TWI670067B - Wild bitter gourd extract technology - Google Patents

Abstract

The invention relates to an extraction technology of an extract of Momordica charantia L. with treatment for diabetes. The extraction process mainly comprises pretreatment and extraction of bitter gourd, and the bitter melon extraction is mainly carried out by input circulation tank, ultrasonic extraction and extraction extraction. The composition comprises the steps of: inputting the treated bitter gourd powder into a circulation tank of an extracting machine, and performing ultrasonic concentrated extraction, wherein the ultrasonic concentrated extraction system concentrates the ultrasonic wave on a predetermined time of the circulation tank The position is such that the bitter gourd powder can be shattered by the concentrated ultrasonic waves at the position, and the extract can be taken out after the extraction is completed, and the ultrasonic concentrated extraction method is adopted to effectively increase the extraction rate and reduce the production cost.

Description

Extraction technology of extract of Momordica charantia L. with diabetes

The invention relates to an extraction technology of a mountain bitter gourd extract for treating diabetes, and refers to a technique and a method for extracting bitter gourd, and the extract thereof can be effectively used as a health food or medical drug for treating diabetes.

Diabetes is one of the most common metabolic diseases today. According to the World Health Organization, more than 150 million people have diabetes in the world, 95% of whom are of the second type. In diabetes, type 2 diabetes occurs as a loss of beta cell function and insulin resistance. Pathological phenomena include increased glucose tolerance, increased insulin in the blood, and pancreas and kidney damage. At present, the drugs for diabetes are still insufficient. It is urgent to develop new drugs. It is an important research direction to develop economical and efficient lead drugs from medicinal plants with hypoglycemic activity. Plants synthesize diverse compounds for their structural and functional purposes, and many of these abundant molecular types are often used as therapeutic agents for health and maintenance purposes. Many clinical studies have reported that Cucurbitaceae plants have Hypoglycemic activity, in Taiwan folk, Momordica plants, for example: mountain bitter gourd is often used to treat diabetes.

In the current extraction technology, there are various operation modes in the extraction of natural substances, such as fractionation, precipitation, sublimation, impurity removal and chromatography, etc., and the use of solvent classification is more And water extraction, these two ways can be regarded as The basic method for the extraction of polar and non-polar substances is simple in steps and relatively low in cost. The disadvantage is that the purity of the extracted material is very low, and it is not easy to extract the active ingredient; the supercritical carbon dioxide extraction technology utilizes the solubility of the supercritical fluid. The relationship between density and pressure, the extraction of the supercritical fluid by the influence of pressure and temperature, and the use of an auxiliary solvent such as ethanol to contact the supercritical fluid with the substance to be separated under supercritical conditions to make it selective. The components with the same polarity and boiling point are sequentially extracted, and the extract obtained corresponding to each pressure range cannot be a single component, and the mixed components can be controlled under the optimal conditions, and then the supercritical pressure is obtained by means of decompression and temperature rise. The fluid becomes an ordinary gas, and the extracted material is precipitated, thereby achieving the purpose of separation and purification, and has the ability to simultaneously extract polar and non-polar substances. Carbon dioxide is a solvent that does not cause residual organic solvents, and is an effective and good extraction method, but Its low output and high production cost are difficult in the industry Line mass production scale.

In view of the above-mentioned shortcomings, the inventor believes that it has the urgent need to make corrections, and that it has been engaged in the design and manufacture of related products for many years, and has always adhered to the excellent design concept, and researched and created the above disadvantages. After continuous efforts, the extraction technology of the extract of Momordica charantia L. with the treatment of diabetes is finally introduced, which improves the efficacy of the product by correcting the excellent product structure.

The main purpose of the extraction technique of the extract of Momordica charantia L. with the treatment of diabetes in the present invention is to provide a bitter melon extraction technique which can effectively reduce the cost and improve the extraction rate.

In order to achieve the purpose of the foregoing disclosure, the present invention has an extraction technique of an extract of Momordica charantia L. for treating diabetes, and the extraction process mainly comprises pretreatment and extraction of bitter gourd, which is The treatment mainly consists of treating the bitter gourd fruit into a powder form, and the bitter gourd extract is mainly composed of a step of putting into a circulation tank, supersonic concentrated extraction and extracting the extract, and the input circulation tank is used to put the treated bitter gourd powder into an extraction. In the circulation tank of the machine, and performing ultrasonic concentrated extraction, the ultrasonic concentrated extraction system concentrates the ultrasonic waves on a predetermined position of the circulation groove, so that the bitter gourd powder can be shattered by the concentrated ultrasonic waves at the position, and the extraction is performed. After the end, the extract can be taken out. The ultrasonic wave is used to focus and illuminate the high-energy bubbles, and the cell wall of the bitter gourd is shaken in an instant, so that the bitter gourd nutrient is completely released and completely extracted, and the whole process is controlled at a low temperature. Under the condition, it overcomes the shortcomings of traditional extraction methods for high nutrient destructiveness, and replaces supercritical extraction in aromatic component extraction to solve the bottleneck of non-polar odor release problem faced by supercritical extraction, and uses water as medium, not There will be problems with solvent residue, which can also reduce production costs and reduce waste costs, so as to effectively increase the extraction rate in animal mode. Assessment, the results are also shown to have significantly improved glucose tolerance, improve insulin sensitivity, etc., can become a real person in the value of health food development.

1‧‧‧Pretreatment

2‧‧‧Bitter gourd extraction

21‧‧‧Into the circulation slot

22‧‧‧Ultrasonic concentrated extraction

23‧‧‧Remove the extract

3‧‧‧ extraction machine

31‧‧‧Circular trough

311‧‧‧

32‧‧‧concentration slot

33‧‧‧Supersonic

34‧‧‧cooler

First: Schematic diagram of the process of extracting bitter gourd of the present invention

Second: Schematic diagram of the extraction machine of the present invention

Fig. 3 is a graph showing changes in body weight during induction and administration of high lipid feed in mice subjected to animal model tests of the present invention

Figure 4: Oral glucose tolerance test for the fourth week of administration of the animal model in the animal model of the present invention

Figure 5: Oral glucose tolerance test of the eighth week of administration of the animal model in the animal model of the present invention

Figure 6: The mice in the animal model test of the present invention are administered in a diabetic animal model. The fourth week of insulin tolerance test

Figure 7: Insulin tolerance test of the eighth week of administration of the animal model in the animal model of the present invention

Figure 8: Data of serum biochemical values in the animal model test of the present invention, and the total cholesterol content (TC) in the serum in the fourth week

Ninth figure: The data of the serum biochemical value of the mice in the animal model test of the present invention, and the total cholesterol content (TC) in the serum in the eighth week

The tenth figure: the data of the biochemical value of the mice in the animal model test of the present invention, and the content of the triglyceride content (TG) in the serum in the fourth week

Figure 11: The data of the biochemical value of the animal in the animal model test of the present invention, and the content of the triglyceride content (TG) in the serum in the eighth week

Twelfth figure: The data of the biochemical value of the mouse in the animal model test of the present invention, and the LDL/HEL ratio in the serum in the fourth week

Thirteenth figure: The data of the biochemical value of the mouse in the animal model test of the present invention, and the LDL/HDL ratio in the serum in the eighth week

The invention relates to an extraction technique of an extract of Momordica charantia L. with diabetes treatment, [see the first figure]. The process mainly comprises pretreatment (1) and bitter gourd extraction (2): pretreatment (1), mainly The purchased bitter gourd fruit is processed into a powder form and becomes a bitter gourd powder, thereby entering the extraction process, and the bitter gourd fruit is a Momordica plant, for example, Momordica charantia var. abbreviata. .) or Momordica charantia Linn. The bitter gourd extract (2) is mainly composed of a step of introducing a circulation tank (21), ultrasonically extracting (22) and extracting an extract (23), and the input circulation tank (21) is used to input the treated bitter gourd powder. In a circulating tank, and performing ultrasonic concentrated extraction (22), the ultrasonic concentrated extraction (22) concentrates the ultrasonic waves on a predetermined position of the circulation groove, so that the bitter melon powder can be concentrated in the ultrasonic vibration at the position. Broken, and after the extraction is completed, the extract (23) can be taken out to complete the extraction procedure.

The invention relates to an extraction technique of the extract of Momordica charantia L. for treating diabetes, [please refer to the first figure and the second figure together] The process of the bitter gourd extraction (2) can be carried out by an extraction machine (3), wherein The extracting machine (3) mainly has a circulation tank (31), and the circulation tank (31) contains an extraction solution, and a pump (311) can be used to circulate the extraction solution in the circulation tank (31). A funnel-shaped concentration groove (32) is connected to one side of the circulation groove (31), and the concentrated groove (32) can be filled with pure water as a medium for transmitting ultrasonic waves, and the tip end of the concentration groove (32) and the circulation groove (31) Connected, and corresponding to the other end of the tip, an ultrasonic device (33) is provided, by which the bitter gourd powder is introduced into the circulation tank (31), and the bitter melon powder can be placed in the circulation tank (31) by the pump (311). Circulating flow, and the ultrasonic (33) can emit ultrasonic waves to the concentrating groove (32), and the ultrasonic wave can be concentrated at the tip end of the concentrating groove (32), and corresponding to the inside of the circulating groove (31) The bitter gourd powder is shattered by ultrasonic waves, and the extract is obtained and the extract is obtained.

The invention has the extraction technology of the extract of Momordica charantia L. for treating diabetes, [please refer to the first figure and the second figure together]. The present embodiment is described by the mountain bitter gourd. The detailed extraction process is: firstly, the selected one is selected. The fruit of Momordica charantia L. is placed at a temperature of 23±5°C, and is sprayed into powder to become a powder of Momordica charantia L.. After the pretreatment (1), the bitter gourd powder can be put into the circulation tank (31). In this embodiment, the The capacity of the circulation tank (31) is 300L, the input of the balsam pear powder is 100kg, and in the circulation tank (31), pure water is used as the extraction medium, and the pump is started at a temperature of 23±5°C. , the ultrasonic (33) and the chiller (34) are subjected to ultrasonic concentrated extraction (22), and the ultrasonic concentrated extraction (22) concentrates the ultrasonic energy in the circulation by the concentrating groove (32). At a predetermined position of the groove (31), the bitter gourd powder can be shattered by the ultrasonic energy at the position, and the ultrasonicizer (34) is cooled because the ultrasonic vibration causes the temperature to rise. The temperature is extracted after 2 hours of extraction, and the extract (23) can be taken out to complete the extraction process, and the extracted extract has the components of protein, saponin, polysaccharide and the like.

The above-mentioned mountain bitter gourd ultrasonic extract is further compared with the extract extracted by other methods, and the experimental animal model is executed as follows:

(1) Weight monitoring: Male mice with sex (6-week-old) were given a high-fat diet with a fat content of 40% for eight weeks, followed by intraperitoneal injection of NA 200 mg/Kg, and 15 minutes later, intraperitoneal injection of STZ. 50mg/Kg for 7 consecutive days, so that the non-fasting blood glucose level exceeded 200mg/dl, then group experiments, each group of 6: each group is a not administered (Normal), b control group (Control, normal) Mouse), c hypoglycemic agent (Rosiglitazone 10mg/kg), d hypoglycemic agent + the bitter gourd extract of the invention (Rosiglitazone 10mg/kg + BM RTSES 250mg/kg), e mountain bitter gourd ethyl acetate extract (BM EtOH 500mg/kg), f. balsam pear n-butanol layer extract (BM BuOH 500mg/kg), g mountain bitter gourd aqueous layer extract (BM Water 500mg/kg), h the present invention bitter gourd extract concentrate (BM RTSES (condensed) 500 mg/kg), i the bitter melon extract (BM RTSES 500 mg/kg) of the present invention, each group was fed orally once a day for eight weeks. The environment is 12/12 Light and dark cycle change, temperature control 24±2°C, food and water are supplied in an unlimited amount, and body weight is measured once a week, [see the third figure]. Experimental results: During the first to third weeks of the experiment, each group has some weight The decline was presumed to be the initial adaptation period of the drug feeding action. After the third week, the weight of each group began to rise gradually. During the five to seven weeks, the weight of the experimental group of the mountain bitter gourd was between 26 and 29 g. Both were lower than the positive and negative control groups.

(B) Oral Glucose Tolerance Test: The mice were measured for abnormal glucose tolerance before administration and two weeks and four weeks after administration. After the mice were fasted for 10 hours, the fasting blood glucose of the mice was measured by tail blood sampling using a blood glucose meter MAJOR II, followed by a group experiment, each of which was 6: each group was a not administered (Normal) , b orally administered 3mg/Kg glucose to the control group (Control), c hypoglycemic drug (Rosiglitazone 10mg/kg), d hypoglycemic agent + the bitter gourd extract of the invention (Rosiglitazone 10mg/kg + BM RTSES 250mg/kg ), e mountain bitter gourd ethyl acetate extract (BM EtOH 500mg/kg), f mountain bitter melon butanol layer extract (BM BuOH 500mg/kg), g mountain bitter gourd aqueous layer extract (BM Water 500mg/kg) , h BM RTSES (condensed 500mg / kg), i the bitter gourd extract (BM RTSES 500mg / kg), measured at 15, 45, 95, 135 minutes after glucose administration Blood glucose level, observation curve change, [see the fourth and fifth figures] The experimental results: (1) In the fourth week data, the glucose tolerance recovered most in the extract experimental group compared with the positive control group. It is an extract of e. sinensis acetate ethyl acetate layer, followed by i the bitter gourd extract of the present invention; (2) c hypoglycemic drug and d Bitter melon extract + glucose medicament of the present invention In the performance of glucose tolerance, the addition of the bitter gourd extract of the present invention does not significantly improve glucose tolerance; (3) i the sugar concentration area of the bitter gourd extract of the present invention per unit time is significantly lower than that of the positive control group, and Compared with other various extraction methods, it is only slightly higher than the extract of e. sinensis acetate ethyl sulphate, but it has a significant improvement compared with the two extracts of b. balsam, n-butanol layer extract and g. Sugar tolerance; (4) In the eighth week data, c hypoglycemic drugs and d hypoglycemic drugs + the bitter gourd extract of the present invention in glucose tolerance, d hypoglycemic drugs + the bitter gourd extract of the present invention is not significantly improved , similar to the results of the fourth week.

(C), Insulin Tolerance Test: The mice were measured for insulin resistance before administration and at two and four weeks after administration. After the mice were fasted for 10 hours, the fasting blood glucose of the mice was measured by tail blood sampling using a blood glucose meter MAJORII, followed by a group experiment, and each group consisted of 6: each group was a not administered (Normal), b intraperitoneal injection of insulin 0.5U / Kg to the control group (Control), c hypoglycemic drugs (Rosiglitazone 10mg / kg), d hypoglycemic drugs + the present bitter melon extract (Rosiglitazone 10mg / kg + BM RTSES 250mg / kg), e mountain bitter gourd Ethyl acetate layer extract (BM EtOH 500mg/kg), f. bitter gourd n-butanol layer extract (BM BuOH 500mg/kg), g. bitter gourd aqueous layer extract (BM Water 500mg/kg), h invented bitter gourd The extract was concentrated (BM RTSES (condensed) 500 mg/kg), i the bitter melon extract (BM RTSES 500 mg/kg), and the blood glucose level was measured at the time points of 15, 45, 95, and 135 minutes after the injection of insulin, and the curve was observed. Change, in order to know the difference in sensitivity of each mouse to insulin; if the blood glucose concentration does not decrease after the injection of insulin, it means that the cell can not receive the signal carried by insulin, [see Figure 6 and Figure 7] The experimental results: (1) Compared with the control group, the experimental data of the fourth week was the most obvious increase in insulin sensitivity. The extract of bitter gourd extract was concentrated in the present invention, followed by the hypoglycemic drug + the bitter gourd extract of the present invention, and the other groups showed no significant improvement. Insulin sensitivity effect; (2) comparing c hypoglycemic drugs and d hypoglycemic drugs + the bitter gourd extract of the invention, d hypoglycemic drugs + the bitter gourd extract of the invention has a significant increase in insulin sensitivity; (3) the eighth week In the insulin tolerance test, e-Moss bitter gourd ethyl acetate extract has a higher insulin sensitivity, and other groups of insulin sensitization are less obvious; (4) c hypoglycemic drugs and d hypoglycemic drugs + In the data of insulin tolerance of the eighth week of the bitter gourd extract of the present invention, the insulin sensitization degree was significantly increased in the control group, but there was no significant difference in the insulin sensitization degree between the two groups.

Next, the serum biochemical value test was performed on the experimental rats, and the total cholesterol content (TC) in the serum, [see the eighth figure], the results of the fourth week of the experiment: compared with the b control group, the present invention bitter gourd extract Concentrated, the lowest content, proved to be effective in reducing total cholesterol in the blood; [Please refer to the ninth figure] The results of the eighth week of the experiment: Compared with the b control group, i the bitter gourd extract of the present invention has the lowest content, which proves to be effective in reducing The total cholesterol in the blood.

Serum biochemical value test, serum triglyceride content (TG), [see the tenth figure] its fourth week of experimental results: compared with the b control group, c hypoglycemic drugs, d hypoglycemic drugs + the present invention The bitter gourd extract, the e-bitter melon ethyl acetate extract, the f-bitter melon butanol layer extract, and the begian melon extract of the present invention all effectively reduce the triglyceride content in the blood; [see the eleventh figure 〕 The results of the eighth week of the experiment: the same as the fourth week, c hypoglycemic drugs, d hypoglycemic drugs + the bitter gourd extract of the invention, e mountain bitter gourd ethyl acetate extract, f mountain bitter gourd n-butanol layer extraction The i., the bitter gourd extract of the present invention can effectively reduce the triglyceride content in the blood.

Serum biochemical value test, serum LDL/HDL ratio, [see Figure 12] The results of the fourth week of the experiment: compared with the b control group, the ratio did not significantly decrease; [see the thirteenth map 〕 The results of the eighth week of the experiment: compared with the b control group, e mountain bitter gourd ethyl acetate layer extract, g mountain bitter gourd aqueous layer extract, i the present bitter gourd extract ratio has a reduced effect.

The invention has the advantages that the extracting technology of the extract of Momordica charantia L. with the treatment of diabetes has the advantage that the bitter gourd uses the ultrasonic concentrated extraction method, uses the ultrasonic wave, focuses the high energy bubbles generated by the concentrated oscillation, and instantly shakes the bitter gourd cell wall. Broken, so that the bitter gourd nutrients are completely released for complete extraction, and the whole process is controlled under low temperature conditions, which overcomes the shortcomings of traditional extraction methods for high nutrient destructiveness, and replaces supercritical extraction in aromatic component extraction to solve supercritical extraction. The non-polar odor that is faced with the problem bottleneck is released, and water is used as the medium, there is no problem of solvent residue, the production cost can be reduced, the waste cost can be reduced, and the extraction rate can be effectively improved. In addition, the experimental results are comprehensively observed. The ethyl acetate extract has excellent performance, and the bitter gourd extract of the present invention has certain remarkable effects in various animal experiments, even surpassing the ethyl acetate extracting group of the mountain bitter gourd, and the ethyl acetate is finally an organic solvent, still on the human body. It has a certain hazard, but the bitter gourd extract of the present invention uses water as an extraction medium and has no Solvent residue doubts, therefore, have to develop in bitter melon extract considerable advantage, other extraction methods can not match, can become a real value in health food development.

In summary, it is understood that the present invention is novel, and the present invention is not found in any publication, and is in compliance with the provisions of Articles 21 and 22 of the Patent Law.

Only the above description is only a preferred embodiment of the present invention, when It is intended to limit the scope of the invention. That is, the equivalent changes and modifications made by the invention in accordance with the scope of the invention are still within the scope of the invention.

Claims (1)

The invention relates to an extraction technique of a mountain bitter gourd extract for treating diabetes, which mainly comprises: pretreatment, which mainly processes the purchased bitter gourd fruit into a powder form, and becomes a bitter gourd powder, wherein the bitter gourd fruit uses the mountain bitter gourd, and the environment The temperature is at 23±5°C, and then the extraction process can be entered; the bitter gourd extraction is mainly composed of steps of inputting a circulation tank, supersonic concentrated extraction and extracting the extract, and the input circulation tank system will be treated. 100kg of bitter gourd powder is put into a circulating tank with a capacity of 300L in an extractor, and ultrasonic concentrated extraction is performed at an ambient temperature of 23±5° C., and pure water is used as an extraction medium in the circulation tank, and the ultrasonic concentration is concentrated. The extraction system concentrates the ultrasonic waves at a predetermined position of the circulation tank, so that the bitter gourd powder can be shattered by the concentrated ultrasonic waves at the position, and the extract is taken out after the end of the extraction for 2 hours.