SG11201809996QA - Ribonucleic acid (rna) interactions - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property :::` , 1111111111111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number 03 (43) International Publication Date .....•\"\" WO 2017/196264 Al 16 November 2017 (16.11.2017) WIPO I PCT (51) International Patent Classification: HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, C12Q 1/68 (2006.01) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (21) International Application Number: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PCT/SG2017/050254 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, (22) International Filing Date: TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 12 May 2017 (12.05.2017) (84) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (30) Priority Data: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 10201603786V 12 May 2016 (12.05.2016) SG EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, KM, ML, MR, NE, SN, TD, TG). #20-10 Connexis, Singapore 138632 (SG). (72) Inventors: WAN, Yue; c/o Genome Institute of Singa- Declarations under Rule 4.17: pore, 60, Biopolis Street, Singapore 138672 (SG). AW, — of inventorship (Rule 4.17(iv)) Jong Ghut Ashley; c/o Genome Institute of Singapore, 60, Published: — with Biopolis Street, Singapore 138672 (SG). NAGARAJAN, — international search report (Art. 21(3)) Niranj an; c/o Genome Institute of Singapore, 60, Biopo- — with sequence listing part of description (Rule 5.2(a)) lis Street, Singapore 138672 (SG). WILM, Andreas; c/o = Genome Institute of Singapore, 60, Biopolis Street, Singa- _ pore 138672 (SG). SUN, Miao; c/o Genome Institute of Sin- gapore, 60, Biopolis Street, Singapore 138672 (SG). = Agent: DONALDSON & BURKINSHAW LLP; 24 Raf- fles Place #15-00, Clifford Centre, Singapore 048621 (SG). (74) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, = (81) = = = Title: RIBONUCLEIC ACID (RNA) INTERACTIONS = (54) Figure 1 = = A = Psoralert cross g RNA extraction. Proxirritly ligation klapter ligation, Analysts ot -chimeras in-vivo Fragmentation Reverse cresslinking Rr, PCR, deep = Bead esichinen/ sequencing = Biotin %.„,„ PsorMen` — ' 11 (57) : The invention relates to a method for analysing ribonucleic acid (RNA) interactions comprising : a) cross-linking base- paired nucleotides of at least one RNA molecule and/or at least one pair of RNA molecules using a tagged, reversible cross-linking N agent (preferably tagged-psoralen) under ultraviolet irradiation; b) fragmenting the said cross-linked RNA molecule(s); c) using said tag to extract said cross-linked RNA fragment(s); d) ligating the said cross-linked RNA fragment(s) to produce cross-linked ligated RNA , 1 chimera(s); e) reversing the cross-linking of the said agent to the said RNA molecule(s); f) preparing a sequence library by sequencing .--- - t ,,, the ligated RNA chimera molecule(s) or pair(s); and g) analysing the sequence library to determine RNA interactions. Also disclosed - is a method of studying a subject by analysing RNA interactions and attributing them to a clinical picture, or a drug discovery method 0 by attributing an efficacy score to the drug based upon determined RNA interactions. ei C