SE8500622D0 - GAS BREAKING CELL CULTIVATION - Google Patents

GAS BREAKING CELL CULTIVATION

Info

Publication number
SE8500622D0
SE8500622D0 SE8500622A SE8500622A SE8500622D0 SE 8500622 D0 SE8500622 D0 SE 8500622D0 SE 8500622 A SE8500622 A SE 8500622A SE 8500622 A SE8500622 A SE 8500622A SE 8500622 D0 SE8500622 D0 SE 8500622D0
Authority
SE
Sweden
Prior art keywords
cells
gas
sparging
cell
medium
Prior art date
Application number
SE8500622A
Other languages
Swedish (sv)
Other versions
SE8500622L (en
Inventor
R G Rupp
Original Assignee
Damon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Damon Corp filed Critical Damon Corp
Publication of SE8500622D0 publication Critical patent/SE8500622D0/en
Publication of SE8500622L publication Critical patent/SE8500622L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers

Landscapes

  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of growing fragile cells, i.e., cells having no cell membrane such as animal cells or genetically modified animal cells, to high density in culture comprises the steps of suspending permeable microcapsules containing a seed culture of such cells in a medium, directly sparging a gas through the medium along the capsules, and allowing the cells to grow. The gas comprises a gas or a selected mixture of gases and is sparged at a rate high enough to attain a substantial partial pressure equilibrium between the gas and liquid phase. The partial pressure of gases essential for cell growth and normal rates of cell mitosis can be maintained in the medium by controlling the composition of the gas and maintaining a threshold sparging level. By sparging, oxygen availability need no longer be a growth-rate limiting factor, and cell densities on the order of 10<8> cells/ml may be achieved.
SE8500622A 1984-02-13 1985-02-12 GAS BREAKING CELL CULTIVATION SE8500622L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57949384A 1984-02-13 1984-02-13

Publications (2)

Publication Number Publication Date
SE8500622D0 true SE8500622D0 (en) 1985-02-12
SE8500622L SE8500622L (en) 1985-08-14

Family

ID=24317121

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8500622A SE8500622L (en) 1984-02-13 1985-02-12 GAS BREAKING CELL CULTIVATION

Country Status (11)

Country Link
JP (1) JPS60199380A (en)
AU (1) AU572608B2 (en)
BE (1) BE901702A (en)
DE (1) DE3504748A1 (en)
DK (1) DK65085A (en)
FR (1) FR2559500A1 (en)
GB (1) GB2154246B (en)
IT (1) IT1184884B (en)
NL (1) NL8500351A (en)
NO (1) NO850534L (en)
SE (1) SE8500622L (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media
US4829002A (en) * 1986-05-12 1989-05-09 Baxter International Inc. System for metering nutrient media to cell culture containers and method
WO1988000967A1 (en) * 1986-08-04 1988-02-11 The University Of New South Wales Serum free tissue culture medium containing polymeric cell-protective agent
JPS6423888A (en) * 1987-07-16 1989-01-26 Etsuko Kakizaki Culture vessel with micro-cellular wall
JP2509630B2 (en) * 1987-07-29 1996-06-26 三井石油化学工業株式会社 Culture method and culture device
DE3739650C1 (en) * 1987-11-23 1989-05-24 Immuno Ag Fermenter for growing cell cultures
WO2005035748A1 (en) 2003-10-10 2005-04-21 Novo Nordisk Health Care Ag Method for large-scale production of a polypeptide in eukaryote cells and a culture vessel suitable therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4409331A (en) * 1979-03-28 1983-10-11 Damon Corporation Preparation of substances with encapsulated cells
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media

Also Published As

Publication number Publication date
SE8500622L (en) 1985-08-14
IT1184884B (en) 1987-10-28
DE3504748C2 (en) 1987-07-23
IT8567141A1 (en) 1986-08-12
FR2559500A1 (en) 1985-08-16
DE3504748A1 (en) 1985-09-05
JPS6110115B2 (en) 1986-03-28
GB2154246B (en) 1987-09-09
NL8500351A (en) 1985-09-02
GB8503248D0 (en) 1985-03-13
BE901702A (en) 1985-05-29
DK65085D0 (en) 1985-02-12
DK65085A (en) 1985-08-14
AU3857185A (en) 1985-08-22
JPS60199380A (en) 1985-10-08
IT8567141A0 (en) 1985-02-12
NO850534L (en) 1985-08-14
AU572608B2 (en) 1988-05-12
GB2154246A (en) 1985-09-04

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Legal Events

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NAV Patent application has lapsed

Ref document number: 8500622-9

Effective date: 19890901