SG11201913157RA - Aav vector column purification methods - Google Patents
Aav vector column purification methodsInfo
- Publication number
- SG11201913157RA SG11201913157RA SG11201913157RA SG11201913157RA SG11201913157RA SG 11201913157R A SG11201913157R A SG 11201913157RA SG 11201913157R A SG11201913157R A SG 11201913157RA SG 11201913157R A SG11201913157R A SG 11201913157RA SG 11201913157R A SG11201913157R A SG 11201913157RA
- Authority
- SG
- Singapore
- Prior art keywords
- purification methods
- aav vector
- column purification
- vector column
- aav
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762527633P | 2017-06-30 | 2017-06-30 | |
| US201762531744P | 2017-07-12 | 2017-07-12 | |
| US201762567905P | 2017-10-04 | 2017-10-04 | |
| PCT/US2018/040430 WO2019006390A1 (en) | 2017-06-30 | 2018-06-29 | AAV VECTOR COLUMN PURIFICATION METHOD |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201913157RA true SG11201913157RA (en) | 2020-01-30 |
Family
ID=64742749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201913157RA SG11201913157RA (en) | 2017-06-30 | 2018-06-29 | Aav vector column purification methods |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20210079422A1 (ja) |
| EP (1) | EP3658250A4 (ja) |
| JP (2) | JP2020526190A (ja) |
| KR (2) | KR102669561B1 (ja) |
| CN (1) | CN111032176A (ja) |
| AU (1) | AU2018291023B2 (ja) |
| BR (1) | BR112019028299A2 (ja) |
| CA (1) | CA3068622A1 (ja) |
| CL (1) | CL2019003915A1 (ja) |
| CO (1) | CO2020000911A2 (ja) |
| IL (1) | IL271745A (ja) |
| MX (1) | MX2020000216A (ja) |
| PE (1) | PE20200737A1 (ja) |
| PH (1) | PH12020500044A1 (ja) |
| SG (1) | SG11201913157RA (ja) |
| WO (1) | WO2019006390A1 (ja) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201508026D0 (en) | 2015-05-11 | 2015-06-24 | Ucl Business Plc | Capsid |
| WO2020210600A1 (en) * | 2019-04-11 | 2020-10-15 | Regenxbio Inc. | Methods of size exclusion chromatography for the characterization of recombinant adeno-associated virus compositions |
| EP3919613B1 (en) | 2020-06-05 | 2024-08-07 | Sartorius BIA Separations d.o.o. | Enhanced purification of adeno-associated virus to more effectively remove contaminating dna |
| JP2023537565A (ja) * | 2020-06-24 | 2023-09-04 | バイオベラティブ セラピューティクス インコーポレイテッド | タンパク質を発現するように改変されたレンチウイルスベクターの調製物から遊離第ｖｉｉｉ因子を除去する方法 |
| US20220033782A1 (en) * | 2020-07-29 | 2022-02-03 | Pall Corporation | Adenovirus-associated viruses separation method |
| EP4229205A1 (en) | 2020-10-15 | 2023-08-23 | F. Hoffmann-La Roche AG | Nucleic acid constructs for va rna transcription |
| CN116348607A (zh) | 2020-10-15 | 2023-06-27 | 豪夫迈·罗氏有限公司 | 用于同时基因活化的核酸构建体 |
| JP2022182361A (ja) * | 2021-05-28 | 2022-12-08 | ダイセン・メンブレン・システムズ株式会社 | 微小有用物質の分離精製方法と分離精製装置 |
| JP2022182360A (ja) * | 2021-05-28 | 2022-12-08 | ダイセン・メンブレン・システムズ株式会社 | 細胞外小胞の分離精製方法 |
| MX2023014809A (es) * | 2021-06-11 | 2024-01-29 | Spark Therapeutics Inc | Metodos de purificacion por columna de vectores aav. |
| AU2022358612A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process of purification of fusion protein |
| CN114250222A (zh) * | 2021-12-08 | 2022-03-29 | 苏州博腾生物制药有限公司 | 一种从动物组织中提取aav dna的方法 |
| GB202117844D0 (en) * | 2021-12-09 | 2022-01-26 | Oxford Biomedica Ltd | Purification method |
| JP2023141423A (ja) * | 2022-03-24 | 2023-10-05 | 国立大学法人 東京大学 | ウイルスの精製方法 |
| WO2023198685A1 (en) | 2022-04-13 | 2023-10-19 | F. Hoffmann-La Roche Ag | Method for determining aav genomes |
| WO2023227438A1 (en) | 2022-05-23 | 2023-11-30 | F. Hoffmann-La Roche Ag | Raman-based method for the differentiation of aav particle serotype and aav particle loading status |
| WO2023232922A1 (en) | 2022-06-03 | 2023-12-07 | F. Hoffmann-La Roche Ag | Method for producing recombinant aav particles |
| WO2024013239A1 (en) | 2022-07-14 | 2024-01-18 | F. Hoffmann-La Roche Ag | Method for producing recombinant aav particles |
| WO2024024337A1 (ja) * | 2022-07-28 | 2024-02-01 | 株式会社ダイセル | 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法 |
| WO2024056561A1 (en) | 2022-09-12 | 2024-03-21 | F. Hoffmann-La Roche Ag | Method for separating full and empty aav particles |
| EP4385618A1 (en) | 2022-12-16 | 2024-06-19 | Chiral Technologies Europe SAS | Composite material for bioseparations |
| WO2024165456A1 (en) | 2023-02-07 | 2024-08-15 | F. Hoffmann-La Roche Ag | Method for the detection of anti-aav particle antibodies |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2325299A3 (en) * | 1997-09-05 | 2011-10-05 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of recombinant AAV vectors |
| US6593123B1 (en) * | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| ATE490307T1 (de) * | 2003-05-21 | 2010-12-15 | Genzyme Corp | Verfahren zur herstellung von präparationen rekombinanter aav-virionen, die weitgehend frei von leeren capsiden sind |
| ES2693194T3 (es) * | 2009-06-16 | 2018-12-10 | Genzyme Corporation | Métodos mejorados para la purificación de vectores de AAV recombinantes |
| BR112012018899A2 (pt) * | 2010-01-28 | 2015-09-15 | Philadelphia Children Hospital | "método para purificar partículas de vetor de vírus adeno-associado." |
| PE20240371A1 (es) * | 2015-12-01 | 2024-03-05 | Spark Therapeutics Inc | Metodos escalables para producir un vector viral adenoasociado (aav) recombinante en un sistema de cultivo celular en suspension sin suero adecuado para uso clinico |
| WO2017100676A1 (en) * | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav8 |
| WO2017100674A1 (en) * | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav1 |
| ES2918998T3 (es) * | 2015-12-11 | 2022-07-21 | Univ Pennsylvania | Método de purificación escalable para AAVrh10 |
| PE20190434A1 (es) * | 2016-03-31 | 2019-03-21 | Spark Therapeutics Inc | PROCESO DE PRODUCCION DE rAAV A BASE DE COLUMNAS COMPLETAMENTE ESCALABLE |
-
2018
- 2018-06-29 US US16/627,227 patent/US20210079422A1/en active Pending
- 2018-06-29 JP JP2019572175A patent/JP2020526190A/ja active Pending
- 2018-06-29 AU AU2018291023A patent/AU2018291023B2/en active Active
- 2018-06-29 KR KR1020207002118A patent/KR102669561B1/ko active IP Right Grant
- 2018-06-29 PE PE2019002713A patent/PE20200737A1/es unknown
- 2018-06-29 EP EP18823181.5A patent/EP3658250A4/en active Pending
- 2018-06-29 SG SG11201913157RA patent/SG11201913157RA/en unknown
- 2018-06-29 BR BR112019028299-8A patent/BR112019028299A2/pt unknown
- 2018-06-29 CA CA3068622A patent/CA3068622A1/en active Pending
- 2018-06-29 WO PCT/US2018/040430 patent/WO2019006390A1/en active Application Filing
- 2018-06-29 CN CN201880053245.7A patent/CN111032176A/zh active Pending
- 2018-06-29 KR KR1020247016903A patent/KR20240093848A/ko unknown
- 2018-06-29 MX MX2020000216A patent/MX2020000216A/es unknown
-
2019
- 2019-12-29 IL IL271745A patent/IL271745A/en unknown
- 2019-12-30 CL CL2019003915A patent/CL2019003915A1/es unknown
-
2020
- 2020-01-03 PH PH12020500044A patent/PH12020500044A1/en unknown
- 2020-01-28 CO CONC2020/0000911A patent/CO2020000911A2/es unknown
-
2022
- 2022-11-07 JP JP2022177960A patent/JP2023029832A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| RU2020103743A (ru) | 2021-07-30 |
| AU2018291023B2 (en) | 2023-11-02 |
| EP3658250A4 (en) | 2021-10-27 |
| KR20240093848A (ko) | 2024-06-24 |
| WO2019006390A1 (en) | 2019-01-03 |
| PH12020500044A1 (en) | 2020-09-14 |
| KR20200040749A (ko) | 2020-04-20 |
| CO2020000911A2 (es) | 2020-06-19 |
| JP2023029832A (ja) | 2023-03-07 |
| CA3068622A1 (en) | 2019-01-03 |
| CN111032176A (zh) | 2020-04-17 |
| EP3658250A1 (en) | 2020-06-03 |
| KR102669561B1 (ko) | 2024-05-24 |
| US20210079422A1 (en) | 2021-03-18 |
| JP2020526190A (ja) | 2020-08-31 |
| RU2020103743A3 (ja) | 2021-09-30 |
| AU2018291023A1 (en) | 2020-02-06 |
| BR112019028299A2 (pt) | 2020-07-14 |
| IL271745A (en) | 2020-02-27 |
| PE20200737A1 (es) | 2020-07-23 |
| CL2019003915A1 (es) | 2020-06-19 |
| MX2020000216A (es) | 2020-09-03 |
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