RU2002122179A - A method of preparing a purified preparation of botulinum toxin type A - Google Patents

A method of preparing a purified preparation of botulinum toxin type A

Info

Publication number
RU2002122179A
RU2002122179A RU2002122179/13A RU2002122179A RU2002122179A RU 2002122179 A RU2002122179 A RU 2002122179A RU 2002122179/13 A RU2002122179/13 A RU 2002122179/13A RU 2002122179 A RU2002122179 A RU 2002122179A RU 2002122179 A RU2002122179 A RU 2002122179A
Authority
RU
Russia
Prior art keywords
toxin
preparing
purified preparation
exchange chromatography
toxin type
Prior art date
Application number
RU2002122179/13A
Other languages
Russian (ru)
Other versions
RU2230325C2 (en
Inventor
Олег Евгеньевич Грачев
Георгий Дмитриевич Елагин
Михаил Юрьевич Дубровин
Людмила Владимировна Бакулина
Борис Анатольевич Рудой
тков Владимир Анатольевич П
Владимир Анатольевич Пятков
Леонид Михайлович Телицын
Original Assignee
Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации filed Critical Научно-исследовательский институт микробиологии Министерства обороны Российской Федерации
Priority to RU2002122179/13A priority Critical patent/RU2230325C2/en
Publication of RU2002122179A publication Critical patent/RU2002122179A/en
Application granted granted Critical
Publication of RU2230325C2 publication Critical patent/RU2230325C2/en

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Claims (1)

Способ приготовления очищенного препарата ботулинического токсина типа А, включающий культивирование штамма-продуцента Cl. Botulinum в жидкой питательной среде, отделение токсинсодержащих компонентов из культуральной жидкости, кислотно-солевое осаждение белков из надосадочной жидкости, экстракцию токсина из осадка, фракционирование компонентов препарата методами гельфильтрации и анионообменной хроматографии, отличающийся тем, что перед этапом хроматографии белки из экстракта токсина двукратно осаждают в присутствии сульфата аммония до степени насыщения 20% и 40% соответственно, гельфильтрацию и анионообменную хроматографию выполняют в условиях, оптимальных для диссоциации компонентов токсического комплекса при рН 7,9 и концентрации соли фосфорнокислого натрия 0,025 М, адсорбционную хроматографию выполняют перед этапом анионообменной хроматографии, а в качестве адсорбента на этапе адсорбционной хроматографии используют гидроксиапатит.A method of preparing a purified preparation of botulinum toxin type A, comprising cultivating a producer strain of Cl. Botulinum in a liquid nutrient medium, separation of toxin-containing components from the culture fluid, acid-salt precipitation of proteins from the supernatant, extraction of toxin from the precipitate, fractionation of the drug components by gel filtration and anion-exchange chromatography, characterized in that prior to the chromatography step, proteins from the toxin extract are twice precipitated into the presence of ammonium sulfate to a degree of saturation of 20% and 40%, respectively, gel filtration and anion exchange chromatography are performed under optimal conditions to dissociate the components of the toxic complex at pH 7.9 and a sodium phosphate salt concentration of 0.025 M, adsorption chromatography is performed before the anion exchange chromatography step, and hydroxyapatite is used as the adsorbent in the adsorption chromatography step.
RU2002122179/13A 2002-08-15 2002-08-15 Method for preparing purified preparation of botulinic toxin type a RU2230325C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2002122179/13A RU2230325C2 (en) 2002-08-15 2002-08-15 Method for preparing purified preparation of botulinic toxin type a

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002122179/13A RU2230325C2 (en) 2002-08-15 2002-08-15 Method for preparing purified preparation of botulinic toxin type a

Publications (2)

Publication Number Publication Date
RU2002122179A true RU2002122179A (en) 2004-02-20
RU2230325C2 RU2230325C2 (en) 2004-06-10

Family

ID=32845984

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002122179/13A RU2230325C2 (en) 2002-08-15 2002-08-15 Method for preparing purified preparation of botulinic toxin type a

Country Status (1)

Country Link
RU (1) RU2230325C2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2255761C1 (en) * 2004-07-01 2005-07-10 Общество с ограниченной ответственностью "Токсины и сопутствующие продукты" Preparation for treatment of muscular dystonia from toxin of culture clostridium botulinum and method for its preparing
ES2524312T3 (en) * 2008-03-14 2014-12-05 Allergan, Inc. Assays of immunotype-based botulinum toxin serotype A activity
JP5795539B2 (en) * 2009-02-19 2015-10-14 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー Means and methods for producing high purity neurotoxins
HUE047067T2 (en) * 2009-03-13 2020-04-28 Allergan Inc Immuno-based retargeted endopeptidase activity assays
US8129139B2 (en) * 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
KR101729251B1 (en) * 2015-04-28 2017-04-21 주식회사 대웅 Medium Composition for Preparing Botulinum Toxin

Also Published As

Publication number Publication date
RU2230325C2 (en) 2004-06-10

Similar Documents

Publication Publication Date Title
ES2643554T3 (en) Process and system to obtain botulinum neurotoxin
CN102875669B (en) Method for separating and extracting ovotransferrin
ATE314391T1 (en) METHOD FOR PURIFYING THROMBOPOIETIN
RU2002122179A (en) A method of preparing a purified preparation of botulinum toxin type A
CN103497248A (en) Method for separating and purifying antibody from cell culture supernatant
CN104292316A (en) Efficient separation and purification method of Gracilaria verrucosa reagent grade R-phycoerythrin
US4743551A (en) Purification of microbial rennet from Mucor miehei
JP7454507B2 (en) Method for producing botulinum toxin
CN101747409A (en) Cobra-venom factor and cobra-venom neurotoxin combined separation and purification method
CN1243015C (en) Separating and purifying technology for micro algae phycoerythrin
CN1128220C (en) Process for separating worm kinase
CN1250567C (en) Method of purifying calcium ion-binding protein
CN102993267A (en) Preparation method of glossy ganoderma bioactive peptide
CN103694334B (en) A kind of method preparing hEGF raw product
CN102660525B (en) Method for preparing human urinary kallidinogenase crude product
RU2003106162A (en) METHOD FOR PRODUCING COLLAGENASE PRODUCT
SU535912A3 (en) Method of producing orgotein
SU508509A1 (en) Method for isolating enzymes of microbial bee mass
RU2225441C1 (en) Method for preparing collagenase preparation
SU1377292A1 (en) Method of cleaning proteinases of cysteic type
EP4368635A1 (en) Method for purification of clostridium botulinum neurotoxin protein deprived of non-toxin protein
CN100548314C (en) Snake venom cytotoxin and preparation method thereof and application
CN1597943A (en) Process for extracting oxalate oxidase from bran of wheat
RU2065876C1 (en) Process for recovering enzyme agent from muscle tissue of fish
JP4670077B2 (en) Method for purifying bioactive polymer substance and purified product obtained by the method

Legal Events

Date Code Title Description
MM4A The patent is invalid due to non-payment of fees

Effective date: 20070816