NZ505071A

NZ505071A - Separation of whey proteins from solution using anion exchangers

Info

Publication number: NZ505071A
Application number: NZ50507100A
Authority: NZ
Inventors: John Stephen Ayers; David Francis Elgar; Kay Patricia Palmano; Mark Pritchard; Ganugapati Vijaya Bhaskar
Original assignee: Univ Massey; New Zealand Dairy Board
Priority date: 2000-06-09
Filing date: 2000-06-09
Publication date: 2002-04-26

Abstract

A process of separating a preselected whey protein or group of whey proteins from a whey protein-containing solution, wherein the process comprises the steps: (a) contacting a why protein-containing solution with an anion exchanger at a pH level at which the preselected protein or group of proteins adsorbs to the anion exchanger, and wherein the anion exchanger, and wherein the anion exchanger comprises a water insoluble, hydrophilic, water swellable, hydroxy(C2-4)alkylated and cross-linked regenerated cellulose, derivatised with quaternary amino (QA) groups, wherein the level of substitution of the QA groups is 1.4 milli-equivalents per dry gram of anion exchanger (meq/g) or greater; and (b) recovering either or both of the following : (1) the whey protein or proteins adsorbed in step (a) and (2) the breakthrough whey protein or proteins not adsorbed in step (a).

Description

<div class="application article clearfix" id="description"> NEW ZEALAND PATENTS ACT, 1953 No: Date: COMPLETE SPECIFICATION PROCESSES FOR SEPARATION OF PROTEINS We, MASSEY UNIVERSITY, a body corporate of Palmerston North, New Zealand, and NEW ZEALAND DAIRY BOARD, a body corporation of Pastoral House, 25 The Terrace, Wellington, New Zealand do hereby declare the invention for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: - 1 - INTELLECTUAL PROPERTY OFFICE OF N.Z. - 8 DEC 2000 RECEIVED 2 PROCESSES FOR SEPARATION OF PROTEINS 5 TECHNICAL FIELD This invention relates to new processes useful for separating whey proteins from whey protein-containing solutions. 10 BACKGROUND OF THE INVENTION Anion exchangers have been used to make purified protein products from milk raw materials such as skim milk and whey. They have also been used to modify the properties of milk by the removal of whey proteins from skim 15 milk For example GB 1563990 (1980) discloses a method of preparing a mixture of whey proteins (a-lactalbumm, (3-lactoglobulin, serum albumin and some immunoglobulin), now known as whey protein isolate (WPI), by passing 20 either skim milk or milk serum (whey) through a column of silica based anion exchanger, washing the column with water and then eluting and recovering the bound protein. Immunoglobulins remaining m the column effluent were recovered by passing this effluent, at the same pH still, through a column of silica which acts as a cation exchanger and adsorbs the 25 immunoglobulins. EP 0320152 (1989) also discloses a process which contacts an anion exchanger with whey or liquid whey protein concentrate to adsorb most of the whey proteins and form an effluent rich m immunoglobulins. But m EP 30 0320152 this effluent is treated differently. It is concentrated by ultrafiltration to prepare an immunoglobulin enriched whey protein concentrate powder (WPC) Both these references refer to the difficulty of performing ion exchange 35 separation of proteins on an industrial scale because many of the known ion exchangers, particularly those based on cellulose and dextran, have weak mechanical properties which do not lend themselves to large scale use. The authors proposed using anion exchangers with better mechanical properties, 171402 vl WON * 3 particularly ones based on silica and coated with cross-linked polymer film containing quaternary ammonium functional groups, such as QMA Spherosil™ (Rhone-Poulenc). Other anion exchangers mentioned specifically were Q Sepharose™ fast flow (Pharmacia), and Q Trisacryl™ 5 (IBF) However all three of these anion exchangers suffer the disadvantage of being expensive which makes it very difficult to recover whey proteins by ion exchange industrially, for economic reasons. GB 1563990 also discloses a manner of using the anion exchanger to bring 10 about a more selective separation of the whey proteins. This is achieved by passing the milk (or whey) through two columns filled with anion exchanger and used m tandem The protein recovered from the first column was very rich in p-lactoglobulm while that recovered from the second column contained a-lactalbumin, serum albumin and small quantities of 0-15 lactoglobulin and immunoglobulins. This is a result of P-lactoglobulin binding more tightly to the anion exchanger than the other whey proteins bind. This has been well documented, particularly for this polymer coated silica based anion exchanger QMA Spherosil™. See J of Dairy Research, 52, 167-181, 1985. 20 25 WO 97/26797 describes processes similar to the above processes, but the processes described use as feedstock a whey protein containing solution (such as a UF retentate or WPC) having a high protein concentration and reduced ionic strength. ^ US 5077067 (1991) further discloses the use of any strongly basic type anion exchanger for the selective and quantitative removal of (3-lactoglobulms from starting materials containing whey proteins. The examples cite the use of anion exchangers manufactured from different matrices; a cross-linked 30 agarose matrix, Q Sepharose™ (Pharmacia); a cross-linked dextran matrix, QA Sephadex™ (Pharmacia) and a cross-linked polystyrene matrix, Duolite™ A-101 (Rohm and Haas). However none of these are suitable for large scale industrial use, Q Sepharose™ being expensive and QA Sephadex™ suffering from weak mechanical properties as mentioned above. Duolite™ A-101 35 suffers the disadvantages of most polystyrene based ion exchangers in that generally they are not suitable for use with proteins. In fact Outlinen et. al. m WO 95/19714 (Example 8) demonstrates that Duolite™ A-101 binds 171402 vl WGN * 4 neither a-lactalbumm nor P-lactoglobulin m the pH range 5-8. This is typical of the properties of polystyrene based ion exchangers. So once again the processes disclosed suffer from the lack of an anion exchanger particularly useful for an industrial scale process. 5 Outmen et al (Lebensm.-Wiss U -Technol, 29, 340-343, 1996) surveyed eleven different anion exchangers from five manufacturers. These exchangers were all quaternary ammonium derivatives of cross-linked polystyrene, matrices chosen for their mechanical and chemical stability, for 10 being macroporous and particularly for their low cost. Porosity was a major consideration as open porosity is necessary to aid protein diffusion into and out of the particles and hence important for protein binding capacity. But only one of these, Diaion™ HPA 75 (Mitsubishi Kasei Corp.) was found to have properties suitable for the selective adsorption of p-lactoglobulin from 15 whey This type of anion exchanger formed the basis of WO 95/19714 for the separation of p-lactoglobulm from whey and whey protein solutions. However even though Diaion™ HPA 75 has the right mechanical properties and cost structure (about 1/100 the cost of QMA Spherosil™) it still suffers the limitation of low protein capacity when compared to conventional, albeit 20 expensive, anion exchangers for protein adsorption. The optimum load of p- lactoglobulin found for Diaion™ HPA 75 was only 16-20 mg of protein/cm3 of exchanger (Outinen, 1996), whereas it would be advantageous to have operating capacities greater than this 25 GB 2188526 (1987) discloses the use of anion exchangers to recover a different protemaceous material from whey at pH 4-6 or more particularly at pH 4 8-5 0 At this pH most of the proteins m whey are not negatively charged and hence are not adsorbed by an anion exchanger. The protemaceous material, adsorbed at pH 4.8-5.0 and then eluted, is thought 30 to be a mixture of acidic peptides and proteins. Some of these are believed to be present in milk from which the whey is made, and others produced during the manufacture of the whey by the action of proteolytic enzymes on caseins. Many of them are highly sialylated and/or phosphoiylated peptides and proteins and they include a group of minor whey proteins known as 35 proteose-peptones. In the case of rennet and cheese whey the major peptide component of this protemaceous material is glycomacropeptide (GMP) derived from the enzyme action on kappa-casein. In the case of acid whey the protemaceous material is a mixture of acidic peptides and proteins other 171402 vl WON ' 5 than GMP GB 2188526 thus provides a process for producing GMP containing other acidic peptides and proteins as minor components (from sweet wheys) or a protemaceous material composed of acidic peptides and proteins but without GMP present (from acid wheys), these products being 5 substantially free of the whey proteins a-lactalbumm, P-lactoglobulin and serum albumin which are isolated by anion exchange at higher pH. Anion exchangers cited m the examples are QMA Spherosil™ (Rhone-Poulenc), Amberlite IRA 958 (Rohm and Haas) and QA Indion™ now known as QA GibcoCel™ (Life Technologies Ltd, Auckland, New Zealand). The Spherosil 10 suffers from being expensive as outlined earlier, the Amberlite™ IRA 958 is a macroporous acrylic based synthetic resin similar to polystyrene resins mentioned earlier m that it also has low capacity for binding proteins The QA Indion™ is an industrial grade ion exchanger based on regenerated ^ cellulose and normally suitable for protein adsorption but under the 15 particular conditions cited m GB 2188526 found by us to have low capacity for GMP (3 mg/mL). GB 2251858 (1992) also describes the preparation of K-casein glycomacropeptide (GMP) using anion exchangers but differs from GB 20 2188526 m that the anion exchanger is contacted with the milk raw material containing GMP at a pH < 4 instead of pH 4-6. In the applicants' experience this produces a GMP sub-fraction which is highly glycosylated and particularly acidic (GMP is a mixture of many different glycoforms of the macropeptide ) Anion exchangers cited in this reference are the previously 25 mentioned QMA Spherosil™ and a Sephadex™ exchanger based on cross- ^ linked dextran which is unsuitable for large scale use. Processes have thus been disclosed for using anion exchangers to prepare whey protein isolate (WPI) from skim milk or whey (GB 1563990), WPI and 30 an immunoglobulin enriched WPC from whey (EP 0320152), P-lactoglobulin and a-lactalbumin enriched protein isolates (GB 1563990), p-lactoglobulin isolate and an a-lactalbumm enriched WPC (US 5077067 and WO 95/19714) and GMP from rennet or cheese wheys or concentrates (GB 2188526 and GB 2251858) 35 In those cases where skim milk is treated with the anion exchanger at pH 6 6, it has also been disclosed that the resulting milk, depleted in whey 171402 vl WON * 6 10 protein (especially m P-lactoglobulin), has enhanced heat stability properties useful for the manufacturing of cheeses (FR 2465422, 1981) and other purposes Many different anion exchangers are available for demonstrating these processes on a laboratory scale, but few are really suitable for use m the large scale processing of dairy streams for either technical or economic reasons This might be one reason why these processes m the main have not been commercially utilised One matrix that has proved to be particularly useful in large scale separation and purification of whey proteins is regenerated cellulose which has been hydroxyalkylated and cross-linked. Ion exchangers prepared on this matrix are resistant to attrition, have good protein capacity, high flow properties 15 and are available at relatively low cost. Examples of such ion exchangers based on a hydroxypropylated and cross-linked regenerated cellulose matrix which are commercially available include the SP, CM, QA, and DEAE derivatives sold as SP GibcoCel™, CM 20 GibcoCel™, QA GibcoCel™ and DEAE GibcoCel™ respectively. These ion exchangers were previously sold under the Indion™ brand name. QA GibcoCel™ and SP GibcoCel™ having a substitution level of the QA or SP groups of up to 1.2 milli-equivalents per dry gram (meq/g) are available. SP GibcoCel™, a cation exchanger, has been widely used, but QA 25 GibcoCel™, an anion exchanger, has only enjoyed limited use industrially. Levison et al (Chimica Oggi/Chemistry Today, 41-48, Nov/Dec 1994) refers to three custom made QA celluloses with substitution levels of 0.74, 0.96 and 1 24 meq/g, and discloses that these had similar protein capacities. 30 Antal and Micko (Carbohydrate Polymers 19, 167-169, 1992) describe the optimization of the reaction of microcrystalhne cellulose with the (3-chloro-2-hydroxypropyl)tnmethyl-ammonium chloride. The maximum substitution level of quaternary ammonium groups into the cellulose that they were able 35 to obtain with this reagent was 0 94 meq/g. 171402 vl WGN * 7 The celluloses used by Levison and by Antal are not suitable for repetitive use on a large scale. With the above background m mind, it is an object of the present invention 5 to provide processes of separating whey proteins from whey protein containing solutions which will go some way towards overcoming the disadvantages of the prior art, or at least to provide the public with a useful choice 10 SUMMARY OF THE INVENTION Accordingly, in a first aspect the present invention provides a process of separating a preselected whey protein or group of whey proteins from a whey protein-containmg solution, wherein the process comprises the following 15 steps: (a) contacting a whey protein-containmg solution with an anion exchanger at a pH level at which the preselected protein or group of proteins adsorbs to the anion exchanger, and wherein the anion exchanger comprises 20 a water insoluble, hydrophilic, water swellable, hydroxy (C2-C4) alkylated and cross-linked regenerated cellulose, denvatised with quaternary amino (QA) groups, wherein the level of substitution of the QA groups is 1.4 milli-equivalents per diy gram of anion exchanger (meq/g) or greater; and 25 (b) recovering either or both of the following: (1) the whey protein or proteins adsorbed m step (a) and (2) the breakthrough whey protein or proteins not adsorbed in step (a) The whey protein-containing solution may be whole milk, skim milk, whey, 30 ultrafiltered or microfiltered milk or whey, reconstituted WPC (whey protein concentrate) or MPC (milk protein concentrate), or any other milk derived material containing whey proteins Preferably, the level of substitution of QA groups on the anion exchanger is 35 from about 1 4 to about 2 5 meq/g, more preferably from about 1.5 to about 2.5 meq/g, and most preferably from about 1.7 meq/g to about 2.5 meq/g. 171402 vl WGN * 8 Preferably, the cellulose is hydroxypropylated cross-linked regenerated cellulose, preferably in granulated or beaded form. In one embodiment of the invention, step (a) comprises contacting the anion 5 exchanger with a whey protein containing solution selected from whey, UF retentate and reconstituted WPC, at a pH of from about 5 to about 8, to selectively adsorb whey proteins other than immunoglobulins. Preferably, the adsorbed proteins are recovered from the anion exchanger to 10 produce a whey protein isolate (WPI). Optionally, the breakthrough whey proteins are recovered separately, to produce an immunoglobulin isolate (conveniently by cation exchange) or an I immunoglobulin enriched WPC 15 In another embodiment of the invention, step (a) comprises contacting the anion exchanger with a whey protein containing solution selected from whey, UF retentate and reconstituted WPC, at a pH of from about 5 to about 8, under conditions m which (3-lactoglobulin and acidic peptides and proteins 20 including, when present, glycomacropeptide (GMP) are preferentially adsorbed to the anion exchanger Preferably, the adsorbed proteins are recovered from the anion exchanger to produce a WPI. 25 | Optionally, the breakthrough whey proteins are recovered separately to produce an a-lactalbumin containing WPI (conveniently by further anion exchange), an a-lactalbumin/immunoglobulin-containing WPI (conveniently by cation exchange), or a WPC enriched in a-lactalbumm and 30 immunoglobulin. In a further embodiment of the process of the invention, step (a) comprises contacting the anion exchanger with a whey protein-containing solution selected from sweet whey, UF retentate derived from sweet whey, or 35 reconstituted WPC derived from sweet whey, at a pH of about 5 or below, to selectively adsorb glycomacropeptide (GMP). 171402 vl WGN * 9 Preferably, the anion exchanger is contacted with the whey protein containing solution at a pH of about 4.5 to about 5.0. Alternatively, if the preselected whey protein is a glycosylated subtraction of 5 GMP, a pH of about 3 0 to about 4 5 may be used. Preferably, the adsorbed proteins are recovered from the anion exchanger, to produce a GMP-contammg WPI 10 Optionally, the breakthrough whey proteins are recovered separately, to produce a GMP-depleted WPC In a further embodiment of the process of the invention, step (a) comprises contacting the anion exchanger with a whey protein-containing solution 15 selected from mineral acid whey, lactic acid whey, UF retentate derived from acid whey, or reconstituted WPC derived from acid whey, at a pH of about 5 or below, to selectively adsorb acidic peptides and proteins. Preferably, the anion exchanger is contacted with the whey protein 20 containing solution at a pH of about 3 to about 5.0. Preferably, the adsorbed proteins are recovered from the anion exchanger, to produce a WPI containing acidic peptides and proteins substantially free of the major whey proteins 25 Optionally, the breakthrough whey proteins are recovered separately, to produce a WPC depleted m acidic peptides and proteins. In yet a further embodiment of the process of the invention, step (a) 30 comprises contacting the anion exchanger with a whey protein-containing solution selected from whole milk, skim milk or ultrafiltered milk, to adsorb proteins m the milk or milk retentate. Preferably, the anion exchanger is contacted with the whey protein-35 containing solution at a pH of from about 6-7, more preferably at about pH 6 6. 171402 vl WGN ' 10 Preferably, the adsorbed proteins are recovered from the anion exchanger to produce a WPI. Optionally, the breakthrough from the anion exchanger is recovered 5 separately, to produce a whey protein reduced milk, skim milk, retentate or milk protein concentrate In a further aspect, the invention also provides a whey-protein containing product prepared by a process as defined above. 10 While the present invention is broadly as defined above, it is not limited thereto and also includes embodiments of which the following description provides examples. 15 BRIEF DESCRIPTION OF THE DRAWINGS The present invention will now be described m more detail and with reference to the accompanying drawings, in which: 20 Figure 1 shows the protein adsorbed from 50 mL of whey at pH 6.5 as a function of the substitution level of QA groups on the cellulose, Figure 2 shows the composition of non-adsorbed protein remaining m the whey (50 mL) after treatment with QA cellulose (10 mL) as a function of the 25 substitution level of QA groups on the cellulose, Figure 3 shows the protein adsorbed at pH 6.5 from cheese whey retentate diluted with water (retentate + water) as a function of the substitution level of QA groups on the cellulose, and 30 Figure 4 shows the concentration of a-lactalbumm and P-lactoglobulin in skim milk after passage through a column (10 mL) of either QA GibcoCel™ or QA cellulose with 1.84 meq/g 35 DESCRIPTION OF THE INVENTION As defined above, the present invention provides new processes useful for separating whey proteins from whey protein-containing solutions. 171402 vl WGN * 11 The applicants have now found that it is possible to prepare QA derivatives of hydroxyalkylated regenerated cellulose having substitution levels higher than those described m the prior art We have also surprisingly found that 5 such derivatives, where they exceed substitution levels of 1.4 meq/g, possess advantages over QA derivatives of hydroxylated cross-linked regenerated cellulose having lower substitution levels, in that they have a significantly higher effective protein binding capacity when used to recover protein from protein containing solutions having more than a relatively low ionic strength, 10 and in particular whey protein-containing solutions such as whey, whey protein concentrates and milk The finding that a substituted QA-denvatised anion exchanger of the ) GibcoCel™ type having a minimum substitution level of 1.4 meq/g has an 15 improved protein binding capacity over currently available QA anion exchangers (which have a level of denvatisation of up to 1.2 meq/g) for solutions such as whey is particularly surprising, m view of the fact that the corresponding SP cation exchangers having 0.8 and 1.4 meq/g have been found to be almost equally effective at adsorbing protein from whey (Ayers & 20 Peterson NZ J Dairy Sci and TechnoL, 20, 129-142, 1985). Corresponding SP cation exchangers having substitution levels greater than 1.4 meq/g would thus not be expected to show any advantages. It is these findings by the applicants which form the basis of the present 25 invention The present invention relates to processes for separating whey proteins from whey protein-containmg solutions which involve the use of the novel anion exchangers described herein These anion exchangers comprise QA 30 derivatised, hydroxy (c2-c4) alkylated and cross-linked regenerated cellulose, in which the level of denvatisation with the QA groups is 1.4 meq per dry gram of anion exchanger (meq/ g) or greater. In its broadest aspect, the present invention therefore provides a process of 35 separating a preselected whey protein or group of whey proteins from a whey protein-containing solution The process includes the step of contacting an anion exchanger as defined above with a whey protein-containmg solution, at a pH of a level at which the preselected protein or proteins will be 171402 vl WGN • 12 adsorbed (bound) to the anion exchanger. The process will then include the additional step(s) of recovering either one or both of (1) the whey protein(s) adsorbed on the anion exchanger, by desorption of the proteins, and (2) the breakthrough whey protein or proteins not adsorbed on the exchanger. 5 Although not essential, it is often desirable from an economic perspective to recover both the adsorbed protein and the breakthrough protein. The whey protein-containmg feed solution used in the process of the present invention can be any solution containing a whey protein or proteins, 10 including whole milk, skim milk, whey, ultrafiltered or microfiltered milk or whey, reconstituted WPC (whey protein concentrate) or MPC (milk protein concentrate), or any other milk derived material containing whey proteins. The step of contacting the whey protein containing solution with the anion 15 exchanger to adsorb the desired protein or proteins can be carried out in any convenient manner. Preferred methods are to carry out this step in a stirred bed of anion exchanger or in a column of the anion exchanger. It is generally preferred that the adsorption step be carried out at a temperature of less than about 20°C, more preferably at around 8-15°C, to minimise the 20 growth of mesophilic bacteria. It is also preferred that the contact time of the anion exchanger with the whey protein solution is less than about 2 hours, more preferably less than about 1 hour. Those persons skilled m the art will appreciate that by selecting an 25 appropriate pH at which a desired whey protein or group of whey proteins is known to bind to an anion exchanger, selective adsorption of the desired whey protein or group of whey proteins can be achieved. The following table shows how anion exchangers can be used to bind one or more proteins from whey to produce whey protein isolates and how other proteins are left behmd 30 m the treated whey stream The breakthrough proteins may be recovered, to give whey protein concentrates with enriched levels of those proteins (and depleted levels of protein removed) Alternatively, some or all of the breakthrough proteins may be recovered by further anion or cation exchange 35 Table 171402 vl WGN * 13 Protein* Whey protein Composition (%) Isoelectric pH (IEP) Adsorption on Anion Exchangers At pH 3-5 At pH 5-8 GMP 20 2-4 Yes Yes P-Lg 46 5 No Yes a-Lac 18 5 No Yes IR 6 «7 No No Other 10 Vanous _ *GMP - glycomacropeptide, also known as caseinomacropeptide (CMP) or casein derived peptide (CDP) Only found m sweet wheys, not acid wheys 5 P-Lg - p-lactoglobulm a-Lac - a-lactalbumm Ig - immunoglobulin Other - BSA, lactoferrm and other minor proteins. 10 The steps of recovery of either or both of the adsorbed protein and the breakthrough protein can be carried out using any suitable method known in the art, for example by elution of the adsorbed protein (which may be achieved for example using salt, dilute acid or a mixture of the two), followed by ultrafiltration and/or diafiltration if desired, and evaporation, freeze-15 drying and/ or spray drying. ^ The QA anion exchangers useful m the processes of the present invention have a level of substitution of the QA groups of 1.4 meq/g or greater. It is preferred that in the QA anion exchangers, the level of substitution of the QA 20 groups is m the range of from about 1 4 to about 2.5 meq/g, more preferably from about 1.5 to about 2.5 meq/g, and most preferably from about 1.7 meq/g to about 2.5 meq/g. In this specification, the term "QA" or "quaternary amino", when used in the 25 context of ion exchangers, means a functional group selected from a group of the formula -Ri-Z, wherein Ri is a lower alkylene group containing 1 to 3 carbon atoms and optionally substituted with a hydroxyl group, and Z is a quaternized amino group of the formula: -NR2R3R4+OH- or salts thereof, 171402 vl WGN * 14 wherein R2, R3 and R4 are each a lower alkyl group containing 1 to 4 carbon atoms, optionally substituted with a hydroxyl group, or a further group of the formula -Ri-NR2RsR4+OH- or salts thereof wherein Ri, R2, R3 and R4 are as defined above. Examples of suitable QA groups are 5 -CH2CH2N+R2R3R4 Cl~ and -CH2CHOHCH2N+R2R3R4 Cl~, wherein R2, R3 and R4 are the same or different and are selected from -CH3, -CH2CH3, -ch2ch2oh, -ch2chohch3, -CH2CH2N+R2R3R4Cr and -ch2chohch2n+r2R3R4 c r 10 The matrix for the anion exchangers used m the processes of the present invention comprises a water insoluble, hydrophilic, water swellable hydroxy(c2-c4)alkylated and cross-linked regenerated cellulose. Such matrices and processes for preparing them are described for example m US ^ Patent 4,175,183 (John S Ayers), the full contents of which are incorporated 15 herein by reference. By way of example, a suitable cellulose matrix can be prepared by reacting commercially available granular or beaded regenerated cellulose with epichlorohydnn and propylene oxide in the presence of a strong base (conveniently NaOH). Such matrices may be useful for repetitive use on a large industrial scale 20 The QA anion exchangers used having a substitution level of 1.4 meq/g or greater may be prepared by reacting a cellulose matrix as described above with a suitable alkylating agent capable of derivatising the cellulose with QA groups Conveniently, the alkylating agent may be an agent containing 25 quaternary ammonium groups, preferably (3-chloro-2- } hydroxypropyljtrimethylammonium chloride (CHPTAC) or glycidyltrimethyl ammonium chloride (GTAC), and the reaction carried out in the presence of a strong base, conveniently sodium or potassium hydroxide. CHPTAC and GTAC are known reagents for introducing QA groups into cellulose, but at 30 lower substitution levels (see, for example Carbohydrate Polymers 19, 167- 169, 1992). However, m order to prepare the anion exchangers having the level of QA substitution useful in the processes of the present invention, it will usually be necessary to employ very concentrated solutions of reagents. For example, it is preferred that when CHPTAC is used as the alkylating 35 agent, the concentration of CHPTAC reagent is about 50 wt% or greater, more preferably about 60 wt% or greater, and when GTAC is used, the concentration of GTAC reagent is greater than 50% w/v, more preferably about 70% w/v or greater 171402 vl WGN • 15 By way of example, the following process may be used to prepare the QA anion exchangers having a substitution level of 1.4 meq/g or greater. 5 A water insoluble, hydrophilic, water swellable hydroxypropylated and cross linked regenerated cellulose may be prepared by first forming a mixture of 10 g regenerated cellulose with 3-10 mL of propylene oxide, 0.5-1 mL of epichlorohydnn and 8-20 mL of aqueous sodium hydroxide solution at a concentration of 15-40% (w/v), or 10-15 mL of aqueous sodium hydroxide 10 solution at a concentration of 20-30% (w/v). The mixture is then reacted at 40- 60° C for 1-4 hours At the end of reaction, most of the sodium hydroxide remains in the matrix still, as only the reactions of epichlorohydrin consume base. It is preferable to leave the hydroxide in the cellulose for further reaction with the alkylating reagent 15 When GTAC is used as the alkylating agent, 15-20 mL of 70% (w/v) solution is mixed in with the cellulose and reaction accomplished at 10-50° C over 1-8 hours, preferably 20-25°C for 2-3 hours. The reaction of GTAC is catalysed by hydroxide The reaction time and temperature are thus not greatly dependent 20 on the amount of GTAC added The amount of GTAC reagent added will be selected to achieve the desired substitution level. When CHPTAC is used as the alkylating agent, 12-20 mL of a 60 wt % solution is mixed m with the cellulose and reaction accomplished at 20-50°C for 2-24 25 hours, preferably at 25°C for 6-24 hours, although the time can be shortened by heating to 60-80°C for 1-2 hours at the finish. Hydroxide is consumed during this reaction and the reaction time increases as the amount of reagent used is increased as a result of consumption of hydroxide by the competing alkylation and hydrolysis reactions 30 In the case of both the GTAC and CHPTAC reagents it is preferable to keep the volume added to 20 mL or less (when working with the above proportions) so that a separate aqueous phase does not separate out from the cellulose. To limit the competing hydrolysis reactions of the reagents it is desirable to limit 35 the amount of water present m the reaction mixture and use the highest concentration of reagent available 171402 vl WGN * 16 In either of the above processes, it is possible to repeat the QA derivatisation procedure, if required, where a relatively high level of derivatisation (such as around 2 0 meq/g or higher) is desired. In such cases one would use the following alternative procedure. 5 Alternatively, the QA anion exchangers useful m the processes of the present invention may be prepared by using as the starting material a commercially available QA hydroxyalkylated and cross-linked regenerated cellulose, such as that sold as QA GibcoCel™, which has a QA substitution level of 1.2 meq/g. 10 The applicants have found that a higher QA substitution level can be achieved by further processing the already denvatised exchanger using alkylating agents bearing quaternary ammonium groups such as GTAC and CHPTC, again in the ^ presence of a strong base 15 By way of example, the following process may be used to prepare the QA anion exchangers useful in the processes of the present invention, having a substitution level of 1 4 meq/g or greater, using a similarly but lower substituted exchanger (conveniently QA GibcoCel™) as a starting material. 20 QA GibcoCel™ in its hydrated form has a dry matter content of only 12-13%. Because of the large amount of water already present in the product, it is often preferable to process this further as a slurry with alkylating agent and base. Accordingly, hydrated QA GibcoCel™ is mixed with extra water and concentrated sodium hydroxide solution to form a thick slurry with a final 25 sodium hydroxide concentration of 1.5-3.0% (w/v), taking into account the ^ water already present in the hydrated QA GibcoCel™ (85-90% of its wet weight). CHPTAC, at a concentration of 60 wt%, is added m an amount of 5-25mL/ lOOg of QA GibcoCel™ to achieve the desired increase in substitution level. The conditions should be chosen such that there is an excess of hydroxide present 30 over CHPTAC in the reaction mixture. Reaction is generally accomplished at 10-50°C for 2-24 hours, preferably 20-30°C for 6-24 hours, or 17 hours with a further 1-2 hours at 60-80°C In either of the above methods of preparing a QA anion exchanger of the 35 invention, the sodium hydroxide could be replaced by an equivalent amount of potassium hydroxide. 171402 vl WGN * 17 A number of specific embodiments of the processes of the present invention of separating whey proteins from whey protein-containing solutions will now be described. In one embodiment, the process comprises the step of contacting the anion exchanger with whey, UF retentate or reconstituted WPC, at a pH of from about 5 to about 8, to selectively adsorb whey proteins other than immunoglobulins The adsorption step is followed by recovery of one or both of the adsorbed whey proteins (by elutmg these from the exchanger) and the breakthrough protein. In this embodiment of the invention, elution and recovery of the adsorbed proteins will produce a WPI. The residual protein in the breakthrough is enriched in immunoglobulins and recovery of this protein by known procedures, such as cation exchange or ultrafiltration, will produce an immunoglobulin isolate or immunoglobulin enriched WPC respectively In another embodiment of the process of the present invention, the process includes the step of contacting the anion exchanger with whey, UF retentate or reconstituted WPC at a pH of about 5 to about 8, under conditions in which P-lactoglobulm and acidic peptides and proteins (includiing GMP when the whey protein containing solution is sweet whey or derived from sweet whey) are preferentially adsorbed The desired preferential adsorption can conveniently be achieved by providing insufficient anion exchanger capacity for both a-lactalbumm and p-lactoglobulm to bind to the exchanger. Under such conditions, p-lactoglobulm will bind to the exchanger in preference to <x-lactalbumin Again, m this embodiment of the invention the adsorption step is followed by recovery of one or both of the adsorbed whey proteins and the breakthrough whey protein. Recovery of the adsorbed proteins from the exchanger will produce a WPI enriched in p-lactoglobulin and acidic peptides and proteins (including GMP if present m the whey). The partially deproteinated breakthrough may be further processed by known procedures such as anion exchange, cation exchange or ultrafiltration to recover additional protein, in particular a-lactalbumm and/or immunoglobulin. For example, concentration of the breakthrough by ultrafiltration followed by drying will produce a WPC 171402 vl WGN * 18 powder enriched in both a-lactalbumm and immunoglobulins compared with a conventional WPC. In a further embodiment of the process of the present invention, the process 5 includes the step of contacting the anion exchanger with sweet whey, a UF retentate derived from sweet whey, or reconstituted WPC from sweet whey, at a pH of about 5 or below, to selectively adsorb glycomacropeptide (GMP). It is generally preferred that the pH of the sweet whey, UF retentate or 10 reconstituted WPC is contacted with the anion exchanger at a pH of about 4 5 to about 5.0, more preferably about pH 4.7-4.9. However, a pH of 3.0-4.5 may be used when the preselected whey protein is a glycosylated subfraction of GMP 15 In this embodiment of the invention, the GMP (or a glycosylated subfraction of GMP) may then be recovered from the anion exchanger and/or the breakthrough protein recovered as a WPC depleted in GMP (or a glycosylated subfraction of GMP) 20 In a further embodiment of the process of the present invention, the process includes the step of contacting the anion exchanger with an acid whey, a UF retentate derived from an acid whey, or reconstituted WPC from an acid whey, at a pH of about 5 or below, to selectively adsorb acidic peptides and proteins substantially free of the major whey proteins. 25 It is generally preferred that the pH of the acid whey, UF retentate or reconstituted WPC is contacted with the anion exchanger at a pH of about 3 to about 5 0, more preferably about pH 4.7-4.9. 30 In this embodiment of the invention, the acidic peptides and proteins may then be recovered from the anion exchanger and/or the breakthrough protein recovered as a WPC depleted m acidic peptides and proteins. In yet a further embodiment of the process of the present invention, the 35 process includes the step of contacting the anion exchanger with whole milk, skim milk or ultrafiltered milk to adsorb whey proteins, particularly p-lactoglobulin. The milk or milk retentate is preferably contacted with the ion exchanger at a pH of from about 6-7, more preferably about pH 6.6. 171402 vl WGN * In this embodiment of the invention, the adsorbed whey proteins may be recovered from the anion exchanger as a WPI. Alternatively, or m addition, the breakthrough may be recovered as a whey protein reduced milk, skim 5 milk, retentate or milk protein concentrate, with useful properties such as enhanced heat stability The invention will now be described in more detail with reference to the following non-limiting examples 10 EXAMPLES | Example 1 QA GibcoCel™ HG2 (1 17 meq/g), an anion exchanger made from granular 15 regenerated cellulose, was obtained from Life Technologies Ltd, Auckland, New Zealand It was suspended m water and then collected on a sintered glass filter where it was washed with 1 M hydrochloric acid, water, 1 M sodium hydroxide and finally de-ionised water It was then drained of excess water by vacuum filtration. This QA cellulose in its hydroxide form was then further alkylated to 20 raise the density of positively charged QA groups. The QA GibcoCel[OH~] was made up to a thick slurry by the addition of water and 30% (w/v) aqueous sodium hydroxide. The mixture was chilled before adding (3-chloro-2-hydroxypropyl)tnmethylammonium chloride (60 wt. % 25 solution in water) The amounts used are shown in Table 1. These ingredients | were mixed as a slurry for 17 hours at room temperature followed by 2 hours at 60° C The QA cellulose products were collected on filters and washed with water, 1 M hydrochloric acid and de-ionised water before removing the excess water by vacuum filtration 30 Small samples of each product, and the starting QA GibcoCel, were analysed to determine their substitution levels of QA groups. About 5 g of the moist products were converted to their hydroxide form by washing with 1M sodium hydroxide followed by demmeralized water The samples were then titrated in 1 35 M sodium chloride with 1 00 M hydrochloric acid to an end-point of pH 4. After titration each sample was collected on a dry tared sintered-glass filter, washed with water and dried overnight at 105' C The substitution level was calculated as the small ion exchange capacity (S.I.C.) in milli-equivalents per dry gram 171402 vl WGN * 20 (meq/g), i.e. S I C. = V/wt where V = volume in mL of 1.00 M HC1, and wt = dry weight of the sample (g) Further samples in the chloride form were assayed for their protein binding 5 capacities. A 0 5% solution of P-lactoglobulin was prepared in 0.01 M sodium dihydrogen phosphate The pH of this solution was adjusted to 7.5 by the careful addition of 5 M sodium hydroxide. Aliquots (20 mL) were transferred to vials containing weighed samples of moist QA cellulose (300-400 mg). The vials were then sealed and gently mixed for 2 hours at room temperature. They were 10 left to stand for 2-5 minutes before a sample of the supernatant was taken and filtered through a 2 mL disposable column fitted with a porous frit. A 1 mL sample of the filtrate was added to 20 piL of 1 M hydrochloric acid and made up to 10 mL total volume with water before measuring the absorbance at 280 nm. The dry matter of the ion exchanger used was determined by drying samples 15 (0 5-1 0 g) in triplicate The capacity of the exchanger, grams of protein per gram of dry ion exchanger, was calculated by comparison with an Aqso reading of the original protein solution diluted similarly. The results, shown m Table 1, clearly indicate that the substitution level of 20 quaternary ammo groups on QA GibcoCel can be raised to 2 meq/g by this alkylation procedure but that there is no benefit for the p-lactoglobulin (P-lg) capacity under conditions that are typically used to measure protein capacity. In fact the capacity deteriorated, particularly for preparation #3. 25 Table 1 Preparation Details and Properties of QA Celluloses Preparation QA GibcoCel Prepn #1 Prep11 #2 Prepn #3 QA GibcoCel (wet g) 45 45 45 Water (mL) _ 31 31 28.5 30% NaOH (mL) - 5 5 7.5 CHPTAC* (mL) _ 3 6 9 Properties S I C (meq/g) 1 17 1.51 1.84 2.02 P-lg capacity (g/g) 1 85 1.75 1.65 1.38 171402 vl WGN * 21 *3-chloro-2-hydroxypropyltrimethylammonium chloride, 60 wt % solution in water Example 2 5 (a) Preparation of Hydroxypropyl Cellulose (HP-Cellulose) The same Grade 2 (150-250 mm) granular regenerated cellulose as used to make the commercially available anion exchanger QA GibcoCel used in Example 1, was obtained from Life Technologies Ltd, Auckland, New Zealand. A sample (10 g) of this was mixed m a stainless steel vessel with cold 30% (w/v) 10 aqueous sodium hydroxide (15 mL) and 0.7 mL of epichlorohydrm dissolved in 5 mL of propylene oxide The mixture was stirred thoroughly until the cellulose had finished swelling and all the liquid had been absorbed. The reaction vessel was then sealed and placed m a water bath at room temperature and heated to 50°C over 30 minutes After one hour at 50°C the reaction vessel was cooled 15 and the damp, friable cellulose powder (cross-linked and hydroxypropylated cellulose, HP-cellulose) was treated further without washing. (b) Alkylation with (3-chloro-2-hydroxypropyl)tnmethylammonium chloride (CHPTAC) 20 After chilling the reaction vessel and contents, an aqueous solution of (3- chloro-2-hydroxypropyl)tnmethylammonium chloride (18 mL of 60 wt. %) was slowly added to it while stirring thoroughly. It was then held at 25°C for 17 hours followed by 1 5 hours at 60°C The QA cellulose product was soaked in excess water and collected on a filter, washed with water, 1M hydrochloric acid 25 and then de-ionised water Samples were analysed as described m Example 1. The substitution level was found to be 2.08 meq/g, the P-lactoglobulin capacity 2.03 g/g. Example 3 30 A sample of QA cellulose (2.08 meq/g) from Example 2 was further processed m place of QA GibcoCel (1 17 meq/g) as described in Example 1 for preparation #2 Six mL of (3-chloro-2-hydroxypropyl)trimethylammonium chloride was reacted with 45 g of QA cellulose[OH~], 31 mL of water and 5 mL of 30% (w/v) aqueous sodium hydroxide The product had a substitution level of 2.52 35 meq/g, and a (3-lactoglobulin capacity of 1.96 g/g. 171402 vl WGN * 22 Example 4 Clarified cheese whey was adjusted from pH 5.7 to 6.5 with aqueous sodium hydroxide Aliquots (50 g) of this were then mixed at room temperature with 5 5 mL (3.33 g) and 10 mL (6.67 g) samples of QA GibcoCel and of the more highly substituted QA celluloses prepared from it (Preparations #1, #2 and #3 from Example 1) (All the QA celluloses after washing and draining were found to have a settled volume of 1 5 mL/wet g by separate experiment where a sample (about 10 g) was allowed to settle in water in a 25 mL measuring cylinder 10 overnight) After mixing for 1 hour, the QA celluloses were separated from the protein-depleted whey on sintered-glass filters and washed with water. The combined filtrate and washings (60 g) were analysed for total nitrogen and nonprotein nitrogen to determine the residual protein concentrations. A sample of the cheese whey at pH 6.5 was similarly analysed and the amount of protein 15 (%) adsorbed by each of the QA celluloses calculated. The results, seen in Figure 1, clearly show the increase m protein adsorption achieved as the substitution level of the QA GibcoCel was raised above 1.2 meq/g. Samples of the whey and the protein-depleted filtrates were also analysed by 20 reverse phase HPLC to determine the concentrations of the individual proteins, a-lactalbumin, p-lactoglobulm, glycomacropeptide and immunoglobulin using the method published m J Chromatography A, 878 (2000), 183-196. These values were then divided by the total protein concentration to determine the amount of each protein as a percentage of the total protein remaining m the 25 mixture The results, when 50 g of whey was mixed with 10 mL (6.67 g) of QA cellulose at pH 6 5, are shown m Figure 2 To obtain the efficient adsorption of P-lactoglobulin and glycomacropeptide, it was necessary to have a substitution 30 greater than 1.2 meq/g on the QA cellulose anion exchanger WPC powders produced from the filtrates would then be considerably more enriched in a-lactalbumin and immunoglobulins compared with WPC prepared from whey or the filtrate from QA GibcoCel as shown m Table 2. 171402 vl WGN * 23 Table 2 a-Lactalbumin and Immunoglobulin in Whey 5 before and after treatment with QA Cellulose (% of total protein present) QA Cellulose treated whey Protein Whey GibcoCel (1 17 meq/g) Prepn #1 (1 51 meq/g) Prepn #3 (2.02 meq/g) a-Lactalbumm 18 29 40 49 Immunoglobulin 6 11 16 21 10 The QA cellulose (2 08 meq/g) from Example 2, which was made m a single quaternization step, behaved similarly to the further processed QA GibcoCel preparation #3 shown here. Example 5 15 UF retentate (21% TS, 15% protein), prepared commercially from the same cheese whey as used in Example 4, was diluted with water and adjusted from pH 5 7 to 6 5 with aqueous sodium hydroxide. The amount of water used for dilution was either half or one and a half times the weight of retentate as shown in Table 3, i.e either (2+1) or (2+3) respectively. Samples, 10 g or 16.7 g 20 respectively, of these diluted retentates were then added to 9.6 mL (6.4 g) of the four QA celluloses from Example 1 and mixed for one hour. After this the QA celluloses were separated from the protem-depleted-retentate on sintered-glass filters and washed with water until the filtrate was clear. The combined filtrate and washings (30 g) were analysed for total nitrogen to determine the residual 25 protein concentration m each. The diluted retentates at pH 6.5 were similarly analysed and the amount of protein (%) adsorbed by each of the QA celluloses calculated The results, presented in Figure 3, show the increase m protein adsorption 30 efficiency with increasing QA substitution level even when the retentate is diluted with one and a half times its own weight of water to lower the ionic strength This reduction in ionic strength is used to increase the binding capacity of the anion exchangers (WO Patent 97/26797). The capacities of the 171402 vl WGN * 24 four QA cellulose used here are shown m Table 4. It is surprising to find that even under these conditions of relatively low ionic strength of 3.3 mS/cm (equivalent to about 30 mM NaCl) that the QA GibcoCel does not have sufficient density of charged groups to exhibit optimum adsorption capacity. 5 Table 3 Conditions for protein adsorption from whey and diluted retentate Diluted Retentate Whey* (2 + 1)** (2 + 3)** Protein conc , % 0.68 10.1 6 0 Weight used, g 50 10 16.7 Conductivity at pH 6 5, mS/cm 6.7 4 8 3.3 QA cellulose, mL 5 9 6 9 6 Protein load, mg/mL of QA 68 105 104 * Conditions used m Example 4 10 ** Parts of retentate plus parts of water Table 4 Protein Capacities (mg/mL) of the QA celluloses QA Gibcocel (1 17 meq/g) Prepn #1 (1 51 meq/g) Prepn #2 (1.84 meq/g) Prepn #3 (2.02 meq/g) Whey* 21 30 35 40 Retentate (2+1)** 52 58 63 67 Retentate (2+3)** 58 65 70 72 15 Results from Example 4 Parts of retentate plus parts of water 20 25 The protein (WPI) was recovered from each of the washed QA celluloses by transferring it from the funnel to a beaker and adding sufficient water to enable it to be stirred Sodium chloride (5M) was added to make the solution about 80 mM and then the pH was adjusted to 2.0 with 10 % hydrochloric acid. After stirring for a further 50 minutes the QA cellulose was returned to its filter and washed with water The combined filtrate and washings (eluate) was neutralised to pH 6-7 and made up to 50 mL with water and analysed for total nitrogen (protein) A greater yield of WPI was obtained in the eluates from each 171402 vl WGN * 25 of the QA celluloses with raised substitution levels (1.51-2.02 meq/g) than that from the QA GibcoCel (1 17 meq/g) 10 Example 6 Two 10 mL columns were packed with GibcoCel QA (1.17 meq/g) and QA cellulose (1 8 meq/g) (Preparation #2 from Example 1) respectively. Skim milk (150 g) was passed through each column over 4-5 hours followed by 10 mL of water to displace the last of the milk from each column. The first 5 mL of breakthrough (effluent) from the column was discarded and then 155 g of breakthrough and washings were collected as one bulk fraction except for 0.5 mL samples which were collected separately every 20 mL. 15 Samples of the original skim milk, the bulk fraction of treated milk and column effluent samples were each diluted 1 1 with 0.2 M acetate buffer, pH 4.0, to precipitate casein which was removed by centrifugation. Samples of the whey supernatant were analysed for BSA, a-lactalbumm and p-lactoglobulin by reverse phase HPLC The results are shown in Table 5 and Figure 4. 20 Table 5 Whey protein adsorption (%) from 150 g of skim milk by 10 mL of QA GibcoCel (1.17 meq/g) and Preparation #2 (1.84 meq/g) QA GibcoCel Preparation #2 P-lactoglobulm 62 92 a-lactalbumm 10 20 BSA 17 35 25 30 The higher substituted QA cellulose achieved > 90% removal of P-lactoglobulm from 15 column bed volumes of skim milk versus only about 60% for QA GibcoCel Figure 4 shows that QA cellulose with 1.84 meq/g can treat about twice the volume of skim milk as can QA GibcoCel before P-lactoglobulin appears m the breakthrough m significant quantities. The P-lactoglobulin and other proteins were recovered from each of the QA celluloses by transferring it from the column to a beaker and treating it as described m Example 5. 171402 vl WGN * 26 10 15 20 Example 7 A sample (200 g) of freshly prepared UF retentate (21% TS, 15% protein), commercially produced from cheese whey, was diluted with 200 mL of water and adjusted m pH to 4 7 with 10% hydrochloric acid. The resulting solution had a glycomacropeptide (GMP) concentration of 1.2% which was about 16 % of the total protein present Aliquots (28 g) of this (1 + 1) diluted retentate were added to samples of QA celluloses (equivalent to 20 or 28 mL) previously weighed into 50 mL beakers. These were stirred for 40 minutes whilst maintaining the pH at 4 7-4 8 with 1 M sodium hydroxide. Each QA cellulose was then collected on a smtered-glass filter and washed with water. The combined filtrate and washings (breakthrough) was made up to 50 g with water for analysis On a production scale the breakthrough could be neutralised and concentrated by ultrafiltration to give a WPC with minimal GMP present. Each QA cellulose was transferred to a clean dry beaker and water added to give a total weight of 30 g (QA cellulose plus water). To this was added 480 jiL of 5 M sodium chloride to make the solution approximately 80 mM. The pH was then adjusted to 2 0 with 10% hydrochloric acid to elute the GMP. After stirring for 40 minutes the QA cellulose was returned to its filter and washed with water The combined filtrate and washings (eluate) was neutralised to pH 6-7 and made up to 40 g for analysis 25 The breakthrough and eluate were analysed for GMP, a-lactalbumin, (3-lactoglobulin and BSA by HPLC using a reverse phase column. The GMP results are shown m Table 6 30 Table 6 Selective GMP Adsorption from 28 g of diluted Retentate (1+1) by QA Celluloses and Elution therefrom QA Cellulose Yield of GMP (%) Type Substn meq/g) Volume (mL) Breakthrough Eluate Preparation #2 1 84 20 14 78 QA GibcoCel 1 17 20 28 68 QA GibcoCel 1 17 28 16 75 171402 vl WGN * The more highly substituted QA cellulose (Preparation #2 from Example 1) achieved a significantly higher yield of GMP in the eluate compared with QA GibcoCel. For the latter to achieve the same yield required at least a 40% increase m the volume of ion exchanger used (28 mL in place of 20 mL). In 5 all three cases the eluate fraction (GMP) contained only 14-17% of the total protein present in the original 28 g of diluted retentate. Furthermore, a-lactalbumin, (3-lactoglobulin and BSA together made up only 6-7% of the protein in the eluate showing that the adsorption was selective for GMP. 10 INDUSTRIAL APPLICATION The applicants have found that the novel anion exchangers described herein have a higher protein capacity under all conditions of ionic strength, except low ionic strength (eg < 25 mM) than known, commercially available QA 15 denvatised hydroxyalkylated cross-linked regenerated cellulose anion exchangers with a lower level of QA substitution. The applicants have further found that the present anion exchangers maintain their protein capacity up to modest ionic strength, about 50 mM NaCl, allowing them to be more industrially useful than known commercially available QA 20 derivatised hydroxyalkylated cross-linked regenerated cellulose anion exchangers with a lower level of QA substitution. The latter loses capacity immediately the ionic strength is raised above the minimum level provided by the dilute buffer salts, i.e about 10 mM. 25 It is therefore believed that the present invention, involving the use of the anion exchangers described herein, provides processes of separating whey proteins from whey protein-containing solutions which offer increased efficiency over processes known m the art. 30 Although the invention has been described with reference to particular embodiments, those persons skilled m the art will appreciate that variations and modifications may be made without departing from the scope of the invention as defined m the accompanying claims. 171402 vl WGN * </div>

Claims

<div class="application article clearfix printTableText" id="claims"> 28 WHAT WE CLAIM IS:

1. A process of separating a preselected whey protein or group of whey proteins from a whey protein-containing solution, wherein the 5 process comprises the following steps: (a) contacting a whey protein-containing solution with an anion exchanger at a pH level at which the preselected protein or group of proteins adsorbs to the anion exchanger, and wherein the anion 10 exchanger comprises a water insoluble, hydrophilic, water swellable, hydroxy (c2-c4) alkylated and cross-linked regenerated cellulose, derivatised with quaternary ammo (QA) groups, wherein the level of substitution of the QA groups is 1.4 milli-equivalents per dry gram I of anion exchanger (meq/g) or greater; and 15 (b) recovering either or both of the following: (1) the whey protein or proteins adsorbed in step (a) and (2) the breakthrough whey protem or proteins not adsorbed m step (a). 20

2 A process as claimed m claim 1, wherein the whey protein- containmg solution is whole milk, skim milk, whey, ultrafiltered or microfiltered milk or whey, reconstituted WPC (whey protein concentrate) or MPC (milk protein concentrate), or any other milk derived material containing whey proteins. 25 ^

3 A process as claimed in claim 1, wherein, the level of substitution of QA groups on the anion exchanger is from about 1.4 to about 2.5 meq/g 30

4 A process as claimed m claim 3, wherein the level of substitution of QA groups is from about 1 5 to about 2.5 meq/g.

5 A process as claimed in claim 4, wherein the level of substitution of QA groups is from about 1 7 meq/g to about 2.5 meq/g. 35

6. A process as claimed m claim 1, wherein the cellulose is hydroxypropylated cross-linked regenerated cellulose. 171402 vl WGN * 29

7 A process as claimed in claim 6, wherein the cellulose is in granulated or beaded form.

8. A process as claimed m claim 1, wherein step (a) comprises 5 contacting the anion exchanger with a whey protein containing solution selected from whey, UF retentate and reconstituted WPC, at a pH of from about 5 to about 8, to selectively adsorb whey proteins other than immunoglobulins 10

9 A process as claimed m claim 1 or 8, wherein, the adsorbed proteins are recovered from the anion exchanger to produce a whey protein isolate (WPI) I

10. A process as claimed in claim 1 or 8, wherein the breakthrough 15 whey proteins are recovered separately, to produce an immunoglobulin isolate or an immunoglobulin enriched WPC.

11 A process as claimed in claim 10, wherein the breakthrough whey proteins are recovered by cation exchange. 20

12 A process as claimed in claim 1, wherein step (a) comprises contacting the anion exchanger with a whey protein containing solution selected from whey, UF retentate and reconstituted WPC, at a pH of from about 5 to about 8, under conditions m which p- 25 lactoglobulm and acidic peptides and proteins including, when | present, glycomacropeptide (GMP) are preferentially adsorbed to the anion exchanger

13 A process as claimed in claim 12, wherein the adsorbed proteins are 30 recovered from the anion exchanger to produce a WPI.

14 A process as claimed in claim 12, wherein the breakthrough whey proteins are recovered separately to produce an a-lactalbumin containing WPI, an a-lactalbumm/lmmunoglobulin-contaimng WPI, 35 or a WPC enriched in a-lactalbumin and immunoglobulin. 171402 vl WGN * 30

15 A process as claimed m claim 14, wherein the a-lactalbumin containing WPI is produced by further anion exchange.

16 A process as claimed m claim 14, wherein the a-5 lactalbumin/immunoglobulin containing WPI is produced by cation exchange

17. A process as claimed in claim 1, wherein step (a) comprises contacting the anion exchanger with a whey protein-containing 10 solution selected from sweet whey, UF retentate derived from sweet whey, or reconstituted WPC derived from sweet whey, at a pH of about 5 or below, to selectively adsorb glycomacropeptide (GMP). ^ 18 A process as claimed m claim 17, wherein the anion exchanger is 15 contacted with the whey protein containing solution at a pH of about 4.5 to about 5.0. 19 A process as claimed m claim 17, wherein if the preselected whey protein is a glycosylated subfraction of GMP, a pH of about 3.0 to 20 about 4 5 is used. 20 A process as claimed in any one of claims 17 to 19, wherein the adsorbed proteins are recovered from the anion exchanger, to produce a GMP-containing, or a glycosylated subfraction of GMP- 25 containing, WPI > 21 A process as claimed in any one of claims 17 to 19, wherein the breakthrough whey proteins are recovered separately, to produce a WPC depleted in GMP or a WPC depleted in glycosylated subfraction 30 of GMP 22 A process as claimed m claim 1, wherein step (a) comprises contacting the anion exchanger with a whey protein-containing solution selected from mineral acid whey, lactic acid whey, UF 35 retentate derived from acid whey, or reconstituted WPC derived from acid whey, at a pH of about 5 or below, to selectively adsorb acidic peptides and proteins 171402 vl WGN * 31 5 24 10 25 15 27 20 28 25 29 30 30 35 31 A process as claimed in claim 22, wherein the anion exchanger is contacted with the whey protein containing solution at a pH of about 3 to about 5 0 A process as claimed m claim 22 or 23 wherein, the adsorbed proteins are recovered from the anion exchanger, to produce a WPI containing acidic peptides and proteins substantially free of the major whey proteins A process as claimed in any one of claims 22 to 23, wherein the breakthrough whey proteins are recovered separately, to produce a WPC depleted in acidic peptides and proteins. A process as claimed in claim 1, wherein step (a) comprises contacting the anion exchanger with a whey protein-containmg solution selected from whole milk, skim milk or ultrafiltered milk, to adsorb proteins in the milk or milk retentate. A process as claimed m claim 26, wherein the anion exchanger is contacted with the whey protein-containmg solution at a pH of from about 6-7 A process as claimed m claim 27, wherein the anion exchanger is contacted with the whey protein-containing solution at a pH of about pH 6.6 A process according to any one of claims 26 to 28, wherein the adsorbed proteins are recovered from the anion exchanger to produce a WPI A process according to any one of claims 26 to 28, wherein the breakthrough from the anion exchanger is recovered separately, to produce a whey protein reduced milk, skim milk, retentate or milk protein concentrate A whey-protein containing product prepared by a process of any one of claims 1 to 30 MASSEY UNIVERSITY and NEW ZEALAND DAIRY BOARD By the authorised agents END OF CLAIMS £eJr PARK 171402 vl WGN * </div>