TWI862597B - 新穎二維lc-ms/ms系統及相關方法 - Google Patents

新穎二維lc-ms/ms系統及相關方法 Download PDF

Info

Publication number
TWI862597B
TWI862597B TW109118223A TW109118223A TWI862597B TW I862597 B TWI862597 B TW I862597B TW 109118223 A TW109118223 A TW 109118223A TW 109118223 A TW109118223 A TW 109118223A TW I862597 B TWI862597 B TW I862597B
Authority
TW
Taiwan
Prior art keywords
column
solvent
sample
analytes
acetonitrile
Prior art date
Application number
TW109118223A
Other languages
English (en)
Chinese (zh)
Other versions
TW202111322A (zh
Inventor
偉廉 莊
菲力普 加佐茲 洛普茲
喬許華 帕凱可
傑若 山德林
Original Assignee
美商建新公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 美商建新公司 filed Critical 美商建新公司
Publication of TW202111322A publication Critical patent/TW202111322A/zh
Application granted granted Critical
Publication of TWI862597B publication Critical patent/TWI862597B/zh

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B01D15/1878—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series for multi-dimensional chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/322—Normal bonded phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/89—Inverse chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/08—Sphingolipids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Endocrinology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Electron Tubes For Measurement (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
TW109118223A 2019-05-31 2020-05-29 新穎二維lc-ms/ms系統及相關方法 TWI862597B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962855636P 2019-05-31 2019-05-31
US62/855,636 2019-05-31

Publications (2)

Publication Number Publication Date
TW202111322A TW202111322A (zh) 2021-03-16
TWI862597B true TWI862597B (zh) 2024-11-21

Family

ID=72193559

Family Applications (1)

Application Number Title Priority Date Filing Date
TW109118223A TWI862597B (zh) 2019-05-31 2020-05-29 新穎二維lc-ms/ms系統及相關方法

Country Status (14)

Country Link
US (2) US12292424B2 (https=)
EP (2) EP3976218B1 (https=)
JP (3) JP7568648B2 (https=)
KR (2) KR20260040656A (https=)
CN (2) CN119861166A (https=)
AU (1) AU2020282831B2 (https=)
CA (1) CA3140183A1 (https=)
DK (1) DK3976218T3 (https=)
ES (1) ES3026686T3 (https=)
HU (1) HUE071194T2 (https=)
IL (2) IL288497B1 (https=)
PL (1) PL3976218T3 (https=)
TW (1) TWI862597B (https=)
WO (1) WO2020243502A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120254137B (zh) * 2025-06-05 2026-04-14 科诺美(北京)科技有限公司 辅酶q10的检测方法及其应用
CN120820662B (zh) * 2025-09-17 2025-11-18 岛津企业管理（中国）有限公司 一种同步分析检测水产品中代谢物和脂质的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160054273A1 (en) * 2014-08-20 2016-02-25 Korea Research Institute Of Standards And Science Two-dimensional liquid chromatography system for heart-cut method
TW201736846A (zh) * 2016-04-01 2017-10-16 行政院農業委員會農業藥物毒物試驗所 搭配質譜影像分析檢驗農藥殘留之方法及其系統
US20190064128A1 (en) * 2016-01-29 2019-02-28 Ge Healthcare Bio-Sciences Ab Liquid Fraction Collector for Liquid Chromatography System
TW201908731A (zh) * 2017-06-02 2019-03-01 美商健臻公司 使用質譜絕對定量低豐度多肽的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0998229A4 (en) * 1997-06-17 2001-04-04 Cool Laser Optics Inc METHOD AND APPARATUS FOR REGULATING BIOLOGICAL TISSUE TEMPERATURE BY SIMULTANEOUS IRRADIATION
WO2000045929A1 (en) * 1999-02-08 2000-08-10 Admetric Biochem Inc. Chromatographic system with pre-detector eluent switching
JP2003028849A (ja) 2001-07-10 2003-01-29 Hiromasa Tojo 自動化脂質組成一斉分析装置
JP2004184215A (ja) 2002-12-03 2004-07-02 Shimadzu Corp アクリルアミドの分析方法及び装置
EP1635174A1 (en) 2003-02-28 2006-03-15 Japan Science and Technology Agency Method of separating sugar from compound having glycoside bond, sugar separation system, sugar separation agent kit, standardized sample for sugar separation and assessment system
KR100614099B1 (ko) 2005-11-25 2006-08-22 한국과학기술연구원 뇨 또는 플라즈마 시료 중의 디에이치이에이 설페이트의검출방법
JP4790435B2 (ja) * 2006-02-03 2011-10-12 株式会社日立ハイテクノロジーズ ３次元液体クロマトグラフィ
US20080183401A1 (en) * 2007-01-29 2008-07-31 Teledyne Isco, Inc. Apparatuses and methods for wireless monitoring and control of enviromental sampling and chromatographic apparatuses
JP5054439B2 (ja) 2007-06-15 2012-10-24 株式会社日立ハイテクノロジーズ イオン交換および順相カラムによる２次元液体クロマトグラフ
DE102008013500A1 (de) * 2008-03-10 2009-09-17 Evonik Degussa Gmbh Neue chirale Selektoren und stationäre Phasen zur Trennung von Enantiomerengemischen
WO2012129076A1 (en) * 2011-03-23 2012-09-27 Idex Health & Science Llc Valve and splitting system for multi-dimensional liquid analysis
ES2731920T3 (es) 2013-05-14 2019-11-19 Centogene Ag Método para el diagnóstico de la enfermedad de Niemann-Pick
LT3004896T (lt) * 2013-06-07 2020-01-10 Genzyme Corporation Rūgštinės sfingomielinazės sutrikimų žymuo ir jo panaudojimas
JP6385103B2 (ja) * 2014-03-31 2018-09-05 株式会社島津製作所 順相・逆相カラムを備えた超臨界流体クロマトグラフとそれを用いた分析方法
US20160195502A1 (en) * 2014-12-31 2016-07-07 Castle Medical, LLC Method for chiral separation of methamphetamine and amphatamine enantiomers
WO2016160544A1 (en) * 2015-03-27 2016-10-06 The Scripps Research Institute Lipid probes and uses thereof
JP6716087B2 (ja) 2016-03-25 2020-07-01 公立大学法人大阪 分析方法及び分析装置
US20180306797A1 (en) 2017-04-24 2018-10-25 Washington University Methods of detecting ceramide
KR20250025051A (ko) * 2017-08-24 2025-02-20 사노피 산성 스핑고미엘린분해효소 결핍증 환자에서의 비정상적 골병태의 치료
CN109187822A (zh) 2018-11-22 2019-01-11 上海烟草集团有限责任公司 一种中心切割二维色谱质谱联用分析烟草中农残的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160054273A1 (en) * 2014-08-20 2016-02-25 Korea Research Institute Of Standards And Science Two-dimensional liquid chromatography system for heart-cut method
US20190064128A1 (en) * 2016-01-29 2019-02-28 Ge Healthcare Bio-Sciences Ab Liquid Fraction Collector for Liquid Chromatography System
TW201736846A (zh) * 2016-04-01 2017-10-16 行政院農業委員會農業藥物毒物試驗所 搭配質譜影像分析檢驗農藥殘留之方法及其系統
TW201908731A (zh) * 2017-06-02 2019-03-01 美商健臻公司 使用質譜絕對定量低豐度多肽的方法

Also Published As

Publication number Publication date
JP7804023B2 (ja) 2026-01-21
PL3976218T3 (pl) 2025-06-09
CA3140183A1 (en) 2020-12-03
AU2020282831B2 (en) 2026-03-05
JP2026062952A (ja) 2026-04-10
ES3026686T3 (en) 2025-06-11
EP3976218B1 (en) 2025-02-19
AU2020282831A1 (en) 2021-12-16
IL288497B1 (en) 2026-04-01
KR20260040656A (ko) 2026-03-25
EP4560320A3 (en) 2025-08-13
JP7568648B2 (ja) 2024-10-16
IL326850A (en) 2026-04-01
IL288497A (en) 2022-01-01
JP2025000908A (ja) 2025-01-07
EP4560320A2 (en) 2025-05-28
CN119861166A (zh) 2025-04-22
KR20220016103A (ko) 2022-02-08
US20220252559A1 (en) 2022-08-11
DK3976218T3 (da) 2025-05-05
JP2022534363A (ja) 2022-07-29
CN114364980B (zh) 2024-12-27
KR102936574B1 (ko) 2026-03-09
WO2020243502A1 (en) 2020-12-03
CN114364980A (zh) 2022-04-15
TW202111322A (zh) 2021-03-16
US12292424B2 (en) 2025-05-06
US20250327780A1 (en) 2025-10-23
HUE071194T2 (hu) 2025-08-28
EP3976218A1 (en) 2022-04-06

Similar Documents

Publication Publication Date Title
JP7804023B2 (ja) 二次元ｌｃ－ｍｓ／ｍｓシステム
JP6092890B2 (ja) 質量分析による逆トリヨードサイロニンの検出法
JP7515672B2 (ja) 多重化した患者サンプル中のテストステロンのマススペクトロメトリーによる決定
Hendrickx et al. Recent advances in the application of hydrophilic interaction chromatography for the analysis of biological matrices
US11624737B2 (en) Methods for detecting lacosamide by mass spectrometry
JP2025118723A (ja) マススペクトロメトリーによるクロモグラニンａの検出方法
Aarika et al. From complexity to clarity: expanding metabolome coverage with innovative analytical strategies
Lee et al. On-line high speed lipid extraction for nanoflow liquid chromatography-tandem mass spectrometry
HK40126724A (en) Two-dimensional lc-ms/ms systems
HK40063702A (en) Two-dimensional lc-ms/ms systems
HK40063702B (en) Two-dimensional lc-ms/ms systems
CN121620698A (zh) 通过质谱法检测儿茶酚胺的方法