NO20051869L - Cell analysis of inducible focal adhesion kinase - Google Patents

Cell analysis of inducible focal adhesion kinase

Info

Publication number
NO20051869L
NO20051869L NO20051869A NO20051869A NO20051869L NO 20051869 L NO20051869 L NO 20051869L NO 20051869 A NO20051869 A NO 20051869A NO 20051869 A NO20051869 A NO 20051869A NO 20051869 L NO20051869 L NO 20051869L
Authority
NO
Norway
Prior art keywords
fak
cell analysis
focal adhesion
adhesion kinase
cell
Prior art date
Application number
NO20051869A
Other languages
Norwegian (no)
Other versions
NO20051869D0 (en
Inventor
Pamela Matthews Whalen
Ethan James Tekly Ung
Walter Gregory Roberts
Original Assignee
Pfizer Prod Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Prod Inc filed Critical Pfizer Prod Inc
Publication of NO20051869D0 publication Critical patent/NO20051869D0/en
Publication of NO20051869L publication Critical patent/NO20051869L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Sammendrag Den cellebaserte analysen i den foreliggende oppfinnelsen utforsker biologien til FAK i sammenheng med et induserbart genekspresjonssytem for eksogent å kontrollere FAK-ekspresjon og FAK-fosforylering ved tyrosinresidiet ved posisjon 397 (y397) . Den cellebaserte analysen i den foreliggende oppfinnelsen er fleksibel og kan måle FAKfosforylering ved Y397, total FAK-fosforylering, identifiserer mutante FAK-proteiner og måler en kombinasjon av protein og fosfotyrosin.Summary The cell-based assay of the present invention explores the biology of FAK in the context of an inducible gene expression system for exogenously controlling FAK expression and FAK phosphorylation at the tyrosine residue at position 397 (y397). The cell-based assay of the present invention is flexible and can measure FAK phosphorylation at Y397, total FAK phosphorylation, identify mutant FAK proteins, and measure a combination of protein and phosphotyrosine.

NO20051869A 2002-09-19 2005-04-18 Cell analysis of inducible focal adhesion kinase NO20051869L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41207802P 2002-09-19 2002-09-19
PCT/IB2003/003968 WO2004027018A2 (en) 2002-09-19 2003-09-08 Inducible focal adhesion kinase cell assay

Publications (2)

Publication Number Publication Date
NO20051869D0 NO20051869D0 (en) 2005-04-18
NO20051869L true NO20051869L (en) 2005-06-16

Family

ID=32030798

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20051869A NO20051869L (en) 2002-09-19 2005-04-18 Cell analysis of inducible focal adhesion kinase

Country Status (9)

Country Link
EP (1) EP1546372A4 (en)
JP (1) JP2006500022A (en)
KR (1) KR20050052663A (en)
CN (1) CN1681939A (en)
AU (1) AU2003259498A1 (en)
CA (1) CA2497434A1 (en)
NO (1) NO20051869L (en)
RU (1) RU2005107701A (en)
WO (1) WO2004027018A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100934706B1 (en) * 2006-12-07 2009-12-31 재단법인서울대학교산학협력재단 Method for Screening Anti-cancer Compounds Inhibiting Functions of TM4SF5 and Anti-cancer Composition Containing Chalcone Compounds
WO2009046129A2 (en) * 2007-10-01 2009-04-09 Columbia University Methods for treating adult respiratory distress syndrome
CN109112213B (en) * 2017-06-22 2021-01-12 中国科学院动物研究所 PCR primer for detecting focal adhesion kinase structural variant and detection method and application thereof
KR102007450B1 (en) 2018-05-31 2019-08-05 한국과학기술연구원 Screening method for new therapeuric targets of drug discovery for colon cancer and prognostic biomarkers for colon cancer screened by using the same
KR102408538B1 (en) * 2020-10-29 2022-06-15 한국과학기술연구원 Composition, kit and method for screening FAK inhibitor

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395506B1 (en) * 1991-04-18 2002-05-28 Becton, Dickinson And Company Device for monitoring cells
JP2001505779A (en) * 1996-12-11 2001-05-08 スージェン・インコーポレーテッド PYK2-related products and methods
US20010055585A1 (en) * 1997-12-03 2001-12-27 William G. Cance Frnk proteins in the treatment of tumor cells
US6133031A (en) * 1999-08-19 2000-10-17 Isis Pharmaceuticals Inc. Antisense inhibition of focal adhesion kinase expression

Also Published As

Publication number Publication date
CN1681939A (en) 2005-10-12
EP1546372A4 (en) 2006-08-23
KR20050052663A (en) 2005-06-03
EP1546372A2 (en) 2005-06-29
RU2005107701A (en) 2005-08-27
CA2497434A1 (en) 2004-04-01
NO20051869D0 (en) 2005-04-18
WO2004027018A3 (en) 2004-09-16
JP2006500022A (en) 2006-01-05
AU2003259498A1 (en) 2004-04-08
WO2004027018A2 (en) 2004-04-01

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