SU826584A1 - Vaccine against newcastle fowl disease, process for preparing the same and method for prophylaxis of newcastle fowl diease - Google Patents

Abstract

The vaccine against Newcastle fowl disease incorporates a virus-containing extra-embryonic liquid of a lentogenic strain Paramyxovirus a-1 "Bor-74 VGNKI" deposited at the All-Union State Research and Control Institute for Veterinary Preparations of the USSR Ministry of Agriculture and has a biological acitivity of from 108.0 to 109.75 EID50/ml. The method for preparing this vaccine comprises infectioning chicken embryos with the strain Paramyxovirus a-1 "Bor-74 VGNKI", preparation of a matrass breeding culture of the virus at the temperature of 37 DEG -37.5 DEG C. for 72-80 hours followed by isolation of the virus-containing extra-embryonic liquid and manufacture of the vaccine. The vaccine is employed for prophylaxis of Newcastle fowl disease by way of administration thereof once or twice onto mucous membrane of the fowl eye or nose in a dose of from 0.05 to 1.0 ml at the dilution of 1:100 or by way of enteral administration with potable water to chicks of an age of up to 25 days in a single dose of from 7.5 to 10 ml at the dilution of 1:1,000, followed by revaccination of the elder fowl, or by administration as an aerosol to the fowl of an age under 25 days in a dose of 300-400 EID50, followed by revaccination of the elder fowl in a dose of from 800 to 1,000 EID50.

Description

A method of making a vaccine against a guardian of a disease of birds from this strain includes infecting chicken embryos with a infectious dose of a viral suspension from a strain of the La Maltese poultry disease, replicating the virus for hours, obtaining an extra-embryonic virus, and csvieimJBamie, it will have an extra embryonic virus, and will have a csvieimJBamie virus, and csvieimJBamie. Whose dried (skimmed milk) packaging, amorajivashe and pyophilisate G31 A method for the prevention of Newcastle poultry disease includes the use of the vaccine against the Nykasas disease of birds from La Sota strain anazalkogo, enteral administration in aerosolized shga orgagazm ptiny sledutoshey with its revaccination f4 J.  The disadvantage of this vaccine is its iraroToBneviifSi method and the method of prophylaxis.  It is also the fact that the vaccine, which is made from the LaSota strain of the strain of the pathogen, is also insufficiently immunogenic, which also takes more time to produce, and its use causes a breakthrough of immunity in the inoculated animal and its succession in the event of an accident. disease.  The aim of the invention is to increase the immunogenicity and safety of the vaccine, shorten the time required for it, as well as increase the effectiveness of prophylaxis.  This pell is achieved by the fact that the vaccine contains a virus virus as a vaccine strain.  Pa ha t-h about virus a-1 Bor-74 with the following composition of the vaccine, weight. %: Extraembryotal fluid VS3 strain Pdrai-nyxovirus a-1 Bor-74 with a biological activity of 10 I L EID50 / ML50-55, Protective environment 45-50 The vaccine contains antibiotics, mainly penipyllia and streptomycin in blood 1OO-200 U / ml vaknn For infection of chicken embryos, the strain pdranrsxoViros a-1 Bor-74 VGNKI is used at a dose of 10 ° -10 EID50 / 0.1 ml, and the paaviHOKemie virus is administered for 72-80 h.  For the prevention of Newcastle disease of birds, io use a vaccine containing extra-embryoiacal fluid from the strain.  RA 8 4 bio-activity 1 O. o-YuEIDO / ML in the amount of 50-55 weight. % protective environment in the amount of 45-5OB and antibiotics, mainly sang LIN and streptokheshdn in the amount of SO 200 EL / ml, a vaccine that is administered i the body of the bird intranasally (or on the conjunctiva of the eye) at a dose of 0.05-0.1 k and dilution 1; 10О-1: 10OO one sludge twice, or ekteralno with drinking water in a dilution of 1: 100O-1: 5OOO chickens there 15-25 days of age at a dose of 7.5-1O, 0 ml once with after; Older bird revaccination at an older age at a dose of 10, 0–15, O ml, or a 15–25-day-old bird to a sily at a dose of 300–400 EIDzo / followed by revaccination. birds in older age at a dose of 80O-120O EIDBOV as a vaccine slurry xpx strain of Horn m: 3xov-ihiba-1 Borogngniki, which is characterized by a trace of LEADING signs and properties.  Morphological signs.  When viewed in an electron microscope, there are clusters and individual particles, mainly filamentous and racket-like forms of virions, as well as spherical and polymorphic particles of this virus.   The envelope of the virions is covered with a set of small catholic protrusions 8C 100 A long, with a diameter of about 30 A.  They Bi step above the surface of the cap and t stand a hundred from each other at 70-8 ° A.  In the form of virions, the styloid forms are located on the hull of equal shape or regular rows.   Strain. Bor-74 EGNKI basically has intact phoumas of virions.  The 3mv property of the dashdl strain is different for the oi of other vaccine strains (Bx, LaSota) of the Newcastle disease virus.  In some cases, the strain Bo1 -7 VGNKI detects a thin filamentous helical structure with a regup rns with a frequency of 5O A.  along its base  Its diameter is about 170 A, the diameter of the canal is 40-50 A.  This structure is the ribovleoprotein of the virus.  CJ is similar in structure to other shgayma virus ngokaslskoy disease.  For the Bor-74 strain VGNKI harvk terEO is less compared to the strain La-Sota n. the number of spherical forms of virions (size 9O-15Onm), compared with more filamentous forms of virions with a diameter of 1OO-15O nm, a length of 1.5 nm, less often racket-like forms. Flax culture is indicative.  Bvipyc is well bought in 1–10 day old chicken embryos without causing their death during a 96 hour incubation time at ST ° C, Tert rsgstabile Susceptibility, to the torso of the Bor-74 strain VGNKI Bospormatichy chicken embryos and all healthy grits of all 74 gates.  Virulent and antigenic properties.  Strain BO5 -74 VGKKI avarupenten for ptvd all age groups.  Fired to a group of tape-strains, Adaltrova to the respiratory organs of birds.  It has the ability to preserve nature. It is capable of transmitting to the environment and ways.  In susceptible ptsh, it causes the formation of blind antibodies, ingkbir YuEth 1Hemagtlu-gacketsho and neutralizing airus of the reference strains of khkzhaslskoy disease virus. The virus reacts with antibodies obtained to gokolnnnmu in the strains of the Newcastle disease crucible of birds.  With the introduction of a susceptible, it causes the formation of a strained kmmukitta against the epizootic virus of the nyopaopaemia of the sore biodiversity. 5 „1З3зД50 / ml, Immunogenic activity (5O% -a); Phi intranasal method of administration 2.93 EID 50/01 ml when discharging 4.97 AID 50 / 10.0 ml with drinking water; .   with the aerosol method, injected for chickens up to 25 days of age 300-400 EIDzo D adult 8001200 EKZO.  The recovered levels, once vaccinated from the Bor-74 strain VGNKI using the local and enteral methods of administration, 7 days after vaccination and 4 38 days were immune to 100% of cases (observation period). Serological properties.  The virus of Boron-74 strain VGNKI has the ability to agglutinate erythrocytes of PETE and animals.  Gives pronounced spontaneous agglutination with the blood of chicken embryos.  Hemagglutinin nitrate 1: 512.  The reaction of the hemagglutine is fine-grained and manifests slowly.  Vaccine against Newcastle birds as a vaccinated strain.  contains the strain of the virus Pc g "t hovirus a-1 Bor-74 VGNKI with the following Vtfusssoderzhan extrastrambrconal LIQUID from the strain Ragagp-a xovirus-1 Bor-74 VGNKI with the biological active & EIDZO / ML50-55 Protective environment (dez.  milk fat) 45-5O Antl5biotics, mainly pennpil LIN and streptomycin 10O-2OO U / ml.  Example 1  Virus-containing material Zapstnaz medium Antibiotics for 1OO-2OO U / ml Example 2.  The vcrc-containing material Zagzitv iOO-200 U / ML Anti-Bottling Medium, Vakoivu is prepared as follows: E-brioks are incubated prior to their infection and incubated according to the zootechnical requirements for ELECTROLY chickens, KOTOXYX, infected at 10 days of age, in the Alantic cavity, the susceptible chickens, KOTOXYX, are infected at 10 days of age, and the susceptible chickens are infected in the Alantic Cavity at 10 days of age, they are infected and they have been infected in the 10 year old chickens, they are infected in the Alpine chickens. Param-axovtrosa-1 Bor-74 VGNKI at a dose of 10 "° -10 OEDD50 / ODML.  and the multiplication of this virus is carried out for 72-8 oh, at a temperature of 37 + ± and relative humidity 6 o 7 o%.  Infected embryos are scanned with an ovoscope once a day for 3 days.  All embryos that died before 72 hours are rejected. .  For live use, only live embryos of 72-80-hour kncubanag are used, which are pre-cooled at -J-4C for at least 8 h.  Opening the embryos is kept for 2--3 hours at room temperature until the condensate (moisture) is evaporated on the shells. The embryos are opened up under strict aseptic conditions, they remove the membrane from the shell and suck off allantoisio-microalterobactile {extraembrybride) with sterile eye tweezers. which is poured into a sterile non-dermal vial with a capacity of 10 ml.  In each flv-con an acstrambryonic fluid is taken from 8-10 embryos.  Each extra vial fluid of the vial is seeded with a sample of MPL, MPB, MPB under vaseline oil and Saburo medium.  The seeded media was kept in a thermostat at and 20–22 ° C during an extraembryosol liquid test for sterility (in the bacterial outlet) bottles with the virulence containing material were immediately removed and dried at i-4C.  One of them carried out a preliminary inspection of three bottles from partch (2 ml each) in the PTA (the tiger should not be shocked 1: 256).  To make a splash of blood, they use only bacteriologically apaitic and amniotic fluid, jvOTOpyra is poured into one vessel under osheshpik conditions, mixed in equal proportions with sterile skim milk, added with antibiotics, and asparanters are served. 100-200 U / ml vak- / Sh1NY and joke round for 10-1S min. After the jester has been cleared, vaccinated vaccinates (vc), the suspension is poured into sterile vials of 4 ml, into ten tiimishlifovye fgcons at 6) W1.  . Ampoules And flakoshg with the drug, it is enlarged with a double-layer gauze napkin or a sterile hygroscop with cotton wool, which is frozen in shzatlemetratnyh apparatus at a temperature of 40-50 0 for 6-10 h (in the case of "Eee relatively large waste glass ampoules at a temperature of 6-10 h virus-containing suspension of freezing of npo BODYAG in two stages - first at 20 ° C for 4-6 hours and then at 35–40 ° C for at least hours, only 14–18 hours for necessarily switched on fans);  The frozen vaccine is loaded into the drying vacuum chambers quickly (ke for more than 2–3 min), without allowance for thawing of the biological product.  Freeze-drying is carried out at a gradual Kovyshentgl temperature up to 23 ° C for hours. (about the amphibian vaccine series 20 AOL).  At the end of the pyophilzapine, under sedated conditions, amdui is sealed and the bottles are sealed with caps with a continuous control of all the vodka on the surface, harmlessness, immunogenicity, residual moisture and biological activity of emryhovahs.  The safety of npoBepsaoT is 17 "DEBFSxK susceptible ways, see  intramuscular injection of vashni in the volume of 0, 2. "I from a dilution of 1; 1O.  Nabpk after chickens held during 3. 4 days.  Immunogenicity of vaccines is determined by 1 17-day-old chickens with an intuasal (1 ml of 1: 1 dilution OO and 1r-H) D) O1 tr a. ,,. - f-- of the derivations 1: 1OOO and 1: 5OOOO) methods of learning.  The presence and intensity of immunity in all chickens from the experimental and control groups was tested 14 days after vaccination by infection of the virulent strain. T of the Newcastle BS virus in a dose of 1000 LL50 / O2 ml. Monitoring of an infected bird was carried out for ten days.  All experienced, vaccinated with the indicated methods, after controlling infection with the virulent-regularm T virus of the Newcastle disease virus, were immune when 1% of the control table (see  tab.  one).  In six experiments, the immunogeic activity of three series of dry virus vaccine from Igta LMA Bor-74 VGNKI on 17-day-old chickens using the hctranasal and enteral method was determined.  vaccination from dilutions of the virus from 1O to 10-8.  Using statistical processing methods, determined. immunizing activity (IMDzo) for each vaccine series in icctranasal and 9-unit immunization method.  .  Immunizing activity: Seri-1 with the initial biological activity of 10® EID50 / ml with n-transranal vaccination method was 3.6 log.  EID50 / 0 1 ml of P ectral vaccination method - 5.2 log.  ER1D50 / 1O, Oml; Series 2 with the initial biological activity of U EIDzo / ml was 2, 4-lor.  5ID5O / O, 1 ml and 5.0 log.  AID50 / 10.0 ml, respectively; Series 3 with the initial biological activity of IDZO / ml RI and the second method of administration was 2.8 log.  EIDdud ,, and with an extra 4.7 log.  ZID5O / 10, OmdPo According to the results of six experiments, the 5O% w immunization activity (IMDYO) with the nasal nasal administration method was 2.93 log per dilute, ERSCH50 / O, 1 ml, and in the enheral-4.97 log. EID5AO, Oml (zl.  tab.  2).  .  Danvu.  A vajivu containing extraembryoglunK LIQUID of KZ of the strain Ragchmcexovirus a-1 VGNKI with the bioelectric activity of ZO / MP in the amount of 5О-55 wt. % protective environment in the amount of 45-50 weight. % and antkbiotnkv, most often Petshillvn and streptomycin in the amount of 1OO20O U / ml, vaccine quppot. - the purpose of the Newcastle disease is intranasal (itg on the conjunctiva of the eye) in Doway O, O5-0.1 ml and dilution 1: 100 1: 100 About one or two puvratio Shga entero only with 1TopaBoy in 1: 1OOO1: 5000 chickens 15- 25 days of age at a dose of 7.5–10.0 ml once, followed by revaccination of PTCA at an older age at a dose of 1O, 0 ml or a carrot bird 15–2 b, at a dose of 300–4 OO EIDzo, followed by revaccination of a bird at an older age at a dose of ÖOO-120O EID50  The results of each vaccination are monitored after 14–15 days by serologic testing of blood in the HRA, followed by at least 25 blood samples from each batch of PT0C.  A bird is revaccinated if 20 to more percent of blood samples have an antibody titer lower than 1: 8o. At two poultry farms in the Moscow Region, 4 million poultry were tested on each of the recommended methods to administer a dry vaccine virus from PCfrotmyxovtrus a-i Boiv. 74 VGNKI.  Example.  Four batches of chickens (but 15-16 thousand  heads c) vajevny intravascular vax method & Noh of the La Sota strain from the scout 1; 25 in a volume of two drops at 12–13 and 30–32 days of age.  Thus, 63.7 thousand were vaccinated.  heads  Twice iad ia irauJT batches (13-16-16 thousand each) were vaccinated with the intranusal vagrin method — from the VGNKI strain at a dilution of 1: 10O in the amount of two drops at the age of 12-13.  In this way, 169.4 thousand were vaccinated.  heads  Two batches of 13-day chick-ups (13 thousand each)  in each), the vaccine of the boron strain of the Bor-74BGNY strain was diluted by dilution 1; 1OOO in the United States, O ml.  Viruacavid was drunk for one day.  This method was vaccinated 26.4 gys. heads  At the same time, parallel postavihsv experiments to determine the possibility of Spo.  secret vaccination of the joint content of vaccinated and non-vaccinated chickens.  From this point of view to the production batch of TSPNF, the vaccine {enterally, 1.5 hours after the immunization, the anomalies of the radiation source were planted. chicks of the same lot.  Two batches of 13-day-old chickens (13 thousand each, each of them were vaccinated with intras using the La Satiy CG strain of 1:25 in a droplet volume of 1 drop of 4 drops.  Then, at 32 days of age, one batch of chickens was revaccinated by the same vaccine at a dose of 150O EID50 per head, and the second one with a vaccine from the Bor-74 strain of the All-Russian State Medical Committee of Russia. TIM using the same method also in a dose of 1500 EID20.  The exposure of the bird to aerosol exposure was the same in the composition of 15 minutes.  The exposition was read out 3 minutes after the work of the aerosol generators.  Behind all the experimental batches, the games were conducted on a regular basis and every day was carried out at a pathoanoyatom opening of the exhibition.  None of the experimental pvrtvk chickens showed a visible external reaction to the introduced antigen.  Chicks were mobile, willingly ate food, and normally developed Post-vaccination pedsmno (dull, wheezing 9 difficulty breathing) on amp day on the sixth day, only isa batches of chickens revaccinated by the aerosopic method of vaccine of the LaSoga strain.  The response of the vaccine to aerosol administration of the vaccine from the Bor-74 strain VGNKI was not observed.  The range of chickens from 1 to B-dkevne age was in the range of 96,698, 3% at 95% plan.  The average daily weight gain of pullets from 1 to 60 days of age was in pr & cases 9.5-1O, 4 g with a melt of 9.5 g  Summary data on the presence of passive and active immunity in experimental birds are presented in Table 3.  As can be seen from the data table.  Level 3 of passive immunity in the experimental groups of ting was mostly the same (3.4–3.6 norj).  The titer of avtigel in the first group of Iyp of the 15th day of the 13-day-old Intro vasal method of the La Sota strain vaccinated 14 days after vaccination was 4.0 log.  2 after the second vakvvshgegei (after 14 days), he rose to 5i5 log.  n to 21 dsho was 5.1 log. The secondary vaccine of the 32-day long-term 0TOU group of the LaSota strain was caused by a high level of antibody after the first vaccine.  The titer of the autorun y-2, 3 4 groups rolls, greetings at the age of 13 years old into the mesa of nococuncture of the eye in drinking water with the vaccine taken from the Bor-74 strain VGNKI, after 7.17 in 14 days glory ee-tr "r. .  -c and by 21 days it reached 6.3-6.9 lpg. . , and for 42-45 days, it was within 5.5-6. At the same time, the tiger of antibodies was more pronounced in chickens vaccinated with the vynaBitam vaccine with drinking water (6.9 LOG. ;)  Tvr antibodies in spontaneously vaccinated chickens (group 5) after 14 days was 5.7 log. by 21 days it reached 6.3 LOG. 2 and 42 days later, it was 5.2 log-2 In the sixth and seventh groups of the sixth and seventh groups, 1: 1 In the 13th day of the whole year by the intranasal method, the vaccine from the Jla-CoTa -f strain after 14 days had an antibody titer in 5% , 45, S to the sixth group of students who were secondarily inoculated at 32 days of age with the vaccine of the Lag-Sota strain by the aerosol method, after 14 days it was 4.1 log.   and after 21 lek AOCTWr 7, O log. - In chickens Enopopagnoy (group 7), vaccinated with a vaccine mz tgamma Bor-74 1 using the aerosgam method, an Tvtel titer was soyly light 6.4 and 7.1 log Reactive control of onuTiiDbix infection and some of the roller groups of pretzel in tab.  four.  All groups of birds vaccinated with vaccine from Bor-74 VGNKI and Laksota strains were immune in 100% of all control (non-immune) chickens (with the exception of group 6, where after control of Hoi-t) the infection fell one strand .  The newly released workshops of industrial herds of chickens after appropriate treatment to de. The knowledge of the repair pullet was calculated taking into account the strain j of the virus previously used for vaccination of the method of its introduction.  .  All experiments} 1st pear chickens previously vaccinated in the 13th vaccine from the Bor-74 VGNKI strain are revaccinated at the age of 60-b5 with the same vaccine.  Antibody titer after 14 days of nooie second vaccine composition 7.3 log. p, after 4 days S. Snor (period nabgaudesh1).  "I 41 ,.  The monitoring of chickens vaccinated with a dry virus-vaccine from the VGNKI strain carried out within 1 days showed that it was harmless and high jOvnvfyKoreKHDCTb.  .  When vaccinating a child by the indicated methods, the immunity comes on the sixth to seventh {oh day in 95-100% of cases.  The duration of immunity in once vaccinated birds is up to 438 days (observation period).  In contrast to the dry virus-vacnine off strain of La Sota, the dry virus-vaccine from the Bor-74 VGNKY strain, has more pronounced immunogenic properties (on l, 58i) and the property of contact immunization.  When immunized with drinking water, the plate is vaccinated once, the reaction to the aerosopically injected antigen occurs 2 days earlier (this PROPERTY of the virus is very important for vaccine birds in a dysfunctional household).  The use of the vaccine from the Bor74 VGNKI strain allows to reduce the multiplicity of poultry vaccines and the consumption of the vaccine.  The techno-economic effect of changing the dry virus vaccine against Newcastle disease from the VGNKI strain will be due to: creating tense immunity in birds in disadvantaged and prosperous areas of the country (thereby significantly reducing the incidence of pti); reducing the frequency of vaccination pnpy; a fourfold reduction in the consumption of chick embryos (250 ml of nasal doses are contained in 1 ml of round strain of the La Sota strain, and 100O nasal doses of the Bor-74 VGNKI strain); Creation of a term for the technology of prokbodstva dry virus vaccine vzggamma Bor-74 VGNKI; reducing production scams. Tey (Ex) for the manufacture of vaccine against Ayuskal disease (one enterprise of the love industry will be able to produce 4 times more vaccine from the Bor-74 VGNKI strain at the same costs as the LaSota staff) vaccine.  ,

The results of the test in the immunogenicity of experienced serki dry vaccine from the Bor-74 strain VGNKI

Table 1

The results of the determination of the 5O% immunosuruchy d | PS three series of vaccines of the boron strain-74 VGNKI

Control (n & vaccinated)

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“And H J.98 Formula acquired by 1.Vakshtnn against Yulaklova biopsy of the wild animals, 133 of the strains of St. John's virus, and the environment of the milk from the warmer milk, so that, with the aim of increasing the amount of milk, it is necessary to increase the amount of milk from the“ warm milk ”, so that, in order to increase the intensity As a vaccinate strain of Param rJETGMM virus: (oVfrus a-1 Bor 7-4 VGNKI iij3H with the following composition of the vaccine, kes,% Extraembryonic fluid 1 "w of Parc" mvxov ros al Bor-74 VGNKI with biometric bandwidth, and there is quite a way that, if you are using a child, you will not be able to do this, you will be using a firewall, you will be able to take advantage of the same time, and you will be using a child and you will not be able to do this, you will be able to use a place to do this, you will be able to use a default, and you will be using a child-friendly way to do this). / ml SO-55 Protecting medium 45-5O 2. Wakikna against weather for the disease of birds of 1TO p. J., tp and 10a and the fact that., it contains antibiotics, penicillin prematurity and creptometry in a quantity of 100 "-2OOU / ml of vaccine .3. The method of preparation and Nakhodka Nakhodka, the first place in the world; u and with hem, that, in order to shorten the time of her izg tovleni and higher audio} 1MMunogeinyh properties .vakiigsht, DLG zarahseii embrioiov chickens Using T suspenztpo virus strain that Pararn jco f 03 a-l-GZor 74 VGNKI. The IDo / OD mlncnccccccassion of the cranberry is carried out within 72-80 h. 4. The method of preventing "Ayuklänsky ptshg disease, including the use of the vaccine against Newcastle ltid's disease by its intranasal, enteral or aerosol introduction into the body of the bird with its subsequent revaccination, characterized in that, in order to increase the effectiveness of prophylaxis, the vaccine used is given to obtain an extrinsic gasmicrobial liquid taken from the Pa ra HLUHO virus a-1 Bor-74 VGNKI with a biological activity of 10®. EID50 / ML in the amount of B (-55 wt.%, Protective medium in the amount of wt.% And antibiotics, mainly penidillium and streptomycin in the amount of 10О-20О ЕЛ / ml of the vaccine, which is introduced into the body of the bird and nasal-nasal (or va conjunctiva eyes) at a dose of 0.05-0.0 ml and dilution of 1: 1OO-1: 1OOO one or two times, or enterally with drinking water in a dilution of 1: 1OOO-1: 5OOO chicken 15-25 days old at a dose of 7 , 5-10.0 ml once, followed by revaccination of pt1y at an older age in a dose of 1O, O- 15, O ml, or an aerosol bird of 15-25 days old in a dose of ZOO "-40O 2ID5O elimination of older individuals at a dose of 80–1,200 EID50Sources of information taken into account during the examination 1. Instructions for the manufacture and control of a dry virus vaccine against pseudo-plague Ltcc from a strain approved by the Government Department of the USSR 10.09.73 2. Application on dry .jBKpyc vaccine against Nyshsl's disease .Evicants from strain, approved by GUY jyiCX USSR O9.O6.78. 3. Tools for the production and control of dry virus wakiena against PSA and birds from La Sota strain, approved by GUV MSH USSR 1O.O9 .73. 4.Provision for the control of a dry virus vaccine against Newcastle disease from the La Sota strain, approved by the USSR State Medical Union on 09.06.78.

Claims (4)

Claim 1. Vaccine against Newcastle disease "a type containing extraembryonic liquid from a strain of Newcastle Virus 5 clear disease and a protective environment from unbroken milk, which is characterized by the fact that, with the aim of increasing the extent of harmlessness of the vaccine, it is a vaccine strain contains ¹⁰ strain of Rag virus myxovirus a-1 Bor74 VGNKI with the following vaccine composition, wt.% Extraembryonic fluid from strain Paramyxovirus a-1 Bor-74 VGNKI 'with biological activity 10 ^b ' ° -10 ⁹ · ⁷⁵ EIDbo / mp50-55 Protective environment45-50 2 "Vaknina against Newcastle disease - ²⁰ no birds on π. 1, characterized in that. it contains antibiotics, mainly penicillin and streptomycin in the amount of 100-200 U / ml of the vaccine. '. ²⁵ 3, A method of manufacturing a vaccine against Newcastle disease of birds, comprising infecting chicken embryos with an infectious dose of virus suspension from a strain of Newcastle disease of birds, propagating 30 viruses, obtaining virus-containing extra-embryonic fat, mixing it with a drying medium, packaging, emotion and shufilization, characterized in order to reduce the time of its manufacture and increase the immunogenic properties of the “vaccine, for. '; infection of chicken embryos using a suspension of the virus from the strain Paranwxo- + goz a-1 Bor-74 VGNKI 10 ⁴ '° -10 ^ ° ¢ 0 ZID5o / od ml. ^a. Reproduction of this virus is carried out for 72-80 hours 4. A method for the prevention of Newcastle disease of PTI, including the use of a vaccine against Newcastle disease of 45 birds by intranasal, enteral or aerosol administration into the body of a bird with its subsequent revaccination, characterized in that, in order to increase the effectiveness of prevention, they use a vaccine ekstrazmbrionalnuyu liquid of Paramyxovirus strain a-Bor-1 74 VGNKI with biological activity 10® · ° -1O ^{9 '15} EID50 / ml ⁱⁿ an amount of 50-55 wt.%, the protective medium in an amount of 45-50 wt.%, and antibiotics, preferably penis lin and streptomycin in an amount of 100-200 IU / ml of vaccine. which is introduced into the body of a bird intranasally (or on the conjunctiva of the eye) at a dose of 0.05-0.1 ml and a dilution of 1: 1001: 1000 once or twice, or enteral with drinking water at a dilution of '1: 1000-1: 5000 chickens 15 -2 5 days of age at a dose of 7.5-10.0 ml once with subsequent revaccination of poultry at an older age at a dose of 10, ΟΙ 5.0 ml, 'or aerosol on a bird of 15-25 days of age at a dose of 300-400 EID50 ^followed by a booster birds in older age at a dose of 800- 1200 EID about ₅ ·