RU2012120854A - Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот - Google Patents
Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот Download PDFInfo
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Abstract
1. Неприродный белок с цинковыми пальцами, модулирующий экспрессию по меньшей мере одного эндогенного гена растения, где по меньшей мере один ген растения вовлечен в биосинтез жирных кислот и выбран из группы, состоящей из гена β-кетоацил-ACP-синтетаза (KAS), гена тиоэстеразы жирных кислот B (FatB), гена синтазы жирных кислот (FAS), гена элонгазы жирных кислот, гена тиоэстеразы жирных кислот A (FatA), гена G-3-P-дегидрогеназы пластид (GPDH), гена глицерокиназы (GK), гена десатуразы белка-носителя стеароилацила (S-ACP-DES) и гена олеоил-ACP-гидролазы.2. Слитый белок, содержащий белок с цинковыми пальцами по п.1 и функциональный домен.3. Слитый белок по п.2, где функциональный домен представляет собой домен регуляции транскрипции или домен расщепления.4. Белок с цинковыми пальцами или слитый белок по любому из пп.1-3, где ген растения содержится в растении.5. Белок с цинковыми пальцами по п.4, где белок с цинковыми пальцами связывается с участком-мишенью, как представлено в таблице 2 или таблице 10A.6. Белок с цинковыми пальцами по п.4, где белок с цинковыми пальцами содержит области распознающих спиралей, представленные в строках таблицы 1 или таблицы 10B.7. Полинуклеотид, кодирующий один или несколько белков с цинковыми пальцами или слитых белков по любому из пп.1-6.8. Способ модификации одного или нескольких генов, вовлеченных в биосинтез жирных кислот в растительной клетке, где способ включает:введение в растительную клетку одного или нескольких экспрессирующих векторов, содержащих по меньшей мере один полинуклеотид по п.7, в таких условиях, что экспрессируются один или несколько белков с цинковыми пальцами, и подвергаются модификации один или нескольк�
Claims (19)
1. Неприродный белок с цинковыми пальцами, модулирующий экспрессию по меньшей мере одного эндогенного гена растения, где по меньшей мере один ген растения вовлечен в биосинтез жирных кислот и выбран из группы, состоящей из гена β-кетоацил-ACP-синтетаза (KAS), гена тиоэстеразы жирных кислот B (FatB), гена синтазы жирных кислот (FAS), гена элонгазы жирных кислот, гена тиоэстеразы жирных кислот A (FatA), гена G-3-P-дегидрогеназы пластид (GPDH), гена глицерокиназы (GK), гена десатуразы белка-носителя стеароилацила (S-ACP-DES) и гена олеоил-ACP-гидролазы.
2. Слитый белок, содержащий белок с цинковыми пальцами по п.1 и функциональный домен.
3. Слитый белок по п.2, где функциональный домен представляет собой домен регуляции транскрипции или домен расщепления.
4. Белок с цинковыми пальцами или слитый белок по любому из пп.1-3, где ген растения содержится в растении Brassica.
5. Белок с цинковыми пальцами по п.4, где белок с цинковыми пальцами связывается с участком-мишенью, как представлено в таблице 2 или таблице 10A.
6. Белок с цинковыми пальцами по п.4, где белок с цинковыми пальцами содержит области распознающих спиралей, представленные в строках таблицы 1 или таблицы 10B.
7. Полинуклеотид, кодирующий один или несколько белков с цинковыми пальцами или слитых белков по любому из пп.1-6.
8. Способ модификации одного или нескольких генов, вовлеченных в биосинтез жирных кислот в растительной клетке, где способ включает:
введение в растительную клетку одного или нескольких экспрессирующих векторов, содержащих по меньшей мере один полинуклеотид по п.7, в таких условиях, что экспрессируются один или несколько белков с цинковыми пальцами, и подвергаются модификации один или несколько генов.
9. Способ по п.8, где модификация включает модуляцию экспрессии по меньшей мере одного гена.
10. Способ по п.9, где активирована экспрессия по меньшей мере одного гена.
11. Способ по п.9 или 10, где репрессирована экспрессия по меньшей мере одного гена.
12. Способ по п.8, где полинуклеотид кодирует нуклеазы с цинковыми пальцами и расщепляется по меньшей мере один ген.
13. Способ по п.12, дополнительно включающий этап введения донорной нуклеиновой кислоты так, что донорный вектор посредством гомологичной рекомбинации встраивается в участок расщепления.
14. Растительная клетка, содержащая по меньшей мере один ген, модифицированный любым из способов по любому из пп.8-13.
15. Растительная клетка по п.14, где клетка находится в семени и содержание жирных кислот в семени модифицировано.
16. Растение, содержащее по меньшей мере одну клетку по любому из пп.14 и 15.
17. Семена или потомство растения по п.16.
18. Растение, содержащее по меньшей мере одну клетку, содержащую белок с цинковыми пальцами по пп.1-6 или полинуклеотид по п.7.
19. Семена или потомство растения по п.18.
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US27952809P | 2009-10-22 | 2009-10-22 | |
US61/279,528 | 2009-10-22 | ||
PCT/US2010/002817 WO2011049627A1 (en) | 2009-10-22 | 2010-10-22 | Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis |
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RU2015111840A Division RU2712833C2 (ru) | 2009-10-22 | 2010-10-22 | Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот |
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RU2015111840A RU2712833C2 (ru) | 2009-10-22 | 2010-10-22 | Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот |
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US (3) | US8592645B2 (ru) |
EP (2) | EP2722392B1 (ru) |
JP (1) | JP5827233B2 (ru) |
KR (1) | KR101813981B1 (ru) |
CN (2) | CN102782140B (ru) |
AR (1) | AR078740A1 (ru) |
AU (1) | AU2010308569B2 (ru) |
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NZ (2) | NZ619018A (ru) |
RU (2) | RU2568056C2 (ru) |
UA (2) | UA120742C2 (ru) |
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