RU2304974C2 - Method for preparing antibacterial wound-healing agent - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves carrying out immobilization of protein on methylcellulose used as a high-molecular compound and modification of its surface. Goat native milk casein prepared by flocculation is immobilized on its surface at temperature 50°C. Then silver nitrate and fucoidan are added in the ratio of components providing the following ratio of components in the agent, wt.-%: goat native casein, 40-45; methylcellulose, 40-45; silver nitrate, 0.5-2.5, and fucoidan, 9.5-17.5. Invention provides expanding antibacterial and wound-healing agents.

EFFECT: improved preparing method of agent.

7 cl, 1 tbl, 3 ex

Description

The invention relates to medicine, in particular to the pharmaceutical industry, and is intended to produce an antibacterial wound healing agent.

A known method of obtaining a therapeutic agent (patent RU 2172180 C1, 08.20.01), which uses silver nitrate, aluminum hydroxide gel, polyethylene glycol, interferon. The disadvantages are the technical complexity of the method, the use of expensive components.

The closest to the proposed technical essence and the achieved positive effect is a method of manufacturing a cosmetic product (patent RU 2146516 C1, 03.20.00.), Which contains colloidal silver stabilized with protein in deionized water using dimethyl sulfoxide. The disadvantage is that the resulting system does not provide acceleration of wound healing.

The purpose of the invention is the development of a new low-cost antibacterial wound healing agent.

To do this, in the proposed method for producing an antibacterial wound healing agent, providing for the immobilization of a protein on a high molecular weight carrier, followed by the introduction of an antimicrobial agent and a wound healing additive, native goat casein is used as a protein, which is applied to methylcellulose at a temperature of 50 ° C in a ratio of 1 ÷ 1, in Silver nitrate is used as an antimicrobial agent and fucoidan alcohol solution is used as a wound healing additive.

A method of obtaining an antibacterial wound healing agent is as follows.

Methyl cellulose is introduced into warm water with a temperature of 50 ÷ 60 ° C until a 1-1.5% solution is obtained. The mixture is kept for 1.5-2 hours. The temperature regime is due to the following restrictions. At temperatures below 50 ° C and above 60 ° C, the gelation process takes longer.

The obtained colloidal gel of methyl cellulose is introduced into goat milk, pre-fat-free and heated to a temperature of 40 ÷ 45 ° C, for better separation, in a ratio of 1 ÷ 1 by weight, followed by gradual cooling for 2-3 hours to a temperature of 10 ÷ 15 ° C and thermostating at 10 ÷ 15 ° C for 8 ÷ 10 hours. When introducing a solution of methyl cellulose in a smaller amount, casein does not completely precipitate, while increasing the mass fraction of the solution of methyl cellulose, the yield of casein remains unchanged, and the component consumption increases. Separation of protein and polysaccharide at a temperature below 10 ° C and above 15 ° C is slower.

This method of concentrating casein is based on two physicochemical phenomena: the limited thermodynamic compatibility of proteins and polysaccharides in water, as well as a higher osmotic pressure of a polysaccharide solution compared to the osmotic pressure of a protein solution. Mixing solutions of protein and polysaccharide in certain concentrations leads to the formation of a two-phase system: the lower phase is a casein concentrate, and the upper is a polysaccharide phase. Through the interface, water is transferred from the protein solution to the polysaccharide solution until phase equilibrium is established, which causes the concentration of casein.

The bottom layer containing casein is separated on a separatory funnel.

Next, prepare a 4-5% solution of methylcellulose, introducing it into warm water with a temperature of 50 ÷ 60 ° C. The mixture is kept for 1.5-2 hours. The temperature regime is due to the following restrictions. At temperatures below 50 ° C and above 60 ° C, the gelation process takes longer.

Native goat casein and a 4-5% methyl cellulose solution are introduced into a chemical reactor with a casein-methyl cellulose component ratio of 1 ÷ 1 at a temperature of 50 ° C, where the components are continuously mixed. After 30 minutes, silver nitrate in an amount of 0.5-2.5% by weight and an alcoholic solution of fucoidan in an amount of 9.5-17.5% by weight are introduced into the same reactor.

The antibacterial wound healing agent can be used in gel form, in the form of cotton-gauze napkins and in sublimated form.

The final product is autoclaved and packaged.

The finished product is a thick, homogeneous, odorless, greenish-brown mass.

The proposed method for producing an antibacterial wound healing agent is illustrated by specific examples of its manufacture, tabulated.

Table The composition of the funds Examples one 2 3 Native goat casein 40 42 45 Cellulose 40 42 45 Silver nitrate 2.5 1.75 0.5 Fucoidan 17.5 14.25 9.5

Example 1. Native goat casein is mixed with a 4-5% solution of methyl cellulose with a ratio of casein ÷ methyl cellulose 1 ÷ 1 at a temperature of 50 ° C. The resulting mixture was stirred for 30 minutes. Next, silver nitrate is added in an amount of 2.5% by weight and a solution of fucoidans in an amount of 17.5% by weight. The final product is autoclaved.

It is used in gel form, the advantage of which over other forms used for the same purpose is that it is well applied to the skin, the wound surface, is well distributed and easily washed off with water, without damaging the tissue.

Example 2. Carried out analogously to example 1, except that these components are taken in the following ratio, wt.%:

Native goat casein 42 Cellulose 42 Silver nitrate 1.75 Fucoidan 14.25

Next, the obtained gel is impregnated with multilayer gauze napkins of various sizes (from 5 × 5 cm to 30 × 30 cm). The final product is autoclaved. Napkins have high stability and duration of action, atraumatic. The use of napkins reduces the duration of treatment. The consumption of drugs and dressings for the course of treatment is reduced, the work of medical personnel is facilitated. It is especially convenient to use wipes in the field, when eliminating the consequences of natural and man-made disasters and mass casualties.

Example 3. It is carried out analogously to example 1, except that these components are taken in the following ratio, wt.%:

Native goat casein 45 Cellulose 45 Silver nitrate 0.5 Fucoidan 9.5

Next, the resulting gel is dried using sublimation. The final product is autoclaved and dried. The powder is an application sorbent that extracts toxic products of tissue degradation, microbial cells and their toxins from the surface of the affected area, which reduces the toxic load on the detoxification system of the body. This form is indicated for local use to people of any age for the prevention and treatment of inflammatory skin processes, rashes of acne, diaper rash, with weeping eczema, various dermatoses, trophic ulcers complicated by a secondary infection. Apply topically in the form of dusting, dusting powder.

The introduction of an IUD gives the agent plasticity and, due to the formation of a film upon application to the skin, increases the prolonging effect of this agent. The introduction of casein provides effective drainage in wet wounds, and the formation of the porous structure of the obtained product facilitates the access of oxygen to the wound and accelerates its healing. Fucoidan accelerates the process of wound epithelization, and silver nitrate acts as an antibacterial agent and ensures stabilization of the agent from microbiological infection.

Apply the product in a thin layer on the affected area of the skin 2-3 times a day for several days.

Claims (7)

1. A method of obtaining an antibacterial wound healing agent, characterized in that the protein is immobilized on a high molecular weight compound (IUD), surface modification, and methyl cellulose is used as a IUD, on which goat milk casein obtained as a result of flocculation is immobilized, followed by the addition of a salt of nitric acid silver and an alcoholic solution of fucoidan with a ratio of components providing the following ratio of components of the agent, wt.%: Native goat casein 40-45; Cellulose 40-45; Nitric Acid Silver 0.5-2.5; Fucoidan 9.5-17.5. 2. The method according to claim 1, in which a 4-5% solution of methyl cellulose grade MTs-100 is used as an IUD, on which goat milk casein is immobilized at a temperature of 50 ° C. 3. The method according to claim 1, characterized in that casein is used, which is isolated from skim goat milk by flocculation, using as a flocculant a solution of methyl cellulose 1-1.5 wt.% At a ratio of skim milk solution of methyl cellulose 1 ÷ 1. 4. The method according to claim 1, characterized in that flocculation is carried out by mixing the components, pre-heating goat milk to a temperature of 40-45 ° C, followed by gradual cooling for 2 hours to a temperature of 10-15 ° C. 5. The method according to claim 1, characterized in that nitric acid silver is used as an antibacterial agent in an amount of 0.5-2.5 wt.%. 6. The method according to claim 1, characterized in that used as a wound healing agent fucoidan is introduced in an amount of 9.5-17.5 wt.%. 7. The method according to claim 1, characterized in that the antibacterial wound healing agent can be used in freeze-dried form, gel-like form and in the form of cotton-gauze napkins.