SU1511659A1 - Apparatus for electrophoresis in gel vertical plates - Google Patents

Abstract

The invention relates to the field of laboratory technology used for the purification and fractionation of proteins, nucleic acids, and the like. The purpose of the invention is to simplify the design of the device, expanding the field of application by ensuring stable operation in preparative electrophoresis with low concentration gels. The design of the device is simplified by using a block of gel cassettes formed by alternating open-top cuvettes = heat exchangers 7 with spacers 8 placed between them, determining the thickness of the gel plates 6, which allows the gel buffer of the upper electrode compartment 2 to be cooled. Presence of perforated bottom 5 of the rectangular upper electrode compartment 3 of the device allows working with thick (5-15 mm) and low (50-60 mm) plates of low concentration gel (5-7%) and excludes a slide gels of cassettes. 5 il.

Description

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The invention relates to a laboratory technique used for the purification and fractionation of proteins, enzymes, nucleic acids and other high molecular weight compounds, and the scale to be used in biology, chemistry and medicine,

The purpose of the invention is to simplify the design of the device and expand the application area by ensuring stable operation in preparative electrophoresis with low concentration gels.

compartments 1 assembled two electrodes 9 parallel to each other, the plug S of which is led to the side wall of the lower electrode compartment 1, the other two electrodes 11 are mounted in the cap 12 of the upper electrode compartment 2, and their plug 13 is pulled out on the attachment 12. Top electrode compartment 2 mounted on special protrusions 14. For filling the cassettes 6 (FIG. 3) with a gel-forming solution and for sealing the rectangular recess 3

FIG. Figure 15 shows a device 15 with a cone-shaped plate 15 with a recess electrophoresis in vertical gel plates, a longitudinal section; in FIG. 2, the same cross section in section A-A in FIG. 1; on fig. 3 - the top of the upper electrode section is with the plate for pouring the gel-forming solution into the cross section (in section AA); FIG. 4 shows the upper electrode compartment without a side wall.

The proposed device consists of a lower 1 and upper 2 electrode compartments. The upper electrode compartment 2 is provided with a rectangular recess 3. Along the perimeter of the upper end of the recess 3, an elastic cuff 4 is installed. The bottom of the rectangular recess 3. is perforated in the form

16 and a hole with a nipple 17. A rubber gasket 18 is fixed along the perimeter of the plate 15. The plate 15 is attached to the bottom of the upper

20 of the electrode compartment 2 using spring clips 19.

The device works as follows.

Block gel cassettes, educative

25 alternating open at the top of the cuvette-heat exchangers 7 with spacers 8 placed between n1-1s, determining the thickness of the gel plate 6, are placed in a rectangular recess 3

30 of the upper electrode compartment 2. A plate 15 is pressed against it and secured with the help of chemis 19. The hole with fitting 17 is connected to a container (not specified) from the gel electrode 30 of the top electrode compartment 2. A plate 15 is pressed against it. and fix it with the help of chemicals 19.. The hole with fitting 17 is connected to a container (not indicated) from gel pens 5, the length of which is equal to the melody distance by the extreme plates 6 with a helium solution and bottom fill (Fig. 2). Cassettes 6. If necessary

A gel block is inserted into the rectangular recess 3. the cassettes for the gel plates 6, formed by alternating open-top cuvette-heat Q. the gel-forming solution is poured with pipetto-exchangers 7c interposed into one of the cassettes 6, from where it is spaced along them 8. The tight cuff is reached by the elastic cuff - heat exchangers 7 to the gaskets 8. The thickness of the gaskets 8 is defined by the thickness of the plates of the gel 6 (for analytical electrophoresis 0.5-2 mmJ for preparative electrophoresis 5-15 mm). Two pads 8 with

filling the cassettes with 6 homogeneous gel is used in a simpler way: the opening with fitting 17 is closed and

the grooves 5 of the perforated bottom of the rectangular recess 3 flow into other cassettes.

In this case, the gel-shaped solution also fills the gaps between the walls of the rectangular recess 3 and the heat exchanger cuvettes 7, and this seals the upper electrode compartment

the adjacent walls of two cu-boxes and prevents the flow of the buffer wind-heat exchangers 7 form a cascade solution from the upper compartment to the bottom 1511659

compartments 1 assembled two electrodes 9 parallel to each other, the plug S of which is led to the side wall of the lower electrode compartment 1, the other two electrodes 11 are mounted in the cap 12 of the upper electrode compartment 2, and their plug 13 is pulled out on the attachment 12. Top electrode compartment 2 mounted on special protrusions 14. For filling the cassettes 6 (FIG. 3) with a gel-forming solution and for sealing the rectangular recess 3

plate 15 with a cone-shaped recess 16 and a hole with a fitting 17. A rubber gasket 18 is fixed along the perimeter of the plate 15. The plate 15 is attached to the bottom of the upper

electrode compartment 2 using spring clips 19.

The device works as follows.

Block gel cassettes, educative

alternating open at the top of the cuvette-heat exchangers 7 with spacers placed between n1-1mi 8, determining the thickness of the gel plate 6, are placed in a rectangular recess 3

the upper electrode compartment 2. The bottom plate is pressed against it. The plate 15 is fixed with the help of chemis 19. The hole with fitting 17 is connected to a container (not indicated) with a gel-forming solution and the bottom is filled at the bottom.

With the solution and below they fill the cassettes 6. If necessary

the gel-forming solution is poured into one of the cassettes 6, from where it is

filling the cassettes with 6 homogeneous gel is used in a simpler way: the opening with fitting 17 is closed and

the gel-forming solution is poured into one of the cassettes 6, from where it is

the grooves 5 of the perforated bottom of the rectangular recess 3 flow into other cassettes.

In this case, the gel-shaped solution also fills the gaps between the walls of the rectangular recess 3 and the heat exchanger cuvettes 7, and this seals the upper electrode compartment

The gel plate 6 is installed. The cuvette heat exchangers 7 are made of flat glass, are rectangular in shape and are open at the top (Fig. 4).

To obtain a uniform fall of the stress and on all the plates of the 6 gel parallel to the block of gel cassettes, near the walls of the lower electrode

her 1

After the gel is polymerized, the plate 15 is detached from the upper electrode compartment 2, the upper electrode compartment 2 is placed in the lower 1, and a buffer is poured into both compartments. At the same time, cuff-heat exchangers 7 in the upper elec- tric open at the top.

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Claims (1)

Claim A device for electrophoresis in vertical gel plates, including the lower electrode compartment and the upper electrode compartment located in it with a rectangular recess, an elastic sealing cuff installed around the perimeter of the upper end of the recess, gaskets for adjusting the thickness of the gel plates and the cover between the gel plate cassette and the buffer provides effective heat transfer from the gel to the buffer. The presence of a perforated bottom in the 'Rectangular recess allows you to work even with thick (5-15 mm) and low (50-60 mm) gel plates of low concentration (5-7%) and eliminates the sliding of gels from the cassettes. , The design of the device is characterizable. 20 ku for sealing gel cassettes from the bottom, characterized in that, in order to simplify the device, it contains rectangular cuvettes, heat exchangers, walls open from above 25 of which are at the same time walls of gel cassettes located at a distance equal to the thickness of the gel plate from each other, and the recess of the upper electrode compartment is made simple, reliable and convenient with a perforated bottom.