RU2360669C1 - Atherosclerosis nutritive prevention and treatment method - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to cardiology, and concerns atherosclerosis nutritive prevention and treatment ensured by introduction of BAAs "Pharmanex": LifePak dosed 1 pill in the morning and in the evening, MarineOmega® dosed 1 capsule in the morning; Tegreen⁹⁷ dosed 1 capsule in the morning every second day within 8 weeks.

EFFECT: scheduled introduction of said specific BAAs provides mutual potentiation of their anti-oxidant, hypolipidemic and hypotensive properties.

3 tbl, 2 ex

Description

The invention relates to medicine and can be used in cardiology, endocrinology, neurology, valeology, preventive and rehabilitation medicine for the prevention and treatment of atherosclerosis in people of working age.

Known methods for the prevention and treatment of atherosclerosis by oral administration of carotenoid preparations in a daily dose of 5-10 mg / kg body weight, the adequacy of the use of which is estimated by the serum lycopene content (RF patent 2095059, A61K 31/07). The known method by the combined introduction of beta-carotene 0.25-500 mg and vitamin E 5-5000 mg or beta-carotene 0.25-500 mg and aspirin 5-2400 mg (RF No. 2126679).

A known method for the prevention and treatment of atherosclerosis by taking omega-3 polyunsaturated fatty acids (PUFA) “Eikonol” with omega-3 PUFA content of at least 18% EPA and 10% DHA in the lipid portion of 30 ml per day, regardless of food intake, taken prototype (RF Patent No. 2055590).

A known method for the prevention and treatment of atherosclerosis, which consists in taking compositions containing cocoa polyphenols in the form of procyanidin monomers and oligomers, including catechin and epicatechin monomers and oligomers (RF Patent No. 2303373).

There is a method consisting in the implementation of a diet based on a lacto-vegetarian diet with a high content of vegetable proteins and fats (polyunsaturated fatty acids), rich in multivitamins and minerals (RF Patent No. 2060012).

There is a method consisting in the use of a mixed anti-atherosclerotic diet No. 10, developed by the Institute of Nutrition (see Handbook of Dietetics. M: Medicine, 1992, p. 144).

A known method for the prevention and treatment of atherosclerosis through the use of the "Mediterranean diet", rich in antioxidants of fruits and vegetables, polyunsaturated fatty acids from olive oil and polyphenols of red wine.

Closest to the proposed method is the prevention and treatment of atherosclerosis by taking the biologically active food supplement Life Pack® (NSE products, Inc, USA) to increase the antioxidant ability of blood serum and reduce the oxidation of low density lipoproteins in healthy non-smokers (Smidt CR , Seidehamel RJ, Devaraj S., Jialal I. The effects of a nutritionally complete dietary supplement (LifePak®) on antioxidant status and LDL-oxidation in healthy nonsmokers // FASEB J. 1999. - V. 13 (4). - A . 546).

A new technical problem is to increase the efficiency of the method by creating conditions for enhancing the antioxidant effect on the state of the cardiovascular system

To solve the problem in the method of nutritional prevention and treatment of atherosclerosis by administering vitamin-antioxidant dietary supplements, Farmaneks dietary supplements are introduced: Life Pack 1 tavliot in the morning and evening, Marina Omega® 1 capsule in the morning; "T-green ⁹⁷ ", 1 capsule in the morning every other day for 8 weeks.

The method is as follows. Patients belonging to a risk group or already diagnosed with atherosclerosis are given nutritional prophylaxis and correction of their antioxidant status and risk factors for atherosclerosis, which consists in the introduction of vitamin-antioxidant dietary supplements with the reproduction of the main components of the Mediterranean diet, namely: Pharmanex dietary supplements are administered (NSE products Inc., USA): Life Pack 1 tavliota in the morning and evening. “Marina Omega®”, 1 capsule in the morning; "T-green ⁹⁷ ", 1 capsule in the morning every other day for 8 weeks.

The method consists in the complex use of biologically active food additives containing carotenoids, omega-3 polyunsaturated fatty acids and polyphenols, reproducing components equivalent to high consumption of fruits and vegetables, high consumption of olive oil, walnuts and regular consumption of red wine) and clinical effects (lipid-correcting , antioxidant, antihypertensive) Mediterranean diet.

Use dietary supplements "NSE products, Inc" included in the radar of the Russian Federation:

Lifepack-1 tavliota contains% of the daily rate:

Lycopene 12.5 Alpha lipoic acid 12.5 Beta carotene 10 Lutein 5

2 tablets were used per day, so the percentage doubles and, due to the integrated use of additional antioxidant dietary supplements, the clinical effect is enhanced.

Lifepack tavliots contained% of the daily rate:

Lycopene 25 Alpha lipoic acid 25 Beta carotene twenty Lutein 10

Marina Omega is a mixture of fish oil (1100 mg), krill oil (50 mg) and vitamin E (5 ME) per capsule.

The content of PUFA omega-3, not less than 30%.

Eicosapentaenoic acid, not less than 18%.

Docosahexaenoic acid, not less than 12%.

Supplements are used in 1 capsule per day, however, the effectiveness of application, due to the content of the animal antioxidant astaxanthin in krill oil, as well as due to the integrated use of additional antioxidant dietary supplements, is enhanced.

Tigrin containing 250 mg of green tea leaf extract and at least 160 mg of catechins in one capsule.

The dose is 1 capsule every other day, however, the effectiveness of the application, due to the high content of catechins (polyphenols) in each capsule, and also due to the integrated use of additional antioxidant dietary supplements, is significantly enhanced, as the study showed.

It is known that hyperactivation of lipid peroxidation, excessive accumulation of free radicals, impaired permeability of the vascular wall, as well as endothelial dysfunction [1, 2] directly lead to a decrease in energy production in mitochondria, psychophysical maladaptation and early aging of the body [3]. At the same time, high oxidative stress and low antioxidant protection cause premature development of coronary heart disease and arterial hypertension (AH) [1]. In this regard, an early assessment of antioxidant protection and its correction is especially relevant. Recent publications have found that lipid peroxidation metabolites (LPO) have a clear relationship with the level of antioxidants of low density lipoproteins (LDL), an independent association with total cholesterol (OXS), triglycerides (TG), high density lipoproteins (HDL), smoking and systolic blood pressure (SBP) [4]. Moreover, in patients with cardiovascular diseases (CVD), a direct correlation was found between the levels of reactive oxygen metabolites and diene conjugates in LDL [51. In addition, it has been proved: among the causes of low antioxidant protection, the most significant and easily corrected is a deficiency of antioxidants in food. So, at the European Congress of Cardiology in 2004, low consumption of fruits and vegetables was named one of the nine risk factors for myocardial infarction

[6]. In addition to their own antioxidant systems, exogenous antioxidants, vitamins A, C, and E, should be ingested, deficiency of which is always detected in patients with coronary artery disease [7]; therefore, high hopes were placed on their cytoprotective effectiveness, but the results of a randomized multicenter study Heart Protection Study did not confirm such properties [cit. 8]. As a result, it was concluded that evidence-based medicine requires the transfer of CVD patients from fruitless attempts to treat vitamins with statin therapy [9]. Nevertheless, today it has been established that phytonutrients from vegetables / fruits have more pronounced antioxidant effects compared to vitamins: numerous carotenoids, flavonoids, alpha-lipoic acid, etc. [10, 11]. It is their increased content in a Mediterranean diet rich in omega-3 fatty acids from seafood and olive oil, as well as red wine polyphenols, that helps prevent CVD [12], which was confirmed in the PREDMED study (Prevencion con Dieta Mediterranea) with 772 Spaniards 55 -80 years with diabetes and other CVD risk factors. Patients were randomly assigned either low-fat food (control) or the Mediterranean diet: 1 liter of olive oil per week or 30 g of walnuts, after 3 months such a diet led to a significant decrease in blood glucose, systolic blood pressure and total cholesterol.

The essence of the proposed method is that it is proposed, using the original regimen of vitamin-antioxidant dietary supplements, to reproduce the main components of the Mediterranean diet to obtain corrective effects on antioxidant defense, oxidative stress, lipid spectrum, as well as levels of other risk factors for the development of atherosclerosis (FRA) in people of working age age from the perspective of evidence-based medicine.

The proposed method execution mode is based on the analysis of clinical observation results, for which a randomized cohort blind trial study was performed in a workforce of 450 employees with a relatively uniform educational level. Response to voluntary participation in the study was 32.2% (145 people). Randomization was carried out by random numbers: out of 145, 45 employees were included. The representativeness of the sample was 10% (45 people) of the total number of employees. To obtain informed consent, discussions were held on the purpose of the test. Screening and blood sampling from a vein was performed before and after an 8-week use of antioxidant dietary supplements. At this time, the subjects led a normal lifestyle, not adhering to any dietary restrictions. To increase the reliability of the results of the work, “triple blind” control was carried out: 1) one group of scientists screened and processed the clinical material; 2) processing the results of biochemical testing - another group; 3) statistical analysis was performed by a third group of specialists.

Thus, 45 men and women from 25 to 62 years old (mean age 44.6 ± 9.1 years) with FRA and CVD were examined. The main group included 30 people, of which subgroup A included 16 healthy participants with FRA (9 men and 7 women, average age 43.9 ± 10.7 years): a low level of consumption of fruits and vegetables was found in 16 (100%) ; high normal blood pressure (VNAD) in 16 (100%); dyslipidemia (DLP) in 12 (75%); smoking in 10 (62.5%), overweight (BMI) in 9 (56.2%) people. Subgroup B included 14 patients (8 men and 6 women, average age 47.3 ± 10.0): Stage II hypertension in combination with grade II obesity was found in 11 (78.5%) patients; IHD: angina pectoris II FC, post-infarction cardiosclerosis in patients with stage II hypertension, were detected in 3 patients. Among the main FRAs in subgroup B were noted: DLP in 14 (100%); smoking in 7 (50.0%) patients; low consumption of fruits and vegetables in 14 patients (100%). The control group included 15 healthy individuals with FRA (9 men and 6 women, average age 45.8 ± 8.5). At the same time, a low level of fruit and vegetable consumption was detected in 15 (100%), VNAD in 12 (80%), DLP in 12 (80%), smoking in 11 (73.3%), and BMI in 8 (53.3 %) volunteers (66.6%). Formed subgroups and control group were comparable by gender, age, and FRA.

Quantitative determination of the total antioxidant ability (OAS) and the level of peroxide (USP) of EDTA blood plasma was carried out using Labor Diagnostika Nord Gmbh & Co. test systems. KG, Nordhom Germany and Biomedica OxyStat Biomedica-Gruppe, Austria. This fragment was performed at the Department of Functional Laboratory Diagnostics, State Research Institute of Cardiology, TNTs SB RAMS. Samples of EDTA blood plasma taken from subjects before and after using dietary supplements, to increase the accuracy of measurements, were frozen at t = -20 ° C. Further, following the prescribed instructions, including measurement in duplicates, EDTA plasma samples were examined simultaneously. The level of OSA at values <1.0 mmol / L was considered low, at 1-1.3 mmol / L was considered moderately low, at values> 1.3 mmol / L - sufficient. Values of <400.0 μmol / L were considered normal levels of USP in healthy individuals. Fruit and vegetable consumption was low if less than 5 apples or 5 servings of vegetables were eaten daily [14].

To reproduce the main components and effects of the Mediterranean diet, a special selection of Farmanex (NSE products Inc., USA) dietary supplements included in the Russian radar was used [15]. Life Pak® [14] - a source of beta-carotene carotenoids, lycopene lutein, as well as alpha lipoic acid, was used for 1 tavliot in the morning and evening. “Marina Omega®” - krill oil, as a source of omega-3 acids, astaxanthin carotenoid and vitamin E, was used 1 capsule in the morning; Tigrin ⁹⁷ ®, a dry extract of green tea, as a source of catechins and polyphenols, was used 1 capsule in the morning every other day. Thus, the faces of the main group added “triple set” daily to their food for 8 weeks: Life Pack®, Tigrin ⁹⁷ ® and Marina Omega®. The dietary supplement control group did not accept, because it was formed to evaluate the effectiveness of the corrective effect, reproduced using dietary supplements, of the components of the Mediterranean diet.

Statistical analysis of indicators, observation groups included: average value, standard deviation, arithmetic error / coefficient of variation. Comparison of the mean values in the groups was performed by the nonparametric van der Waerden criterion [16], because signs did not have a normal distribution. The intensity of the correlation was estimated using the Spearman rank correlation coefficient. The critical level of statistical significance was taken p = 0.1. The parameter values obtained in the work are presented as M ± m. Statistical analysis was performed by the BIOSTATISTICS Center (E-mail: point@stn.tomsk.ru) using SAS 9 and SPSS 15 packages.

Research results and discussion

Anthropometry data, lipid spectrum, antioxidant / oxidant status in individuals of the main (subgroup A and B) and control groups before the use of dietary supplements are shown in table 1. It was found that healthy individuals with FRA in both subgroup A and control showed the same type of shifts typical for DLP: increased levels of cholesterol, LDL, but with normal levels of HDL, atherogenic index (IA). BP indicators corresponded to the level of VNAD. Against the background of normal peroxide content, in subgroup A and control group the level of OSA was low. A similar picture was revealed in subgroup B: the levels of OSA, SAD, LDL, HDL and IA, with the exception of TG, were deviated from the norm.

Anthropometry data, lipid spectrum, antioxidant / oxidant status in individuals with subgroup A AFA after administration of dietary supplements are shown in Table 2. It was found that the level of OSA increased by 36.4% (p = 0.005), and USP decreased by 35.3% ( p = 0.01). At that, by 12.7% (p = 0.01), 12.2% (p = 0.04), the levels of SBP and GCS decreased, respectively. Similar shifts after the use of dietary supplements were identified in subgroup B (Table 3). The OSA indicator increased by 30.3% (p = 0.008), and IA decreased by 30.1% (p = 0.002). By 32.4% (p = 0.009), 13.7% (p = 0.02) and 11.0% (p = 0.04), respectively, the levels of TG, OXS and SBP decreased. HDL content increased by 32.8% (p = 0.02) against the background of a not significant increase in USP.

It is known that reactive oxygen species (ROS) arise in the cell as a result of chemical reactions of O ₂ with respiratory chain enzymes or enzyme systems. There are three categories of ROS [17]: primary, secondary and tertiary. Primary ROS include superoxide anion, which is then converted to hydrogen peroxide, hypochlorous acid and hypochlorite. With weak neutralization of superoxide, its excess, interacting with NO, forms peroxynitrite or converts Fe ³⁺ to F ²⁺ , which when combined with Н ₂ O ₂ and HCIO forms a hydroxyl radical (OH *) and / or, when combined with lipoperoxides, a dipoxyl radical (LO *), it is these ROS that are secondary radicals that can damage lipid membranes, DNA molecules, proteins and carbohydrates. The antioxidant system of the body consists of enzymes and low molecular weight substances. Superoxide dismutase is the first defense link that converts superoxide to H ₂ O ₂ . Hydrogen peroxide is electrically neutral and can either leave the cell or, under the influence of the catalase enzyme, turn into H ₂ O and O _2. In addition to enzyme systems, the antioxidant ubiquinone is synthesized in the membranes. At the same time, various carotenoids and flavonoids found in vegetables, fruits and red wine have a powerful antioxidant property. The given excursion allows us to understand that the use of test systems for assessing OSA and USP, as accurately as possible, made it possible to assess the overall antioxidant / oxidative status in both individuals with FRA and CVD patients before and after the use of dietary supplements. Confirmation of this turned out to be rather close inverse correlations between the level of OSA and USP in individuals with FRA (r = -0.58; p = 0.02) and CVD patients (r = -0.62; p = 0.001). Moreover, the correlations turned out to be stronger in women, especially after using dietary supplements: (in women with FRA before correction r = -0.76; p = 0.04, after correction r = -0.94; p = 0.001; in women with CVD before correction r = -0.95; p = 0.04; after correction r = -0.97; p = 0.001). It is significant that in men these connections were weaker and were noted before the use of dietary supplements: in individuals with FRA (r = -0.66; p = 0.05), in patients (r = -0.76; p = 0, 02). The differences found, in our opinion, are explained by the fact that in subgroup B there were men with a pronounced cardiac pathology, who after the use of dietary supplements had an increase in the level of peroxide in the blood. This fact is regarded by us, of course, as positive. Since CVD levels were low in CVD individuals, it can be assumed that they had a deficiency of enzymatic and non-enzymatic antioxidant systems with the creation of conditions for excessive ROS formation. In this regard, we can confidently assume that the positive dynamics from the level of OSA was due, primarily, due to the highly effective antioxidant additives Farmaneks. Naturally, in this situation, the neutralization of the excess of ROS could lead to a slight increase in the level of peroxide. It is likely that against the background of the restored antioxidant activity of blood in CVD individuals, it takes longer than 8 weeks for the excess H ₂ O _{2 to} neutralize to H ₂ O and O ₂ . As for the other reason for the differences found in the relationship between OSA and USP, it can probably be explained by the fact that women follow diet recommendations more, have a wider list of healthy behavior skills and responsibility in maintaining and promoting health. In addition, other correlation relationships were obtained in the work, which only confirm the data of other studies of the relatively high efficiency of both dietary supplements and the Mediterranean diet in particular. For example, in subgroup A, before the use of dietary supplements, a direct correlation was found between OSA and HDL (r = 0.67; p = 0.005). And in individuals of subgroup B, an inverse relationship between USP and HDL (r = -0.71; p = 0.02) and a direct relationship between USP and IA (r = 0.74; p = 0.01) were found. The analysis of correlations depending on gender turned out to be just as significant. So, in CVD men, before using dietary supplements, there was a direct correlation of USP with IA (r = 0.85; p = 0.03) and feedback of USP with HDL (r = -0.89; p = 0.01). Whereas, after the use of dietary supplements, a direct relationship was found between USP and LDL (r = 0.79; p = 0.01), as well as an inverse relationship between OSA and CAD (r = -0.91; p = 0.01). As for women, in subgroup A, even before the use of dietary supplements, direct associations of USP with IA (r = 0.76; p = 0.04), USP with LDL (r = -0.79; p = 0.006) and feedback between USP and HDL (r = -0.81; p = 0.01). It is characteristic that in sick women with CVD, an inverse relationship was also observed between USP and HDL (r = -0.91; p = 0.01) and also before the use of antioxidant dietary supplements.

In connection with the foregoing, it should be noted that in the process of clinical testing it was possible to obtain almost all antiatherosclerotic effects of the Mediterranean diet reproduced with the help of dietary supplements. Moreover, it was found that the antioxidant activity of Life Pak® extends not only to healthy non-smokers [14], not only to healthy people with combined FRA, but also to sick people with CVD. In other words, it has been proven that the carotenoids that make up Life Pack® are the most important class of nutrient antioxidants that positively affect the function of the heart and blood vessels. Moreover, the antioxidant activity of Life Pak®, enhanced by the use of Marine Omega®, not only decreased the oxidation of lipids [14], but also caused lipid-correcting effects, directly affecting the intensity of lipid peroxidation [7]. Another key point of using “Marine Omega®” was the additional antihypertensive effect. For example, it was shown that a decrease in blood pressure to the optimal level was possible not only in healthy individuals with VNAD, but also in patients with hypertension. It should be noted that patients with hypertension continued (not regularly!) To use antihypertensive drugs before and during the test. But, since their level of SBP before and after the use of dietary supplements differed, it would be reasonable to consider that the decrease in SBP occurred due to the hypotensive effect of the latter. This is completely consistent with the opinion of other authors who believe that the modifying effect of lipid peroxidation metabolites on constriction of arterioles and an increase in total peripheral resistance is the main link in the participation of lipid peroxidation in the progression of hypertension [18]. Therefore, the use of Tigrin ⁹⁷ ® with a high level of polyphenols, of which 65% are catechins, only supports this point of view. Studies of the University of Kansas (in vitro, 1997) [19] found that the antioxidant ability of Tigrin ⁹⁷ ®, due to the content of the catechin antioxidant epigallocatechin-3-gallate, in equivalent doses, is several times stronger than the activity of vitamin C, E, as well as wine polyphenols, and when you consider that Tigrin ⁹⁷ ® does not contain caffeine, the advantages and prospects of its use are more than obvious. This circumstance made it possible not only to prescribe Tigrin ⁹⁷ ® to individuals with AH, but also to expect an increase in antioxidant, lipid-correcting and hypotensive effects due to the proven effect of polyphenols on the activity of NO synthase [20].

Example 1. Subject K., 29 years old, office employee. Height: 172 cm, weight: 89 kg, Quetelet index - 30.0 kg / m ² . No complaints. A low level of consumption of fruits and vegetables was revealed (1-3 servings of salad, 1 apple or banana 2-3 times a week). The heredity of atherosclerosis is burdened, smokes more than 15 cigarettes per day. Not physically active. Single. At the time of the examination, blood pressure 138/85 mm Hg, heart rate 68 beats / min. Lipid spectrum: total cholesterol - 5.9 mmol / L, low density lipoproteins (LDL) - 3.9 mmol / L, triglycerides (TG) - 1.7 mmol / L, high density lipoproteins (HDL) - 1.0 mmol / l The atherogenic index is 4.9. The total antioxidant ability of blood plasma is 1.02 mmol / L (normal> 1.3). The level of peroxides in the blood plasma is 470 μmol / L (normal <400 μmol / L). An objective examination of the pathology of the internal organs was not detected.

Given the decrease in total antioxidant ability and an increase in the level of peroxides in the blood, the presence of dyslipidemia and high normal blood pressure, and the presence of other combined risk factors for the development of atherosclerosis, including hereditary burden of atherosclerosis, smoking and low levels of fruit and vegetables, it was recommended that the test subject 2-month use of bioactive additives Pharmanex "Life Pak®" one tavliote morning and evening, "Marina Omega®" one capsule in the morning and "T-green ^{9 /} ®» one capsule in the morning after Shade.

2 months after the regular intake of Farmanex dietary supplements, a repeated examination of the subject revealed that the blood pressure level dropped to 125/70 mm Hg. Total cholesterol decreased to 4.9 mmol / L, LDL and TG decreased to 2.9 and 1.2 mmol / L, respectively, HDL increased to 1.3 mmol / L, and the atherogenic index dropped to 2.7. The total antioxidant ability of blood plasma increased to 1.47 mmol / L. The content of peroxides in blood plasma decreased to 320 μmol / L.

This clinical example shows that the proposed regimen of taking the “triple set” of Farmanex antioxidant dietary supplements can reduce the intensity of the existing risk factors for developing atherosclerosis, including increasing the antioxidant ability and blood plasma peroxides, and it has a pronounced lipid-correcting and noticeable hypotensive effect, which directly indicates the possibility of active preventive measures in relation to the prevention of both atherosclerosis and cardiovascular disease generally in healthy people of working age with combined cardiovascular risk factors.

Example 2. Subject E., 54 years old, office employee. Suffers from coronary heart disease: angina of exertion III FC for 6 years, Hypertension 9 years. Two years ago, he suffered an acute large-focal posterior myocardial infarction. PA the moment of the examination, blood pressure level 170/100 mm Hg Height 165 cm, weight 86 kg. Ketle index 31.5 kg / m ² , heart rate 87 beats / min. Uses nitroglycerin up to 5 times a week for 1-3 tablets, however, it uses hypotensive and antianginal drugs on a case by case basis. Smokes up to 20 cigarettes per day and complains of shortness of breath during physical exertion, accompanied by pain in the heart. The consumption of fruits and vegetables is low (3 times a week vegetable salad). Not physically active. Married. Total cholesterol - 6.7 mmol / L, LDL - 4.3 mmol / L, TG - 2.1 mmol / L, HDL - 0.8 mmol / L. The atherogenic index is 7.3. The indicator of the total antioxidant ability of blood plasma is 0.9 mmol / L. The content of peroxides in the blood is 430 μmol / L. An objective examination of the internal organs revealed signs of chronic hepatitis and cholecystitis without exacerbation.

Given the presence of pronounced cardiovascular pathology, a decrease in antioxidant protection, significant dyslipidemia, high blood pressure, and a high risk of repeated cardiac events, including fatal ones, a 2-month course of Pharmanex bioactive supplements is recommended for the patient to improve nutritional and antioxidant status. Life Pak® was used one tavliot in the morning and in the evening, Marina Omega® one capsule in the morning and Tigrin ⁹⁷ ® one capsule in the morning every other day.

2 months after the regular administration of Farmanex antioxidants, a positive dynamics was observed during the repeated examination of the patient. In particular, blood pressure decreased to 145/90 mm Hg. Total cholesterol decreased to 5.1 mmol / L, LDL and TG decreased to 3.5 and 1.5 mmol / L, respectively, HDL content increased to 1.2 mmol / L, while the atherogenic index decreased to 3, 2. The total antioxidant ability of blood plasma increased to 1.42 mmol / L. The level of peroxides in the blood remained almost the same. However, at the same time, nitroglycerin intake decreased to 2-3 times a week, shortness of breath significantly decreased, overall well-being and tolerance of psycho-emotional and physical exertion significantly improved.

This example indicates the high therapeutic efficacy of the proposed method, even in patients with combined cardiac pathology. II, since such a noticeable positive trend was achieved already 2 months after the administration of Farmanex supplements, it can be assumed that the proposed “triple set” of Farmanex reproduces not only the components of the Mediterranean diet, but also its main anti-atherosclerotic therapeutic effects.

Thus, a method is proposed that is based on a new approach to the effective use of dietary supplements not only for prophylactic but also for therapeutic purposes. The data obtained allow us to conclude that the proposed method allows to obtain the effects obtained in the application of the Mediterranean diet.

The advantages of the proposed method:

- you do not need to follow a strict diet, it is enough to adhere to the principles of healthy nutrition within the narrow-minded approach;

- the intake of dietary supplements in the morning and evening, firstly, is sufficient to obtain positive clinical effects and, secondly, is not burdensome;

- dosages of the proposed dietary supplements are strictly standardized;

- regular addition of the proposed dietary supplements to the daily diet effectively improves nutritional status and easily reproduces the main components of the Mediterranean diet;

- the use of the proposed dietary supplements for two months does not cause, as the study showed, no side effects.

Based on the analysis of the results, it can be predicted that a regular (2-3 times a year) 2-month corrective diet course of the proposed dietary supplements will increase the effectiveness of both preventive and therapeutic anti-atherosclerotic measures.

The proposed method for the prevention and treatment of atherosclerosis, by special selection of vitamin-antioxidant dietary supplements with reproduction of the main components of the Mediterranean diet, corrects antioxidant protection, oxidative stress, lipid spectrum, as well as levels of other risk factors for the development of atherosclerosis (FRA) in people of working age. The combined use of Life Pak®, Marine Omega® and Tigrin ⁹⁷ ® effectively increases the antioxidant status in healthy individuals with PDA and in patients with CVD, causes a lipid-correcting and hypotensive effect and is an effective way of active prevention and treatment atherosclerosis.

Table 1. Anthropometric data, lipid spectrum, antioxidant / oxidative status in individuals of the main (subgroup A and B) and control groups before corrective measures (M ± m) Indicator Subgroup A (n = 16) Subgroup B (n = 14) Control Group (n = 15) Significance level between A and B Age years 43.9 ± 10.7 47.3 ± 10.0 45.8 ± 8.5 0.19 Height, cm 171.2 ± 8.6 168.3 ± 10.3 170.7 ± 8.3 0.41 Weight, kg 75.4 ± 11.6 83.0 ± 10.8 82.9 ± 15.8 0.18 Ketle index, kg / m ² 25.5 ± 3.1 29.5 ± 1.3 28.5 ± 6.0 0.04 GARDEN, mmHg 138.9 ± 5.0 146.1 ± 12.2 134.0 ± 4.1 0.12 DBP, mmHg 81.8 ± 2.7 84.8 ± 12.4 85.0 ± 8.5 0.33 Heart rate, beats / min 84.0 ± 2.9 80.0 ± 9.0 78.8 ± 9.3 0.28 OHS, mmol / l 5.7 ± 0.3 5.8 ± 0.3 5.7 ± 0.4 0.69 TG, mmol / l 135 ± 0.14 1.45 ± 0.21 1.54 ± 0.22 0.28 LDL, mmol / l 3.73 ± 0.3 3.82 ± 0.24 3.76 ± 0.28 0.26 HDL, mmol / l 1.28 ± 0.1 0.97 ± 0.09 * 1.31 ± 0.1 0.005 Index 3.42 ± 0.4 4.71 ± 0.6 * 3.48 ± 0.36 0.002 atherogenicity 1.02 ± 0.07 1.09 ± 0.07 1.12 ± 10.0 0.36 OSA, mmod / l 364.3 ± 32.8 333.6 ± 38.5 328.5 ± 37.2 0.52 USP, micromol / l Note: (*) - significance level <0.05 between subgroup B and control group. There were no significant differences between subgroup A and control group.
Here and in table 2, 3, the OSA indicator - reflects the total antioxidant ability of blood plasma, and OPS - the level of peroxide in blood plasma.

Table 2. Anthropometric data, lipid spectrum, antioxidant / oxidative status in healthy individuals with FRA (subgroup A) after corrective measures (M ± m) Indicator Before correction (n = 16) After correction (n = 16) Control Group (n = 15) Significance level (before and after) Age years 43.9 ± 10.7 43.9 ± 10.7 45.8 ± 8.5 - Height, cm 171.2 ± 8.6 171.2 ± 8.6 170.7 ± 8.3 - Weight, kg 75.4 ± 11.6 74.5 ± 10.8 82.9 ± 15.8 0.72 Ketle index, kg / m ² 25.5 ± 3.1 25.2 ± 2.9 28.5 ± 6.0 0.77 GARDEN, mmHg 138.9 ± 5.0 121.3 ± 2.7 * 134.0 ± 4.1 0.01 DBP, mmHg 81.8 ± 2.7 78.2 ± 1.6 85.0 ± 8.5 0.11 Heart rate, beats / min 84.0 ± 2.9 77.2 ± 4.6 78.8 ± 9.3 0.11 OHS, mmol / l 5.7 ± 0.3 5.0 ± 0.3 * 5.7 ± 0.4 0.04 TG, mmol / l 1.35 ± 0.14 1.19 ± 0.12 * 1.54 ± 0.22 0.12 LDL, mmol / l 3.73 ± 0.3 3.32 ± 0.23 3.76 ± 0.28 0.22 HDL, mmol / l 1.28 ± 0.1 1.34 ± 0.1 131 ± 0.1 0.31 Index 3.54 ± 0.4 3.17 ± 0.38 3.48 ± 0.36 0.54 atherogenicity 1.02 ± 0.07 1.39 ± 0.07 * 1.12 ± 10.0 0.005 OSA, mmol / l 364.3 ± 32.8 235.8 ± 22.3 * 328.5 ± 37.2 0.01 USP, micromol / l Note: (*) - significance level <0.05 between subgroup A after corrective measures and control group.

Table 3. Anthropometric data, lipid spectrum and antioxidant / oxidant status in patients (subgroup B) after corrective measures (M ± m) Indicator Before correction (n = 14) After correction (n = 14) Control Group (n = 15) Significance level (before and after) Age years 47.3 ± 10.0 47.3 ± 10.0 45.8 ± 8.5 - Height, cm 168.3 ± 10.3 168.3 ± 10.3 170.7 ± 8.3 - Weight, kg 83.0 ± 10.8 81.7 ± 11.5 82.9 ± 15.8 0.58 Ketle index, kg / m ² 29.5 ± 1.3 28.7 ± 1.4 28.5 ± 6.0 0.67 GARDEN, mmHg 146.1 ± 12.2 130.0 ± 10.5 134.0 ± 4.1 0.04 DBP, mmHg 84.8 ± 12.4 85.6 ± 2.9 85.0 ± 8.5 0.44 Heart rate, beats / min 80.0 ± 9.0 76.1 ± 9.0 78.8 ± 9.3 0.19 OXS, mmod / l 5.8 ± 0.3 5.0 ± 0.9 * 5.7 ± 0.4 0.02 TG, mmol / l 1.45 ± 0.21 0.98 ± 0.14 * 1.54 ± 0.22 0.009 LDL, mmol / l 3.82 ± 0.24 3.49 ± 0.26 3.76 ± 0.28 0.23 HDL, mmol / l 0.97 ± 0.09 * 1.26 ± 0.11 * 1.31 ± 0.1 0.02 Index 4.71 ± 0.6 * 3.22 ± 0.26 3.48 ± 0.36 0.002 atherogenicity 1.09 ± 0.07 1.42 ± 0.06 * 1.12 ± 10.0 0.008 OSA, mmol / l 333.6 ± 38.5 370.9 ± 41.9 328.5 ± 37.2 0.15 USP, micromol / l Note: (*) - significance level <0.05 between subgroup B after corrective measures and control group.

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Claims (1)

A method for the nutritional prevention and treatment of atherosclerosis by the introduction of vitamin-antioxidant dietary supplements, characterized in that Pharmanex dietary supplements are introduced: Life Pack 1 tavliot in the morning and evening, Marina Omega® 1 capsule in the morning; "T-green ⁹⁷ ", 1 capsule in the morning every other day for 8 weeks.