RU2459865C2 - Growth-stimulating agent for culturing sulphate-reducing bacteria - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: disclosed is a growth-stimulating agent for culturing sulphate-reducing bacteria, which is 2-(furyl-2)-1,3-dioxacyclopentane.

EFFECT: agent enables to increase output of biogenous hydrogen sulphide which is released by sulphate-reducing bacteria and is used to precipitate heavy metals from sewage.

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Description

The invention relates to the creation of new growth stimulators from a number of acetals and their hetero analogs, namely to compounds of the class of furyl-substituted 1,3-diheterocycloalkanes that can be used in the biological treatment of wastewater using sulfate-reducing bacteria.

It is known to use cyclic furfural acetals as plant growth regulators [RF Patent No. 2141202 Plant Growth Regulator “Fetil", BI - 1999. - No. 32].

Information on the use of these compounds as growth regulators of microorganisms in the literature was not found.

It is known to use an emulsion composition of the following composition (wt.%): Nekal 22-30, polyvinyl alcohol 18-26, hydroquinone 12-14, water - the rest as a stimulator of growth and development of SVB in the amount of 0.05-0.2 wt.% [Auth. testimonial. No. 1231812 Method for biochemical treatment of wastewater from metal ions, publ. July 27, 2000].

It is known to use, in order to intensify the sulfate reduction process in the cultivation of SVB, a stimulating additive containing a mixture of double samarium nitrate with pyridine in an amount of 0.0005-0.01 wt.% [Auth. testimonial. No. 152261 A method for detecting sulfate-reducing bacteria in oilfield environments, publ. November 20, 1999].

The disadvantage of these methods is the inaccessibility of the reagents, the complexity of their dosage and the lack of growth-promoting activity of the compounds.

The closest in structure to the stimulators of the growth of SVB (prototype), used in concentrations of 0.0025-0.1 wt.%, Are representatives of the class of nitrogen-containing heteroanalogues of acetals (amines) N, N, N'N'-tetraalkylmethylene (bis) amines of the general formula (1) where R = CH ₃ , C ₂ H ₅ [Auth. testimonial. No. 764388 Nutrient medium for the cultivation of sulfate-reducing bacteria, publ. November 20, 1999]:

The disadvantage is the lack of growth-promoting activity of the compounds.

The invention solves the technical problem of intensifying the production of sulfate-reducing bacteria (SBA) of biogenic hydrogen sulfide used for the deposition of heavy metals in the biological wastewater treatment.

The essence of the invention is to provide a growth-promoting agent for SVB containing, as the active ingredient, 2- (furyl-2) -1,3-dioxacyclopentane:

The preparation of 2- (furyl-2) -1,3-dioxacyclopentane is illustrated by the following examples:

Example 1. Synthesis of 2- (furyl-2) -1,3-dioxacyclopentane under mild conditions (at room temperature):

62 g (1 mol) of freshly distilled ethylene glycol are placed in a beaker and 96 g (1 mol) of freshly distilled furfural is distilled with water by azeotropic distillation with stirring. The mixture is cooled in a water bath to room temperature, then 10 cm ^{3 of} 10% sulfuric acid solution are poured. Stirring is continued for another 0.1 ... 0.15 h. At the time of turbidity of the reaction mixture, indicating the passage of acetalization, 200 cm ^{3 of} water are added to it, after which the reaction mixture is stratified into aqueous and organic phases. After neutralization of the reaction mixture, it was separated using a separatory funnel, the aqueous layer was extracted with ether, the ether extracts and the organic layer were combined, dried over anhydrous CPC, the ether was distilled off and 2- (furyl-2) -1,3-dioxacyclopentane was isolated by vacuum distillation in 84 %

Mp. ° C (mmHg): 105 (18);

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IR spectrum (ν, cm ^-1 ): 3120, 2970, 2860, 1620, 1520, 1475, 1435, 1380, 1245, 1160, 1110, 1030, 960, 930, 890, 870, 805, 750

Example 2. Synthesis of 2- (furyl-2) -1,3-dioxacyclopentane under severe conditions (in boiling benzene):

62 g (1 mol) of freshly distilled ethylene glycol 96 g (1 mol) of freshly distilled furfural and 500 cm ^{3 of} benzene absolute above sodium are placed in a three-necked round-bottom flask equipped with a stirrer, a Dean-Stark trap with a reflux condenser and an inert gas supply. KU-2 catalyst in acid form is added to the cold reaction mixture in an amount of 10 mol% of furfural. The reaction mixture is boiled with stirring for 2-2.5 hours with azeotropic distillation of the reaction water. At the end of the reaction, as evidenced by the cessation of water evolution, the hot mixture is filtered, the catalyst is washed with a small amount of benzene and 2- (furyl-2) -1,3-dioxacyclopentane is isolated by vacuum distillation in 76% yield. The physicochemical characteristics of 2- (furyl-2) -1,3-dioxacyclopeptan are identical to those of the compound obtained above.

Example 3. Laboratory biological tests of the claimed growth stimulating agent.

An experiment to study the effect of organic stimulants on the effectiveness of biochemical sulfate reduction and the development of SVB was performed by incubating microorganisms in penicillin vials (volume 275 ml) for 35 days. At the beginning of the study, an active consortium of SVB containing an accumulative culture of SVB was loaded into sealed glass containers of the same volume.

The sludge load was 1/3 of the bottle height. An identical load was added to all bottles (2 parallel experiments were carried out), filled with nutrient medium of the same composition, adding a growth stimulant in amounts of 0.01-0.1% and a prototype in an amount of 0.05 wt.%. As a control sample, a sample was taken without the addition of a growth stimulant. The dose of the stimulator and prototype was selected on the basis of published data, by analogy with other studies of the growth-regulating activity of 1,3-diheterocycloalkanes [Kalashnikova VG, Mikhaylyuchenko NG, Kulnevich VG Derivatives of 2-furyl-1,3-dioxane are effective stimulants for the growth of nightshade crops. - In: Chemistry and technology of acetals and their heteroanalogs. - Ufa: 1981. - S.139].

As a nutrient medium, we used the medium for selective cultivation of the DSM-63 SVB of the following composition (g / l): glycerol - 1.0, KH ₂ PO ₄ - 0.5, NH ₄ Cl - 1.0, Na ₂ SO ₄ , - 1.0, CaCl ₂ · 2H ₂ O - 0.1, MgSO ₄ · 7H ₂ O - 2.0, yeast extract - 0.1.

Table 1 shows the dynamics of sulfate utilization and the accumulation of hydrogen sulfide observed during the experiment.

The data presented in table 1 show that the claimed compound in concentrations of 0.01-0.1% exhibits a growth-promoting effect during the cultivation of SFS, expressed in the intensification of the processes of utilization of sulfates and the generation of hydrogen sulfide. According to the degree of effectiveness, the claimed growth-promoting substance is better than the prototype taken.

Claims (1)

Growth-promoting agent for the cultivation of sulfate-reducing bacteria, which is 2- (furyl-2) -1,3-dioxacyclopentane.