201121580 六、發明說明： 【發明所屬之技術領域】 是一種女性私密處清潔劑及 本發明係有關於一種清潔劑，特別 其製造方法。 【先前技術】 近年來於微生物學上的研究結果顯示，在人體的一些巧官 中，例如腸道或陰道等，存在有數十萬億且多達12〇種以上的微 生物。其中’如雙歧㈣和乳_等對人體有益的厭氧菌大約佔 有95%，而如大腸桿菌等對人體有害的厭氧菌僅佔有約以左右。 這些厭氧菌在器官内係相互制約並互相依存，進而使器官内維持 微生物動力生«統的平衡，使人體保持健康㈣。因此，當這 些器官内的微生物動力生财'統遭受破壞時驗使器官受到病 菌的感染而產生疾病。 以女性陰道炎為例。在健康姐的陰勒皆具有-絲量的 1菌、’处減_赠道簡所分_糖原做為能量來源’ 、殖並疋殖於陰相。並且，這魏酸时在陰勒與其他病 原菌競爭定魏域耐顧勢_，藉叩讀其他病於陰道 内增殖，而轉陰道⑽清潔度。細，當健康女性在衛生環境 生物二力:降時’則容易使陰道遭受細菌、黴菌或滴蟲等微 物的感朱，使乳酸菌受到這些病原菌的攻擊 陰道内微錄動力钱㈣醉衡，料致姐陰敍的發生 一般在女性陰道炎的治療上，大多是施以續胺、抗生素或其 201121580 他消毒樂物進行治療。‘然而，這種治療方式雖然可以達到纖滅病 原_絲’但㈣軸續方式並料有_糊性，因此在 殲滅病原菌的同時’會—併的抑制乳酸菌於陰道内的增殖，進而 造成陰道_微生物動力生‘㈣統無法獲得平衡，致使乳酸菌對 其他病原菌的制約作用喪失。同時，奸期的使料胺、抗生素 或其他消毒藥物進行治療，容易使病原菌產生抗藥性而使治療效 果降低。並且’對於孕婦而言亦不適用這種治療方式，藉以避免 #干擾胎兒的正常生長與發育。因此，傳統上對女性陰道炎的治療 方式在使用上’容易因上述因素的影響而受到限制。 /目前在女性陰道炎的治療上，已發展出—種微生物治療法， 其係將處理後之乳酸gg體直接的置人於陰勒，使乳酸菌於陰 道内形成優勢族群，以達到制約其他病原菌於陰道内增殖的效 果。然而現有之财物治絲槪對场桿__祕⑽幻、 綠腹私菌、金黃色葡萄球菌 •啊咖績啦鏈球菌屬㈣咖⑽)等細菌產生抑制 作用。但疋對於白色念珠菌ϋα财)及陰道加德納菌 (G^r办並無法產生拮抗作用。並且，在這種微生物 治療法中，其乳酸菌需經過長達36至72小時的發酵培養後才能 獲得所需之乳量’以便於再進行後續的加玉處理後方能使 用。因此在製造上將承受相當大的時間及成本壓力。 【發明内容】 鑒於以上的問題，本發明提供一種女性私密處清潔劑藉以改 201121580 良習知使料胺、抗生素或其他消毒藥物治療姐陰道炎時，容 易使病產生抗藥性，以及不翻於孕婦的問題。並藉以改良 目前在微生物治療上，無法對白Μ補造成拮抗作用以及製程 時間過長所導致成本增加的問題。 本發明揭露-種姐私密處清麵之製造方法，包括有下列步 驟：首先’接種至少-乳酸_株至—培養液中，以形成一乳酸 菌培養液。接著’將乳_培養液培養於贼至机的環境中， 並且加入-發酵促進劑於該乳酸菌培養液中，使發酵促進劑與乳 酸菌菌株行-生化反應。然後，將乳_培養液自耽至贼的 環境中取出，並對乳_培養液執行—離心操作，藉以得到一乳 酸菌發酵液及-紐。接著，收集乳義發酵液其具有一過氧 化氫及—觸素，而完成女雖密處清細的製備。 本發明並揭露-種姐私贿清雜，錢由上述之女性私密 處清潔劑之製造方法所製備而成。此姚私密錯軸包括一乳酸 菌發酵液，其具有一過氧化氫及一抑菌素。 本發明所麟之姐减處清細之製造方法，魏酸菌培養 液的培養過程中，添加發酵促進劑於乳酸菌培養液中，以藉由發 酵促進劑魏酸gg株行生化反應，並提供__株於繁瘦增 長時所需之生長能量。因此，可增加乳酸_株於⑽菌培養液 中的含量/濃度，並大幅縮短乳酸菌培養液的培養時間進而節省 女性私密處清潔劑的製備時間及成本。 以上之關於本發明内容之說明及以下之實施方式之說明係用 201121580 以不犯與轉本㈣之原理，並且提供本發明之專财請範圍更 進一步之解釋。 【實施方式】 請參閱「帛1圖」’本發明_實關賴露之姐私密處清潔 劑係應用於女性陰道炎之防治。在姐私密處清潔泰製造上，首 先將至少一乳酸菌菌株(strain)接種於一培養液中，使形成一乳酸 菌培養液(S101)。其中，可選用之乳酸菌菌株係選自於乾酿乳桿菌 • 仏⑽—d/k⑽叻、副乾酪乳桿菌⑹Ctokd//卿㈣c_)、乾路 乳才干鹵鼠李糖亞種(Ζύ[伽^•細⑽以· Sp似⑽卿⑽)、嗜酸性乳酸 得菌（Lactobacillus acidophilus)、比菲德氏溘（Lactobacillus 6)、短乳酸桿菌·"⑽&rm·5)、保加利亞乳酸桿菌 (iMetobaeiUus bulgaricus) ' 龜維二糖乳桿镜（Lact〇bacUlus cellobiosus)、溶路乳桿镜(Lactobacillus collinoides)、戴白氏乳酸桿 菌（Lactobacillus delbrueckii)、發酵乳桿菌（LactobaciUus 鲁 、詹氏乳酸桿菌_/e?wem7)、加氏乳酸桿菌 (Lactobacillus gasseri)、植物乳後桿菌(LactobaciHusplantamm)、雷 曼氏乳酸桿菌rAamnosws)、纖維二糖乳桿菌 (Lactobacillus cellobiosus)、捲縮乳酸桿菌（lactobaciHus 〇*吻⑽加）、洛德乳桿菌(ZflctokczV/Ms reMim·)或德布律克乳酸桿菌 « (Lflcto^adZ/ws 等菌株’或上述菌株之其混合物。而可 選用之培養液為MRS培養基、乳清、奶類及殿粉其中之一，或上 述之混合物。其中，奶類可包括··濃縮牛奶、脫脂牛奶及低脂牛 201121580 奶等。 於本實施财，伽x祕乳㈣、域乳酸桿“洛德 菌接種於雜MRS培養基中’⑽魏_培麵做為舉例= 明，但並不以此為限。201121580 VI. Description of the invention: [Technical field to which the invention pertains] is a female intimate cleansing agent and the present invention relates to a cleaning agent, particularly a method of manufacturing the same. [Prior Art] In recent years, microbiological studies have revealed that there are tens of trillions and up to 12 or more types of microorganisms in some human body, such as the intestines or the vagina. Among them, anaerobic bacteria such as bifid (four) and milk _ are beneficial to the human body, accounting for about 95%, while anaerobic bacteria such as Escherichia coli which are harmful to human body only occupy about or so. These anaerobic bacteria are mutually constrained and interdependent in the organs, thereby maintaining the balance of microbial power in the organs and keeping the human body healthy (4). Therefore, when the microbial powers in these organs are destroyed, the organs are infected by the bacteria to cause diseases. Take female vaginitis as an example. In the health sister's cockroach, there is a silky amount of 1 bacteria, 'minus _ gift simplification _ glycogen as a source of energy', colonization and colonization in the Yin phase. Moreover, this Wei acid competes with other pathogens in the Wei-Ding to determine the Wei domain resistance _, by reading other diseases in the vaginal proliferation, and turning the vagina (10) cleanliness. Fine, when a healthy woman is in a hygienic environment, the second thing: when it is lowered, it is easy for the vagina to suffer from the micro-objects such as bacteria, mold or trichomoniasis, so that the lactic acid bacteria are attacked by these pathogens and the micro-recorded power in the vagina (4) drunk, It is expected that the occurrence of female vaginal gynecology is generally treated in female vaginitis, and most of them are treated with a re-amine, antibiotic or its 201121580 disinfectant. 'However, although this treatment method can achieve the fiber-killing pathogen _ silk', but (4) the continuation method has the _------------------------------------------------------------------------------------------------------------------------------------------------- _ Microbial power generation '(4) system can not get balance, resulting in the loss of lactic acid bacteria to other pathogens. At the same time, the treatment of amines, antibiotics or other disinfecting drugs in the rape period tends to make the pathogens resistant and the therapeutic effect is reduced. And 'this treatment is not applicable to pregnant women to avoid interference with the normal growth and development of the fetus. Therefore, the traditional treatment of female vaginitis is limited in its use by the influence of the above factors. / At present, in the treatment of female vaginitis, a microbial treatment method has been developed, which directly treats the lactic acid gg body in the yin, so that the lactic acid bacteria form an advantageous group in the vagina to restrict other pathogenic bacteria. The effect of proliferation in the vagina. However, the existing property control silkworms have inhibitory effects on bacteria such as __ secret (10) illusion, green-bellied bacterium, Staphylococcus aureus, ah, and genus Streptococcus (4) coffee (10). However, 疋 does not produce antagonism for Candida albicans and G. vaginalis (G^r), and in this microbial treatment, the lactic acid bacteria need to undergo fermentation for up to 36 to 72 hours. In order to obtain the required amount of milk', it can be used after the subsequent jade treatment. Therefore, it will bear considerable time and cost pressure in manufacturing. SUMMARY OF THE INVENTION In view of the above problems, the present invention provides a female privacy. When the cleaning agent is used to change the 201121580, it is easy to make the disease resistant to the disease and not to the pregnant woman when it is used to treat the vaginal inflammation. The invention relates to a method for manufacturing an antagonism and an increase in cost caused by an excessively long process time. The invention discloses a method for manufacturing a clear face of a sister, comprising the steps of: first inoculating at least a lactic acid to a culture solution, Forming a lactic acid bacteria culture solution. Then, the milk-culture solution is cultured in a thief-to-machine environment, and a fermentation promoter is added to the milk. In the culture medium, the fermentation promoter and the lactic acid bacteria strain are subjected to biochemical reaction. Then, the milk_culture liquid is taken out from the environment of the thief, and the milk-culture liquid is subjected to centrifugation operation to obtain a lactic acid bacteria fermentation liquid. And - New Zealand. Next, the milky fermentation broth has a hydrogen peroxide and a chemo-activator, and the preparation of the female secret is fine. The invention also discloses that the sister is privately bribed and the money is made by the above female The preparation method of the cleaning agent in the privacy part comprises a lactic acid bacteria fermentation liquid, which has a hydrogen peroxide and a bacteriocin. The manufacturing method of the singer of the invention is reduced. During the cultivation of the acid bacteria culture solution, a fermentation promoter is added to the lactic acid bacteria culture solution to carry out a biochemical reaction by the fermentation promoter weiqi gg, and provides the growth energy required for the growth of the __ strain. The content/concentration of the lactic acid _ strain in the (10) broth can be increased, and the culture time of the lactic acid bacteria culture solution can be greatly shortened, thereby saving the preparation time and cost of the female private cleaning agent. The description and the following description of the embodiments are made with 201121580, and the explanation of the scope of the present invention is provided without further explanation. [Embodiment] Please refer to "帛1图" "The invention" Guan Lai's sister intimate cleansing agent is applied to the prevention and treatment of female vaginitis. In the intimate cleansing of Thai manufacturing, at least one strain of lactic acid bacteria is first inoculated into a culture solution to form a lactic acid bacteria culture solution ( S101), wherein the selected lactic acid bacteria strain is selected from the group consisting of Lactobacillus sinensis • 仏(10)-d/k(10) 叻, Lactobacillus paracasei (6) Ctokd//Qing (4) c_), dry road milk, and rare rhamnose subspecies (Ζύ [Gamma•fine (10) to · Sp like (10) Qing (10)), Lactobacillus acidophilus, Lactobacillus 6, Lactobacillus sp., "10&rm·5), Bulgarian lactate Bacillus (iMetobaeiUus bulgaricus) 'Lact〇bacUlus cellobiosus, Lactobacillus collinoides, Lactobacillus delbrueckii, Lactobacillus fermentum Uus 鲁, Lactobacillus japonicus _/e?wem7), Lactobacillus gasseri, Lactobaci Husplantamm, Lactobacillus r'amnosws, Lactobacillus cellobiosus, Roll Lactobacillus lactis (lactobaci Hus 〇 * kiss (10) plus), Lactobacillus laurel (Zflctokcz V / Ms reMim ·) or Debrokes Lactobacillus « (Lflcto ^ adZ / ws and other strains ' or a mixture of the above strains. The culture medium which can be used is one of MRS medium, whey, milk and house powder, or a mixture as described above. Among them, milk can include · · concentrated milk, skimmed milk and low-fat cattle 201121580 milk. In this implementation, the gamma x secret milk (4), the domain lactic acid rod "Drodia inoculation in the mixed MRS medium" (10) Wei _ Pei surface as an example = Ming, but not limited to this.
接著，將乳酸菌培養液放置於如艺至4〇t的環境中培養U 至20小時’例如培養16至18小時。此培養時間的長短翁= 乳酸菌_於培養液巾㈣輯度而定。並且，在魏菌培養液 放置於上狀培養環境之前歧於乳_培她於上述培養環境 中進行培養時’加人-發酵促_於乳_培養液巾。例如選^ 如幾丁質及寡醣(如木寡醣(Xyl0〇lig〇saccharide))等發酵促進劑，但 並不以此為限。同時，在乳酸菌培養液的培養過程中可進一步 地對乳_培養賴行攪拌操作，藉以使發酵促進舰均句混合 於乳酸菌培液中(S102)。 0 因此，當乳酸菌培養液中的乳酸菌菌株與發酵促進劑行生化 反應後，乳酸菌菌株能充分的利用發酵促進劑所提供的生長能 量，而加速其於乳酸菌培養液中的增殖速度(或濃度），進而有效縮 短乳酸菌培養液的培養時間。 此外，如「第2圖」所示，在本發明之另一實施例中，將含 有發醱促進劑之乳酸菌培養液培養於⑽艽至4〇〇c的環境時，可一 併地通入二氧化碳(C〇2)於乳酸菌培養液中(S2〇2)，以藉由乳酸菌 菌株之厭氧特性，而加快乳酸菌菌株的增殖速度。因此，經由通 入二氧化碳於乳酸菌培養液之步驟，亦可有效的縮短乳酸菌培養 201121580 液的培養時間。 、請再次參閱「第1圖」，當乳酸菌培養液中之乳酸菌菌株濃度 達到預設之濃度後’魏_培養缝聰至贼的環境中: 出°之後’將乳酸菌培養液置於坨至邮的低溫環境下進行離 心操作。·藉_，續在的環境下，骑分鐘4_轉的轉 速(4___跑_培離心15分鐘。使乳_培養液中 的菌體魏_發酵_上清液(亭__分離。因此，於離 心操作完成後’可得到乳酸菌發酵液及菌體(sl〇3)。 接著，收集乳酸菌發酵液(S104)，此乳酸菌發酵液中包含過氧 化虱(h202)及抑菌素等成分。並且，可視使用上之需求而將菌體保 存備用或丢棄。然後’過魏gu發酵液(S1G5)，其係糊一分子 篩網對乳義發酵液進行過遽，藉赠、除乳㈣發酵液中之其它 雜質。使乳_發中近乎於僅具有過氧化纽_素等成分。 於乳酸菌發酵液過濾後，可選擇性的加入界面活性劑於乳酸 菌發酵液中，以完成女性私密處清潔劑的製備。此女性私密處清潔 劑並具有介於3.5至4.5之間的酸鹼值(pH value)，例如為具有4 〇 之酸鹼值。其中，可使用的界面活性劑為兩性離子界面活性劑、 非離子界面活性劑或陰離子界面活性劑，如月桂醚硫酸鈉(SodiumNext, the lactic acid bacteria culture solution is placed in an environment of, for example, 4 Torr to culture for U to 20 hours', for example, for 16 to 18 hours. The length of the culture period = lactic acid bacteria _ in the culture liquid towel (four). Further, before the culture solution of the bacterium is placed in the upper culture environment, the milk is cultured in the above culture environment, and the human-fermentation is promoted. For example, fermentation promoters such as chitin and oligosaccharides (such as Xyl0〇lig〇saccharide) are selected, but are not limited thereto. At the same time, in the culturing process of the lactic acid bacteria culture solution, the milk-culture can be further stirred, whereby the fermentation-promoting ship is mixed with the lactic acid bacteria culture solution (S102). 0 Therefore, when the lactic acid bacteria strain in the lactic acid bacteria culture solution and the fermentation accelerator biochemical reaction, the lactic acid bacteria strain can fully utilize the growth energy provided by the fermentation accelerator to accelerate the proliferation rate (or concentration) in the lactic acid bacteria culture solution. Further, the cultivation time of the lactic acid bacteria culture solution is effectively shortened. Further, as shown in the "Fig. 2", in another embodiment of the present invention, the lactic acid bacteria culture solution containing the hairpin promoter is cultured in an environment of (10) 艽 to 4 〇〇c, and can be uniformly introduced. Carbon dioxide (C〇2) is in the lactic acid bacteria culture solution (S2〇2) to accelerate the proliferation rate of the lactic acid bacteria strain by the anaerobic property of the lactic acid bacteria strain. Therefore, the culture time of the lactic acid bacteria culture 201121580 can be effectively shortened by the step of introducing carbon dioxide into the lactic acid bacteria culture solution. Please refer to "Picture 1" again. When the concentration of the lactic acid bacteria strain in the lactic acid bacteria culture solution reaches the preset concentration, 'Wei_Cultivate the seam to the thief's environment: After the °', the lactic acid bacteria culture solution is placed in the 坨 坨Centrifugal operation in a low temperature environment. · Borrow _, continue in the environment, ride the minute 4_ turn of the speed (4___ run _ culture centrifugation for 15 minutes. Make the bacteria in the milk _ broth Wei _ fermentation _ supernatant (King __ separation. Therefore After the completion of the centrifugation operation, the lactic acid bacteria fermentation liquid and the bacterial cells (sl 3) are obtained. Next, the lactic acid bacteria fermentation liquid (S104) containing the components of cerium peroxide (h202) and bacteriocin is collected. In addition, the cells can be stored or discarded according to the needs of the use. Then, the Weigu fermentation broth (S1G5) is used to filter the milky fermentation broth, lending, defrosting (4) fermentation. Other impurities in the liquid. Make the milk _ hair almost only have components such as oxidized ketone. After filtering the lactic acid bacteria fermentation broth, the surfactant can be selectively added to the lactic acid bacteria fermentation broth to complete the cleaning of the female private place. Preparation of the agent. This female intimate detergent has a pH value of between 3.5 and 4.5, for example, having a pH of 4 。. Among them, the surfactant can be used as a zwitterionic interface. Active agent, nonionic surfactant or anionic interface activity , Such as sodium lauryl ether sulfate (Sodium
Laureth Sulfate，簡稱 SLS)及十二烷基硫酸銨(Ammonium laUiyl sulfate ’簡稱ALS)等。此外’於加入界面活性劑的同時，可選擇 性的加入如醋酸、擰檬酸或乳酸等酸性成分於乳酸菌發酵液中 (S106) ’藉以使女性私密處清潔劑能常態地維持介於3 5至4 5之 9 201121580 間的酸鹼值，並增加乳酸菌發酵液的抑菌效果。 之後’對本貫把例之女性私密處清潔劑的抑菌能力進行測試， 用以測試之菌種分別為白色念珠菌、乙型鏈球菌(伽职卿· aga/actiae)、大腸桿菌(E co/i)、陰道加德納菌、克雷白氏肺炎菌 (Klebsiella pneumoniae)反奇異雙型椁镆(pr〇teus mimbu的専。首 先，將女性私密處清潔劑分裝至複數支試管中，使每一支試管具 有500μ1(微升)的女性私密處清潔劑。然後，取25μ1上述菌種之菌 液分別添加至試管中，並使菌液與女性私密處清潔劑混合，分別 加入2_5 ml之細菌培養液，最後加入無菌生理食鹽水使每支試管 之液體體積為5 ml。其中’上述菌種製備為從血液瓊脂平板(B1〇〇d Agar Plate)挑取培養18_24小時之菌種菌落至必帝鑑別培養液(bd Phoenix ID Broth)，調至相當於MacFarland 〇 5之標準濁度懸浮 液’其菌液濃度相當於UdyCFU/mi，即每毫升具有丨5χΐ〇8之 痛落形成單位(colony f〇rming units，CTU)。 接著，將混合有菌液之試管置於二氧化碳培養箱中，於35¾ 的環境下培養18_24小時後’將試管從二氧化碳培養箱中取出，並 觀察上述龍的生長狀況，其結果如下表一所示。 表一 檢測結果 無生長 無生長 抑菌率 100% 100% 菌種名稱 白色念珠菌 -----— 乙型鍵球菌 10 201121580 大腸桿菌 陰道加德納菌 克雷白氏肺炎菌 -----____ 奇異變型桿菌Laureth Sulfate (SLS) and Ammonium laUiyl sulfate (ALS). In addition, while adding the surfactant, it is possible to selectively add an acidic component such as acetic acid, citric acid or lactic acid to the lactic acid bacteria fermentation broth (S106), so that the female privacy agent can be normally maintained at 3 5 Between 4 5 and 9 201121580, the pH value increases the bacteriostatic effect of the lactic acid bacteria fermentation broth. After that, 'the antibacterial ability of the female intimate cleansing agent was tested. The strains tested were Candida albicans, Streptococcus B (Glyph of aga/actiae), and E. coli (E co /i), Gardnerella vaginalis, Klebsiella pneumoniae anti-singular bitype 椁镆 (pr〇teus mimbu 専. First, the female private cleaning agent is dispensed into a plurality of test tubes, Make each tube with 500μ1 (microliter) of female intimate cleansing agent. Then, add 25μ1 of the above bacterial strain to the test tube, and mix the bacterial liquid with the female private cleansing agent, add 2_5 ml respectively. The bacterial culture solution is finally added to the sterile physiological saline solution so that the liquid volume of each test tube is 5 ml. The above-mentioned strains are prepared by picking up the bacterial colonies from the blood agar plate (B1〇〇d Agar Plate) for 18-24 hours. Bd Phoenix ID Broth, adjusted to the standard turbidity suspension of MacFarland 〇5', the concentration of the bacterium is equivalent to UdyCFU/mi, that is, the pain-forming unit with 丨5χΐ〇8 per ml (colony f〇rming units Then, the test tube mixed with the bacterial liquid was placed in a carbon dioxide incubator, and after culturing for 18 to 24 hours in an environment of 353⁄4, the test tube was taken out from the carbon dioxide incubator, and the growth state of the above dragon was observed, and the results were as follows. Table 1 shows the results of the test No growth, no growth inhibition rate 100% 100% strain name Candida albicans ----- B betaella 10 201121580 Escherichia coli vaginal bacteria Kreb's pneumonia Bacteria-----____ singular bacillus
__________ /〇 珠菌、 魄糊及奇異__________ /〇 珠, paste and singular
^ ’如「第2圖」所示’在本發明之另—實施例中，為了 進一步增加女性私密處清潔劑的殺力，更可於界面活性劑加入 於乳酸菌發酵液時，額外的通人過氧化氫於乳_發酵液中 (S206) ’使製備完成後之女性私密處清潔劑具有較多的過氧化氣含 量X達到較佳的殺菌效能。此外，亦可以於乳酸菌發酵液中加 入券醣類(如木养醣㈣〇〇llg〇saccharide))添加物，以增力口陰道内原 生乳酸菌之活性。 在應用上，可將本發明之一實施例所揭露之女性私密處清潔劑 與-賦形劑進行結合，藉以讓女跡密處清麵能具有不同的使用 形態。例如，可將女性私密處清潔劑與水溶性高分子聚合物、高分 子多糖聚合物、水、食鹽水、凡士林油、甘油酯、甘油等賦形劑相 互結合’使形成液體'噴霧、凝膠、膠囊或乳液形態的女性私密處 清潔劑。另’可添加三仙膠(Xanthan Gum)、羧曱基纖維素 (carboxymethyl cellulose，CMC)、瓜爾膠(Hydroxypropyl Guar， HM21)等膠體之混合組成或其中之一，可以有效延長乳酸菌發酵 11 201121580 液停留在皮膚或陰道黏膜内的時間，以加強抑菌效果。此外，亦 可添加鼠尾草萃取液(Sage Extract)、洋甘菊萃取液(cham〇mile XtraCt)金盖I萃取液(Calendula Extract)或百里香紛(thymol)等天 然植物精油之混合組成或其中之一，以增加乳酸菌發酵液之抑菌 效果。並可將結合有女性私密處清潔劑之賦形劑進一步的配置於一 載體上，例如將液體、喷霧、凝膠、乾粉、或乳液形態之女性私密 處清潔劑配置於衛生棉、衛生龜、賴巾絲險套等載體上， 使女性私密處清潔劑可廣泛的應用於各種* _使用需求。 其中’以凝膠形態的女性私密處清潔劑為例，其將女性私密處 /月/承劑包覆於水溶性高分子聚合物所組成之凝膠中以做為外塗 劑或塞劑使用。在本實施例中，係以凝膠形態的女性私密處清潔劑 為塞劑做為舉例，但並不以此為I其巾，水溶性高分子聚 。物是由-液態的聚乙二醇或聚乙婦醇以及—固態的聚乙二醇、 聚乙稀醇、紐基纖維素或澱粉所組成，例如以液態之聚乙二醇 以及固態之羧酸基纖維素所組成之高分子聚合物。 然後’將此以凝膠形態的女性私密處清潔劑置入於遭受如大腸 桿菌、綠腹_、金黃色葡萄球菌、鏈球_、陰道桿狀桿菌及 白色念珠菌等病原菌感染之陰道内。由於陰道在遭受病原菌感染 時’其酸驗值會大於4.5，而不適於乳酸菌的生長。因此使乳酸菌 於陰道内成為弱勢鱗，進而造成陰道㈣發生。觀，當凝膠 形態的女性私密處清潔劑使用於陰道炎的處理時，首先，高分子聚 合物可有效延長此女性私密處清潔劑停留在陰道枯膜内的^刀間，加 12 201121580 強抑菌效果。此時，除了可藉由女性私密處清潔劑所具有的抑菌素 p制病原菌的增殖外，並可透過過氧化氣纖滅病原菌，進而減少 陰道内之大腸桿菌、綠膿桿菌、金黃色葡萄補、鏈球菌屬、陰 道桿狀桿菌及白色念珠菌等病原菌的數量。 其次’經由女性私密處_射界面活_進—步提升抑菌素 對病原_抑舰力。最後，由料跡密處清_賴驗值介於 =至45之間’因此可提供存在於陰勒之紐g較佳的生長環 _ ^。並伴縣抑g素及顿化氫的交互作用，使陰勒的乳酸菌 二在短時間之内重新的回復為優勢族群的狀態，進而使陰道内的 核境恢復為健康狀態，從而解決陰道炎的問題。 本發明所揭露之女性私密處清潔劑及其製造方法，藉由在乳酸 菌培養液的培養過程巾進行攪拌並添加發酵促_，啊選擇性 的通入二氧化碳營造厭氧環境’可有效增純酸菌菌株於乳酸菌 培養液内的濃度’並縮短乳酸菌培養液的培養時間。因此，可大 >幅的節省女性私密處清潔劑的製造、生產時間。並且，於乳酸菌發 酵液中加入界面活性劑並通入過氧化氫，使女性私密處清潔劑可同 時具有良好的抑菌及殺菌能力。 雖然本發明之實施例揭露如上所述，然並非用以限定本發 明’任何熟習相關技藝者’在不脫離本發明之精神和範圍内，舉 凡依本發明申請範圍所述之形狀、構造、特徵及精神當可做些許 之變更，因此本發明之專利保護範圍須視本說明書所附之申請專 利範圍所界定者為準。 13 201121580 【圖式簡單說明】 第1圖為本發明之一實施例之製造流程示意圖；以及 第2圖為本發明之另一實施例之製造流程示意圖。 【主要元件符號說明】 無^ 'As shown in the "Fig. 2", in another embodiment of the present invention, in order to further increase the killing power of the female private cleansing agent, it is also possible to add an additional surfactant when the surfactant is added to the lactic acid bacteria fermentation broth. Hydrogen peroxide in the milk_fermentation liquid (S206) 'The preparation of the female intimate cleansing agent after the preparation has more peroxidation gas content X to achieve better sterilization efficiency. In addition, it is also possible to add a saccharide (e.g., xylogan) to the lactic acid bacteria fermentation broth to enhance the activity of the original lactic acid bacteria in the vagina. In use, the female intimate cleansing agent disclosed in one embodiment of the present invention can be combined with an excipient so that the clear side can have different use forms. For example, a female intimate cleansing agent can be combined with an excipient such as a water-soluble high molecular polymer, a high molecular polysaccharide polymer, water, a saline solution, a petroleum jelly, a glycerin ester, or a glycerin to form a liquid spray or gel. Female intimate cleansers in the form of capsules or lotions. Another 'addition of Xanthan Gum, carboxymethyl cellulose (CMC), guar gum (Hydropropylpropyl Guar, HM21) and other colloids or one of them can effectively prolong the fermentation of lactic acid bacteria 11 201121580 The time the liquid stays in the skin or vaginal mucosa to enhance the antibacterial effect. In addition, one or a combination of natural plant essential oils such as Sage Extract, cham〇mile XtraCt, Calendula Extract or thymol may be added. In order to increase the bacteriostatic effect of the lactic acid bacteria fermentation broth. The excipient combined with the female intimate detergent may be further disposed on a carrier, for example, a liquid, spray, gel, dry powder, or emulsion form female intimate cleaning agent is disposed on the sanitary napkin, the sanitary turtle On the carrier, such as Lai towel silk cover, the female intimate cleaning agent can be widely used in various * _ use requirements. In the case of a female intimate cleansing agent in the form of a gel, a female intimate/month/loading agent is coated in a gel composed of a water-soluble polymer to be used as an external coating or a suppository. . In the present embodiment, a female intimate cleansing agent in the form of a gel is used as a stopper, but it is not used as a towel, and a water-soluble polymer is polymerized. The composition is composed of -liquid polyethylene glycol or polyethyl alcohol and solid polyethylene glycol, polyethylene glycol, neoall cellulose or starch, for example, liquid polyethylene glycol and solid carboxylate A high molecular polymer composed of acid-based cellulose. Then, the female intimate cleansing agent in a gel form is placed in the vagina infected with pathogenic bacteria such as Escherichia coli, Green abdomen, Staphylococcus aureus, Hammer bacillus, Corynebacterium vaginalis and Candida albicans. Since the vagina is infected with a pathogen, its acidity value will be greater than 4.5, which is not suitable for the growth of lactic acid bacteria. Therefore, the lactic acid bacteria become weak scales in the vagina, which causes the vagina (4) to occur. When the gel-formed female intimate cleansing agent is used in the treatment of vaginitis, first of all, the high molecular polymer can effectively prolong the female intimate place of the cleaning agent staying in the vaginal film, plus 12 201121580 strong Antibacterial effect. At this time, in addition to the proliferation of pathogenic bacteria caused by the antibacterial p which is possessed by the female intimate cleansing agent, the pathogen can be eliminated by the peroxidation gas, thereby reducing the Escherichia coli, Pseudomonas aeruginosa, and golden yellow grapes in the vagina. The number of pathogens such as complement, Streptococcus, Corynebacterium vaginalis and Candida albicans. Secondly, through the female private office _ shooting interface live _ advance - step to enhance the antibacterial against the pathogen _ suppression. Finally, it is clear that the trace value is between = and 45', so that a preferred growth ring _ ^ present in the yoke is provided. And with the interaction between the county and the hydrogen, the lactic acid bacteria 2 in the genus reverts back to the state of the dominant group in a short period of time, thereby restoring the nuclear environment in the vagina to a healthy state, thereby solving the vaginitis. The problem. The female intimate cleansing agent disclosed in the invention and the manufacturing method thereof can effectively increase the acid by stirring and adding the fermentation in the culture process of the lactic acid bacteria culture solution, and selectively introducing carbon dioxide into the anaerobic environment. The concentration of the strain in the lactic acid bacteria culture solution 'and shorten the culture time of the lactic acid bacteria culture solution. Therefore, it is possible to save the manufacturing and production time of the detergent in the female private place. In addition, a surfactant is added to the lactic acid bacteria fermentation broth and hydrogen peroxide is introduced, so that the female privacy agent can have good antibacterial and bactericidal ability at the same time. Although the embodiments of the present invention are disclosed as described above, it is not intended to limit the invention to any of the skilled in the art, and the shapes, structures, and features described in the scope of the present application are not departing from the spirit and scope of the present invention. And the spirit of the invention is subject to change. Therefore, the scope of patent protection of the present invention is subject to the scope of the patent application attached to the specification. 13 201121580 [Simplified description of the drawings] Fig. 1 is a schematic view showing a manufacturing process of an embodiment of the present invention; and Fig. 2 is a schematic view showing a manufacturing process of another embodiment of the present invention. [Main component symbol description] None
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