RU2389081C1 - Apparatus for modelling of intero receptor thermal burn shock with surgery-substitute way in acute experiment in small laboratory animals - Google Patents

Abstract

FIELD: medicine. ^ SUBSTANCE: apparatus is represented as gastro-duodenal tube. Moistureproof heating spiral is designed on the working end of the tube, connected with heat controller and current power supply by virtue of cables inside of tube. The heating spiral is covered outside with flexible film fixed to the working end of the tube. ^ EFFECT: increase of experimentator's operational comfort, avoidance of direct contact of hot water with gastric mucosa, decrease of injury animals under investigation, increase of reproductibility, enlargement of amount of technologies for modelling of intero receptor thermal burn shock. ^ 1 dwg

Description

The invention relates to experimental medicine, namely to experimental resuscitation and pathological physiology. It can be used for modeling in small laboratory animals interoreceptor thermal burn shock in order to study the stages of its development, pathogenesis, treatment options and further transfer of their results to the human body.

A device for modeling interoreceptor thermal burn shock (Turkmenov MT "Principles of physiological modeling and the results of the study of some threatening conditions in the experiment", Frunze, "Ilim", 1975, pp. 107-108), taken as a prototype. It consists of a gastric tube and a graduated funnel.

The simulation method using the known device is as follows. In a laboratory animal, the esophagus is exposed, bluntly cleaned of surrounding fiber and taken on gauze loops. Then the esophagus is incised and a thick gastric tube is inserted into the gap formed. Then, through a probe using a graduated funnel, hot water (90-95 degrees) is rapidly poured into the stomach (within 1.5-2 minutes) at the rate of 100 ml per 1 kg of animal weight. After 1-5 minutes (the exposure time is selected depending on the severity of the shock), the injected water is removed from the stomach by the principle of a siphon (lowering the funnel). To obtain fleeting reactions, water is not evacuated.

The above method of modeling interoreceptor thermal burn shock using a known device, with all its positive aspects, has significant disadvantages. The method is developed for large laboratory animals, for example dogs, and cannot be used in rats. It reproduces the model in a surgical, “bloody” way, it is technically difficult to do, and at the same time it significantly “lubricates” the clinical picture of the interoreceptor thermal burn shock in the experimental animal due to the fact that hot water, having direct contact with the gastric mucosa, has not only thermal, but and hydrolytic effect. Hot water introduced through a probe has a thermal effect on the esophagus, increasing the trauma of the animal. In addition, it seems difficult to accurately expose the damaging effects of hot water on the stomach, since it takes a certain time for its introduction and excretion. Therefore, the method of modeling interoreceptor thermal burn shock using a known device cannot improve the reproducibility of the method.

The objective of the invention is to create a simple device for modeling interoreceptor thermal burn shock without opening the cavities of the experimental animal and suturing, without direct contact of hot water with the gastric mucosa, without thermal effects on the esophagus and with high reproducibility in various types of laboratory animals.

The technical result is an increase in the convenience of work, the exclusion of direct contact of hot water with the gastric mucosa, a decrease in the morbidity of the animals being studied, an increase in reproducibility, and an expansion of the arsenal of technical means for modeling interoreceptor thermal burn shock.

The drawing shows a diagram of the proposed device.

The device is implemented as a gastric tube 1 of the corresponding diameter. At the working end 2 of the probe, a moisture-proof heating element 3 is made, connected via wires 4 located inside the probe 1, to the temperature controller 5 and to the current source 6. The heating element 3 is coated on the outside with an elastic film 7 that is tightly fixed to the working end 2 of the probe. The elastic film 7 can to be performed, for example, from part of a condom.

The present invention is used as follows.

A small laboratory animal, for example a rat, under anesthesia is fixed on the operating table. Probe 1 is introduced through the pharynx to the stomach. Then, a sufficient amount of water is filled into the film of the rat 7 with a syringe through probe 1 into the film 7 (approximately 0.5 ml per 10 g of animal weight). Turn on the heating element 3 and set the thermostat 5 in the heating mode of the introduced water to the temperature necessary for the duration of the burn shock exposure, in the range of 90-95 ° C. Depending on the severity of the simulated shock, the exposure time of hot water (exposure) is from 0.5 to 5 minutes. Then, the current source 6 is turned off, the water introduced into the stomach is removed using the syringe used, and probe 1 is carefully removed.

Next, they observe the increase in clinical symptoms of thermal burn shock in a laboratory animal and are regularly examined, including using electrophysiological methods.

The proposed device allows non-surgical modeling of interoreceptor thermal burn shock of varying severity depending on the time of the thermal effect of hot water on the stomach in small laboratory animals, it is uncomplicated and consists of a minimum of constituent elements.

Claims (1)

A device for simulating an interoreceptor thermal burn shock, consisting of a gastric probe, characterized in that a moisture-proof heating element is made on the working end of the probe, coated on the outside with an elastic film that is tightly fixed to the working end of the probe and connected to the temperature regulator and the source by means of wires placed inside the probe current.

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