SU819169A1 - Chamber for simultaneous microscopic and electrophysiological investigations of living tissue and cell preparates - Google Patents

Description

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When using a known camera for working under high magnifications of a microscope, a number of its design flaws and inconveniences in operation are also found. The cover glass, which serves as the bottom of the chamber, is thin and fragile. It is often pressed from below when in contact with the metal parts of the microscope stage or from above with a slight refocusing of the large lens. Drug change during operation requires removal of the entire chamber from under the microscope, which disrupts laborious setup of the duct system, external electrical contacts, etc. In addition, the presence of ferromagnetic magnetized materials in the chamber design affects the electrical properties of the drug and distorts the measured parameters .

The purpose of the invention is to expand the functional capabilities of the camera due to the differential diagnosis of the morphoelectrophysiological state of all the components of the nerve conduction: cat fibers, presynaptic terminals and postsynapses.

This goal is achieved by the fact that the chamber is equipped with additional, at least two macroelectrodes, the latter being double-barreled and placed around the circumference of the hole on the spherical supports made of plastic, viscous and heat-resistant substance, and the device for fastening the preparation is placed At the bottom of the chamber there is a flat frame with clamps fixed perpendicular to its horizontal surface and has guides for insertion of an external electrode, the inlet of the channel is suction a is located above the outlet of the feed channel, with both channels fitted with wicks. In addition, the frame is made of organic glass, and the tabs are made of tungsten.

FIG. 1 shows a general view of the camera; in fig. 2 is a general view of the device for fastening the preparation; in fig. 3 - section A-A of FIG. 2

The chamber has a glass thin bottom 1 and legs-supports 2. The glass is attached to the Plexiglas body 4 with an opening of conical shape with the bottom 1, which forms the capacitance of the physiological saline chamber together with the bottom. The edges of the camera are gentle. The fixture 5 for fastening the drug is made in the form of a plexiglass ring, in it around the circumference there are fasteners 6, which are tungsten pins with pointed ends, some of which are bent in the form of hooks. In the slots of the housing 4, the feed channels 7 and the suction 8 of the saline solution, made of injection needles, are provided with wicks 9, which sink into the container chamber. The wicks can be made of filter paper or cotton thread. The outlet of the suction channel 8 is located above the outlet of the feed channel 7 to provide a certain level (depth) of solution in the tank. A circumferential indifferent electrode 10 made of platinum or chlorinated silver is fixed around the circumference of the chamber. The attachment device 5 is attached to the di 1 tank with a lump of viscous plastic mass.

Double-barrel coaxial suction macroelectrodes 11 are placed (supported at a point) with the possibility of moving on ball bearings 12 from viscous

plastic mass with a diameter of 4-8 mm. The mass retains its properties and shape at the heating temperature of the illuminator.

The conductors from all electrodes converge on a special Plexiglas plate (on

The drawings are not shown), which is mounted above the main plate of the housing 4 for the purpose of waterproofing the contacts from the chamber container. The plate serves as a plate, inside which the sockets are inserted for five

seven connectors.

The camera works as follows. The preparation is fixed on the fixture 5 for fastening the preparation under an auxiliary binocular microscope (such as

MBS), which can be separate or fixed near the main inverted microscope and focused on its table. At first, with medium magnifications, the drug is oriented in a frame. Then under

By the lens 4, it is fixed on the pins and hooks of the fixer 6. After that, the device 5 for fastening the preparation is turned over and placed in the chamber reservoir so that the preparation appears

below and adjoined to the bottom of the chamber.

Include flow system. By changing the height of the standing or the inlet of the suction needle, they regulate the level of the fluid in the chamber. The wetted wicks 9 touch the surface of the liquid. Move the tips of the movable suction double-barrel coaxial macroelectrodes 11 (controlled through a magnifying glass) to the desired nerve fibers. The electrodes are connected to the stimulator and analyzing electronic equipment through the block of the camera.

Then the camera together with the table of the microscope is moved to center the necessary part of the object (neuron) in the focus of the microscope. Next, an external glass microelectrode (not shown in the drawings), which was centered before the focus of the microscope and

retracted by micromanagement torus

Claims (2)

Claim 1. Camera for simultaneous micro-optical and electrophysiological studies 5 forms of live tissue and cell preparations: a current containing a housing made of biologically inactive plastic with an opening for placing the drug and a glass bottom, a macroelectrode, a device for fixing the drug in the chamber and channels for supplying and suctioning physiological saline, characterized in that, in order to expanding the functionality of the camera due to the differential diagnosis of the 5 morphoelectrophysiological state of all components of the nerve conduction: the meat pulp, presynaptic terminals and postsynapse, it provides wife with additional, at least two, O macroelectrodes, the latter being double-barreled and placed around the circumference of the hole on spherical supports made of plastic, viscous and heat-resistant substance, and the device for attaching the drug is a flat frame placed on the bottom of the chamber with reinforced perpendicular to its horizontal surface with clamps and has guides for introducing an external electrode, the inlet of the suction channel is located above the outlet of the channel cottages, wherein both channels are provided with wicks. 2. Camera by π. 1, characterized in that the frame is made of organic glass, and the latches are made of tungsten.