RU2448721C2 - Method for preparing shelf fungus extract - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical, food and cosmetic industry, particularly to a method for preparing a shelf fungus extract. The method for preparing a shelf fungus extract involving extraction of grinded shelf fungus in 7.5% dimethylsulphoxide, filtration of the whole extract to produce a first stage extract and marc, extraction of marc to produce a second stage extract and combination of the extracts.

EFFECT: shelf fungus extract prepared by the method described above shows high antioxidant activity.

1 tbl, 1 ex

Description

The invention relates to the pharmaceutical, food and cosmetic industries and for the production of chaga extracts.

The prototype is a method for producing water extracts of chaga (RF patent No. 2343930, IPC ⁷ А61К 36/06, B01D 11/02, authors Sysoeva MA, Khabibrahmanova VR, Gamayurova BC, Kuznetsova O.Yu.).

The disadvantage is low antioxidant activity.

The task of the invention is to increase antioxidant activity.

The objective is that in the method for producing chaga extracts, including extracting chopped chaga under certain conditions, filtering off the entire extract to obtain a first extraction step and pulp under certain conditions, extracting the pulp to obtain an extract of a second extraction step and combining the extracts, according to the invention, the chopped chaga is extracted aqueous 7.5% solution of dimethyl sulfoxide.

A method of obtaining extracts of chaga is as follows.

The ground chaga is extracted with an aqueous 7.5% solution of dimethyl sulfoxide. First get the extract of the first stage of extraction, and then the extract of the second stage of extraction, they are combined. At the first and second stages of extraction, a constant temperature of 60-70 ° C is maintained in the extractor. According to the invention, in the first extraction step, the extract is filtered, an extract of the first extraction step and pulp are obtained. An extractant is added to this pulp at the second extraction stage, it is extracted and an extract of the second extraction stage and pulp are obtained (the pulp is removed by filtration). The extracts of the first and second stages of extraction are combined.

For experimental verification of the obtained extracts of chaga were obtained samples described in example 1.

Example 1. 10 g of chopped chaga with a particle diameter of 2-7 mm are placed in an extractor, 60 ml of extractant (aqueous 7.5% solution of dimethyl sulfoxide) are poured and extraction is carried out in an thermostat for 5 hours at a temperature of 70 ° C. After that, the extract is separated from the pulp by filtration, obtaining the extract from the first stage of extraction. The remaining pulp is poured with a new portion of extractant (an aqueous 7.5% solution of dimethyl sulfoxide), in an amount of 40 ml, and extracted for 5 hours in a thermostat at a temperature of 70 ° C. The extract is separated from the pulp by filtration, obtaining the extract from the second stage of extraction. The extracts obtained in the first and second stages of extraction are combined. The yield of polyphenols is 14.13 ± 0.08% by weight of the raw material. The dry residue is 1.80 ± 0.05 g, the ash content of the extract is 13.33 ± 0.06% of the dry residue. The antioxidant activity of the extract is 7.97 ± 0.07 C / ml.

The results of multiple experiments were calculated using the "Statistics 6" program, at P = 0.95, n = 9.

The use of an aqueous solution of dimethyl sulfoxide as an extractant to obtain an extract of chaga DMSO concentration Polyphenol yield Dry residue Ash content Antioxidant activity of the extract % % * g % ** Cl / ml 0 11.71 ± 0.05 1.51 ± 0.22 15.54 ± 0.5 4.21 ± 0.05 7 14.11 ± 0.06 1,60 ± 0,17 14.53 ± 0.7 7.37 ± 1.10 7.5 14.13 ± 0.01 1.80 ± 0.25 13.33 ± 0.5 7.97 ± 0.07 8 14.10 ± 0.08 1.57 ± 0.12 15.52 ± 0.8 7.41 ± 2.20 Note: DMSO - dimethyl sulfoxide, * - percentage of raw materials, ** - percentage of solids

The optimal concentration of the introduction of dimethyl sulfoxide in the extractant is determined - an aqueous 7.5% solution of dimethyl sulfoxide. Using this concentration of dimethyl sulfoxide in the extractant allows you to:

- increase the yield of polyphenols by 2.43%, reduce the ash content of the extract 2.21% and increase its antioxidant activity by 3.76 C / ml.

From the data shown in example 1, it follows that the use of an aqueous solution of dimethyl sulfoxide as an extractant in this invention leads to a twofold increase in the antioxidant activity of the chaga extract obtained according to the invention with respect to the chaga extract obtained by the prototype.

The main active ingredient in water extracts of chaga are chaga polyphenols, the presence of which ensures the therapeutic effectiveness of chaga extracts, therefore, an increase in their yield is an important indicator. The decrease in ash components in the extract, as previously noted in the literature [Andreeva S.I. On the issue of desolation of diffusion juices from chaga. / S.I. Andreeva, P.A. Yakimov, E.V. Alekseeva. // A comprehensive study of physiologically active substances of lower plants: Sat. scientific tr / - M.L .: Nauka, 1961. - P.120-128], also leads to a positive therapeutic effect of drugs based on chaga extracts. The increase in antioxidant activity is one of the indicators of the high efficiency of the chaga extract obtained according to the invention, since antioxidants are used to treat and prevent diseases associated with the occurrence of free radical pathologies in the human body, such as cancer, radiation sickness, atherosclerosis, coronary heart disease and other

Using the invention allows:

- increase the antioxidant activity of the extract by 90%.

Using the method according to the invention for extracting chaga with an aqueous 7.5% solution of dimethyl sulfoxide, it can be used under industrial conditions to obtain chaga extracts with a high content of extractive substances and high antioxidant activity.

Claims (1)

A method of obtaining a chaga extract, including extracting chopped chaga, filtering out the entire extract to obtain a first extraction step and pulp, extracting the pulp to obtain an extract of a second extraction step and combining the extracts, characterized in that the chopped chaga is extracted with an aqueous 7.5% dimethyl sulfoxide solution.