TW201932133A - Extracting method of cordyceps cicadae extract and the purpose thereof - Google Patents

Extracting method of cordyceps cicadae extract and the purpose thereof Download PDF

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TW201932133A
TW201932133A TW107103098A TW107103098A TW201932133A TW 201932133 A TW201932133 A TW 201932133A TW 107103098 A TW107103098 A TW 107103098A TW 107103098 A TW107103098 A TW 107103098A TW 201932133 A TW201932133 A TW 201932133A
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extraction
filtrate
microwave
solvent
extract
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魏志豪
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明谷農業生技股份有限公司
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Abstract

The invention provides extracting method of cordyceps cicadae extract and the purpose thereof, which uses fresh entity of the cordyceps cicada's seed to be frozen via wall-breaking effect in the temperature difference way so that the cell wall of the cordyceps cicadae can be broken and the active ingredients can be extracted easily. The frozen cordyceps cicada's seed is grinding physically and microwave extracted to obtain extraction thereof by controlling the temperature below 40 DEG C to vacuum decompression, concentrate, evaporate and filtrate. Accordingly, the rate of transformation of the marker constituents such as cordycepin, adenosine, HEA and SOD enzyme etc. can be lowered via rapid low-temperature microwave extracted and increase the activity of the extraction and achieve the effect of not destroy the ingredients and high extractability. Further, the extraction of the cordyceps cicadae can be applied to skin care products and comprises the effects of anti-bacterial, anti-inflammation and repair.

Description

蟬花萃取液之萃取方法及其用途Extraction method of sassafras extract and its use

本發明係有關於一種蟬花萃取液之萃取方法及其用途,尤指一種於萃取過程可保持蟬花活性成分,並降低指標成分質變機率的萃取方法為其應用發明者。The invention relates to an extraction method of a sassafras extract and a use thereof, in particular to an extraction method capable of maintaining the active ingredient of the scented flower during the extraction process and reducing the probability of qualitative change of the index component.

按,近年來由於冬蟲夏草價格不斷攀升,科學家積極的尋找替代用的蟲草;在老藥新用的潮流下發現了使用最久遠,記載最古老的蟲草『蟬花』。金蟬花,也稱蟬花,是中國的傳統名貴中藥材,為寄生在蟬上的一種蟲草真菌;由於蟬花性屬偏涼,對於地處亞熱帶容易有濕熱體質的民眾來說可以平衡體內循環,比起溫熱的冬蟲夏草及蛹蟲草而言有著更適合體質的調性,被稱為『南方蟲草之后』!According to the recent increase in the price of Cordyceps sinensis, scientists are actively looking for alternative Cordyceps; under the trend of new drugs, the oldest used Cordyceps sinensis has been found. Calendula, also known as sassafras, is a traditional Chinese herbal medicine in China. It is a cordyceps fungus that is parasitic on the scorpion. Because it is cold, it can balance the body for people who are prone to heat and heat in the subtropical zone. The cycle is more suitable for physical fitness than the warm Cordyceps and Cordyceps, and is called "after the southern Cordyceps"!

自古以來蟬花便為藥食兩用的蟲生真菌一直應用於強身健體,防病療疾,早在公元五世紀的南北朝便已經有藥用的記載,為記載最早,使用最久的蟲草類真菌。蟬花作為傳統藥食兩用的中藥材,不僅沒有有毒的記載,而且是唯一記載可以給兒童甚至嬰兒服用的中藥材,常用於小兒感冒、夜啼、發燒等症狀,嬰幼兒可食,具有優良藥效與極佳的安全性。Since ancient times, the worm-like fungus, which has been used for both medicine and food, has been used for physical fitness and disease prevention. It has been recorded in the Southern and Northern Dynasties since the fifth century AD. It is the earliest and longest used Cordyceps. Fungi. As a traditional Chinese medicine for traditional medicine and food, thistle is not only toxic, but also the only Chinese herbal medicine that can be used for children and even babies. It is often used in children with colds, nightingales, fever, etc. Excellent efficacy and excellent safety.

蟬花在醫療與保健上有很中重要的生理活性物質,包括腺苷、蟲草素、蟲草酸、氨基酸、維生素B&E、微量元素、蟲草多醣、SOD(超氧化物歧化酶)。其中最重要的指標成分有以下:Alfalfa has important physiologically active substances in medical and health care, including adenosine, cordycepin, cordycepic acid, amino acids, vitamin B&E, trace elements, cordyceps polysaccharide, SOD (superoxide dismutase). The most important indicators are the following:

1、線苷可擴張血管,降低血壓,減慢心率及抗血小板凝結,有抗發炎、抗輻射,抗腫瘤等功效,為金蟬花主要指標成分,含量高於冬蟲夏草四倍。1, glucoside can dilate blood vessels, lower blood pressure, slow heart rate and anti-platelet aggregation, anti-inflammatory, anti-radiation, anti-tumor and other effects, is the main indicator component of calendula, the content is four times higher than Cordyceps sinensis.

2、蟲草素為具抗菌活性的核苷類,能抑制癌細胞的生長,有抑菌、抗腫瘤、抑制病毒、降低血清膽固醇等功用。此外臨床作用也證實蟲草素能補精填髓、強身健體,對於高血壓血酯的預防與緩解有顯著療效。2. Cordycepin is a nucleoside with antibacterial activity, which can inhibit the growth of cancer cells, and has the functions of inhibiting bacteria, resisting tumors, inhibiting viruses, and lowering serum cholesterol. In addition, the clinical effect also confirmed that cordycepin can replenish and replenish the marrow, strengthen the body, and has a significant effect on the prevention and remission of hypertensive blood esters.

3、蟲草酸又稱甘露醇,可以促進新陳代謝並顯著地降低血壓,還有鎮靜止咳,擴張氣管等功效。蟬花具有高含量的甘露醇,勝過野生冬蟲夏草。3, Cordycepic acid, also known as mannitol, can promote metabolism and significantly lower blood pressure, as well as calming cough, dilating the trachea and other effects. Thistle has a high content of mannitol, which is better than wild Cordyceps.

4、蟲草多糖為一種高支鏈的半乳甘聚醣。能促進淋巴細胞轉化,提高免疫力,起固本扶正作用,同時能提高肝臟解毒能力,起到護肝的作用。4. Cordyceps polysaccharide is a highly branched galactomannan. It can promote lymphocyte transformation, improve immunity, strengthen the function of strengthening the body, and at the same time improve the liver's ability to detoxify and protect the liver.

5、SOD(超氧化物歧化酶)可消除體內自由基,為人體第一道抗氧化防線,具有抗衰老抗癌等作用。5, SOD (superoxide dismutase) can eliminate free radicals in the body, is the body's first antioxidant defense line, with anti-aging and anti-cancer effects.

6、硒又稱為生命元素。硒的作用是合成硒蛋白和硒酵素。硒酵素的主要功能是抗氧化與調節甲狀腺素的作用,可以代謝脂肪酸過氧化物,減少有害自由基的產生,保護細胞膜與基因,也保護細胞重要的蛋白質。硒缺乏會改變免疫功能,使人易受病毒的感染,及增加癌症的危險。6, selenium is also known as the life element. The role of selenium is to synthesize selenoproteins and selenium enzymes. The main function of selenase is anti-oxidation and regulation of thyroxine. It can metabolize fatty acid peroxides, reduce the production of harmful free radicals, protect cell membranes and genes, and protect important proteins of cells. Lack of selenium can alter immune function, make people susceptible to viral infections, and increase the risk of cancer.

7、HEA為蟬花特有的腺苷衍生物,為極強的非成癮鎮痛消炎劑,可取代嗎啡作為麻醉使用。7. HEA is a specific adenosine derivative of scutellaria, which is a strong non-addictive analgesic and anti-inflammatory agent, which can replace morphine as an anesthetic.

8、ISP-1為蟬花特有的多球殼菌素,可雙向調節免疫,改善自體免疫疾病。8, ISP-1 is a unique globulin, which can regulate immunity and improve autoimmune diseases.

由上述指標成分可知[經現代藥理學研究證明],蟬花具有諸多功能:提高免疫力、改善肝腎功能、調節酯類代謝、降血酯、瘦身、抗癌、促進造血、降血壓、降血糖、解熱鎮痛、鎮靜安眠、抗疲勞、抗衰老、美白美顏等。蟬花具有廣泛的藥理作用,可以說本身就是一個複雜的天然藥方,體現了中藥補益的本質,實為一極優良的藥食兼用真菌。According to the above-mentioned index components, [through modern pharmacological research], thistle has many functions: improving immunity, improving liver and kidney function, regulating ester metabolism, lowering blood ester, slimming, anti-cancer, promoting hematopoiesis, lowering blood pressure, lowering blood sugar. , antipyretic and analgesic, calm and sleep, anti-fatigue, anti-aging, whitening and beauty. Thistle flower has a wide range of pharmacological effects. It can be said that it is a complex natural prescription, which reflects the essence of traditional Chinese medicine tonic, and it is an excellent medicine and food fungus.

現代營養學研究顯示蟬花營養物質豐富,富含蛋白質,氨基酸,脂肪酸,多種維生素及微量元素;在現代藥理學研究中發現除了具有其他蟲草所缺乏的解熱鎮痛、鎮定安眠效果外,同時具有極為強大的護肝救腎功能。與野生冬蟲夏草相比,蟬花的營養成分與冬蟲夏草相似,甚至在總體營養成分上,人工培育的蟬花勝過野生冬蟲夏草。因此,人工培育的蟬花可作為冬蟲夏草優良的替代品。Modern nutrition research shows that scented flowers are rich in nutrients, rich in protein, amino acids, fatty acids, multivitamins and trace elements. In modern pharmacological research, in addition to the antipyretic analgesic and calming effects of other Cordyceps, it is extremely Strong liver and kidney function. Compared with wild Cordyceps sinensis, the nutrient composition of thistle flower is similar to that of Cordyceps sinensis. Even in the overall nutrient composition, the artificially cultivated silk flower is better than the wild Cordyceps sinensis. Therefore, artificially cultivated silk flowers can be used as an excellent substitute for Cordyceps sinensis.

綜上資訊可知,蟬花具有豐富營養物質,於業界研究開發各種萃取方式來提煉出萃取液;一般採子實體材料進行熱風烘乾,以溫度60度c,烘乾時間24小時,再將其研磨成粉體,再以 熱水回流法(溫度高、活性低),或酒精回流法(4小時、時間長、溫度高),或超聲波法(40分鐘、時間長、溫度高)等方式萃取,而上述諸等方式具有時間長、溫度高、破壞成份及萃取率低的缺失,導致市售商品價格高昂且效果不佳的問題。In summary, the flower is rich in nutrients, and various extraction methods are researched and developed in the industry to extract the extract; generally, the raw material is used for hot air drying at a temperature of 60 ° C, drying time for 24 hours, and then Grind into powder, and then extract by hot water reflux method (high temperature, low activity), alcohol reflux method (4 hours, long time, high temperature), or ultrasonic method (40 minutes, long time, high temperature) However, the above-mentioned methods have the problems of long time, high temperature, destructive components, and low extraction rate, resulting in high price and poor effect of commercial products.

今,發明人秉持多年該相關行業之豐富設計開發及實際製作經驗,針對人工培育的蟬花進行萃取技術的開發研究,提供一種蟬花萃取液之萃取方法及其用途,以期達到保留並萃取出最佳指標成分、營養成分的技術,使其具有更佳實用價值性之目的者。Today, the inventor has years of experience in the design, development and practical production of this related industry, and has developed an extraction technique for artificially cultivated silk flowers, providing an extraction method and application of the silk flower extract to achieve retention and extraction. The technology of the best indicator ingredients and nutrients makes it more useful for practical purposes.

本發明之主要目的在於提供一種蟬花萃取液之萃取方法及其用途,尤其是指一種於萃取過程可保持蟬花活性成分,並降低指標成分質變機率的萃取方法與應用為其目的。The main object of the present invention is to provide an extraction method and application of the scented flower extract, in particular to an extraction method and application for maintaining the active ingredient of the scented flower during the extraction process and reducing the probability of qualitative change of the indicator component.

本發明蟬花萃取液之萃取方法主要目的與功效,係由以下具體技術手段所達成:The main purpose and efficacy of the extraction method of the scented flower extract of the present invention are achieved by the following specific technical means:

其主要採取生鮮的蟬花子實體經由冷凍破壁方式,使其在冷凍後可以使得蟬花細胞壁破裂,讓活性成分更容易萃出;其主要於蟬花子實體冷凍後,再進行物理粉碎與多次微波萃取以取得濾液,並將溫度控制低於40度C進行真空減壓濃縮蒸發並過濾雜質而獲得萃取液;藉此,利用低溫微波萃取速度快,可以降低蟲草素、腺苷、HEA及SOD酵素等指標成分質變機率,並增加萃取液活性,達到不破壞成分且萃取率高的功效者。It mainly adopts the fresh scutellaria fruit body through the freezing and breaking method, so that after freezing, the cell wall of the scorpion flower can be broken, so that the active ingredient can be more easily extracted; it is mainly after the freezing of the scorpion fruit body, and then physically pulverized and repeatedly Microwave extraction to obtain the filtrate, and the temperature control is lower than 40 ° C, vacuum decompression concentrated evaporation and filtration of impurities to obtain an extract; thereby, the use of low temperature microwave extraction speed can reduce cordycepin, adenosine, HEA and SOD Indicators such as enzymes have a qualitative change probability and increase the activity of the extract to achieve the effect of not destroying the components and having a high extraction rate.

本發明蟬花萃取液之萃取方法的較佳實施例,其步驟為a)冷凍蟬花子實體;b)物理粉碎;c)一次微波萃取;d)二次微波萃取;e)三次微波萃取;f)濾紙過濾;g)孔隙過濾。A preferred embodiment of the method for extracting the scented flower extract of the present invention comprises the steps of: a) freezing the scorpion fruit body; b) physical pulverization; c) one microwave extraction; d) secondary microwave extraction; e) three microwave extraction; Filter paper filtration; g) pore filtration.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟a)中係採用生鮮的蟬花子實體於低溫零下10-30度c,冷凍時間8-24小時進行冷凍蟬花子實體,其中以低溫零下30度c,冷凍時間12小時為最佳。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein in step a), the fresh scorpion fruit body is frozen at a low temperature of 10-30 degrees C and the freezing time is 8-24 hours, wherein The low temperature is minus 30 degrees c, and the freezing time is 12 hours.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟b)中係將冷凍後之蟬花子實體加入適量溶劑並經由高速物理粉碎機進行粉碎處理,而得粉碎液。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein in step b), the frozen scorpion fruit body is added to an appropriate amount of solvent and pulverized by a high-speed physical pulverizer to obtain a pulverized liquid.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟c)中係將含有蟬花子實體碎渣的粉碎液進行第一次微波加熱萃取,可採用微波設備進行加熱2分鐘,於加熱後經過濾碎渣而得一次濾液。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein in the step c), the pulverized liquid containing the slag of the scorpion saplings is subjected to the first microwave heating extraction, and the microwave device can be used for heating for 2 minutes for heating. The filtrate was obtained by filtering the slag.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟d、e為相同的重複步驟,即將上次過濾的碎渣再加入適量溶劑進行微波加熱萃取,採用微波設備進行加熱2分鐘,於加熱後經過濾碎渣而得濾液。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein the steps d and e are the same repeated steps, that is, the last filtered slag is further added with an appropriate amount of solvent for microwave heating extraction, and heated by a microwave device for 2 minutes. After heating, the residue was filtered to obtain a filtrate.

本發明蟬花萃取液之萃取方法的較佳實施例,其中上述諸等步驟中所添加的溶劑可為水、或乙醇、或是50%的水混合50%的丙二醇。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein the solvent added in the above steps may be water, or ethanol, or 50% water mixed with 50% propylene glycol.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟f為將多次萃取的濾液混合,再經濾紙過濾雜質。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein the step f is to mix the filtrate extracted a plurality of times, and then filter the impurities through the filter paper.

本發明蟬花萃取液之萃取方法的較佳實施例,其中當溶劑選擇採用乙醇時,係於步驟f之後進行步驟f1真空減壓濃縮,即將過濾雜質的濾液於低溫40度c的減壓空間進行濃縮蒸發乙醇,讓濾液蒸發至水不溶雜質析出的狀態。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein when the solvent is selected to use ethanol, the step f1 is followed by vacuum and concentration under the step f1, that is, the filtrate of the impurity is filtered at a reduced pressure of 40 degrees c. Ethanol was concentrated and evaporated, and the filtrate was evaporated to a state in which water-insoluble impurities were precipitated.

本發明蟬花萃取液之萃取方法的較佳實施例,其中步驟g係將過濾雜質後的濾液,以微細孔隙進行物理濾菌處理,而獲得最後的蟬花萃取液。A preferred embodiment of the method for extracting the scented flower extract of the present invention, wherein the step g is a physical filtration treatment of the filtrate after filtering the impurities, and the final sassafras extract is obtained.

本發明蟬花萃取液之用途主要目的與功效,係由以下具體技術手段所達成:The main purpose and efficacy of the use of the scented flower extract of the present invention are achieved by the following specific technical means:

其主要經由上述萃取方法的步驟而獲得蟬花萃取液,所述蟬花萃取液應用添加於保養品,於塗抹在皮膚上具有可改善或控制濕疹、過敏、異味性皮膚炎、脂漏性皮膚炎反覆發作之功用,達到抗菌、抗發炎及修復皮膚的功效。It is mainly obtained through the steps of the above extraction method, and the scented flower extract is added to the skin care product, and can be applied to the skin to improve or control eczema, allergy, odor dermatitis, and lipid leakage. The function of dermatitis is repeated, achieving antibacterial, anti-inflammatory and skin repair effects.

本發明蟬花萃取液之用途的較佳實施例,其中所述蟬花萃取液添加於保養品中,其所添加的重量百分比為0.1%~10%。A preferred embodiment of the use of the scented flower extract of the present invention, wherein the scented flower extract is added to the skin care product in an amount of 0.1% to 10% by weight.

為令本發明所運用之技術內容、發明目的及其達成之功效有更完整且清楚的揭露,茲於下詳細說明之,並請一併參閱所揭之圖式及圖號:For a more complete and clear disclosure of the technical content, the purpose of the invention and the effects thereof achieved by the present invention, it is explained in detail below, and please refer to the drawings and drawings:

首先,本發明實際運用技術與手段,請參閱第一圖所示,為本發明蟬花萃取液之萃取方法及其用途之步驟流程示意圖,係經由下列步驟來達成,如下:First, the practical application techniques and means of the present invention, as shown in the first figure, are schematic diagrams of the steps of the extraction method of the sassafras extract and the use thereof, which are achieved by the following steps, as follows:

a)冷凍蟬花子實體-係採用生鮮的蟬花子實體於低溫下冷凍一定值時間;a) frozen scorpion fruit body-system is frozen at a low temperature for a certain period of time using a fresh scorpion fruit body;

b)物理粉碎-將冷凍後之蟬花子實體,加入溶劑並經由高速物理粉碎機進行粉碎處理,而得粉碎液;b) physical pulverization - the frozen scorpion fruit body, added to the solvent and pulverized by a high-speed physical pulverizer to obtain a pulverized liquid;

c)一次微波萃取-將含有蟬花子實體碎渣的粉碎液進行第一次微波加熱萃取,於加熱後經過濾碎渣而得一次濾液;c) one-time microwave extraction-the pulverized liquid containing the shards of the scorpion saplings is subjected to the first microwave heating extraction, and after heating, the slag is filtered to obtain a primary filtrate;

d)二次微波萃取-再將碎渣加入溶劑進行第二次微波加熱萃取,微波加熱2分鐘,於加熱後經過濾碎渣而得二次濾液;d) secondary microwave extraction - the slag is added to the solvent for the second microwave heating extraction, microwave heating for 2 minutes, after heating, the residue is filtered to obtain a secondary filtrate;

e)三次微波萃取-再將碎渣加入溶劑進行第三次微波加熱萃取,微波加熱2分鐘,於加熱後經過濾碎渣而得三次濾液;e) three times of microwave extraction - the slag is added to the solvent for the third microwave heating extraction, microwave heating for 2 minutes, after heating, the residue is filtered to obtain three filtrates;

f)濾紙過濾-收集步驟c、d、e所萃取出的濾液並加以混合,再經濾紙過濾雜質;f) filter paper filtration - collecting the filtrate extracted in steps c, d, e and mixing, and filtering the impurities through the filter paper;

g)孔隙過濾-將步驟f中過濾雜質後的濾液,以微細孔隙進行物理濾菌處理,而獲得蟬花萃取液者。g) Pore Filtration - The filtrate after the impurity is filtered in the step f is subjected to physical filtration treatment with fine pores to obtain a scented flower extract.

以上為蟬花萃取液主要的萃取步驟;然而,上述步驟a中為取用生鮮蟬花子實體,而蟬花固態子實體的栽培方式有多種,其栽培方式會因不同廠商製作而有不同步驟,而在文獻中也有揭露出各種栽培流程,在本發明中的蟬花固態子實體栽培過程非訴求重點,故於此列舉一可實施的步驟作為參考,以使其萃取步驟更為完整;說明如下:The above is the main extraction step of the scented flower extract; however, in the above step a, the fresh squid fruit body is taken, and the scented solid fruit body is cultivated in various ways, and the cultivation method is different according to different manufacturers. In the literature, various cultivation processes are also disclosed. In the present invention, the cultivation process of the solid fruit body of the silk flower is not the focus of the application, so an implementable step is cited as a reference to make the extraction step more complete; :

首先,採用野生蟬花經原種接種、液體菌種接種而可獲得液體菌種,同時準備培養基,以全有機食材作為培養基,並經高溫高壓滅菌,再將液體菌種接種於培養基,爾後,經由暗栽培幾天、亮栽培幾天之後,而完成蟬花固態子實體栽培,以獲得生鮮蟬花子實體。Firstly, the wild sassafras is inoculated with the original seed and inoculated with the liquid species to obtain the liquid strain, and the medium is prepared, and the whole organic food is used as the medium, and the liquid strain is inoculated into the medium by high temperature and high pressure, and then After dark cultivation for a few days and bright cultivation for a few days, the solid fruit body cultivation of the silk flower is completed to obtain the fresh silk flower fruit body.

當步驟a冷凍蟬花子實體,採用生鮮的蟬花子實體於低溫下冷凍一定值時間[其溫度可設定為零下10-30度c之間的低溫,其冷凍時間為8-24小時];使其蟬花子實體經由冷凍破壁方式,因於冷凍低溫下會改變物質體積(膨脹),讓蟬花細胞壁破裂,使其在蟬花子實體冷凍後萃取,讓活性成分更容易萃出;接著,步驟b物理粉碎,即將上述冷凍的蟬花子實體加入適量溶劑中進行物理粉碎,於此採用高速物理粉碎機將冷凍後的蟬花子實體進行粉碎,而粉碎時間數分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液。進一步所添加的溶劑係可採用水、或乙醇、或50%的水混合50%的丙二醇;然而,所選用的溶劑不同,其步驟也會有些許變化,以下再說明之。When step a freezes the flowering fruit body, the fresh squid fruit body is frozen at a low temperature for a certain period of time [the temperature can be set to a low temperature between 10-30 degrees c, and the freezing time is 8-24 hours]; The flowering fruit body is frozen and broken, because the volume of the substance (expansion) is changed at the low temperature, and the cell wall of the flower is broken, so that it is extracted after freezing, so that the active ingredient is more easily extracted; then, step b Physical pulverization, that is, the above-mentioned frozen scorpion fruit body is physically pulverized by adding an appropriate amount of solvent, and the frozen scorpion fruit body is pulverized by a high-speed physical pulverizer, and the pulverization time is several minutes, and the pulverized body material can be obtained after pulverization. The pulverized liquid of the slag. Further added solvent may be 50% propylene glycol mixed with water, or ethanol, or 50% water; however, the solvent used may vary somewhat, as described below.

請參閱第一圖所示,於溶劑選用水、或50%的水混合50%的丙二醇時,係在步驟b中加入適量的水、或50%的水混合50%的丙二醇於冷凍後的蟬花子實體進行物理粉碎,粉碎時間數分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液,接著,於步驟c一次微波萃取,係將含有蟬花子實體碎渣的粉碎液置入於微波設備加熱數分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,可得到第一次的萃取濾液;續,進行步驟d二次微波萃取,係將步驟c中所過濾剩餘的碎渣,再加入適量的水、或50%的水混合50%的丙二醇(溶劑)混合成粉碎液,之後,將其置入於微波設備加熱數分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,而得到第二次的萃取濾液;相同地,步驟e三次微波萃取也如同步驟d一樣,加入適量的水、或50%的水混合50%的丙二醇後微波加熱再過濾,得到第三次的萃取濾液;而其步驟f濾紙過濾,即將步驟c、d、e所萃取出的三次濾液收集並加以混合,之後,以濾紙過濾雜質,再利用步驟g孔隙過濾,以微細孔隙過濾設備進行物理濾菌處理,而獲得蟬花萃取液者。Please refer to the first figure. When 50% propylene glycol is mixed with water or 50% water, add appropriate amount of water or 50% water to mix 50% propylene glycol in step b. The flowering fruit body is physically pulverized, and the pulverization time is several minutes. After pulverization, the pulverized liquid containing the shards of the scorpion scorpion body can be obtained, and then, in step c, a microwave extraction is performed, and the pulverized liquid containing the shards of the scorpion body is placed in the microwave equipment. After heating for a few minutes, after heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain a first extraction filtrate; and, after step d, the second microwave extraction is performed, and the remaining residue filtered in the step c is The slag is further mixed with a proper amount of water or 50% water mixed with 50% propylene glycol (solvent) to form a pulverized liquid, and then placed in a microwave device for heating for several minutes. After heating, the pulverized liquid is passed through a filter cloth or The filter bag filters the slag to obtain a second extraction filtrate; similarly, step e three times of microwave extraction is also the same as step d, adding an appropriate amount of water, or 50% water mixed with 50% propylene glycol, and then heating and filtering by microwave. Got the third time The filtrate is taken; and the filter paper of step f is filtered, that is, the three filtrates extracted by the steps c, d, and e are collected and mixed, and then the impurities are filtered by the filter paper, and then the pore filtration is performed by the step g, and the physical filtration is performed by the microporous filtration device. The bacteria are treated, and the scented flower extract is obtained.

請參閱第二圖所示,當溶劑選用乙醇時,係在步驟b中加入適量乙醇於冷凍後的蟬花子實體進行物理粉碎,粉碎時間數分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液,接著,於步驟c一次微波萃取,係將含有蟬花子實體碎渣的粉碎液置入於微波設備加熱數分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,可得到第一次的萃取濾液;續,進行步驟d二次微波萃取,係將步驟c中所過濾剩餘的碎渣,再加入適量乙醇(溶劑)混合成粉碎液,之後,將其置入於微波設備加熱數分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,而得到第二次的萃取濾液;相同地,步驟e三次微波萃取也如同步驟d一樣,加入適量乙醇後微波加熱再過濾,得到第三次的萃取濾液;而其步驟f濾紙過濾,即將步驟c、d、e所萃取出的三次濾液收集並加以混合,之後,以濾紙過濾雜質;然而,利用乙醇萃取可極大化萃取活性成分,但因乙醇無法添加於後續的成品[保養品]中,因此,進一步再步驟f之後,進行步驟f1真空減壓濃縮,以將過濾雜質的濾液於低溫減壓空間進行濃縮蒸發乙醇,讓濾液蒸發至水不溶雜質析出的狀態,之後,再利用步驟g孔隙過濾,以微細孔隙過濾設備進行物理濾菌處理,而獲得蟬花萃取液者。Please refer to the second figure. When the solvent is ethanol, add the appropriate amount of ethanol in step b to physically pulverize the frozen scorpion fruit body. The pulverization time is several minutes. After pulverization, the smash containing the shards of the scorpion body can be obtained. The liquid is then subjected to microwave extraction in step c. The pulverized liquid containing the shards of the scorpion body is placed in a microwave device for heating for several minutes. After heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain a slag. The first extraction filtrate; continued, performing step d secondary microwave extraction, mixing the remaining slag filtered in step c, adding appropriate amount of ethanol (solvent) to form a pulverized liquid, and then placing it in the microwave equipment After heating for a few minutes, after heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain a second extraction filtrate; similarly, step e three times of microwave extraction is also the same as step d, adding appropriate amount of ethanol and then heating by microwave After filtering, the third extraction filtrate is obtained; and the step f filter paper is filtered, that is, the three filtrates extracted in steps c, d, and e are collected and mixed, and then filtered with filter paper. However, extraction with ethanol can maximize the extraction of the active ingredient, but since ethanol cannot be added to the subsequent finished product [care product], after further step f, the step f1 is vacuum-concentrated and concentrated to filter the impurity filtrate. Ethanol is concentrated and concentrated in a low-temperature decompression space, and the filtrate is evaporated to a state in which water-insoluble impurities are precipitated. Thereafter, the pores are filtered by a step g, and the microfiltration device is used for physical filtration treatment to obtain a scented flower extract.

藉此,本發明利用冷凍後可以使得蟬花細胞壁破裂,讓活性成分容易萃出,且冷凍後進行物理粉碎與微波萃取階段,溫度皆能控制小於40度c,低溫微波萃取速度快且可以降低蟲草素,腺苷,HEA及SOD酵素等高溫質變機率並增加萃取液活性。Thereby, the invention can utilize the freezing to make the cell wall of the silk flower rupture, and the active ingredient can be easily extracted, and the physical pulverization and microwave extraction stage after freezing can control the temperature less than 40 degrees c, and the low temperature microwave extraction speed is fast and can be reduced. The high temperature change rate of cordycepin, adenosine, HEA and SOD enzymes increases the activity of the extract.

以下列舉二個實際萃取過程之實施例,加以說明:The following is an example of two actual extraction processes, which are illustrated:

實施例一為選用水作為添加溶劑In the first embodiment, water is used as an additive solvent.

選用1公斤蟬花子實體,於零下30度c的低溫下冷凍時間12小時[可達到良好破壁效果及活性萃取],讓蟬花細胞壁於冷凍過程因體積改變的因素而破裂;將冷凍後蟬花子實體進行萃取,而其冷凍後狀態會讓活性成分更容易萃出[呈現出水狀態],再將冷凍後的蟬花子實體置放於1000W轉速30000rpm的高速粉碎機中,並加入500cc的水[溶劑]進行物理粉碎,粉碎時間2分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液。接著,將含有蟬花子實體碎渣的粉碎液置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,可得到第一次的萃取濾液;續,再將上述過濾剩餘的碎渣加入1000cc的水[溶劑]混合成粉碎液,同樣置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,而得到第二次的萃取濾液;相同地,再重複上述步驟一次,得到第三次的萃取濾液,再來將所萃取出的三次濾液收集並加以混合,總量約3000cc[包含一、二、三次萃取所添加的水及蟬花子實體釋出的活性成分與水份],將3000cc的混合濾液以濾紙初步過濾雜質,再利用微細0.2μm孔隙[為最佳]的過濾設備進行物理濾菌處理,最後,可獲得2500cc蟬花萃取液者。1 kg of safflower fruit body is used, and the freezing time is 12 hours at a low temperature of minus 30 degrees C [a good wall breaking effect and active extraction can be achieved], and the cell wall of the scorpion flower is broken due to the volume change factor in the freezing process; The flowering fruit body is extracted, and its frozen state makes the active ingredient more easily extracted [presenting the water state], and then the frozen scorpion body is placed in a high-speed pulverizer of 1000 W rotation speed of 30,000 rpm, and 500 cc of water is added [ The solvent was subjected to physical pulverization, and the pulverization time was 2 minutes, and after pulverization, a pulverized liquid containing shards of the scorpion body was obtained. Next, the pulverized liquid containing the shards of the scorpion saplings is placed in a microwave oven for heating for 2 minutes, and after heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain the first extraction filtrate; The slag remaining in the above filtration was added to 1000 cc of water [solvent] to be mixed into a pulverized liquid, and the mixture was placed in a microwave oven for heating for 2 minutes. After heating, the pulverized liquid was filtered through a filter cloth or a filter bag to obtain a pulverized residue. The second extraction filtrate; similarly, the above steps are repeated once more to obtain a third extraction filtrate, and the extracted three filtrates are collected and mixed for a total amount of about 3000 cc [including one, two, three extractions The added water and the active ingredient released from the flowering fruit body and the water], the 3000 cc mixed filtrate is initially filtered with impurities on the filter paper, and then subjected to physical filtration treatment using a fine 0.2 μm pore [optimal] filtration device, and finally , 2500cc scented flower extract can be obtained.

實施例二為選用乙醇作為添加溶劑The second embodiment is the use of ethanol as an additive solvent.

選用1公斤蟬花子實體,於零下30度c的低溫下冷凍時間12小時,讓蟬花細胞壁於冷凍過程因體積改變的因素而破裂;將冷凍後蟬花子實體進行萃取,而其冷凍破壁狀態會讓活性成分更容易萃出[呈現出水狀態],將冷凍後的蟬花子實體置放於1000W轉速30000rpm的高速粉碎機中,並加入500cc濃度75%的乙醇[溶劑]進行物理粉碎,粉碎時間2分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液。接著,將含有蟬花子實體碎渣的粉碎液置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,可得到第一次的萃取濾液;續,再將上述過濾剩餘的碎渣加入500cc濃度75%的乙醇[溶劑]混合成粉碎液,同樣置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,而得到第二次的萃取濾液;相同地,再重複上述步驟一次,得到第三次的萃取濾液,再來將所萃取出的三次濾液收集並加以混合,總量約2000cc[包含一、二、三次萃取所添加的水及蟬花子實體釋出的活性成分與水份],將2000cc的混合濾液以濾紙初步過濾雜質,再於低溫40度c的減壓空間進行濃縮蒸發乙醇,讓濾液蒸發至剩餘500cc[乙醇完全蒸發],將蒸發乙醇後的濾液,利用微細0.2μm孔隙的過濾設備進行物理濾菌處理,最後,可獲得450cc蟬花萃取液者。1 kg of safflower fruit body was used, and the freezing time was 12 hours at a low temperature of minus 30 degrees c, so that the cell wall of the scorpion flower was ruptured due to the volume change factor during the freezing process; the frozen scorpion fruit body was extracted and frozen and broken state. The active ingredient is more easily extracted [presents a water state], and the frozen scorpion body is placed in a high-speed pulverizer of 1000 W rotation speed of 30,000 rpm, and 500 cc of 75% ethanol [solvent] is added for physical pulverization, pulverization time. After 2 minutes, the pulverized liquid containing the scum of the scorpion body body was obtained after pulverization. Next, the pulverized liquid containing the shards of the scorpion saplings is placed in a microwave oven for heating for 2 minutes, and after heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain the first extraction filtrate; The slag remaining in the above filtration is added to a mixture of 500 cc of 75% ethanol [solvent] to form a pulverized liquid, which is also placed in a 700 W microwave apparatus for heating for 2 minutes. After heating, the pulverized liquid is filtered through a filter cloth or a filter bag. And obtaining the second extraction filtrate; similarly, repeating the above steps once again, obtaining the third extraction filtrate, and collecting and extracting the extracted three filtrates, the total amount is about 2000 cc [including one or two And three times of extraction of the added water and the active ingredient released from the flowering body and water], the 2000 cc of the mixed filtrate is initially filtered with impurities on the filter paper, and then concentrated and evaporated in a reduced pressure space of 40 ° C to evaporate the filtrate. To the remaining 500 cc [evaporation of ethanol], the filtrate after evaporation of ethanol was subjected to physical filtration treatment using a fine 0.2 μm pore filtration device, and finally, 450 cc of anthraquinone extract was obtained.

實施例三為選用水混合丙二醇作為添加溶劑The third embodiment is the use of water mixed propylene glycol as an additive solvent.

選用1公斤蟬花子實體,於零下30度c的低溫下冷凍時間12小時[可達到良好破壁效果及活性萃取],讓蟬花細胞壁於冷凍過程因體積改變的因素而破裂;將冷凍後蟬花子實體進行萃取,而其冷凍破壁狀態會讓活性成分更容易萃出[呈現出水狀態],再將冷凍後的蟬花子實體置放於1000W轉速30000rpm的高速粉碎機中,並加入由重量百分比50%水混合重量百分比50%丙二醇的500cc溶劑進行物理粉碎,粉碎時間2分鐘,粉碎後可得到含有蟬花子實體碎渣的粉碎液。接著,將含有蟬花子實體碎渣的粉碎液置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,可得到第一次的萃取濾液;續,再將上述過濾剩餘的碎渣加入重量百分比50%水混合重量百分比50%丙二醇的1000cc的溶劑混合成粉碎液,同樣置入於700W微波設備加熱2分鐘,於加熱後,將粉碎液經由濾布或濾袋過濾碎渣,而得到第二次的萃取濾液;相同地,再重複上述步驟一次,得到第三次的萃取濾液,再來將所萃取出的三次濾液收集並加以混合,總量約3000cc[包含一、二、三次萃取所添加的水及蟬花子實體釋出的活性成分與水份],將3000cc的混合濾液以濾紙初步過濾雜質,再利用微細0.2μm孔隙[為最佳]的過濾設備進行物理濾菌處理,最後,可獲得2500cc蟬花萃取液者。然而,此實施例採用水混合丙二醇主要優點為丙二醇能如同乙醇一樣,將水所不能萃出來的成分一起萃出來,同時,丙二醇並不需經過真空減壓濃縮把酒精揮發掉的優點功效。1 kg of safflower fruit body is used, and the freezing time is 12 hours at a low temperature of minus 30 degrees C [a good wall breaking effect and active extraction can be achieved], and the cell wall of the scorpion flower is broken due to the volume change factor in the freezing process; The flowering fruit body is extracted, and its frozen and broken state makes the active ingredient more easily extracted [presenting the water state], and then the frozen scorpion body is placed in a high speed pulverizer of 1000 W rotating speed of 30,000 rpm, and added by weight percentage 50% water was mixed with 500 cc of 50% propylene glycol to carry out physical pulverization, and the pulverization time was 2 minutes. After pulverization, a pulverized liquid containing shards of scorpion body solids was obtained. Next, the pulverized liquid containing the shards of the scorpion saplings is placed in a microwave oven for heating for 2 minutes, and after heating, the pulverized liquid is filtered through a filter cloth or a filter bag to obtain the first extraction filtrate; Then, the remaining slag of the above filtration is added to a 1000 cc solvent of 50% by weight of water and 50% by weight of propylene glycol to be mixed into a pulverized liquid, and the mixture is also placed in a microwave apparatus for heating for 2 minutes in a microwave oven for 2 minutes. After heating, the pulverized liquid is passed through a filter cloth. Or filter bag to filter the slag to obtain a second extraction filtrate; similarly, repeat the above steps once to obtain a third extraction filtrate, and then collect and extract the extracted three filtrates, the total amount is about 3000cc [containing the water added by the first, second and third extractions and the active ingredient and water released by the flowering fruit body], the 3000 cc mixed filtrate is initially filtered with impurities on the filter paper, and then the fine 0.2 μm pores are used [best] The filtration equipment is subjected to physical filtration treatment, and finally, 2500 cc of scented flower extract is obtained. However, the main advantage of using water-mixed propylene glycol in this embodiment is that propylene glycol can extract together the components which cannot be extracted by water like ethanol, and at the same time, propylene glycol does not need to be vacuum-concentrated and concentrated to volatilize the alcohol.

經由上述萃取方法所提煉的蟬花萃取液能應用添加於化妝水、精華液、面膜或乳液等保養品;於塗抹在皮膚上具有可改善或控制濕疹、過敏、異味性皮膚炎、脂漏性皮膚炎反覆發作之功用,達到抗菌、抗發炎及修復皮膚的功效。其中所述蟬花萃取液添加於保養品中,其添加比例為0.1%~10%。The scented flower extract extracted by the above extraction method can be applied to skin care products such as lotion, essence, mask or lotion; it can be used on the skin to improve or control eczema, allergies, odorous dermatitis, and lipid leakage. Sexual dermatitis has the effect of recurring episodes, achieving antibacterial, anti-inflammatory and skin repairing effects. The silk flower extract is added to the skin care product, and the addition ratio thereof is 0.1% to 10%.

一般因皮膚表面屏障功能差,容易經由異物入侵而產生皮膚炎,傳統方式為塗抹類固醇,而塗抹類固醇會讓細胞變小,使得細胞間的脂質變少,因此,塗抹類固醇雖能抑制發炎反應,但卻具有讓皮膚變薄的副作用,同時對於角質層的防禦功能造成嚴重影響;而本發明中所萃取之蟬花萃取液中含有具抗菌功效的蟲草素、白僵菌素、腺苷,及含有抗發炎的麥角固醇過氧化物,及含有止癢鎮痛效果的HEA成分,及可調節免疫系統的ISP-1成分,能有效改善皮膚發炎症狀;於塗抹在皮膚之後,並無類固醇使得皮膚變薄的副作用。Generally, due to poor skin surface barrier function, skin inflammation is easily caused by foreign body invasion. The traditional method is to apply steroids, and smearing steroids will make the cells smaller, so that the lipids between cells become less. Therefore, although smear can inhibit inflammatory reaction, However, it has the side effect of thinning the skin, and at the same time has a serious effect on the defense function of the stratum corneum; and the extract of the scented flower extracted in the present invention contains cordycepin, beauverin, adenosine having antibacterial effect, and It contains anti-inflammatory ergosterol peroxide, HEA with anti-itch analgesic effect, and ISP-1 which can regulate the immune system. It can effectively improve the symptoms of skin inflammation. After application to the skin, there is no steroid. Side effects of thinning of the skin.

然而前述之實施例或圖式並非限定本發明之產品結構或使用方式,任何所屬技術領域中具有通常知識者之適當變化或修飾,皆應視為不脫離本發明之專利範疇。However, the above-described embodiments or drawings are not intended to limit the structure or the use of the present invention, and any suitable variations or modifications of the invention will be apparent to those skilled in the art.

藉由以上所述,本發明系統之組成與使用實施說明可知,本發明與現有結構相較之下,具有下列優點:From the above, the composition and use description of the system of the present invention show that the present invention has the following advantages as compared with the existing structure:

1.本發明藉由採用蟬花子實體來製作萃取,能由子實體中獲得更高的活性成分。1. The present invention produces a higher active ingredient from a fruiting body by using a flowering fruit body to make an extract.

2.本發明藉由蟬花子實體經由冷凍破壁方式,使其在冷凍後可以使得蟬花細胞壁破裂,讓活性成分更容易萃出。2. The present invention allows the active constituents to be more easily extracted by freezing the wall of the flowering body to cause the cell wall of the silk flower to rupture after freezing.

3.本發明萃取方法,藉由於物理粉碎與微波萃取階段,讓溫度控制小於40度C以下,在低溫微波萃取速度快,且可以降低蟲草素、腺苷、HEA及SOD酵素等高溫質變機率並增加萃取液活性的功效。3. The extraction method of the invention, by the physical pulverization and microwave extraction stage, the temperature control is less than 40 degrees C, the microwave extraction speed at low temperature is fast, and the high temperature change probability of cordycepin, adenosine, HEA and SOD enzyme can be lowered and Increase the effectiveness of extract activity.

4.本發明應用於保養品的用途,能改善或控制濕疹、過敏、異味性皮膚炎、脂漏性皮膚炎反覆發作之功用,達到抗菌、抗發炎及修復皮膚的功效,且無副作用。4. The invention is applied to the use of the skin care product, can improve or control the functions of eczema, allergy, odor dermatitis, and lipid-lowering dermatitis, and achieve antibacterial, anti-inflammatory and skin repair effects without side effects.

綜上所述,本發明實施例確能達到所預期之使用功效,又其所揭露之具體構造,不僅未曾見諸於同類產品中,亦未曾公開於申請前,誠已完全符合專利法之規定與要求,爰依法提出發明專利之申請,懇請惠予審查,並賜准專利,則實感德便。In summary, the embodiments of the present invention can achieve the expected use efficiency, and the specific structure disclosed therein has not been seen in similar products, nor has it been disclosed before the application, and has completely complied with the provisions of the Patent Law. And the request, the application for the invention of a patent in accordance with the law, please forgive the review, and grant the patent, it is really sensible.

(a)‧‧‧冷凍蟬花子實體(a) ‧‧‧Frozen scorpion fruiting bodies

(b)‧‧‧物理粉碎(b) ‧‧‧Physical smash

(c)‧‧‧一次微波萃取(c) ‧‧‧A microwave extraction

(d)‧‧‧二次微波萃取(d) ‧ ‧ secondary microwave extraction

(e)‧‧‧三次微波萃取(e) ‧ ‧ three microwave extractions

(f)‧‧‧濾紙過濾(f) ‧ ‧ filter paper filtration

(f1)‧‧‧真空減壓濃縮(f1)‧‧‧ Vacuum decompression and concentration

(g)‧‧‧孔隙過濾(g) ‧ ‧ pore filtration

第一圖:本發明之步驟流程示意圖一。First figure: Schematic diagram 1 of the flow chart of the present invention.

第二圖:本發明之步驟流程示意圖二。Second figure: Schematic diagram 2 of the flow chart of the present invention.

Claims (12)

一種蟬花萃取液之萃取方法,其步驟為:   a)冷凍蟬花子實體-係採用生鮮的蟬花子實體於低溫下冷凍一定值時間;   b)物理粉碎-將冷凍後之蟬花子實體加入溶劑並經由高速物理粉碎設備進行粉碎處理,而得粉碎液;   c)一次微波萃取-將含有蟬花子實體碎渣的粉碎液進行第一次微波加熱萃取,於加熱後經過濾碎渣而得一次濾液;   d)二次微波萃取-再將碎渣加入溶劑進行第二次微波加熱萃取,於加熱後經過濾碎渣而得二次濾液;   e)三次微波萃取-再將碎渣加入溶劑進行第三次微波加熱萃取,於加熱後經過濾碎渣而得三次濾液;   f)濾紙過濾-收集步驟c、d、e所萃取出的濾液並加以混合,再經濾紙過濾雜質;   g)孔隙過濾-將步驟f中過濾後的濾液以微細孔隙過濾設備進行物理濾菌處理,而獲得蟬花萃取液者。A method for extracting a sassafras extract, the steps of which are: a) freezing the scorpion body--using a fresh squid fruit body to freeze at a low temperature for a certain period of time; b) physically pulverizing - adding the frozen scorpion fruit body to the solvent and The pulverization treatment is carried out through a high-speed physical pulverizing apparatus to obtain a pulverizing liquid; c) one-time microwave extraction--the pulverized liquid containing the shards of the scorpion saplings is subjected to the first microwave heating extraction, and after heating, the slag is filtered to obtain a primary filtrate; d) secondary microwave extraction - the slag is added to the solvent for the second microwave heating extraction, and after heating, the slag is filtered to obtain a secondary filtrate; e) three times of microwave extraction - the slag is added to the solvent for the third time Microwave heating extraction, after heating, the residue is filtered to obtain three filtrates; f) filter paper filtration - collecting the filtrate extracted in steps c, d, e and mixing, and filtering the impurities through the filter paper; g) pore filtration - the steps The filtered filtrate of f is subjected to physical filtration treatment by a microporous filtration device to obtain a scented flower extract. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中步驟a中冷凍溫度控制於零下10-30度c的低溫,且冷凍時間為8-24小時。The extraction method of the scented flower extract as described in claim 1, wherein the freezing temperature in the step a is controlled at a low temperature of minus 10-30 degrees C, and the freezing time is 8-24 hours. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中步驟b中高速物理粉碎設備之轉速為30000rpm進行物理粉碎,粉碎時間2分鐘。The extraction method of the scented flower extract solution according to the first aspect of the patent application, wherein the high-speed physical pulverization apparatus in step b is physically pulverized at a rotation speed of 30,000 rpm, and the pulverization time is 2 minutes. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中步驟b中的溶劑係採用水。The extraction method of the scented flower extract as described in claim 1, wherein the solvent in the step b is water. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中步驟b中的溶劑係採用50%的水混合50%的丙二醇。The extraction method of the sassafras extract according to claim 1, wherein the solvent in the step b is 50% propylene glycol mixed with 50% water. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中步驟b中的溶劑係採用乙醇,且為濃度75%的乙醇。The extraction method of the sassafras extract as described in claim 1, wherein the solvent in the step b is ethanol and is 75% ethanol. 如申請專利範圍第1項所述之蟬花萃取液之萃取方法,其中於步驟g中係採用微細0.2-0.5μm孔隙的過濾設備進行物理濾菌處理。The extraction method of the scented flower extract solution according to the first aspect of the patent application, wherein in the step g, the filtration treatment device with a fine 0.2-0.5 μm pore is used for physical filtration treatment. 如申請專利範圍第6項所述之蟬花萃取液之萃取方法,其中溶劑為乙醇時係於步驟f之後,進行步驟f1真空減壓濃縮,以將過濾雜質的濾液於低溫減壓空間進行濃縮蒸發乙醇,讓濾液蒸發至水不溶雜質析出的狀態。The extraction method of the scented flower extract solution as described in claim 6 wherein the solvent is ethanol is after step f, and the step f1 is vacuum-concentrated and concentrated to concentrate the filtrate of the filtered impurities in a low-temperature decompression space. The ethanol was evaporated, and the filtrate was evaporated to a state in which water-insoluble impurities were precipitated. 如申請專利範圍第8項所述之蟬花萃取液之萃取方法,其中於步驟g中係採用微細0.2-0.5μm孔隙的過濾設備進行物理濾菌處理。The method for extracting anthraquinone extract according to claim 8 of the patent application, wherein in step g, the filtration treatment device using a fine 0.2-0.5 μm pore is used for physical filtration treatment. 如申請專利範圍第9項所述之蟬花萃取液之萃取方法,其中步驟f1中以低溫40度c的減壓空間進行濃縮蒸發乙醇。The extraction method of the scented flower extract solution according to claim 9, wherein in step f1, the ethanol is concentrated and evaporated at a reduced pressure of 40 degrees C. 一種含有如申請專利範圍第1至10項中任一項之含有蟬花萃取液之保養品。A skin care product comprising a scented flower extract as claimed in any one of claims 1 to 10. 如申請專利範圍第11項所述之含有蟬花萃取液之保養品,其中所述蟬花萃取液添加的重量百分比為0.1%~10%。The skin care product containing the scented flower extract according to claim 11, wherein the sassafras extract is added in a weight percentage of 0.1% to 10%.
TW107103098A 2018-01-29 2018-01-29 Extracting method of cordyceps cicadae extract and the purpose thereof TW201932133A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113480587A (en) * 2021-05-20 2021-10-08 徐州工程学院 Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113480587A (en) * 2021-05-20 2021-10-08 徐州工程学院 Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp
CN113480587B (en) * 2021-05-20 2024-05-03 湖南合健生物科技有限公司 Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies

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