RU2409591C2 - Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах - Google Patents

Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах Download PDF

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RU2409591C2
RU2409591C2 RU2006113695/10A RU2006113695A RU2409591C2 RU 2409591 C2 RU2409591 C2 RU 2409591C2 RU 2006113695/10 A RU2006113695/10 A RU 2006113695/10A RU 2006113695 A RU2006113695 A RU 2006113695A RU 2409591 C2 RU2409591 C2 RU 2409591C2
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antibody
buffer
hydroxyapatite
protein
resin
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RU2006113695/10A
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Russian (ru)
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RU2006113695A (ru
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Шуджун САН (US)
Шуджун САН
Кристофер ГАЛЛО (US)
Кристофер ГАЛЛО
Брайан КЕЛЛИ (US)
Брайан КЕЛЛИ
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Вайет
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
RU2006113695/10A 2003-10-27 2004-10-06 Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах RU2409591C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51401803P 2003-10-27 2003-10-27
US60/514,018 2003-10-27
US52333503P 2003-11-20 2003-11-20
US60/523,335 2003-11-20

Related Child Applications (1)

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RU2010139827/10A Division RU2573910C2 (ru) 2003-10-27 2010-09-24 Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах

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RU2006113695A RU2006113695A (ru) 2007-12-10
RU2409591C2 true RU2409591C2 (ru) 2011-01-20

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RU2006113695/10A RU2409591C2 (ru) 2003-10-27 2004-10-06 Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах
RU2010139827/10A RU2573910C2 (ru) 2003-10-27 2010-09-24 Удаление агрегатов с высокой молекулярной массой путем хроматографии на гидроксиапатитах

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US (2) US9469672B2 (ja)
EP (2) EP2336172B1 (ja)
JP (2) JP5004586B2 (ja)
KR (2) KR101221862B1 (ja)
CN (2) CN1898266B (ja)
AU (1) AU2004286938B2 (ja)
BR (2) BRPI0415887B1 (ja)
CA (1) CA2543193C (ja)
CO (1) CO5690649A2 (ja)
DK (2) DK2336172T3 (ja)
EC (1) ECSP066583A (ja)
ES (2) ES2530446T3 (ja)
IL (1) IL175229A (ja)
MX (1) MXPA06004472A (ja)
NZ (1) NZ547315A (ja)
PL (2) PL2336172T3 (ja)
PT (2) PT1678208E (ja)
RU (2) RU2409591C2 (ja)
WO (1) WO2005044856A2 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2714967C2 (ru) * 2013-12-27 2020-02-21 Чугаи Сейяку Кабусики Кайся Способ очистки антител с низкой изоэлектрической точкой
US10799589B2 (en) 2013-03-13 2020-10-13 Buzzard Pharmaceuticals AB Chimeric cytokine formulations for ocular delivery

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
CN1898266B (zh) * 2003-10-27 2011-09-21 惠氏公司 使用羟磷灰石层析除去高分子量聚集体
PT1869065T (pt) * 2005-03-11 2020-06-18 Wyeth Llc Método de cromatografia de partição fraca
EP1896506B1 (en) * 2005-06-17 2013-01-02 Janssen Alzheimer Immunotherapy Methods of purifying anti a beta antibodies
EP1893647A2 (en) * 2005-06-23 2008-03-05 MedImmune, Inc. Antibody formulations having optimized aggregation and fragmentation profiles
PE20070796A1 (es) * 2005-10-24 2007-08-15 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
EP1962907A2 (en) 2005-12-21 2008-09-03 Wyeth a Corporation of the State of Delaware Protein formulations with reduced viscosity and uses thereof
JPWO2007123242A1 (ja) 2006-04-25 2009-09-10 東ソー株式会社 ＩｇＧ精製用分離剤、及びそれを用いたＩｇＧ単量体の精製方法
EP2069387A4 (en) * 2006-06-14 2011-02-02 Glaxosmithkline Llc METHODS OF PURIFYING ANTIBODIES USING HYDROXYAPATITE CERAMIC
AU2007291283B2 (en) 2006-08-28 2012-09-20 Ares Trading S.A. Process for the purification of Fc-containing proteins
UA99263C2 (ru) * 2006-08-28 2012-08-10 Арес Трейдинг С.А. СПОСОБ ОЧИСТКИ Fc-СЛИТОГО БЕЛКА
TW200821026A (en) * 2006-09-08 2008-05-16 Wyeth Corp Arginine wash in protein purification using affinity chromatography
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
CN103555651A (zh) 2006-09-13 2014-02-05 Abbvie公司 细胞培养改良
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
CA2674213A1 (en) * 2007-01-26 2008-07-31 Merck Serono S.A. Purification of fc-taci fusion proteins using the oilbody technology
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
CA2703086C (en) * 2007-10-26 2014-11-25 Asahi Kasei Chemicals Corporation Method for purifying protein
JP5999899B2 (ja) 2008-04-08 2016-09-28 バイオ−ラッド ラボラトリーズ インコーポレーティッド 抗体のクロマトグラフィー精製法
KR20180033311A (ko) 2008-08-14 2018-04-02 제넨테크, 인크. 고유 단백질 대체 이온 교환 막 크로마토그래피를 이용한 오염물의 제거 방법
US9109010B2 (en) 2008-10-20 2015-08-18 Abbvie Inc. Viral inactivation during purification of antibodies cross reference to related applications
WO2010141039A1 (en) * 2008-10-20 2010-12-09 Abbott Laboratories Isolation and purification of antibodies using protein a affinity chromatography
WO2010056550A1 (en) 2008-10-29 2010-05-20 Wyeth Llc Methods for purification of single domain antigen binding molecules
RU2481824C2 (ru) 2008-10-29 2013-05-20 Аблинкс Н.В Препараты однодоменных антигенсвязывающих молекул
EP2350130B1 (en) * 2008-10-31 2018-10-03 Wyeth LLC Purification of acidic proteins using ceramic hydroxyapatite chromatography
CA2751000A1 (en) * 2009-03-11 2010-12-23 Wyeth Llc Methods of purifying small modular immunopharmaceutical proteins
CA2759502C (en) 2009-04-29 2021-02-09 Bio-Rad Laboratories, Inc. Purification of immunoconjugates
WO2011001963A1 (ja) 2009-07-03 2011-01-06 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
JP5914342B2 (ja) * 2009-10-20 2016-05-11 アッヴィ・インコーポレイテッド プロテインａアフィニティークロマトグラフィーを利用した抗ｉｌ−１３抗体の単離精製
KR101858266B1 (ko) 2009-12-18 2018-05-15 노파르티스 아게 친화성 크로마토그래피를 위한 세척용액 및 방법
EP2519537A4 (en) * 2009-12-29 2013-07-10 Reddys Lab Ltd Dr CLEANING PROTEINS
CA2787009C (en) * 2010-01-15 2018-04-03 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
KR20120132529A (ko) 2010-04-14 2012-12-05 에프. 호프만-라 로슈 아게 면역글로불린 응집물 제거
GB201006753D0 (en) 2010-04-22 2010-06-09 Biotest Ag Process for preparing an immunolobulin composition
WO2011156073A1 (en) * 2010-06-08 2011-12-15 Millipore Corporation Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts
US8895707B2 (en) * 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
JP2013540787A (ja) 2010-11-01 2013-11-07 ディーエスエム アイピー アセッツ ビー．ブイ． 単一ユニットイオン交換クロマトグラフィー抗体精製
WO2012106449A1 (en) 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
KR101901830B1 (ko) * 2011-06-08 2018-09-27 에이전시 포 사이언스, 테크놀로지 앤드 리서치 제한된 공수화 크로마토그래피를 통한 생물학적 산물의 정제
WO2012171853A1 (en) * 2011-06-16 2012-12-20 Dsm Ip Assets B.V. Single unit chromatography antibody purification
SG10201701224UA (en) 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
EP2855501B1 (en) 2012-05-30 2018-04-25 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
WO2014003142A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 抗体
JP2014105180A (ja) * 2012-11-27 2014-06-09 Hoya Corp モノマー化方法
EP2997041A4 (en) * 2013-05-13 2017-02-15 Medlmmune, LLC Separation of recombinant polyclonal antibody multimers with minimal separation of monomers
US10611826B2 (en) 2013-07-05 2020-04-07 Laboratoire Français Du Fractionnement Et Des Biotechnologies Affinity chromatography matrix
CA2910770C (en) 2013-07-12 2018-02-27 Merck Patent Gmbh Removal of fragments from a sample containing a target protein using activated carbon
JP6479305B2 (ja) * 2013-07-12 2019-03-06 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
SG10201804200WA (en) 2013-12-12 2018-07-30 Emd Millipore Corp Protein separations using an acrylamide containing filter
US9802822B2 (en) 2014-06-23 2017-10-31 Bio-Rad Laboratories, Inc. Apatite pretreatment
WO2015200278A1 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
CA2970732C (en) 2014-12-15 2023-05-16 Merck Patent Gmbh Target molecule capture from crude solutions
BR112017018365A2 (pt) * 2015-03-13 2018-04-10 Bristol-Myers Squibb Company uso de lavagens alcalinas durante cromatografia para remoção de impurezas
US11661438B2 (en) 2015-12-21 2023-05-30 Pfizer, Inc. Purification of antibody drug conjugates using a sodium phosphate gradient
US11919924B1 (en) * 2016-03-15 2024-03-05 Fresenius Kabi Deutschland Gmbh Methods of purifying and producing an adalimumab antibody
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
CN106977591B (zh) * 2017-05-04 2020-05-05 广州康盛生物科技股份有限公司 一种分离纯化重组葡萄球菌蛋白a的方法
WO2019040671A1 (en) * 2017-08-22 2019-02-28 Biogen Ma Inc. METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT
WO2019116096A1 (en) * 2017-12-15 2019-06-20 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Production of fc fragments
BR112020015603A2 (pt) * 2018-02-27 2021-01-05 Pfizer Inc. Purificação de anticorpo
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
JP2021519339A (ja) 2018-04-03 2021-08-10 メルク パテント ゲーエムベーハー Ｃｅｘクロマトグラフィー媒体及び生物製剤供給原料からの標的タンパク質の低塩溶出
JP6951051B2 (ja) * 2018-05-24 2021-10-20 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法
EP4061825A1 (en) 2019-11-22 2022-09-28 MorphoSys AG Method to increase antibody yield during ion exchange chromatography
JP2020099909A (ja) * 2020-03-17 2020-07-02 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 処理方法、生産方法およびハイドロキシアパタイト充填剤
TW202204423A (zh) 2020-03-30 2022-02-01 比利時商艾伯霖克斯公司 用於產生和純化多價免疫球蛋白單可變域的方法
WO2021220251A1 (en) * 2020-05-01 2021-11-04 Kashiv Biosciences, Llc An improved process of purification of protein

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US523335A (en) 1894-07-24 Air-bolt for flour-mills
US419964A (en) 1890-01-21 Transom-lifter
US428894A (en) 1890-05-27 Process of making tool-handles
US514018A (en) 1894-02-06 Edward j
US4745183A (en) * 1984-02-21 1988-05-17 Bio-Rad Laboratories, Inc. Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
JPH03291300A (ja) * 1989-12-28 1991-12-20 Mitsui Toatsu Chem Inc ポリクローナル抗体の分離方法
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
PE20020574A1 (es) * 2000-12-06 2002-07-02 Wyeth Corp Anticuerpos humanizados que reconocen el peptido amiloideo beta
DE60231651D1 (de) * 2001-12-21 2009-04-30 Immunex Corp Proteinreinigungsverfahren
DK1601697T3 (da) 2003-02-28 2007-10-01 Lonza Biologics Plc Oprensning af antistof ved protein A- og ionbytningskromatografi
CN1898266B (zh) * 2003-10-27 2011-09-21 惠氏公司 使用羟磷灰石层析除去高分子量聚集体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GIOVANNINI R. et al., "Comparison of different types of ceramic hydroxyapatite for the chromatographic separation ofplasmid DNA and a recombinant anti-rhesus D antibody", Bioseparation. 2000; 9(6): 359-68. GIOVANNINI R. et al., "Isolation of a recombinant antibody from cell culture supernatant: continuous annular versus batch and expanded-bed chromatography", Biotechnol Bioeng. 2001 Jun 20; 73(6): 522-9. *

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US10799589B2 (en) 2013-03-13 2020-10-13 Buzzard Pharmaceuticals AB Chimeric cytokine formulations for ocular delivery
RU2714967C2 (ru) * 2013-12-27 2020-02-21 Чугаи Сейяку Кабусики Кайся Способ очистки антител с низкой изоэлектрической точкой
US10654933B2 (en) 2013-12-27 2020-05-19 Chugai Seiyaku Kabushiki Kaisha Method for purifying antibody having low isoelectric point

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EP1678208A2 (en) 2006-07-12
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US20130197198A1 (en) 2013-08-01
BRPI0415887B1 (pt) 2021-01-26
KR101221862B1 (ko) 2013-01-14
CN102276717B (zh) 2015-09-09
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