SU552538A1 - Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations - Google Patents

Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations

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Publication number
SU552538A1
SU552538A1 SU1956266A SU1956266A SU552538A1 SU 552538 A1 SU552538 A1 SU 552538A1 SU 1956266 A SU1956266 A SU 1956266A SU 1956266 A SU1956266 A SU 1956266A SU 552538 A1 SU552538 A1 SU 552538A1
Authority
SU
USSR - Soviet Union
Prior art keywords
leukocyte
cationic protein
detecting free
microscopic preparations
microscopic
Prior art date
Application number
SU1956266A
Other languages
Russian (ru)
Inventor
Елена Александровна Венглинская
Борис Иванович Рукавцов
Моисей Григорьевич Шубич
Original Assignee
Кубанский Государственный Медицинский Институт Им. Красной Армии
Кубанский государственный университет
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Кубанский Государственный Медицинский Институт Им. Красной Армии, Кубанский государственный университет filed Critical Кубанский Государственный Медицинский Институт Им. Красной Армии
Priority to SU1956266A priority Critical patent/SU552538A1/en
Application granted granted Critical
Publication of SU552538A1 publication Critical patent/SU552538A1/en

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Description

33

лейкоцита. Установив изображение лейкоцита в области фотометрической диафрагмы, снимают показани  гальванометра (в условных ед.). Подобным образом фотометрируют 100 клеток, после чего вычисл ют среднее арифметическое интенсивности флуоресценции (в условных ед.) на одну клетку. Так как при этом получают информацию о количестве катионного белка в условных единицах, полученные данные сравнивают с данными исследовани  крови контрольной пробы.leukocyte count. Having established the image of a leukocyte in the area of a photometric diaphragm, take readings of a galvanometer (in conditional units). 100 cells are photometrically similarly, after which the arithmetic average of the fluorescence intensity (in units) per cell is calculated. Since this provides information on the amount of cationic protein in arbitrary units, the data obtained are compared with the blood test data from the control sample.

Предлагаемый способ позвол ет быстро определить содержание цитоплазматического катионного белка в лейкоцитах, что  вл етс  важным показателем функционального состо ни  этих клеток.The proposed method allows rapid determination of the cytoplasmic cationic protein content in leukocytes, which is an important indicator of the functional state of these cells.

Claims (1)

Формула изобретен и siFormula invented and si Способ вы влени  свободного цитоилазматического катионного белка лейкоцитов в микроскопических препаратах путем вз ти  крови , приготовлени  мазка, фиксации, промывани , окрашивани , повторного промывани  и микроскопировани , отличающийс  тем, что, с целью повышени  точности и уменьшени  времени анализа, окрашивание провод т примулином при рН 8,0-8,2, фиксируют раствором сульфосалициловой кислоты, промывают в боратном буфере, микроскопируют с помошью люминесцентного микроскопа, а количественное определение провод т с помошью цитофлуориметра.A method for detecting free cytoylazmatic cationic protein of leukocytes in microscopic preparations by drawing blood, making a smear, fixing, washing, staining, re-washing and microscopic examination, characterized in that, in order to improve the accuracy and reduce the analysis time, staining is carried out with primulin at pH 8.0-8.2, fixed with a solution of sulfosalicylic acid, washed in a borate buffer, microscoped with a fluorescent microscope, and quantitated using cytopha uorimetra.
SU1956266A 1973-07-31 1973-07-31 Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations SU552538A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU1956266A SU552538A1 (en) 1973-07-31 1973-07-31 Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU1956266A SU552538A1 (en) 1973-07-31 1973-07-31 Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations

Publications (1)

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SU552538A1 true SU552538A1 (en) 1977-03-30

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SU1956266A SU552538A1 (en) 1973-07-31 1973-07-31 Method for detecting free cytoplasmic leukocyte cationic protein in microscopic preparations

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0562877A2 (en) * 1992-03-27 1993-09-29 ORTHO DIAGNOSTIC SYSTEMS INC. (a New Jersey corp.) Cell fixative and method of staining cells without destroying the cell surface
WO1998003875A1 (en) * 1996-07-24 1998-01-29 Helena Laboratories Corporation Reagents and methods for fluorescent analysis of serum proteins

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0562877A2 (en) * 1992-03-27 1993-09-29 ORTHO DIAGNOSTIC SYSTEMS INC. (a New Jersey corp.) Cell fixative and method of staining cells without destroying the cell surface
US5597688A (en) * 1992-03-27 1997-01-28 Ortho Diagnostic Systems Inc. Cell fixative and method of analyzing virally infected cells
WO1998003875A1 (en) * 1996-07-24 1998-01-29 Helena Laboratories Corporation Reagents and methods for fluorescent analysis of serum proteins

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