SU95262A1 - The method of cleaning technical insulin - Google Patents
The method of cleaning technical insulinInfo
- Publication number
- SU95262A1 SU95262A1 SU446446A SU445445A SU95262A1 SU 95262 A1 SU95262 A1 SU 95262A1 SU 446446 A SU446446 A SU 446446A SU 445445 A SU445445 A SU 445445A SU 95262 A1 SU95262 A1 SU 95262A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- insulin
- cleaning technical
- color
- precipitate
- Prior art date
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- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
Изобретение от оситс к способам очистки технического инсулина посредством осаждени балластных BeniecTB И1.елочью из подкисленного раствора, предварительно очищеиного изоэлектрическим осаждением аморфного инсулина, и применени последуюи ей его перекристаллизации .The invention is from Osit to methods for purifying technical insulin by precipitating ballast BeniecTB I1. From an acidified solution previously cleaned with isoelectric precipitation of amorphous insulin, and applying its subsequent recrystallization.
Преимуп,ествопредлагаемогоThe advantage offered by
способа перед известными заключаетс в том, что он устран ет потери ипсулииа при операции осаждени балластных веществ и позвол ет повысить активность получае .roro инсу.тииа.The method before the known one is that it eliminates the loss of ipsulia during the deposition of the ballast substances and makes it possible to increase the activity of obtaining .roro insu.tiya.
Предлагаемый способ состоит в том, что. с иелью определени окончани реакции осаждени балластных веи1.естз, окраску раствора, наход щегос над осадком, сравнивают с окраской 0,001 нормального раствора иода в растворе йодистого кали .The proposed method is that. With the determination of the end of the precipitation reaction of ballast veins, the color of the solution above the precipitate is compared with the color of a 0.001 normal solution of iodine in a solution of potassium iodide.
Способ осуи1ествл ют следующим образом.The method is described as follows.
Осадок аморфного инсулина после двух-трехкратного изоэлектрическог-о переос-аждени раствор ют в подкисленной сол ной кислотой воде. Полученный раствор разбавл ют водоп и добавл ют 30% ацетона , затем поди елачивают 57о-ным раствором едкого натра до тех пор, пока раствор инсулина примет окраску 0.001 нормального раствора иода в растворе йодистого кали . При Э1ИХ услови х выпадает окраиюнный содержащи до 10% инсулина осадок, которьп обрабатывают двукратно Казапным способом, а из полученного раствора 1П{сулпп осаждают изоэлектрическим способом .The precipitate of amorphous insulin after two to three times isoelectric interconversion is dissolved in water acidified with hydrochloric acid. The resulting solution is diluted with water and 30% acetone is added, then it is quenched with a 57 ° caustic soda solution until the insulin solution takes on a color of 0.001 normal iodine solution in a potassium iodide solution. Under EIICH conditions, a marginal up to 10% of insulin precipitates, which is treated twice by the Capping method, and from the resulting solution 1P {sulp is precipitated by an isoelectric method.
Осадок ипсул1П1а собирают и высунпп5ают .The precipitate of ipsul1P1a is collected and expelled.
Предмет изобретени Subject invention
Способ очистки технического ипсулина посредством осаждени балластпых нгелочью из подкисленного раствора, предварительно очищенного изoэлe тpпчecки переосаждением аморфного инсулина , и применени последуюи1,ен его перекристаллизации, о т л ич а ю пГи и с тем, что, с целью определени окончани реакцип осаждени балластных веществ, окраску раствора, наход и1,егос над осадком , сравнивают с окраской 0,001 нормального раствора пода в растворе йодистого кали .The method of purification of technical ipsulin by precipitating ballast nanoglobes from an acidified solution, preliminarily purified by means of reprocessing amorphous insulin, and applying subsequent recrystallization of the nucleus, in a melting point, and in order to determine the end of the deposition reaction the color of the solution, which is found above the precipitate, is compared with the color of a 0.001 normal solution of the solution in a solution of potassium iodide.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU446446A SU95262A1 (en) | 1952-04-28 | 1952-04-28 | The method of cleaning technical insulin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU446446A SU95262A1 (en) | 1952-04-28 | 1952-04-28 | The method of cleaning technical insulin |
Publications (1)
Publication Number | Publication Date |
---|---|
SU95262A1 true SU95262A1 (en) | 1952-11-30 |
Family
ID=48370369
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU446446A SU95262A1 (en) | 1952-04-28 | 1952-04-28 | The method of cleaning technical insulin |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU95262A1 (en) |
-
1952
- 1952-04-28 SU SU446446A patent/SU95262A1/en active
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