TWI247894B - Trackable optical discs with concurrently readable analyte material - Google Patents

Abstract

Design, manufacture and use of optical discs that permit the concurrent and discriminable acquisition of signals from both operational structures and nonoperational structures is presented. The disc geometries and tracking schemes permit such discs to be read in, and data encoded by nonoperational structures reported by, standard (or minimally-modified) optical disc readers. Single data layer first and second surface discs are described, as are multiple data layer discs. Use of the discs in analyte-specific assay is presented.

Description

Printed by the Ministry of Economic Affairs, Intellectual Property Office, Staff and Consumers Co., Ltd. 1247894 A7 _B7_ V. INSTRUCTIONS (1) Scope of the Invention The present invention is attributed to the design and manufacture of optical discs and the use of prior disc readers and readers. In particular, the present invention relates to the design, manufacture, and use of optical discs that permit simultaneous verification of signal elements by optical disc non-operational structures, such as specific analytes, and simultaneous and identifiable capture signals from optical disc operable structures such as tracking structures. BACKGROUND OF THE INVENTION Scanning confocal laser microscopy (SCLM) has revolutionized life science imaging over the past decade. In scanning confocal laser microscopy, laser light is scanned across the sample at an accurately selected focal plane. The laser light from the opposite side of the sample is collected, and the laser light reflected outside the selected focal plane is removed, and the flash is removed, resulting in clear image of the segment from full thickness, non-fixed tissue and cells. In addition, the reworked spatial density of the computer-driven scanning process allows for the correct spatial registration of the separately expanded segment images, allowing for the reconstruction of the three-dimensional image by overlapping the segment images. Wiesendanger, Scanning Probe Microscopy and Spectroscopy: Methods and Applications, Cambridge University Press (July 1995); Cullander, J. Investig. Dermatol· Symp. Proc. 3:166 - 171 (1998) ; Paddock, Proc. Soc. Exp Biol· Med. 213:24-31 (1996); Ockleford, J. Pathol. 176:1-2 (1995); Laurent et al, Biol. Cell. 80:229-240 (1994) o Laser-fired The use of light dyes and proteins for specific ligand probes has allowed scanning laser microscopy to be adapted to standard cell and tissue imaging to become widely variable assays. Therefore, laser scanning cell meters have proven to be particularly advantageous for fluorescence-based cytometry to determine cell cycle events. -4- Long-term application of China National Standard (CNS) A4 specification (210 X 297 public β --------------------- order -------- -Line (please read the notes on the back and fill out this page) 1247894 A7 B7 V. Description of invention (2)

Juan et al, Methods Mol Biol, 91:67_75 (1996); Juan et al, Cell Biol. 2:261-273 (1998); Juan et al, Cell Biol. 2:341-350 (1998); Catch et al. Human, Cytometry 34:36-38 (1998); Luther et al, Microscopy & Micro analysis 3: 235-236 (1997).

The "Genome Reporting Matrix" for scanning laser microscopy is described in U.S. Patent No. 5,549,588 to Ashby et al. In these genome reporting matrices, the elements of the spatially addressable matrix contain cells in which the common fluorescent report is driven by different translational regulatory elements. The intensity of the fluorescent signal captured during the scan identifies the level of the gene driven by each of the spatially identifiable translational regulatory elements. Scanning laser microscopy has also been adapted to scan nucleic acid microarrays based on ruthenium wafers, Lashkari et al., Proc. Natl. Acad. Sci. USA 94:13057-62 (1997); DeRisi et al., Science, 278:680-86 (1997); Wodicka et al, Nature Biotechnology, 15: 1359-67 (1997); Measured to measure ion flux in cells, Schild, Cell. Calcium 19:281-296 (1996) Turner et al, J. Investig. Dermatol· Symp. Proc. 3: 136-142 (1998); and measurement of subcellular distribution adapted to various cellular components, Takubo et al., Haematologica 82: 643-647 (1997) ° ° Each of these SCLM applications requires special parts for computer controlled optics. There is therefore a need in the art to have inexpensive general devices that allow computer-driven confocal laser scanning of microscopic samples. The minimum mechanical requirements for such devices, laser, focus and detection optics, precision scanning devices and computer interfaces - all can be applied to the National Standard (CNS) A4 specification on standard optical disc readers or on the scale of this paper. (21〇X 297 public) (Please read the note on the back and fill out this page) - II - II ^ · 11111111 . Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed A7 1247894 __ B7_ V. Invention description (3 (Please read the notes on the back and fill out this page) Found in the writer. Optical disc readers and readers, such as CDs and DVDs, focus light from a solid laser onto the surface of the rotating optical disc and scan the optical disc to detect digitally encoded information in a sub-micron structure of the spatially addressable pattern. The adaptation of optical discs and optical disc readers to laser scanning applications will present significant advantages over existing methods. The main advantages between these advantages are wide applicability and low cost. The world's CD and DVD-ROM reader mounting bases are currently estimated at approximately 300 million units and are expected to grow to more than 500 million units over the next five years. Optical Publishing Industry Assessment, 9th edition (Infotech, Inc., Woodstock, Vermont) (1998). The device is versatile, inexpensive and reliable. Other advantages of the use of optical discs for the detection and characterization of optical discs printed by the Intellectual Property Office of the Ministry of Economic Affairs, WO 96/09548 (Gordon), EP A 392475 (Idemitsu), EP A 417 305 (Idemitsu), EP A Discussion is in 504432 (Idemitsu), WO 98/28623 (Gamera) and WO 98/12559 (Demers), all of which are incorporated herein by reference. Other advantages are co-owned and pending US patent application number 08/888,935, July 7, 1997 application; 09/064,636, April 21, 1998 application; 09/120,049 No., the application of which is hereby incorporated by reference in its entirety in its entirety in the the the the the the the the the the the the the the the However, while optical inter-disc machines have the mechanical prerequisites for effective confocal laser microscopy, the operational requirements of existing optical disc readers present significant obstacles to the successful detection and characterization of microstructures disposed on the surface of optical discs. At least 4 operating area requirements must be met for the disc to properly read and -6- this & Zhang scale applicable Chinese National Standard (CNS) A4 specification (210 X 297 mm) """"&quot " 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 5, Invention Description (4 Decoding the data presented on the CD: The reader must be correctly focused on the disc surface that encodes the greedy material, must control the optics The radial position of the pickup, the position of the tangent of the optical pickup must be controlled, and the number of revolutions of the optical disc must be controlled. The most common optical disc system uses optical media itself to meet at least some of these needs. Therefore, in a typical suppressed c D, the disc The board is embossed with a spiral track consisting of a series of embossed holes, and the resulting signal is used by the disc reader to maintain proper focus and tracking. In CD_R, the data encoded by the user is coded. The markup provides the necessary look-up structure for sequential reading. More generally, in the standards of existing optical disk standards, the structure used to encode data is simultaneously made. In order to provide the operating signals required by the reader to control its operation, although efficient, such standards do not provide provisions for extracting data from non-operational structures configured on the optical disc. For example, because the tracking structure is forced to be embedded in In the information layer of the disc, the structure closest to the surface of the laser applied to the disc may hinder the detection of such an operational structure and thus hinder the correct operation of the inter-reader. Even such a non-operational structure may be placed on the disc. Operating the structural focal plane sufficiently external to prevent simultaneous and identifiable detection by the reader optical pickup. One solution to this problem is to make a non-standard transmission. One proposed transmission utilizes two optical pickups, One detects the tracking information and the other detects the surface structure, EP A 392475 (Idemitsu), EP A 417 305 (Idemitsu), EP A 504432 (Idemitsu) and EP A 417 305 (Idemitsu). The correction assumes that the main advantage of using the inter-disc reader for laser micro-detection is the general distribution of such devices. -7- This paper scale applies to China Standard (CNS) A4 specification (210 X 297 mm) " ' .1-----------------Book --------- (Please read the back first Note on this page) 1247894

V. INSTRUCTIONS (5)· INTELLIGENT INTELLECTUAL PROPERTY SECTOR Employees' Consumer Cooperatives Printed in the related art of optical discs, there is a need to allow a standard disc reader/per unit to be placed on a disc by a non-operating structure such as a disc The 2 elements simultaneously and arbitrarily capture signals, and the signals are generated by a disc operating structure such as a tracking structure. SUMMARY OF THE INVENTION The present invention solves the related problems of the related art by providing an optical disc, a method of manufacturing the same, and a method of reading the optical disc, wherein the optical disc allows the inter-disc reader/writer to be configured on a disc by a non-operational structure such as a disc. The particular analyte signal element on it simultaneously and identifiably captures the signal, and the signal is generated by a disc manipulation structure such as a seek structure. The present invention is based in part on new discoveries that the non-operating structure can be confocal with the disc operating structure, as well as the laser closest to the previously applied reflective layer, thus addressing the optical problem of maintaining the focus on both the operative and non-operating structures. This geometry is achieved by fabricating a new optical disc, wherein the imaged surface of the complete substrate of the optical disc (that is, the surface of the substrate that contributes to the information layer of the optical disc) appears as the closest surface to the laser of the substrate ("inverted disc," Because the radius of the beam on the operating focal plane is substantially smaller than the radius of the beam on the first surface of the disc', these inverted discs make it possible to use sub-micron inoperative operation in a new way. Such a disc can be utilized The conventional master is manufactured as a stamper, thereby producing a forward image inverted disc having an operational structure presented in a positive relief. The second manufacturing process, in which a parent or a sub-sheet is used as a stamper, can also be utilized. In the second manufacturing process, the main film has an image opposite to the conventional one; the image is inverted to the image with a negative embossing operation -8 - the paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) ) -------IT---------^ (Please read the notes on the back and fill out this page) 1247894 A7 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative System and invention description (6 structure. Therefore, 'in preparation for going to AW, »_ |』 f signin', the present invention presents a substrate comprising a substrate having a patterned surface deducted from the two sides and the outer surface, The patterned surface of the substrate facilitates a first-disc information layer, wherein when the volume A 1 _ ? substrate substrate patterned surface appears as the laser closest to the outer surface of the neck, the information layer includes an operation surface that can be searched by the optical disk drive. When the invention is manufactured, the operation structure of the inverted optical disc can be configured to be an embossed forward image. In this method, the operation of the optical disc can be configured in a track-and-track manner. In the information layer plane, the information layer is at the farthest end of the additional surface of the substrate. ^ When the processing of the moon is processed, the operating structure of the inverted optical disk can be configured as a negative relief front image. In this orientation, The operating structure of the optical disc is configurably disposed in the plane of the information layer, the information layer being closest to the additional surface of the substrate. In a specific embodiment, the inverted optical disc further includes at least one non-operating structure, the Μ structure and the operating structure ( particular The search may be read at the same time. In particular embodiments of the specific embodiments, the non-operational structure may be a specific analyte signal element, and a disc-operated structure confocal configuration. In another feature, the present invention provides A method of manufacturing such an inverted optical disc. Thus, the present invention provides a method for fabricating a forward image, a embossed inverted optical disc. The optical disc substrate has a track-searchable structure, the method comprising: forming a track-reproducible operation a stamper of the structure having an equal image orientation and relief of the standard master; and thereafter forming the disc substrate directly from the stamper. In a second method, the present invention provides a method of fabricating a front image, a negative relief inverted disc The disc substrate has a track-operating structure, 9 - the paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) -l· —------------- --Book --------- line (please read the note on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed 1247994 A7 __;_ B7___ V. Invention description (7) Method includes: shape Forming the opposite main film, forming a stamp having an image orientation opposite to the opposite main sheet and embossing; and thereafter forming the optical disc substrate directly from the stamper. In another embodiment, one of the aforementioned new processes can be performed using a CD-R or CD-RW master (or vice versa) and the mold is a DVD face mask. Such newly formed optical discs and optical discs cast in CD molds have a reduced depth. The present invention also provides a method for fabricating an inverted optical disc that utilizes an inverted optical disc substrate to accommodate a track-readable reading of a non-operational structure. In this first particular embodiment, the present invention provides for fabricating a rewritable optical disc having a simultaneously readable non-operational structure, the method comprising: applying a reflective layer to a patterned surface of the inverted optical disc substrate, and a configuration and The reflective layer has a confocal and non-operating structure with a laser approaching. The child inverted optical disk substrate can be prepared by any of the foregoing. For the forward image/positive embossed inverted disc or the forward/negative embossed inverted optical disc of the present invention, there is no transparent substrate layer for the laser to approach the optical disc communication layer. Therefore, an additional device is required to change the length of the optical path, thus enhancing the focus of the incident laser on the surface of the reflective layer of the optical disc. This can be accomplished by attaching a non-integral or integral laser refractive member to the cover of the inverted disc laser access side to create the optical disc assembly. The cover acts as a refraction and thus further focuses the incident beam onto the disc operating plane. Accordingly, in another feature, the present invention provides a disc assembly having a readable non-operational structure, the assembly comprising: an inverted disc and a cover, wherein the panel further focuses the incident beam onto the disc information layer. In some specific ____·10- this paper scale applies Chinese National Standard (CNS) A4 specification (210 X 297 public f) ^99-------^--------- (please read first Precautions on the back page Fill in this page) 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed V. Inventive Note (8) In the embodiment, the cover is not integrated into the optical disc and can be attached thereto. In other embodiments, the cover is integrated into the optical disc. In some embodiments, the cover is essentially composed of plastic, consists essentially of glass, or consists essentially of pieces. The present invention provides other ways of achieving a non-operating mode confocal configuration. Thus, in another feature, the image of the optical disc operating structure, particularly the image of the track searchable structure, is projected onto the plane confocal with the non-operating structure. In a preferred embodiment, a reflected hologram is used. The advantages of the hologram include the physical separation of the operational structure and the non-operational structure on the optical disc. An additional way of separating such entities is to utilize multi-layer DVD technology. Accordingly, in another feature, the present invention provides a disc having a readable non-operating structure, including a reflective layer; an additional reflective layer; and a non-operational structure, wherein the reflective layer or the additional reflective layer has an inter-disc reader The structure of the search, and wherein the non-operational structure and the searchable structure are readable. In a typical embodiment compatible with the DVD standard, one of the reflective layers is semi-reflective, and the non-operative structure and the reflective layer or semi-reflective layer are confocal. While the new approach described above solves the optical problem of establishing focus on both the operational and non-operational structures, they do not themselves require simultaneous detection of the structure. First, these approaches do not need to address the problem of maintaining tracking, focusing, and synchronization (speed control) when presenting such a confocal configuration non-operational structure. Second, the non-operational configuration of the confocal configuration with the operational aspect (e. g., the hole) may cause insufficient signal to be distinguished by the operational signal to prevent such discrimination. This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm -------^--------- (please read the note on the back? Please fill out this page again) Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 1244894 A7 Factory - _____ Β 7 V. Invention Description (9) The present invention further relies on the discovery of electrical signals generated during reading with new information content that is more informative than previously known. That is, the electrical signal is designed to provide two specific categories of information - the signal is required for the operation of the disc and the asset is digitally encoded on the disc - in fact, it can be queried and measured in a variety of ways to reveal relevant The optical entity structure has not been previously developed with extensive information. Furthermore, the present invention actually illustrates that the required operational components of these electrical signals can be surely removed from the 'thus releasing the remaining ## component for the non-operational measurement without interfering with success. Driving operation. Therefore, in another feature, the present invention provides a separation of the portion ## designed to allow operation of the optical disc, and in particular, a portion of the signal required for tracking the optical disc is used. Reporting the partial signal separation of the non-operational structure reactions on the optical disc. In a series of exemplary embodiments, the optical disc is presented to allow the tracking signal to be separated by the non-operating signal frequency of the HF (RF, Four Columns) signal; The situation allows the HF signal to be used to report information that is configured on the non-operational structure. This purposeful separation of the HF signal's seek signal is to use the seek design to % into 'only in the HF signal (low frequency signal generation, the seek The rail design is intended to be ignored by the existing disc standard during disc reading, which is formulated in the writable and rewritable standards in CDs and DVDs. Associated with the inverted discs of the present invention are these late disc geometries. A purposeful, simultaneous and identifiable detection of a non-operating structure on an inverted optical disc. The present invention thus provides an inverted optical disc as described above, wherein the operational structure includes a wobble track. If the manufacturing process is processed, the wobble,纹道" with positive float-U------------------- order--------- line (please read the phonetic on the back? Page) -12- 1247894 Ministry of Economic Affairs Intellectual Property Officer Workers' consumption cooperatives print A7 V. Invention description (1〇) $ horse forward image (processing 1} or negative embossing as forward image. Late t to strengthen non-# structure such as specific analyte signal element The center is above the tracking structure during the capture period. The confocal configuration of the disc information layer and the non-operational structure closest to the Thunder J are simultaneously detectable with the wobble track and its signal can be identified by the wobble pattern. Example 2-3 shows the successful use of the single data layer, the forward image wobble track, and the embossed inverted optical disc of the present invention to (1) detect IgG in human blood by immunostaining, and (2) utilize The minimally corrected optical disc reader detects and characterizes human red blood cells captured on the surface of the disc by a specific immunological response. Example 7 actually illustrates the adaptation of the nucleic acid base assay to the detection principle thus described. BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects and advantages of the present invention will become apparent from the following detailed description of the accompanying drawings in which <RTIgt; Components, and wherein: Figure 1 shows a single-layer CD category disc and reader, and Figure 1a shows a side view of a reader optical pickup that is oriented between CDs, which is shown in a cross-sectional view. The laser light path is indicated by a line; and the image i B shows a side cross-sectional view of the same orientation of the optical disk with a large magnification; and FIG. 1c shows a perspective view of the CD-R optical disk with the wobbled track; Figure 2B shows the beam position of a typical 3-beam pickup with respect to the κ _ track, and a typical optical disc detector and associated electrons using 3-beam for tracking, focusing and reading; 'Figure 3 shows In the establishment of a single data layer disc 'Figure 3A shows the standard -13 - This paper scale applies _ home standard (CNS) A4 specifications (21 〇 X four 7 public) ^ ------ order ------- ----Line (please read the phonetic on the back? Please fill in this page again) A7

1247894 V. INSTRUCTION DESCRIPTION (11) Electron and substrate processing ~ Figure 3 B shows tf as the correction process of the present invention, and the master piece is used as a stamper, and the image is formed before the present invention. The embossed inverted disc; Figure 3 C shows the surrounding, Λ ^^ The traditional CD-R disc substrate punched in the country 3 以 is completed with the reflective layer and the protective layer, oriented relative to the interrogating laser. Figure 3〇 3B medium pressure P's to the image, the embossed inverted optical disc is completed with reflective layer and non-operational structure, and is oriented relative to the traditional interrogation laser; Figure 3E shows that this month is used in "quasi-electricity and substrate processing" Reverse image main film disc to obtain the previous image/negative embossed inverted disc. · Figure 4 shows the application of the air applied to the traditional single-layer ^ d _ category disc with side-by-side cross-surface display. The laser is closest to the first The structure of a surface, the actual description of the optical pickup of the reader and the interruption of the optical path produced by the optical pickup of the reader; Figure 5 actually illustrates one type of inversion by reversing the conventional category of discs, and Figure 5a And Figure 5B The side cross-sectional view actually illustrates the reversal of the solid structure, the laser distal structure causes the laser to approach and the laser approaches the structure to become the far end of the laser, while Figures 5C and 5d actually illustrate the reversed image of the tracking structure. Figure 6 is a side view of an optical pickup positioned to read a single layer, a first surface, a trackable specific analyte optical disc of the present invention, shown in a side cross-sectional view, with the laser optical path indicated by a line, 6A actually illustrates the focus before the focus lens is additionally added to the optical pickup, and FIG. 6B actually illustrates the change of the focus when the focus lens is additionally added to the optical pickup; FIG. 7 illustrates the molecular composition, which is established on the first surface of the present invention, The IgG-specific immunoassay on the reflective surface of a specific analyte CD can be tracked, while Figure 7 A-14 - This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) (please read the back first) Note: Please fill out this page) ώψ Order---------Line. Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1244894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed V. Explain that (12) shows the test grid before the sample is increased and Figure 7B shows the specific immune attachment of the latex spheres mediated by IgG in the human blood sample, followed by an increase in the human blood sample and thereafter by additional anti-IgG conjugate The latex sphere is "developed"; Figure 8 is a video image captured by a microscopic inspection of the light of a part of the IgG-specific first surface specific analysis wheel after application of human blood and antibody conjugated latex spheres; Figure 9 and Figure 10 is an atomic force microscopic (AFM) image of a single latex sphere that is attached to the first surface for specific immunity to the human-IgG-specific disc, using a slightly higher magnification than Figure 8, with the AFM observed during image capture. Summarizing the quantitative size; Figure 11 is an atomic force microscopic (AFM) image of a specific latex-attached body-IgG-specific disc-dispersing globule, and presented in the same AFM field, with AFM in the image capture period. Observe the quantitative size of the observation; Figure 1 2 shows the AFM image and the red blood cell (RBC) of the specific RBC-specific CD attached to the first surface. Summary of Sizes; Figure 13 is a graph showing the specific analyte perturbation of the HF (four-column sum) signal obtained by the optically-displaceable scan on the RBC of the first surface traceable human-RBC-specific disc by the optical disc reader. The digital oscilloscope tracks, the X-axis displays the time and the x-axis shows the four-column sum signal size; Figure 14-17 shows some of the separated RBCs that are attached to the first surface by the CD reader to the first surface steerable human-RBC-specific disc. The digital representation of the specific analyte perturbation of the HF (four-column sum) signal obtained on the searchable scan -15- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) l·---- -------Αν-------Book---------^^99 (Please read the notes on the back and fill out this page) 1247894 Ministry of Economic Affairs Intellectual Property Office Staff Cooperatives Printed A7 B7 V. Invention Description (13) Waveper tracking, X-axis shows time and γ-axis shows four-column sum signal size; Figure 18 is the overlap of most events taken by the same disc class, actually indicating some specific immune attachment Reproducibility of different rBC size and shape measurements to the disc; 19 is a side cross-sectional view of a single layer of a specific analyte optical disk that can be tracked by a non-integrated laser proximity, laser refractive cover; FIG. 20 shows a comparison of a single layer of a specific analyte optical disk of the present invention. In a preferred embodiment, the optical disc operating structure that reflects the holographic image reflects an image of the optical disc seek structure in a plane confocal with the non-operating structure disposed on the first surface of the optical disc; FIG. 2 shows a linear velocity according to the area. (ZCLV) Simplified upper side view of a standard constructed optical disc physical organization; Figure 22 shows an enlarged perspective view of one of the sectors of the ZCLV disc of Figure 21; Figure 23 shows the wobble pattern of a particular analyte disc sector disposed on the first surface of the ZCLV Figure 2 shows a side cross-sectional view of a typical double-layer DVD format disc; Figure 25 shows an enlarged side perspective view of a combinable dual-data layer specific analyte verification disc; Figure 26 A side cross-sectional view showing a specific embodiment of a two-layer specific analyte verification optical disc containing an internal wanted; Figure 2 7 shows a two-layer specific analyte verification light with an internal verification-reinforced structure Side cross-sectional view; Figure 2 8 Another aspect of the invention showing the double-layer specific analyte verification of the side of the optical disc - 16 - This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 public) --- ----Book---------Line (please read the note on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1244894 A7 _- B7 V. Invention Description (14) Figure 29 shows another embodiment of a two-layer specific analyte optical disc; Figure 30 shows a side cross-sectional view of a portion of the spherical signal emitted in the disc track, indicating various sizes; The data generated by the AFM inspection of the inner diameter of the single data layer of the present invention is further optimized for the first surface detection of FIG. 8 - FIG. 12 and the reported texture depth of approximately 1 〇〇 nm. Figure 32 is a representation of the AFM inspection of the outer diameter of a single data layer disc of the present invention. The optical disc is further optimized for first surface detection relative to Figures 8 - 12 and has a report of approximately approximately 1 〇 1 nm. Grain depth; Figure 3 3 is presented for stamping at 3 i and Fig. 3 2 measured optical disc, master, and inner diameter AFM inspection data; Figure 3 4 shows the disc used to stamp the optical discs measured in Fig. 31 and Fig. 3, the master , AFM inspection of the outer diameter of the data; Figure 35 is a perspective view of the polycarbonate laser refraction cover, and the use of the optical disc of Figure 31 and Figure 32 has produced the data shown in Figure 4; Figure 36 A specific analyte verification grid based on the nucleic acid base of the present invention is illustrated, wherein a single 2·8 μπι sphere is specifically attached to the surface of the disc for driving by nucleic acid sequence complementation; FIG. 37 presents a light microscopic of 3 discs. The image, each image is 2 magnifications, and is established using the verification geometry shown in Figure 36. Figure 3A shows that the nucleic acid sequence of the sphere is supplementally driven to attach to 20 teraflops (2〇&gt;&lt;丨〇七莫耳, target nucleic acid; Figure 37B shows that the nucleic acid sequence of the sphere is supplementally driven to attach to 2 tera billion of moor (20 χΐ〇Μ mol) target nucleic acid; Figure 37c shows the sphere ----- --Book --------- line (please read the notes on the back and fill out this page)

A -17 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1244894 Δ7 Α7 ___Β7 _ V. Invention Note (15) The nucleic acid sequence supplement drive is attached to 20 teraflops (20 χ 1 〇-21 摩尔) Target nucleic acid; Fig. 38 is a two-dimensional synthetic light microscopic image of 300 X magnification which is closest to the surface size of those optical discs measured by AFM in Fig. 31 and Fig. 32, and the 2·8 μιη sphere is electrostatic Attached to the metallized surface and substantially manually aligned along the track; Figure 39 is a higher magnification portion of the same disc as shown in Figure 38; Figure 40 is shown in the area of the disc shown through Figure 39 The electrical response of the single magnetic HF signal is reported; and Figure 41 presents the settings of the mold used in the manufacture of the optical disk described in Example 5, and is shown in Figures 31, 32, 37, 38 and 39. DETAILED DESCRIPTION OF THE INVENTION In order that the invention as described is fully understood, the following detailed description is set forth. In this description, the following terms are utilized. § As used herein, the term "radial" means the direction of advancement or retreat along the spiral of the track in the plane of at least one of the disc data encoding surfaces (i.e., the optically detectable surface of the disc information layer). The surface of the optical disc of the present invention may be an internal or external surface. When used thereby, the term "tangent," means in the plane of at least one of the disc-encoding surfaces, in the direction of the line drawn from the center of the disc to the outer circumference, inward or outward. The term "radial plane" is taken as the plane in which the disc can be tracked (i.e., spiral tracked) and is the plane of at least one of the disc data encoding surfaces. -------1--------- (Please read the notes on the back and fill out this page)

A7 1247894 B7 V. INSTRUCTIONS (16) When used herein, the term "non-operating structure" means any structure disposed on the surface of a disc or on a disc, when the disc is read by a disc reader, The structure can produce a signal to the dog, however the structural signal is not required (although advantageous) for the driving operation during reading. Non-operational structures include, for example, specific analyte signal elements, as explained below. When used herein, the term "specific analyte signal element" is taken to mean any non-operational structure that can be used to signal the presence of a particular analyte in a sample applied to the disc. The term thus includes, inter alia, that the signal element, including the beading and the cell, as exemplified, and the commonly-owned and co-owned U.S. Patent Application Serial No. 8/888,935, filed on July 7, 1997; 09/120,049, application on July 21, 1998; 09/183,842, application on October 30, 1998; 09/311,329, application on May 14, 1999; 60/134,368, May 14, 1999 Application; Siddiqi et al., "Methods and Apparatus for Physically Patterning Nonoperational Structures on an Optical Disc," US Provisional Patent Application No. 60/150.2873⁄4, August 23, 1999 application (Office file number BURST- 14P); Worthington et al., Methods and Apparatus for

Analyzing Operational and Nonoperational Data Acquired from Optical Discs, "US Application Serial No. 09/378.878, August 23, 1999 application (office file number BURST-15); and Worthington et al. "Methods and Apparatus for Optical Disc Data Acquisition Using Physical Synchronization Markers," US Provisional Patent Application No. 60/150.288#., August 23, 1999 application (office file number BURST_18P) for those signal elements; This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) (please read the notes on the back and fill out this page) -------tr---------Μ , Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperative, Ministry of Printing, Economy, Intellectual Property, Staff, Consumer Cooperative, Printed, 12,478, 489 A7 B7 V. INSTRUCTIONS (17) The documents in the application are hereby incorporated herein by reference. The term encompasses those structures that are detectable separately by an inter-disc reader and those that require additional components to be detectable. To provide a further discussion of the present invention. BACKGROUND OF THE INVENTION The projection structure of a conventional optical disc is briefly described in association with Fig. 1. Fig. 1A depicts an optical pickup (splicing assembly) 10 and a standard CD-class optical disc 1 1 ' and the optical path between the two components is indicated by a broken line. Figure 1b provides a side cross-sectional enlarged view of the optical disc 1 in the same orientation relative to the incident laser. For reference, Figure 1A and Figure 1B, the optical The pickup comprises a laser source 19, a lens 12-14' beam splitter 15, a quarter wave plate 20 and a detector 18. The laser source 19 is typically a laser diode that emits a laser beam via a beam of light. The splitter 15 is transmitted and then calibrated by the lens 12. The beam of sight is then reflected by the beam splitter 15 forward to the disc 11. The objective lens 13 focuses the laser beam on the disc 1 1 near the laser or the first surface The small spot on the laser beam is reflected by the surface of the disc reflection layer 114 (in the conventional CD-class, the second indication) and is rotated to the beam splitter 15 via the objective lens 13 and the quarter-wave plate 20. The beam splitter 15 Guided reflection of the laser beam via a lens 1 4, the lens will be focused on the detector 18. The astigmatism element 16 can be included between the beam splitter 15 and the detector 1 8 to introduce astigmatism in the reflected laser beam. As shown in more detail in Fig. 1B, the CD-class optical disc 11 includes a 3 physical layer: the laser is approached to the distal end of the laser as the transparent substrate 112, the reflective layer 114, and the protective layer 116. The total depth of each layer combination is negligibly 1.2 mm. The figure is not -20- This paper scale applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) ------------------- Order---- -----Line (please read the note on the back and then fill out this page) 1247894 Α7 — Β7 V. Invention Description (18) Proportion to draw. (Please read the note on the back and then fill out this page.) When measuring along the optical axis, the transparent substrate 112 forms the majority of the 1.2 mm thickness of a typical CD-class disc, and functions as an optical and structural function. These dual roles specify that the substrate 112 has a planar surface and a patterned surface. · While conventional optical discs are conventionally read, the planar surface appears as a laser close to the surface of the two surfaces of the substrate, and the patterned surface appears as a laser on the surface of the substrate. Distal surface. Relative to its optical function, the refractive nature of the transparent substrate 112 acts to shorten the optical path in the monochromatic system; basically, the transparent substrate 112 acts further to focus the incident laser onto the reflective layer 114: in a CD-class disc The laser is near the surface or the first surface, and the laser spot has a diameter of about 8 μm; the transparent substrate 112 further focuses the beam, and the reflective surface 114 of the second surface of the optical disk reaches about 1·7 μm. diameter. In a typical CD, the transparent substrate 112 is mainly composed of polycarbonate, and thus has a refractive index of j 55 . It will be apparent to those skilled in the art that materials other than polycarbonate can be used as long as the material thickness and refractive index provide sufficient assistance to the disc reader focusing system to determine focus on reflective layer n4. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumers' Cooperatives. The transparent substrate 112 also provides the main structural integration of the CD 11. The reflective layer 114 has a thickness of about 0.05 to 0.1 μm, and the protective layer 116 typically comprises a lacquer material that will harden when exposed to UV light and have a thickness between 10 and 3 micrometers. Therefore, the transparent substrate 112 constitutes the main physical layer of the optical disc, and is the only layer capable of transmitting the animal sufficiently rigid to the optical disc to retain the operating structure of the optical disc. This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (19) The substrate layer 112 is typically suppressed by a spiral track, The magnetism is spiraled from the innermost part of the disc to the outermost readable part of the disc. In a non-recordable disc, the track consists of a series of holes, each having a depth of about 1/4 of the wavelength of the light used to read the disc. The hole has a varying length, and the length and spacing of the hole encodes the data. As discussed further below, the standard CD-R recordable optical spy contains spiral tracks instead of holes. A partial cross-sectional view of such a wobble track 118 is shown graphically in the perspective view of Figure 1C. The reflective layer 114 has a thickness of approximately 〇·〇5 to 〇·ΐ microns, and typically includes a reflective metallic material such as aluminum, silver, gold or copper. Reflective layer U4 is typically applied to substrate 112 patterned surface via a magnetic % tube spray process. Another treatment, steaming, can be used, but much slower. Chemical wet "silver plating, processing (using silver, nickel or other metals) can also be used to form the reflective layer 114 on the patterned surface of the transparent substrate 112. The protective layer 116 typically comprises a shell lacquer material when exposed In the case of ultraviolet (uv) light, known as the "curing" process, the material will harden between 1 〇 and 3 〇 microns. The protective layer 116 acts to prevent the reflective layer 114 from scratching and oxidizing, and provides a label a convenient surface printed thereon. The protective layer U6 is typically applied to the transparent substrate 112 and the reflective layer 114 via spin coating treatment, so that when exposed to UV light, a small amount of material is sprayed onto the disc near the inner diameter of the reflective layer U4, Moreover, the optical disc rotates at a high speed, and the thin layer of material is covered on the surface of the optical disc. Thereafter, the optical disc is exposed to UV light, and the lacquer material is hardened. Although the physical layer is included, the optical disc 11 is also regarded as having an "information layer." , (also -22- This paper scale applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) ------- order--------- (please read the back Please fill out this page again) 1247894 A7 B7 V. Description of the Invention (2〇) "Data Layer" 115, at least the transparent substrate 112 and the reflective layer 114 are physically contributed to the layer. (Please read the notes on the back and fill out this page.) The “Information Layer” is defined by the second plane. For this purpose, 'plane A, the laser is closest to the radial plane and plane B is the farthest radial plane of the laser, and the 琢 operating structure extends to this plane. In standard c D or single-layer DVD, there is only a single information layer, and the optical disc is traditionally described as a single data layer (or "single layer,") disc. Various CD and DVD standards are expected to be insignificant. The depth (defined by the optical axis) is 1.2 mm and the radial diameter is 12 mm. The micro-insignificant diameter disc. Although the thickness is 1.2 mm, the senior standard of the disc technology is The Red Book ',), reissued as IEC 908, allows thicknesses of 1.1-1.5 mm (for all layer combinations). However, the reader can accommodate some additional changes' and the discs suitable for reading by CD and DVD drives can have a maximum of approximately 2.4 mm with a minimum of approximately insignificant 〇.8 mm depth 'better 舄1·〇· 1.4 mm, more preferably 1·1_1.3 mm, optimally 1.2 mm 0 Ministry of Economic Affairs Intellectual Property Office Staff Cooperatives prints diameters relative to CD and DVD, which sets a negligible diameter of 120 mm. However, the optical disc reader can accommodate a disc having a radial diameter of 100 _14 〇 mm, preferably 110-130 mm, more preferably 115-125 mm, and most preferably 120 mm. Even the standard provides a disc with a diameter of 8 cm (80 mm): the base and the clamp ring are the same size as the 120 mm disc, and the depth is the same; only the outer diameter is reduced, reducing the data area of the disc. Commercially available CD and DVD readers and readers accommodate this on individual discs. -23- This paper scale applies to China National Standard (CNS) A4 specifications (210 X.297 mm) 1247894 A7 B7 Ministry of Economics The Property Bureau employee consumption cooperative printed five, invention description (21) diameter disc. Such a disc presents a particular advantage in achieving the present invention, between these advantages being an equivalent reduction in the volume of the assay sample that requires an effect in contact with the entire surface of the disc, and packaging the disc at a sleeve size equal to 3 1/2" The ability of a flexible disk sleeve for such a disc. Even various other standards such as those defined (magneto-optical) "mini discs or 疋-like laser discs have been developed, and other standards will undoubtedly be future development. Therefore, the optical disc of the present invention can have a radial diameter of 50 mm and a large size as a standard laser disc, and can be adapted to a standard size that will be developed in the future. Those skilled in the art will further recognize the term "disc, expecting any suitable rotatable media, which is perfectly circular in any case. As a general rule, having four operational requirements must be consistent with the proper functioning of a typical optical disc system: The reader must accurately monitor and control the focus radial position, tangent position and speed. The control of the radial position and the tangent position is collectively termed as a search. As discussed above and illustrated in Figure 1 B, the disc is transparent. The substrate layer 需要 2 needs to properly focus the reader laser on the reflective layer U4 of the optical disc. Failure to maintain the correct thickness, transparency, and refractive index of the transparent substrate layer n2 may make the reflective layer II4 unreadable. It is discussed that the operational structure encoded in layer 115 is a typical read to maintain proper tracking. In standard pressed Cd, for example, the reader tracks the hole spiral imprinted on transparent substrate i12. In the CD, the reader tracks the spiral track (during the nesting, see below). Figure 4 illustrates the application in a typical cd-category light. Gas of the dish - incident, laser connection -24 - This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 411^&quot;--------------- (Please read the phonetic transcription on the back? Please fill out this page again) 1247894 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed A7 B7 V. Invention description (22) Near-first surface non-operational structure 40; The non-operating structure will be defined by the laser approaching the incident laser's internal focus plane, which is the reflective surface 114. Non-operational structures such as specific analyte signal elements, such configurations will not be detectable by standard devices. The CD-class disc gas-incident, non-operating structure 40 with the laser approaching the first surface is not only the laser approach but also the external focus plane being interrogated by the reader. These non-operational structures will have to be detectable. In the plane of the beam size on the incident surface (800 μm), and finally, if large, these structures 40 may interfere with the reading in the reflective surface 114 due to the intervening characteristics between the laser and the reflective layer 114. Operational knot For this latter reason, a substantial effort was made during the manufacture of the optical disc to determine that the laser of the optical disc is substantially free of defects near the first surface, in part by keeping the surface on the stamp and the main sheet clean and without Dust. Therefore, in order to adapt the standard optical disc technology to the purpose of non-operational structures containing specific analyte signal elements, there is a need for new optical discs and optical disc manufacturing techniques to overcome these problems. There is a need for both disc geometry and tracking design to simultaneously And identifiably extending the signal generated by the non-operational structure disposed on the surface of the optical disc. The single data layer optical disc is a first series of specific embodiments of the optical disc established by the principle of the present invention, thereby concentrating the terminology into a "single data layer" specific Embodiments address these issues by presenting new ways. First, I realized that if the non-operating structure can be placed substantially in the operation - 25 - This paper scale applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm) ------- ------ (Please read the notes on the back and fill out this page) 1247894

When the Ministry of Economic Affairs' Intellectual Property Office employee consumption cooperative prints the focus plane of the structure, the problem of achieving simultaneous focusing on the operational structure and the non-operational structure will be solved, that is, it is substantially confocal with the searchable structure. It will be appreciated that the non-operational structure and the operational structure need not be in equal focus planes - sufficient for the non-operational structure and the operational structure to be sufficiently confocal to allow the optical inter-disc optical read head to detect the two structures. Since the radius of the beam on the operating confocal plane is substantially smaller than the radius of the beam on the first surface of the disc, the result of the refraction of the transparent substrate 112, the use of non-operating aspects of sub-micron is permitted. However, the challenge is that the non-operating structure that will be detectable must be a laser close to the reflective layer 114: unless the reflective layer 114 is clearly transmissive (ie, only partially reflective, see below), placed on the reflective layer 114 laser The remote non-operational structure is not detectable than the non-operational structure applied to the standard CD protection layer 116. In the case of existing optical discs and fabrication processes, the non-operational structure will have to be placed between the transparent substrate 112 and the reflective layer 114 to place them in proximity to the reflective layer U4 and substantially confocal with the reflective layer 114. Such an answer, the separate application of the forced reflection layer 114 after the non-operational structure configuration: the implementation of the optical disc for some fast specific analytes is not maintained. In the new answer to this question, 'because of the standard single data layer cd_ category disc heart flipping the physical orientation (in other words, up and down in the reader), the other laser's far surface will be presented as a light disc. Shoot close to the surface. As shown in Figures 5A and 5B, the conventional CD_class disc is flipped over by exposing the protective layer 116 as a disc with the laser approaching the first surface. If the protective layer U6 is removed, the non-operating structure 136 can then be placed substantially confocal with the L-ray layer 114 and the laser is close. -26- This paper size applies to China National Standard (CNS) A4 specification (210 X 297 mm) A9-------^---------^ (Please read the notes on the back first) Fill in this page) 1247894 A7 V. INSTRUCTIONS (24) The other-solid flip of the disc _ geometrically, relative to the radial flatness of the term, the tangent of the painting is approximately 18 〇. Turn (such as those that have an effect. ... make the double too much change (please read the back note first and then fill out this page). The first type is the physical inversion: properly placed in the standard disc to read the disc reader to read the thunder Shooting close to the structure (that is, the far end of the planar light (that is, moving to the plane "B"), and the standard optical disc is suitably: the structure of the laser distal end for the optical disc-machine reading ((4) plane is turned into The laser is close (ie, moved to the plane "A") (shown in Figure 5a-5b). The first type is "logic, or "image,": from the perspective of the optical disc (target component) of the disc, The geometry created by the operating structure of the disc is now mapped to the image - that is, the reflection is reflected across the curved plane in the radial plane (as defined by this definition) (indicated in Figure 5C_SD). Less difficulty: as I go further, I have found that the "striking" without dyes like the CD-R standard can be very

Easily track, regardless of the swing "grain, for laser proximity or laser far Z embossing. Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative prints, however, when operating structures (especially tracking) established geometric forms The additional "image inversion makes the information unreadable in conventional optical disc readers when the mapped images (i.e., the palms) are non-overlapping. The latter problem can be exemplified by the spiral that is traditionally used for tracking in the CDD format. Therefore, the conventional optical disc reader rotates the optical disc in the counterclockwise direction, as viewed from the viewpoint of the optical pickup of the optical disc, and spirals from the inner diameter of the optical disc ("i.d.") to the outer diameter of the optical disc ("o.d."). This spiral thus appears from the point of view of the optical pickup, as illustrated in Figure 5C. When the disc is flipped and viewed from the reverse side, the spiral is effectively reflected -27. The paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 public). A7 1247894 5. Inventive Note (25) Image in radial plane On the tangent line, the spiral is visualized as shown in Figure 5C, and cannot be *id to 〇(1) in a conventional optical disc drive. For the purpose of further discussion, the phrase "forward video," The conventional orientation in the radial plane as seen by the optical pickup of the optical disc drive for the geometric form established by the optical disc operating structure. In particular, the phrase is thus used to reference the optical form established by the disc trackable structure by the optical disc drive. The optical pickup looks at the conventional orientation in the radial plane. When applied to a disc, the phrase is used to refer to the geometric form established by the disc susceptibility structure itself or the reference image as a projection image. The phrase "reverse image, reference is anti-azimuth", that is, the reference is the orientation obtained by reflecting the tangent of the forward image in the radial plane. Similarly, the phrase "positive The embossing will thus refer to the traditional physical orientation of the operational structure in the direction of the optical axis (between the information layer planes "A," and "B") from the point of view of the optical pickup of the optical disc drive. In particular, the embossment The term "positive relief" refers to the traditional physical orientation of the operational structure of the tracking during the reading of the optical disc, viewed from the point of view of the optical pickup of the optical disc. When applied to the optical disc, the phrase reference is the structure itself. The engraving will refer to the opposite physical orientation. When used as such, the phrase "standard main film, reference to image and relief is typically used to produce a standard disc of a standard disc in conventional processing." The reverse main film is considered to have an image, but is not embossed, as opposed to the conventional one. The present invention is a new discovery that can be easily confocal configured with the optical disk operating structure based in part on the non-operating structure, and another laser is close to the previous application. Anti-28- Private Paper Scale Applicable to China National Standard (CNS) A4 Specification (210 x 297 public) --------- (Please read the note on the back and fill out this page) Ministry of Economic Affairs Intellectual Property Officer Consumer Cooperatives Printed 1244894 Α7 Β7 V. Invention Description (26) (Please read the note on the back and fill out this page) The shot layer. This is the result of manufacturing a complete substrate of the disc (that is, the surface of the substrate contributes to the disc) The layered surface appears to be the most laser-like surface of the substrate (hereinafter referred to as "inverted disc,"). I have found that such a disc can be manufactured by using a conventional master as a stamper, thus producing an operational structure. The forward image is inverted by the embossed image (Hail 1). The manufacturing process, in which the parent or the sub-piece is used as a stamper, is also explained. In this second processing, the main film has a reverse with the conventional The formed image of the forward image has an operational structure presented in a negative relief. Although the new method described above simultaneously establishes optical problems of the focus on the non-operational structure and the operational structure, they do not need to allow such Simultaneous detection of structures. First, these approaches do not need to address the problems of tracking, focusing, and synchronization (speed control) of non-operational structures that maintain such confocal configurations. The non-operational configuration of the second co-focus configuration with the operational aspect (e.g., the tunnel) may cause the signal to be insufficient to be distinguished by the operational (and data encoding) signals to thereby prevent discrimination. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumer Cooperatives. Now I have found that the electronic signals generated during the reading of the optical disc have greater information content than previously known. That is to say, the electronic signal is designed to provide two specific categories of information, necessary information for the operation of the optical disc, and digitally encoded information on the optical disc - in fact, to query and measure in various ways to expose the previously related optical disc entity. The structure is untapped with a wealth of information. Even, I have invented a way to allow the required operational components of these signals to be separated, removing the rest of the signal without disturbing the successful drive operation. - This paper scale applies to the Chinese National Standard (cns) A4 specification (210 χ 297 PCT) 1247894

V. INSTRUCTIONS (27) Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperative Printing, In one embodiment of this feature of the present invention, the new optical disc is manufactured as a signal required for the operation of the optical disc, particularly for optical disc tracking. The signal is separated during the reading by a portion of the signal used to report the reaction of the non-operating structure on the optical disc, while permitting simultaneous detection and identifiable detection while inverting the non-operating structure of the optical disc. Thus, in a first series of embodiments of this feature of the invention, the optical disc is presented to allow the tracking signal to be separated by an HF (RF, Four Columns Sum) signal, removing the HF signal to report information that is configured on the non-operational structure. The tracking signal is separated from the purpose of the HF signal by using a seek design, which is intended to be ignored during the reading of the optical disc by the existing optical disc standard. The wobble "pattern" is formulated in the CD and DVD nestable and refillable standards. The wobble "track" is first added to the disc standard to allow the user to direct the recording (writing) to the CD (and later DVD) media. As stated in the relevant standard, the colloquial term is "orange peel" The sway is detected by the recording device separately during the user's nesting of the data to the optical disc; thereafter, the tracking is accomplished by detecting the data along the spiral writing. This standard and all existing productions of the standard therefore anticipate and guide the wobble to become redundant and therefore ignored after writing. While intended to be ignored during the acquisition by existing standards, the wobble track presents some significant advantages for the tracking design on the inverted optical disc of the present invention. In contrast to a spiral hole, this wobble provides a relatively low frequency signal in H F . Because of the non-operational structure (e.g., specific analyte signal elements), the structure is exemplified by the structure as a 0.5-10 micron size, and the wobble is used as a tracking structure to allow non-operational separation based on the frequency of the tracking signal. One of the higher frequency HF events that occur in non-operating structures is that it can be easily -30- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) -------^--- ------ (Please read the notes on the back and fill out this page) A7

V. INSTRUCTIONS (28) 1247894 (Please read the notes on the back and then fill out this page) Detection and Characterization. By way of example, Figure i3 shows the digital oscilloscope showing the specific immunity attached to the forward image of the present invention, The laser of the embossed inverted disc is close to the red blood cell on the first surface during the searchable scan

(four-column sum, RF) signal disturbance, where the x-axis shows time and the γ-axis shows four columns of summed signal size D. This frequency-based separation of the second, the opposite result is the higher-frequency signal of the non-operational signal For a short duration to prevent the omission of tracking locks. Even a low pass or groove filter can be used to remove the high frequency event by the detector with the reported signal, further determining the correct tracking. It will be appreciated that the filter is omitted from the accompanying signal portion used to detect non-operational signals, such as specific analyte signals. Another major advantage of the wobble track in the disc design of the present invention is that the wobble signal can be used by the drive to maintain a constant linear scan speed at all points on the disc. Given the known wobble frequency, information about the size of the high frequency non-operating events can thus be easily calculated, as shown in Example 3 and discussed further below. Conversely, the disc can be mastered with a track having at least one selected wobble frequency, allowing the linear speed of the disc to be specified for any given point of the radial plane. Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperative Printed In addition, another signal known as a bi-phase indicator signal is the top of the wobble signal encoded on the CD-R disc. The bi-phase indicator signal provides address information that can be used to determine the location on the disc. This address information facilitates the stereo-addressable characteristics of a particular analyte; the space-addressable utility is further applied to the co-owner and the application pending at 08/888, 935, July 7, 1997. No.; application dated July 21, 1998, No. 09/120,049 -31 - This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1247894 A7 __._B7_ V. Invention description (29 (Please read the note on the back and then fill out this page) Note, this document is incorporated herein by reference. In the DVD-R format, similar information is provided using the "patterned pre-fabricated holes", so the pre-formed stamping grooves in the textured area (between the wobbled tracks) encode the address information. In the C D or DVD format used in the present invention, the bi-phase labeling or tread pre-cavity encoding can be selectively omitted from the verification area. Although the reference to non-operational signals is separated by operational signals, the present invention contemplates optical discs capable of digitally encoded information in accordance with CD and/or DVD standards; the manner in which it is described allows for the reading of the digitally encoded information in a non-operational configuration. Reply. And although specifically described with reference to frequency separation within the HF signal, the present invention contemplates that the separation and combination of other types of signals produced by the detector that differ from the four column sum (eg, the seek error signal) can be used to detect and characterize non-operations. structure. Printed by the Consumer Intellectual Property Office of the Ministry of Economic Affairs. Currently, only the CD writer (reader) contains the ability to detect the swing. Since the wobble is typically detected only during writing, most of the fabrications of the present invention are not required and the laser may be processed to interfere with the amplitude of the non-operating structure detection, specifically designed for quality control purposes. The device is utilized in Example 2 and Example 3. This device is capable of detecting and tracking the swing without forcing the laser to pulse the desired energy for the disc to write. However, it will be appreciated that a driver capable of detecting the tracking structure, whether or not it can be written, can be utilized in the implementation of the present invention. Examples 2 and 3 thus presented illustrate the successful use of the single data layer and the forward image embossed inverted optical disc of the present invention ("detecting IgG in human blood by immunoassay, and (2) using minimal Corrected CD reading _ -32- 冢 Paper scale applicable to China National Standard (CNS) A4 specification (210 X 297 public 3 &quot; 1247894

, invention description (3〇) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed "machine speculation and characterization of human red blood cells captured on the surface of the disc. Example 7 actually illustrates the adaptation of the nucleic acid base assay to the detection principle thus described. Disc Manufacturing The first process (Process 1) for fabricating a single data layer, forward video disc of the present invention will now be described with reference to Figures 3A-3D. Process 1 forms a disc having a positive relief operating structure. The transparent disc substrate 112 can be borrowed. It is manufactured by an injection casting process in which the polycarbonate solution is injected into a casting cavity having a "die." The stamper is made of a disc master by means of an electric cymbal, which will be more fully explained below. The Jiangcheng casting process typically takes 5 to 10 seconds per disc. Figure 3A illustrates three steps in a standard electroforming and substrate processing, followed by a final injection casting (stamping) step, a processing (stamping) step for creating a complete substrate 112 of a single data layer disc having a wobble track, cd-R or CD_RW disc (for further details, reference is made by Williams, &quot;The CD-ROM and Optical recording Systems^, Oxford Press, 1994 'hereby incorporated herein by reference). The main piece 510, the parent piece 520, the mother piece 530, the sub-piece 540, and the optical disk substrate 112 are displayed in a side cross-sectional partial view. Briefly, the process can begin with the creation of the master disc 510 (made of glass, plastic, etc.), which is performed prior to the steps shown in Figure 3A. In step 505, the master disc 51 is placed in the current nickel electrolyte bath, where the disc acts as the cathode of the electronic circuit. The nickel anode is used to deposit a layer of nickel on the surface of the main optical disc to establish a nickel "parent sheet, 52 (^ then 'the nickel parent sheet 520 is separated by the main sheet 510, typically (but not always) at -33- This paper size is suitable for towel 0 @家鲜(CNS)A4 specification (210 X 297 public) -^ -------Book-line (please read the note on the back and fill in this page again) 1247894 A7 B7 V. Invention Description (3) The main film 510 is destroyed. As a result, the parent film 52 is embossed with the negative relief image of the main film 51. (Please read the back note first and then fill in the page) The parent sheet 520 can be used directly as a stamper in the injection casting process 535 but has been shown to be more efficient in the related art to produce a majority of equal copies of the parent sheet 520, termed "sub-slices, 540. From the original nickel portion The established family of electroformed parts is termed "base,". Thus, the parent 520 is typically used galvanically in step 515 to produce a plurality of equal "masters", 530. The master 530 has a relief Presenting the previous image. The majority of the equal master 530, in turn, is used in step 525 The majority of the sub-slices 540' image orientation and embossing are equal to the "parent film, 520, and thus have a reverse image of the negative embossing presentation compared to the main film 510. In step 535, the sub-slice 540 is used as the optical disk substrate U2. Injection molded stamper. The disc thus manufactured has a forward embossed image in front of the embossing. Fig. 3C shows a CD-R disc 11 having a substrate 112 as manufactured in Fig. 3A having a wobble track 118, a reflective layer 114 and a protective layer 116. The optical path and focus on the wobble track are shown in dashed lines. As can be seen, the substrate 112 of a typical optical disk is presented with its laser-like surface that is attached to its patterned surface. Employee Consumption Cooperative Printing Figure 3B shows a process as described in the present invention (hereinafter referred to as "Process 1") for establishing a single data layer of an image prior to the present invention having a embossed operational structure. Unlike the standard process shown in Figure 3A, in this process, the master 530 is used directly in step 625 to stamp the disc substrate 13 2. As illustrated in Examples 1 and 5, this is reported here. The disc used in the experiment was produced in EXIMPO SRO (Prague, Czech Republic) using this modified matrixing procedure. -34- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperative, Printed 1244894 A7 ______B7___ V. Invention Description (32) One of the advantages of Process 1 is the standard main film - such as CD-R main film or CD-RW main film - or other easy to use existing The master film of technology and device construction can be used to fabricate the forward image inverted optical disc of the present invention. In Process 1&apos; as illustrated in Figure 3B and Example 1 and Example 5, the master piece is discharged in a standard injection casting machine to some degree of difficulty in the use of the injection casting process. The various parameters of the injection of the pound machine will be apparent to those skilled in the art and will be adjusted so that the molten polycarbonate flows properly over the mold. For example, the temperature at which the polycarbonate is injected is raised to determine that the molten polycarbonate is less viscous. While this increased temperature may alter the optical properties of the polycarbonate (e.g., birefringence), the optical properties of the polycarbene layer 132 are intangible to the single data layer inversion of the present invention because the laser never passes through the data layer. Figure 41 - 411 presents a mold set for use in the manufacture of a disc manufactured as set forth in Example 5. Figure 3D shows a disc formed by process i, while non-operational structure 136 is in confocal application with reflective layer 134 and the laser is close to reflective layer 134. As can be appreciated, the substrate 132 in the single data layer inverted optical disc of the present invention is presented with its patterned surface laser near its planar surface. As can also be appreciated, the disc thus produced has a forward embossed forward image. It will of course be understood that the inverted surface of the optical disc is a "planar surface" that does not require a very flat surface because it is not located in the optical path. Therefore, the surface is thus cleaned into an "extra, surface" of the optical disc substrate. In a standard optical disc and the forward video/positive embossed inverted optical disc of the present invention, the structure of the tracking is _ the oscillating "track" is in the information layer plane "A,", that is, respectively in Figure 3C and The information layer laser shown in 3D is close to the plane—------ order--------- line (please read the notes on the back and fill in this page)

A7 1247894 V. INSTRUCTION DESCRIPTION (33) 0 However, in a standard optical disc, the plane "A" is the plane of the information layer closest to the plane surface of the substrate. In contrast, in the forward video/positive embossed optical disc of the present invention, the plane "A" is the farthest plane of the information layer of the plane surface of the substrate. The result of the structural orientation of the latter, and despite the colloquial use of the term "striking" of the tracking structure, I have found that the non-operational structure 136 is disposed on the reflective layer of the single-data layer forward image/positive embossed disc. Tendency = set themselves in the center of the adjacent tracking structure itself rather than falling within the tracking structure itself, as shown in Figure 3D. The second process for fabricating the single data layer of the present invention, the forward image inversion disc (hereinafter referred to as For "Process 2," will now be explained. The disc manufactured using Process 2 has an operational structure to present the forward image with a negative relief. In Process 2, the master disc is created as an image having the desired operational structure as opposed to the conventional one. Basically, as shown in FIG. 3A, immediately after the conventional electroforming and the substrate processing, when the surface of the surface is read by the surface laser (ie, inverted), the (four) substrate has an operation button. Negative relief before the image. Thus, in contrast to a standard optical disc (shown in Figure 3C) and the optical disc of the present invention (shown in Figure 3D) as manufactured by Process 1, the optical disc of the present invention (shown in Figure 3E) as Process 2 has a plane "B" The tracking structure, that is, in the far-end plane of the information layer laser. The plane "B" in these inverted discs is the plane of the farthest information layer on the plane surface of the disc substrate. As a result, non-operational structures placed on the reflective surface will often be centered on the tracking aspect. This situation should desirably increase the signal to the Chinese National Standard (CNS) A4 specification (210 X 297 public:----------) at the same non-36-paper scale set at the center of the adjacent track. ---------Book---------Line (please read the phonetic on the back? Please fill out this page again) 1247894 A7 V. Invention description (34) Operation structure can get signal With the forward image/positive embossed inverted disc (Process 1) or the forward image/negative embossed inverted disc (Process 2) of the present invention, the reflective layer 134 is now presented as the first surface of the disc should be apparent, without The transparent substrate layer U2 is presented to assist in laser focusing, such as a standard optical disc. Figure 6A previously explained the problem to an image/positive embossed inverted disc. One simple solution shown in Figure 6B is to add an additional focusing lens 17 to the optical pickup of the optical disc. In order to produce the data presented in Examples 2 and 3, the reader is modified as such. Alternatively, or additionally, the distance between the optical pickup and the first surface of the disc can be adjusted so that the laser can be correct. Focus on the first surface of the disc. Alternatively, a preferred embodiment, as further described below, adjusts the optical disc itself rather than the reader. In the preferred embodiment, the non-unitary or integral laser refractive member is attached to the disc laser with a cover plate. Approaching the side to create a disc assembly. This cover acts as a refraction, and thus further focuses the incident light on the disc operating plane. Traditionally, this plane of operation is now calculated as the second surface of the disc. Although currently preferred, it can be installed The hinged or otherwise adjustable integral cover can also be used. In Process 1 or Process 2, the standard CD_R mold _ is stamped in standard i 530 in process i or reverse image parent in process 2 52〇 or sub-piece 54〇-produces a disc substrate with a thickness of L2mm. The addition of the cover increases the thickness of the disc assembly beyond the thickness of the standard ii2mm which is specified in the standard. The 24-deep disc can still be read, but it is still expected to add the cover circle to the substrate 132. Environmental thickness 37- This paper scale stabbing the standard (CNS) A4 specification (210 X 297 公爱— ^ -------tT---------^ Αν. (Please read the notes on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1244894 A7 ___ B7 V. Inventive Note (35) Reduction. In the new approach, the depth of the substrate 132 can be reduced by using the DVD-R mold in the final step of Process 1 or Process 2. As will be appreciated, the c D - R format The stamp (process 1 is master 530, process 2 is reverse image master 520 or sub-sheet 540) must be fabricated to fit in the mold, and the mold settings must be adjusted to determine proper stamping. The substrate thus formed is 0.6 mm; and with a cover plate of 1·2 mm thickness, the thickness of the combined optical disc assembly is therefore 1.8 mm. Regardless of the solution or combination of solutions for adjusting the focus, the optical limitations of the selected composition of the single data layer inverted optical disc geometry eliminating substrate 132 of the present invention are significant relative to the optical limitations of the transparent substrate 112 described above. That is, since the present invention alone layer 132 of the inverted layer of the data layer is not used to refract incident laser light, as opposed to the standard optical disk substrate 112, the layer 132 is transparent, the refractive index and thickness can be adjusted independently of these optical parameters. This situation presents manufacturing advantages that are not easily achieved with standard optical discs. However, it is preferred to fabricate a single data layer optical disc of the present invention using polycarbonate as a mounting base for an existing optical disc manufacturing apparatus, as explained below. To illustrate these principles, it is possible to construct a track-receivable optical disc having a simultaneously readable non-operating structure, and a single data layer forward image/positive embossed inverted optical disc is manufactured as detailed in Example 1. Each of the optical disk substrates 132 is composed of injection-cast polycarbonate, and includes a wobble track forward image having a pitch of L6 μm and a wobble frequency approximately as stated in the C D - R standard. The gold layer is disposed near the surface of the laser to form a reflective layer 134. In the first series of experiments, detailed in Example 2, the specific verification point specific to the human immunoglobulin G (IgG) was constructed on a small portion of the gas incident gold-38- _ paper scale applicable to the Chinese national standard (CNS) A4 specification (210 X 297 public 1) ----------------------------------------------------------------------------------------------- On the surface (please read the note on the back and then fill out this page) As shown in Figure 7A, the test grid is constructed as a three-layer sandwich; it will be understood by those familiar with the relevant art of clinical validation, the purpose of the sandwich To present the final sandwich layer, antibody 76 is used for analyte capture and detection. It will be appreciated by those skilled in the art that the assay itself contains many such three molecule sandwiches, only one of which is In the first series of experiments, antibody 76 was selected for human immunoglobulin G (IgG) because of its specificity, and was found in normal human blood at a concentration of approximately i 1 g/dl. The human blood sample taken by the person is applied to the light The grid is checked and simply incubated. The disc is cleaned and then "developed" by the application of a 3 μιη latex sphere, each sphere previously coated with a human IgG specific antibody. The disc is again Cleaning. As shown in Fig. 7B, the result of this treatment is that the IgG-mediated specific adhesion of the latex sphere 79 to the disc. When the blood sample lacks IgG, the latex sphere will be removed during the cleaning. Printed by the Consumer Cooperative, Figure 8 shows a video image captured by a MG-specific optical disc in a microscopic view of the light. The m latex sphere follows the human blood and the antibody-co-rubber latex sphere. The latex sphere is prone to occur, such as the wobble track itself. In the video capture image, the darker area is the positive tracking structure. The magnification is precluded from observing ''grain, continuity.') The video capture image suggests that not all but also many latex spheres are located adjacent to the anterior/positive embossed disc. The upper wobble track. It must be easy to appear as a part of the signal relative to the width of the groove. It is advantageously adjusted to enhance the positioning of the non-operating structure. Example 4 -39- - ί Μ / y Μ &lt; υ 1 &lt;1 V η / U τ 1 V Dry 1247794 A7 B7 V. Description of invention (37) The calculation of the optimum relationship between the size of the signal and the width of the track. In the environment, the forward image/negative embossed inverted disc is used. Similarly, the size of the molecular range on the checkpoint is determined. The three-molecule vitamin H_streptavidin_vitamin sandwich can advantageously be adjusted to enhance the robustness of this signalling, but limit the movement to allow for this positioning. U.S. Patent Application Serial No. 09/120,049, filed on Jul. 21, 1998, and Serial No. 08/888,935, which is incorporated herein by reference. This is described in the application of July 7, 1997; the disclosure of which is incorporated herein by reference. Even though, the shape of the track itself can be adjusted in the CD and DVD specifications to enhance the positioning. Figures 9 and 1 show the atomic force (atm) microscopic image of a single latex sphere attached to a disc, which is slightly higher than the magnification used in Figure 8. It is easily apparent in Fig. 9 that the pendulum itself and the latex sphere are positioned at the center of the turn. Figure 10 presents quantitative data for AFM-take. The groove was measured at 171.70 nm deep. The height of the latex sphere beyond the bottom of the groove is 2.407 μιη. Figure 11 is an atomic force microscopic image of a di latex sphere in a single microscopic view. This quantitative section analysis explains the consistency of the latex spheres used to develop this antibody-IgG immunoassay. In addition, the quantized segment analysis identified that the horizontal distance between the center of the grain and the center of the adjacent grain was 843.75 nm (0.84 μιη)' and the desired 16 μηι track was excellently matched. The second series of experiments reported in Example 3 demonstrates that such a non-operational structure can be reliably detected by the minimally modified CD-R device as a high frequency, high amplitude variation of the HF signal. That is to say, the above single material with the wobble track -40 - This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) (please read the note on the back and fill in this page) --- -----Order---------Line· Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing A7 1247894 B7__ V. Invention description (38) Layer, forward image / positive relief first surface verification The disc allows the tracking signal and the signal generated by the non-operating structure configured on the surface of the disc to be simultaneously captured and recorded (please read the phonetic note on the back side and then fill out this page). As further illustrated in Example 3, the optical disc was prepared as set forth in Example 1. The assay grid was prepared essentially as in Example 2, but the anti-gilyC〇ph〇rin antibody was substituted for the anti-IgG antibody 76 used in Example 2. Glycophorin is a protein that appears on the surface of all human red blood cells (red blood cells, RBC). A liver lipidated sample of human blood (1 μ μ1) is applied to the assay grid, and the disc is simply washed after the disc. Figure 1 2 shows the atomic force microscopic image of the RBC immune specific attachment to the disc verification grid. As indicated by the quantitative analysis, the RBC level was determined to be 7.984 μιη ' and the red blood cells (8 μπι) were known to have an anomalous diameter; this size and Example 2 were significantly different from the uniform 3 μπι diameter of the observed latex sphere. The height of the RBC beyond the bottom of the groove is observed as i 8 μπι. As further illustrated in Example 3, the CD-r device for use in the quality control of the optical disc industry is manufactured by cd Associates, Inc. (printed by the Intellectual Property Office of the Intellectual Property Office of the Irvine Department of Economics, California) for reading the optical disc. The CD-R wobble tracking system (mode RSLi 〇 0) of the driving device is added to the lens 17 to correct the optical pickup to adjust the focus on the optical disk lacking the refractive layer 112, and the height of the rotating shaft is also increased. The HF signal (i.e., RF or quad sum) is amplified by the electronic circuitry in the RSL 100 so that the oscilloscope can be provided without adversely affecting the performance of the wobble seeker. Figure 13 shows a representative track, showing the time on the x-axis and the four-column sum signal size on the γ-axis. ' Figure 1 3 shows that red blood cells are directly visible as c D _ R Reader η f -41 - This paper scale applies China National Standard (CNS) A4 specification (210 297 297 public) 1247894 A7 B7 Ministry of Economics intellectual property Bureau member. Workers' consumption cooperatives printed five, invention description (39 nicknamed South frequency, high amplitude events; analysis of mammalian cell size and T 'no latex sphere or other external factors to send a part of the required The specific analyte signal is generated. It is also apparent from the oscilloscope of Figure 13 that the difference in the hf baseline is a double peak. Although red blood cells are known to have a characteristic biconcave shape, we have observed this double peak in Example 2.値 is used to report the presentation of analytes. When a laser traverses a sphere, this double peak appears as a regenerative change in reflectance. Such regenerative electronic markers can advantageously be used for non-operating structures of various sizes. Identifying and discriminating signals. From the oscilloscope trace of Figure 13 to another observation, it is easy to see that the baseline on each side of the signalling event is stable; that is, The tracking of the moving track itself does not cause major changes in the four-column sum signal. For example, the optical reader of the CD-R standard maintains a constant linear velocity regardless of reading at any position on the optical disc, and corrects the rotational speed of the shaft to lock the wobble pattern. The frequency of the channel. According to the known linear speed of the optical disc and the increased indication on the oscilloscope tracking, the division on the oscilloscope tracking can be displayed as equivalent to the linear distance of the optical disc of 1 3 μιη. As shown in Figure 3, the oscilloscope tracking measurement The difference in the four-column sum signal reference line is therefore set to 1 〇μπι, which approximates the non-corrected size of the object in the direction of the tracking track. The actual size of the object is small. Calibration and non-wave using a 3 μιη latex sphere reader The oscilloscope has been given a peak size of 5 μιη, which is 2 μπι wider than the actual object. This situation is appropriate to consider the laser focus diameter of 1·5 μηι on the first surface of the verification disc. The actual diameter of the laser diameter on the surface of the disc ------—^--------- (Please read the notes on the back and fill out this page) 42- 1247894 Α7 Β7 When the 2 μιη difference between the invention and the invention description (40) is printed by the Intellectual Property Office of the Ministry of Economic Affairs, the event captured by the oscilloscope in Figure 13 is the high-frequency and high-amplitude difference of the four columns of the sum signal, and the size of the object is reported. It is known to coincide with the known 8 μιη human red blood cells. Figure 14 shows another oscilloscope trace of the HF event detected by the isolated red blood cells on the same disc. The two-phase peak is more significant. Figure 15-17 is an additional example. Figure 18 is a digital overlap of most events amplified from different regions on the same disc, actually illustrating the regeneration of size and shape measurements on different red blood cells of a particular immune attachment to the disc. In summary, examples 1-3 actually illustrate a micron-sized non-operating structure configured on a first surface of a single-layer inverted optical disc constructed as described herein, which can be detected and measured by a minimally modified standard optical disc reader. And characterization. The optical disc operating structure including the tracking structure is a simultaneous use of a single optical pickup for simultaneous detection with non-operating structures and for easy identification by non-operating structures. Example 2 specifically illustrates that immunoassays for small molecule analytes can be readily adapted to detect using this system; Example 3 specifically illustrates that cell counting and cell analysis are also readily accomplished. Example 7 in turn illustrates that the assay based on nucleic acid hybridization can be similarly adapted for detection using the track-receivable optical disc of the present invention. As detailed in Example 7, the solubilization phase hybridization is performed in parallel with various concentrations of the target nucleic acid present in the following constants. (〇 sequence to the first target (3 'probe)) complementary single-stranded nucleic acid probe The needle and (2) sequence to a second sub-target ("5, probe") complementary single-stranded nucleic acid probe. The 3, the probe is further conjugated to the paramagnetic bead and the 5, the probe is further Conjugation to ------- order--------- line (please read the note on the back and fill out this page) -43- A7 1247894 _____B7_____ - V. Description of invention (41) Protein: Paramagnetic beading acts to allow magnetic separation and purification of some duplex targets, and further allows direct detection by a disc reader; 5' probe protein allows a portion of the metal surface to be applied to the metal surface of the first surface of the disc of the present invention. Streptavidin captures part of the duplex target. Figure 36 illustrates the spotting point at the visual time. The direct attachment to the gold surface of the trackable disc is formed by the Wedworth force and between the free sulfyhydryl streptavidin protein and the disc gold surface. The sulphide-gold bond is bonded to the streptavidin coating. The streptavidin captures 5, the probe's vitamin Η-portion. The 5' probe is in turn supplemented by 14-nucleotide Watson-Crick to capture the target nucleus sequence at the target 3' end. The target is sequentially captured at the 5 f end via the Watson-Cirick of 1 4 nucleotides to capture the 3 end, thus the tether Dynabead^ to the disc. Figure 3 7 shows the optical micrographs of the optical discs taken separately, each disc is magnified Rate 2: Attached spheres and oscillating ridges are clearly visible on all 3 discs with higher magnification panels. The increased number of attached beads to increase the amount of nucleic acid target is clearly visible' while Disc 3 (Figure 37C) shows supplements The driven sphere is attached to the surface of the disc with a 20-millionth molar (20X10·21 mol; 12xi〇3 molecule) nucleic acid target, while the disc 2 (Fig. 37B) shows the complementary driven sphere at 20 billion One mole (20xl0-i8 mole; 12 χι〇6 molecules) nucleic acid target attached to the surface of the disc, and the disc i (Fig. 37Α) shows the complementary drive sphere at 20 teraflops (20χ10- Ϊ́5mol; ΐ2χι〇9 molecules) nuclear The target is attached to the surface of the disc. No beading is observed on the surface of each control disc (not shown). Appreciation is shown in Figure 37 at each of the shadows&gt; Τ 7. The main part of the verification field. ---Order---------Line (please read the note on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing -44-

Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 1247894 A7 B7 V. INSTRUCTIONS (42) Example 7 Therefore, the actual verification of the nucleic acid hybridization benchmark can be easily adapted to be detected using the optical disc of the present invention. Hybridization to nucleic acid arrays can be readily adapted between assays that are used to detect nucleic acid hybridization using the optical discs of the present invention, such as U.S. Patent Nos. 5,143,854; 5,405,783; 5,445,934; 5,510,270 No. 5,800,922; 5,556,752; 5,599,695; 5,744,305; 5,770,722; 5,837,832; 5,843,655; International Patent Publication No. WO 98/12559 (Dmers), and in Nature Genetics 21 (Suppl): Further reference is made to 1-60 (1999), which is incorporated herein by reference. Example 7 further illustrates the practical use of magnetic beading, which has long been used in the separation and purification of molecular biology. It will now be used directly to signal, providing an effective system for separation, purification and detection without forcing physical radionuclides, fluorophores, leavens. A chemical moiety or analog is additionally labeled with a nucleic acid. Co-owned and co-pending application Serial No. 08/888,935, filed on Jul. 7, 1997; No. 09/120,049, filed on July 21, 1998, hereby incorporated herein by reference, Illustrating various other signal elements and chemistries will allow for the adaptation of existing assays to the use of the presently searchable optical discs. In the various uses previously mentioned, the optical disc of the present invention allows for independent, stereoscopically decomposable events. A single data layer disc change will be known to those skilled in the art, a single data layer specific -45- paper scale applies to the Chinese National Standard (CNS) A4 specification (210 x 297 mm) •------- ------------Book ---------^ (Please read the notes on the back and fill out this page) Ϊ247894

V. INSTRUCTIONS (43) Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumers' Cooperatives. Many changes in the CD-ROMs can be made. Most of the specific embodiments will be discussed below. Full Image Operation Structure Referring now to Figure 2, a single data layer embodiment is shown. In this particular embodiment, the operational structure of the optical disc is encoded in a reflective holographic image rather than being physically pressed into the optical disc substrate. The reflection hologram allows the physical plane of the operational structure to be removed by the physical plane occupied by the non-operational structure. Most data layer (information layer) discs, as further explained below, present another way of separating the operational and non-operational structures. The optical disk 190 includes a disk substrate 192, a full image 194, and a transparent protective plating 198. The hologram 194 is a reflection hologram containing the operational structure required for the optical disc 190. In particular, when the laser is reflected by hologram 194, it will occur if the oscillating track of the correct orientation appears on omni-image plane 195. In a preferred embodiment, the holographic image plane 195 is laser-proximate to the holographic solid plane 194 and substantially confocal to the non-operational structure 196 disposed on the first surface of the optical disc. The laser, as previously focused on a plane shared by the non-operating structure and the operational structure (here, the wobble track image), allows manipulation of data (specifically tracking data) and specific analyte data. Of course, it should be apparent that the laser is optimized to focus on the holographic image plane - confocal with the non-operating structure - requiring the laser to focus less rigorously on the holographic solid plane. The hologram is very natural to not only tolerate the "error, but also to benefit from it. As is known in the art of optics, the full-image solid surface of each part can produce image integrity, interferingly encoded on the surface, However, if the size of the irradiated part is reduced, the resolution is reduced. Conversely, the larger part of the whole image is -46- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) '------- Order --------- (Please read the notes on the back and fill out this page) 1247894

Invention Description (44) 2 'Image is preferred. Therefore, the illumination laser spot is large, and the disc operation image is preferably - in the preferred embodiment, the _ wobble track. As will be appreciated by those skilled in the relevant art, the holographic image plane can also be advantageously projected, so that it is no longer correctly confocal with the non-operating structure, as for non-operational structures such as wobbled tracks that are simultaneously with a particular analyte signal. Can detect,. Therefore, the image can not only be close to the plane of the full image, but also the far end of the laser. Even though, π is integrated into the disc 190, the full image 194 is removable. This situation allows the hologram 194 to be mass produced using existing high speed holographic printing processes. Even, depending on the application, the hologram 194 can be attached to the disc substrate 192 in the opposite direction, potentially allowing the substrate 192 to be reused. The present invention can be used to determine the holographic image of a disc. A single data layer embodiment allows a wide range of optical discs that can be read by a disc reader to be mass produced at low cost. In another embodiment of the present invention, the optical substrate 192 does not need to conform to the optical requirements of the standard transparent optical disk substrate 112 because the optical disk substrate 192 is located at the laser distal end of the data plane. Also in the specific embodiment described above, wherein the operational structure is embossed and reflective coated, respectively, the holographic embodiment may often advantageously include a laser-assisted, non-integrated cover that assists in focusing. In the holographic embodiment, the focus can therefore be adjusted on the holographic image plane.

The regionalized CLV is a single data layer disc that is physically imprinted and holographically encoded as described above, and both can often utilize a different tracking design than the CD_R standard wobble track. In particular, the optical disc of the present invention can be applied to the Chinese National Standard (CNS) A4 specification (210 x 297, mm) at a size of -47-book paper outside the optical disc area according to the present invention (please read the precautions on the back side and then fill in the form) Page) t Order---------Line· Ministry of Economic Affairs Intellectual Property Bureau Employees Consumption Cooperative Printed 1244894 A7 V. Invention Description (45) (ZCLV) format principle "Zoned Constant Linear Velocity" force to establish. As explained briefly herein, the (ZCLV) format is well known in the art and is detailed in various industry standards, including the DVD_RAM specification (see, for example, ECMA 272, incorporated herein by reference). As shown in side view 21 of the above, the ZCLV disc 200 has a data area 202 which is divided into a plurality of areas 204a-204e. Although only five areas are displayed, the actual ZCLV format disc can have a different number of areas. For example, the dVD-RAM ZCLV format has 24 areas in the nestable data area. Each of the regions 204a-204e is divided into a plurality of sectors 206. The inner region has fewer sectors than the outer 4 regions because the inner region radius is smaller than the outer region radius. The layout of the disc is configured such that the block header information on each of the magnets (i.e., the spiral turns) is radially aligned within each sector. This situation allows the embossed, non-oscillating head region for each data block to be followed by the "swing pattern and track" where the data can be written. In use, the optical disc reader can rotate the ZCLV disc at a constant rate in each region and still maintain a constant data rate in the region. For the internal area, the disc must be rotated rapidly to maintain a substantially overall constant rate, while for the external area, the disc can be rotated at a lower rate. Figure 22 shows a partially enlarged perspective view of one of the sectors of the ZCLV disc 2, as can be seen, most of the tracks 220 are radially arranged within the sector, so = magnetic hold has "pre-groove, area The head of the 222 embossed embossed data block can only be recorded after the oscillating groove and the oscillating ridge area between the oscillating land and the ridges in the wobbled area 224. For use as a verification optical disc as in the present invention, the non-operational structure 236 can be configured at -48-X 297 mm. Sheet size scale ((3)S) A4 size (6) A7 1247894 V. Invention description (46) (Please read the back Precautions Fill out this page again) The wobbled surface and track area 224 of the sector on the disc, as shown in Figure 23. Since the wobble land and the track area 224 form a continuous area in each sector, the verification of the disc using the ZCLV format can perform different tests using the sectors. The embossed embossed head information in the pre-formed crepe region 222 can be used to store information identifying the swaying ridges and the characterization of the crepe regions. The oscillating surface and track in the oscillating surface and the track area and the floating embossed data track in the pre-formed track area satisfy the functional requirements of the optical disk reader. As suggested above, the features of the various digitally diverse optical disc (DVD) standards can advantageously be utilized in the implementation of the single data layer embodiment of the present invention. Therefore, the present invention is not limited to the current *CD_type disc or *CD standard. In addition to the ZCLV format specifically utilizing most of the assay discs discussed above, it has become apparent that smaller structure sizes and reduced wavelength lasers developed in the DVD standard allow higher density non-operational structures to be more likely to use the €13 standard. For high stereo identification. Adjusting the DVD_RAM standard is another major advantage of the invention for image-inverted discs. It is the pattern of the groove-pattern (refer to ECMA 272), which can be used for non-operational structure detection and characterization on optical discs. The tangent resolution is doubled. Even the two-tiered material layer DVD format that provides unique features will be discussed further in the letter section. Disc cover

The CD-R readers used in Examples 2 and 3 have been corrected by the addition of a focusing lens to take into account the lack of a specific analyte CD--| Adjust the focus of the drive when the substrate is on. It is often preferred to use the other way to adjust the light itself rather than the optical disc reader. In the later i-type, the 'laser refractive member is attached to the laser laser approaching side and printed for the cover company I_ :49- This paper size applies to the Chinese National Standard (CNS) A4 specification (21G X 297 public) )----------- A7

1247894 V. INSTRUCTION DESCRIPTION (47) A plate; the cover acts to refract and thereby focus the incident light on the refractive surface of the disc. When designed in a cooperative manner, the cover plate is alternated in the focusing optics of the drive unit. The material presented in Fig. 4A is obtained using such a cover plate without the aid of another focusing lens 17. This way is depicted in Figure 19. Here, by conventional means, the laser light is incident from the bottom of the side cross-sectional view. The optical disc 130 includes a disc substrate 132 and a reflective layer 134, and the non-operational structure 136 is disposed on the reflective layer. The oscillating ridges 138 are pressed in the substrate 132 and plated by the reflective layer 134 as indicated. Also shown in the figure is a non-integrated cover plate 140. One embodiment of such a cover is further shown further in Figure 35 in the above perspective. Preferably, the optical disc assembly (disc plus the attachment cover) is sized to approximate the size of the monolithic disc, that is, the depth is i 2 mm and the diameter is 80 mm or 120 mm. However, it is also contemplated that the disc assembly can be varied in size. In these latter cases, regardless of changes in the physical size specifications of the disc standard, the assembly must still demonstrate compliance with the necessary optical and mechanical requirements of the drive: between these requirements, the laser must be properly focused on the disc operating plane, The disc assembly must properly clamp the spindle, and the disc assembly must not change much with the standard weight so that the drive motor cannot maintain the proper rotational speed. Example 6 presented here below illustrates that the cover plate made of polycarbonate is approximately 1.17 mm thick, but otherwise the size is equivalent to a standard 120 mm disc. The cover is made of polycarbonate to take advantage of the well-known optical qualities of polycarbonate and the ready-to-use properties of the device for its conversion. However, as discussed further below, other plastics can be advantageously used to construct the optical disc cover. -50- This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) · _ ------- order --------- (please read the notes on the back first) Fill in this page) Ministry of Economic Affairs Intellectual Property Bureau Employees Consumption Cooperatives Ministry of Printing and Economy Ministry Intellectual Property Bureau Employees Consumption Cooperatives Printed 1247894 A7 r------ _- B7_ V. Invention Description (48) Single material as in Example 5 The layered disc is 1 2 +/- 〇〇 5 mjn thick, and the single data layer disc manufactured as in Example 6 is approximately 117 thick. The physical thickness provided by the Red Book Standard (1.1 - 1·5 mm for all combination layers) is externally combined to a depth of approximately 2.4 mm for the second cover. Although this situation does not present an optical problem - maintaining the optical path within the specification - it appears that increasing the thickness for this may present the difficulty of clamping the optical disc assembly in the drive. Even the new disc and cover exceed the standard, unified disc weight. However, experimentally we have undesirably found that the jaws and rotating components are not problematic. The data presented in Figure 40 is obtained using the optical disc manufactured as in Example 5 and the cover assembly manufactured in accordance with Example 6 prior to reading by the optical disc drive. The cover plate provides sufficient assistance to focus to exclude the addition of another focus lens to the disc player pickup to produce the information set forth in Figure 4A. In a series of separation experiments (not shown), we removed the jaw ring from the disc before attaching the cover, allowing the drive to couple the disc assembly by clamping the 12 min thick cover separately. Also as discussed above, the disc itself can be reduced in thickness by stamping in a DVD-R mold. Although non-integrated components are currently preferred as members of a laser refracting optical disk cover, a hinged or otherwise movably attached integrated component or an integral component that cannot be moved can also be used. The non-integrated member can be attached to the disc in reverse (removable) or non-reverse depending on the desired use. To produce the information presented in Figure 40, a non-integrated polyacid seal cover made as in Example 6 can be reversely secured to a disc as manufactured in Example 5. Two to three small drops of methyl copper (barium), which can partially dissolve the polycarbonate and make it sticky, are applied to the disc clamp ring 142. Commercially available paint-51 - This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) ------- order--------- line (please first Read the precautions on the back and fill out this page.) 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau Employees Consumption Cooperative Printed 5. Inventories (49) Levels are used. The cover is then gently embossed on the disc for approximately 3 sec. The MEK permanently secures the cover to the disc clamp ring; on the outside diameter of the assembly, the disc and cover remain tightly juxtaposed but not attached. The optical and mechanical requirements of the system require a compact radial registration of the disc and cover to prevent the disc from leaving the circumference; abnormal behavior during rotation prevents the servo from locking the tracking signal. To determine proper registration, the optical disc of the present invention and the mating non-integrated cover will preferably have non-operative configurations that are coupled to each other and/or interlocked with one another. In a better way, the cover of the child will overlap with the disc. The release cover made in Example 6 lacks an engineered structure, and is positioned in the center of the disc as follows. In the start-up work, the plastic tray of the CD holder ("jewel box") is used to make a single data layer forward image. The embossed inverted disc cannot be moved. The MEK is applied to the disc clamp ring with the dropper and the cover is placed at the top and pressed to the correct position. The deformable shaft in the bracket keeps the disc and cover tightly registered to allow for success. The combination of these work, the simple exclusive t-set was reformed to make up for the registration. The discs and cover plates manufactured as in Examples 5 and 6, respectively, have stacked loops 144 'will push the 垓 142 clamp ring 142 Separated by the data area. The data area is protruded from the optical disc (and the cover) but not on the other side. When stacked, it is designed to keep the adjacent optical discs at a sufficient distance from each other to prevent scratching. Therefore, the stack is combined. The disc and the cover are facing each other. MEK is used here in the experimental sample because it allows for convenient and rapid attachment of the polycarbonate. Other adhesives can be used and need to be in addition to polycarbon. -------^ - -------- (Please read the note on the back? Please fill out this page again) -52- A7 1247894 _B7 V. Description of the invention (5〇) (Please read the notes on the back and fill out this page) Plastics other than acid fat are used for the cover and/or optical disc. Even MEK frosts the surface of the polycarbonate; although its application to the clamp ring 142 does not present an optical problem, as it is located outside the data area, if it is combined with data MEK cannot be easily used when needed in the zone, and for these purposes, optically more suitable adhesives will be preferred; among such adhesives, those typically used for attachment A separate layer of adhesive in a DVD disc (see below) may prove to be preferred. One such adhesive is described in U.S. Patent No. 5,879,774, the disclosure of which is incorporated herein by reference. The patent application No. 09/263,972, filed by Virtanen, is also known by the name of "Monomolecular Adhesion Methods for Manufacturing Microfabricated Multilaminate Devices", which is hereby incorporated by reference. The manners mentioned are incorporated herein, presenting another alternative to standard adhesives, which can be demonstrated to be particularly advantageous for securing a laser refractive cover to a trackable optical disc having a non-operational structure. Ministry of Economic Affairs Intellectual Property Office Staff Cooperatives The printed polycarbonate is selected from the cover sheets exemplified herein to take advantage of the well-known optical alignment of the polycarbonate and the suitability of the device for its casting. However, other plastics can be advantageously used to create a disc cover. The plastic comprises polymethacrylic acid, polyethylene, polypropylene, polyacrylic acid, polymethyl phthalic acid ester, polystyrene, polytetrafluoroethylene, polystyrene, polycarbonate, polyacetal, Polysulfones, cellulose acetate, cellulose nitrate, cellulose acetate or a mixture thereof. Glass can also be used. As indicated above, the non-operational structure is preferably in a confocal configuration with the operational structure of the single data layer optical disc of the present invention. This situation allows the latter to focus on both the non-operational structure and the operational structure of the disc. Even when the signal element is straight-53-this paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 x 297 mm) 1247894 A7 B7 V. Invention Description (51 Road 2 Operations I: In-Building - This is an example In the disc, the tiger is maximized in the swaying pattern. The second and second will solve the non-operating structure and the operating structure (especially the tracking). In the focus plane of the kite, the non-operating structure can be satisfied. And the operation of the knot (please read the note on the back and then fill in the page) The structure is sufficient to confocal and the component of the disc reader is simultaneously detected. Therefore, the non-operating structure can be attached to the far side of the cover laser (also That is to say, the disc is close to it, not on the surface of the disc itself. This approach presents some significant advantages. First, the non-operating structure is configured to dramatically increase the chemical on the plastic to secure the non-operating structure, such as specific Analyte signal elements to the surface. Although gold-vulcanized binders have been shown to be widely adaptable - such as attachment of antibodies (example 2), attachment of cells (example 3), attachment of nucleic acids (example 7) - plastic presentation can be attached A wider choice of learning materials. Second, although the gold surface can be patterned to present the attached discrete lattice points, the plastic surface can even be more easily derived to present a chemical reaction group of stereo definition patterns. In turn, the application of non-operational structures in stereoscopically addressable patterns is enhanced. The Ministry of Economic Affairs, the Intellectual Property Office, the Staff Consumer Cooperative, prints some of these patterns and their advantages in co-owned and pending US patent applications. No. 08/888,935, filed July 7, 1997; and the application of the application Serial No. 09/120, 049, filed on July 21, 1998, the disclosure of which is incorporated herein by reference. The advantage discussed in this document is the ability to report patterns of analyte concentrations across a wide dynamic range to configure signal elements. 54 1247894 Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed A7 ------- Π7 —_ V , the invention description (52) &quot;&quot; a other stereo definition and stereoscopic bombardment with the ladder version of the addressing pattern is easy to establish these advantages themselves. For example, with other bases The above three-dimensional ^ &lt; upper body y-site 矽 type such as 矽 wafer or glass sheet configuration nucleic acid utilization is well known. Even, the specific analyte element placed near the inner diameter of the disc is read at the beginning of the disc action The specific analyte element placed at an inner diameter is read after the progressive rotation delay. In the unbalanced analysis, the pattern easily allows the power check to be performed, and the earlier reaction time point is thus The report of the fixed spot around the disc is configured. Third, the plastic surface can also be easily derivatized to exhibit the desired degree of hydrophilicity, which presents an advantage over the metal surface when the surface must be uniform to wet the water sample. In addition, the surface can be patterned into a hydrophilicity and a water-staining degree in which the region exhibits a degree of change. Fourth, we have observed that the flow of fluid across the surface of the disc is affected by the wobbled groove itself and by the groove itself. This situation is illustrated in Figure 38. The microbeads used therein are susceptible to alignment along the track. The flat surface of the disc cover does not exhibit such a barrier to a uniform flow of fluid across its surface. Fifth, the microfluidic component can be easily engineered into a plastic cover. The microfluidics are described in particular in the application Serial No. 09/064,636, the disclosure of which is incorporated herein by reference. Another advantage of configuring the non-operating structure on the plastic cover is that the readable optical disc itself can be reused when the cover is non-integral and can be removed by the optical disc assembly. -55- This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297 public Γ - ----------------- (Please read the notes on the back and fill in the form) Page) A7 B7 1247894 V. INSTRUCTIONS (53) The cover, whether integral or non-integral, removable or permanently fixed, with or without a non-operating structure disposed thereon, can function as other superior functions. It should be apparent that the reflective surface of the optical disc of the present invention is exposed to the air as opposed to the standard optical disc reflective layer 114. For this reason, non-oxidized metal is preferably used in its manufacture, although it is wrong or covered by a thin layer of plastic. The oxidized metal can be used. When exposed, the specific analyte of the first surface of the present invention determines that the reflective layer 134 of the disc is subject to wear, dust and the like, possibly attenuating the signals thus obtained. The plastic cover advantageously protects the reflective surface and Protect the information from environmental degradation. In addition, the cover serves to isolate infectious diseases and other vectors that cause disease, and a major benefit is the immunoassay of viral media such as HI V. The application of the removable cover 140 after the non-operational structure 136 has been disposed on the reflective layer 134 can compress the non-operating structure and drive it into the wobble track 138, further bringing the signal elements closer to the non-operating structure of the disc, To increase the signal. Polystyrene has proven to be particularly advantageous between plastics that facilitate the construction of laser-refractive covers: many current clinical tests are performed on polystyrene surfaces. Immunoabsorbance assays used in leaven chains Standard (ELISA) and radioimmunoassay (RIA) standard microtiter plates made of polystyrene. Participate in a large amount of experience in the clinical validation of polystyrene surfaces; It can be easily adapted to the presentation platform. In addition, precision casting of polystyrene is currently realized and easily completed. Even the cover can be advantageously applied by glass instead of plastic or other -56- paper scale China National Standard (CNS) A4 Specification (210x297 mm) --------^--------- (Please read the notes on the back and fill out this page.) Ministry of Economic Affairs Intellectual Property Office staff consumption Co-op printing A7 1247894 ___B7______ V. Description of invention (54) Substantially transparent ultra-cold fluid, liquid crystal or crystalline material containing niobium. It is understood that the thickness of the cover plate 140 must be adjusted to take into account the refractive index of the selected plastic. The difference is to focus the laser on the non-operating structure of the disc. This adjustment is taken for granted by the disc technician's skill. It will also be understood that the non-operational structure is detected via the cover plate. The surface rather than the first surface - will sometimes benefit from the presentation driven by the gain control circuit, as found in the large number of main features of the CDRW drive. Although illustrated as a consistent combination, the cover may also be surface patterned. There may be a non-uniform combination in the radial plane or the direction of the optical axis or both. It is also possible to achieve many advantages imparted to the plastic cover by arranging the non-operating structure by plating the reflective surface of the optical disc with a thin transparent layer of plastic, which is then applied to the cover (i.e., multiple layers in this layer). The most laser of the structure is close to the surface). Polystyrene resin can be easily used for this purpose and then in situ heat treatment. The resin is applied by a hollow configuration or a rotating coating, followed by UV photothermal treatment; this treatment is carried out in the related art as polymethacrylic acid, widely known as "2P" treatment. The latter approach gives the configuration of non-operational structures, such as the previously mentioned advantages of specific analyte signals on plastics. Even this method removes the boundary condition that otherwise exists between the cover and the gold surface of the disc, allowing the plane to be rotated by the more coordinated optical disc. However, because the layer is designed to be thin enough to place the signal element that is substantially confocal to the disc operating structure, the layer alone is not sufficient to fully assist focus. A suitable size cover can be used to make I!! — — ! — — I I I I I I I ! 11! -^ . (Please read the notes on the back and fill out this page.) Ministry of Economic Affairs, Intellectual Property Bureau, Staff Cooperatives, Printed Clothes

1247894

V. Description of the invention (55) The Ministry of Economic Affairs' Intellectual Property Office staff consumption cooperative prints that even the ruthenium coating can be advantageously composed of thin-layer glass instead of plastic or other substantially transparent ultra-cold fluid, liquid crystal or crystalline material (4). . As further discussed above, the additional lens 17 is allowed to be removed by the optical pick-up when the laser refracts the cover plate to the disc to establish a disc assembly that re-stors the second surface feature to the disc. The data presented in Fig. 4A was obtained using a disc manufactured as in Example 5 having a cover plate as manufactured in Example 6, and was read by a Rie〇h 6200S CD-RW disc drive without being assisted in Examples 2 and 3. An additional focusing lens that focuses. As shown in Fig. 31 and Fig. 32, the optical disc manufactured in the example 5 is used to generate the data shown in Fig. 40, and has a grain depth of approximately 1 〇〇 nm, which is close to the optimization of the first surface detection. Even with a cover plate, the 1〇〇nm grain depth allows for an off-the-shelf clock between the signal and the background, as evidenced by the electronic tracking shown in Figure 4, which shows that the construction is readable and non-operational. The current way in which a single data layer of a structure can be tracked is very robust. The cover 140 is selectively presented, and the verification grid is being prepared on the disc, and the sample is added; if it is desired to develop the inspection, another verification step is performed. Thereafter, in order to prepare the disc for reading, the cover 140 is placed on the reflective layer 134 and the signal element 136. Swing Detection As discussed above, the wobble track is a tracking structure that proves to be particularly advantageous for both the operative and non-operational signals in the single data layer embodiment of the present invention and for the ferrous detection. To allow users of the CD (and subsequent DVD) media to direct the recording. As stated in the relevant standards, the colloquial terminology is the Orange Book, which is written by the recording device to the CD-58- This paper scale applies to the Chinese National Standard (CNS) A4 specification (2W χ 297 public) --- ----Book---------Line (please read the note on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1244894 A7 B7 V. Invention Description (56) Period Detection; thereafter, the search is done by detecting such insufficiency. The specific optical disc device, which has been manufactured primarily for quality control purposes, has the ability to detect the wobble seek structure without having to nest into the disc. Examples of such devices include those manufactured by CD Associates of Irvine, Calif., and Audio Development AB of MalmS, Sweden. Therefore, in addition to the use of these specialized quality control devices, the standard and the existence of many standards are generally expected. The wobble is redundant after writing. Since the wobble is typically detected only during the nesting process, a process that does not require most of the fabrication of the present invention (and laser pulses that cause amplitudes to interfere with the detection of a particular analyte signal) is specifically designed for quality control purposes in CD manufacturing. The reader is used in Examples 2 and 3; the device detects and tracks the wobble without forcing the laser to pulse with the energy required for the disc to enter. The Ricoh 6200S CD-RW disc player, which does not require an additional focusing lens to produce the Figure 40 data, is adapted to read the wobble without writing to the disc. Signal Processing In Example 3, as briefly described above and as explained in detail below, the analog HF signal is fed to a digital oscilloscope to produce the instant traces shown in Figures 13-18. Conversely, Figure 40 is first captured as 'digitized, stored on a computer disk, and then displayed on a computer monitor only by interpretive software, further written by Worthington et al., 1999 8 The "Methods and Apparatus for Analyzing Nonoperational Data Acquired from Optical Disc" description of U.S. Patent Application Serial No. 09/378.878 (issue number BURST-15), filed on March 23, and filed by Worthington et al. US patent-59- applied at August 23, 1999. This paper scale applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) -------^--------- ^ .^^91 (Please read the notes on the back and fill out this page) A7 1247894 ___ '_B7__ V. Invention Description (57) The application number is No. 60/150-288 (The firm's case number is BURST- 18P) "Methods and Apparatus for Optical Disc Data Acquisition using Physical Synchronization Markers". 'The application document is hereby incorporated by reference. Multiple Data Layer Optical Discs A second series of embodiments of the present invention utilizes a multiple data layer structure developed in the recently developed Digital Diverse Disc (D V ) format. As discussed in detail below, the DVD format is particularly well suited for providing optical disc geometry, i.e., seek design, allowing both the disc seek signal and the signal generated by the non-operational structure to be simultaneously expanded and clocked. Referring now to Figure 24, a typical dual layer DVD format disc is shown. With the tradition here, laser light is incident from below. The optical disc includes a laser close to the substrate 282, a semi-reflective layer 284, a spacer layer 286, a reflective layer 288, and a laser transport substrate 290. The substrate 282 is coated with a transparent optical material such as polycarbonate having a selected index of refraction to assist in focusing the laser beam on one side of the second layer of data. The proximity substrate 282 can be fabricated by an injection casting process similar to that used above for fabricating CD-R format discs. The proximity substrate 282 is typically embossed with embossed material along the configuration of the spiral. These materials are typically considered to be present in the "layer" of a two-layer disc. The surface containing the substrate adjacent to the substrate 282 is plated with a semi-reflective layer 284, including very thin coatings such as ruthenium, gold, chains, silver, copper or alloys thereof for reflecting some light and transmitting some light. . Although the reflectivity can accommodate a range, the semi-reflective layer 284 typically has approximately 30% -60- of this paper size @目$鲜(CNS^4 specification (210 X 297 mm) -------- ^--------- (Please read the notes on the back and fill out this page) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperatives Printed 1244894 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperatives Printed A7 B7 V. Invention Description (58) Reflectivity. Thus, the semi-reflective layer 284 can have a reflectivity of about 20% to 40%, preferably 25% to 35%, and most preferably about 30%. The material of the poly-acidic acid can be fabricated by spiral data tracks. Since the laser beam will not pass through the remote substrate 290', its optical characteristics are not important. The remote substrate 290 can be manufactured by injection molding. As described above, the remote substrate 290 is embossed with the data in the spiral data, and the rail can be operated in parallel with the spiral data of the layer 0 (that is, from the inner portion to the outer portion of the optical disc) or The spiral data of layer 0 is in the opposite direction (that is, from the outer part of the disc to the inner part) The material embossed in the remote substrate 290 is considered to be present in the "layer 1" of the two-layer optical disc. The surface of the remote substrate 290 containing the data surface is mirrored by the reflective layer 288, including the thin layer. Materials such as gold, pavilion, silver, copper. Reflective layer 288 typically has a reflectivity that is designed to be close to layer 0. This mode can be accomplished by automatic gain control forced re-adjustment when reading from one layer to another. This change in gain can adversely affect the seek. For this reason, layer 1 of the dual-layer disc most often has a reflectivity well below 70%. Interval 286 provides a space between 40 and 70 microns in the double layer. Between layer 1 and layer 0 of the optical disc, and acting to bond the proximity substrate 282 and the semi-reflective layer 284 to the distal substrate 290 and reflective layer 288. The spacing 286 typically includes a refraction having a refractive index close to that of the material from which the substrate 282 is fabricated. Index optical adhesive. In use, the DVD reader can focus the laser beam on the side of the semi-reflective layer 2S4 to read the data in layer 0 or on the reflective layer 288 to read the layer. 1 in the information. More DVDs Features and DVD Reader Coexistence -61 - This paper size applies to China National Standard (CNS) A4 specification (210 X 297 mm) -------- order--------- line (please 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 5, Invention Notes (59) The focus is on making DVDs particularly well suited for use in the present invention: occupied by optical disc operating structures In these embodiments, the plane can be physically separated from the plane occupied by a particular analyte element, and the two types of data can be enhanced while being expanded. Thus, in one embodiment, the non-operational structure and the data layer are confocal; the optical tracking and other operational structures are located in the data layer 1. Conversely, in another embodiment, the non-operational structure and data layer 1 are confocal, and the optical tracking and other operational structures are located in the data layer. In another alternative, the assay element is disposed in the spacer layer 286 and is substantially confocal to one of the two data layers. Some of these principles are actually illustrated by reference to the specific embodiment shown in Figure 25. Fig. 25 is an exploded side view showing the DVD type double data layer verification optical disc 3〇〇. The disc is substantially detached along a plane defined by the spacer layer 286 in a typical double-president DVD disc. The optical disc 300 includes two parts: a main portion 302 and a cover portion 303. This portion may be permanently fixed to another portion, may be separated and combined, or may be combined separately and in reverse. In any of these constructions, the cover portion 303 is combined over the outer verification region 306 of the main portion 302 prior to the optical disc reading. The apertures 308 and 304 are sized to allow for a two-piece fit and reliable mounting. The external verification area 306 of the main portion 302 includes a single data layer area on which the non-operational structure is disposed. Similar to the single data layer embodiment presented above, the external verification region 306 is embossed with a wobble track (not shown), or other suitable tracking structure for use in providing a search for a syllabus-62- The paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). One -------------------- Order --------- Line (Please read the note on the back and then fill out this page) 1247894 A7 B7 V. Invention Description (6〇) (Please read the phonetic on the back? Please fill out this page again) Hunger to CD reading machine. In contrast to the DVT) standard, and in contrast to the single data layer embodiment presented above, the wobble pattern can be a forward image or a reverse image track. As mentioned, the ZCLV format can be used. The main portion 302 also includes an internal data area 3〇4. The internal data area 3〇4 is formatted in a manner similar to a normal dual DVD. Programs and materials can be stored in layers and/or layer 1 of this area. In particular, the meat portion data area 3.4 preferably includes a directing optical disc reader to adjust its focus to correct the data layer and to read a particular analyte signal present in the assay area 3〇6. In addition, the internal data area 〇4 can be stored to adjust the firmware or the driver chipset "flash, component data, as needed to allow the drive to properly read and interpret specific analyte signals. The plate SHAO 303 preferably includes a transparent optical material such as polycarbonate, polymethyl propylene or glass, and is selected to detect the optical structure of the optical disk (eg, wobble track) and signal element detection. Optimization. The printing of the Ministry of Economic Intelligence's Intellectual Property Bureau employee consumption cooperatives will obviously be that the change in the relevant skill and skill includes the inclusion of the non-operational structure to layer 0 or layer 1 of area 306, or On the second floor, the fetching structure is physically separated from the verification plane, which may itself lack a tracking structure or combination. In addition, the verification may be performed on the cover portion 303 by precipitating the signal element prior to the disc assembly. The laser distal surface is performed on the cover portion 303. It will be apparent to those skilled in the art that many minor variations can be made in this particular embodiment. For example, If a large amount of data or a program is required to interpret the verification result, the internal data area 304 can have data nested on layers 0 and 1 without changing the external verification area -63- This paper size applies to the Chinese national standard ( CNS) A4 size (210 X 297 mm)

1247894 306 layer 0 wobble track and verification result (ie signal element). Another set of advantages of the multi-layered DVD format can be understood by reference to Figure 26, which is a cross-sectional view of a verification optical disc in accordance with another multi-layered embodiment of the present invention. With the tradition here, laser light is incident from below. The optical disc 320 includes a channel 322 located in the spacer layer 324. The verification can be performed by introducing the material of the test through the opening 325 to the port 322, which is located at the far end of the disc laser. When the timing is performed, the signal element is disposed on the reflective layer 326 of the layer 320 of the optical disc 320. The layer 320 of the optical disc 320 is embossed with a wobble track to provide a minimum operational need for the optical disc reader. Layer 320 of the disc contains the data and programs needed to read the verification disc and interpret the results. &quot; For those skilled in the art, it is apparent that most assays can be performed on a single disc by using separate channels 322, each designed to handle different assays. Additionally, it will be apparent that the position of the channel 322 within the interval 324 can vary. For example, channel 322 can be adjacent to layer 〇 instead of layer 1, or can be roughly centered at interval 324. In one of these cases, the 'signal element' is placed in the overnight 322 to perform the verification result, which can be detected in a laser slewing path that is focused on the non-operational structure presented in layer 320 of the disc. The co-owned and pending trial application filed on April 21, 1998, with the serial number 09/064, 636, is hereby incorporated by reference in its entirety, indicating various different general and other three-dimensional verification CDs. Change. Figure 27 shows a side cross-sectional view of the optical disc 320 similar to that of Figure 26. The laser will be incident from below. In the optical disc 330 of Fig. 27, the overnight 322 is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) to the light-64-paper scale. --------tr----- ---- (Please read the notes on the back and then fill out this page) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperatives Printing Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperatives Printing Clothing 1247894 A7 B7 V. Invention Description (62) Positioning makes the central part of the disc 3 3 0 a "standard" layer 2 disc. The layer 330 of the disc 330 is divided into two sections. Layer 0 section 334 stores data or programs, as explained above. Layer 0 section 336 includes a transparent material having optical properties that may differ from the optical properties of section 334. In a preferred embodiment, the optical properties of layer 0 segment 336 are optimized to focus the laser beam onto the optical disc inoperative structure, as well as to optimize the signal elements in detection channel 322. Referring to Figure 2, another two-layer embodiment constructed in accordance with the principles of the present invention is shown in a similar cross-sectional view. The disc 340 can be used for a DVD reader or a CD-R reader. The disc 34 layer is configured according to the DVD format. The data encoded on layer 340 of the disc 340 can be read by a standard DVD player. The optical disc 34 layer 1 is coded according to the CD-R format, so that a wider track pitch and a low density configuration can be used. The data can be arranged in the central portion 342 of the disc 34 layer 1 . The verification portion of layer 1 is embossed with a wobble track to meet the operational requirements of the optical disc reader, and adjacent channel 346 for performing the verification&apos; as explained above. Figure 29 shows another embodiment of a two-layer optical disc. On the optical disk 35, the data and operation structure of the two-layer optical disk layer 1 are provided by the hologram 352. The hologram 3 52 is similar to the hologram 194 of FIG. 20, so that the operational structure and data encoded on the hologram 352 of the optical disc reader will be positioned on the image plane 354, which may be a laser relative to the hologram 352. The far end or the laser is close. Channel 356 is used to perform the verification, so the signal elements can be placed in spacer layer 358 of optical disc 35. These signal elements are detectable in the swivel path of the laser beam focused on the holographic 352 operating structure. For those who are familiar with the relevant art, it is obviously a holographic image similar to the full image 352-65- This paper scale applies the Chinese National Standard (CNS) A4 specification (21〇x 297 mm) I------ --- — — 1 set —1111--• line (please read the note on the back and then fill out this page) 1247894 V. Description of the invention (63) to provide layer 1 in a few ü, medium, 7 you cloth Brief description &lt; 任 _ double-layer disc. As shown, the shadow i 丫 354 relative to the whole male q Zhi &lt; · (please read the phonetic on the back? Please fill out this page) will be out of the gfrag &amp; winter 35 ▲ surface is close to the laser, so the signal element Swing inside the groove. On the surface of layer 1, or in the simulation of the hologram, the person familiar with the relevant art is obviously referring to the two-layer pupil display (the specific embodiment can be easily extended to use With: more than two layers of multi-layer discs. For example, 'the channel used to perform the verification can be: between the layers of the multi-layer disc of the scholar, and each layer (except the layer) provides any operational structure required by the disc reading machine. The following examples are provided by explanation and not by way of limitation. Example 1 Manufacture of track-ready forward-image embossed inverted discs suitable for specific analyte verification. Cinram (Anahan, Calif.) manufactured with CD_R format The unpunched father of the Taoist image is matrixed into a 〇_11 master by standard procedures. Simply, the electroforming is done by Digital Matrix (New York)

Execution in a nickel sulfamate tank in an electroforming system manufactured by Hempstead. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumers' Cooperatives. The masterbatch is cleaned, polished, and punched, and then directly used as a stamper to make an inverted disc with an operational structure that is embossed to the image spiral track. The NETSTAL casting machine manufactured by Netstal Machines (Sweden) and the CD-R mould created by AWM Sweden are used to produce discs in EXIMPO S.R.〇 (Prague, Czech Republic). The casting parameters of the injection casting machine are adjusted to enhance the high degree of ventilation in the mold, while the -66 paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). A7 1247894 B7 V. Invention Description (64) Correctly Regenerate the grain. The polycarbonate used to mold the optical disc was produced by Bayer Plastics. (Please read the note on the back and fill out this page.) The polycarbonate disc is then metallized with gold using a metallization tool made by First Light Technologies (Saco, Maine). As shown in the AFM measurements in Figure 10-12, these discs have a depth of approximately 170 nm and a magnetic pitch of approximately 1.6 μm. Example 2 Construction of an IgG-specific immunoassay grid on a searchable disc A single data layer, a first surface, a forward image/positive embossed oscillating disc was manufactured according to Example 1. The gold surface of the disc is then derived as follows to construct a test spot specific to the disc to detect human IgG in the blood sample. 2 mg N-[6-(vitaminium amino)hexyl]-3'-(2'-pyridylperthio)propanamide ("Vitamin H_HPDP") (Pierce, Rockford, IL; Art No. 97032461) The component is dissolved in 2 ml of dimethylformamide. At each of the four intent checkpoints, each was positioned at the same radius from the center of the disc, and 10 μL of the vitamin H-HPDP solution was pipetted. The disc was incubated at room temperature for 2 hours and then washed with a 50 mM phosphate buffer (pH 7). Printed by the Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperatives Next, 10 μΐ of streptavidin solution (Monobind, Costa Mesa, CA; product 96-001/MF; 2 mg/ml) was pipetted at the same test point. The disc was incubated for 1 hour at room temperature and then washed with a 50 mM phosphate buffer.

Biotmylated goat anti-human IgG by Chemicon International -67- This paper size applies to China National Standard (CNS) A4 specification (21〇X 297 public) 1247894 A7 B7 V. Invention description (65) (Temecula, California; affinity purification ., goods 47797017) obtained. The 5 μΐ (please read the precautions on the back and fill out this page) can be moved by pipettes at each of the four test grid points. The optical disk was incubated at room temperature for 1 hour, and then washed with a 50 πι 11 1 acid buffer (pH 7). The geometry of the completed checkpoint is illustrated in Figure 7A. Vitamin H-HPDP 70 forms a molecular layer above the surface of the disc and is bonded to the disc gold surface (An) with a gold-vulcanized and lattice (coordinate) adhesive. Streptavidin 72, each molecule can bind 4 vitamins and molecules to form a sublayer with high affinity. Biotinylated goat anti-human IgG 76, which confers specificity on the assay grid, after which, in particular, the vitamin Η is divided into 7 to the fixed streptavidin 72. The goat anti-human IgG is biotinylated at a position that allows it to be immobilized without interfering with antigen (human IgG) binding. The disc thus derived is used to verify the presentation of human blood IgG. A sample of 100 μΐ human blood was drawn from normal volunteers. The 100 μΐ sample can be diluted 10 times with a phosphate buffer base (“PBS”). Two additional 10: 1 series dilutions are performed identically. Each of the same 10 microliters (μΐ) component - that is, the fraction of undiluted blood and the component of the 3 series of diluted blood samples - is separately and separately placed in one of the 4 disc verification points. Fixed point. Printed by the Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperative. The disc was incubated at room temperature for 2 hours under nitrogen in a closed humid chamber. The disc is then washed with PBS. To develop an IgG-specific assay, that is, to make it suitable for reporting the presence of IgG in a sample of the application, 5 μΐ (160 μβ) of MagaBeadsTM goat anti-human IgG (Fc) (Cortex, San Leandro, CA) Biochem Male-68- This paper size is applicable to China National Standard (CNS) A4 specification (210 297 297 mm) 1247894 A7 B7 V. Invention description (66) (Please read the note on the back and fill out this page) 7A2201A) was marked as a mark at each of the 4 test grid points, and the disc was washed with a 50 mM phosphate buffer (pH 7) and then washed with distilled water. The geometry of the assay grid after IgG capture by blood and development with anti-human IgG MagaBeadsTM is illustrated in Figure 7B. IgG 78, which has been presented in a blood sample (analyte), was ligated by biotinylated anti-human IgG 76 and immobilized on the assay site. The human IgG 78 further functions to immobilize anti-human IgG MagabeadsTM 79 ° MagabeadsTM for commercially available spheroidal latex magnetizable particles - and one of a variety of different types of junctions, such as here goat anti-human IgG or another And pre-consume with a reaction group that allows custom conjugates. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumer Cooperatives. The disc is dried and its surface is visualized by light and atomic force microscopic (AFM). Figure 8 is a video image of a light microscopic view capture of a disc that can be tracked by a portion of an IgG-specific first surface-specific analyte after addition to human blood and antibody-consumable spheres. Figures 9 and 1 show an AFM image of a single latex sphere that is specifically attached to the disc, the magnification being slightly higher than the magnification used in Figure 8, and the measured foot size as observed by AFM during image capture. Figure 11 is an afm image that is immunologically attached to the first surface to search for human IgG-specific discs and two latex spheres present in the same field, and summarizes the measured size observed by AFM during image capture. Example 3 Electronic detection and characterization of human red blood cells on RBC-specific searchable immunoassay discs Single data layer, first surface, forward image/positive relief oscillating disc is based on -69- This paper scale applies to China Standard (CNS) A4 specification (210 X 297 mm) Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 1244894 A7 B7 V. Invention Description (67) Manufactured according to Example 1. The gold surface of the disc is then derived as follows. 2 mg N-[6-(vitaminium amino)hexyl]-3f-(2'-pyridylperthio)propanamide ("Vitamin H-HPDP") (Pierce, Rockford, IL; Art No. 97032461 The component can be dissolved in 2 ml of dimethylformamide. At each of the four intent checkpoints, each was positioned at the same radius from the center of the disc, and 10 μL of the vitamin H-HPDP solution was pipetted. The disc was incubated at room temperature for 2 hours and then washed with a 50 mM phosphate buffer (pH 7). Next, 10 μΐ of streptavidin solution (Monobind, Costa Mesa, CA,·product 96-001/MF, · 2 mg/ml) was pipetted at the same assay point. The disc was incubated for 1 hour at room temperature and then washed with a 50 mM phosphate buffer. Monoclonal mouse anti-human glycophorin A antibody (added to Carkinteria City Dako; product 113) was biotinylated as follows. 100 μΐ of antibody can be combined with 0·1 mg of vitamin Η, 0-Ν-mercapto dimethyl amide (polyethylene glycol)-carbonate ('' Bio-PEG-NHS,,) (AL Shearwater, Huntsville, State; PT-028-27) is a 100 μl phosphate buffer (pH 7) and allows for 1 hour of reaction. Vitamin η - conjugated anti-human glycophorin Α was dialysis overnight compared to the same buffer (dialysis MWCO = 30,000). The dialyzed vitamin Η-conjugated anti-human glyCOphorin A antibody was pipetted on a streptavidin-plating test spot on a disc and after the disc was incubated at room temperature for 1 hour, after which a 50 mM phosphate buffer was used. (pH 7) was washed. -70- degree is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) „ -------- order--------- line (please read the notes on the back and fill in again) This page)

A7 1247894 _____B7______ V. INSTRUCTIONS (68) The disc thus derived is used to verify the appearance of red blood cells in human blood. A sample of 100 μΐ human blood was drawn from normal volunteers. The 10{) μ1 sample can be diluted 1 〇 times with phosphate-buffer base salt ("PBS"). Two additional 10: 1 series dilutions are performed identically. The i 〇 microliter (μ1) component of a sample and the 10 μΐ component of each series of diluted samples are placed separately and separately on the 4-disc test grid. The disc was incubated at room temperature for 2 hours under nitrogen in a closed humid chamber. The disc is then washed with PBS. Figure 12 is an atomic force microscopic image of the atomic force that determines the RBC immune specific attachment to the disc. As indicated by the quantitative analysis, the RBC level was set to be 7.984 μιη, which was consistent with the known diameter of the red blood cells (8 μπ1); this size was significantly different from the 3 μιη diameter of the latex sphere used in Example 2 and observed. The RBC height above the bottom of the track was observed as the disc was cleaned with a 5% solution of triol, which was dried and read in an optical disc as follows. A CD-R device manufactured by CD Associates, Inc. (Lrvine, Calif.) for use in optical disc quality control is used to read the disc. The CD-R wobble tracking system (mode RSL100) of the drive unit is incorporated into the lens 17 to correct the optical pickup to adjust the focus when the first refractive layer is lacking on the optical disc; the height of the rotary shaft is also increased. The H F signal (R F or the sum of the four columns) is amplified by an electronic circuit in the RSL 100, and the buffered HF signal is input to a digital oscilloscope. Figure 13 presents a representative trace showing the time on the X-axis and the four-column sum signal size on the γ-axis. Figure 1 3 shows that the red blood cells are directly visible as _ -71 - this & Zhang scale applies Chinese National Standard (CNS) A4 specifications (210 X 297 mm) &quot;&quot;&quot; -------- Order ---------Line (please read the note on the back? Please fill out this page again) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1247894 A7 B7 V. Invention Description (69) (Read first Note on the back side of this page) CD-R reader η F signal high-frequency, high-amplitude events; for mammalian cell size analytes, there is no latex sphere or other external signal is part of the signal It is necessary to generate a specific analyte signal. It is also apparent from the oscilloscope trace of Figure 13 that the difference in the HF baseline is a double peak. Although red blood cells are known to have a characteristic biconcave shape, we have observed in Example 2 that this double peak is used to report the presentation of the analyte. When this double peak appears as a sphere in the traversal of the track by the laser, it is formed by the &lt; regeneration change in the reflectance. According to another observation of the oscilloscope tracing of Fig. 13, it is easy to see that the reference line which is inverted on the signalling event is stable; that is, the wobble track (which is manufactured as an inverse image wobble track) The tracking itself does not cause significant changes in the guad sum signal. Optical inter-readers such as the CD-R standard maintain a constant linear velocity regardless of the position being read on the disc, correcting the spindle speed to lock the frequency of the wobble track. Based on the known linear speed of the disc and the incremental indication on the oscilloscope trackdown, the divisions on the oscilloscope search can be displayed as equivalent to a linear distance of 13 μηχ on the disc. As measured by the oscilloscope trace shown in Figure 13, the difference in the four-column sum signal reference line is therefore set to 1 〇 μπι, which approximates the non-corrected size of the object in the direction of the tracking track. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumer Cooperative of the Ministry of Economic Affairs. The actual size of the object is small. Reader calibration and oscilloscopes using 3 μιη latex spheres have given the oscilloscope a peak report of 5 μπι, which is 2 μηη wider than the actual object. This situation is appropriately considered to be a 1·5 μχη laser focus diameter on the first surface on the verification optical disc. Consider the actual size of the measurement and the diameter of the laser on the surface of the disc -72 - This paper scale has been printed with the Chinese National Standard (CNS) A4 specification (21〇X 297 mm) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative System (1) 8h 1247894 Α7 ____ Β7 5, invention description (7〇) When the difference between 2 μηι, the event captured by the oscilloscope in Figure 1 is the high-frequency, high-amplitude difference of the four columns of the sum signal, reporting 8 μηι objects Size, and known 8 μιη human red blood cells. Figure 14 presents another oscilloscope homing to the H F event detected by the isolated red blood cells on the same disc. Biphasic guidance is more pronounced. Figure 15_17 is an additional example. Figure 18 is a digital overlap of most events amplified from different areas on the same disc, actually illustrating the reproducibility of size and shape measurements on different red blood cells that are specifically attached to the disc. Example 4 Calculation of Optimal Signal Element Size A particular analyte signal can be optimized by adjusting the size of the sphere signal element relative to the size of the search pattern, as explained below. Figure 30 illustrates the calculation of the size of the sphere signal element 360 of the fit to the track on the disc 362 such that the signal element is joined to the pattern at 3 points: 1 point at the bottom of the track and 1 point at each edge of the grain. In the following equation, r is the radius of the sphere signal element, w is the groove width, and h is the groove depth. With the Pythagorean theorem, the relationship between the radius of the sphere and the width and depth of the groove is: jL·r is equal to (2) Since the groove depth is preferably λ / 8, where λ is used to read the disc. The wavelength of light can be expressed as: -73- This paper scale applies to China National Standard (CNS) A4 specification (21〇χ 297 mm) ---r------------- ----Book---------Line (please read the note on the back and then fill out this page) (3) (4) 1247894 A7 B7 V. Invention Description (71) 4 —+ w2 Simplify this The formula is equal to: 1 w2 r 2 - + - 16 , 1 Apply equation (4), if the wavelength of the light reading the disc is 0.65 μηη (that is, 650 nm, the wavelength is for DVD), and the width of the ridge For 〇·8 μιη (the magnetic pitch of the DVD), the radius of the spherical signal element must be approximately 1.03 μιη. Example 5 Optimized for the single surface with the forward image/positive embossed wobble track Fabrication of Layered Discs CD-R masters are produced at CINRAM, essentially as described in Example 1, to produce a track-searchable, single data layer, forward image/positive Carved groove wobble of the disc. The master was used to punch approximately 5,000 sheets of polycarbonate discs, which were then metallized with gold and stored for subsequent use. The disc was cast in EXIMPO S.R.O. (Prague, Czech Republic). The mold setting is stated in Figure 41Α-41Ι. The discs produced in Example 1 (and used in Examples and Example 3 to produce the data presented in Figures 8-18) have grooves with a depth of approximately 170 nm, reported as "vertical distance" summed up by the size provided by the atomic force microscopic. , reproduced in Figure 1 〇. Conversely, the disc manufactured here is designed to have a groove depth approximating the first surface and is theoretically optimized to be 1/8 of the intended incident laser wavelength of 780 nm. Figure 31 shows the atomic force microscopic of one of the disc inner diameters. ___ -74- produced by the inspection is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) * -------- order --------- line (please Read the notes on the back and fill out this page.)

Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 1247894 A7 -------BT^ V. Invention description (72) Information. The size summary report is about 1 〇〇 nm in depth. Figure 3 2 shows the data generated by the atomic force microscopic inspection of one of the outer diameters of the optical disc; as is typical of optical disc manufacturing, the groove depth is slightly larger than the outer diameter (here, 1〇1·23 nm) to accommodate the optical disc in the light. The outer portion of the axial direction is slightly increased in operation. Figure 3 3 shows similar data from the atomic force microscopic examination of the inner diameter of the master wafer, and Figure 3 shows the data generated by the atomic force microscopic examination of the outer diameter of the master. Example 6 Laser-Reflective Polyurethane Cover Plate Manufactured Laser Refractive Polycarbonate Cover Plate, one of which is shown in Figure 35 and in Figure 19 in a reversed section view (combined into a single data layer specific The Analyte CD is further illustrated and manufactured as follows. Nickel discs are intended for standard CD manufacturing, but are used for effective mounting on cast 4 machines and are placed in standard stamper polishers. The data surface of the ink mold is smoothed and polished to produce a stamper having a thickness of about 26 〇 μηι - 330 μηη with two polished faces. The stamper is mounted in the standard c〇-R and is set to adjust the polycarbonate cover to be approximately the same thickness, but on the other hand the size is equal to the standard 120 mm disc. The single data layer disc manufactured in Example 5 was approximately 12+/_ 〇〇 5 mm thick; the cover was approximately 1.17 mm thick. The two are added to create a component that is approximately 24 mm thick, and the maximum external solid thickness is provided by the Red Book standard (11 _ 1.5 mm for all layer combinations). Experimentally, we have found that the increased thickness of the disc assembly does not present optical or mechanical problems. The material presented in Fig. 40 was obtained using the optical disc manufactured according to Example 5 and combined with a cover plate manufactured according to this example before reading. The cover provides sufficient auxiliary -75- paper size for the Chinese National Standard (CNS) A4 specification (210 X 297 mm) ^ -----------------^ (Please Read the back note first and then fill out this page) 1247894 A7 B7 V. Invention Description (73) Help focus to exclude another focusing lens 1 7 Add to the optical pickup of the drive unit (please read the back note first and then fill in the book) Page) Sounder. Example 7 High Density Nucleic Acid Sequence Drive of Signal Elements Attached to a Definable Disc A single data layer, a first surface, a forward image/positive embossed oscillating disc was fabricated in accordance with Example 5. The disc gold surface is derived as follows. The transfer is performed in a layer of fluid in the dust-free stem. A single point of 15 pL of streptavidin solution (2 mg/mL) was placed on each of the 6 discs. The disc was incubated for 1 hour and then rinsed in a stream of distilled H20 (dH20). A 10 μί 2-hydrothioethylamine (137 pg/mL) component can be added to each point to block non-specific bonding (the solution has been stored at 4 ° C for a period of time sufficient to oxidize the solution). The disc was incubated for 3 minutes and then washed with dH20. The disc is not dried before the week. The nucleic acid probe was modified with an amine 3' or 5' bound column to be ordered by Keystone Laboratories (Foster, Calif.) as follows, 5, TCGGGTGTACTCAO Amine-3, (SEQ ID NO · 1) 5, TCCAAGAAAGGACC- 3, (SEQ ID NO: 2) * Each probe was then independently conjugated to vitamin Η via an amine-corrected binding column as follows. Ministry of Economic Affairs, Intellectual Property Bureau, Staff and Consumers Cooperative, printed material solution of vitamin H_PEG_NHS (penta-vitamin, ω_Ν-mercapto dimethyl carbamide poly(ethylene glycol)-carbonate) (MW=3400, Alaba Shearwater, Inc., Huntsville, MA; PT-028-27) by dissolving solids in a phosphate buffer containing azide as a preservative (PBSAz, pH 7.45) to a final concentration of 23·5 ηιηοΐ/μί (4.7 mg vitamin-76- This paper scale applies to Chinese National Standard (CNS) A4 specification (210 297 297 mm) A7 1247894 ___B7_ V. Description of invention (74) H-PEG-NHS at 58·82 μί). The 3, active probe (SEQ ID NO: 1) ("3' probe") was dissolved in PBSAz to a final concentration of 1 ηπιοΐ/μί (473 nmol at 473 μί). The 3' probe 20 (20 moi) solution was then added to a 10 μί vitamin H-PEG-NHS round solution equal to a final nucleic acid concentration of 660 pmol/pL. At the same time, the 5' aminated probe (SEQ ID NO: 2) ("5' probe") was dissolved in PBSAz to a final concentration of 2 nmol/μΙ^ (84 nmol at 42 μί), and 5' probe ΙΟμΕ (20 mol) The solution was then added to 10 μL of vitamin H-PEG-NHS equal to a final nucleic acid concentration of 1 nmol/pL. The solution was incubated at room temperature (RT) for 2 hours. The 3 f probe is then partially conjugated to the surface of a single dispersed phase superparamagnetic bead s (with a diameter of 2·8 μιη) via a vitamin Η moiety. 10 consecutive 10 1 : 10 dilutions prepared as above, 3, biotinylated probe (1 : 10 dilution of 66 pmol) can be added to the streptavidin-coated Dynabeads® (New: Johor 1^^^811 (^€ 35 City 〇5^仙1 company; the species number is \&gt;[-280#) 111^ component (100 pL, 10 mg/ml). The culture is at room temperature for 10 minutes and is between the first and second The probe solution was added to _beads performed between 5 minutes and between 2 and 3 minutes and 2 minutes between the other increments performed between 0 3 'probe conjugated beads followed by 200 PBSAz and The 200 μί hybrid buffer was washed twice (923 mM Na2HP04, 75 mM NaH2P04, 1 mM EDTA, pH 7.34). The beads were magnetically separated (Dynal) and quantified by the cleaning solution. Beads were mixed at 1 mL. Resuspend in the buffer. The nucleic acid is fully conjugated and presented in the conjugated bead solution. The average probe concentration is 198 fmol/ML. -77- This paper scale applies to the Chinese National Standard (CNS) A4 specification ( 210 X 297 mm) r --------^---------^ (Please read the notes on the back and fill out this page) Property Bureau Staff Consumer Cooperatives Printed Economy Ministry Intellectual Property Bureau Staff Consumer Cooperatives Printed 1244894 a7 ___B7_ V. Inventive Note (75) 5' biotinylated probe (1 nmol/gL is 20 pL) diluted with a hybrid buffer 1 mL (final concentration 20 pmol/pL) ° The target nucleic acid is synthesized by Keystone Laboratories and is expected to be as follows: 5,-GTGAGTACACCGGAATTGCCAGGACGACCGGGTCCTTTCTTGGA-3, (SEQ id NO : 3) The target is dissolved in PBSAz to a final concentration of 100 μΜ. Thereafter, 4 A series of l, 〇〇〇-fold (1 pL to 1 mL) dilutions were performed with a hybrid buffer to form target test solutions at concentrations of 100 nM, 100 pM, 100 fM, and 100 aM. The reaction is set in a parallel solution. In the &amp; reaction, 2 jiiL bead-conjugated 3, probe (396 f-mole nucleic acid) and 1 μl&gt; biotinylated 5, probe (20 ρ mol) to target DNA (SEQ ID Ν〇: cultured as follows: reaction 1 1 μί 100 ηΜ target = 100 f molar target reaction 2 1 μί 100 ρΜ target = 100 a molar target reaction 3 1 μί , 100 fM target = 100 z mole aims Reaction 4 1 μί 100 aM Target = 100 y Mohr Target Reaction 5 1 μιη Hybrid Buffer (Control) Reaction 6 1 μΕ Water (Control) Each tube was incubated for 2 hours at room temperature on a 300 RPM stirrer. After the completion of the hybridization, each of the reactions in the 6 hybridization reaction was washed twice with 10 &lt;) pL PBSAz, and the beads were resuspended in 10 jliL PBSAz by quantification of the cleaning solution. For each of the reactions in the 6 reaction, 2 μί of the bead suspension is applied to the lining r &amp; point on one of the discs to form the target total amount as follows: -78 This paper scale applies to the Chinese National Standard (CNS) A4 specification. (2〗 297 297 mm) K· I ϋ ϋ -II ϋ ϋ · I nn ϋ I ^1 I ^1 ^1 ^1 ^1 I ϋ n I (Please read the notes on the back and fill out this page 1247894 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed five, invention description (76 CD 1 20 f Moer (20 χ 1 (Γ 15 Moer) target disc 2 20 a Mo (20 X 10-18 Mo ) Target Disc 3 20 z Moer (2〇χ 〗 (Γ 21 Moer) Target Disc 4 20 y Moer (2〇χ 10·24 Molar) Target Disc 5 〇 (Hybrid Buffer Control) Disc 6 〇 (water control) rate beads are incubated on the disc for 1 , minutes, then washed with water. The disc is dried and then visualized using a light microscope. Figure 3 6 shows the grid at visual time. The gold surface of the track disc is the coating of streptavidin, which is formed by striavid gas and streptavidm protein. The vulcanized-gold binder between the free sulfyhydryl and the disc gold surface is combined. The streptavidin captures 5, the vitamin Η part of the probe. The 5, probe is in turn supplemented by 14-nucleonic acid Waison-Cdck at the target 3 f The end captures the target nuclear order. The target is sequentially captured at the 5th end by the Watson_Crick of the 14-nucleotide to capture the 3, end, thus tethering Dynabead® to the disc. Figure 3 7 shows the light taken by the verification disc j-3 respectively Microscopic image, each disc has a magnification of 2: the attached sphere and the wobbled track can be clearly seen in all 3 images of the higher magnification panel. The increased number of attached beads can be clearly seen with an increased number of nucleic acid targets, while Disc 3 (Fig. 37C) shows that the sphere is supplemented with 20 gigaflops (20 X 1 〇 21 mol; 12 X 1 〇 3 mol) nucleic acid target-drive attached to the disc surface, while disc 2 (Fig. 37B) ) shows that the sphere is supplemented with a 20-billionth molar (20X10-18 mol; 12ΧΪ06 mol) nucleic acid target-drive attached to the disc surface, while disc 1 (Fig. 37A) shows the sphere with 20 -79- paper The scale applies to the Chinese National Standard (CNS) A4 Grid (2] 0 χ 297 mm) r r -------- --------- order line (please read the back of the note Pou? Matters then fill out this page)

1247894

V. INSTRUCTIONS (77) One-billionth of a mole of 莫^^Moule; 〖2χ〗 〇9 Moer) The nucleic acid target is supplemented and driven to the surface of the disc. There is no beading in it - the control disc (not shown) is observed on the surface. All of the patents, patent publications, and other published documents referred to herein are hereby incorporated by reference in their entirety in their entireties as if they are incorporated herein by reference. The preferred embodiments of the present invention are to be understood by those of ordinary skill in the art, and it is obvious that various changes and modifications can be made without departing from the scope of the invention, and All such variations and modifications are intended to be included within the spirit and scope of the invention. (Please read the notes on the back and then fill out this page.) Ministry of Economic Affairs Intellectual Property Office Staff Cooperatives Printed Moderate Ruler Paper 21 /V Grid 4) AS) N (C 准标家国一国80 * 公97 2

Claims (1)

1247894 Patent No. 0881 i8794 Patent Application Replacement of Chinese Patent Application (Summer of 94) VI. Patent Application Range: • Suitable for and with spiral or concentric information magnetically formed on the first surface of the optical disc The optical disc player of the optical disc combination operation, the magnetic track is formed to have at least an operation structure to provide a physical index suitable for the generation of the focus error signal and the tracking error signal generation. The (four) structure of the information track is an unidentified analyte structure of the confocal positioning, the player comprising: an optical device for generating a laser beam suitable for illuminating the information track; and a disc support device for supporting the optical disc Rotating with the laser beam; the port is for focusing the laser beam on the information track when the disk is in rotational engagement with the laser beam and for maintaining the focus servo on the information track a device comprising: a speed servo control device for controlling the rotational speed of the optical disk in a predetermined manner; including, for example, laser beam illumination a device for controlling a tracking servo for positioning the laser beam when rotating the information track; an output connector device for forming an image signal that can be used for connection to an external device; the optical device further comprising at least two a mode of operation of the detector device; the first mode of operation is for generating a signal suitable for application to the tracking servo I and the focus tracking servo; the first mode of operation is still used to generate an application suitable for use For the decoding of 61090-9401I2.DOC, the paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). 1247894 A8 B8 C8 _______ D8_ VI. Application for Patent Park to store the information on the information track. The signal of the device; the second mode of operation is used for transiently chopping the signal suitable for the tracking device and the focus tracking servo; and the second mode of operation is still used for connecting at least one of the four columns to read Signals are sent to the output for detecting the analyte structure positioned on the disc and characterizing the analyte structure. 2. The optical disc player of claim 1, wherein the tracking device comprises a radial tracking device and a tangent tracking device. 3. The optical disc player of claim 1, wherein the speed control signal is applied to the utilization device for spatially identifying the laser beam illumination point if the detected signal is applied to the utilization device. 4. The optical disc player of claim 1, wherein the utilizing device stores a repetitive scan of the analyte structure for storing information identifying the analyte structure. 5. The optical disc player of claim 1 of the patent application further includes a tracking error signal for detection and characterization of the analyte structure carried by the optical disc. 6. An optical disc player as claimed in the scope of the patent application, further comprising a focus error signal for detection and characterization of the analyte structure carried by the optical disc. 7. The optical disc player of claim j, wherein the optical device and the focus servo are used to focus the laser beam on a plane shared by the analyte structure and operation to allow operation and parallel analysis of analyte data. And the discriminable extraction, and the operation structure is formed on the disc Ray-2 - 61009.940112 D0C This paper scale applies the Chinese National Standard (CNS) A4 specification (21〇X 297 public Chu) ----- - I247894 A8 B8 C8 ____ D8 VI. Apply for a patent to shoot one of the tracks near the side surface or one of the wobbled tracks. 8. The optical disc player of claim 7, wherein the operation structure is one of a forward or reverse swing track. 9. The optical disc player of claim 7, wherein the operational structure is one of a forward or reverse standard optical disc (CD) information track. An optical disc player for operating in combination with an output utilizing device and a compact disc, the optical disc being formed as an information track having one of a spiral shape or a concentric shape generally formed on a first surface of the optical disc, The information track is formed to have at least some operational structure for providing a physical index suitable for at least focus error signal generation and radial tracking error signal generation, the optical disk having a confocal location with the operational structure forming the information track An unidentified analyte structure, the player comprising: optical means for generating a laser beam suitable for illumination on the information track, the optical device comprising detector means for generating at least one focus error signal (FE ), a radial tracking error signal (TE) and a high frequency read signal (RF, HF, four column sum); a disk support device for supporting the optical disk in rotation relationship with the laser beam; including a focus servo device and The optical disc is used to focus the laser beam on the information track and to maintain the focus on the information magnetic field when the laser beam is a rotating nozzle a device for generating the focus error signal by the detector device; a device comprising a speed servo control device for controlling the speed of the optical disk in a predetermined manner; 6J009-940112.DOC „ 3 - The paper scale is applicable to the Chinese national standard (CNS) Α4 size (210X297 mm). 1247894 A8 B8 C8 D8 -4- 6. The patent application scope includes a radial tracking servo device and the detector device when the laser beam is irradiated on the rotating information track. Generating means for controlling the radial tracking error signal for radial positioning of the laser beam; and output means for forming at least the RF signal for use as an image signal for connection to the external utilization device, The detector device has at least two modes of operation, the first mode of operation being used to generate a signal suitable for application to the radial tracking servo and the focus tracking servo, the first mode of operation also being utilized to generate an application suitable for the application The signal to the signal decoding device for decoding the information stored on the information track, and the second mode of operation for the transient chopping is suitable for a signal for the radial tracking servo and the focus tracking servo and for connecting at least the read signal to the output end. 11. The optical disc player of claim 1, wherein the first operation The mode is still used to generate a signal suitable for application to a signal decoding device for decoding information stored on the information track. The optical disc player of the first application of the patent scope is also included for the sum of the four columns. (HF, RF) signal separating the tracking signal for allowing the four-column sum signal to be used for detecting the signal by the analyte. The optical disc player of the first aspect of the patent application, wherein the optical device The apparatus for focusing the laser beam on the information track focuses the laser beam on a plane shared by the operation and the analyte structure for allowing parallel operation of the operational data and analyte data and for discriminating the capture. 14. If you are applying for the CD player of the scope of patent application, the operation mode is 61009-9401J2.doc. The paper size is applicable to the Chinese National Standard (CNS) Α4 specification (210X297 mm). Binding 1247894, the patent application garden is to form the physical structure of the information track on one of the surface of the optical disc. 15. The optical disc player of claim i of the patent scope, further comprising means for characterizing the output signal generated by the sum detector when the laser beam traverses the analyte structure, so The characterization device detects and displays the amplitude variation of the HF signal, and the duration during which the reflected beam is reflected across the analyte signal for providing a substantial qualitative amount of the analyte sensation size and construction. Measurement. 16. A disc player adapted to operate in combination with an output utilization device and a disc having one of a spiral or concentric shaped information track formed on a first surface of the optical disc, the magnetic track being formed to have at least An operational structure for providing an entity index suitable for at least focus error signal generation and radial tracking error signal generation, the optical disk having an unidentified analyte structure that is confocal positioned with the operational structure forming the information track, The player includes: an optical device for generating a laser beam suitable for illuminating the information track and the analyte structure; a disc support device for supporting the optical disc in a rotational relationship with the laser beam; a focusing device for focusing the laser beam on the information track and for maintaining the focusing servo on the information track when the optical disk is in rotational engagement; and including for use in a pre-determined manner a speed control device for controlling a speed servo control device of the optical disc speed; comprising information magnetic such as a laser beam irradiated on the rotation When used to control the scale 61009-940112.DOC this paper applies China National Standard (CNS) Α4 Specifications (210 X 297 mm). Binding 1247894 A8 B8 C8 -----:__ D8 6. Patent application for the tracking device of the radial tracking servo for radial positioning of the laser beam; for forming an image that can be used for connection to an external device An output connector device of the signal; the optical device further comprising a sum detector device having at least two modes of operation, the first mode of operation for generating a suitable approximation to the radial tracking device and the focus tracking servo a signal of the device, and the second mode of operation is used for transiently chopping a signal suitable for the radial tracking service and the focus tracking servo and for connecting at least the read signal to the output connector device Means for characterizing an output signal generated by the sum detector when the laser beam traverses the analyte, the characterization device for detecting when the laser beam traverses the analyte signal And displaying the amplitude change of the HF signal and measuring the duration of the reflected reading beam across the analyte signal for providing the analyte size and the essence of the construction Qualitative measurement. Means for separating the analyte signal generated by the second mode of operation by the operation signal generated by the first mode of operation, the separating device responding to the operation signal and for switching the detector device from the first mode of operation to This second mode of operation. Π····························································································· The detector device is in one of at least two modes of operation. 61009-940112.DOC This paper scale applies to China National Standard (CNS) Α4 specification (210X297 public) 1247894 A8 B8 Patent application scope 申 π patent scope item 17 of the CD player, wherein the indicator during the formation of the disc Pre-embossed on the disc or on the disc. 19. The optical disc player of claim 17, wherein the indicator comprises a pre-embossed groove formed in a land area between the wobble tracks. 2. A disc player as claimed in claim 17, wherein the fingerprint comprises a pre-embossed address index formed in a disc region read by the illumination reading beam. 21. The optical disc player of claim 2, wherein the pre-imprinted address index comprises a spatially addressable pattern of a sub-micron aspect. 22. The optical disc player of claim 17, wherein the indicator comprises a bi-phase mark and a combination of holes carried by the optical disc. 23. A disc player adapted to operate in combination with an output utilization device and a compact disc. The optical disc forms a negative magnetic track having a spiral shape or a Φ on a first surface of the optical disc, the magnetic track being formed to have at least one operation Structure for providing at least a focus error signal generation and a radial tracking error signal generation, the optical disk having an unidentified analyte structure that is confocal positioned with the operational structure forming the information track, The player comprises: a photonic device for generating a laser beam suitable for illuminating the information track and the analyte structure; a disc support device for supporting the optical disc in a rotational relationship with the laser beam; a port such as a compact disc and a far-field laser beam for focusing the rotating beam on the information track and for maintaining the focus on the information track This paper ruler New Zealand® Home Standard (CNS) Α4 specification (2iGx297 public 1 1247894 a focusing device for focusing the servo device; a speed control device including a speed servo control device for controlling the rotational speed of the optical disk in a predetermined manner; for example, when the field beam is irradiated on the rotating information track, Tracking device for radial tracking servo device for radial positioning of field beam; output connector device for forming image signal for connection to external utilization device; sum detector device for combining element and child optical device The sum detector device has at least two modes of operation, including a first mode of operation for generating signals suitable for application to the radial tracking servo and the focus tracking servo, and a second mode of operation for temporarily State interrupts are suitable for application to the radial tracking servo and the focus tracking servo and signals for connecting at least a child read signal to the output connector device; π is used to cross the analysis as the laser beam a device for characterizing the output signal generated by the sum detector when the object is used, such as a laser beam spanning the sub Detecting and displaying the amplitude change of the HF signal during the analysis signal, and measuring the duration of the reflected beam across the analyte signal to thereby provide a substantially qualitative measurement of the size and construction of the analyte; Separating the analyte signal generated by the second mode of operation by the operation signal generated by the first mode of operation, the separating device responding to the operation signal and for switching the detector device from the first mode of operation to The second mode of operation; and the separation device comprises a signal for analyzing the object state to be generated in parallel with the 61090-9401I2.DOC paper size applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm----- ------ 1247894 A8 B8 C8 i________D8 VI. Application for patent range discrimination and separation of tracking signals for signals generated by configurable analytes on the optical disc and for track-reading on the disc A device that produces the smallest change in HF signal during the acquisition period. 24. The optical disc player of claim 23, wherein the detector device comprises a focus error (FE) signal, a radial tracking error signal (TE), and a high frequency read signal (RF). Device. 25. The optical disc player of claim 24, wherein the laser beam is irradiated onto the information track and the laser beam is used to control the laser beam if the track is associated with the substantially static read beam. A device for tangential positioning. 26. A disc player as claimed in claim 24, further comprising a focusing lens of the optical device for use in the proximity of the optical disc if the laser beam is irradiated on the surface having the operation and the analyte is positioned Confocal focusing of the read beam during operation and analysis of the object state on the inverted disc of the side positioning surface. 27. The disc player of claim 24, wherein the tracking servo signal is replied by the tracking servo signal The device reverts to the standard of the CD design from the operational aspect positioned on the disc. 28. The optical disc player of claim 24, wherein the analyte aspect is used to generate a high amplitude, high frequency event of the four column sum (HF, RF) signal to thereby provide correlation of the analyte species Dimensional information. 29. The optical disc player of claim 24, wherein the characterization device further comprises means for identifying a signal generated by the signal generated by the identification of the analyte. 61009-940112.DOC 1247894 30. The optical disc player of claim 2, wherein the laser beam is irradiated on the mm magnetic track and the magnetic track is associated with the substantially static reading beam. A device for controlling the tangential positioning of the laser beam. 31. A disc player adapted to operate in combination with a disc having a spiral or concentric information track formed on a first surface of a disc, the track being formed to have at least one operational structure for providing suitable And at least focusing on the error signal generation and the physical index generated by the tracking error signal, the optical disk having an unidentified analyte structure confocal positioning with the operating structure, the player comprising: an optical device for generating for being suitable for illumination a laser beam is absent on the information track; the optical disk supporting device is configured to support the optical disk in a rotating relationship with the laser beam; if the optical disk is in rotational engagement with the laser beam, the laser beam is focused on the information track And a focus servo for maintaining the focus on the information track; a speed feeding control device for controlling the rotational speed of the optical disc in a predetermined manner; including for controlling when the laser beam is irradiated on the information track The device for tracking the servo device for positioning the laser beam; for controlling the laser beam when it is illuminated on the rotating information track The laser beam positioning tracking servo device; an output connector device for forming an image signal that can be used for connection to an external utilization device; and -10- 61009-940112.DOC. The paper size is applicable to the Chinese National Standard (CNS). A4 size (210X 297 mm). 1247894 A8 B8 ________ A __S___ VI. Patent application: The optical device also includes a detector device having at least two modes of operation. The operation mode includes a first mode of operation for generating a suitable And a signal for applying to the tracking device and the focus tracking servo device, and for generating a signal suitable for application to a signal decoding device for decoding information stored on the information track, the detector device There is a second mode of operation for transient chopping of the signal suitable for application to the tracking servo and the focus tracking servo. 32. The optical disc player of claim 31, wherein the second mode is further configured to connect at least the four columns of sum signals to the output for detecting and characterizing an analyte structure located on the optical disc. 33. A disc player as claimed in claim 3 i or 32, further comprising means for classifying the pattern of the at least one physical characteristic of the analyte structure disposed on the disc or in the disc. 34. A disc player as claimed in claim 3, which also includes means for interpreting the pattern cluster. 35. A disc player as claimed in claim 4, further comprising means for mapping the pattern cluster based on positioning information presented in the digital data. 36. A disc player as claimed in claim 29, further comprising means for tracking the disc and for generating an analyte signal from the analyte structure. 37. A disc player as claimed in claim 36, which also includes means for discriminating the analyte response signal from a normal operational electrical response. 38. The optical disc player of claim 37, which is further comprising: for reading an individual optical disc having at least one readable analyte structure, 掏61009-940112.DOC | j scales S standard A4 specifications _ ^ 297 mm). Ϊ 247894 VI. The device for which the patent application scope is taken. 39. The optical disc player of claim 3, wherein the optical disc is trackable and supports at least one analyte structure that can be read in parallel. 4. A disc player as claimed in claim 39, wherein the device for analyzing comprises means for identifying a pattern of information indicative of the physical characteristics of the analyte structure. The optical disc player of the fourth aspect of the patent application includes a plurality of solid shaped analyte structures that can be read in parallel with the tracking property of the optical disc. The optical disc player of claim 41, which further comprises means for determining the relative physical position of the analyte structure by means of the operational aspect associated with the optical disc. The different analytes comprise a first analyte structure and a second analyte structure. The optical disc player of claim 42 is further comprising a second pattern for reporting the physical properties of the second analyte structure. The apparatus, η determines that the apparatus includes means for reporting the first pattern of the first analyte. &quot; The disc player of claim 42, for example, is for calculating at least the relative physical position of the first and second analyte structures. Α 1 Set the foot of the disc. For example, the disc player of claim 44 of the patent application also includes a skirt for mapping the physical position of the analyte structure on the surface of the individual optical disc. 6. The optical disc player of claim 45, wherein the apparatus for use comprises means for determining at least the first and second analytes I cover a + relative physical position. ° (一 -12- 61009-940112.DOC 巧张尺^^家鲜(CNS) Α4规格(2Κ)] 297 mm). 1247894 A8 B8 47. ΠΓΓ; The 46th _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The object "the individual disc 48. I. Insert, Beixun storage and sample analysis media, including: Zone::::: has a plane surface area and a plurality of separations, elongations, and planes from the plane surface The surface index of the separation; the surface index is sequentially arranged in the substantially parallel information track; the inter-track portion of the surface area of each track separated by the interval of the surface surface area is separated; the outermost information is located at the outermost information a portion of the outer track information track between the tracks on the two sides of the track; the portion between the track and the portion of the track having a common surface area having a width that defines the width of the information track; substantially uniform width from the surface index; a portion of the inter-track between the adjacent information tracks; each of the a-knife-off surface indicators and the spaced portion having a variable length to indicate stored information; and at least one portion of the inter-track portion for identifying the The device in the region of the planar surface region is adapted to support at least one analyte structure. 49. Information storage and sample as in claim 48 The analysis media, its 61009-940112.DOC -13- 1247894 Patent Application Field The information track includes an individual spiral rotation between the inner peripheral portion of the main surface and the peripheral portion of the outer shaft. n The information of the 48th or 49th patent range is recorded and sampled, where the media is dish-shaped. Step 51. π = Scope 48 of the information storage and sample analysis media, the surface indicators include the extension of the main surface of the 52] patent application scope of the 48th information storage and sample analysis media, and the surface indicators include components The elongation of the main surface: = and = information is stored as a separate surface indicator with a negative (four) disc spacing. First 3 · Such as Shen Qing patent scope 4th 士士 advisor 4 in the extension of the m Lu fat, #错存 and sample analysis media 'the depth of the disk 4 1 and the bottom surface of the mark 54: seed two 'face The surface area is separated by a substantially common plane. Bayer storage and sample analysis media, including: Area:::2 * Planar surface area and the plane index separated from the surface of the plane, the extension and separation of the planes of the two regions are sequentially arranged in the surface index a plurality of substantially parallel information magnetic along the surface area of the plane ... adjacent surface index; ^ Shao 7 knife / knife 胄 &lt; each track along the portion of the track surface separated by the inter-track portion 61009-940112.DOC This paper ruler ^ -14- 1247894 as B8 C8 D8 VI. Patent application range parallel information track; the inter-track portion and the spacing portion jointly define the plane surface area; have defined the information track width The separation surface index of substantially uniform width; the inter-track portion between adjacent information tracks having substantially uniform width; each of the separation surface indicators and the spacing portion having a variable length; the separation surface indicator and The spacing portion is used to represent information stored; the separation surface indicator includes an indication of the extension of the major surface; The stored information includes at least one address information for identifying a spatial location on the primary surface; and at least one of the addresses is used to designate a suitable surface location for supporting at least one analyte structure. 55. The information storage and sample analysis medium of claim 5, wherein the separation surface indicator comprises an extension of the major surface of the component and the information is stored for subsequent presentation to a disc having a positive image The separation of the surface indicators. 56. The method of claim 5, wherein the separation surface indicator comprises an extension of the major surface and the information is stored as an indicator of the separation surface immediately following the portion of the disc that has a negative image. 57. For the information storage and sample analysis media of patent application No. 5, 5, 5 or 56, the mark of the extension is a substantially uniform depth. 61009-940112.DOC -15- This paper scale applies to China National Standard (CNS) A4 specification (210X297 mm). 1247894 as Β8 C8 D8 VI. Patent application scope 58. If the patent application scope is 5, 5, 5 or 5 6 information storage and sample analysis media, wherein the information track comprises an individual spiral rotation extending between an inner peripheral portion of the major surface and an outer peripheral portion. 59. For example, the information storage and sample analysis media of Patent Application No. 54, wherein the medium is a dish. 60. An information storage and sample analysis medium, comprising: a main portion having a planar surface region and a plurality of surface indices separating, elongating, and separating from the planar surface region; the separation surface indicators are sequentially arranged in essence In the parallel information track; the adjacent surface index of each track separated by the spaced portion of the planar surface region; the parallel information track separated by the inter-track portion of the planar surface region; The inter-track portion and the spacing portion collectively define the planar surface region; the separation surface index having a substantially uniform width defining the information track width; and the substantially uniform width between the tracks between adjacent information tracks a portion; each of the separation surface indicators and the spacing portion having a variable length; at least a portion of the separation surface indicator and the spacing portion are used to represent storage information; a portion of the planar surface modulation is adapted to support at least one analyte structure; and 61090- 940112.DOC - 16 This paper size is applicable to China Home Standard (CNS) A4 Specification (210X297 mm) _ 1247894 A8 B8 C8 D8, Patent Application Scope = Provide location information to identify the planar surface suitable for supporting the at least-analyte, 'mouth structure' Device. 61 ::: The information storage and sample points of the 48th, 54th or 60th patent range will be included in the interval between the separation surface index and the interval between the laser beam intensity modulations so as to provide The separation is performed by comparing the light between the surface index and the spacing portion and enhancing the recovery of the stored information of the main portion of the child component by light detection. 1 The information storage and sample analysis medium of the 60th item of the Fan Fan Park, wherein the U track comprises an individual spiral rotation extending between the inner peripheral portion of the main surface and the peripheral portion of the outer shaft. 63· 2 Please select the information storage and sample analysis media of the 48th patent range, transfer the surface indicators to the main body of the optical disc material β 64·, apply for the patent scope 48, 54 or 60; the information storage and sample distribution media' Wherein the medium is suitable for conveying surface indicators in the stamping process 4 to the body of the optical disc material. 65. If the information in the 48th, 54th or 6th paragraph of the patent application is in error and the sample analysis media, the media is suitable for use in embossing embossing to transmit surface indicators to the body of the disc material. 66. If the information in the 48th, 54th or 6th paragraph of the patent application is filed and sampled, and the media can be written to the media after the analysis of the analyte structure. 67. For the information storage and sample analysis media of the patent application No. 4, 5, 5 or 6 of which the media is a transmissible CD. 68. If the patent scope of the application is 4, 5, 5 or 6 Information Storage and Samples 61009-940112.DOC -17- 1247894 A8 B8 C8 D8 VI. Scope of Patent Application The media, where the boundary between the separation surface index and the adjacent portion of the track, is substantially linear when viewed in the plane along the direction of the information track. 69. An information storage and sample analysis medium, comprising: a dish-shaped member having a planar surface area on a main portion and a plurality of surface indicators separating, elongating, and separating from the planar surface area; the separation surface The indicator includes an extension mark of the main portion; the separation surface indicator is sequentially disposed in the sequentially parallel information track; the information track includes a main surface of the member extending from the inner peripheral portion to the outer peripheral portion An individual spiral rotation; an adjacent surface index of each track separated by a spaced portion of the planar surface region; the parallel information track is separated by an inter-track portion of the planar surface region; The spacing portion collectively defines the surface area of the surface between the separation surface index and the adjacent portion between the tracks, substantially linear when viewed in the plane along the direction of the information track; the separation surface indicator has Defining the width of the information track width that is substantially uniform; the mark of the elongation is true The uniform depth, the mark is located in a substantially common plane separated from the plane of the planar surface area; the inter-track portion between adjacent information tracks has a substantial 61009-940112.DOC - 18 paper scale applicable to China Standard (CNS) A4 size (210X 297 mm). 1247894 6. Width of application for patent garden; at least one analyte structure; supporting at least one analyte - a portion of the surface is suitable for support for providing The position information is used to identify the device of the applicable structure and the separation surface indicator has a variable length to indicate the stored information, and the portion between the separation surface index and the interval between the child can be incident on the thunder The intensity of the beam is modulated to thereby provide a comparison of the light between the separation surface index and the spacing portion and the recovery of the stored information by the light contrast. 7. In the information storage and sample analysis media of claim 51, the child is marked as forming a pit form opposite to the main part. 71. A compact disc having a readable ear and an analyte structure, the optical disc comprising: an information layer including a trackable structure, wherein the information layer is tracked by a read beam of a disc reader, the traceable structure is Generating a holographic image; and to the y analyte structure, the trackable structure and the analyte structure can be read in parallel by a single optical pickup. &amp; For example, (4) _ 6th item of the disc', wherein the holographic image is projected in a plane that is shared with the structure of the analyte. 73. For example, the disc of the patent scope 7 or 7 is the image of the wobble track. 74------------------------------------------------------------------ The patterned surface facilitates the optical information layer; / 7 - an operational structure coupled to the information layer, the substrate patterned surface being presented when the laser is near an additional surface of the substrate, the operational structure being tracked by the disk drive; The operational structure can read at least one analyte organization in parallel. 75. The optical disc assembly of claim 74, wherein the operating structure is configured to be a front relief image. 76. For example, the optical disc assembly of claim 74, wherein the operating structure can be tracked! ! Positioned in the plane of the information layer, the information layer is a remote surface of the substrate. The optical disk assembly of the patented item 74 is configured to be a negative relief forward image. 7 = Apply for the optical disc assembly of Patent No. 74, wherein the operation can be tracked in the plane of the information layer, and the additional surface of the panel. The signal layer is the optical disc assembly closest to the base = the range of items 74 to 78, wherein the Θ operating structure includes a wobble track. 80. For example, the light of the 74th item of the patent garden, ^ ^ ^ and the operation structure are confocal configurations. The cleavage structure of the knives in the 81 · 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 如 81 81 81 81 81 81 81 81 81 81 The sub-(four) structure 61009-940U2.DOC this (four) ruler 20- 1247894 A8 B8 And the operating structure is a confocal configuration. 83. The optical disc assembly of claim 74, wherein the analyte structure is a characteristic analyte signal element. "Applicable to the optical disc assembly of the 75th, 76th, 77th or 78th patent range, the analyte structure of the AR in the AR is a specific analyte signal element. VIII. 5. For example, the application of the patent scope of the ninth item of Dodge, and inflammation Du-w, shell (the disc component, wherein the analyte structure of the child is a characteristic analyte signal element. wherein the analyte structure is the analyte structure, wherein the analyte structure is wherein the information layer is still packaged. 86. The disc component of the 80 item is a specific analyte signal element. 87. The disc assembly of claim 81 is a specific analyte signal element. The disc assembly of claim 82 is a specific analyte signal element. = the optical disc assembly of claim 8 of the patent application, the splicing layer, the reflective layer, and wherein the substrate patterned surface is present when the laser is near the additional surface of the substrate, the non-analyte structure is laser Arranged close to the reflective layer. 9〇·^ The optical disc assembly of claim 8 wherein the information layer further comprises a reflective layer 'and the non-analyte thereof The optical disk assembly is configured to be readable and disposed on the surface of the optical disk, and the additional surface of the substrate is farther away from the additional surface of the substrate. The optical disk assembly of claim 89 or 9, wherein the non-operational structure is configured On the surface of the reflective layer. 92. - Optical disc assembly with readable non-analyte structure, the optical disc assembly 61009-940I12.DOC -21 - This paper size is suitable for _i^NS) City grid (靡公公酱) Sixth, the application for patents includes: a layer of 2, the information layer has a structure that can be tracked by a CD reader; the second version of 'Auxiliary focus incident beam on the information layer, · and / an analyte structure, the searchable optics The pickup can be read at the same time. The knife is separated, the mouth is made of a single - 93 · For example, the patent scope of the patent is 7th 4th q 9 temple, integrated into the CD...: 9]:::Disc component 'The cover is Non-== the optical element assembly of item 93, the one of which is attached to the optical disk in the 弁 》 》 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 切 切 切 切 切 切 切 切 切 切 切 切 切 切The optical disc assembly of claim 95, wherein the cover is attached to the hinge The disc. 97.==The disc assembly of item 74 or 92, wherein the cover is made of a material group selected from the group consisting of plastic and broken glass 98:: = range "" - wherein the cover Essentially, the cover is essentially 99. The optical disc assembly of claim 198 is composed of polystyrene. 100. The optical disc assembly as claimed in claim 9 is composed of polycarbonate. 101. The optical disc assembly of claim 7 or claim 92, wherein the diameter of the optical axis between 110 and 130 mm in the radial plane has a depth therebetween. </ RTI> </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; a knife 103 having an optical disk with a readable analyte structure, the optical disk comprising: a first reflective layer; a second reflective layer; and an analyte structure disposed on the second reflective layer on a predetermined side A track searchable structure in combination with the first and second reflective layers of the child, the trackable structure being configured to allow tracking of the incident beam of the optical disk drive, and the analyte structure being readablely configurable with the trackable structure. 104. The optical disc of claim 103, wherein the analyte structure and the trackable structure are readable by a single optical pickup. 105. The optical disc of claim 103, wherein the second reflective surface is semi-reflective. 106. The optical disc of claim 105, wherein the analyte structure and the first reflective layer or the surface of the semi-reflective layer are in a confocal configuration. 107. The optical disc of claim 105, wherein the analyte structure and the surface of the semi-reflective layer are in a confocal configuration. 108. The optical disc of claim 1, wherein the analyte structure is confocal with the surface of the first reflective layer. Μ 109. The optical disc of claim 1, wherein the analyte structure is disposed on a laser near the end side of the first reflective layer. 110. If you apply for the CD-ROM of Article 108, the structure of the analyte is 23 61009-940112.DOC. The paper scale applies to the Chinese National Standard (CNS) Α4 specification (210X297 public Chu). 1247894 The first reflective layer is on the distal side of the laser. 1U. The optical disc of claim 10, 106 or 107, wherein the analyte structure is disposed between the first reflective layer and the semi-reflective layer. 112. For example, if you apply for a patent, Fan No. 1 0 3, 1 0 4, 1 〇 5, 1 〇 6 or 1 〇 7), the trackable structure contains a wobble track. 113. For example, the disc of claim 1 of the patent scope, wherein the searchable structure comprises a wobble track. Wherein the track-recoverable structure package, wherein the track-recoverable structure package, wherein the analyte structure and the 114β, such as the optical disk of claim No. 109, contain a wobble track. 115. If the disc of the application for the scope of the invention is in the 110th paragraph, it contains the wobble track. 116. If the optical disc of the scope of claim 1st 1 1 contains a wobble track. 117. For example, the disc of the patent application range 112 has the oscillating track confocal configuration. The ns. The optical disc of claim 105, 106 or 117, wherein the reflective layer and the semi-reflective layer are reversibly separable. II9 - a method for producing an optical disc having an image-operating structure and a embossed at least - an analyte structure readable by the operation structure, the method comprising the steps of: forming an image The orientation and embossing are equal to the standard master 纟 operable stamper; and ° m directly forms the disc substrate from the stamper. 120· A method for fabricating a track-operable structure and a total of 61009-940112.DOC with the operating structure. The paper size is suitable for the SS material (CNS) 24- 1247894 6. The patent application can be fed to / a method of inverting an optical disk to an image and a negative relief before the knife-analyte structure includes the following steps: forming a reverse main film; forming a stamper having an image orientation and a relief opposite to the reverse main sheet; and directly The stamper forms the disc substrate. The method of claim 119 or 12, wherein the formation of the optical disc is performed in a diversified digital optical disc (DVD) mold. 122. A method for making a track-travelable optical disc having a readable analyte structure, the method comprising the steps of: applying a reflective layer to an inversion prepared by the method of claim 119 or 12 The disc substrate defines a surface; and the analyte structure is confocal with the reflective layer. 123. The method of claim 122, wherein the configuring step further comprises configuring the analyte structure to be a laser approaching the reflective layer. 124. A method for making a track-provisionable optical disc having a readable analyte structure, the method comprising the steps of: applying a reflective layer to each of items 74 to 78 of the patent application scope The patterned surface of the optical disc substrate; and a confocal configuration of the analyte structure with the traceable operational structure. 125. The method of claim 124, wherein the configuring step further comprises configuring the analyte structure to be a laser approaching the trackable operational structure. 126. The method of claim 1, wherein the trackable structure comprises a wobble track. -25 61009-940112.DOC This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 public).