulating clinically relevant antigens, such as existing or newly identified tumor and FHV antigens into vaccines for cancer and AIDS, respectively, which elicit potent CD8⁺ T-cell immunity (33). These researchers further state that with regard to HIV vaccine development, it has been shown that HIV cannot enter human cells unless it first binds to two types of cell-surface receptors: CD4 and chemokine receptors. The two major variantly tropic HIV viruses infect cells via CCR5 or CXCR4 co-receptors. Therefore, they state that one may envisage a chemokine fusion vaccine for HIV that would elicit not only T-cell and humoral responses against HIV, but possibly could interfere with the binding of HIV to the respective chemokine receptor, thus blocking infection. Finally, they also propose that their strategy may be further improved by modifying and mutating the chemokine moiety, or replacing it with the viral chemokine-like genes, which would reduce the risk of generation of autoantibodies against native chemokines.

Another strategy designed to enhance the induction of antigen-specific CTL responses involves targeting vaccine antigens directly into the MHC class I antigen-processing pathway, thereby providing more of the peptide epitopes that trigger the CTL response. A signal that targets proteins for proteasomal degradation is the assembly of a polyubiquitin chain attached to an accessible Lys residue in the target protein. One factor that influences the rate at which polyubiquitination occurs is the identity of the N-terminal residue of the target protein, as certain non-met N-termini target proteins for rapid degradation by the 26S proteasome. Townsend and others have shown that such “N-end rule” targeting of antigens can enhance their processing and presentation by the class I pathway in an in vitro setting. (See reference 36).

Proteins with non-Met N termini have been expressed in cells using fusion constructs in which the coding sequence of the target protein is fused in-frame to the C terminus of the coding sequence of ubiquitin. Ubiquitin is normally made in the cell as a polyprotein that is cleaved by ubiquitin hydrolases at the C-terminus of each ubiquitin subunit, giving rise to individual ubiquitin molecules. These same ubiquitin hydrolases will also cleave the ubiquitin target fusion protein at the C terminus of ubiquitin, exposing the N terminus of the target. In a recent study, Tobery and Siliciano generated ubiquitin fusions to HIV-1 nef with either Met or Arg at the N terminus of nef (UbMNef and UbRNef, respectively) (37). In in vitro experiments using vaccinia vectors to express UbMNef and UBRNef, it was shown that although both vectors induced expression of comparable amounts of nef, the form of nef with an Arg residue at the N terminus and a much shorter half-life (t _1/2=15 min vs 10 h). Furthermore, immunization of mice with a vaccinia vector expressing the rapidly degraded UbRNef resulted in the induction of a more vigorous nef-specific CTL response than did immunization with a vaccinia vector expressing the stable UbMNef. Tobery and Siliciano conclude that augmenting nef-specific CTL responses by targeting the antigen for rapid cytoplasmic degradation represents an attractive strategy for vaccination against HIV (37).

In a more recent study, Tobery and Siliciano used the viral protein (HIV-1 nef) as a model tumor-associated antigen to evaluate the in vivo efficacy of the “N-end rule” targeting strategy for enhancing the induction of de novo CTL responses in mice. They state that their results suggest that the “N-end rule” targeting strategy can lead to an enhancement in the induction of CTL that is sufficient to confer protection against a lethal dose of antigen-expressing tumor cells (36).

In sum, to date, DNA vaccines expressing various antigens have been used to elicit immune responses. In many cases this response in polarized or suboptimal for practical vaccination purposes. The present invention demonstrates that combinations of DNA vaccines containing different forms of antigens, as well as administration of the DNA vaccines to different immunization sites, increase the immune response, and hence, are expected to provide practical DNA vaccination procedures.

III. SUMMARY OF THE INVENTION

The invention relates to nucleic acids (including, but not limited to, DNA immunization plasmids), encoding fusion proteins comprising a destabilizing amino acid sequence covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence.

The invention also relates to nucleic acids encoding secreted fusion proteins comprising a secretory amino acid sequence, such as those containing chemokines or cytokines, covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the secretory amino acid sequence.

The invention also relates to products produced by the nucleic acids, e.g., mRNA, polypeptides, and viral particles, as well as vectors and vector systems comprising these nucleic acids. The invention also relates host cells comprising these nucleic acids, vectors, vector systems and/or their products.

The invention also relates to compositions comprising these nucleic acids, vectors, vector systems, products and/or host cells, and methods of using these compositions, either alone or in combination, to stimulate an improved immune response.

The invention also relates to methods of using the same or different nucleic acids, vectors, vector systems, products and/or host cells, or compositions thereof, in different sites to enhance the immune response.

The invention also relates to uses of these nucleic acids, vectors, vector systems, host cells and/or compositions to produce mRNA, polypeptides, and/or infectious viral particles, and/or to induce antibodies and/or cytotoxic and/or helper T lymphocytes.

The invention also relates to the use of these nucleic acids, vectors, vector systems, products and/or host cells, or compositions thereof, in gene therapy or as vaccines.

For example, the invention also relates to the use of these nucleic acid constructs, vectors, vector systems and or host cells for use in immunotherapy and immunoprophylaxis, e.g., as a vaccine, or in genetic therapy after expression, in mammals, preferably in humans. The nucleic acid constructs of the invention can include or be incorporated into lentiviral vectors, vaccinia vectors, adenovirus vectors, herpesvirus vectors or other expression vectors or they may also be directly injected into tissue cells resulting in efficient expression of the encoded protein or protein fragment. These constructs may also be used for in-vivo or in-vitro gene replacement, e.g., by homologous recombination with a target gene in-situ. They may also be used for transfecting cells ex-vivo.

IV. BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. Proliferative responses (shown as stimulation index, SD in mice injected with the indicated vectors or combinations. Vectors are as described in the examples. [0024]
FIG. 2. Proliferative responses (shown as stimulation index, SI) in mice injected two times with the indicated SIV expression plasmids or combinations. Together=injection of 3 DNAs at the same sites; 3 sites=injections of the same DNAs at separate sites. Vectors are as described in the examples. [0025]
FIG. 3. Antibody response in monkeys. Two animals (#585, 587) were injected 4× with 5 mg intramuscularly (“i.m.”) of MCP3p37gag expression vector. Two animals (#626, 628) were given the same DNA mucosally as liposome-DNA preparations. Titers plotted as reciprocal serum dilutions scoring positive in HIV p24 ELIZA tests. [0026]
FIG. 4. Percent of IFNgamma+cells in CD8 population after in vitro stimulation with a gag peptide pool in macaques after three vaccinations with either WT+MCP3; WT+CATE; WT+MCP3+CATE; WT; or no vaccination (“Naïve”). (Note: WT means wild-type gag, also referred to as Standard gag (St-gag) herein; MCP3 means MCP3-gag fusions; CATE means β-catenin-gag fusions). [0027]
FIG. 5. Percent of IFNgamma+ cells in CD8 population after in vitro stimulation with an env peptide pool in macaques after three vaccinations with either WT+MCP3; WT+CATE; WT+MCP3+CATE; WT; or no injection (“Naïve”). (Note: WT means wild type env; MCP3 means MCP3-env fusion; CATE means β-catenin-env fusions). [0028]
FIG. 6. Schematic diagram of the SIV envelope encoding vector CMVkan/R—R-SIVgp160CTE. [0029]
FIG. 7. DNA sequence of the SIV envelope encoding vector CMVkan/R—R-SIVgp160CTE containing a mutated SIV env gene. [0030]
FIG. 8. Nucleotide and amino acid sequence of MCP3-gp160 env (HIV) fusion. [0031]
FIG. 9. Nucleotide and protein sequence of the beta-catenin-gp160 env (HIV) fusion. [0032]
FIG. 10. Western blot of HIV env expression vectors. Optimized vectors for wild type sequence of gp160 ([0033] lanes 1, 2, 3) or the fusions to MCP-3 (lane 6, 9), tPA leader peptide (lane 4, 7) and beta-catenin (lane 5, 8) are shown. Transfections with purified plasmid DNA were performed in human 293 cells and either cell extracts (intracellular) or cell supernatants (extracellular) were loaded on SDS-acrylamide gels, blotted, and probed with anti-HIV env antibodies. The positions of gp120 and gp41 are shown. Open arrow indicates degradation products detected in lane 5. CTE and RTE indicates respective additional posttranscriptional control elements present in some vectors.

V. MODES FOR CARRYING OUT THE INVENTION

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention, as claimed. The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate an embodiment of the invention and, together with the description, serve to explain the principles of the invention. [0034]
The invention relates to nucleic acids (including, but not limited to, DNA immunization plasmids), encoding fusion proteins comprising a destabilizing amino acid sequence covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence. [0035]
The invention also relates to nucleic acids encoding secreted fusion proteins comprising a secretory amino acid sequence, such as those containing chemokines or cytokines, covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the secretory amino acid sequence. [0036]
The invention relates to nucleic acids having sequences encoding fusion proteins containing destabilizing amino acid sequences which increase the immunogenicity of an attached amino acid sequence, and to methods of using compositions comprising these nucleic acids, or combinations thereof, to increase the immunogenicity of the encoded protein(s). This invention also relates to nucleic acids encoding a fusion protein containing MCP-3 amino acid sequences and HIV gag or env, or SIV gag or env, and additional proteins related to vaccinations against non-tumor associated antigens, such as pathogen antigens. The invention also relates to methods of using different immunization sites to increase the immunogenicity of the encoded protein(s). [0037]
One aspect of the invention relates to a nucleic acid construct encoding a fusion protein comprising a destabilization sequence covalently linked to an amino acid sequence containing one or more disease-associated antigen. [0038]
Preferred destabilization sequences are those which target the fusion protein to the ubiquitin proteosomal degradation pathway. More preferably, the destabilization sequence is present in the amino acid sequences selected from the group consisting of c-Myc aa2-120; Cyclin A aa13-91; Cyclin B aa13-91; IkBa aa20-45; β-Catenin aa19-44; c-Jun aa1-67; and c-Mos aa1-35, and functional fragments thereof. [0039]
In one embodiment, the invention relates to nucleic acids comprising sequences which encode polypeptides containing a destabilizing amino acid sequence which increases the immunogenicity of a covalently attached amino acid sequence containing a clinically relevant antigen, such as a disease associated antigen, as compared to its immunogenicity in the absence of the destabilizing amino acid sequence. In another embodiment, the invention relates to nucleic acids encoding secreted fusion proteins, such as those containing immunostimulatory chemokines, such as MCP-3 or IP-10, or cytokines, such as GM-CSF, IL-4 or IL-2. [0040]
In a preferred embodiment, the invention relates to fusion proteins containing MCP-3 amino acid sequences and viral antigens such as HIV gag and env or SIV gag or env. [0041]
The nucleic acid sequences of the constructs of the invention can be synthetic (e.g., synthesized by chemical synthesis), semi-synthetic (e.g., a combination of genomic DNA, cDNA, or PCR amplified DNA and synthetic DNA), or recombinantly produced. The nucleic acid sequences also may optionally not contain introns. The nucleic acid sequence encoding the destabilizing amino acid sequence is preferably linked in frame to the N-terminal of a nucleic acid sequence encoding one or more antigen(s) of interest, or immunogenic epitope(s) thereof. These sequences may optionally be linked by another sequence encoding one or more linker amino acids. [0042]
In addition, nucleic acid sequences encoding more than one antigens of interest, may optionally be operably linked in frame or via an internal ribosomal entry site (IRES), e.g., from picornaviral RNA. An IRES will be used in circumstances that one wants to express two proteins (or antigens) from the same promoter. Using an IRES the expression of the two proteins is coordinated. A further polypeptide encoding sequence may also be present under the control of a separate promoter. Such a sequence may encode, for example, a selectable marker, or further antigen(s) of interest. Expression of this sequence may be constitutive; for example, in the case of a selectable marker this may be useful for selecting successfully transfected packaging cells, or packaging cells which are producing particularly high titers of vector particles. Alternatively or additionally, the selectable marker may be useful for selecting cells which have been successfully infected with nucleic acid sequence and have the sequence integrated into their own genome. [0043]
The constructs of the invention may also encode additional immunostimulation molecules, such as the chemokine MCP-3 exemplified herein, and functional fragments thereof. These immunostimulation molecules may be encoded by nucleic acid sequences as part of the fusion protein expression unit or may be encoded by nucleic acid sequences as part of a separate expression unit. These molecules may also be encoded by sequences present on different nucleic acid constructs, vectors, etc. Immunostimulatory molecules such as cytokines, chemokines or lymphokines are well known in the art. See, e.g., U.S. Pat. No. 6,100,387 which is incorporated by reference herein. See, also, e.g., Biragyn and Kwack (1999) (ref. 34). [0044]
When HIV or SIV antigens are encoded, the nucleic acids of the invention may also contain Rev-independent fragments of genes which retain the desired function (e.g., for antigenicity of Gag or Pol, particle formation (Gag) or enzymatic activity (Pol)), or they may also contain Rev-independent variants which have been mutated so that the encoded protein loses a function that is unwanted in certain circumstances. In the latter case, for example, the gene may be modified to encode mutations (at the amino acid level) in the active site of reverse transcriptase or integrase proteins to prevent reverse transcription or integration. Rev-independent fragments of the gag gene and env gene are described in U.S. Pat. Nos. 5,972,596 and 5,965,726, which are incorporated by reference herein. See also, PCT/JUS00/34985 filed Dec. 22, 2000 (published as WO 01/46408 on Jun. 28, 2001) for the gag gene and FIGS. 6 and 7 herein for the SIV env gene. [0045]
The expression of the proteins encoded by these nucleic acid constructs or vectors after transfection into cells may be monitored at both the level of RNA and protein production. RNA levels are quantitated by methods known in the art, e.g., Northern blots, SI mapping or PCR methods. Protein levels may also be quantitated by methods known in the art, e.g., western blot or ELISA or fluorescent detection methods. A fast non-radioactive ELISA protocol can be used to detect gag protein (DUPONT or COULTER gag antigen capture assay). [0046]
Various vectors are known in the art. See, e.g., U.S. Pat. No. 6,100,387, which is incorporated by reference herein. Preferred vectors considered useful in gene therapy and/or as a vaccine vectors, are lentiviral having, depending on the desired circumstances, [0047]
a) no round of replication (i.e., a zero replication system) [0048]
b) one round of replication, or [0049]
c) a fully replicating system [0050]
Such vectors are described, e.g., in PCT/US00/34985 filed Dec. 22, 2000 (published as WO 01/46408 on Jun. 28, 2001); and U.S. Ser. No. 09/872,733, filed Jun. 1, 2001, which are incorporated by reference herein. [0051]
In a preferred embodiment, a HIV- or SIV-based lentiviral system useful in the invention comprises the following three components: [0052]
1) a packaging vector containing nucleic acid sequences encoding the elements necessary for vector packaging such as structural proteins (except for HIV env) and the enzymes required to generate vector particles, the packaging vector comprising at least a mutated Rev-independent HIV or SIV gag/pol gene; [0053]
2) a transfer vector containing genetic cis-acting sequences necessary for the vector to infect the target cell and for transfer of the therapeutic or reporter or other gene(s) of interest, the transfer vector comprising the encapsidation signal and the gene(s) of interest or a cloning site for inserting the gene(s) of interest; and [0054]
3) a vector containing sequences encoding an element necessary for targeting the viral particle to the intended recipient cell, preferably the gene encoding the G glycoprotein of the vesicular stomatis virus (VSV-G) or amphotrophic MuLV or lentiviral envs. [0055]
In such vectors, when the CMV promoter or other strong, high efficiency, promoter is used instead of the HIV-1 LTR promoter in the packaging vector, high expression of gag, pot, or gag/pol can be achieved in the total absence of any other viral protein. The exchange of the HIV-1 LTR promoter with other promoters is beneficial in the packaging vector or other vectors if constitutive expression is desirable and also for expression in mammalian cells other than human cells, such as mouse cells, in which the HIV-1 promoter is weak. In certain embodiments, the presence of heterologous promoters will also be desired in the transfer vector and the envelope encoding vector, when such vectors are used. [0056]
The antigens of interest, in particular, clinically relevant antigens, are chosen according to the effect sought to be achieved. Preferably, the antigen induces antibodies or helper T-cells or cytotoxic T-cells. [0057]
Amino acids, or antigens, of interest useful the nucleic acid constructs of the invention are described, e.g., in U.S. Pat. No. 5,891,432, which is incorporated by reference herein (see, e.g., Col. 13, In. 20 to Col. 17, In. 67). These antigens include, but are not limited to, disease associated antigens such as tumor-associated antigens, autoimmune disease-associated antigens, infectious disease-associated antigens, viral antigens, parasitic antigens and bacterial antigens. Tumor associated antigens include, but are not limited to, p53 and mutants thereof, Ras and mutants thereof, a Bcr/Abl breakpoint peptide, HER-2/neu, [0058] HPV 2, E6, HPV E7, carcinoembryonic antigen, MUC-1, MAGE-1, MAGE-3, BAGE, GAGE-1, GAGE-2, N-acetylglucosaminyltransferase-V, p15, gp100, MART-1/MelanA, tyrosinase, TRP-1, beta-catenin, MUM-1 and CDK-4, N-acetylglucosaminyltransferase-V, p15, gp100, MART-1/MelanA, tyrosinase, TRP-1, beta-catenin, MUM-1 and CDK-4. HIV or SIV antigens include, but are not limited to Gag, Env, Pol, Nef, Vpr, Vpu, Vif Tat and Rev. In a preferred embodiment of the invention, the HIV Gag-Pol-Tat-Rev-Nef or Tat-Rev-Env-Nef antigens are linked together, but are not active as HIV components.
Nucleic acid constructs of the invention, as well as vectors, vector systems or viral particles containing such nucleic acid constructs, or the encoded proteins may be used for gene therapy in vivo (e.g., parenteral inoculation of high titer vector) or ex vivo (e.g., in vitro transduction of patient's cells followed by reinfusion into the patient of the transduced cells). These procedures are been already used in different approved gene therapy protocols. [0059]
One way of performing gene therapy is to extract cells from a patient, infect the extracted cells with a vector, such as a lentiviral vector, or a viral particle and reintroduce the cells back into the patient. A selectable marker may be used to provide a means for enriching for infected or transduced cells or positively selecting for only those cells which have been infected or transduced, before reintroducing the cells into the patient. This procedure may increase the chances of success of the therapy. Selectable markers may be for instance drug resistance genes, metabolic enzyme genes, or any other selectable markers known in the art. Typical selection genes encode proteins that confer resistance to antibiotics and other toxic substances, e.g., histidinol, puromycin, hygromycin, neomycin, methotrexate, etc., and cell surface markers. [0060]
However, it will be evident that for many gene therapy applications of vectors, such as lentiviral vectors, selection for expression of a marker gene may not be possible or necessary. Indeed expression of a selection marker, while convenient for in vitro studies, could be deleterious in vivo because of the inappropriate induction of cytotoxic T lymphocytes (CTLs) directed against the foreign marker protein. Also, it is possible that for in vivo applications, vectors without any internal promoters will be preferable. The presence of internal promoters can affect for example the transduction titres obtainable from a packaging cell line and the stability of the integrated vector. Thus, single transcription unit vectors, which may be bi-cistronic or poly-cistronic, coding for one or two or more therapeutic genes, may be the preferred vector designed for use in vivo. See, e.g., WO 98/17816. [0061]
Vaccines and pharmaceutical compositions comprising at least one of the nucleic acid sequences, polypeptides, viral particles, vectors, vector systems, or transduced or transfected host cells of the invention and a physiologically acceptable carrier are also part of the invention. [0062]
As used herein, the term “transduction” generally refers to the transfer of genetic material into the host via infection, e.g., in this case by the lentiviral vector. The term “transfection” generally refers to the transfer of isolated genetic material into cells via the use of specific transfection agents (e.g., calcium phosphate, DEAE Dextran, lipid formulations, gold particles, and other microparticles) that cross the cytoplasmic membrane and deliver some of the genetic material into the cell nucleus. [0063]

Pharmaceutical Compositions

The pharmaceutical compositions of the invention contain a pharmaceutically and/or therapeutically effective amount of at least one nucleic acid construct, polypeptide, vector, vector system, viral particle/virus stock, or host cell (i.e., agents) of the invention. If desired, the nucleic acid constructs, polypeptides, viral particles, vectors, vector systems, viral particle/virus stock, or host cells of the invention can be isolated and/or purified by methods known in the art. [0064]
In one embodiment of the invention, the effective amount of an agent of the invention per unit dose is an amount sufficient to cause the detectable expression of the antigen of interest. In another embodiment of the invention, the effective amount of agent per unit dose is an amount sufficient to prevent, treat or protect against deleterious effects (including severity, duration, or extent of symptoms) of the condition being treated. The effective amount of agent per unit dose depends, among other things, on the species of mammal inoculated, the body weight of the mammal and the chosen inoculation regimen, as is well known in the art. The dosage of the therapeutic agents which will be most suitable for prophylaxis or treatment will also vary with the form of administration, the particular agent chosen and the physiological characteristics of the particular patient under treatment. The dose is administered at least once. Subsequent doses may be administered as indicated. [0065]
To monitor the response of individuals administered the compositions of the invention, mRNA or protein expression levels may be determined. In many instances it will be sufficient to assess the expression level in serum or plasma obtained from such an individual. Decisions as to whether to administer another dose or to change the amount of the composition administered to the individual may be at least partially based on the expression levels. [0066]
The term “unit dose” as it pertains to the inocula refers to physically discrete units suitable as unitary dosages for mammals, each unit containing a predetermined quantity of active material (e.g., nucleic acid, virus stock or host cell) calculated to produce the desired effect in association with the required diluent. The titers of the virus stocks to be administered to a cell or animal will depend on the application and on type of delivery (e.g., in vivo or ex vivo). The virus stocks can be concentrated using methods such as centrifugation. The titers to be administered ex vivo are preferably in the range of 0.001 to 1 infectious unit/cell. Another method of generating viral stocks is to cocultivate stable cell lines expressing the virus with the target cells. This method has been used to achieve better results when using traditional retroviral vectors because the cells can be infected over a longer period of time and they have the chance to be infected with multiple copies of the vector. [0067]
For in vivo administration of nucleic acid constructs, vectors, vector systems, virus stocks, or cells which have been transduced or transfected ex vivo, the dose is to be determined by dose escalation, with the upper dose being limited by the onset of unacceptable adverse effects. Preliminary starting doses may be extrapolated from experiments using lentiviral vectors in animal models, by methods known in the art, or may be extrapolated from comparisons with known retroviral (e.g., adenoviral) doses. Generally, small dosages will be used initially and, if necessary, will be increased by small increments until the optimum effect under the circumstances is reached. Exemplary dosages are within the range of 108 up to approximately 5×10[0068] ¹⁵particles.
For vaccinations DNA will be administered either IM in PBS as previously described in liposomes, by intradermal inoculation, electro-injection or other methods. As example, 5 mg per dose IM in macaques (DNA at 1 mg/nl) injected at several different sites was found to produce a good immune response. [0069]
Inocula are typically prepared as a solution in a physiologically acceptable carrier such as saline, phosphate-buffered saline and the like to form an aqueous pharmaceutical composition. [0070]
The agents of the invention are generally administered with a physiologically acceptable carrier or vehicle therefor. A physiologically acceptable carrier is one that does not cause an adverse physical reaction upon administration and one in which the nucleic acids or other agents of the invention are sufficiently soluble to retain their activity to deliver a pharmaceutically or therapeutically effective amount of the compound. The pharmaceutically or therapeutically effective amount and method of administration of an agent of the invention may vary based on the individual patient, the indication being treated and other criteria evident to one of ordinary skill in the art. A nucleic acid construct of the invention is preferably present in an amount which is capable of expressing the encoded protein in an amount which is effective to induce antibodies and/or cytotoxic and/or helper-inducer T lymphocytes. A therapeutically effective amount of a nucleic acid of the invention is one sufficient to prevent, or attenuate the severity, extent or duration of the deleterious effects of the condition being treated without causing significant adverse side effects. The route(s) of administration useful in a particular application are apparent to one or ordinary skill in the art. [0071]
Routes of administration of the agents of the invention include, but are not limited to, parenteral, and direct injection into an affected site. Parenteral routes of administration include but are not limited to intravenous, intramuscular, intraperitoneal and subcutaneous. The route of administration of the agents of the invention is typically parenteral and is preferably into the bone marrow, into the CSF intramuscular, subcutaneous, intradermal, intraocular, intracranial, intranasal, and the like. See, e.g., WO 99/04026 for examples of formulations and routes of administration. [0072]
The present invention includes compositions of the agents described above, suitable for parenteral administration including, but not limited to, pharmaceutically acceptable sterile isotonic solutions. Such solutions include, but are not limited to, saline and phosphate buffered saline for nasal, intravenous, intramuscular, intraperitoneal, subcutaneous or direct injection into a joint or other area. [0073]
In providing the agents of the present invention to a recipient mammal, preferably a human, the dosage administered will vary depending upon such factors as the mammal's age, weight, height, sex, general medical condition, previous medical history and the like. [0074]
The administration of the pharmaceutical compositions of the invention may be for either “prophylactic” or “therapeutic” purpose. When provided prophylactically, the compositions are provided in advance of any symptom. The prophylactic administration of the composition serves to prevent or ameliorate any subsequent deleterious effects (including severity, duration, or extent of symptoms) of the condition being treated. When provided therapeutically, the composition is provided at (or shortly after) the onset of a symptom of the condition being treated. [0075]
For all therapeutic, prophylactic and diagnostic uses, one or more of the agents of the invention, as well as antibodies and other necessary reagents and appropriate devices and accessories, may be provided in kit form so as to be readily available and easily used. [0076]
Where immunoassays are involved, such kits may contain a solid support, such as a membrane (e.g., nitrocellulose), a bead, sphere, test tube, rod, and so forth, to which a receptor such as an antibody specific for the target molecule will bind. Such kits can also include a second receptor, such as a labeled antibody. Such kits can be used for sandwich assays to detect toxins. Kits for competitive assays are also envisioned. [0077]

VI. INDUSTRIAL APPLICABILITY

The nucleic acids of this invention can be expressed in the native host cell or organism or in a different cell or organism. The mutated genes can be introduced into a vector such as a plasmid, cosmid, phage, virus or mini-chromosome and inserted into a host cell or organism by methods well known in the art. In general, the constructs can be utilized in any cell, either eukaryotic or prokaryotic, including mammalian cells (e.g., human (e.g., HeLa), monkey (e.g., Cos), rabbit (e.g., rabbit reticulocytes), rat, hamster (e.g., CHO and baby hamster kidney cells) or mouse cells (e.g., L cells), plant cells, yeast cells, insect cells or bacterial cells (e.g., [0078] E. coli). The vectors which can be utilized to clone and/or express nucleic acid sequences of the invention are the vectors which are capable of replicating and/or expressing the coding sequences in the host cell in which the coding sequences are desired to be replicated and/or expressed. See, e.g., F. Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing Associates and Wiley-Interscience (1992) and Sambrook et al. (1989) for examples of appropriate vectors for various types of host cells. The native promoters for such coding sequences can be replaced with strong promoters compatible with the host into which the coding sequences are inserted. These promoters may be inducible. The host cells containing these coding sequences can be used to express large amounts of the protein useful in enzyme preparations, pharmaceuticals, diagnostic reagents, vaccines and therapeutics.
The constructs of the invention may also be used for in-vivo or in-vitro gene therapy. For example, a construct of the invention will produce an mRNA in situ to ultimately increase the amount of polypeptide expressed. Such polypeptides include viral antigens and/or cellular antigens. Such a constructs, and their expression products, are expected to be useful, for example, in the development of a vaccine and/or genetic therapy. [0079]
The constructs and/or products made by using constructs encoding antigens of interest could be used, for example, in the production of diagnostic reagents, vaccines and therapies for diseases, such as AIDS and AIDS-related diseases. [0080]
For example, vectors expressing high levels of Gag can be used in immunotherapy and immunoprophylaxis, after expression in humans. Such vectors include retroviral vectors and also include direct injection of DNA into muscle cells or other receptive cells, resulting in the efficient expression of gag, using the technology described, for example, in Wolff et al., [0081] Science 247:1465-1468 (1990), Wolff et al., Human Molecular Genetics 1(6):363-369 (1992) and Ulmer et al., Science 259:1745-1749 (1993). Further, the gag constructs could be used in transdominant inhibition of HIV expression after the introduction into humans. For this application, for example, appropriate vectors or DNA molecules expressing high levels of p₅₅ ^gagor p37^gagwould be modified to generate transdominant gag mutants, as described, for example, in Trono et al., Cell 59:113-120 (1989). The vectors would be introduced into humans, resulting in the inhibition of HIV production due to the combined mechanisms of gag transdominant inhibition and of immunostimulation by the produced gag protein. In addition, the gag encoding constructs of the invention could be used in the generation of new retroviral vectors based on the expression of lentiviral gag proteins. Lentiviruses have unique characteristics that may allow the targeting and efficient infection of non-dividing cells. Similar applications are expected for vectors expressing high levels of env.
The following examples illustrate certain embodiments of the present invention, but should not be construed as limiting its scope in any way. Certain modifications and variations will be apparent to those skilled in the art from the teachings of the foregoing disclosure and the following examples, and these are intended to be encompassed by the spirit and scope of the invention. [0082]

EXAMPLE 1

Vectors

DNA vectors expressing antigens of HIV-1 or SIV are used in the examples herein. [0083]
Three different types of plasmids encoding forms of HIV Gag exemplified herein are as follows: [0084]
1) plasmids expressing full gag (p55) or parts of gag (p37) or gag and protease (p55gagpro). P55 produces gag particles that are partially released from the cell. P37 is partially released from the cell but does not form particles. P55gagpro also produces protease, therefore the gag is processed to form p17, p24, p6 and p7; [0085]
2) plasmids expressing the chemokine MCP-3 fused to the N terminus of p55 gag. Since MCP-3 is a secreted protein, the produced fusion protein is also secreted from the mammalian cells after the cleavage of the signal peptide; and [0086]
3) plasmids expressing fusions of gag to sequences conferring efficient proteasomal degratation. [0087]
Similar DNA expression vectors were produced for HIV env protein (see, e.g., FIGS. 8-9), as well as for SIV gag and env proteins. The HIV env plasmids were constructed based on a HIV lade B env sequence and tested for expression. Expression was high in the absence of Rev. (See FIG. 10). Specific vectors, and combinations thereof, are described in more detail below. We also have variations of the vectors that do not contain linker amino acids, or contain fewer amino acids for CATENIN, etc, which are not specifically exemplified herein. Smaller fragments of the secretory sequences, or the destabilization sequence, than those exemplified herein, which maintain the desired function, are in some cases known to exist, or can be identified by routine experimentation. These sequences are also useful in the invention. [0088]
p37gag=HIV plasmid described previously [0089]
MCP3p37gag=as above, plus also contains also the leader sequence of ip10 [0090]
The following is an example for MCP3p37gag: [0091]

The vector pCMVkanMCP3gagp37M1-10 expresses the following MCP3-gag fusion protein (SEQ ID NO: 1):


M N P S A A V I F C L I L L G L S G T Q

(IP10)

GILD (linker)

M A Q P V G I N T S T T C C Y R F I N K K I P K

Q R L E S Y R R T T S S H C P R E A V I F K T K

L D K E I C A D P T Q K W V Q D F M K H L D K

K T Q T P K L (MCP-3)

A S A G A (linker)

G A R A S V L S G G E L D R W E K I R L R P G G

K K K Y K L K H I V W A S R E L E R F A V N P G

L L E T S E G C R Q I L G Q L Q P S L Q T G S E E

L R S L Y N T V A T L Y C V H Q R I E I K D T K

E A L D K I E E E Q N K S K K K A Q Q A A A D T

G H S N Q V S Q N Y P I V Q N I Q G Q M V H Q A

I S P R T L N A W V K V V E E K A F S P E V I P

M F S A L S E G A T P Q D L N T M L N T V G G H

Q A A M Q M L K E T I N E E A A E W D R V H P

V H A G P I A P G Q M R E P R G S D I A G T T S

T L Q E Q I G W M T N N P P I P V G E I Y K R W

I I L G L N K I V R M Y S P T S I L D I R Q G P K

E P F R D Y V D R F Y K T L R A E Q A S Q E V K

N W M T E T L L V Q N A N P D C K T I L K A L G

P A A T L E E M M T A C Q G V G G P G H K A R

V L E F • (p37gagHIV)

CYBp37gag=contains cyclin B destabilizing sequences [0093]
CATEp37gag=contains beta catenin destabilizing sequences [0094]
MOSp37gag=contains mos destabilizing sequences [0095]
SIVMCP3p39=as above for HIV [0096]
SIVCATEp39=as above for HIV [0097]
SIVgagDX is a Rev-independent SIV gag molecular clone. This vector is described in PCT/US00/34985 filed Dec. 22, 2000 (published as WO 01/46408 on Jun. 28, 2001), which is incorporated by reference herein. P39 denotes a DNA sequence encoding SIV Gag p39 (SIV p17+p25). P57 denotes a DNA sequence encoding the complete SIV Gag p57. [0098]
“Gag” denotes DNA sequence encoding the Gag protein, which generates components of the virion core, “Pro” denotes “protease.” The protease, reverse transcriptase, and integrase genes comprise the “pol” gene. In these constructs, “MCP3” denotes MCP-3 amino acids 33-109 linked to IP-10 secretory peptide refered supra (alternatively, it can be linked to its own natural secretory peptide or any other functional secretory signal such as the tPA signal mentioned supra), “CYB” denotes Cyclin B amino acids 10-95, “MOS” denotes C-Mos amino acid 1-35 and “CATE” denotes β-catenin amino acids 18-47. [0099]

Cyclin B nucleic acid sequences and encoded amino acids used in the constructs exemplified herein:


ATGTCCAGTGATTTGGAGAATATTGACACAGGAGT	(SEQ ID NO: 2)

TAATTCTAAAGTTAAGAGTCATGTGACTATTAGGC

GAACTGTTTTAGAAGAAATTGGAAATAGAGTTAC

AACCAGAGCAGCACAAGTAGCTAAGAAAGCTCAG

AACACCAAAGTTCCAGTTCAACCCACCAAAACAA

CAAATGTCAACAAACAACTGAAACCTACTGCTTCT

GTCAAACCAGTACAGATGGAAAAGTTGGCTCCAA

AGGGTCCTTCTCCCACACCTGTCGACAGAGAGATG

GGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAAT

TAGATCGATGGGAAAAAATTCGGTTAAGGCCAGG

GGGAAAGAAGAAGTACAAGCTAAAGCACATCGTA

TG

MetSerSerAspLeuGluAsnIleAspThrGlyVal	(SEQ ID NO: 3)

AsnSerLysValLysSerHisValThrIleArgArg

ThrValLeuGluGluIleGlyAsnArgValThrThr

ArgAlaAlaGlnValAlaLysLysAlaGlnAsnThr

LysValProValGlnProThrLysThrThrAsnVal

AsnLysGlnLeuLysProThrAlaSerValLysPro

ValGlnMetGluLysLeuAlaProLysGlyProSer

ProThrProValAspArgGlu

c-Mos nucleic acid sequences and encoded amino acids used in the constructs exemplified herein: [0101]

ATGCCCGATCCCCTGGTCGACAGAGAG (SEQ ID NO: 4)

MetProAspProLeuValAspArgGlu (SEQ ID NO: 5)

EXAMPLE 2

Construction of Vectors [0102]
In order to design “Gag-destabilized” constructs, a literature search for characterized sequences able to target proteins to the ubiquitin-proteasome degradation pathway gave the following, not necessarily representative, list: [0103]
c-Myc aa2-120 [0104]
Cyclin A aa13-91 [0105]
Cyclin B aa13-91 *we used 10-95 in vectors in examples herein [0106]
IkBa aa20-45 [0107]
b-Catenin aa19-44 *we used 18-47 in vectors in examples herein [0108]
c-Jun aa1-67 [0109]
c-Mos aa1-35 [0110]
We cloned a subset of those degradation sequences from Jurkat cDNA, namely the signals from cyclin B, β-catenin, and c-Mos, using PCR. Both cyclin and catenin primers gave fragments of the expected length, that were cut and cloned into the SalI site of the vectors pCMV37(M1-10)kan, or pCMV55(M1-10)kan, and (Bam version) into the BamHI site of pFREDlacZ. (The p37 and p55 plasmids have the same p37 and p55 sequences disclosed in the patents containing INS-gag sequences (see, e.g., U.S. Pat. No. 5,972,596 and U.S. Pat. No. 5,965,726, which are incorporated by reference herein) but they have a different plasmid backbone expressing kanamycin. pFREDlacZ contains the IE CMV promoter expressing beta galactosidase of [0111] E coli.)
The corresponding plasmids are called: [0112]
pCMV37(M1-10)kan with cyclin B sequence in SalI site pS194 [0113]
pCMV37(M1-10)kan with β-catenin sequence in SalI site pS195 [0114]
pCMV55(M1-10)kan with cyclin B sequence in SalI site pS199 [0115]
pCMV55(M1-10)kan with β-catenin sequence in SalI site pS200 [0116]
pFREDlacZ with cyclin B sequence in BamHI site pS201 [0117]
pFREDlacZ with β-catenin sequence in BamHI site pS202 [0118]

In the case of Mos, the degradation signal consists of five N-terminal amino acids and a lysine approximately 30 amino acids away. A similarly located lysine is present in HIV gag, but not in lacZ. For that reason, oligos covering all five destabilizing amino acids were synthesized (both chains), annealed, and linked to the N-terminus of gag, but not lacZ. There were three versions of MOS sequence:



MOSN5wtUP & MOSN5wtDN	has serine shown to cause
	degradation when
	phosphorylated
MOSN5aspUP & MOSN5aspDN	has Asp for Ser substitution,
	mimicking
	phosphorylation for constitutive
	action
MOSN5argUP & MOSN5argDN	has Arg for Ser substitution,
	allegedly making degradation
	signal inactive

Out of six plasmids planned, we only examined the following: [0120]
pS191 having pCMV37(M1-10)kan with the wild type (“WT”) Mos sequence, but the insert is longer than intended, with an additional copy of the synthetic sequence in reverse; [0121]
pS192 having pCMV37(M1-10)kan with “Asp” Mos sequence in the SalI site; and [0122]
pS197 with pCMV55(M1-10)kan with “Asp” Mos sequence in the SalI site. [0123]

EXAMPLE 3

Preliminary Characterization of the Degradation Signals in the Vectors

The following experiments were conducted for preliminary characterization of the degradation signals in the nucleic acid constructs described above. [0124]
β-Galactosidase activity was measured in transiently transfected HeLa and 293 cells after transfection with either pFREDlacZ or its cyclin B or β-catenin-modified versions (pS201 & 202). Apparent loss of the lacZ activity was interpreted as being indicative of ubiquitination signal-induced protein degradation. [0125]
With modified Gag the following experiments were done to confirm that degradation signals work in the gag context as well. First, p24-gag was measured by ELISA in cellular extracts and supernatants of cells transfected with the modified Gag constructs. Although we obtained evidence of destabilization, in several cases this experiment measuring the total level of p24 antigen was inconclusive. This was probably because, as shown previously, fragments of gag can still score positive in the antigen capture assay procedure. Therefore we looked into how intact the produced proteins were. [0126]
Protein extracts of HeLa or 293 cells transiently transfected with different gag plasmids were run on acrylamide tris-glycine gel, transferred to Immobilon P membrane and stained with anti-HIV antibodies to reveal Gag. These experiments did not show any signs of degradation in HeLa cells, however 293 cells transformed with the cyclin or β-catenin-modified versions of Gag clearly demonstrated the presence of prominent Gag-stained bands of molecular weight smaller than the full-length modified Gag. Such non-full length bands were not observed with the wild type Gag-transfected cells. These finding is consistent with the signal-induced Gag degradation. [0127]
To further examine whether the N-terminal modifications induce Gag degradation, we conducted pulse-chase experiments with transiently transfected 293 cells. One day after transfection the cells were incubated in methionine-free medium to exhaust cellular pools, labeled with [0128] ³⁵S-methionine in the same medium, and chased by adding 1000-fold excess of the cold methionine. Two experiments have been done. One with 1 hour pulse and 12 hours chase, and another with 30 min pulse and 1.5 h chase. The experiments showed that the modified Gag degrades more rapidly than the wild type Gag. Both cyclin B and β-catenin-derived signals worked in destabilizing Gag to a similar extent. Additional experiments were performed with the env constructs-beta catenin fusions, and verified that the fusions were much more unstable after expression in human cells.

EXAMPLE 4

Proliferative Responses of Vectors And Combinations of Vectors

These vectors were tested for protein expression in vitro after transfections in mammalian cells and for immunogenicity in mice and primates (macaques). [0129]
Methods: [0130]
DNA was purified using the Qiagen endotoxin free DNA purification kit. Endotoxin levels were routinely measured and were very low (kinetic-QCL test, Bio-Whittaker gave approximately 1 endotoxin unit/mg of DNA in these preparations). [0131]
Mice were injected intramuscularly with 100 μg of DNA in 100 μl of PBS. Three injections of DNA were given at [0132] days 0 14 and 28. At day 35 mice were sacrificed and their splenocytes assayed for proliferation in the presence of the specific gag antigen. In addition, cytotoxic responses were evaluated by performing standard cytotoxicity assays. The antibody response of the vaccinated mice is also under evaluation using sera obtained from these animals.
For monkey experiments, 5 mg of MCP3gag HIV DNA in 5 ml of phosphate buffered saline (PBS) were injected in several spots intramuscularly in Rhesus macaques, after the animals were sedated. Four injections were given at 0, 2, 4, and 8 weeks. The animals were followed by several assays to assess cellular and humoral immune response. Previous immunizations with gag p37M1-10, described in our previous patent gave only low levels of antibodies. The previous gag construct stimulated cellular immunity well, but not antibodies. [0133]
FIG. 1 shows the proliferative responses (shown as stimulation index, SI) in mice injected with the indicated vectors or combinations of the following vectors containing DNA sequences encoding HIV polypeptides, or polypeptide controls: [0134]
p37gag [0135]
MCP3p37gag [0136]
CYBp37gag [0137]
CATEp37gag [0138]
MOSp37gag=*we used WT Mos in the example herein [0139]
CATE+MCP3=*2 constructs, see above; these are the same plasmids used alone or in combinations [0140]
CATE+MCP3+p37=*3 constructs, see above [0141]
FIG. 2 shows proliferative responses (shown as stimulation index, SI) in mice injected two times with the indicated SIV expression plasmids or combinations. Together=injection of 3 DNAs at the same sites; 3 sites=injections of the same DNAs at separate sites. When the “same sites” were used, all DNAs were mixed and injected at the same body sites in the muscle. When separate sites were used, the DNAs were kept separate and injected at anatomical sites that are separate. This happened every time we immunized the mice, i.e., the 3 DNAs were kept separate and injected at different sites from each other; and different sites of injection were used for each vaccination. [0142]
SIVgagDX [0143]
SIVMCP3p39 [0144]
SIVCATEp39 [0145]
MCP3+CATE+P57 (together) [0146]
MCP3+CATE+P57 (3 sites) [0147]
FIG. 3 shows the antibody response in monkeys. Two animals' (#585, 587) were injected 4× with 5 mg IM of MCP3p37gag expression vector. Two animals (#626, 628) were given the same DNA mucosally as liposome-DNA preparations. Titers plotted as reciprocal serum dilutions scoring positive in anti-HIV p24 Eliza tests. [0148]

Results

We found that MCP-3 fusions to gag dramatically increased the immune response to gag, compared to the unmodified gag vectors ([0149] type 1 as described above), see figures. This property may be in part the result of more efficient gag secretion from the cells, since we have recently shown that secreted gag having the leader sequence of tPA was more efficient in secretion and immunogenicity (Qiu et al, J. Virol. 2000).
In addition, this effect may be mediated by the function of MCP-3 molecule. The magnitude of the response suggests additional effects of MCP-3, in agreement with the reported effects of MCP-3 in inducing immunogenicity against a tumor antigen. Intramuscular injection of this MCP3p37gag in macaques led to the production of high titer anti-gag antibodies. This was not the case with previously tested gag expression vectors, indicating that it is possible to elicit an efficient antibody response in primates by only DNA vaccination. In addition, these results suggest that improved immunogenicity in mice was a satisfactory method to predict increased immunogenicity in primates. We therefore tested several vectors and combinations of vectors in mice, in an effort to identify the best combinations for subsequent experiments in primates. [0150]
We also studied the expression and immunogenicity of vectors that direct the expressed HIV antigens towards proteasome degradation and efficient presentation on the cell surface via the MHC-I class of molecules. MHC-I—restricted immunity is known to be important for anti-viral defenses. MHC-I display intracellularly produced short peptides on cell surface. A change in the composition of the peptides exposed by a cell, signals to the immune system that the cell is abnormal (e.g. virally infected) and should be destroyed. The MHC-I—exposed peptides originate from proteasomal degradation of cellular proteins. We tested the hypothesis that supplying HIV antigens with strong additional ubiquitination signals targeting it for proteasomal degradation would increase its chances for being processed for surface presentation. [0151]
We tested several ubiquitination signals identified within known proteins for conferring rapid degradation after linking them to the N-terminus of HIV Gag. In parallel, the same ubiquitination signals were fused to beta-galactosidase to check for degradation efficiency by the drop in its enzymatic activity. This assay showed that all selected signals enhanced beta-galactosidase degradation. [0152]
The most effective sequence identified by these experiments corresponds to amino acids 18-47 of beta-catenin, a protein involved in Wnt signaling and cell-cell adhesion, whose abundance is controlled by degradation. [0153]
30 aa of Beta-catenin (18-47): [0154]

R K A A V S H W Q Q Q S Y L D S G I (SEQ ID NO: 6)

H S G A T T T A P S L S
Beta-catenin(18-47) added at the N terminus of HIV antigens with initiator AUG Met: [0155]

M R K A A V S H W Q Q Q S Y L D S G (SEQ ID NO: 7)

I H S G A T T T A P S L S
Injecting mice with DNA constructs expressing either HIV-I Gag, or Gag fused with beta-catenin destabilizing domain showed that the latter construct was more immunogenic. Compared with Gag alone, beta-catenin-Gag fusion evoked higher HIV-specific proliferative responses, elevated CTL response, and higher level of CD8+ IFNgamma+ -secreting cells. [0156]
Direct comparisons with other destabilizing sequences showed an overal higher potency of beta-catenin-Gag fusion. Therefore, one surprising conclusion is that, although several sequences increased proteasome processing and protein destabilization, the beta-catenin sequences were much better in inducing an increased immune response. Since the practical outcome of these studies is improved vaccination procedures, we propose the use of preferably the beta-catenin sequences identified here for use in targeting antigens for degradation. [0157]
Another important conclusion came from studies of combinations of vectors expressing different forms of antigens. It was found that combinations showed improved immunogenicity especially when injected in different sites on the same mouse, compared to a mix of DNA vectors injected in the same site. [0158]
We propose that different forms of the antigens trigger qualitatively different immune responses. Therefore, combinations of antigens applied at different sites and also at different times, may increase protective immune response. The results so far support the conclusion that using different forms of DNA sequentially or in combinations but applied at different sites may reproduce the good immunogenicity obtained with other prime-boost vaccine combinations. This will be a dramatic improvement over existing procedures for DNA vaccination in primates, which has been shown to be inefficient, especially for stimulating humoral immunity. [0159]

EXAMPLE 5

Immunogenicity of SIV Gag and SIV Env DNA Vectors in Macaques

On the basis of previous data suggesting that the modified forms of HIV and SIV antigens showed different immune responses after DNA vaccination, we studied the immunogenicity of three different DNA vaccine vectors for SIV gag and SIV env in 12 macaques. The DNAs used are shown in Table 1, below:

TABLE 1


SIV DNA Vectors

	full name:

	gag
1	p57gag	SIVgagDX	WT
3	MCP3gag	SIVMCP3p39	extracellular
5	CATEgag	SIVCATEp39	intra cellular
	env

2	gp160env	pCMVkan/R-R-SIVgp160CTE	WT
4	MCP3env	pCMVkan/MCP3/SIVgp160CTE	extracellular
6	CATEenv	pCMVkan/CATE/SIVgp160CTE	intra cellular

The SIV gag vectors are the same as those used in the mice experiments described in the previous examples above. The SIVenv parent vector has been described in patent application Ser. No. 09/872,733, filed Jun. 1, 2001, which is incorporated by reference herein, as an example of a vector with high levels of expression. The schematic diagram and sequence of this vector are set forth in FIGS. 6 and 7 herein, respectively. The MCP3 and CATE fusion vectors contain the same sequences of MCP3 and CATE described for the gag vectors. [0161]

Three groups of four näive macaques (

groups

1, 2, 3) were immunized intramuscularly with purified DNA preparations in PBS as shown in Table 2:

TABLE 2


DNA Immunization

week:

	0	4	12	24

Group 1:	1, 2, 3, 4	1, 2, 3, 4	1, 2, 3, 4	1, 2, 3, 4
Group 2:	1, 2, 5, 6	1, 2, 5, 6	1, 2, 5, 6	1, 2, 5, 6
Group 3:	1, 2, 3, 4, 5, 6	1, 2, 3, 4, 5, 6	1, 2, 3, 4, 5, 6	1, 2, 3, 4,
				5, 6
Group 4:	5, 6	5, 6	3, 4	3, 4
Group 5:	1, 2	1, 2	1, 2	1, 2

The animals were injected with the indicated DNAs. The total amount of DNA injected each time per animal was kept constant at 3 mg for gag and 3 mg for env. Animals were injected at different sites with the different DNAs. Injections were intramuscularly with the DNA delivered in PBS at 1 mg/ml. The sites of injections were anatomically separate for the different DNAs. [0163]
In addition, four animals (group 4) were immunized first with [0164] DNAs 5 and 6 (i.e., SIV CATE gag and SIV CATE env), and subsequently at weeks 12 and 24 with DNAs 3 and 4 (i.e., SIV MCP3 gag and SIV MCP3 env). Two animals in group5 received the DNAs expressing unmodified, wild-type antigens for gag and env (1 and 2). The animals in groups 4 and 5 had been previously exposed to HIV DNA, but they were näive for SIV antigens, which was verified by immunological assays (Antibody measurements and lymphoproliferative responses to specific antigen stimulation). Despite this, animals in groups 4 and 5 showed early responses to SIV DNA injection, indicating an anamnestic response to SIV antigens. Therefore, the experiment for groups 4 and 5 needs to be repeated with naïve animals for final conclusions. At sequential times during vaccination blood samples were obtained and analyzed for the presence of antibodies, lymphoproliferative responses and cytotoxic T cells.
The antibody titers obtained for gag are as shown in Table 3. The reciprocal of the highest dilution scoring positive in Elisa assays is shown. Empty cells indicate antibody reactivity below 1:50 dilution. [0165]
These results showed that administration of MCP3gag vector is associated with strong antibody response, because 8/8 (100%) of animals receiving MCP3gag (in [0166] Groups 1 and 3) developed high gag antibodies. In contrast, 3/6 (50%) of animals not receiving MCP3gag (in Groups 2 and 5) developed antibodies.
The specific cytotoxic T cell responses against gag and env were evaluated by measuring the number of CD8 cells that produce intracellular IFNgamma or TNFalpha in the presence of gag or env synthetic peptide pools (overlapping 15mers). The values obtained after three DNA vaccinations are shown in FIGS. 4 and 5. It is interesting that the combination of three vectors increased the number of specific IFNgamma-producing cells upon peptide stimulation. It was concluded that the animals receiving all three forms of antigens showed increased antibody response without diminishing cellular immune response. Actually the cellular immune response also showed increased cellular immune response and the results showed statistical significant differences. [0167]
These data indicate the development of a more balanced immune response than previously anticipated by DNA vaccination in macaques, by the combination of different antigen forms. [0168]
[0169] Group 4 responses (not shown above) were also elevated (1.11% and 0.88% for gag and env, respectively), but this needs to be repeated by vaccinating naïve animals.
The mechanism of this increased immunogenicity by the combination of DNA vectors needs to be examined further. Expression and secretion of MCP-3-antigen chimeras may lead to increased protein levels that stimulate efficiently humoral immune responses. The combination of different antigen forms may also promote better activation and coordination of effector cells. [0170]

Table 3 shows SIV gag antibody response for all groups from the time of first immunization.

TABLE 3


Antibody Titers In Monkeys Vaccinated with SIV DNAs (Groups 1-5)

week

animal#

	0	3	4	6	8	12	13	14	24	25

Group1	918L			50		50	800	3200	50	800	12
WT + MCP3	919L								50	50	3
	921L			50					50	50
	922L						800	3200	50	50	3
Group2	920L						200	800	50	50
WT + CATE	923L							200	50	3200	3
	924L
	925L
Group3	926L						50	200	50	3200	3
WT + MCP3 + CATE	927L								50	50
	928L						50	800	50	50	3
	929L						50	200	50	3200	3
Group4	585L	800	800	3200	3200	800	3200	800	800	3200	3
CATE, then	587L		50	50			3200	3200	12800	3200	3
MCP3	626L	800	200	50				50	50	3200	3
	628L								50	50	3
Group5	715L	50			800	200	200	200	50	50	3
WT	716L					800

EXAMPLE 6

Use Of Nucleic Acids of the Invention in Immunoprophylaxis or Immunotherapy

In postnatal gene therapy, new genetic information has been introduced into tissues by indirect means such as removing target cells from the body, infecting them with viral vectors carrying the new genetic information, and then reimplanting them into the body; or by direct means such as encapsulating formulations of DNA in liposomes; entrapping DNA in proteoliposomes containing viral envelope receptor proteins; calcium phosphate co-precipitating DNA; and coupling DNA to a polylysine-glycoprotein carrier complex. In addition, in vivo infectivity of cloned viral DNA sequences after direct intrahepatic injection with or without formation of calcium phosphate coprecipitates has also been described. mRNA sequences containing elements that enhance stability have also been shown to be efficiently translated in [0172] Xenopus laevis embryos, with the use of cationic lipid vesicles. See, e.g., J. A. Wolff, et al., Science 247:1465-1468 (1990) and references cited therein.
It has also been shown that injection of pure RNA or DNA directly into skeletal muscle results in significant expression of genes within the muscle cells. J. A. Wolff, et al., [0173] Science 247:1465-1468 (1990). Forcing RNA or DNA introduced into muscle cells by other means such as by particle-acceleration (N.-S. Yang, et al. Proc. Natl. Acad. Sci. USA 87:9568-9572 (1990); S. R. Williams et al., Proc. Natl. Acad. Sci. USA 88:2726-2730 (1991)) or by viral transduction or in vivo electorporation should also allow the DNA or RNA to be stably maintained and expressed. In the experiments reported in Wolff et al., RNA or DNA vectors were used to express reporter genes in mouse skeletal muscle cells, specifically cells of the quadriceps muscles. Protein expression was readily detected and no special delivery system was required for these effects. Polynucleotide expression was also obtained when the composition and volume of the injection fluid and the method of injection were modified from the described protocol. For example, reporter enzyme activity was reported to have been observed with 10 to 100 μl of hypotonic, isotonic, and hypertonic sucrose solutions, Opti-MEM, or sucrose solutions containing 2 mM CaCl₂and also to have been observed when the 10- to 100-μl injections were performed over 20 min. with a pump instead of within 1 min.
Enzymatic activity from the protein encoded by the reporter gene was also detected in abdominal muscle injected with the RNA or DNA vectors, indicating that other muscles can take up and express polynucleotides. Low amounts of reporter enzyme were also detected in other tissues (liver, spleen, skin, lung, brain and blood) injected with the RNA and DNA vectors. Intramuscularly injected plasmid DNA has also been demonstrated to be stably expressed in non-human primate muscle. S. Jiao et al., [0174] Hum. Gene Therapy 3:21-33 (1992).
It has been proposed that the direct transfer of genes into human muscle in situ may have several potential clinical applications. Muscle is potentially a suitable tissue for the heterologous expression of a transgene that would modify disease states in which muscle is not primarily involved, in addition to those in which it is. For example, muscle tissue could be used for the heterologous expression of proteins that can immunize, be secreted in the blood, or clear a circulating toxic metabolite. The use of RNA and a tissue that can be repetitively accessed might be useful for a reversible type of gene transfer, administered much like conventional pharmaceutical treatments. See J. A. Wolff, et al., [0175] Science 247:1465-1468 (1990) and S. Jiao et al., Hum. Gene Therapy 3:21-33 (1992).
It had been proposed by J. A. Wolff et al., supra, that the intracellular expression of genes encoding antigens might provide alternative approaches to vaccine development. This hypothesis has been supported by a recent report that plasmid DNA encoding influenza A nucleoprotein injected into the quadriceps of BALB/c mice resulted in the generation of influenza A nucleoprotein-specific cytotoxic T lymphocytes (CTLs) and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival. J. B. Ulmer et al., Science 259:1745-1749 (1993). [0176]
Therefore, it appears that the direct injection of RNA or DNA vectors encoding the viral antigen can be used for endogenous expression of the antigen to generate the viral antigen for presentation to the immune system without the need for self-replicating agents or adjuvants, resulting in the generation of antigen-specific CTLs and protection from a subsequent challenge with a homologous or heterologous strain of virus. [0177]
CTLs in both mice and humans are capable of recognizing epitopes derived from conserved internal viral proteins and are thought to be important in the immune response against viruses. By recognition of epitopes from conserved viral proteins, CTLs may provide cross-strain protection. CTLs specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. [0178]
Thus, direct injection of RNA or DNA encoding the viral antigen has the advantage of being without some of the limitations of direct peptide delivery or viral vectors. See J. A. Ulmer et al., supra, and the discussions and references therein). Furthermore, the generation of high-titer antibodies to expressed proteins after injection of DNA indicates that this may be a facile and effective means of making antibody-based vaccines targeted towards conserved or non-conserved antigens, either separately or in combination with CTL vaccines targeted towards conserved antigens. These may also be used with traditional peptide vaccines, for the generation of combination vaccines. Furthermore, because protein expression is maintained after DNA injection, the persistence of B and T cell memory may be enhanced, thereby engendering long-lived humoral and cell-mediated immunity. [0179]

Vectors for the Immunoprophylaxis or Immunotherapy Against HIV-1

In one embodiment of the invention, the nucleic acids of the invention will be inserted in expression vectors containing REV independent expression cassettes using a strong constitutive promoter such as CMV or RSV, or an inducible promoter such as HIV-1. [0180]
The vector will be introduced into animals or humans in a pharmaceutically acceptable carrier using one of several techniques such as injection of DNA directly into human tissues; electroporation (in vivo or ex vivo) or transfection of the DNA into primary buman cells in culture (ex vivo), selection of cells for desired properties and reintroduction of such cells into the body, (said selection can be for the successful homologous recombination of the incoming DNA to an appropriate preselected genomic region); generation of infectious particles containing the gag gene, infection of cells ex vivo and reintroduction of such cells into the body; or direct infection by said particles in vivo. [0181]
Substantial levels of protein will be produced (and rapidly degraded in the situations where destabilization sequences are part of the encoded protein) leading to an efficient stimulation of the immune system. [0182]
In another embodiment of the invention, the described constructs will be modified to express mutated Gag proteins that are unable to participate in virus particle formation. It is expected that such Gag proteins will stimulate the immune system to the same extent as the wild-type Gag protein, but be unable to contribute to increased HIV-1 production. This modification should result in safer vectors for immunotherapy and immunophrophylaxis. [0183]

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Those skilled in the art will recognize that any gene encoding a mRNA containing an inhibitory/instability sequence or sequences can be modified in accordance with the exemplified methods of this invention or their functional equivalents. [0269]
Modifications of the above described modes for carrying out the invention that are obvious to those of skill in the fields of genetic engineering, virology, immunology, medicine, and related fields are intended to be within the scope of the following claims. [0270]
Every reference cited hereinbefore throughout the application is hereby incorporated by reference in its entirety. [0271]
1 14 1 471 PRT Artificial Sequence Description of Artificial Sequencevector pCMVkanMCPgagp37M1-10 MCP3-gag fusion protein 1 Met Asn Pro Ser Ala Ala Val Ile Phe Cys Leu Ile Leu Leu Gly Leu 1 5 10 15 Ser Gly Thr Gln Gly Ile Leu Asp Met Ala Gln Pro Val Gly Ile Asn 20 25 30 Thr Ser Thr Thr Cys Cys Tyr Arg Phe Ile Asn Lys Lys Ile Pro Lys 35 40 45 Gln Arg Leu Glu Ser Tyr Arg Arg Thr Thr Ser Ser His Cys Pro Arg 50 55 60 Glu Ala Val Ile Phe Lys Thr Lys Leu Asp Lys Glu Ile Cys Ala Asp 65 70 75 80 Pro Thr Gln Lys Trp Val Gln Asp Phe Met Lys His Leu Asp Lys Lys 85 90 95 Thr Gln Thr Pro Lys Leu Ala Ser Ala Gly Ala Gly Ala Arg Ala Ser 100 105 110 Val Leu Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 115 120 125 Pro Gly Gly Lys Lys Lys Tyr Lys Leu Lys His Ile Val Trp Ala Ser 130 135 140 Arg Glu Leu Glu Arg Phe Ala Val Asn Pro Gly Leu Leu Glu Thr Ser 145 150 155 160 Glu Gly Cys Arg Gln Ile Leu Gly Gln Leu Gln Pro Ser Leu Gln Thr 165 170 175 Gly Ser Glu Glu Leu Arg Ser Leu Tyr Asn Thr Val Ala Thr Leu Tyr 180 185 190 Cys Val His Gln Arg Ile Glu Ile Lys Asp Thr Lys Glu Ala Leu Asp 195 200 205 Lys Ile Glu Glu Glu Gln Asn Lys Ser Lys Lys Lys Ala Gln Gln Ala 210 215 220 Ala Ala Asp Thr Gly His Ser Asn Gln Val Ser Gln Asn Tyr Pro Ile 225 230 235 240 Val Gln Asn Ile Gln Gly Gln Met Val His Gln Ala Ile Ser Pro Arg 245 250 255 Thr Leu Asn Ala Trp Val Lys Val Val Glu Glu Lys Ala Phe Ser Pro 260 265 270 Glu Val Ile Pro Met Phe Ser Ala Leu Ser Glu Gly Ala Thr Pro Gln 275 280 285 Asp Leu Asn Thr Met Leu Asn Thr Val Gly Gly His Gln Ala Ala Met 290 295 300 Gln Met Leu Lys Glu Thr Ile Asn Glu Glu Ala Ala Glu Trp Asp Arg 305 310 315 320 Val His Pro Val His Ala Gly Pro Ile Ala Pro Gly Gln Met Arg Glu 325 330 335 Pro Arg Gly Ser Asp Ile Ala Gly Thr Thr Ser Thr Leu Gln Glu Gln 340 345 350 Ile Gly Trp Met Thr Asn Asn Pro Pro Ile Pro Val Gly Glu Ile Tyr 355 360 365 Lys Arg Trp Ile Ile Leu Gly Leu Asn Lys Ile Val Arg Met Tyr Ser 370 375 380 Pro Thr Ser Ile Leu Asp Ile Arg Gln Gly Pro Lys Glu Pro Phe Arg 385 390 395 400 Asp Tyr Val Asp Arg Phe Tyr Lys Thr Leu Arg Ala Glu Gln Ala Ser 405 410 415 Gln Glu Val Lys Asn Trp Met Thr Glu Thr Leu Leu Val Gln Asn Ala 420 425 430 Asn Pro Asp Cys Lys Thr Ile Leu Lys Ala Leu Gly Pro Ala Ala Thr 435 440 445 Leu Glu Glu Met Met Thr Ala Cys Gln Gly Val Gly Gly Pro Gly His 450 455 460 Lys Ala Arg Val Leu Glu Phe 465 470 2 380 DNA Artificial Sequence Description of Artificial SequenceCyclin B sequence used in constructs 2 atgtccagtg atttggagaa tattgacaca ggagttaatt ctaaagttaa gagtcatgtg 60 actattaggc gaactgtttt agaagaaatt ggaaatagag ttacaaccag agcagcacaa 120 gtagctaaga aagctcagaa caccaaagtt ccagttcaac ccaccaaaac aacaaatgtc 180 aacaaacaac tgaaacctac tgcttctgtc aaaccagtac agatggaaaa gttggctcca 240 aagggtcctt ctcccacacc tgtcgacaga gagatgggtg cgagagcgtc agtattaagc 300 gggggagaat tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa gaagaagtac 360 aagctaaagc acatcgtatg 380 3 91 PRT Artificial Sequence Description of Artificial SequenceCyclin B sequence used in constructs 3 Met Ser Ser Asp Leu Glu Asn Ile Asp Thr Gly Val Asn Ser Lys Val 1 5 10 15 Lys Ser His Val Thr Ile Arg Arg Thr Val Leu Glu Glu Ile Gly Asn 20 25 30 Arg Val Thr Thr Arg Ala Ala Gln Val Ala Lys Lys Ala Gln Asn Thr 35 40 45 Lys Val Pro Val Gln Pro Thr Lys Thr Thr Asn Val Asn Lys Gln Leu 50 55 60 Lys Pro Thr Ala Ser Val Lys Pro Val Gln Met Glu Lys Leu Ala Pro 65 70 75 80 Lys Gly Pro Ser Pro Thr Pro Val Asp Arg Glu 85 90 4 27 DNA Artificial Sequence Description of Artificial Sequence c-Mos sequence used in constructs 4 atgcccgatc ccctggtcga cagagag 27 5 9 PRT Artificial Sequence Description of Artificial Sequence c-Mos sequence used in constructs 5 Met Pro Asp Pro Leu Val Asp Arg Glu 1 5 6 30 PRT Artificial Sequence Description of Artificial Sequencebeta-catenin (18-47) 6 Arg Lys Ala Ala Val Ser His Trp Gln Gln Gln Ser Tyr Leu Asp Ser 1 5 10 15 Gly Ile His Ser Gly Ala Thr Thr Thr Ala Pro Ser Leu Ser 20 25 30 7 31 PRT Artificial Sequence Description of Artificial Sequencebeta-catenin (18-47) with initiator Met 7 Met Arg Lys Ala Ala Val Ser His Trp Gln Gln Gln Ser Tyr Leu Asp 1 5 10 15 Ser Gly Ile His Ser Gly Ala Thr Thr Thr Ala Pro Ser Leu Ser 20 25 30 8 6978 DNA Artificial Sequence Description of Artificial Sequencevector pCMVkan/R-R-SIVgp160CTE containing mutated SIV env gene 8 cctggccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc 60 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg 120 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 180 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 240 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 300 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 360 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 420 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 480 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 540 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 600 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 660 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata 720 gaagacaccg ggaccgatcc agcctccgcg ggccgcgcta agtatgggat gtcttgggaa 780 tcagctgctt atcgccatct tgcttttaag tgtctatggg atctattgta ctctatatgt 840 cacagtcttt tatggtgtac cagcttggag gaatgcgaca attcccctct tttgtgcaac 900 caagaatagg gatacttggg gaacaactca gtgcctacca gataatggtg attattcaga 960 agtggccctt aatgttacag aaagctttga tgcctggaat aatacagtca cagaacaggc 1020 aatagaggat gtatggcaac tctttgagac ctcaataaag ccttgtgtaa aattatcccc 1080 attatgcatt actatgagat gcaataaaag tgagacagat agatggggat tgacaaaatc 1140 aataacaaca acagcatcaa caacatcaac gacagcatca gcaaaagtag acatggtcaa 1200 tgagactagt tcttgtatag cccaggataa ttgcacaggc ttggaacaag agcaaatgat 1260 aagctgtaaa ttcaacatga cagggttaaa aagagacaag aaaaaagagt acaatgaaac 1320 ttggtactct gcagatttgg tatgtgaaca agggaataac actggtaatg aaagtagatg 1380 ttacatgaac cactgtaaca cttctgttat ccaagagtct tgtgacaaac attattggga 1440 tgctattaga tttaggtatt gtgcacctcc aggttatgct ttgcttagat gtaatgacac 1500 aaattattca ggctttatgc ctaaatgttc taaggtggtg gtctcttcat gcacaaggat 1560 gatggagaca cagacttcta cttggtttgg ctttaatgga actagagcag aaaatagaac 1620 ttatatttac tggcatggta gggataatag gactataatt agtttaaata agtattataa 1680 tctaacaatg aaatgtagaa gaccaggaaa taagacagtt ttaccagtca ccattatgtc 1740 tggattggtt ttccactcac aaccaatcaa tgataggcca aagcaggcat ggtgttggtt 1800 tggaggaaaa tggaaggatg caataaaaga ggtgaagcag accattgtca aacatcccag 1860 gtatactgga actaacaata ctgataaaat caatttgacg gctcctggag gaggagatcc 1920 ggaagttacc ttcatgtgga caaattgcag aggagagttc ctctactgta aaatgaattg 1980 gtttctaaat tgggtagaag ataggaatac agctaaccag aagccaaagg aacagcataa 2040 aaggaattac gtgccatgtc atattagaca aataatcaac acttggcata aagtaggcaa 2100 aaatgtttat ttgcctccaa gagagggaga cctcacgtgt aactccacag tgaccagtct 2160 catagcaaac atagattgga ttgatggaaa ccaaactaat atcaccatga gtgcagaggt 2220 ggcagaactg tatcgattgg aattgggaga ttataaatta gtagagatca ctccaattgg 2280 cttggccccc acagatgtga agaggtacac tactggtggc acctcaagaa ataaaagagg 2340 ggtctttgtg ctagggttct tgggttttct cgcaacggca ggttctgcaa tgggagccgc 2400 cagcctgacc ctcacggcac agtcccgaac tttattggct gggatagtcc aacagcagca 2460 acagctgttg gacgtggtca agagacaaca agaattgttg cgactgaccg tctggggaac 2520 aaagaacctc cagactaggg tcactgccat cgagaagtac ttaaaggacc aggcgcagct 2580 gaatgcttgg ggatgtgcgt ttagacaagt ctgccacact actgtaccat ggccaaatgc 2640 aagtctaaca ccaaagtgga acaatgagac ttggcaagag tgggagcgaa aggttgactt 2700 cttggaagaa aatataacag ccctcctaga ggaggcacaa attcaacaag agaagaacat 2760 gtatgaatta caaaagttga atagctggga tgtgtttggc aattggtttg accttgcttc 2820 ttggataaag tatatacaat atggagttta tatagttgta ggagtaatac tgttaagaat 2880 agtgatctat atagtacaaa tgctagctaa gttaaggcag gggtataggc cagtgttctc 2940 ttccccaccc tcttatttcc agcagaccca tatccaacag gacccggcac tgccaaccag 3000 agaaggcaaa gaaagagacg gtggagaagg cggtggcaac agctcctggc cttggcagat 3060 agaatatatc cactttctta ttcgtcagct tattagactc ttgacttggc tattcagtaa 3120 ctgtaggact ttgctatcga gagtatacca gatcctccaa ccaatactcc agaggctctc 3180 tgcgacccta cagaggattc gagaagtcct caggactgaa ctgacctacc tacaatatgg 3240 gtggagctat ttccatgagg cggtccaggc cgtctggaga tctgcgacag agactcttgc 3300 gggcgcgtgg ggagacttat gggagactct taggagaggt ggaagatgga tactcgcaat 3360 ccccaggagg attagacaag ggcttgagct cactctcttg tgagggacag agaattcgga 3420 tccactagtt ctagactcga gggggggccc ggtacgagcg cttagctagc tagagaccac 3480 ctcccctgcg agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa 3540 cctaagacag gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa 3600 aatgtatcac tccaacctaa gacaggcgca gcttccgagg gatttgtcgt ctgttttata 3660 tatatttaaa agggtgacct gtccggagcc gtgctgcccg gatgatgtct tggtctagac 3720 tcgagggggg gcccggtacg atccagatct gctgtgcctt ctagttgcca gccatctgtt 3780 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 3840 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 3900 ggggtggggc agcacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 3960 gcggtgggct ctatgggtac ccaggtgctg aagaattgac ccggttcctc ctgggccaga 4020 aagaagcagg cacatcccct tctctgtgac acaccctgtc cacgcccctg gttcttagtt 4080 ccagccccac tcataggaca ctcatagctc aggagggctc cgccttcaat cccacccgct 4140 aaagtacttg gagcggtctc tccctccctc atcagcccac caaaccaaac ctagcctcca 4200 agagtgggaa gaaattaaag caagataggc tattaagtgc agagggagag aaaatgcctc 4260 caacatgtga ggaagtaatg agagaaatca tagaatttct tccgcttcct cgctcactga 4320 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4380 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4440 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4500 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4560 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4620 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 4680 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4740 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4800 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4860 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4920 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4980 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 5040 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 5100 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 5160 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 5220 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 5280 tctatttcgt tcatccatag ttgcctgact ccgggggggg ggggcgctga ggtctgcctc 5340 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 5400 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 5460 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 5520 cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg 5580 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa 5640 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa 5700 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc 5760 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt 5820 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg 5880 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc 5940 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct 6000 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc 6060 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc 6120 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt 6180 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt 6240 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa 6300 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa 6360 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg 6420 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga 6480 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc 6540 ccccccccat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6600 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6660 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 6720 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 6780 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 6840 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 6900 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 6960 catcagattg gctattgg 6978 9 879 PRT Artificial Sequence Description of Artificial Sequenceprotein encoded by nucleotide positions 764-3400 of vector pCMVkan/R-R-SIVgp160CTE containing mutated SIV env gene 9 Met Gly Cys Leu Gly Asn Gln Leu Leu Ile Ala Ile Leu Leu Leu Ser 1 5 10 15 Val Tyr Gly Ile Tyr Cys Thr Leu Tyr Val Thr Val Phe Tyr Gly Val 20 25 30 Pro Ala Trp Arg Asn Ala Thr Ile Pro Leu Phe Cys Ala Thr Lys Asn 35 40 45 Arg Asp Thr Trp Gly Thr Thr Gln Cys Leu Pro Asp Asn Gly Asp Tyr 50 55 60 Ser Glu Val Ala Leu Asn Val Thr Glu Ser Phe Asp Ala Trp Asn Asn 65 70 75 80 Thr Val Thr Glu Gln Ala Ile Glu Asp Val Trp Gln Leu Phe Glu Thr 85 90 95 Ser Ile Lys Pro Cys Val Lys Leu Ser Pro Leu Cys Ile Thr Met Arg 100 105 110 Cys Asn Lys Ser Glu Thr Asp Arg Trp Gly Leu Thr Lys Ser Ile Thr 115 120 125 Thr Thr Ala Ser Thr Thr Ser Thr Thr Ala Ser Ala Lys Val Asp Met 130 135 140 Val Asn Glu Thr Ser Ser Cys Ile Ala Gln Asp Asn Cys Thr Gly Leu 145 150 155 160 Glu Gln Glu Gln Met Ile Ser Cys Lys Phe Asn Met Thr Gly Leu Lys 165 170 175 Arg Asp Lys Lys Lys Glu Tyr Asn Glu Thr Trp Tyr Ser Ala Asp Leu 180 185 190 Val Cys Glu Gln Gly Asn Asn Thr Gly Asn Glu Ser Arg Cys Tyr Met 195 200 205 Asn His Cys Asn Thr Ser Val Ile Gln Glu Ser Cys Asp Lys His Tyr 210 215 220 Trp Asp Ala Ile Arg Phe Arg Tyr Cys Ala Pro Pro Gly Tyr Ala Leu 225 230 235 240 Leu Arg Cys Asn Asp Thr Asn Tyr Ser Gly Phe Met Pro Lys Cys Ser 245 250 255 Lys Val Val Val Ser Ser Cys Thr Arg Met Met Glu Thr Gln Thr Ser 260 265 270 Thr Trp Phe Gly Phe Asn Gly Thr Arg Ala Glu Asn Arg Thr Tyr Ile 275 280 285 Tyr Trp His Gly Arg Asp Asn Arg Thr Ile Ile Ser Leu Asn Lys Tyr 290 295 300 Tyr Asn Leu Thr Met Lys Cys Arg Arg Pro Gly Asn Lys Thr Val Leu 305 310 315 320 Pro Val Thr Ile Met Ser Gly Leu Val Phe His Ser Gln Pro Ile Asn 325 330 335 Asp Arg Pro Lys Gln Ala Trp Cys Trp Phe Gly Gly Lys Trp Lys Asp 340 345 350 Ala Ile Lys Glu Val Lys Gln Thr Ile Val Lys His Pro Arg Tyr Thr 355 360 365 Gly Thr Asn Asn Thr Asp Lys Ile Asn Leu Thr Ala Pro Gly Gly Gly 370 375 380 Asp Pro Glu Val Thr Phe Met Trp Thr Asn Cys Arg Gly Glu Phe Leu 385 390 395 400 Tyr Cys Lys Met Asn Trp Phe Leu Asn Trp Val Glu Asp Arg Asn Thr 405 410 415 Ala Asn Gln Lys Pro Lys Glu Gln His Lys Arg Asn Tyr Val Pro Cys 420 425 430 His Ile Arg Gln Ile Ile Asn Thr Trp His Lys Val Gly Lys Asn Val 435 440 445 Tyr Leu Pro Pro Arg Glu Gly Asp Leu Thr Cys Asn Ser Thr Val Thr 450 455 460 Ser Leu Ile Ala Asn Ile Asp Trp Ile Asp Gly Asn Gln Thr Asn Ile 465 470 475 480 Thr Met Ser Ala Glu Val Ala Glu Leu Tyr Arg Leu Glu Leu Gly Asp 485 490 495 Tyr Lys Leu Val Glu Ile Thr Pro Ile Gly Leu Ala Pro Thr Asp Val 500 505 510 Lys Arg Tyr Thr Thr Gly Gly Thr Ser Arg Asn Lys Arg Gly Val Phe 515 520 525 Val Leu Gly Phe Leu Gly Phe Leu Ala Thr Ala Gly Ser Ala Met Gly 530 535 540 Ala Ala Ser Leu Thr Leu Thr Ala Gln Ser Arg Thr Leu Leu Ala Gly 545 550 555 560 Ile Val Gln Gln Gln Gln Gln Leu Leu Asp Val Val Lys Arg Gln Gln 565 570 575 Glu Leu Leu Arg Leu Thr Val Trp Gly Thr Lys Asn Leu Gln Thr Arg 580 585 590 Val Thr Ala Ile Glu Lys Tyr Leu Lys Asp Gln Ala Gln Leu Asn Ala 595 600 605 Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Pro 610 615 620 Asn Ala Ser Leu Thr Pro Lys Trp Asn Asn Glu Thr Trp Gln Glu Trp 625 630 635 640 Glu Arg Lys Val Asp Phe Leu Glu Glu Asn Ile Thr Ala Leu Leu Glu 645 650 655 Glu Ala Gln Ile Gln Gln Glu Lys Asn Met Tyr Glu Leu Gln Lys Leu 660 665 670 Asn Ser Trp Asp Val Phe Gly Asn Trp Phe Asp Leu Ala Ser Trp Ile 675 680 685 Lys Tyr Ile Gln Tyr Gly Val Tyr Ile Val Val Gly Val Ile Leu Leu 690 695 700 Arg Ile Val Ile Tyr Ile Val Gln Met Leu Ala Lys Leu Arg Gln Gly 705 710 715 720 Tyr Arg Pro Val Phe Ser Ser Pro Pro Ser Tyr Phe Gln Gln Thr His 725 730 735 Ile Gln Gln Asp Pro Ala Leu Pro Thr Arg Glu Gly Lys Glu Arg Asp 740 745 750 Gly Gly Glu Gly Gly Gly Asn Ser Ser Trp Pro Trp Gln Ile Glu Tyr 755 760 765 Ile His Phe Leu Ile Arg Gln Leu Ile Arg Leu Leu Thr Trp Leu Phe 770 775 780 Ser Asn Cys Arg Thr Leu Leu Ser Arg Val Tyr Gln Ile Leu Gln Pro 785 790 795 800 Ile Leu Gln Arg Leu Ser Ala Thr Leu Gln Arg Ile Arg Glu Val Leu 805 810 815 Arg Thr Glu Leu Thr Tyr Leu Gln Tyr Gly Trp Ser Tyr Phe His Glu 820 825 830 Ala Val Gln Ala Val Trp Arg Ser Ala Thr Glu Thr Leu Ala Gly Ala 835 840 845 Trp Gly Asp Leu Trp Glu Thr Leu Arg Arg Gly Gly Arg Trp Ile Leu 850 855 860 Ala Ile Pro Arg Arg Ile Arg Gln Gly Leu Glu Leu Thr Leu Leu 865 870 875 10 271 PRT Artificial Sequence Description of Artificial Sequenceprotein encoded by the complement of nucleotide positions 6426-5614 of vector pCMVkan/R-R-SIVgp160CTE containing mutated SIV env gene 10 Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 1 5 10 15 Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 20 25 30 Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 35 40 45 Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 50 55 60 Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 65 70 75 80 Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 85 90 95 Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 100 105 110 Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 115 120 125 Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 130 135 140 Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 145 150 155 160 Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 165 170 175 Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 180 185 190 Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 195 200 205 Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 210 215 220 Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 225 230 235 240 Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 245 250 255 Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 260 265 270 11 2796 DNA Artificial Sequence Description of Artificial SequenceMCP3-gp160 env (HIV) fusion 11 atgaacccaa gtgctgccgt cattttctgc ctcatcctgc tgggtctgag tgggactcaa 60 gggatcctcg acatggcgca accggtaggt ataaacacaa gcacaacctg ttgctatcgt 120 ttcataaata aaaagatacc gaagcaacgt ctggaaagct atcgccgtac cacttctagc 180 cactgtccgc gtgaagctgt tatattcaaa acgaaactgg ataaggagat ctgcgccgac 240 cctacacaga aatgggttca ggactttatg aagcacctgg ataaaaagac acagacgccg 300 aaactgatct gcagcgccga ggagaagctg tgggtcacgg tctattatgg cgtgcccgtg 360 tggaaagagg caaccaccac gctattctgc gcctccgacg ccaaggcaca tcatgcagag 420 gcgcacaacg tctgggccac gcatgcctgt gtacccacgg accctaaccc ccaagaggtg 480 atcctggaga acgtgaccga gaagtacaac atgtggaaaa ataacatggt agaccagatg 540 catgaggata taatcagtct atgggatcaa agcctaaagc catgtgtaaa actaaccccc 600 ctctgcgtga cgctgaattg caccaacgcg acgtatacga atagtgacag taagaatagt 660 accagtaata gtagtttgga ggacagtggg aaaggagaca tgaactgctc gttcgatgtc 720 accaccagca tcgacaagaa gaagaagacg gagtatgcca tcttcgacaa gctggatgta 780 atgaatatag gaaatggaag atatacgcta ttgaattgta acaccagtgt cattacgcag 840 gcctgtccaa agatgtcctt tgagccaatt cccatacatt attgtacccc ggccggctac 900 gcgatcctga agtgcaacga caataagttc aatggaacgg gaccatgtac gaatgtcagc 960 acgatacaat gtacgcatgg aattaagcca gtagtgtcga cgcaactgct gctgaacggc 1020 agcctggccg agggaggaga ggtaataatt cggtcggaga acctcaccga caacgccaag 1080 accataatag tacagctcaa ggaacccgtg gagatcaact gtacgagacc caacaacaac 1140 acccgaaaga gcatacatat gggaccagga gcagcatttt atgcaagagg agaggtaata 1200 ggagatataa gacaagcaca ttgcaacatt agtagaggaa gatggaatga cactttgaaa 1260 cagatagcta aaaagctgcg cgagcagttt aacaagacca taagccttaa ccaatcctcg 1320 ggaggggacc tagagattgt aatgcacacg tttaattgtg gaggggagtt tttctactgt 1380 aacacgaccc agctgttcaa cagcacctgg aatgagaatg atacgacctg gaataatacg 1440 gcagggtcga ataacaatga gacgatcacc ctgccctgtc gcatcaagca gatcataaac 1500 aggtggcagg aagtaggaaa agcaatgtat gcccctccca tcagtggccc gatcaactgc 1560 ttgtccaaca tcaccgggct attgttgacg agagatggtg gtgacaacaa taatacgata 1620 gagaccttca gacctggagg aggagatatg agggacaact ggaggagcga gctgtacaag 1680 tacaaggtag tgaggatcga gccattggga atagcaccca ccaaggcaaa gagaagagtg 1740 gtgcaaagag agaaaagagc agtgggaata ggagctatgt tccttgggtt cttgggagca 1800 gcaggaagca ctatgggcgc agcgtcggtg acccttaccg tgcaagctcg cctgctgctg 1860 tcgggtatag tgcaacagca aaacaacctc ctccgcgcaa tcgaagccca gcagcatctg 1920 ttgcaactca cggtctgggg catcaagcag ctccaggcta gagtccttgc catggagcgt 1980 tatctgaaag accagcaact tcttgggatt tggggttgct cgggaaaact catttgcacc 2040 acgaatgtgc cttggaacgc cagctggagc aacaagtccc tggacaagat ttggcataac 2100 atgacctgga tggagtggga ccgcgagatc gacaactaca cgaaattgat atacaccctg 2160 atcgaggcgt cccagatcca gcaggagaag aatgagcaag agttgttgga gttggattcg 2220 tgggcgtcgt tgtggtcgtg gtttgacatc tcgaaatggc tgtggtatat aggagtattc 2280 ataatagtaa taggaggttt ggtaggtttg aaaatagttt ttgctgtact ttcgatagta 2340 aatcgagtta ggcagggata ctcgccattg tcatttcaaa cccgcctccc agccccgcgg 2400 ggacccgaca ggcccgaggg catcgaggag ggaggcggcg agagagacag agacagatcc 2460 gatcaattgg tgacgggatt cttggcactc atctgggacg atctgcggag cctgtgcctc 2520 ttctcttacc accgcctgcg cgacctgctc ctgatcgtgg cgaggatcgt ggagcttctg 2580 ggacgcaggg ggtgggaggc cctgaagtac tggtggaacc tcctgcaata ttggattcag 2640 gagctgaaga acagcgccgt tagtctgctg aacgctaccg ctatcgccgt ggcggaagga 2700 accgacagga ttatagaggt agtacaaagg attggtcgcg ccatcctcca tatcccccgc 2760 cgcatccgcc agggcttgga gagggctttg ctataa 2796 12 931 PRT Artificial Sequence Description of Artificial Sequence MCP3-gp160 env (HIV) fusion 12 Met Asn Pro Ser Ala Ala Val Ile Phe Cys Leu Ile Leu Leu Gly Leu 1 5 10 15 Ser Gly Thr Gln Gly Ile Leu Asp Met Ala Gln Pro Val Gly Ile Asn 20 25 30 Thr Ser Thr Thr Cys Cys Tyr Arg Phe Ile Asn Lys Lys Ile Pro Lys 35 40 45 Gln Arg Leu Glu Ser Tyr Arg Arg Thr Thr Ser Ser His Cys Pro Arg 50 55 60 Glu Ala Val Ile Phe Lys Thr Lys Leu Asp Lys Glu Ile Cys Ala Asp 65 70 75 80 Pro Thr Gln Lys Trp Val Gln Asp Phe Met Lys His Leu Asp Lys Lys 85 90 95 Thr Gln Thr Pro Lys Leu Ile Cys Ser Ala Glu Glu Lys Leu Trp Val 100 105 110 Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr Thr Thr Leu 115 120 125 Phe Cys Ala Ser Asp Ala Lys Ala His His Ala Glu Ala His Asn Val 130 135 140 Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln Glu Val 145 150 155 160 Ile Leu Glu Asn Val Thr Glu Lys Tyr Asn Met Trp Lys Asn Asn Met 165 170 175 Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln Ser Leu 180 185 190 Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn Cys Thr 195 200 205 Asn Ala Thr Tyr Thr Asn Ser Asp Ser Lys Asn Ser Thr Ser Asn Ser 210 215 220 Ser Leu Glu Asp Ser Gly Lys Gly Asp Met Asn Cys Ser Phe Asp Val 225 230 235 240 Thr Thr Ser Ile Asp Lys Lys Lys Lys Thr Glu Tyr Ala Ile Phe Asp 245 250 255 Lys Leu Asp Val Met Asn Ile Gly Asn Gly Arg Tyr Thr Leu Leu Asn 260 265 270 Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Met Ser Phe Glu 275 280 285 Pro Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Tyr Ala Ile Leu Lys 290 295 300 Cys Asn Asp Asn Lys Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser 305 310 315 320 Thr Ile Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu 325 330 335 Leu Leu Asn Gly Ser Leu Ala Glu Gly Gly Glu Val Ile Ile Arg Ser 340 345 350 Glu Asn Leu Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Lys Glu 355 360 365 Pro Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 370 375 380 Ile His Met Gly Pro Gly Ala Ala Phe Tyr Ala Arg Gly Glu Val Ile 385 390 395 400 Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Gly Arg Trp Asn 405 410 415 Asp Thr Leu Lys Gln Ile Ala Lys Lys Leu Arg Glu Gln Phe Asn Lys 420 425 430 Thr Ile Ser Leu Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Val Met 435 440 445 His Thr Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Thr Gln 450 455 460 Leu Phe Asn Ser Thr Trp Asn Glu Asn Asp Thr Thr Trp Asn Asn Thr 465 470 475 480 Ala Gly Ser Asn Asn Asn Glu Thr Ile Thr Leu Pro Cys Arg Ile Lys 485 490 495 Gln Ile Ile Asn Arg Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro 500 505 510 Pro Ile Ser Gly Pro Ile Asn Cys Leu Ser Asn Ile Thr Gly Leu Leu 515 520 525 Leu Thr Arg Asp Gly Gly Asp Asn Asn Asn Thr Ile Glu Thr Phe Arg 530 535 540 Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys 545 550 555 560 Tyr Lys Val Val Arg Ile Glu Pro Leu Gly Ile Ala Pro Thr Lys Ala 565 570 575 Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala Val Gly Ile Gly Ala 580 585 590 Met Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala 595 600 605 Ser Val Thr Leu Thr Val Gln Ala Arg Leu Leu Leu Ser Gly Ile Val 610 615 620 Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu 625 630 635 640 Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 645 650 655 Ala Met Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly 660 665 670 Cys Ser Gly Lys Leu Ile Cys Thr Thr Asn Val Pro Trp Asn Ala Ser 675 680 685 Trp Ser Asn Lys Ser Leu Asp Lys Ile Trp His Asn Met Thr Trp Met 690 695 700 Glu Trp Asp Arg Glu Ile Asp Asn Tyr Thr Lys Leu Ile Tyr Thr Leu 705 710 715 720 Ile Glu Ala Ser Gln Ile Gln Gln Glu Lys Asn Glu Gln Glu Leu Leu 725 730 735 Glu Leu Asp Ser Trp Ala Ser Leu Trp Ser Trp Phe Asp Ile Ser Lys 740 745 750 Trp Leu Trp Tyr Ile Gly Val Phe Ile Ile Val Ile Gly Gly Leu Val 755 760 765 Gly Leu Lys Ile Val Phe Ala Val Leu Ser Ile Val Asn Arg Val Arg 770 775 780 Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr Arg Leu Pro Ala Pro Arg 785 790 795 800 Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Gly Gly Gly Glu Arg Asp 805 810 815 Arg Asp Arg Ser Asp Gln Leu Val Thr Gly Phe Leu Ala Leu Ile Trp 820 825 830 Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr His Arg Leu Arg Asp 835 840 845 Leu Leu Leu Ile Val Ala Arg Ile Val Glu Leu Leu Gly Arg Arg Gly 850 855 860 Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr Trp Ile Gln 865 870 875 880 Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn Ala Thr Ala Ile Ala 885 890 895 Val Ala Glu Gly Thr Asp Arg Ile Ile Glu Val Val Gln Arg Ile Gly 900 905 910 Arg Ala Ile Leu His Ile Pro Arg Arg Ile Arg Gln Gly Leu Glu Arg 915 920 925 Ala Leu Leu 930 13 2583 DNA Artificial Sequence Description of Artificial Sequencebeta-catenin-gp160 env (HIV) fusion 13 atgagaaaag cggctgttag tcactggcag cagcagtctt acctggactc tggaatccat 60 tctggtgcca ctaccacagc tccttctctg agtatctgca gcgccgagga gaagctgtgg 120 gtcacggtct attatggcgt gcccgtgtgg aaagaggcaa ccaccacgct attctgcgcc 180 tccgacgcca aggcacatca tgcagaggcg cacaacgtct gggccacgca tgcctgtgta 240 cccacggacc ctaaccccca agaggtgatc ctggagaacg tgaccgagaa gtacaacatg 300 tggaaaaata acatggtaga ccagatgcat gaggatataa tcagtctatg ggatcaaagc 360 ctaaagccat gtgtaaaact aacccccctc tgcgtgacgc tgaattgcac caacgcgacg 420 tatacgaata gtgacagtaa gaatagtacc agtaatagta gtttggagga cagtgggaaa 480 ggagacatga actgctcgtt cgatgtcacc accagcatcg acaagaagaa gaagacggag 540 tatgccatct tcgacaagct ggatgtaatg aatataggaa atggaagata tacgctattg 600 aattgtaaca ccagtgtcat tacgcaggcc tgtccaaaga tgtcctttga gccaattccc 660 atacattatt gtaccccggc cggctacgcg atcctgaagt gcaacgacaa taagttcaat 720 ggaacgggac catgtacgaa tgtcagcacg atacaatgta cgcatggaat taagccagta 780 gtgtcgacgc aactgctgct gaacggcagc ctggccgagg gaggagaggt aataattcgg 840 tcggagaacc tcaccgacaa cgccaagacc ataatagtac agctcaagga acccgtggag 900 atcaactgta cgagacccaa caacaacacc cgaaagagca tacatatggg accaggagca 960 gcattttatg caagaggaga ggtaatagga gatataagac aagcacattg caacattagt 1020 agaggaagat ggaatgacac tttgaaacag atagctaaaa agctgcgcga gcagtttaac 1080 aagaccataa gccttaacca atcctcggga ggggacctag agattgtaat gcacacgttt 1140 aattgtggag gggagttttt ctactgtaac acgacccagc tgttcaacag cacctggaat 1200 gagaatgata cgacctggaa taatacggca gggtcgaata acaatgagac gatcaccctg 1260 ccctgtcgca tcaagcagat cataaacagg tggcaggaag taggaaaagc aatgtatgcc 1320 cctcccatca gtggcccgat caactgcttg tccaacatca ccgggctatt gttgacgaga 1380 gatggtggtg acaacaataa tacgatagag accttcagac ctggaggagg agatatgagg 1440 gacaactgga ggagcgagct gtacaagtac aaggtagtga ggatcgagcc attgggaata 1500 gcacccacca aggcaaagag aagagtggtg caaagagaga aaagagcagt gggaatagga 1560 gctatgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcggtgacc 1620 cttaccgtgc aagctcgcct gctgctgtcg ggtatagtgc aacagcaaaa caacctcctc 1680 cgcgcaatcg aagcccagca gcatctgttg caactcacgg tctggggcat caagcagctc 1740 caggctagag tccttgccat ggagcgttat ctgaaagacc agcaacttct tgggatttgg 1800 ggttgctcgg gaaaactcat ttgcaccacg aatgtgcctt ggaacgccag ctggagcaac 1860 aagtccctgg acaagatttg gcataacatg acctggatgg agtgggaccg cgagatcgac 1920 aactacacga aattgatata caccctgatc gaggcgtccc agatccagca ggagaagaat 1980 gagcaagagt tgttggagtt ggattcgtgg gcgtcgttgt ggtcgtggtt tgacatctcg 2040 aaatggctgt ggtatatagg agtattcata atagtaatag gaggtttggt aggtttgaaa 2100 atagtttttg ctgtactttc gatagtaaat cgagttaggc agggatactc gccattgtca 2160 tttcaaaccc gcctcccagc cccgcgggga cccgacaggc ccgagggcat cgaggaggga 2220 ggcggcgaga gagacagaga cagatccgat caattggtga cgggattctt ggcactcatc 2280 tgggacgatc tgcggagcct gtgcctcttc tcttaccacc gcctgcgcga cctgctcctg 2340 atcgtggcga ggatcgtgga gcttctggga cgcagggggt gggaggccct gaagtactgg 2400 tggaacctcc tgcaatattg gattcaggag ctgaagaaca gcgccgttag tctgctgaac 2460 gctaccgcta tcgccgtggc ggaaggaacc gacaggatta tagaggtagt acaaaggatt 2520 ggtcgcgcca tcctccatat cccccgccgc atccgccagg gcttggagag ggctttgcta 2580 taa 2583 14 860 PRT Artificial Sequence Description of Artificial Sequencebeta-catenin-gp160 env (HIV) fusion 14 Met Arg Lys Ala Ala Val Ser His Trp Gln Gln Gln Ser Tyr Leu Asp 1 5 10 15 Ser Gly Ile His Ser Gly Ala Thr Thr Thr Ala Pro Ser Leu Ser Ile 20 25 30 Cys Ser Ala Glu Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro 35 40 45 Val Trp Lys Glu Ala Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys 50 55 60 Ala His His Ala Glu Ala His Asn Val Trp Ala Thr His Ala Cys Val 65 70 75 80 Pro Thr Asp Pro Asn Pro Gln Glu Val Ile Leu Glu Asn Val Thr Glu 85 90 95 Lys Tyr Asn Met Trp Lys Asn Asn Met Val Asp Gln Met His Glu Asp 100 105 110 Ile Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr 115 120 125 Pro Leu Cys Val Thr Leu Asn Cys Thr Asn Ala Thr Tyr Thr Asn Ser 130 135 140 Asp Ser Lys Asn Ser Thr Ser Asn Ser Ser Leu Glu Asp Ser Gly Lys 145 150 155 160 Gly Asp Met Asn Cys Ser Phe Asp Val Thr Thr Ser Ile Asp Lys Lys 165 170 175 Lys Lys Thr Glu Tyr Ala Ile Phe Asp Lys Leu Asp Val Met Asn Ile 180 185 190 Gly Asn Gly Arg Tyr Thr Leu Leu Asn Cys Asn Thr Ser Val Ile Thr 195 200 205 Gln Ala Cys Pro Lys Met Ser Phe Glu Pro Ile Pro Ile His Tyr Cys 210 215 220 Thr Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asp Asn Lys Phe Asn 225 230 235 240 Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Ile Gln Cys Thr His Gly 245 250 255 Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 260 265 270 Glu Gly Gly Glu Val Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn Ala 275 280 285 Lys Thr Ile Ile Val Gln Leu Lys Glu Pro Val Glu Ile Asn Cys Thr 290 295 300 Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile His Met Gly Pro Gly Ala 305 310 315 320 Ala Phe Tyr Ala Arg Gly Glu Val Ile Gly Asp Ile Arg Gln Ala His 325 330 335 Cys Asn Ile Ser Arg Gly Arg Trp Asn Asp Thr Leu Lys Gln Ile Ala 340 345 350 Lys Lys Leu Arg Glu Gln Phe Asn Lys Thr Ile Ser Leu Asn Gln Ser 355 360 365 Ser Gly Gly Asp Leu Glu Ile Val Met His Thr Phe Asn Cys Gly Gly 370 375 380 Glu Phe Phe Tyr Cys Asn Thr Thr Gln Leu Phe Asn Ser Thr Trp Asn 385 390 395 400 Glu Asn Asp Thr Thr Trp Asn Asn Thr Ala Gly Ser Asn Asn Asn Glu 405 410 415 Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Arg Trp Gln 420 425 430 Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Pro Ile Asn 435 440 445 Cys Leu Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asp 450 455 460 Asn Asn Asn Thr Ile Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg 465 470 475 480 Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu 485 490 495 Pro Leu Gly Ile Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg 500 505 510 Glu Lys Arg Ala Val Gly Ile Gly Ala Met Phe Leu Gly Phe Leu Gly 515 520 525 Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Val Thr Leu Thr Val Gln 530 535 540 Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu 545 550 555 560 Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly 565 570 575 Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Met Glu Arg Tyr Leu Lys 580 585 590 Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 595 600 605 Thr Thr Asn Val Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Asp 610 615 620 Lys Ile Trp His Asn Met Thr Trp Met Glu Trp Asp Arg Glu Ile Asp 625 630 635 640 Asn Tyr Thr Lys Leu Ile Tyr Thr Leu Ile Glu Ala Ser Gln Ile Gln 645 650 655 Gln Glu Lys Asn Glu Gln Glu Leu Leu Glu Leu Asp Ser Trp Ala Ser 660 665 670 Leu Trp Ser Trp Phe Asp Ile Ser Lys Trp Leu Trp Tyr Ile Gly Val 675 680 685 Phe Ile Ile Val Ile Gly Gly Leu Val Gly Leu Lys Ile Val Phe Ala 690 695 700 Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 705 710 715 720 Phe Gln Thr Arg Leu Pro Ala Pro Arg Gly Pro Asp Arg Pro Glu Gly 725 730 735 Ile Glu Glu Gly Gly Gly Glu Arg Asp Arg Asp Arg Ser Asp Gln Leu 740 745 750 Val Thr Gly Phe Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys 755 760 765 Leu Phe Ser Tyr His Arg Leu Arg Asp Leu Leu Leu Ile Val Ala Arg 770 775 780 Ile Val Glu Leu Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp 785 790 795 800 Trp Asn Leu Leu Gln Tyr Trp Ile Gln Glu Leu Lys Asn Ser Ala Val 805 810 815 Ser Leu Leu Asn Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg 820 825 830 Ile Ile Glu Val Val Gln Arg Ile Gly Arg Ala Ile Leu His Ile Pro 835 840 845 Arg Arg Ile Arg Gln Gly Leu Glu Arg Ala Leu Leu 850 855 860

Claims

What is claimed is:

1. A nucleic acid construct containing nucleotide sequences encoding a fusion protein comprising a destabilizing amino acid sequence covalently attached to a heterologous amino acid sequence of interest in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence and wherein the destabilizing amino acid sequence is present in the amino acid sequences selected from the group consisting of c-Myc aa2-120; Cyclin A aa13-91; Cyclin B 10-95; Cyclin B aa13-91; IkBa aa20-45; β-Catenin aa19-44; c-Jun aa1-67; and c-Mos aa1-35.

2. A nucleic acid construct of claim 1 wherein the amino acid sequence of interest is a disease associated antigen.

3. A nucleic acid construct of claim 1 wherein the destabilization sequence A nucleic acid construct of claim 1 wherein the destabilization sequence is selected from the group consisting of c-Mos aa1-35; cyclin B aa 10-95; β-catenin 19-44 and β-catenin 18-47.

4. The nucleic acid construct of claim 2 wherein the disease associated antigen is selected from the group consisting of tumor-associated antigen, autoimmune disease-associated antigen, infectious disease-associated antigen, viral antigen, parasitic antigen and bacterial antigen.

5. The nucleic acid of claim 4 wherein said viral antigen is HIV antigen.

6. The nucleic acid of claim 5 wherein said HIV antigen is selected from the group consisting of Gag, Env, Pol, Nef, Vpr, Vpu, Vif, Tat and Rev.

7. The nucleic acid of claim 6 wherein the disease associated antigens comprise antigenic fragments of HIV Gag-Pol-Tat-Rev-Nef or Tat-Rev-Env-Nef linked together, not necessarily in that order.

8. The nucleic acid of claim 4, wherein said autoimmune disease-associated antigen is a T cell receptor derived peptide.

9. A vector comprising the nucleic acid construct of claim 1.

10. A host cell comprising the nucleic acid construct of claim 1.

11. A pharmaceutical composition comprising a nucleic acid of claim 1 and a pharmaceutically acceptable carrier.

12. A method of stimulating the immune response against an amino acid sequence of interest, comprising administering to a mammal a sufficient amount of pharmaceutical composition of claim 11 to stimulate an immune response.

13. A method for inducing antibodies in a mammal comprising administering to a mammal a composition of claim 11, wherein said nucleic acid construct is present in an amount which is effective to induce said antibodies in said mammal.

14. A method for inducing cytotoxic and/or helper-inducer T lymphocytes in a mammal comprising administering to a mammal a composition of claim 11, wherein said nucleic acid construct is present in an amount which is effective to induce cytotoxic and/or helper-inducer T lymphocytes in said mammal.

15. A vaccine composition for inducing immunity in a mammal against HIV infection comprising a therapeutically effective amount of a nucleic acid construct of claim 1 and a pharmaceutically acceptable carrier.

16. A method for inducing immunity against HIV infection in a mammal which comprises administering to a mammal a therapeutically effective amount of a vaccine composition according to claim 15.

17. A fusion polypeptide encoded by the nucleic acid construct of claim 1.

18. A viral particle comprising the nucleic acid construct of claim 1.

19. A pharmaceutical composition comprising the viral particle of claim 18.

20. A method of stimulating the immune response against a amino acid sequence of interest, comprising administering to a mammal a sufficient amount of pharmaceutical composition of claim 19 to stimulate an immune response.

21. A nucleic acid construct encoding a secreted fusion protein comprising a chemokine MCP-3 secretory leader amino acid sequence covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the secretory amino acid sequence.

22. A nucleic acid construct of claim 21 wherein the amino acid sequence of interest is a disease associated antigen.

23. A nucleic acid construct of claim 21 wherein the chemokine MCP-3 secretory leader sequence is MCP-3 amino acids 33-109 or 1-109.

24. A nucleic acid construct of claim 21 wherein the construct is selected from the group consisting of a (a) construct comprising a sequence encoding HIV p37 gag, a MCP-3 secretory leader sequence and a leader sequence of IP10 and (b) a construct comprising a sequence encoding SIV p39 gag, a MCP-3 secretory leader sequence and a leader sequence of IP10.

25. The nucleic acid construct of claim 22 wherein the disease associated antigen is selected from the group consisting of tumor-associated antigen, autoimmune disease-associated antigen, infectious disease-associated antigen, viral antigen, parasitic antigen and bacterial antigen.

26. The nucleic acid of claim 25 wherein said viral antigen is HIV antigen.

27. The nucleic acid of claim 26 wherein said HIV antigen is selected from the group consisting of Gag, Env, Pol, Nef, Vpr, Vpu, Vif, Tat and Rev.

28. The nucleic acid of claim 25 wherein the disease associated antigens comprise antigenic fragments of HIV Gag-Pol-Tat-Rev-Nef or Tat-Rev-Env-Nef linked together, not necessarily in that order.

29. The nucleic acid of claim 25, wherein said autoimmune disease-associated antigen is a T cell receptor derived peptide.

30. A vector comprising the nucleic acid construct of claim 21.

31. A host cell comprising the nucleic acid construct of claim 21.

32. A pharmaceutical composition comprising a nucleic acid of claim 21 and a pharmaceutically acceptable carrier.

33. A method of stimulating the immune response against an amino acid sequence of interest, comprising administering to a mammal a sufficient amount of pharmaceutical composition of claim 32 to stimulate an immune response.

34. A method for inducing antibodies in a mammal comprising administering to a mammal a composition of claim 32, wherein said nucleic acid construct is present in an amount which is effective to induce said antibodies in said mammal.

35. A method for inducing cytotoxic and/or helper-inducer T lymphocytes in a mammal comprising administering to a mammal a composition of claim 32, wherein said nucleic acid construct is present in an amount which is effective to induce cytotoxic and/or helper-inducer T lymphocytes in said mammal.

36. A vaccine composition for inducing immunity in a mammal against HIV infection comprising a therapeutically effective amount of a nucleic acid construct of claim 21 and a pharmaceutically acceptable carrier.

37. A method for inducing immunity against HUV infection in a mammal which comprises administering to a mammal a therapeutically effective amount of a vaccine composition according to claim 36.

38. A fusion polypeptide encoded by the nucleic acid construct of claim 21.

39. A viral particle comprising the nucleic acid construct of claim 21.

40. A pharmaceutical composition comprising the viral particle of claim 39.

41. A method of stimulating the immune response against an amino acid sequence of interest, comprising administering to a mammal a sufficient amount of pharmaceutical composition of claim 40 to stimulate an immune response.

42. A composition comprising a one or more vectors expressing different forms of an antigen covalently linked to destabilizing or secreting moieties.

43. A composition of claim 42 where at least one vector comprises a nucleic acid construct containing nucleotide sequences encoding a fusion protein comprising a destabilizing amino acid sequence covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence, and at least one vector comprises a nucleic acid construct encoding a secreted fusion protein comprising a secretory amino acid sequence covalently attached to a heterologous amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the secretory amino acid sequence.

44. A method for inducing antibodies in a mammal comprising administering to a mammal a composition of claim 42, wherein said vectors are present in an amount which is effective to induce said antibodies in said mammal.

45. A method for inducing cytotoxic and/or helper-inducer T lymphocytes in a mammal comprising administering to a mammal a composition of claim 42, wherein said vectors are present in an amount which is effective to induce cytotoxic and/or helper-inducer T lymphocytes in said mammal.

46. A method of claim 44 or 45 comprising administering the composition to the same site.

47. The method of claim 46 wherein the vectors are administered at the same time.

48. The method of claim 46 wherein the vectors are administered at different times.

49. A method of claim 44 or 45 comprising administering the composition to different sites.

50. The method of claim 49 wherein the vectors are administered at the same time.

51. The method of claim 49 wherein the vectors are administered at different times.

52. A composition comprising the vectors comprosing nucleic acids which encode wt gag, MCP3gag, and B-CATEgag.

53. A composition comprising the vectors wt env, MCP3env, and B-CATEenv.