TWI731028B - 用以篩選核酸適體之方法 - Google Patents

用以篩選核酸適體之方法 Download PDF

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TWI731028B
TWI731028B TW106102762A TW106102762A TWI731028B TW I731028 B TWI731028 B TW I731028B TW 106102762 A TW106102762 A TW 106102762A TW 106102762 A TW106102762 A TW 106102762A TW I731028 B TWI731028 B TW I731028B
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Taiwan
Prior art keywords
dna
nucleic acid
selex
aptamer
sequence
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TW106102762A
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TW201803991A (zh
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吉本敬太郎
和久井幸二
古性均
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國立大學法人東京大學
日商鏈結生物股份有限公司
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Priority to JP2016-010415 priority Critical
Priority to JP2016010415 priority
Application filed by 國立大學法人東京大學, 日商鏈結生物股份有限公司 filed Critical 國立大學法人東京大學
Publication of TW201803991A publication Critical patent/TW201803991A/zh
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
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