RU2798579C1 - Medicinal product for treatment of skin diseases of animals - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: medicinal product for the treatment of skin diseases of cows is characterized by the following: it includes a coniferous-glycerin dietary supplement, pine tar, isopropyl alcohol, petroleum jelly in the following ratio — 20:30:10:40 used in veterinary dermatology once every 2–3 days at a dose of 30 ml 5–7 times.

EFFECT: invention allows to develop a new medicinal product formulation for the treatment of animal skin diseases with increased homogeneity.

1 cl, 4 tbl, 2 ex

Description

The invention relates to veterinary medicine, in particular, to a method of using a veterinary ointment for the treatment of skin diseases, burns, cracks, damage to the skin and tissues of animals.

The intensive development of animal husbandry, along with numerous positive aspects, has some significant drawbacks, the main of which is the concentration of a large number of animals in a relatively small area and non-compliance with zoohygienic norms and rules for keeping animals, which is often noted in livestock farms.

The above situation in livestock complexes dictates the need to develop new types of highly effective, inexpensive and harmless to animals ointments that would be effective, environmentally friendly (would consist of natural components), economical and satisfy the needs of intensive animal husbandry.

At the moment, in veterinary dermatology, the most in demand are products that include various antibiotics, antiseptics, vitamins, hormones, etc. These include such well-known drugs as Vishnevsky liniment, levomekol, mastiet-forte, zinc ointment, sulfuric ointment, etc.

A known method for improving the effectiveness of the treatment of atopic dermatitis with glucocorticosteroid ointments [Patent RU 2170092, publ. 07/10/2001 (1)].

The method consists in local use of a glucocorticosteroid ointment additionally containing heparin on the lesions, while the dose of steroids is reduced by 13-40 times. The method leads to an increase in the effect of treatment and a decrease in the complications of steroid therapy. However, in veterinary dermatology, due to the high cost of the drug, this method of distribution has not received.

Known drug for the treatment of purulent wounds in animals [Patent RU 2473328, publ. 01/27/2013, BI No. 3 (2)], including the active substance and the ointment base - polyethylene oxide-1500 and polyethyl oxide-400, characterized in that the active substance is a product of dry sublimation of birch fungus chaga, mixed with polyethylene oxide-400 and polyethylene oxide-1500 in a ratio of 1.25:4:1, respectively, at a temperature of 39-41°C. However, this drug includes potent chemical antiseptics, which have contraindications for use.

There is an ointment for the treatment of purulent wounds in farm animals [Patent RU 2035183, publ. 05/20/1995 (3)], which includes copper sulfate. It promotes rapid healing of wounds.

Known methods of preparing ointments, including mixing medicinal or biologically active substances with a fatty base of the ointment [Handbook ed. I.E. Mozgov. Veterinary formulation with the basics of therapy and prevention. M. VO "Agropromizdat", 1988 (4)].

The composition of the anti-inflammatory ointment for external use "Pichtoin" is known, including pine resin 19.0-21.0%, beeswax 16.8-17.5%, chalk 10.5-12.0%, the rest fat base [Patent RU 2097056 , publ. 1997.11.27(5)]. However, the method of its preparation is laborious and ineffective, the presence of chalk negatively affects the technology of preparation and application of an ointment based on it.

In medicine, for the treatment of skin inflammation, acne is widely used ichthyol ointment (prototype). It has an antimicrobial and antibacterial effect, improves the outflow of fluid, accelerates the maturation of the boil, and is effective for erysipelas and eczema.

The aim of the present invention is to develop a new formulation and recommendations for the use of an ointment for the treatment of skin diseases in animals, which makes it possible to increase the homogeneity, the main quality of the resulting ointment.

The drug for the treatment of skin diseases of cows, is characterized by the fact that it includes a coniferous-glycerin dietary supplement, pine tar, isopropyl alcohol, petroleum jelly in the following ratio - 20:30:10:40, which is used in veterinary dermatology once every 2-3 days at a dose of 30 ml 5-7 times.

Coniferous-glycerin dietary supplement - glycerin extract of woody greens of Scots pine [RF Patent 2579494, Publ. 04/10/2016. Bull. No. 10 (6)] regulates metabolic processes in the body of animals, supports the normal functioning of the enzymatic system of the body, ensures the immunobiological activity of the body. The composition and preparation technology was developed by LLC NTC "Khiminvest", Nizhny Novgorod. The glycerin included in the additive has antibacterial properties, has a softening effect on the skin, and also ensures the safety of the consumer qualities of the product for a long time under normal storage conditions.

Pine tar [RF Patent 2578695, Publ. 03/27/2016. Bull. No. 9 (7)] - a product of dry distillation of coniferous wood. Tar is used as a disinfectant, insecticide for the treatment of skin diseases (eczema, lichen, scabies, etc.) as part of many ointments widely used in medicine. Due to the content of phenols and resins, tar has a strong restorative, antiseptic, antiparasitic, drying, anti-inflammatory, anesthetic effect; reduces erythema, soothes itching. In chronic allergic dermatoses and some other infiltrative processes in the skin, tar has a resolving effect, reduces skin hyperemia.

Isopropyl alcohol is a colorless liquid with a strong alcohol odor. Isopropanol dissolves well natural and some synthetic resins, ethyl cellulose, polyvinyl butyral, most oils, an excellent disinfectant used in medicine and veterinary medicine. In developed countries, isopropanol is widely used in personal care products and medicine due to its relatively low toxicity.

Veterinary Vaseline is a highly refined product obtained by fusing ceresin, paraffin, petralatum and petroleum oils. The drug is a homogeneous ointment without lumps from white to light brown. It has a softening effect, compensating for an insufficient amount of fatty lubrication, softening dead skin cells. In addition, covering the skin with an even layer, it protects extrareceptors from external influences. Vaseline veterinary can be used in combination with any drugs in the manufacture of creams and ointments. Contraindications, side effects and complications in the use of veterinary vaseline have not been established.

Example 1. 40 kg of veterinary vaseline is loaded into a reactor equipped with a stirrer, melted at a temperature of 40-50 ° C, 20 kg of coniferous-glycerin biologically active are added, thoroughly mixed for 20 minutes until a homogeneous consistency, then, with constant stirring, portions are served 30 kg of pine tar and 10 kg of isopropyl alcohol mix the ointment for 10-20 minutes until a homogeneous consistency, then cool and package.

The resulting ointment is a homogeneous viscous mass of dark brown color with a recognizable smell of tar, a homogeneous consistency.

Example 2. Studies were carried out on the basis of JSC "Zarya" Vologda district of the Vologda region on cows of black-motley Holstein breed with a milk yield of about 8000 kg of milk per lactation. The maintenance of animals is stall tied. The studies were carried out in the winter-spring-summer period of 2022.

At the first stage, the cows were examined for the presence of skin lesions. All examined animals were divided into 2 groups: experimental (12 heads) and control group (7 heads).

Animals of the experimental group were treated with the tested volatile ointment, cows of the control group with wounds were treated with the application of ichthyol ointment.

Treatment of the experimental group of animals was carried out until the formation of a "crust of tension" and drying of the skin after 48 and 72 hours. At the same time, on average, 30 ml of the product was used per treatment.

Ichthyol ointment was applied to the affected area of the skin of the control group of animals in the amount of 40 g.

Experimental cows are mainly animals of 1-6 lactations. The average number of lactations in the groups was approximately equal to 2.5-3 lactations. The productivity of experimental cows for the previous lactation was in the range of 7300-9600 kg of milk.

As a result of general clinical studies (measurement of temperature, pulse, respiration and rumination), as well as examination of animals and determination of the general condition, no deviations from the norm were detected both before and after treatment. So, on average, the body temperature of cows ranged from 37.8°C to 38.9°C. Pulse - 56-72 beats / min. Breathing - 18-24 times / min. Rumination - 2-5 in 5 minutes. All indicators were within the normal range.

In cows of all groups, blood samples were taken from the subcaudal vein with vacuum tubes for hematological analysis before and after the experiment. The results of the analysis are presented in tables 2, 3.

As can be seen from Table 2, before treatment, the average hematological parameters of the blood of experimental cows, basically, corresponded to the norm. The exception is low leukocytes in the cow Nadezhda in the experimental group, which may mean there are problems with an imbalance in the functioning of the kidneys or a bacterial or viral infection, as well as the use of bacterial preparations. Segmented and stab neutrophils in general in groups correspond to the norm, with the exception of single cows in each experimental group, which can be explained by the individual state of the body associated with a different physiological state (presence of pregnancy or early lactation).

The general condition of animals of all groups is satisfactory. In some experimental cows, increased activity is observed, which is expressed in an attempt to lick (scratch) the back of the body or regular movement of the tail, which is explained by the discomfort associated with damage to the skin

As for the blood results after treatment, it can be noted that leukocytes and eosinophils in the cows of the experimental group returned to normal. Monocytes in the control group slightly increased, while in the cows of the experimental group they decreased, but still exceed the upper limits of the norm. In general, in all experimental groups, blood counts improved.

The experimental group of cows and 7 heads of the control group were also treated for wounds, eczema, dermatitis with different means (Table 1). The test preparation was applied to cows after 48 hours (6 heads) and after 72 hours (6 heads). Ichthyol ointment was applied to animals of the control group (7 heads) daily 1 time per day.

The results of the treatment of the experimental group: in six cows with the application of the product, after 48 hours, a “drying crust” began to form after the fifth treatment, i.e. after 10 days. In cows treated 72 hours after 6-8 treatments, i.e. on day 22 on average.

The results of treatment of the control group with ichthyol ointment: wound healing and the formation of a "drying crust" were observed on days 5-9, on average - on days 7.

Thus, in terms of therapeutic efficacy, all compared drugs and methods of treatment were effective.

In accordance with the objectives of the experiment, the indicators of the quantity and quality of milk production of experimental cows were controlled.

Milk productivity in cows of the experimental group increased by 1.3 kg, protein - by 0.64%, somatic cells increased by 99.

Cows with wounds, treated with ichthyol ointment, milked less by 1.1 kg with a slight plus in fat and protein and with a minus of somatic cells.

Treatment costs for experimental cows are presented in Table 4.

Comparative evaluation of the cost-effectiveness of the treatment of wounds, eczema and other dermatitis caused by non-infectious etiology showed the advantage of phytoncidal ointment based on pine tar compared to traditional treatment with ichthyol ointment. The difference is 105-63 rubles. It should be noted that the recovery of the control group came earlier - on the 7th day - compared to the experimental group - on the 10th and 20th day. Although, this did not affect the health and production in a negative way.

Phytoncidal ointment based on pine tar is effective in monotherapy in the treatment of skin wounds, eczema, dermatitis of non-contagious etiology. The ointment is well absorbed into the skin. When applied to the skin, the ointment penetrates through the sebaceous glands and partially through the intercellular passages, where it becomes soluble and gradually absorbed, contributing to the expansion of blood vessels, improving blood supply and accelerating the resorption of hematomas.

Studies have shown that the ointment of the proposed composition provides optimal conditions for the flow of metabolic processes in tissues by influencing local microcirculation, stimulating reparative processes in the wound, as well as preventing its secondary infection, especially with microorganisms that have polyresistance to modern antiseptics.

Treatment of wounds, eczema and other dermatitis of the skin of cows caused by non-contagious etiology is carried out using an ointment based on pine tar once a week at a dose of 30 ml for 7-8 days in a row.

The use of this product does not adversely affect the health of cows and milk production.

The use of phytoncidal ointment based on pine tar is more cost-effective than traditionally used in production products, such as ichthyol ointment - by 42-105 rubles per head. depending on the multiplicity.

Sources of information accepted during the examination of the application:

1. Patent RU 2170092, Publ. 07/10/2001.

2. Patent RU 2473328, Publ. 01/27/2013, BI No. 3.

3. Patent RU 2035183, Publ. May 20, 1995.

4. Handbook, ed. I.E. Mozgov. Veterinary formulation with the basics of therapy and prevention. M. VO "Agropromizdat", 1988

5. Patent RU 2097056, Publ. 1997.11.27.

6. RF patent 2579494, Publ. 04/10/2016. Bull. No. 10.

7. RF patent 2578695, Publ. 03/27/2016. Bull. #9

Claims (1)

A drug for the treatment of skin diseases of cows, characterized in that it includes a coniferous-glycerin dietary supplement, pine tar, isopropyl alcohol, petroleum jelly in the following ratio - 20:30:10:40, which is used in veterinary dermatology once every 2-3 days at a dose of 30 ml 5-7 times.