TW201941832A - Liquid separation device and use method thereof including a tubular container and a piston - Google Patents

Abstract

The present invention provides a liquid separation device including a tubular container and a piston, wherein an accommodating space is provided in the tubular container for storing liquid, and then it is placed in a centrifugal machine for liquid separation; the front end of the tubular container is tapered to form a cone, a cylinder protrudes and extends from the front end of the cone, the interior of the cylinder is hollow to form a guide channel, the front end of the guide channel passes through the cylinder to form a tubular opening, the front end of the accommodating space extends into the interior of the cone and is connected with the guide channel, and the rear end of the accommodating space passes through the tubular container to form an opening; and through the opening the piston is disposed and moves in the tubular container, the piston has a soft and elastic structure so that when being pressed it expands and compresses the tubular container, and the rear end of the piston is provided with a fixing column so that pressing the fixing column is facilitated to control the movement of the piston.

Description

Liquid separation device and using method thereof

The invention belongs to a liquid separation device, in particular to a separation device used for separating blood components, increasing the efficiency of liquid separation, improving the collection quality of the components in the liquid, and effectively preventing pollution.

Human blood mainly contains various components such as plasma, red blood cells, white blood cells, and platelets, each of which has a different purpose to maintain the normal functioning of the human body and enable a healthy life for the human body.

Under normal conditions, blood rich in multiple components can completely provide the human body, but in medical applications, sometimes it is necessary to focus on a single component in the blood. For example, platelets can secrete a variety of growth hormones and have a good auxiliary effect on tissue regeneration such as articular cartilage. Therefore, it is necessary to perform blood separation operation to collect the required components.

A common blood separation technique is to put blood into a blood collection tube and use a centrifuge to rotate the blood collection tube. Under the effect of centrifugal force, the main components of blood are concentrated in the upper and lower layers of the blood collection tube; The upper layer of liquid is light yellow plasma, the lower layer of liquid is dark red blood cells, and the middle layer of the upper and lower layers is light gray with high concentration of platelet plasma (PRP).

Because PRP has medical and commercial value, how to improve the efficiency and quality of PRP collection has become a very important issue in the industry.

Conventional technologies, such as the "blood separation device" of the Republic of China Patent No. M483797, provide a blood separation device with a simplified structure, which is used to improve the convenience of operation and increase the accuracy of blood separation, while reducing blood during operation Risk of pollution.

However, the sleeve (30) of the conventional technique is a general cylindrical structure, which does not assist in collecting PRP itself, and the conventional technique uses a push rod (11) to push the groove body (13) to move To obtain the required PRP, the accuracy of this operation is limited, and the obtained PRP concentration is difficult to maintain.

Another conventional technology, such as the "centrifuge tube structure" of the Republic of China Patent No. I490493, also provides a blood separation device with a simplified structure and increased ease of operation. The conventional structure includes a neck constriction (21) The tube body (2), when operating, a push rod (42) is used to push the piston portion (41) to concentrate the PRP on the necked portion (21), and then the PRP is extracted with a thin needle (6).

However, the necked portion (21) of the tube body (2) is obviously fragile, and it is easy to rupture during operation, which is dangerous; secondly, the narrow necked portion (21) will inevitably hinder the separation of blood when the centrifuge is rotated. , Resulting in increased operating time and lowered quality; furthermore, the action of extracting PRP through the upper liquid with the fine needle (6) may also cause the PRP to mix with the upper liquid to reduce the quality.

It can be seen that there is still room for improvement in conventional technology, and it is urgent to be overcome by the industry.

In view of the various problems generated by the conventional technology, the inventor of this case has been eager to improve and innovate. After years of painstaking research, he finally successfully developed this liquid separation device.

The main purpose of the present invention is to provide a liquid separation device that can effectively separate liquids, concentrate and increase the concentration of layered liquids, facilitate the collection of required liquids, and improve the quality of collected liquids.

Another object of the present invention is to provide a liquid separation device, which has a tightly sealed structure and does not touch the outside during operation, which can prevent pollution and prevent infection.

Another object of the present invention is to provide a liquid separation device, which can accelerate the progress of the separation process and maintain the freshness of the separated liquid to exert the effect of separating the liquid.

A liquid separation device provided by the present invention mainly includes a tubular container and a piston.

The inside of the tubular container has a containing space for storing liquid to perform the centrifugal force rotation operation of the centrifuge; the front end of the tubular container is tapered to form a cone, and the front end of the cone is protruded with a cylinder, and a cylindrical body is formed inside the cylinder. The front end of the approach path passes through the column to form a pipe opening; the rear end of the accommodation space passes through the tubular container to form an opening, and the front end of the accommodation space extends to the inside of the cone and connects with the approach path. , The tube opening at the front end of the tubular container is completely connected with the opening at the rear end; the piston is moved into the tubular container through the opening, and the piston is a soft elastic structure, which will expand under pressure to force the tubular container. In order to maintain the air-tightness in the accommodating space, a fixed post is provided at the rear end of the piston to facilitate pressing the fixed post to control the movement of the piston.

With the foregoing structure, blood is first placed in the containing space in the tubular container when in use, and then the tubular container is placed in the centrifuge to begin the centrifugation operation; It is separated into three layers by centrifugal force, the upper layer is light yellow plasma, the lower layer is dark red blood cells, and the middle layer of the upper and lower layers is light gray PRP; then a syringe is connected at the front of the tubular container, and then After pulling the inner rod of the syringe, the upper layer of the plasma is taken out, and finally another syringe is connected to the front end of the tubular container, and then the fixed column is forced to push the PRP into the syringe to complete the collection of the PRP.

In the present invention, due to the setting of the cone, when the PRP is to be led out of the tubular container for collection, the PRP will aggregate in the accommodation space at the cone, so the concentration of the PRP is greatly increased, thereby improving the Collection rate of PRP; a cone angle is formed between the rear end surface of the cone and the surrounding wall surface of the cone, and the cone angle is inclined from 20 degrees to 85 degrees.

The cylinder can be tightly combined with the front end of the injection cylinder of the public specification, and the outer surface of the cylinder can be provided with a thread for connecting with the injection cylinder of the public specification, so that the front end of the injection cylinder penetrates into the lead. It reaches the connection end of the cone and the column to increase the concentration of PRP and avoid pollution. The front end of the column can also be connected with a three-way valve for connection operation.

Similarly, the nozzle cap is provided with threads similarly to the bottom cap, and is connected with the threads on the outer surface of the column and the threads on the outer surface of the tubular container to increase the tightness when the tubular container is closed.

In addition, in the present invention, a push rod is put in the middle of the bottom cover, and the front end of the push rod is connected to the fixed post to push the fixed post and the piston to move; the push rod and the fixed post are connected in a threaded manner. The push rod is tightly combined with the fixing post by a thread, so that the piston can be stably moved.

A perforation is provided in the middle of the bottom cover, and the push rod passes through the perforation to be connected to the fixing column. The position of the perforation is used to provide support for the push rod, and further, the push rod and the bottom cover Threads are provided between the perforations, so that the push rod can slowly rotate the piston by using the thread, so that the push rod can move the piston more stably and accurately, and improve the quality of the PRP collection.

The push rod is rotated manually or electrically to move the piston.

Furthermore, the piston is a soft elastic structure such as rubber, which expands when pressed, and compresses the tubular container, and a buffer space is provided between the piston and the fixed post, so that the piston is easier to expand and deform when subjected to pressure. The airtightness in the accommodation space is maintained.

In addition, the surface of the tubular container, cone, and cylinder is provided with a scale, which can immediately observe or calculate the collected capacity when the PRP is concentrated, and use the pusher to control the stop position of the piston to obtain different high-concentration platelet plasma. Including white blood cells-high concentration platelet plasma, low white blood cells-high concentration platelet plasma.

In addition, a convex lens is provided on the cylinder, and the convex lens can enlarge the connection ends between the components of the blood to improve the collection quality of PRP.

5-1 ~ 5-5‧‧‧step

100‧‧‧Liquid separation device

1‧‧‧ tubular container

11‧‧‧ accommodation space

12‧‧‧ cone

13‧‧‧ cylinder

131‧‧‧Thread

14‧‧‧ Approach

15‧‧‧ nozzle

16‧‧‧ opening

161‧‧‧Thread

17‧‧‧ scale

18‧‧‧ convex lens

2‧‧‧ Pistons

21‧‧‧ buffer space

3‧‧‧ nozzle cover

31‧‧‧Thread

4‧‧‧ bottom cover

41‧‧‧Thread

42‧‧‧Thread

5‧‧‧ fixed post

51‧‧‧Thread

6‧‧‧Put

61‧‧‧Thread

7‧‧‧ blood

71‧‧‧ Plasma

72‧‧‧ red blood cells

73‧‧‧PRP

8, 8'‧‧‧ syringe

81‧‧‧ inner pole

θ‧‧‧ cone angle

62‧‧‧Thread

FIG. 1 is an exploded perspective view of the liquid separation device of the present invention; FIG. 2 is a plan exploded view of the liquid separation device; FIG. 3 is a plan exploded view of the liquid separation device; and FIG. A sectional view of the structure; FIG. 5 is an operation flowchart of the liquid separation device; FIGS. 6A to 6D are diagrams of structural changes of the liquid separation device during operation; and FIG. 7 is a schematic structural diagram of the liquid separation device provided with a convex lens.

In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. The specific embodiments described here are only used to explain the present invention, and are not intended to limit the present invention.

The invention provides a liquid separation device, which is mainly used for blood separation to collect PRP for various applications. Of course, it is not limited to the separation of blood. Any device that can be used for the separation of liquid components by a centrifuge belongs to the protection scope of the present invention.

Please refer to FIG. 1 to FIG. 4. The liquid separation device 100 according to the present invention mainly includes a tubular container 1 and a piston 2.

The tubular container 1 has an accommodation space 11 therein for storing liquid for separating components of the centrifuge; the front end of the tubular container 1 is tapered to form a cone 12, and the front end of the cone 12 is protruded with a cylinder 13. An introduction channel 14 is formed inside the column 13, and the front end of the introduction channel 14 passes through the column 13 to form a nozzle 15; the rear end of the accommodation space 11 passes through the tubular container 1 to form an opening 16, and the front end of the accommodation space 11 It extends to the inside of the cone 12 and is connected to the guide channel 14 so that the nozzle 15 at the front end of the tubular container 1 is completely connected with the opening 16 at the rear end; in actual use, the column 13 The nozzle 15 may be covered with a nozzle cover 3, and the opening 16 of the tubular container 1 is also covered with a bottom cover 4 to seal the tubular container 1 for centrifugal operation; and the piston 2 is inserted into the opening 16 The tubular container 1 moves, and the piston 2 is a soft elastic structure, which expands under pressure to force the tubular container 1 to maintain the gas in the accommodation space 11. Tightness, a fixed post 5 is provided at the rear end of the piston 2 to facilitate pressing a push rod 6 on the fixed post 5 to control the movement of the piston 2.

Please refer to FIG. 5 to FIG. 6D. The steps of applying the aforementioned structure to the separation of blood 7 to collect PRP 73 are briefly described as follows: 5-1. First, the blood 7 is placed in the accommodation space 11 in the tubular container 1 ( Fig. 6A), then the nozzle cover 3 and the bottom cover 4 are locked tightly, so that the containing space 11 becomes a closed structure; 5-2. The tubular container 1 is placed in the centrifuge, and the centrifugation operation is started. The centrifugal force is about 100g ~ 4000g, and the time is about 5-20 minutes; 5-3. At this time, the blood 7 is separated into three layers, the upper layer is light yellow plasma 71, the lower layer is dark red blood cells 72, and the upper and lower layers The middle layer of the junction is light gray PRP 73 (Fig. 6B); 5-4. The front end of the tubular container 1 is connected to an injection cylinder 8, and then the inner rod 81 of the injection cylinder 8 is pulled back, resulting in the production of the inside of the injection cylinder 8. Negative pressure, so the plasma 71 on the upper layer of the containing space 11 is attracted to move into the syringe 8, and the plasma 71 on the upper layer is taken out (FIG. 6C); and 5-5. The syringe 8 is removed and placed in the tube The front end of the container 1 is connected with another injection cylinder 8 ′, and then the push rod 6 is inserted into the fixed column 5, and the push rod 6 is forced to push the fixed column 5 so that the piston 2 is at the same position. Forward-shaped container 1, and thus the PRP 73 is pushed into the syringe barrel 8 ', the collected PRP 73 is completed (FIG. 6D).

It is known from the foregoing structure and steps that the present invention also has the syringe 8 and 8 'for extracting 7 components of blood or the piston 2 for extruding 7 components of blood, which can be flexibly dispatched as required to obtain more conveniently and accurately. Desired PRP 73.

Please refer to FIG. 1 and FIG. 2 again. An important feature of the present invention is the arrangement of the cone 12. When the PRP 73 is to be led out of the tubular container 1 for collection, the tubular container 1 The front end of 1 is gradually reduced, and the PRP 73 will aggregate in the accommodating space 11 at the cone 12, so the concentration of the PRP 73 is greatly increased, thereby increasing the collection rate of the PRP 73.

A cone angle θ is formed between the rear end surface of the cone 12 and the surrounding wall surface of the cone. In order to obtain a better cone angle θ, the inventor separately conducted experiments based on different cone angles θ to obtain the The collection rate of PRP 73 is as follows:

It can be known from the above table that the collection rate of the PRP 73 will increase as the cone angle θ of the cone 12 increases. The cone angle θ of the present invention is preferably set to 25 degrees to 85 degrees.

Please refer to FIG. 6C and FIG. 6D again. The cylinder 13 of the present invention can also facilitate the collection of the PRP 73. The cylinder 13 can be tightly combined with the front end of the syringe 8 in accordance with the regulations and make the front end of the syringe 8 deep. The approach path 14 reaches the connection end of the cone 12 and the pillar 13 to increase collection Concentration at PRP 73 and to avoid contamination, a three-way valve (not shown) can also be connected to the front end of the column 13 for connection work.

Similarly, as shown in FIG. 1 to FIG. 3 and FIG. 6C to FIG. 6D, the outer surface of the cylinder 13 may be provided with a thread 131 for connecting with the injection cylinder 8 of a public standard, and then the thread 131 is used. Increase the tightness of the connection.

Referring again to FIGS. 1 to 3, the nozzle cover 3 is provided with threads 31 and 41 similarly to the bottom cover 4, so as to face the thread 131 of the outer surface of the cylinder 13 and the opening 16 of the tubular container 1. The threads 161 on the surface are connected to increase the airtightness of the tubular container 1 when it is closed.

Please refer to FIG. 3, FIG. 4, and FIG. 6D. The front end of the push rod 6 is connected to the fixed column 5 to push the fixed column 5 and the piston 2 to move. This causes jitter, which affects the quality of collecting the PRP 73. Therefore, the push rod 6 and the fixed post 5 are connected by threads 51 and 61. When the push rod 6 and the fixed post 5 are tightly coupled by threads 51 and 61, , You can steadily push the piston 2 to move and improve the quality of the PRP 73 collection.

As shown in FIG. 4, the push rod 6 has a slender structure. Even if it is connected by a thread 61, it can still affect the collection of the PRP 73 due to left and right swing. Therefore, the present invention has a perforation in the middle of the bottom cover 4. Let the push rod 6 pass through the perforation to connect with the fixing post 5 and use the perforation position to provide support for the push rod 6. The push rod 6 can directly push the fixed post 5 without relying on the threads 51, 61, so that The pusher 6 is more stable when pushed, improving the quality of the PRP 73 collection.

Alternatively, threads 62 and 42 are further provided between the push rod 6 and the perforation of the bottom cover 4 to make the push rod 6 slowly rotate the piston 2 by using the threads 62 and 42 to make the push rod 6 more stable. Move the piston 2 accurately to improve the quality of the PRP 73 collection.

In addition, please refer to FIG. 3 and FIG. 4, the piston 2 is a soft elastic structure, for example For example, rubber and silicon rubber will expand when pressed to force the tubular container 1; and the present invention further provides a buffer space 21 between the piston 2 and the fixed column 5 to make it easier for the piston 2 to expand when under pressure. Deformation maintains the airtightness in the accommodation space 11.

Furthermore, as shown in FIG. 1, in order to facilitate the calculation of the collected PRP 73 component capacity, the surface of the tubular container 1, the cone 12, and the column 13 is provided with a scale 17, and the collected capacity can be calculated immediately when the PRP 73 is concentrated; By using the scale 17 on the surface of the liquid separation device 100 and controlling the stop position of the piston 2 with the pusher 6, different high-concentration platelet plasmas can be obtained, including white blood cells-high-concentration platelet plasma, low-leukocyte-high-concentration platelet plasma.

Or as shown in FIG. 7, a convex lens 18 is provided on the cylinder 13, and the convex lens 18 can enlarge the connection ends between the components of the blood 7 to improve the collection quality of the PRP 73.

By using the aforementioned liquid separation device 100 of the present invention, it is indeed possible to increase the convenience of collecting the PRP 73 and increase the efficiency of work, and greatly increase the quality of the PRP 73 collection, so that the use of the PRP 73 can be more in line with actual operation. Demand, while reducing the possibility of pollution, can also further improve safety.

The above are only preferred embodiments of the present invention, and are not intended to limit the scope of implementation of the present invention. Without departing from the spirit and scope of the present invention, modifications or equivalent replacements of the present invention should all be covered by the scope of patent application of the present invention. Within the scope of protection.

Claims (10)

A liquid separation device is characterized in that: a tubular container, the front end of which is tapered to form a cone, the front end of the cone protrudes from a cylinder, the tubular container has an accommodation space inside, and the rear end of the accommodation space penetrates the tubular container An opening is formed, and the front end of the accommodating space extends to the inside of the cone and penetrates the interior of the column to form an approach channel. The front end of the approach channel penetrates the column to form a nozzle; a piston is inserted through the opening. Moving in the tubular container, the piston is a soft elastic structure, and the rear end is provided with a fixed column; a bottom cover covering the open end of the column; a nozzle cover covering the pipe opening of the column end. The liquid separation device according to claim 1, wherein the surface of the tubular container, the cone, and the cylinder is provided with a scale. The liquid separation device according to claim 1, wherein a cone angle is formed between a rear end surface of the cone and a peripheral wall surface of the cone, and the cone angle is inclined by 20 to 85 degrees. The liquid separation device according to claim 1, wherein the outer surfaces of the cylinder and the tubular container are provided with threads. The liquid separation device according to claim 1, wherein the cylinder is provided with a convex lens. The liquid separation device according to claim 1, wherein a push rod is inserted in the middle of the bottom cover, and the front end of the push rod is connected to the fixed post to push the fixed post and the piston to move. The liquid separation device according to claim 6, wherein the push rod and the fixing post are connected in a threaded manner. The liquid separating device according to claim 6, wherein the push rod directly pushes the piston, and a thread is provided between the push rod and the bottom cover, so that the push rod uses the screw to rotate slowly and stably and accurately. The piston. The liquid separation device according to claim 1, wherein a buffer space is provided between the piston and the fixed column, so that the piston is easily expanded and deformed under pressure. A method for using a liquid separation device, which is characterized by: a. Placing blood into a liquid separation device and covering it to seal the liquid separation device; b. Placing the liquid separation device in a centrifuge for centrifugation; c. The blood is separated into two distinct layers, the upper layer is light yellow plasma, and the lower layer is dark red blood cells, and a thin layer of light gray misty area is formed at the junction of the upper and lower layers, and the light gray misty The area is high-concentration platelet plasma (PRP, Platelet Rich Plasma); d. Remove the plasma on the upper layer of the liquid separation device by connecting the nozzle at the front end of the liquid separation device to the injection cylinder, and the inner rod of the injection cylinder Pull back to cause negative pressure in the syringe to attract the upper plasma layer in the liquid separation device to flow into the syringe; e. Take out the high-concentration platelet plasma by removing the The nozzle is connected to the injection cylinder of 5 ml, and a push rod is placed at the rear end of the liquid separation device. The push rod rotates in a threaded manner, and pushes the piston from the back to the front to slide toward the nozzle end to collect the upper and lower two. The light gray misty zone at the layer boundary; f. Using the scale on the surface of the liquid separation device and controlling the stop position of the piston with the pusher, different high-concentration platelet plasmas can be obtained, including white blood cells-high-concentration platelet plasma, Low white blood cells-high concentration of platelet plasma.