TW201814274A - Optical detection system - Google Patents

Abstract

An optical detection system includes a light emitting module, a test strip and a receiving module. The light emitting module includes a light source and a first light shielding unit, and the light source provides a light beam. The first light shielding unit has a first aperture which disposed corresponding to the light source. A test strip includes a cassette and a test paper. The cassette has a first window, a second window and a sample opening, and the sample opening is disposed on one surface of the cassette. The first window is disposed corresponding to the second window, and the first window and the second window are disposed respectively on the opposite sides of the cassette. The test paper is set in the cassette. The receiving module includes a second light shielding unit and an optical sensor. The second light shielding unit has a second aperture, and the second aperture is disposed corresponding to the second window. The optical sensor receives the light beam and sends a measurement signal.

Description

Optical detection system

The invention relates to an optical detection system, in particular to an optical detection system for detecting a test strip.

The current Lateral Flow Assay (LFA) technology is widely used in fast screening detection related fields due to its convenient use and mature process technology, and its detection results are in addition to visual judgment, reflective optical detection and complementary Complementary Metal-Oxide Semiconductor (CMOS) image capture devices are also common interpretation technologies. However, when using the naked eye to judge the detection results, in addition to the differences in human judgment, the weak response to less obvious color rendering often leads to misjudgment by the user; while the reflective optical detection technology can only detect the test strip The color change on the surface of the strip cannot reflect the detection of the color change hidden inside the fiber of the test strip. In addition, the reflective optical detection signal is easily affected by the change in the distance between the surface of the test strip and the optical reading device. The measurement variation is large and requires precise mechanism cooperation, so it cannot be applied to the requirements of portable detection.

In addition, the CMOS image capture device can capture the image of the fast screening test strip through the camera, and then use the image analysis technology to circle the color or brightness of the specific reaction block image and quantify it. Although the problem of visual judgment is solved, but The sensitivity limit of its sensitivity has not improved significantly. Therefore, how to improve the convenience and sensitivity of the detection instrument has become a major issue in the development of the test strip reading device.

In view of the above problems, the object of the present invention is to provide a penetrating optical detection system to reduce the complexity of the design of the reading device and improve the reliability of the detection. It can also sense the reaction signal hidden inside the test strip, thereby improving Detection sensitivity.

To achieve the above object, an optical detection system according to the present invention includes a light emitting module, a detection test piece, and a receiving module. The light emitting module includes a light source and a first light shielding unit, and the light source provides a light beam. The first shading unit has a first aperture, and the first aperture is disposed corresponding to the light source. The test strip includes a cassette and a test strip. The cassette has a first window, a second window, and a specimen opening. The specimen opening is disposed on a surface of the cassette. The first window and the second window are correspondingly arranged and are respectively opened on opposite sides of the cassette. A window is disposed corresponding to the first aperture. The test strip is set in the cassette. The receiving module includes a second shading unit and an optical sensor. The second light shielding unit has a second aperture, and the second aperture is disposed corresponding to the second window. The optical sensor is used for receiving a light beam and sending a measurement signal. After the light beam exits the first light-shielding unit through the first aperture, the light beam sequentially penetrates the first window, the test strip, and the second window and enters the second light-shielding unit through the second aperture.

In one embodiment, the test strip includes at least a test strip and a quality control strip, and the test strip and the quality control strip are distributed in an intersection range of a vertical projection surface of the first window and the second window on the test strip.

In one embodiment, the diameter of the first pore is less than or equal to the width of the test strip and the width of the quality control strip.

In one embodiment, the diameter of the second pore is smaller than or equal to the diameter of the first pore.

In one embodiment, the diameter of the first pore is between 0.1 and 5.0 mm.

In an embodiment, the light source is a light-emitting diode, and the test strip further has a color-producing material, and the light wavelength emitted by the light-emitting diode is the wavelength of light absorbed by the color-producing material.

In one embodiment, the light source, the first aperture, the second aperture, and the optical sensor together form an optical detection path, and the optical detection path is substantially perpendicular to the test strip.

In one embodiment, it further includes a test piece moving device for fixing and driving the test piece to move linearly along the long axis direction of the first window, so that the light beam is irradiated to a part of the cassette and the first window along the long axis direction .

In one embodiment, the test strip moving device is an automatic driving device or a manual driving device.

In one embodiment, the automatic driving device includes a transmission device such as a slide rail, a screw, a gear or a belt, and is connected to a motor.

In one embodiment, the manual driving device is designed with a slotted slider and a slotted groove, and a finger moves directly to detect the test piece for linear motion.

In one embodiment, a signal analysis module is further included. The signal analysis module includes a signal analysis unit and a signal calculation unit. The signal analysis unit receives the measurement signal and outputs a parameter according to the measurement signal, wherein the parameter is a background signal parameter, a quality control signal parameter, a test signal parameter, or a first window time parameter. The signal calculation unit uses at least one parameter to perform calculation to estimate a specific substance concentration in the test object.

In one embodiment, the optical detection system measures a detection signal hidden inside the fiber of the test strip through a penetrating optical detection path.

As mentioned above, the optical detection system of the present invention measures the detection signal hidden in the fiber of the test strip through the penetrating optical detection path to enhance the intensity of the detection signal; the scanning design of the optical detection path is used to The long axis of the first window of the test piece sequentially scans the blank areas, test strips and quality control strips of the test strip in the first window in order to reduce the number of optical sensors and reduce the complexity of the design of the reading device. It also increases the flexibility of increasing and decreasing the number of test strips on the test strips, while reducing the precision requirements for drawing test strips and quality control strips. Finally, the cooperation of the first pore and the second pore is used to increase the reliability of the measurement signal, improve the signal-to-noise ratio and intensity of the measurement signal, and thereby achieve the purpose of improving the convenience and sensitivity of the detection instrument.

Hereinafter, an optical detection system according to an embodiment of the present invention will be described with reference to related drawings. The same components will be described with the same reference symbols.

Please refer to FIG. 1, FIG. 2, FIG. 3A and FIG. 3B simultaneously. FIG. 1 is a schematic diagram of an optical detection system according to an embodiment of the present invention, and FIG. 2 is a schematic diagram of an optical detection path according to an embodiment of the present invention. Therefore, the cassette of the test strip is omitted. FIG. 3A is a schematic perspective view of the test strip according to an embodiment of the present invention, and FIG. 3B is an explosion schematic view of the test strip shown in FIG. 3A.

The present invention provides an optical detection system OD including a light emitting module 1, a test strip 2 and a receiving module 3. The light-emitting module 1 includes a light source 11 and a first light-shielding unit 12. The light source 11 provides a light beam 111, and the first light-shielding unit 12 has a first aperture 121, and the first aperture 121 is arranged corresponding to the light source 11 so that the light beam 111 The first light shielding unit 12 is emitted through the first aperture 121.

The test strip 2 includes a cassette 21 and a test strip 22, wherein the cassette 21 has a first window 211, a second window 212, and a specimen opening 213. The specimen opening 213 is disposed on the cassette 21 On one surface, the first window 211 and the second window 212 are disposed correspondingly and are respectively disposed on opposite sides of the cassette 21, the first window 211 is disposed correspondingly to the first aperture 121, and the test strip 22 is disposed in the cassette 21, After the light beam 111 is emitted from the first light-shielding unit 12, the light beam 111 penetrates the first window 211 and the test strip 22 in sequence, and exits the test strip 2 through the second window 212.

The receiving module 3 includes a second shading unit 31 and an optical sensor 32. The second light-shielding unit 31 has a second aperture 311, and the second aperture 311 is arranged corresponding to the second window. After the light beam 111 exits the test strip, it enters the second light-shielding unit 31 through the second aperture 311, and optically The sensor 32 is used for receiving the light beam 111 and sending a measurement signal. After the light beam 111 exits the first light shielding unit 12 through the first aperture 121, the light beam 111 penetrates the first window 211, the test strip 22, and the second window 212 in sequence and enters the second light shielding unit 31 through the second aperture 311.

The optical detection system provided by the present invention measures a detection signal hidden inside a test strip fiber through a penetrating optical detection path. After the light beam exits the first light-shielding unit through the first aperture, the light beam sequentially penetrates the first The window, the test strip, and the second window are incident into the second light shielding unit through the second aperture. Therefore, in this embodiment, the light source 11, the first aperture 121, the second aperture 311, and the optical sensor 32 together constitute an optical detection path OP, and the optical detection path OP is substantially perpendicular to the test strip 2. The detection reaction area of the test strip 2 is a hollow design, so the test strip 2 has a first window 211 and a second window 212 corresponding to each other. The test strip 22 includes at least one test strip T and one quality control strip C, and The test strip T and the quality control strip C are distributed at the intersection of the vertical projection surface of the first window 211 and the second window 212 on the test strip 22, in other words, the test strip T and the quality control strip on the test strip 22 In the area of C distribution, after the light beam 111 is emitted from the light emitting module 1, it can be incident on the first window 211, penetrate the test strip T or the quality control band C on the test strip 22, and finally exit the inspection through the second window 212 The test piece 2 enables the detection signal of the test zone T or the quality control zone C to be received by the receiving module 3.

In this embodiment, when the diameter of the first aperture 121 is between 0.1 and 5.0 mm, and the diameter of the first aperture 121 is less than or equal to the width of the test strip T and the width of the quality control strip C, the light beam 111 can be transmitted through When the test zone T or the quality control zone C, the width of the beam 111 is smaller than the width of the test zone T and the quality control zone C. In addition, the diameter of the second aperture 311 is smaller than the diameter of the first aperture 121, which enables optical sensing. When the receiver 32 receives the light beam 111, excessive receiver noise can be filtered out to improve the reliability of the measurement signal.

With the design of the light-transmitting optical detection path, the positions of the light source and the optical sensor are fixed, so the detection signal is not easily affected by the change in the distance between the test strip and the optical sensor, and the diameter of the first aperture is less than or equal to The width of the test strip and the quality control strip, and the diameter of the second aperture is smaller than or equal to the diameter of the first aperture, which can reduce the problem of uneven light source intensity. No optical diffuser is required for detection, and only a single optical sensor is needed. The measuring device can make the mechanism of the testing instrument more streamlined, and it can also simplify the measurement and calibration operation.

In this embodiment, the optical detection system OD further includes a test strip moving device 4 for fixing and driving the test strip 2 to move linearly along the long axis direction MA _{211 of} the first window 211 to make the light beam MA ₂₁₁ along the major axis is irradiated to the first cartridge 21 and the window portion 211. To further explain, the test piece moving device 4 is used to drive the test piece 2 and the optical detection path OP at a fixed position to generate a linear relative movement, so that the optical detection path OP is sequentially irradiated to a part of the first window 211 in the long axis direction MA ₂₁₁ . The cassette 21, the blank block 221 of the test strip 22 in the first window 211, the test strip T and the quality control strip C, and then the blank block 221 of the test strip 22 is irradiated, and finally the optical detection path OP leaves the first After the window 211 is irradiated to a part of the cassette 21, the test strip 2 stops moving.

In this embodiment, the test piece moving device 4 is an automatic driving device or a manual driving device; the automatic driving device is a transmission device such as a slide rail, a screw, a gear, or a belt, and is connected to a motor (not shown in the figure). Make the test strip 2 move linearly along the long axis direction MA _{211 of} the first window 211, and the manual driving device is designed with a slotted slider and a slide groove (not shown in the figure), and the test strip 2 is directly moved by the finger. Make a linear motion.

By scanning the optical detection path, the test piece and the optical detection path generate linear relative movement, so that the optical detection path sequentially scans each test strip and quality control strip on the test strip, which can overcome the process variation and product compatibility. The problem of displacement of the optical sensor and the test strip can further reduce the precision requirements for the production of the test strip, and increase the flexibility of increasing or decreasing the number of test strips.

Please refer to FIGS. 4, 5 and 6 at the same time. FIG. 4 is a schematic diagram of a potential waveform of an output measurement signal according to an embodiment of the present invention. FIG. 5 is a schematic diagram of a potential waveform of an output measurement signal according to another embodiment of the present invention. 6 is a schematic diagram of a test strip signal-to-noise ratio according to an embodiment of the present invention.

In an embodiment, the light source 11 is a light-emitting diode, and the test strip 22 further has a color-emitting material 222. The wavelength of light emitted by the light-emitting diode is the wavelength of light absorbed by the color-emitting material 222, and the color-emitting material 222 is coated on each test strip T and quality control strip C on the test strip 22. Therefore, when the light beam 111 passes through each test zone T or quality control zone C, part of the light wavelength will be absorbed by the coloring material 222, which weakens the light intensity of the light beam 111. After the optical sensor 32 receives the light intensity change of the light beam 111 , In response to the output measurement signal.

In one embodiment, the optical detection system OD further includes a signal analysis module. The signal analysis module includes a signal analysis unit and a signal calculation unit. The signal analysis unit receives the measurement signal and outputs a parameter according to the measurement signal, wherein the parameter is a background signal parameter, a quality control signal parameter, a test signal parameter, or a first window time ΔA parameter. A signal calculation unit uses at least one parameter for calculation and outputs a signal-to-noise ratio.

The operation modes of the signal analysis module of this embodiment will be described below using FIG. 4, FIG. 5, and FIG. 6.

When the optical detection path OP sequentially scans the test strip 2 along the long axis direction MA _{211 of} the first window 211, the potential waveform of the output measurement signal is obtained as shown in FIG. 4, and the horizontal axis is detected by the optical detection path OP. Sampling time, the vertical axis is the voltage of the measurement signal. Since the cassette 21 of the test strip 2 is opaque, the measurement signal detected by the optical detection path OP is close to zero. As the test strip 2 continues to advance, when the optical test path OP starts to enter the first In the window 211, when the light beam 111 passes through the blank block 221 of the light-transmitting test strip 22, the measurement signal increases sharply, and its time axis is set to zero. Thereafter, when the test strip T and the quality control strip C respectively pass through the optical detection path OP, part of the light wavelength is absorbed by the coloring material 222, and the light intensity of the light beam 111 is weakened, and two obvious sedimentation measurement signals are obtained as the test strip. Signal and quality control band signal (T and C in the figure). When the optical detection path OP starts to leave to the first window 211, when the light beam 111 is irradiated to the cassette 21 of the test strip 2, the measurement signal returns to near zero, and the entire detection process is completed.

In the foregoing detection procedure, the test piece moving device 4 can be used as an automatic drive device or a manual drive device. The detection speed of the test piece 2 advancement speed only affects the response time of the overall measurement signal, and the measurement signal of settlement ( T and C in the figure) are not affected by the advance speed, so they can be used as the basis for measurement and analysis.

The following will define characteristic parameters and values:

The first window time ΔA refers to the elapsed time from when the light beam 111 starts to enter the first window 211 and leaves the first window 211.

The background signal refers to the average value Vav of the measurement signal of the light beam 111 passing through the blank block 221 of the translucent test strip 22.

The test strip signal T refers to the lowest value of the measurement signal of the light beam 111 passing through the test strip T.

The quality control band signal C refers to the lowest value of the measurement signal of the light beam 111 passing through the quality control band C.

△ T = ABS│Background signal – Test strip signal│

△ C = ABS│Background Signal-Quality Control Band Signal│

Test band signal to noise ratio SNR (T) = △ T / background signal

Quality control with signal to noise ratio SNR (C) = △ C / background signal

Set the average value of the measurement signal Vav of the blank block 221 of the light-transmitting test strip 22 as the background signal, and calculate the sedimentation measurement signals of the test zone T and the quality control zone C (T and C in the figure) ) To test band signal T and quality control band signal C, normalize the difference between the test band signal T and quality control band signal C and the background signal (△ T, △ C) and the background signal, respectively. Obtain the test band signal noise ratio SNR (T) and quality control band signal noise ratio SNR (C).

As shown in Figure 4, the value of the measurement signal under this definition is between 0 and 1. When the light beam 111 has not entered the first window 211, the light beam 111 is irradiated on the cassette 21 of the test strip 2. The cassette 21 is shielded, and almost no light beam 111 enters the optical sensor 32, and the value of the measurement signal approaches 0; when the light beam 111 enters the first window 211 and shines on the blank block 221 on the test strip 22 A large amount of light beam 111 passes through the test strip 22 and enters the optical sensor 32. The measurement signal value increases sharply, so that the response curve rises from the zero point, and then when the quality control zone C and the test zone T pass the detection, respectively. During the path, because some of the light intensity is absorbed by the coloring material 222, two clear settlement signals can be seen respectively. After the detected beam 111 passes through the first window 211, the beam 111 is again blocked by the cassette body 21 and returns to zero. , Calculate the difference between the settlement signal of the quality control zone C and the test zone T and the background signal, respectively, to obtain the signal-to-noise ratio SNR (T) of the test zone and the signal-to-noise ratio SNR (C) of the quality control zone, respectively. As the concentration of the target to be detected in the test strip 2 increases, the value of the signal-to-noise ratio SNR will gradually increase as the density of the coloring material 222 in the test zone T and the quality control zone C increases.

The influence of different diameter widths of the first aperture 121 and the second aperture 311 in the optical detection path OP on the measurement signal is further explored, that is, a potential waveform diagram of the output measurement signal as shown in FIG. 5 is obtained. When the light source module 1 has a fixed wavelength and frequency of the light source 11 and the diameter of the first aperture 121 is fixed at 1.0 mm, the second aperture 311 has a protein concentration of 100 mIU on the hCG sample under the conditions of 0.1 mm and 0.3 mm, respectively. The test strip 2 is tested, and the first window time ΔA of the obtained potential waveform of the output measurement signal is normalized.

The following will introduce the normalization operation method in the first window time, and define the parameters and values with characteristic significance here:

First window time △ A = A1-A0

Time difference △ a ₁ = a ₁ -a ₀

Time difference △ a ₂ = a ₂ -a ₀

Time difference △ a _n = a _n -a ₀

Normalize the first window time = △ a _{1 … n} / △ A

As shown in FIG. 4, when the light beam 111 starts to enter the first window 211, the light beam 111 passes through the blank area 221 of the test strip 22, and the time point at which the optical sensor 32 starts to receive the measurement signal is set to the time axis zero point. A0, a _0, a first light beam 111 leaving the timeline window 211 is A1, A1-A0 is △ a, that is, the first time window. In the first time window, the time value of each measured signal are _{a 1, a 2 ... a n} , and a time axis zero value after subtracting a _0, a time difference is obtained for each measurement signal △ a ₁ , △ a ₂ … △ a _n . Finally, all the time difference values Δa ₁ , Δa ₂ … △ a _n are divided by the first window time ΔA, respectively, that is, the first window time ΔA of the complete measurement signal is normalized.

After the first window time ΔA of the measurement signal is normalized, when the diameter of the first aperture 121 is fixed at 1.0 mm, the measured signal potential waveform of the diameter of the second aperture 311 at 0.3 mm is higher than that of the second aperture 311 The measured signal potential waveform is 0.1mm in diameter. Therefore, in the optical detection system OD of this embodiment, when the diameter of the first aperture 121 is fixed and the light output condition is fixed, increasing the diameter of the second aperture 311 will cause the background signal and the measurement signal to increase at the same time. Further analysis of the test band signal-to-noise ratio SNR (T) of the two, as shown in Fig. 5, found that the test band signal-to-noise ratio SNR (T) curve of the second aperture 311 with a diameter of 0.1 mm is higher than that of the first band 311. The test signal-to-noise ratio SNR (T) curve of the two apertures 121 having a diameter of 0.3 mm.

In addition, as shown in FIG. 5, when the test strip moving device 4 uses the manual driving device to move and test the test strip 2, although the measurement length of the first window time ΔA of each measurement signal may be different, the amount of measurement After the measurement signals are normalized, the time points of the settling measurement signals of the respective test band signals T and the quality control band signals C are consistent. Therefore, the normalization of the first window time ΔA helps to simplify the subsequent measurement signal analysis process.

The optical detection system OD uses the combination of the first aperture 121 and the second aperture 311 to limit the width of the beam 111 irradiated on the test strip 2 and restrict the background signals outside the test zone T and quality control zone C from entering the optical sensing. The device 32 can prevent the blank block 221 of the test strip 22 and the measurement signals of the test strip T or the quality control band C from entering the optical sensor 32 at the same time, which causes the phenomenon that the background signal and the signal-to-noise ratio SNR rise.

FIG. 6 is a schematic diagram of the signal-to-noise ratio of the test strip of this embodiment. The test strip 2 with different protein concentrations of 0.25mIU and 100mIU of hCG samples cooperates with the first pore 121 and the second pore 311, and analyzes each test. With signal to noise ratio SNR (T) value changes. In this embodiment, when the diameter of the first aperture 121 is fixed at 0.1 mm, the test band signal-to-noise ratio SNR (T) value of the test strip with a diameter of 1.0 mm of the second aperture 311 is higher than the diameter of the second aperture 311 is 6.0. The test band signal noise ratio (mm) of the mm. When the diameter of the second aperture 311 is fixed at 6.0 mm, the signal-to-noise ratio SNR (T) value of the test strip with a diameter of 0.1 mm of the first aperture 121 is higher than that of the test strip with a diameter of 1.0 mm of the first aperture 121 Noise ratio SNR (T) value. It can be seen that no matter whether the diameter of the first aperture 121 or the second aperture 311 is fixed, reducing the diameter of another aperture can improve the detection signal-to-noise ratio SNR value, and through the first aperture 121 and the second aperture The cooperation of the aperture 311 can obtain a clear and easy-to-analyze measurement signal potential waveform and a better signal-to-noise ratio SNR value. Especially under the condition that the diameter of the first aperture 121 is reduced to 0.1 mm and the diameter of the second aperture 311 is reduced to 1.0 mm, the best signal-to-noise ratio SNR value can be obtained.

As shown in FIG. 6, by quantifying the signal-to-noise-to-noise ratio SNR value, the concentration of the target to be detected in the test strip 2 can be further calculated.

In summary, the optical detection system of the present invention measures the detection signal hidden inside the test strip fiber through a penetrating optical detection path to improve the intensity of the detection signal; the scanning design of the optical detection path is used to The long axis of the first window of the test piece sequentially scans the blank areas, test strips and quality control strips of the test strip in the first window in order to reduce the number of optical sensors and reduce the complexity of the design of the reading device. It also increases the flexibility of increasing and decreasing the number of test strips on the test strips, while reducing the precision requirements for drawing test strips and quality control strips.

The optical detection system of the present invention uses the first pore and the second pore in the penetrating optical detection path to cooperate with each other to increase the reliability of the measurement signal, improve the signal-to-noise ratio and strength of the measurement signal, and improve the measurement signal. The signal-to-noise ratio SNR value further reduces the minimum detection limit (LOD) of the test strip, thereby achieving the purpose of improving the convenience and sensitivity of the testing instrument.

1‧‧‧light emitting module

11‧‧‧ light source

111‧‧‧ Beam

12‧‧‧The first shading unit

121‧‧‧ first pore

2‧‧‧test strip

21‧‧‧ Cassette

211‧‧‧first window

212‧‧‧Second window

213‧‧‧ specimen opening

22‧‧‧ test strip

221‧‧‧Blank block

222‧‧‧Coloring material

3‧‧‧Receiving module

31‧‧‧Second shading unit

311‧‧‧second pore

32‧‧‧optical sensor

4‧‧‧ Test piece moving device

C‧‧‧Quality control zone, quality control zone signal

T‧‧‧test strip, test strip signal

MA ₂₁₁ ‧‧‧ Long axis direction

OD‧‧‧optical detection system

OP‧‧‧Optical detection path

SNR, SNR (21), SNR (221), SNR (T), SNR (C) ‧‧‧Signal to noise ratio

Vav‧‧‧Average

A0, A1, a ₀ , a ₁ , a ₂ , an _n ‧‧‧ time value

△ A‧‧‧First window time

△ a ₁ , △ a ₂ , △ a _n ‧‧‧ time difference

FIG. 1 is a schematic diagram of an optical detection system according to an embodiment of the present invention. FIG. 2 is a schematic diagram of an optical detection path according to an embodiment of the present invention. FIG. 3A is a schematic perspective view of a test strip according to an embodiment of the present invention. FIG. 3B is an exploded view of the test piece shown in FIG. 3A. FIG. 4 is a schematic diagram of a potential waveform of an output measurement signal according to an embodiment of the present invention. FIG. 5 is a schematic diagram of a potential waveform of an output measurement signal according to another embodiment of the present invention. FIG. 6 is a schematic diagram of a test strip signal-to-noise ratio according to an embodiment of the present invention.

Claims (13)

An optical detection system includes: a light-emitting module including: a light source for providing a light beam; and a first light-shielding unit having a first aperture, the first aperture is disposed corresponding to the light source; and a test strip includes : A cassette having a first window, a second window, and a specimen opening, the specimen opening is disposed on a surface of the cassette, and the first window is corresponding to the second window and is respectively opened in the On the opposite sides of the cassette, the first window is disposed corresponding to the first aperture; and a test strip is disposed in the cassette; and a receiving module includes: a second light shielding unit having a second An aperture, and the second aperture is disposed corresponding to the second window; and an optical sensor for receiving the light beam and emitting a measurement signal, wherein the light beam exits the first light shielding unit through the first aperture The light beam sequentially penetrates the first window, the test strip, and the second window and enters the second light shielding unit through the second aperture. The optical detection system according to item 1 of the patent application scope, wherein the test strip includes at least a test strip and a quality control strip, and the test strip and the quality control strip are distributed in the first window and the second window Within the intersection of the vertical projection planes on the test strip. The optical detection system according to item 2 of the patent application scope, wherein a diameter of the first aperture is smaller than or equal to a width of the test strip and a width of the quality control strip. The optical detection system according to item 1 of the application, wherein the diameter of the second aperture is smaller than or equal to the diameter of the first aperture. The optical detection system according to item 1 of the scope of patent application, wherein the diameter of the first aperture is between 0.1 and 5.0 mm. The optical detection system according to item 1 of the patent application scope, wherein the light source is a light-emitting diode, and the test strip further has a coloring material, and the wavelength of the light emitted by the light-emitting diode is the coloring material. Wavelength of absorbed light. The optical detection system according to item 1 of the scope of patent application, wherein the light source, the first aperture, the second aperture, and the optical sensor together form an optical detection path, and the optical detection path is substantially perpendicular to the detection test. sheet. The optical detection system according to item 1 of the patent application scope further includes: a test piece moving device for fixing and driving the test piece to move linearly along the long axis direction of the first window, so that the light beam moves along the A part of the cassette and the first window are irradiated to the long axis direction. The optical detection system according to item 8 of the patent application scope, wherein the test piece moving device is an automatic driving device or a manual driving device. The optical detection system according to item 9 of the patent application scope, wherein the automatic driving device includes a transmission device such as a slide rail, a screw, a gear or a belt, and is connected to a motor. The optical detection system according to item 9 of the scope of the patent application, wherein the manual driving device is designed with a slotted slider and a sliding slot, and the detection test piece is directly moved by a finger for linear motion. The optical detection system according to item 1 of the patent application scope further includes a signal analysis module, including: a signal analysis unit that receives the measurement signal and acquires parameters from the measurement signal, where the parameter is a A background signal parameter, a quality control signal parameter, a test signal parameter or a first window time parameter; and a signal calculation unit, which uses at least one of the parameters to perform calculations to calculate a specific substance concentration in the test object. The optical detection system according to item 1 of the scope of patent application, measures a detection signal hidden inside the fiber of the test strip through a penetrating optical detection path.