BACKGROUND OF THE INVENTION
This invention relates to the field of contamination testing, particularly including the field of testing for biological contaminants.
This description is provided solely to assist the understanding of the reader, and does not constitute an admission that the cited references are prior art to the present invention.
In the processing of food materials and in the preparation of food products, as well as in other fields, it is advantageous to test for the presence of particular substances, often substances that would be regarded as contaminants. Materials detected as indicators of contamination include, for example, viable bacterial cells and ATP. For many substances, however, the commonly used detection methods involve a number of different procedural steps, such as reagent preparation, reagent mixing, sample transfer, and sample/reagent mixing. As an example, current methods for protein determination, which can serve as an indicator of a contaminated surface, often involve on-site reagent preparation due to stability problems, along with multiple transfer steps, and/or involve highly subjective color changes which make interpretation difficult, and/or require the use of complex instrumentation. Examples of common protein determination methods are described in Stoscheck, Quantitation of Protein, in METHODS IN ENZYMOLOGY Vol. 182, pp.50-68, 1990. Among the variants of basic protein detection methods are methods using colloidal forms of Coomassie® blue stain to detect proteins in gels such as polyacrylamide electrophoresis gels. Such methods are described, for example, in Neuhoffet al., 1985, Electrophoresis 6:427-488 and Neuhoffet al., 1988, Electrophoresis 9:255-262.
In addition, for tests performed in food processing facilities, contamination of food by assay reagents is a concern and the individuals performing the tests may lack significant experience preparing chemical formulations, potentially introducing significant test error. Therefore, the use of detection methods which require the preparation or transfers of assay chemicals is undesirable in many testing environments.
In addition to the conventional protein assay methods referenced above, a combination cleaning and protein staining composition is described in Winicov et al., U.S. Pat. No. 5,424,000, entitled ACID CLEANINGS AND STAINING COMPOSITIONS, issued Jun. 13, 1995. The solutions preferably include phosphoric, sulfuric, and nitric acids, and Acid Violet 19 dye.
A number of different self-contained sampling/testing devices employing certain assays have been described. Examples of such assays include sampling for bacterial contaminants in food processing plants, the sampling for contamination of the environment by heavy metals such as lead, and the collection of specimens from a patient to test for microorganism infection.
Specific examples of self-contained sampling/testing devices include Nason, U.S. Pat. No. 5,266,266, issued Nov. 30, 1993, and Nason, U.S. Pat. No. 4,978,504, issued Dec. 18, 1990, both entitled SPECIMEN TEST UNIT; Nason, U.S. Pat. No. 4,707,450, issued Nov. 17, 1987, entitled SPECIMEN COLLECTION AND TEST UNIT; and Tobin, U.S. Pat. No. 3,792,699, issued Feb. 19, 1974, all of which are hereby incorporated by reference in their entireties including drawings.
SUMMARY OF THE INVENTION
The need for, and utility of on site, immediate feedback to cleaning and audit personnel on the presence of residual contaminating substances in a variety of environments is well-established. For example, the need for contaminant monitoring has a well documented role in food safety programs when residual food residues can result in bacterial contamination and allergic responses in some individuals. Effective cleaning also reduces the risk of pathogens contaminating subsequent food products. A variety of devices and methods have been utilized for contaminant testing.
Particularly advantageous devices for the purpose of evaluating the presence of specific materials require no secondary reagents or steps, have easily detected changes in the presence of target material, give immediate results, and allow integrated collection of sample into the device. The present inventor shows that such a self-contained sampling/testing device can be constructed in which the presence of target material in a sample is detected calorimetrically through use of a dye which binds the target material. As indicated above, this device is particularly advantageous for routine sanitation testing procedures.
In a first aspect, the present invention concerns a self-contained device having a sampler for collecting a sample which may contain a target material, a signal generator having a contactable dye that binds to the collected target material, and a sampler washer having a wash solution for washing the collected target material and/or free dye from or on the sampler to facilitate measurement of a signal produced from the interaction of dye and target material. The sample collection surface or portion of the sampler is in communication with or can be placed in communication with the sampler washer. In preferred embodiments, the sampler collection surface is also in communication with or can be placed in communication with an absorbent material able to take up liquid from a wetting agent and/or dye solution and/or wash solution. The device may be constructed with any of many possible structural configurations, depending on the requirements of the particular application, e.g., depending on the specific type of dye used and the type of target material to be tested.
The term “in communication” refers to a contact or channel or other means that allows fluid contact between the referenced components. Thus, for example, a sampler washer and an absorbent material are in communication if fluid transport can occur from the sampler washer into the absorbent material. The term does not imply that fluid is actually present, but only that such fluid contact could occur if fluid were present.
In preferred embodiments, the device incorporates a target material precipitating dye, preferably a protein precipitating dye, for example, Ponceau-S dye. Such a dye binds to and precipitates, or assists in precipitating or keeping out of solution a target material. The sample collection surface of the sampler can be contacted with the dye ( in solution or dry) in a manner such that a quantity sufficient to dye target material in a sample is taken up by the sampler. In using such dyes, it is generally advantageous to separate bound dye from unbound dye to provide convenient detection of the presence of target material. Thus, preferred embodiments using such dyes employ an arrangement where the collected sample (which may contain target material) is or can be disposed between reservoirs such that wash solution can pass through or over a solid matrix carrying the collected sample. For example, the collected sample can be disposed between an absorbent material able to absorb wash solution and an absorbent material or other reservoir containing a wash solution. The saturation differential between these reservoirs provides for a directional transport of dye and wash solution across the collection pad surface. Preferably the wash solution is drawn through or over a matrix bearing a collected sample by capillary action. In embodiments where the collection surface and dry absorbent material are in direct contact, the dry absorbent material should have at least enough capacity to absorb sufficient dye and wash solution to wash one sample collection surface. In other embodiments, rather than involving capillary action in an absorbent material to draw wash solution through a sample-bearing matrix, a wash utilizes user-applied pressure that pushes wash solution through the sample bearing matrix.
The term “matrix” refers to a solid material suitable for retaining dye/target material complexes. In the context of this invention, a matrix is preferably, but not necessarily a porous matrix or porous material, meaning that the matrix is penetrated by a large number of passages of sufficient size to accept the passage of fluids such as water, but are preferably not so large that the matrix is free-draining. Such a porous matrix may be, for example, a network of interwoven fibers such as paper, cotton swab, or felt. Thus, the absorbent materials utilized in this invention, for example, for absorbing fluids to provide a flow through a sample collection surface are porous matrices or materials.
In the context of entrapment of complexes of target material and dye and the removal of unbound dye, the term “wash” or “washing” refers to a fluid transport of sufficient unbound dye to enhance the detection of complexes. It is understood that, in many cases, excess washing of dyed materials can remove bound dye in addition to unbound dye. Therefore, the washing is not so extensive that removal of bound dye interferes with the detection of the presence of target material using detection of the presence of dye retained in or on a solid support or matrix.
Preferably the sample collection matrix is an absorbent material, e.g., an absorbent pad, or the surface of an absorbent pad. In certain embodiments, the sample collection matrix binds the target material, in others the sample collection matrix entraps precipitated target material or the surface of the matrix retains dye/target material complexes.
In yet other embodiments, the device is arranged such that the sample collection matrix of the sampler is washed by wash solution by diffusion, which may be assisted by physical agitation. Generally in such embodiments, the sampler would then be removed from the dye-bearing wash solution. The target material, e.g., protein, would be bound to, entrapped by, or otherwise immobilized on or in a portion of the sampler.
By “entrapment” or “entrap” is meant a physical association, which may be chemical, electrostatic or steric in nature, such that a target material is retained in a matrix even in the presence of forces that otherwise might have a tendency to remove such target away from the matrix. This can occur, for example, through precipitation of target material such that the material becomes insoluble, e.g., using precipitating dyes such as Ponceau-S. In this way, washes may be performed to separate small, unreacted or unbound dye molecules from larger, dye/target material complexes, thus facilitating testing of samples.
The term “precipitate” or “precipitation” as used in the specification and claims includes the usual understanding of precipitation as a settling or deposit of solid particles out of solution. Additionally, the term as used herein also includes any general retention of solid or particulate matter, by any force, within, or in some cases on, an absorbent collection pad matrix or sampler or other solid phase surface. Thus, the definition includes but is not limited to target matter coming out of solution, target material agglutination, and target material conformational changes that act to obstruct the exit of these materials out of a matrix by creating complexes or other physical structures which cannot readily move through the pores of a porous material. Thus, those skilled in the art will readily be able to select appropriate materials and conditions for precipitation or other entrapment of a particular target material/dye combination, e.g., selection of a porous material with an appropriate average pore size which will allow target material to penetrate into the porous material, but small enough to prevent dye/target material complexes from quickly being transported out of the porous material.
In embodiments making use of a target material precipitating dye, e.g., protein precipitating dye, the dye stains/colors and immobilizes target material, e.g., a protein (e.g., protein adsorbed to or precipitated on an absorbent swab or pad). In this context, “immobilizes” means that the target material is removed from or prevented from entering the bulk of a solution (e.g., a dye solution or wash solution), such as by precipitation of the target material/dye complex, entrapment of the target material and/or target material/dye complex, or attachment of the target material to an insoluble or solid material, e.g., a particle, matrix, or support. In embodiments where target material binds to a matrix or surface, a precipitating dye need not actually precipitate a target material as it is immobilized by the binding to the solid matrix or support.
Certain of the embodiments described below demonstrate that the scope of the invention may also contemplate some minimum manipulation of device components and devices in which the device does not remain sealed after sample insertion and/or in which separate manipulation of one or more device components is needed.
Thus, the device in one embodiment includes a sampler for collecting a target material or contaminant, a signal generator for providing a target material binding dye, a sampler washer for washing unbound dye away from dye which is bound to target material, and at least one housing to contain the signal generator and sampler washer reagents.
In preferred embodiments, the sampler may take the form of a wand, stick or any other configuration that is suitable for taking up a particular type of sample. Such a sampler wand is generally a on-absorbent stick, preferably flattened, with a sample collection pad or surface on a terminal portion of the stick. The stick may also have an absorbent material in communication with the sample collection pad, e.g., on the other side of the same end of the stick, with communication through a hole or holes in the stick. Thus, in these configurations, there is an absorbent pad or material which is, or is adapted to be, juxtaposed to the collection surface for drawing dye and/or wash solutions across the target material, e.g., a protein contaminating a surface, and can facilitate entrapment of this target material on or within the pad matrix. The device incorporates a sampler washer to wash unbound dye from the sampler collection pad, preferably into an absorbent pad or reservoir, which in some embodiments, such as the wand, is juxtaposed to or integral with the sampler collection pad, and in others is an elongated extension of the sampler collection pad or an abutting absorbent material housed by a sampler stick or housing and providing for a flow of wash solution across the sample. Optionally, the device further includes a wetting agent or solution which can be utilized to wet the sample collection matrix or surface. Such wetting can assist in sample collection and/or in picking up a quantity of dry dye. A wetting solution can be the same or different from the wash solution. Thus, in applications where a moistened sample collection matrix is desired, the sample collection surface or matrix can be premoistened or can be moistened using a wetting solution.
In certain embodiments such as embodiments including a sampler wand, the sampler, or a portion of the sampler may be inserted into a “book” or hinged or flexible housing having a dye, wetting, and wash reagents, wherein a swabbed sample is subjected, either successively or at once, to the dye and wash reagents to effect not only a transfer of dye to the target material, but also the transfer away or separation of unbound or unreacted dye from target material. This is accomplished, for example, by using a saturated dye reservoir or pad or dry dye source, and a washing solution reservoir or pad contained in a housing, placing the sample to be tested successively on the dye and washing solution sources, and opposing the sample from the opposite side with a nonsaturated absorbent material which, upon proper stimulation, can receive fluid from the saturated source, in the process “washing” residual dye away from bound sample. Stimulation in this embodiment may occur by exerting pressure, for example by squeezing the sandwiched sample. It is not necessary that the reagent housing be hinged in book format; it could also be performed using separate and opposing saturated and nonsaturated absorbent materials. Alternatively, the sample is not opposed from opposite sides but rather is placed into a reservoir containing wash solution which flushes unbound dye from the sample collection pad.
As indicated above, in particular embodiments, a sampler wand may be constructed either with a sample collection pad but no additional absorbent material, or with both a sample collection pad and an absorbent material for drawing fluids through the sample collection pad, e.g., with a sample collection pad on one side in communication with an absorbent pad on the other side. Generally a sampler wand has a handle, preferably made of a non-porous material such as various plastics, coated papers, glass, or metal. Preferably the handle is at least two inches long, and more preferably 4, 6, or 8 inches long.
In other embodiments, such as ones including a sampler stick, there is no sandwich of the type described above. Rather, the body or housing of the sampler is hollow or integral with an internal reservoir adjacent or connected to the sample collection surface for receiving or flushing the dyed sample of unbound dye with washing solution.
Thus, the term “sampler stick” refers to an elongated housing structure which includes a sample collection surface or pad, and at least one reservoir. For example, a sampler stick may contain a wash solution, and optionally a wetting agent, along with a sample collection pad. The wetting agent or wash solution may be in continuous communication with the collection pad or may be separated with a separator until communication is desired. Alternatively, a sampler stick can contain a reservoir with dry absorbent material for absorbing wash solution in communication with the sample collection surface. Exemplary sample sticks are shown in FIGS. 3-5.
In certain embodiments, the device includes a housing with a plurality of reservoirs, e.g., three reservoirs containing wetting agent, dye (dry or in solution), and wash solution. After taking up a sample and a quantify of dye, the sample collection surface of the sampler is merely pressed against a wash solution reservoir to flush unbound dye from the sample, as the sampler itself, unlike the embodiments above, possesses a complementary receiving reservoir. The sampler stick formats, exhibited herein, are illustrative, but not limiting. As an alternative, the reservoir in the sampler stick can contain wetting/wash solution, and, following sample and dye uptake, the sample collection surface is pressed against an absorbent material in a housing to cause a flow of wash solution across the sample.
Such sampler sticks can utilize a housing containing reservoirs in a planar arrangement, e.g., as shown in FIGS. 3 and 4, or can use a housing in the form of a cap (e.g., FIG. 5). For example, such a cap can be reversible, such that in one orientation the cap seals and/or protects the sample collection surface. In the opposite orientation, the cap provides contact with dye and wash solution. Those skilled in the art will recognize that a variety of arrangements can be used to provide moistening of the sample collection surface, dye uptake or transport onto or into the sample collection surface and matrix, a source of wash solution, and a complementary absorbent material to receive wash solution as it washes the sample of unbound dye.
In some embodiments, certain of the reservoirs and/or reagents are contiguous or adjacent but separated by rupturable membranes or separators that, when broken, permit the flow of reagents across a collected/exposed sample to effectively wash the sample. FIG. 4 is exemplary but not limiting.
Some embodiments make use of solid dye which is hydrated and presented to a sample in response to a physical stimulation such as a rupturing of a membrane or membranes which maintain the dye in a dried, segregated state. FIG. 4 is illustrative, although by no means intended to be limiting. (The combined dye/wash solution reservoir in FIG. 4 could contain dry dye or a dye solution.) For example, a moistened sample collection surface can be touched to a dry dye such that a quantity of dye is transferred to the sample collection surface. The dye can contact target material directly and/or by fluid transport through a porous matrix to contact target material within the porous matrix.
The foregoing embodiments preferably utilize the properties of precipitating dyes. As illustrated by those embodiments, the invention also provides methods of using such dyes to fix or retard the egress of target materials, e.g., protein, from porous matrices into which target material has already been introduced, e.g. by swabbing. Thus, the introduction of target material as contemplated by the instant invention is not facilitated by or dependent on movement of particles or molecules in an electric field. Likewise, the method does not utilize a separation of components of a sample due to differential migration within the porous matrix. Instead, the matrix need merely be compatible in size to allow the initial ingress or association of target with matrix, and the influence of dye acts to thwart or inhibit the target material from leaving the matrix. This may be due, for example, to precipitation, conformational changes, agglutination, or any other result of dye binding which has the effect of sufficiently immobilizing target material in the porous matrix that unbound dye can be washed away and dyed target material visualized in the matrix. Alternatively, the immobilization can be due to chemical or electrostatic binding of target material to matrix. Further, matrix constituency is irrelevant as long as the criteria described above are met and as long as the dye is otherwise compatible with, or can be made compatible with, the matrices, e.g. with neutralizing agent. Illustrative but not limiting of the possible materials that may be used for the porous matrices are those discussed infra under the definition of “sampler”. The dye should not bind to the matrix material to such an extent that dye bound to target material in the matrix cannot be distinguished from dye binding to matrix.
In accord with the aspects above, the immobilization or entrapment of target materials with a solid matrix, e.g., in a porous matrix, provides a method for detecting the presence of target material in a sample. As previously indicated, the method involves entrapping or otherwise immobilizing target material/precipitating dye complexes on or within a solid matrix. For example, such complexes can be entrapped in a porous matrix by binding of target material with precipitating dye or by collection of dye/target material complexes on or in a collection surface or porous matrix. Generally, the method includes washing away unbound dye to allow convenient visualization or other detection, e.g., detection using an instrument such as a spectrophotometer or fluorometer. The method can involve various matrix materials, neutralizing agents, wash solutions, and dyes as described herein for other aspects.
For the methods herein involving precipitating dyes, azo dyes, preferably diazo dyes, which preferably have at least one, and preferably a plurality of sulfonic acid groups, e.g., 2, 3, or 4 groups (which may be prepared in the corresponding salt form) are preferred. The red dye, Ponceau-S, Sigma Chemical Co., St. Louis, Mo., (chemical abstracts service registry number 6226-79-5, [3-hydroxy-4-[2-sulfo-4-(4-sulfophenylazo) phenylazo]-2,7-naphthalenedisulfonic acid, tetrasodium salt], HOC10H4[N═NC6H3(SO3Na)(N═NC6H4SO3Na)](SO3Na)2, F.W. 760.58) is exemplary and most preferred. Ponceau-S is soluble in water, slightly soluble in ethanol, and insoluble in vegetable oils. It is stable at room temperature in acetic acid and in preferred embodiments is used to stain proteinaceous matter using a dye concentration of about 0.1-1.0% (w/v) in about 1-5% (w/v) acetic acid. The stain may be quickly removed upon addition of 0.1 N NaOH, or by excess wash solution. The terms “azo dye” and “diazo dye” have the meanings as generally accepted in the dye industry. The term “sulfonated” in connection with the dye compounds refers to the presence of sulfonic acid substituent groups. Such groups may be present in a corresponding salt form.
Advantageously, Ponceau-S binds rapidly to proteins and precipitates or immobilizes them in addition to staining/coloring them. Thus, such a precipitating dye is generally used to bind to and precipitate target material, e.g., protein, in or on a solid matrix. In such case, unbound dye is generally washed away from dye/protein complexes, providing visual detection of sample protein. In such embodiments the dye does not bind to the solid matrix to such an extent or under such conditions as to prevent or interfere with detection of dye/protein complex.
The invention provides a method for detecting protein on a solid surface. Preferably the method is applied in testing for contamination on the surface, e.g., food processing residue. The method involves contacting a solid surface, e.g. a metal surface, with a Ponceau S dye solution under conditions in which Ponceau S dye binds to protein. The rapid binding of Ponceau S to protein allows sufficient dye to bind to protein even on vertical surfaces. Preferably the method allows immediate visualization of protein-bound dye on the surface without further processing. If desired, the method can further include washing the surface with a wash solution which can wash away unbound dye. Preferably the dye is used at a concentration of 0.1-1.0% in dilute acetic acid. The dye solution and/or a wash solution can further contain neutralizing agent as described above.
Also in preferred embodiments of the present invention, the binding of dye to target material is detectable by a color change of the dye or dye solution, e.g., by a frequency shift of the dye on binding or a color change of a dye solution by dye depletion. Preferably a self-contained sampling/testing device incorporates a frequency shift dye. Frequency shift dyes have their absorbency or reflection or emission changed on interaction with target material, thereby differentiating bound from unbound dye. Such dyes can allow convenient detection of target material even without separation of bound and unbound dye.
Therefore, in preferred embodiments, the sample wash is able to transport sample material, e.g., target material from the collection portion or surface of the sampler. Device embodiments wherein liquid-phase analysis is performed typically employ a reading portion of the device that permits the sample reaction to be visualized or analyzed, with or without the aid of an instrument such as a spectrophotometer. The sample material is washed into the reading portion or alternatively carried into the reading portion on the sampler.
In certain embodiments employing frequency shift dyes or other dyes where a change in dye color is to be detected, the sampler may be contained within a lower housing that provides protection for the sampler from pre-testing contamination. Additionally, an upper housing may sealably engage the lower housing such that the two housings are in communication during the test. The sampler is preferably fixed to the upper housing. Within or comprising such housings may be a chamber or reservoir to hold a wash solution or a combined sample wash signal generator or separate reservoirs to hold each of a signal generator and a wash solution. A chamber may further include a breakable shaft contiguous with the chamber that, upon breakage, exposes an orifice through which the contained solution may flow to the sampler and may further flow to a read portion for evaluation.
Thus, the dye solution can flow through a hollow shaft in a swab in the device, flow through the swab tip removing the material adhering to the swab, and collect in a chamber of the device which is usable by the user for visual or instrumental detection of the intended dye reaction. Alternatively, the dye solution can wash a sample on or in a swab or other sampler, where the target material is detected on or in the sampler.
Frequency shift dyes provide convenient detection of bound dye even in the presence of unbound dye when the frequency shift is large enough to distinguish the two. In cases where an instrument is to be used to read the binding results, the frequency shift can generally be smaller than if a visual reading is to be utilized. For machine reading, preferably an absorption shift on binding (expressed as a wavelength shift) is at least 20 nanometers, more preferably at least 50 nm, still more preferably at least 75 nm, and most preferably at least 100 nm. For visual reading, preferably an absorption frequency shift on binding is at least 50 nm, more preferably at least 75 nm, still more preferably at least 100 nm, and most preferably at least 120 nm. For example, an absorption peak of Coomassie blue stain under acidic conditions shifts from about 465 nm to about 595 nm on binding of the dye to protein. For a visual reading it is preferable if the absorbance change produces a color change rather than just a shade change. For example, the GelCode® reagent changes from amber to blue on protein binding. A fluorescent emission shift is preferably at least 20 nm, more preferably at least 40 nm, still more preferably at least 75 nm, and most preferably at least 100 nm.
Gelcode® includes colloidal Coomassie® G-250 dye. Colloidal Coomassie® blue dyes may also be formed as described in the art. For example, in Neuhoff, et al., 1985, Electrophoresis 6:427-448 and in Neuhoff, et al., 1988 Electrophoresis 9:255-262. In general, these solutions utilize Coomassie blue dye® in an acidic aqueous solution with ammonium sulphate or ammonium iron sulphate. In one example, the solution contains 0.1% weight/volume (w/v) Coomassie blue G-250 in 2% w/v phosphoric acid, and 6% w/v sulphate. In an alternative solution, the dye contains 10% w/v ammonium sulphate and 20% w/v methanol. Preferably, the pH of the solution is between 1 and 2 and the ammonium sulphate or ammonium iron sulphate concentration is between 2% and 15% more preferably between 4 and 10%, and most preferably between 5 and 8% w/v. The pH should not be so low that the dye molecules are rapidly degraded and the ammonium sulphate concentration should be selected so that the solution takes on a color characteristic or the colloidal form, preferably the majority of the dye molecules are present as colloidal particles rather than being in free solution or precipitating out of solution.
As appreciated by one of ordinary skill in the art, certain device embodiments will accommodate the use of various types of target material binding dyes, e.g., precipitating dyes or frequency shift dyes. For example, embodiments which utilize a wash to carry sample material away from a sampler can be utilized with a frequency shift dye. Such devices can also be used with a precipitating dye where there is the capability to wash unbound dye away from target material bound dye in or on the sampler or on a porous separator. In accord with certain embodiments described herein which incorporate a wash solution in a reservoir in a sampler portion or upper housing, sample can be collected on a sampler, contacted with dye, e.g., from a reservoir in the sampler portion or upper housing or by dye contained in the sampler prior to sample collection, and then washed by a wash solution contained in a reservoir in the sampler portion or upper housing. Preferably in such embodiments, the collection surface of the sampler is pre-moistened. Similarly, a device in which a dye solution is in a reservoir contactable with a sample collection surface (see e.g., FIG. 2 below) can be used with a frequency shift dye or with a precipitating dye. With either type dye, sample material is transferred to contact the dye on the sampler. For a frequency shift dye, dye binding to target material is detected by a color change of the dye as dye binds target material in the dye solution or on the sampler, or as dye is depleted from the solution as dye binds to target material in or on the sampler.
The terms “sampling/testing device” or “self-contained sampling/testing device” indicate that the device is constructed so that all components for a particular assay are provided within a single device along with a means for introducing a sample into the device. It may, however, be advantageous for certain embodiments to utilize separate apparatus for incubation during the assay or for reading results of the assay.
By “sampler” is meant a device component (or components) which allows one to obtain all of or a portion of a sample which may be present on a surface, in a solution or in an atmosphere to be tested. For example, the sampler may be an absorbent pad or a swab with a shaft and an absorbent tip. The shaft of the sampler or the sampler stick housing may be hollow, and may further include a vent. As alternatives, the sampler/swab may take the form of a Q-Tip® or a simple pad. The swab may include natural or synthetic materials so long as deposition of a sample thereto may occur and dye binding to the swab does not interfere with detection of the target substance so as, to prevent such detection. The absence or reduction of such interference may be provided, for example, by selection of material and/or by the physical interrelationships of device components. The material may be but is not limited to sponge, mylar, nylon, dacron, rayon, porex, porous polypropylene, porous polyethylene, glass fibers, paper, or various other woven or felted fibers such as nitrocellulose, cotton, wool, cellulose, or combinations thereof. In a preferred embodiment in which the swab includes a shaft, the swab shaft is preferably hollow, allowing the sample wash and/or dye solution to flush the collected sample material from the swab into a reaction and/or read chamber. Preferably the swab is provided for use in a pre-moistened form to assist in solubilizing and absorbing sample material into the sampler, or can be readily moistened from a reservoir within the device containing a wetting solution, e.g., in a saturated absorbent matrix. The moistening fluid may be a buffer, water, acid, or base depending on the type of dye used. Those skilled in the art understand the selection of a compatible moistening fluid for the dye and target material involved in a particular type of test. The sampler may function through capillary action, for example a capillary tube or tubes. The sampler may comprise a pipetting means. The sampler may comprise a chamber that captures a sample of an atmosphere, such as the atmosphere present in an enclosed work space. The sampler may assume virtually any shape or combination of shapes, e.g., planar, elongated, circular, elliptical, cylindrical, spherical, cubical, conical, etc. Preferably the sampler is designed to enable a user to conveniently reach into locations in equipment, such as food processing equipment which are difficult to access. Thus, the sampler is preferably constructed to provide an extension with a thin cross-section, e.g., a cross-sectional area of less than 2 in2, more preferably less than 1 in2, and in certain embodiments less than ½ in2. Such extension is preferably at least 2 inches in length, more preferably at least 4, 6, or 8 inches in length. Such extension may be provided, for example, by a wand handle, a swab shaft, or an elongated housing, or combinations.
The term “sampler portion” refers to a structural assembly which includes a sampler and also includes additional components which allow the sampler to be sealed or attached to the remainder of the device, and may also include one or more reagent spaces, such as a reservoir for a sample wash solution.
By “sampler washer” is meant a device component (or components) which allows the removal of all or a part of a sample present on the “sampler”. For example, in some embodiments an upper housing or sampler portion or sampler stick comprises a chamber as a reservoir containing a fluid in which the fluid may be selectively released as desired, ordinarily to release a sample that has been obtained or to wash unbound dye away from dye/target material complexes. In an alternative embodiment, the upper housing or sampler portion may contain a container such as, but not limited to, an ampule or a packet. The ampule or packet may contain a fluid as described above, which may be selectively released. In an alternative embodiment the upper housing or sampler portion may contain two containers, both or either comprising, for example but not limited to, an ampule or packet containing the same or different fluids or dry substances. In another embodiment a fluid may be directly contained in the upper housing or sampler portion and a container or containers containing a fluid or dry substance (or more than one fluid or dry substance) be contained therein. In yet other alternatives, the lower portion of the housing or the lower housing may contain a fluid that is used to wash the sample from the sampler, for example, by inserting the end of the sampler into the fluid or otherwise forcing the fluid against or through the sampler.
By “wash solution” is meant a solution, e.g., an aqueous solution, capable of separating unbound dye from dye/target material complexes and/or carrying sample materials from a sampler to another location. The solution may also serve the function of a wetting agent, e.g., for moistening a swab or collection surface in anticipation of or facilitation of a sample collection. A wash solution for use with a particular dye does not contain such amount of agents which tend to disrupt binding of that dye to target material that determination of target material binding is prevented. Preferably no such agents are present, but in some cases it may be desirable to include a low level of such an agent or agents, for example, to minimize binding of dye to a porous matrix, thereby enhancing contrast and improving target material detection. Those skilled in the art will readily be able to select an appropriate wash solution for a particular dye. In embodiments utilizing Ponceau S dye, the wash solution and/or wetting agent are preferably dilute acetic acid solutions, preferably with 0.1 to 10% acetic acid in water, more preferably 0.5 to 5%, still more preferably 1.0 to 5% acetic acid.
By “signal generator” is meant a chemical compound or physical stimulus or biological agent that provokes a measurable or discernable response in the presence of a target material; by chemical compound is meant a chemical dye, an enzyme, or other organic or inorganic structure capable of inducing such response.
The term “target material binding dye” or “dye” refers to a compound which will preferentially bind to a target material in a sample as compared to binding to other molecules which are likely to be present in such samples. Thus, the dye may bind to other molecules at a level equal or greater than that for binding to the target material, but such other molecules are ones which are generally not present in samples to be tested, such as samples to be tested in evaluating process contamination. The preferential binding need not occur under all conditions, but at least occurs under the assay conditions selected for use in the device of the present invention. The dye compound also is detectable using visual or spectroscopic means, and preferably absorbs or fluoresces at visible wavelengths so as to give a characteristic color. Binding of the dye to target material, e.g., protein, preferably results in a color change and/or precipitation of the protein that is visible. Thus, for example, the term “protein-binding dye” refers to a compound that preferentially binds to protein, polypeptides, or oligopeptides in preference to other molecules. The dye, while active in aqueous form may be initially dry and hydrated during the testing process, e.g., when contacted with wetting/washing solution.
By “frequency shift dye” is meant a composition which upon interaction with the substance to be detected exhibits a characteristic detectable change in the light emission or absorption spectrum of the dye molecule. Preferably the alteration of the light absorption characteristics of the dye molecule is observed. Changes in absorption or emission spectra can include, for example, the appearance or increase of absorbance or emission peaks or bands, the disappearance or reduction of absorbance peaks or bands and combinations of these. Preferably the frequency shift dye is a protein binding dye that is colloidal such as Coomassie® blue dye, preferably GelCode® Blue Stain Reagent, Pierce Chemicals, Rockford, Ill.
The term “colloidal dye” refers to a dye which is in a finely divided state in a liquid, such that the solid particles of dye are in the range of 1 to 1000 nanometers, preferably in the range of 1-100, and more preferably in the range of 5-100 nanometers. This does not mean that all of the dye present in the liquid is in the form of such particles, as those skilled in the art recognize that the colloidal form is generally in thermodynamic equilibrium with solubilized dye and/or with solid dye particles larger than colloid size, e.g., larger than 1000 nanometers. Most useful are colloidal dyes where the amount of dye in colloidal form is sufficient to alter the color of the dye solution as compared to solutions containing the same amount of dye but in which the dye is in solution and/or in non-colloidal particles. In preferred embodiments, at least 30% of the dye molecules are in colloid size particles, preferably at least 50%, and more preferably at least 70% or 90%. As recognized by those skilled in the art, a transition from soluble form to colloidal form of a molecule in liquid solution can be monitored by an increase in light scattering for the solution.
The term “swab” as used in the claims is used as a noun to denote an absorbent and/or adhesive pad that serves to collect sample target material in prelude to or concurrent with exposure to a signal generator, i.e., a dye.
By “neutralizing agent” is meant a chemical compound or solution that helps to neutralize potentially interfering compounds present on the surface being tested or in a wetting agent present in or on a sampler collection surface, which compounds may interfere with the dye binding to the target material, e.g., protein. Exemplary neutralizing agents include sodium thiosulfate, MgCl2, and Triton-X® (Octoxynols; α-[4-(1,1,3,3,-Tetramethylbutyl)phenyl]-w-hydroxypoly(oxy-1,2-ethanediyl). All are available from Sigma, St. Louis, Mo. Triton-X® can be used, preferably at an effective concentration of about 0.01-0.5% weight volume. Sodium Thiosulfate may be used, preferably at an effective concentration of about 0.01-1.0 mg/ml. MgCl2 is preferably used at a concentration of 0.1-20 mg/ml..These neutralizing agents may be incorporated into any, all, or a combination of the wetting, wash, dye, or sample solutions provided. One of skill in the art will recognize that other neutralizing agents may be substituted provided they do not interfere with the signal generator mechanism and measurement, and will understand what neutralizing agents are appropriate for a particular application or can determine whether or not a potential compound is appropriate by simple testing.
Thus, by “neutralize” is meant to inactivate potentially interfering compounds present on the sample surface without disrupting the signal generator's function in combination with the target material and the rest of the device.
By “effective concentration” is meant one that supplies, in whole or in part, the intended or desired effect, e.g., the desired neutralizing effect.
The term “participates” as used in the claims denotes an assistance in the movement and/or gathering of target material onto the sampler, for instance by pre-moistening of an absorbent collection swab or pad.
By “reading portion” is meant a distinct section of the device housing wherein a reading or measurement or detection may be taken.
The term “in succession” connotes a temporal order but does not preclude the use of wash solution as a wetting agent for a sampler swab in prelude to exposure to dye. Thus, the wash solution may be used twice, both before and after the dye.
The terms “contacted with”, “contacted by” or “on contact with” denotes the direct or indirect touching of one object with another. Certain embodiments have the contact mediated through a pierceable membrane, which is rupturable by the sampler to effect the dyeing and washing of a target material presented on the sampler.
The term “segregates” as used herein denotes a separation and/or containment which may be undone upon proper stimulation, for example the piercing of a membrane by a sampler to allow mixing of components from each side of the membrane.
By “stably packaged” is meant that the dye or other signal generating component may be stored prior to use for prolonged periods of time, for example, a year or more if stored at 4° C., and still provide a signal upon activation. In one embodiment of the invention the signal generating means, e.g., comprising a colloidal dye solution, is stably packaged within a sealed glass ampule. The ampule may be a borosilicate glass, for example Pyrex®. It may be an “onionskin” type of glass ampule. In other embodiments, a signal generating component, e.g., a dye, is sealed within a chamber by a membrane or membranes.
As understood by those skilled in the art, the stability of a dye molecule will depend on the storage conditions, thus, the storage form can be varied as appropriate for a particular dye. If the dye is sufficiently stable in the test solution, the dye solution can be packaged in the device as a single solution. Alternatively, if the dye is not sufficiently stable in the assay solution, the stability can be enhanced by packaging the dye within the device separated from one or more other components of the test solution until mixing is desired. Thus, for example, the dye and/or components decreasing dye stability can be separated within the device by any of a variety of methods, such as by using separate reservoirs or capsules or ampules or separators or combinations thereof, such that one or more can be ruptured, broken, or opened to allow mixing of various components at a desired time or times.
By “separator” is meant a device component(s) or structure for separating two portions of the device, e.g., for separating the region containing the sampler from the region in which detection is performed until introduction of the sample into the detection region (read portion) is desired or for separating a sample on the sampler from a dye solution until contact between the sample and the dye is desired. For example, a separator may be a porous plastic or hydrophobic material filter, however, the porosity is not such that the sample would filter through without the application of a force, other than gravity, on the sample. As further examples, the separator may be a one way valve, or a puncturable membrane or a breakable or rupturable reservoir or capsule or ampule.
A “reservoir” may be a well, ampule, recess, void, or chamber capable of holding a liquid or solid. Such reservoir may be encased or contained by a rigid, soft, or flexible housing such as a plastic. A reservoir may be or include an absorbent pad that is saturated or capable of absorbing solution or solutions, e.g., target material, dye, wash, wetting agent, or combinations thereof. Such absorbent material is preferably located in a depression, void, chamber, cavity or the like of a housing.
Preferably the dye and test conditions are selected such that a readable result is provided within one hour at room temperature, more preferably within 30 minutes or 20 minutes, still more preferably within 10 minutes or 5 minutes, and most preferably within 2 minutes or 1 minute. Such rapid results are particularly advantageous for field sanitation testing as retention of the samples for long periods of time is not required and stability or consistency of the read of the completed test is enhanced.
As indicated above, a particularly advantageous embodiment of the present invention is adapted for protein detection, and therefore provides for the rapid and convenient testing of surfaces or solutions for contamination by protein-containing substances. The presence of protein can be a good indicator of residual food contamination remaining after cleaning procedures have been completed, as protein is a component of many food products. For example, in one application, the device or method will allow for testing of surfaces in food production plants, supermarkets and restaurants to ensure that cleanup procedures after food processing have been effective. Certain preferred embodiments utilize a dye capable of precipitating as well as staining protein, for example, Ponceau-S. Ponceau-S is particularly useful due to its speed of staining as well as its ability to both precipitate and stain protein. Other embodiments are slower and utilize colloidal protein binding dyes via a single step, integrated sampling assay device with visually distinct color changes in the presence of small amounts of protein material, e.g. colloidal Coomassie® blue such as found in GelCode® Blue Stain Reagent. Colloidal Coomassie Blue imparts a convenient spectral or color shift in the presence of protein.
By “protein” is meant peptide polymers (i.e., polymers of amino acids) and thus includes oligopeptides, full-length cellularly-produced polypeptides, degraded cellular polypeptides, complexes of polypeptides, and polypeptides associated with other molecules.
In preferred embodiments, the results of a test using the device can be read visually. In other embodiments, the result can be read in an instrument, such as a spectrophotometer or colorimeter. These devices are most useful for applications employing frequency shift dyes.
The device in a preferred embodiment includes a sampler and a combined sample treatment, sample wash and signal generator stably packaged, preferably allowing easy visual interpretation.
By “a combined sample treatment, sample wash and signal generator” is meant components or structures to contain a target material binding dye, which preferably either precipitates and stains proteins, or else creates a frequency shift on contacting protein, e.g., colloidal dyes. In either case, the dye solution can be released at will to wash a sample on or from the sampler thereby signaling the presence, absence, or quantity of protein present. In preferred embodiments the solution collects in a reading portion of the device. The dye solution or wetting agent can also treat the sample in a desired manner, for example, by solubilizing or permeabilizing cell walls and/or membranes of microorganisms (e.g., bacteria and fungi) or other cells.
By “fix” is meant that target material in the presence of a precipitating dye, e.g. Ponceau-S, is relatively slowed or halted from diffusing from or otherwise exiting the porous matrix in which it has entered or is contained, as compared with target material/matrix in the absence of dye.
Other specific applications of the invention include but are not limited to the following: the testing of surfaces for other types of contamination such as carbohydrates, lipids and microorganisms; the testing of liquid solutions for the presence of proteins, carbohydrates, lipids and microorganisms; the testing of air or gas for proteins, carbohydrates, lipids and microorganisms; and the testing of other materials such as dirt, vegetable material, manufactured articles, spices, powders, chemicals, debris and other types of samples familiar to those skilled in the art for such contaminants as protein, carbohydrates, lipids, nucleic acids, microorganisms, toxins, poisons, byproducts, adulterants and other materials recognized by those skilled in the art and capable of binding to dyes or ligands capable of being contained in colloidal or other forms which sequester or contain such reagents so that reaction with specific target materials results in or can be stimulated to result in rapid, detectable and distinct changes.
In accord with the provision of sampling/testing devices as described above, in another aspect, the invention provides a method of making such a test device by depositing a target material binding dye, preferably a precipitating-type dye or a frequency shift dye within a reservoir in a self-contained sampling/testing device. As indicated above, for device embodiments which include a sampler portion and a housing, the dye solution may be deposited in a reservoir in the sampler portion or alternatively in the housing. In embodiments in which the device includes an upper housing and a lower housing, the reservoir may be in the upper housing or alternatively in the lower housing. The dye used is preferably a precipitating dye, such as Ponceau-S. Alternatively, a colloidal dye such as colloidal Coomassie® blue is used which exhibits a frequency and color shift change on contact with protein.
In another aspect, the invention provides a method for detecting the presence of a substance, i.e., a target material, by using a sampling/testing device as described above. Thus, in preferred embodiments, the method involves obtaining a sample which may contain the substance to be detected (i.e., the target material) on or in the sampler. Depending on the type of sample, this may, for example, involve swabbing a surface, depositing a solubilizing or suspension liquid on a surface and then taking up at least a portion of that liquid, or taking up a sample of liquid from a bulk liquid, such as by pipetting or in a capillary tube or by moistening an absorbent material. The sample is contacted with a target material binding dye, e.g., a precipitating dye or a frequency shift dye, as described above within the device, and the presence of the target material in the sample is determined by detecting the occurrence of a dye color change, e.g., a dye frequency shift, by observing a visually detectable change in the color or shade of the dye solution following contact with the sample or by reading a change or changes in an absorbence or emission spectrum of the dye in a reading instrument following contact with the sample or by detecting the presence of bound dye on or in a matrix which immobilizes dye/target material complexes but which allows unbound dye to be separated, e.g., by washing away.
In particular, a method of sanitation testing is provided in which a device as described above is used to detect the presence of contaminants on or from a surface or in solutions, such as following cleaning procedures on a surface. In particular embodiments, the method involves contaminant testing of surfaces or solutions in a food processing facility such as a food production plant, or a restaurant. In preferred embodiments, the contaminant to be detected is protein food residue.
Other and further objects, features and advantages will be apparent from the following description of the presently preferred embodiments of the invention and from the claims.