RU134422U1 - DEVICE FOR MODELING A LOCAL BURN OF THE ESOPHAGULA IN EXPERIMENTAL ANIMALS - Google Patents

Abstract

A device for modeling a local burn of the esophagus in experimental animals, containing a probe in the form of a tube made of elastic material with a side opening, characterized in that a capillary is inserted inside the probe, the distal end of the round-shaped probe blindly ends, and a tampon is located in the area of the distal end of the probe and the side opening hygroscopic material adjacent to the capillary.

Description

The proposed device relates to medicine, namely to surgery and can be used to study the features of the formation of the post-burn scar of the esophagus wall and the effect of various medications on the healing process.

The main problems of the formation of esophageal burns in animals in the experiment are the difficulty in dosage of the chemical agent and, as a result, the high mortality of animals from shock at the stage of injury, as well as the need to create a local scar on the wall of the esophagus, preventing its circular nature. The creation of a locally burn limited in area will reduce the death of animals from exhaustion in the later stages of the experiment.

Of the most famous close in technical essence is a device for modeling an acute burn of the esophagus in the experiment, proposed by D.P. Trofimov [1], which is a polyethylene tube with several side holes and an end cap at the distal end.

However, the device for modeling a burn according to the method of D.P. Trofimov noted a number of shortcomings. When using a polyethylene tube with several side openings, the reagent comes in contact with a large surface of the esophagus, which in turn leads to a larger burn area and an increase in mortality of animals. The lack of dosed exposure to the reagent with the surface of the esophagus due to the uneven distribution of the corrosive solution over the organ wall leads to the appearance of areas with different depths of damage, which also increases the lethality of animals.

The technical result of the proposed device is the creation of a device that provides a local, small area burn of the esophagus.

The essence of the proposed method is that for modeling a local burn, according to the proposed utility model, a probe with a capillary inserted into its lumen is used as a delivery device for a chemical agent. Distinctive features of the device is the presence of one side hole on the probe to delimit the burn area. A capillary is a means of delivering a chemical agent directly to the probe opening, which allows you to control the volume of the injected solution. As a result, the chemical agent acts only through one hole in a constant volume, dosing the chemical agent, which will allow the formation of a local burn. At the distal end, there is a gauze swab inside the probe necessary to reduce the feed rate of the chemical.

The proposed device for modeling a burn of the esophagus is illustrated by drawings.

Figure 1 shows a probe - a polyethylene tube with a capillary in the lumen 3 with a side hole 1, blindly ending with the end 2, with a gauze swab 5. The proximal end 4 is free.

The probe was used as follows. The experiment was carried out in accordance with the order of the Ministry of Health of the USSR dated 12.08.1977 No. 755 "On measures to further improve the organizational forms of work using experimental animals." Under general anesthesia, the probe was inserted into the esophagus to the level of the middle third. To create a burn through the capillary, a cauterizing liquid was introduced through the probe, wetting the swab, with its further exposure for a certain time. Subsequently, the probe was quickly removed. The animal was withdrawn from anesthesia.

Example.

A burn was created by exposing acetic acid for 1 minute, in a volume of 1 ml. To do this, under general anesthesia, the oral cavity was expanded to a rabbit with a child’s mouth expander, an 8 mm diameter probe was inserted with a 5 × 2 mm lateral opening near the blindly terminating end with a gauze swab inside, a capillary was inserted into the probe lumen to the level of the lateral opening of the probe. The length of the probe inserted into the esophagus should be 12-15 cm, which corresponds to the level of the middle third of the rabbit’s esophagus [2]. Initially, 0.5 ml was introduced per capillary. 46% acetic acid so that the liquid reaches the distal opening of the capillary, then 1 ml is dispensed. 46% acetic acid. The exposure time was 1 min. Subsequently, the probe was quickly removed. To mitigate the side effects of anesthesia and rabbit awakening, caffeine benzoate was introduced in oil of 1 ml of 10% solution. For 30 minutes, the state of the animals was monitored prior to recovery from anesthesia.

Information sources:

1. Trofimov D.P. Prevention of the formation of post-burn cicatricial stenosis of the esophagus (experimental clinical study): dis. ... cand. honey. sciences. - Voronezh, 2004 .-- 146 p.

2. Popesco P. Atlas of the anatomy of domestic animals. Bratislava .: Nature, 1974.- v.2 - 187 p.

Claims (1)

A device for simulating a local burn of the esophagus in experimental animals, containing a probe in the form of a tube of elastic material with a side opening, characterized in that a capillary is inserted inside the probe, the distal end of the round probe is blindly terminated, and a tampon is located in the area of the distal end of the probe and the side opening hygroscopic material adjacent to the capillary.

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