RU2012122391A

RU2012122391A - METHOD FOR PRODUCING INTEGRATED FODDER PRODUCT WITH LYSIN PRODUCER BASED ON Corynebacterium glutamicum

Info

Publication number: RU2012122391A
Application number: RU2012122391/10A
Authority: RU
Inventors: Валерий Геннадьевич Правдин; Любовь Захарьевна Кравцова; Нина Александровна Ушакова
Priority date: 2012-05-31
Filing date: 2012-05-31
Publication date: 2013-12-10
Also published as: RU2499829C1

Abstract

Способ получения комплексного кормового препарата для сельскохозяйственных животных, птицы и рыбы, характеризующийся тем, что получают жидкую культуру штамма Corynebacterium glutamicum ВКПМ В-1959 - продуцента лизина глубинным способом при аэрации на питательной среде, содержащей, г/л: меласса - 250,0, кукурузный экстракт - 80,0, KHPO- 1,0, NHCl - 20,0, NHHPO- 10,0, мел - 20,0, вода - остальное и жидкие культуры штаммов Bacillus subtilis ВКПМ В-8130, Bacillus subtilis ВКПМ В -2984, Bacillus subtilis ВКПМ В-4099 и Bacillus licheniformis ВКПМ В-4162, затем 30 л жидкой культуры штамма Corynebacterium glutamicum ВКПМ В-1959 добавляют к 35 л смеси, состоящей из жидких культур Bacillus subtilis ВКПМ В-8130, Bacillus subtilis ВКПМ В-2984 и Bacillus subtilis ВКПМ В-4099, взятых в соотношении 6:6:1, соответственно, и полученную общую смесь жидких культур в количестве 65 л наносят на 200 кг предварительно подготовленного носителя - стерильного свекловичного жома, обработанного целлюлолитическим ферментом и обогащенного ферментолизатом кормовых дрожжей Saccharomyces cerevisiae, для получения которого готовят: мелассу - 255-265 г, калий фосфорнокислый двузамещенный - 98-102 г, магний сернокислый - 25-26 г, дрожжи Saccharomyces cerevisiae - 3 кг и воду до 30 л, смесь стерилизуют при температуре 120°С в течение 30 мин и добавляют к носителю, в который вносят раствор целловиридина или целлюлокса F с содержанием целлюлазы не менее 2000 ед/г из расчета 200 г на общий объем жидкости доводят рН до 6,0-6,5, смесь тщательно перемешивают, выдерживают 2 ч при температуре 45-50°С, и проводят твердофазную ферментацию в условиях ограниченного доступа кислорода при температуре 37-50°С, рН 7,5-8,0 и влажности замеса 43-48% в течение 48-50 ч, в полученный продукт добавляют 65 л жидкой культуры Bacillus licheniformis ВКПМ В-4162, содержащей не менее 5,6х 108 КA method of obtaining a comprehensive feed preparation for farm animals, poultry and fish, characterized in that a liquid culture of the strain Corynebacterium glutamicum VKPM B-1959 is obtained - a lysine producer by the deep method during aeration on a nutrient medium containing, g / l: molasses - 250.0, corn extract - 80.0, KHPO - 1.0, NHCl - 20.0, NHHPO - 10.0, chalk - 20.0, water - the rest and liquid cultures of Bacillus subtilis strains VKPM B-8130, Bacillus subtilis VKPM B - 2984, Bacillus subtilis VKPM B-4099 and Bacillus licheniformis VKPM B-4162, then 30 l of the liquid culture of the strain Corynebacterium glutamicum VKPM B-1959 is added to 35 l of the mixture, with consisting of liquid cultures of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984 and Bacillus subtilis VKPM B-4099 taken in a ratio of 6: 6: 1, respectively, and the resulting total liquid culture mixture in an amount of 65 l is applied to 200 kg a pre-prepared carrier - sterile beet pulp, treated with a cellulolytic enzyme and enriched with fermentolizate of Saccharomyces cerevisiae fodder yeast, for which preparation: molasses - 255-265 g, disubstituted potassium phosphate - 98-102 g, magnesium sulfate - 25-26 g, Sacchar yeast cerevisiae - 3 kg and water up to 30 l, cm l sterilized at a temperature of 120 ° C for 30 minutes and added to a carrier into which a solution of celloviridine or cellulox F is added with a cellulase content of at least 2000 units / g, based on 200 g, the pH is adjusted to a total volume of 6.0-6, 5, the mixture is thoroughly mixed, incubated for 2 hours at a temperature of 45-50 ° C, and solid phase fermentation is carried out under conditions of limited access of oxygen at a temperature of 37-50 ° C, pH 7.5-8.0 and a kneading humidity of 43-48% within 48-50 hours, 65 l of Bacillus licheniformis VKPM B-4162 liquid culture containing at least 5.6 x 108 K are added to the resulting product

Claims

A method of obtaining a comprehensive feed preparation for farm animals, poultry and fish, characterized in that a liquid culture of the strain Corynebacterium glutamicum VKPM B-1959 is obtained - a lysine producer by the deep method during aeration on a nutrient medium containing, g / l: molasses - 250.0, corn extract - 80.0, K ₂ HPO ₄ - 1.0, NH ₄ Cl - 20.0, NH ₄ HPO ₄ - 10.0, chalk - 20.0, water - the rest and liquid cultures of Bacillus subtilis strains VKPM B -8130, Bacillus subtilis VKPM B-2984, Bacillus subtilis VKPM B-4099 and Bacillus licheniformis VKPM B-4162, then 30 l of the liquid culture of the strain Corynebacterium glutamicum VKPM B-1959 is added to 35 l a mixture consisting of liquid cultures of Bacillus subtilis VKPM B-8130, Bacillus subtilis VKPM B-2984 and Bacillus subtilis VKPM B-4099 taken in a ratio of 6: 6: 1, respectively, and the resulting total liquid culture mixture in an amount of 65 l is applied to 200 kg of a pre-prepared carrier - sterile beet pulp, treated with a cellulolytic enzyme and enriched with fermentolizate of Saccharomyces cerevisiae fodder yeast, for which preparation: molasses - 255-265 g, disubstituted potassium phosphate - 98-102 g, magnesium sulfate - 25-26 g, Saccharomyces cerevisiae yeast - 3 kg and water until 30 l, the mixture is sterilized at a temperature of 120 ° C for 30 minutes and added to a carrier into which a solution of celloviridine or cellulox F is added with a cellulase content of at least 2000 units / g at a rate of 200 g, the pH is adjusted to a total volume of 6.0 -6.5, the mixture is thoroughly mixed, incubated for 2 hours at a temperature of 45-50 ° C, and solid-phase fermentation is carried out under conditions of limited access of oxygen at a temperature of 37-50 ° C, pH 7.5-8.0 and a kneading humidity of 43- 48% within 48-50 hours, 65 L of Bacillus licheniformis VKPM B-4162 liquid culture containing at least e 5.6 x 108 CFU / g, the mixture is thoroughly mixed and dried to a moisture content of 8-10%, after which dry powders of the herb Echinacea purpurea and milk thistle are added, respectively, at the rate of 20-50 g per 1 kg of the final product, the resulting mixture mix and crush until a homogeneous mass.