RU2533154C1 - Complex probiotic feed additive for increase in slaughter weight while growing broiler chickens - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: probiotic feed additive consists of skimmed cow milk fermented with complex of pure cultures of lactose-fermenting microorganisms Enterococcus hirae RNCIM "B-9069", Enterococcus faecium RNCIM "B-9070" and Enterococcus faecium RNCIM "B-9071" in a ratio of 1:1:1.

EFFECT: increase in slaughter weight of broiler chickens.

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Description

The invention relates to biotechnology and can be used to obtain a feed additive for growing broiler chickens.

The purpose of the invention is to obtain a valuable feed supplement based on the use of a complex of local strains of lactic acid bacteria in a ratio of 1: 1: 1 (Enterococcus hirae VKPM B-9069, Enterococcus faecium VKPM B-9070 and Enterococcus faecium VKPM B-9071) with the aim of adding to the main diet feeding when growing broiler chickens.

A known strain of enterococcal bacteria Enterococcus faecium L-3 ND-79 VNIISKHM intended for the manufacture of food products for preventive purposes (RF Patent No. 2220199 C1, publ. 12/27/2003), as well as a method of stimulating the growth of poultry and drug the basis of the probiotic (Patent 2093571 C1, publ. 20.10.1997).

One of the drawbacks, in our opinion, is its low antibiotic activity, as well as the presence of fatty acid microspheres of viable bacteria of the Enterococcus faecium 301, ATCC N 55519 strains formed using a rotor disk.

Also known is a method of obtaining a feed additive to the main diet of broiler chickens using strains of lactobacilli breeding research institute of biotechnology Gorsky GAU. To obtain a probiotic supplement, GOST 10970-87 skimmed milk powder and sourdough are used, consisting of strains of lactic acid microorganisms of the selection research institute of biotechnology Gorsky GAU Enterococcus hirae VPKM B-9069, Enterococcus faecium VKPM B-9070 and Enterococcus faecium VKPM B-9071. Izvestia Gorsky GAU. Part 1. - Volume 47. - Vladikavkaz, 2010. - S.169-170.

The disadvantages of this example can be attributed to the use of milk powder, which must be restored by adding water, which leads to a loss of time and certain energy costs, since milk needs to be heated and sterilized.

To achieve our goal, we used ready-made non-fat cow milk and local yeast strains and the main daily ration of broiler chickens during 3 stages of feeding.

When using a complex of GAU strains Enterococcus hirae VKPM B-9069, Enterococcus faecium VKPM B-9070 and Enterococcus faecium VKPM B-9071, the milk is fermented in milk with a fat content of 0.5% at a temperature optimal for growing, 30-36 ° C , with the introduction of starter culture in the amount of 5% of the volume of the resulting product.

Strains of microorganisms for the fermentation of milk are introduced in a ratio of 1: 1: 1, because all of these strains have different antibiotic properties, while possessing very similar biological and technological properties. In particular, the number of microorganisms in 1 ml of the finished product is the same, and the time required for coagulation of milk is similar.

The productivity of each strain was studied during the experiment when feeding chickens with the addition of milk, fermented with strains of GAU Enterococcus hirae VKPM B-9069, Enterococcus faecium VKPM B-9070 and Enterococcus faecium VKPM B-9071 in the ratio 1: 1: 1, in the amount of 12.5 % of the total diet of the bird. The composition of the fermented milk is given in table 1.

Table 1 The composition of milk fermented with strains Indicators Content Dry matter content,% 9.93 Content,% CB 0.13 Clot formation time, h 6 Ultimate acid-forming ability, ° T 143.4 Acidity at the moment of clot formation, ° Т 77.73

The experiment lasted for 8 weeks.

Example 1. The effectiveness of using a producer strain of probiotic substances in the diet of feeding chickens was studied in the following experimental conditions: feeding time - 56 days, the amount of leaven 12.5% in relation to the main diet. The results of the experiment are given in table.2.

table 2 The results of the use of strains Indicators Content The safety of livestock at the end of the experiment,% 93 Slaughter yield,% 67.51 The composition of the meat of broiler chickens Dry matter% 27.0 Protein,% CB 20.7 Fat,% CB 5.24 Minerals,% CB 1,1

The proposed method for producing a probiotic feed additive allows to obtain a product with a set of useful properties, as well as to exclude time-consuming and technological steps in its production, for example, the restoration of skimmed milk powder or the production of fatty acid microspheres formed with the participation of a rotor disk.

Claims (1)

A probiotic feed additive consisting of skimmed cow's milk, fermented with a complex of pure cultures of lactose-protecting microorganisms Enterococcus hirae VKPM B-9069, Enterococcus faecium VKPM B-9070 and Enterococcus faecium VKPM B-9071 in a 1: 1: 1 ratio.