PL154810B1 - Method for full utilization of oil-containing fuller's earth from the oil industry - Google Patents

Description

REPUBLIC POLAND PATENT DESCRIPTION 154 810 Additional patent to patent no --- Reported: 88 and 23 / P. 275974 / Int. Cl.s A23K 1/00 C12N 1/16 Priority-- szmiNU B 6 Ó L S I OFFICE PATENT Application announced: 89 07 24 RP Patent description published: 1992 01 31 -

Invention: Marek Kramarz

The holder of the patent: Akademia Ekonomiczna im. Oskar Lange, Wrocław / Poland, /

METHOD OF BIALCO-OITAHIN-MINERAL FEED MIXTURE

The subject of the invention is a method of producing a protein-vitamin-mineral feed mixture from waste obtained in the fat industry - oily bleaching earth.

About the fat industry, large amounts of oily bleaching earth containing from 30 to 50% of fatty substances are obtained in the process of oil decolorization. There are known methods of recovering fat from bleaching earth by blowing with air at a temperature of 20-30 ° C for 1 hour, blowing with steam at a pressure of 2-3 at. and a temperature of 120-130 ° C for 1 hour, extraction with hot water at a pressure of 3-4 at. and a temperature of 9O-95 ° C for 1 hour and extraction with organic solvents, e.g. gasoline or hexane at a pressure of 1-2 at. at 50-60 ° C for 1 hour. After the purging and extraction process 45 to 10% of the original oil content remains in the bleaching earth.

There are also known methods of recovering oil from bleaching earth by autoclaving a mixture containing 1/2 volume of soil and / 2 volume of water with the addition of 3% NaCl and 3% 9ody. A pressure of 3 at is used. within 3 hours. After this time, the whole thing is left to stand. The effectiveness of the method is 75%.

The disadvantage of these methods is incomplete recovery of fat from bleaching earth and the need to store partially oily earth as waste.

There are known methods of producing vitamin-mineral feed mixtures or mineral-vitamin-antibiotic preparations which will supplement industrial or farm fodder. These mixtures, produced in Poland by the pharmaceutical industry, are obtained by combining vitamins, minerals, antibiotics and sometimes amino acids, depending on the intended use, by simply mixing the ingredients in a suitable manner. Each of the above-mentioned components of the mixture is obtained separately, e.g. vitamins, amino acids and antibiotics are obtained as a result of the cultivation of special, high-yielding particulars.

154 810 of microorganisms and the complicated treatment of the substrate with biomass to isolate them in the form of a preparation.

The disadvantage of the described method of obtaining compound feed is the high production cost, the complicated technological process and the incomplete chemical composition in relation to the nutritional needs.

The method according to the invention consists in inoculating a suspension containing 2 to 20% by weight of oily bleaching earth in an aqueous synthetic medium with a known composition necessary for yeast growth, inoculated with an active culture of fodder yeast, for example of the species Trichosporon cutaneim, Kluyveromyces fragilis, Candida utilis, Crndida pseudotropicaais, Saccharomyces formoii ^ nsiss, followed by the known aerobic cultivation process. Then the biomass is separated together with the diatomaceous earth and dried. The liquid after biomass separation is used to prepare an aqueous suspension of diatomaceous earth.

The method according to the invention makes it possible to obtain protein-vitamin <polynwo-mine: oal feed mixture characterized by a high content of protein, B vitamins, vitamins D and F and such inorganic ingredients as: silica, magnesium, sodium, potassium, calcium for feeding poultry 1 pig. The use of bleaching earth is complete, without waste and litter, and eliminates the need to store it as a waste product.

EXAMPLE 1 For the preparation of Triahtsptrtn cutan and mum yeast with high, native emulsifying activity, cultivation was carried out on a synthetic medium containing 2% crude rapeseed oil. After 10 hours culture H resulted in 31.8 g dry weight of β

yeast in 1 dm of medium. The whole thing was divided into two parts. One part was centrifuged (A), separating the biomes, and the other (B-yeast fluid) was used as a seed in the performed experiment.

100 g of oily bleaching earth from the decolorization process of crude rapeseed oil, containing 45 g of fatty substances, determined as eater extract, were weighed. The soil was spread in a synthetic substrate with the composition / in 1 dm /:

/ NH _4/2 S0 ₄ - 5 g

ABOUT

H ^j P0 ₄ s ^toc . ⁸⁵ # - 2.5 cm

MfglO ^ THgO - 1.5 g

KOI - i.ii g

Then, 1000 cm of the prepared substrate was inoculated with yeast with fermentation liquid B. The process was carried out at 32 ° C, with aeration and pH of the culture environment

4.5, stabilized with 1 mol NH.OH. After 17 hours, the cultivation process was completed with a net dry biomass increase of 54.1 g / dm2. After separating the biomass and bleaching earth, the content of fatty substances in the soil was determined without their presence in the substrate.

The process yield was 120% relative to the Hpid compounds in the bleaching earth at the beginning of the H cultivation. The yeast biomass, together with the bleaching earth, after drying and mixing, can be used for feeding poultry or pigs.

Blałktio-vitamiztwo-ιineralza the feed mixture contains approx. 37% of protein in dry matter, 44% of ash, 50% of which are cancer and microelements soluble in 10% HCC, and thus absorbed by animals, 12% of intracellular lipids with a favorable composition of fatty acids . Among the vitamins there are: B group vitamins, ie B ^, B ₂ , B _g , vitamin D in the amount of 450 mg / kg of the mixture and vitamin F in the amount of 21.4% of the total Hpidis content in the mixture.

Example II. 100 g of oily bleaching earth was mixed with a substrate with a chemical composition identical to that in Example 1. Centrifuged yeast A from Example 1 was used as a seed. After 18 hours cultivation H yielded 42 g * 1 dm, corresponding to the substrate yield = 80%. The lower efficiency index was due to the lower biological activity of inoculate A / centrifuged yeast / compared to the stock inoculum in Example 1 / yeast with digestate /. As the analyzes of the post-fermentation fluid from

The yeast Trichosporon cultures have a high emulsifying activity due to the presence of exogenous blockemulsifiers. 3.5 g of unused lipids per 100 g of sample were found in the bleaching earth.

Example III. A synthetic substrate was prepared containing in 1 dm ^J : Ah _4/2 so ₄ - 3 g / nh _4/2 HPO ₄ - 5 g UgSO4-71 ^ 0 - 1 8 EC1 - 1 g

yeast autolysate - 10 cm pH of the medium was set with 1 mole of H3PO4 1 1 mole. 80 g of oily bleaching earth were added to the substrate prepared in this way and then inoculated with Kluyveromyces fragilis yeast culture, which in the last phase of propagation was multiplied on a medium containing malt wort and 1% crude rapeseed oil to produce high emulsifying activity of yeast. The seeding of the yeast was 107% of the volume of the medium. The process hodowwi p ^^ dziono ^{at 3} 0 ° C, ^pH 4.5 bringing in ¹ Getting napowi.etrzanl.u ¹⁰⁰ dm ³ / dm ³ / h.

After 20 hours hoioκil to give 51.0 g of D ₁ 00 / ^<m ^ ^A ^ l oorz ^ yl ^ ai ^ le of the substrate was complete.

The product was characterized by a high protein content (38% dry matter) with the preferred amino acid, for example: 6.2% lysine, 5.8% leucine, 3.5% tyrosine and 0.4% meeiotite.

Example IV. The composition of the culture medium was similar to that in Example 3-3, except that the addition of yeast autolysate was 20 cm per 1 liter of medium. The mixture of Saccharomyces fsrmosiens S8, Ctandida utilis and Condida p8eudoSropicali8 was used as inoculation. The propagation conditions and άο <^ ηϋ were similar to those described in Example 3, except that the pH of the culture medium was maintained at 4.7 and the temperature at Jyltsl was 32-33 ° C. The hodoidal process was carried out for 19 hours, obtaining 49.5 g Dj00 / ^^ m. AJymiiacja fat! by yeast was total. The product contained 38 ^% protein in dry MSL, 8% fat! occurring in biomass and 42% of minerals / ash / and vitamins, mainly a complex of B vitamins.

EXAMPLE 5 For a control stock on a medium containing 100 g of oily bleaching earth in a solution of nitrogen sources, phosphorus, potash and potassium, the yeast strain Cauadida 11 polytica was used, containing fats as a source of carbon and a source of carbon. After 20 hours In breeding it was found that 45% of lipid substances remained in the bleaching earth. It turned out that Cradida lipolytica does not produce a sufficient amount of a bloemulsifier, which enables cells to assimilate ilpid compounds admixed in soil micropores.

Claims (2)

Patent claims 1. Method for the production of protein-vitamin-mineral feed mixture by aerobic multiplication of biomass, characterized in that a suspension containing from 2 to 20% by weight of oily bleaching earth in an aqueous synthetic medium with known chemical composition of the tiezbi (hlyy for yeast growth, grafts is an active culture of fodder yeast, e.g. of the species Tricbsipsrsn cutaneum, Kluyversmyces fragilis, Crndida utilis, Saccharomyces formo sennie, Cmdida pse ^ doSroplcalis, followed by the known aerobic multiplication process of the biomass, then separated, for example, diatomaceous biomass together with e.g. by centrifuging and drying. 2. The method according to p. The process of claim 1, characterized in that the fluid after biomass separation is used to prepare an aqueous suspension of diatomaceous earth. 154 810 Department of Publishing of the UP RP. Circulation 100 copies Price: PLN 3,000