SG10201408414RA - Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging - Google Patents

Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging

Info

Publication number
SG10201408414RA
SG10201408414RA SG10201408414RA SG10201408414RA SG10201408414RA SG 10201408414R A SG10201408414R A SG 10201408414RA SG 10201408414R A SG10201408414R A SG 10201408414RA SG 10201408414R A SG10201408414R A SG 10201408414RA SG 10201408414R A SG10201408414R A SG 10201408414RA
Authority
SG
Singapore
Prior art keywords
model
mbp
basic protein
myelin basic
luci
Prior art date
Application number
SG10201408414RA
Inventor
James Cao
Karen Chandross
Kyriakos D Economides
Harry Gregory Polites
Daniel Weinstock
Xiaoyou Ying
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US28737109P priority Critical
Priority to US33463710P priority
Priority to FR1057581 priority
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of SG10201408414RA publication Critical patent/SG10201408414RA/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027New breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/20Animal model comprising regulated expression system
    • A01K2217/206Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/10Mammal
    • A01K2227/105Murine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • A01K2267/0393Animal model comprising a reporter system for screening tests

Abstract

ANIMAL MODEL EXPRESSING LUCIFERASE UNDER CONTROL OF THE MYELIN BASIC PROTEIN PROMOTER (MBP-LUCI) AND USE OF THE MODEL FOR BIOLUMINESCENCE IN VIVO IMAGING A Myelin Basic Protein-luciferase bioimaging noninvasive model to visualize and quantify demyelination and remyelination events in the CNS at transcriptional level in vivo is provided. Luciferase-expressing transgenic animals were generated with myelin basic protein (MBP) promoter coupled to firefly luciferase reporter. The MBP- luci bioimaging model provides a means to monitor myelination status and the efficacy of a remyelination modulating test compound. An advantage of bioimaging is that a subject in a longitudinal study can serve as its own control. The same subject can be tracked over a demyelination and remyelination process continuously over a period of at least 10 weeks. This model enables normalization of individual animal imaging response and provides quality data with considerably reduced variance. In addition, because cohorts of animals need not be sacrificed at different time points, reduction in the number necessary for a compound efficacy study is possible. [Fig. 1]
SG10201408414RA 2009-12-17 2010-12-03 Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging SG10201408414RA (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US28737109P true 2009-12-17 2009-12-17
US33463710P true 2010-05-14 2010-05-14
FR1057581 2010-09-21

Publications (1)

Publication Number Publication Date
SG10201408414RA true SG10201408414RA (en) 2015-01-29

Family

ID=44305684

Family Applications (2)

Application Number Title Priority Date Filing Date
SG2012041141A SG181509A1 (en) 2009-12-17 2010-12-03 Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging
SG10201408414RA SG10201408414RA (en) 2009-12-17 2010-12-03 Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG2012041141A SG181509A1 (en) 2009-12-17 2010-12-03 Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging

Country Status (15)

Country Link
US (2) US20130117868A1 (en)
EP (2) EP3138396A1 (en)
JP (2) JP6068981B2 (en)
KR (1) KR20120107113A (en)
CN (2) CN104975042A (en)
AR (1) AR079537A1 (en)
AU (2) AU2010340238B2 (en)
BR (1) BR112012015876A2 (en)
CA (1) CA2784652A1 (en)
IL (1) IL220244D0 (en)
MX (1) MX2012006938A (en)
RU (2) RU2601128C2 (en)
SG (2) SG181509A1 (en)
TW (1) TW201137354A (en)
WO (1) WO2011084281A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101510252B1 (en) * 2013-11-15 2015-04-10 대한민국 Specific light wavelength BrEXPA1 promoter from brassica rapa and uses thereof
KR20170058977A (en) 2014-09-17 2017-05-29 개리슨 덴탈 솔루션즈, 엘엘씨 Dental curing light
USD810293S1 (en) 2017-01-20 2018-02-13 Garrison Dental Solutions, Llc Dental instrument
RU2674894C2 (en) * 2017-01-30 2018-12-13 Общество с ограниченной ответственностью "ПЛАНТА" New luciferases and methods of their use

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8615942D0 (en) * 1986-06-30 1986-08-06 Animal & Food Research Council Peptide production
US5650135A (en) 1994-07-01 1997-07-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
WO2002051981A2 (en) * 2000-12-13 2002-07-04 Baylor College Of Medecine Defects in periaxin associated with myelinopathies
US7285415B2 (en) * 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
JP2005532814A (en) * 2002-07-17 2005-11-04 ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company The transgenic non-human mammal expressing a reporter nucleic acid under the control of androgen responsive element
JP2004242419A (en) * 2003-02-05 2004-08-26 Sumitomo Heavy Ind Ltd Overload detection device for motor
SG138623A1 (en) 2004-04-01 2008-01-28 Aventis Pharmaceuticals Inc Us Use of ppr delta agonists for treating demyelinating diseases
CA2660321A1 (en) * 2006-08-10 2008-02-21 The Board Of Trustees Of The Leland Stanford Junior University Differential labeling of cells
EP2073833A2 (en) * 2006-10-20 2009-07-01 Biogen Idec MA Inc. Treatment of demyelinating disorders with soluble lymphotoxin-beta-receptor
JP2008125452A (en) * 2006-11-21 2008-06-05 Tohoku Univ Nonhuman mammal of oligodendrocyte developmental disorder model
WO2009023517A2 (en) * 2007-08-10 2009-02-19 Wayne State University Compositions and methods for detecting and modulating cell death by a translation regulated gene expression system

Also Published As

Publication number Publication date
EP2512227B1 (en) 2016-11-02
SG181509A1 (en) 2012-07-30
AU2010340238A1 (en) 2012-07-05
RU2012130092A (en) 2014-01-27
JP6068981B2 (en) 2017-01-25
IL220244D0 (en) 2012-07-31
TW201137354A (en) 2011-11-01
US20160066550A1 (en) 2016-03-10
AU2016247219A1 (en) 2016-11-10
AR079537A1 (en) 2012-02-01
CA2784652A1 (en) 2011-07-14
RU2016138560A (en) 2018-12-13
JP2013514534A (en) 2013-04-25
CN102770020B (en) 2015-07-15
EP2512227A1 (en) 2012-10-24
RU2016138560A3 (en) 2018-12-13
CN104975042A (en) 2015-10-14
CN102770020A (en) 2012-11-07
WO2011084281A1 (en) 2011-07-14
BR112012015876A2 (en) 2016-11-16
MX2012006938A (en) 2012-07-10
US20130117868A1 (en) 2013-05-09
EP3138396A1 (en) 2017-03-08
AU2010340238B2 (en) 2016-07-21
JP2017102120A (en) 2017-06-08
RU2601128C2 (en) 2016-10-27
KR20120107113A (en) 2012-09-28

Similar Documents

Publication Publication Date Title
Callis et al. MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice
Xu et al. GDF15/MIC-1 functions as a protective and antihypertrophic factor released from the myocardium in association with SMAD protein activation
Berry et al. Weighing in on adipocyte precursors
Panksepp et al. Affiliative behavior, ultrasonic communication and social reward are influenced by genetic variation in adolescent mice
Liu et al. Age-dependent in vivo conversion of mouse cochlear pillar and Deiters' cells to immature hair cells by Atoh1 ectopic expression
Li et al. Mouse embryonic head as a site for hematopoietic stem cell development
Grossman et al. Characterization of behavioral and endocrine effects of LSD on zebrafish
Van Keymeulen et al. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis
Eckardt et al. Functional role of connexin43 gap junction channels in adult mouse heart assessed by inducible gene deletion
Shao et al. Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract
Knapp et al. Loss of myogenin in postnatal life leads to normal skeletal muscle but reduced body size
Dymecki et al. Molecular neuroanatomy's “Three Gs”: a primer
Robertson et al. Developmental origins of central norepinephrine neuron diversity
Kishi et al. Common marmoset as a new model animal for neuroscience research and genome editing technology
Ono et al. Inhibition of class IIb histone deacetylase significantly improves cloning efficiency in mice
Groves et al. Ablating adult neurogenesis in the rat has no effect on spatial processing: evidence from a novel pharmacogenetic model
Bielsky et al. Sexual dimorphism in the vasopressin system: lack of an altered behavioral phenotype in female V1a receptor knockout mice
Perl et al. Conditional expression of genes in the respiratory epithelium in transgenic mice: cautionary notes and toward building a better mouse trap
Serón-Ferré et al. Circadian rhythms in the fetus
Xin et al. Smooth muscle expression of Cre recombinase and eGFP in transgenic mice
Kimura et al. Impairments in remote memory stabilization precede hippocampal synaptic and cognitive failures in 5XFAD Alzheimer mouse model
EA025172B9 (en) Method for determining developmental potential of one or more human embryos to reach blastocyst stage
Qiu et al. Circuit-specific intracortical hyperconnectivity in mice with deletion of the autism-associated Met receptor tyrosine kinase
MX349622B (en) Methods for assessing and identifying or evolving conditionally active therapeutic proteins.
Pippin et al. Cells of renin lineage are progenitors of podocytes and parietal epithelial cells in experimental glomerular disease