NL1036278C2 - A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack. - Google Patents
A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack. Download PDFInfo
- Publication number
- NL1036278C2 NL1036278C2 NL1036278A NL1036278A NL1036278C2 NL 1036278 C2 NL1036278 C2 NL 1036278C2 NL 1036278 A NL1036278 A NL 1036278A NL 1036278 A NL1036278 A NL 1036278A NL 1036278 C2 NL1036278 C2 NL 1036278C2
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- Netherlands
- Prior art keywords
- product
- plastic sleeve
- electric field
- pulsed electric
- sleeve
- Prior art date
Links
- 230000005684 electric field Effects 0.000 title claims description 25
- 238000000034 method Methods 0.000 title claims description 25
- 239000004033 plastic Substances 0.000 claims description 48
- 229920003023 plastic Polymers 0.000 claims description 48
- 239000000243 solution Substances 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 10
- 235000013305 food Nutrition 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 claims description 6
- 229920000642 polymer Polymers 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000003186 pharmaceutical solution Substances 0.000 claims description 3
- 235000013405 beer Nutrition 0.000 claims description 2
- 210000002969 egg yolk Anatomy 0.000 claims description 2
- 235000013345 egg yolk Nutrition 0.000 claims description 2
- 235000013601 eggs Nutrition 0.000 claims description 2
- 239000003889 eye drop Substances 0.000 claims description 2
- 229940012356 eye drops Drugs 0.000 claims description 2
- 235000015203 fruit juice Nutrition 0.000 claims description 2
- 229940100662 nasal drops Drugs 0.000 claims description 2
- 235000015192 vegetable juice Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 238000002474 experimental method Methods 0.000 description 9
- 240000006024 Lactobacillus plantarum Species 0.000 description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 7
- 230000002779 inactivation Effects 0.000 description 7
- 229940072205 lactobacillus plantarum Drugs 0.000 description 7
- 239000000463 material Substances 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 229920001940 conductive polymer Polymers 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000009928 pasteurization Methods 0.000 description 3
- 229920006254 polymer film Polymers 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 239000004697 Polyetherimide Substances 0.000 description 2
- 239000006229 carbon black Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000005038 ethylene vinyl acetate Substances 0.000 description 2
- 239000012212 insulator Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920001601 polyetherimide Polymers 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000010200 validation analysis Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 229920004738 ULTEM® Polymers 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000015197 apple juice Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 235000021056 liquid food Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- -1 nickel Chemical class 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 235000015205 orange juice Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/60—Preservation of foods or foodstuffs, in general by treatment with electric currents without heating effect
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/03—Electric current
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Packages (AREA)
Description
A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack
The present invention relates to a method for treatment of a 5 pumpable product using a pulsed electric field to make membranes of cells that may be present permeable.
It is known in the art to treat a pumpable product, such as a liquid food product, with a pulsed electrical field to make membranes of cells that may be present permeable. Under proper conditions, a 10 non-thermal pasteurization is achieved. This is in particular interesting in cases where the use of conservatives is not desired, heat-treatment could adversely affect the product being treated, to mention a few.
A problem of the pulsed electric field technique is that elec-15 trode material, in particular heavy metal ions such as nickel, may end up in the product treated. Another problem is that the electrodes used for pulsed electrical field method corrode over time and need to be replaced. This may lead to microbiological contamination of the product.
20 The object of the present invention is to alleviate these prob lems .
To this end, the method according to the preamble is characterized in that wherein the product is introduced in a plastic conductive sleeve and the product in the plastic sleeve is subjected to the 25 pulsed electric field using electrodes outside the plastic sleeve.
Because the electrode is not in direct contact with the product to be treated, a series of advantages is achieved, where the particular advantage(s) may depend on the product being treated. Firstly, the plastic material is a physical barrier that will prevent material 30 from the electrodes to end up in the product treated. Secondly, the electrodes can be replaced without contaminating the product. Thirdly, if the product contains compounds, such as proteins or fat having a tendency to accumulate on the electrodes, the need for maintenance of the electrodes (in particular cleaning) is reduced and the 35 reliability of the treatment is enhanced.
While non-thermal pasteurization of food products is an important field of use for the method, the term treatment is not limited to non-thermal pasteurization. Other areas of use could be cracking 1036278 2 cells, for example cells containing pharmaceutical compounds, such as proteins made using genetic engineering.
According to a preferred embodiment, the plastic sleeve is a heat sealable plastic sleeve.
5 This makes it easy to produce unitary packages containing the product.
According to a first embodiment, the product is introduced in the sealable plastic sleeve, the sleeve is heat sealed to yield a unitary package, after which the product in the unitary package is 10 treated using the pulsed electric field.
According to an alternative embodiment, the product is introduced in the sealable plastic sleeve, the product in the plastic sleeve is treated using the pulsed electric field, after which the sleeve is heat sealed to yield a unitary package containing the 15 treated product.
To optimize the efficiency of the pulsed electric field method, it is preferred for a product having a specific conductivity of X Siemens/meter and, the plastic sleeve has a specific conductivity in the range of 0,1 * X to 10 * X, preferably in the range of 0,3 * X to 20 3 * X, more preferably 0,5 * X to 2 * X.
The closer the match of the specific conductivities, the better the result, or fewer pulses and/or weaker pulses suffice. It goes without saying that for anisotropic sleeves, the relevant specific conductivity is the specific conductivity perpendicular to the sur-25 face of the plastic sleeves. For measurement of the specific conductivity reference is made to NEN-EN-IEC 61340-2-3, (Jan. 2001).
According to a favourable embodiment, for a product having a specific conductivity of X Siemens/meter, the outside of the plastic sleeve is contacted with a conducting solution having a specific con-30 ductivity in the range of 0,1 * X to 10 * X, preferably 0,3 * X to 3 * X, more preferably 0,5 * X to 2 * X, after which the product in the plastic sleeve is subjected to pulsed electric field through the conducting solution.
This helps to ensure proper contact between the electrodes and 35 the plastic sleeve, making the shape of the plastic sleeve of less significant. It is in particular attractive for treatment of unitary packages (which at the time of treatment may still be connected) containing the product to be treated. In such a case, it is preferred if 3 the unitary package (or string of unitary packages) is immersed in the conducting solution. Alternatively, instead of the conductive solution solid intermediate bodies may be used having a specific conductivity in the range of 0,1 * X to 10 * X, preferably 0,3 * X to 3 5 * X, more preferably 0,5 * X to 2 * X, after which the product in the plastic sleeve is subjected to pulsed electric field through the intermediate bodies. The intermediate bodies are shaped to fit against a plastic sleeve containing the product to be treated to achieve the desired electrical field in the product. This is in particular suit-10 able for rigid plastic sleeves.
For some applications, the specific conductivity of the product to be treated may vary, for example because the product is a product derived from nature. In such a case, it is preferred that the specific conductivity of the product is adjusted before being introduced 15 into the plastic sleeve.
Adjustment may be done by mixing the product with another batch of the product to bring it closer to the specific conductivity of the plastic sleeve. In addition or alternatively, the specific conductivity of the plastic sleeve may be chosen such that it is at the high 20 specific conductivity end of the natural variability of the product to be treated, and a concentrated ionic solution may be mixed with the product before it is treated to bring the specific conductivity of the product comprising the added ions close to the specific conductivity of the plastic sleeve. A suitable concentrated ionic solu-25 tion is for example a salt-solution or a buffer, such as citric acid-buffer .
As indicated above, an important area of use is in the food industry. Thus, according to a preferred embodiment, the product is a food product.
30 Specifically preferred food products are chosen from a liquid dairy product, fruit juice, vegetable juice, beer, raw egg, raw egg white, and raw egg yolk.
Other important areas of use are the pharmaceutical and biotechnological fields. Especially preferred products are chosen from a 35 pharmaceutical solution, eye drops or nose drops. The pharmaceutical solution is for example a protein-containing solution, e.g. one containing a protein hormone, an anti-serum etc.
4
While it is possible to use a plastic sleeve made of or containing conductive polymers, these conductive polymers tend to be relatively expensive. Hence, according to a preferred embodiment, the plastic sleeve is a polymer comprising conducting particles. This 5 makes it also easy to adapt the specific conductivity of the polymer by varying the concentration of the conducting particles.
Preferably the conducting particles are carbon-particles.
Such particles are inert, making them appropriate for many applications .
10 The invention also relates to a bag-in-a-pack comprising a bag containing a product treated obtained using the method according to the invention.
Bag-in-a-pack is a concept that has become more and more popular, in particular for food products. The present invention is par-15 ticularly suitable for increasing the shelf-life of such products.
The present invention will now be illustrated with reference to the drawing where fig. 1 depicts the treatment of a bag comprising a product using the method according to the invention, and 20 fig. 2 shows the manufacture of a sleeve, the sleeve being filled with product to be treated.
Fig. 1 shows a plastic bag 101 filled with a liquid 102 to be non-thermally pasteurized. On opposite sides of the plastic bag 101 there are electrodes 103, 104, connected to a source 105 for provid-25 ing high-voltage pulses. Suitable voltages, duration, pulse rise time and frequency depend on the product to be treated, and it is within the skill of the ordinary person to determine appropriate values. See [1]. Specs for a suitable pulsed electric field (PEF) source are:
Voltage: 10 kV/200A.
30 Rise time: 200 ns.
Pulse width: 500 - 10,000 ns.
Repetition frequency: 1 - 1000 Hz.
The plastic bag 101 was made of ethylene vinylacetate copolymer containing carbon particles. The carbon particles are in contact with 35 each other, as a result of which the plastic bag 101 is conductive. The plastic itself is known in the art and used for packaging electronic components to protect them from static charges. A suitable plastic is Carbostat, available from Warmbier (Hilzingen, Germany).
5
This plastic is heat-sealable and has a specific conductivity of 0.5 Siemens/m.
The bag 101 is moved with respect to the electrodes 103, 104 to ensure that the full content of the bag 101 is adequately subjected 5 to pulsed electrical field. The non-rigid nature of the bag 101 allows the bag 101 to accommodate for use of a reduced voltage with electrodes 103, 104 being relatively close.
It is advantageous to perform the treatment with the bag 101 submerged in a saline solution (not shown) with a specific conductiv-10 ity that is the same as the specific conductivity of the polymer. This ensures that the sides of the bag are in electrical contact when they pass through between the electrodes.
Fig. 2 discloses a method where a sleeve 201 is formed from a roll 210 of Carbostat by heat-sealing, forming a longitudinal seal 15 231. Product (arrow) is introduced via a conduit 212 into the sleeve 2C1. The product then passes two annular electrodes 203, 204 connected to a PEF source 205. After having passed the annular electrodes 203, 204 (which in reality are in contact with the plastic sleeve 201) a pouch 250 is formed by providing thermal seals 232, 20 233. Pouches 250 can be separated from the sleeve at that stage or later. The pouches 250 can be inserted into a carton to form a bag-in-a-pack.
It goes without saying that for the best results, entrapment of air inside the plastic sleeve should be avoided. Generally, the prod-25 uct to be treated will have a specific conductivity of less than 2 Siemens/m.
Experimental section
In this experiment, a Carbon Black-loaded conductive polymer 30 film (Carbostat 08/2005, Warmbier (Hilzingen, Germany)) has been used to inactivate Lactobacillus plantarum LA-10-11. The achieved results have been compared with the inactivation kinetics without polymer film (i.e. with stainless steel electrodes) and with results from literature .
35 2. System description 2.1. Treatment chamber 6 A treatment chamber with a plate-to-plate geometry has been used. The treatment chamber comprised two stainless steel (AISI-316) electrodes, separated by a ULTEM® polyetherimide (PEI) insulator.
5 Both electrodes were covered with Carbostat 08/2005 polymer (specific conductivity 0.5 Siemens/m). The circular plate electrodes (diameter of 12 mm) , which are inserted in the insulator, create a treatment zone with a volume of approximately 283 μΐ at 2.5 mm electrode distance. At the maximum pulse voltage of 10 kV, the average electric 10 field strength in a homogeneous medium equals 4 kv-mm1.
2.2. Electrical set-up
For PEF treatment in such a so-called batch chamber with an en-15 closed (i.e. fixed) volume, the applied energy needs to be limited to avoid excessive temperatures. Limitation has been reached by generating a pre-defined number of pulses of a certain voltage and pulse duration. The high-voltage pulse generator generates fully adjustable bursts of 1-100 mono-polar square wave pulses, from 2-10 kV, between 20 1-9 με pulse length (tp). A typical burst of 2x2 pulses was used dur ing these experiments. Within the burst the pulse repetition time can be varied from 1-999 ms (Tb). Repetition of more burst is done externally (Tr). The pulse generator, which is described in [2], has a bipolar stacked IGBT topology, where only the positive part has been 25 used for the experiments described here.
Electrical measurements have been carried out with a digital oscilloscope from Yokogawa (model DL-1740) . The voltage across and the current through the treatment chamber have been measured with a high voltage probe from Tektronix (model P-6015A) and a current.
30 3. Experiments
The main goal was to investigate whether it is possible to generate a high enough electric field to inactivate bacteria in the 35 newly developed treatment chamber, which is equipped with plastic composite electrodes.
All PEF experiments have been carried out in a phosphate buffer, which is prepared by mixing 2.5 ml of a 0.5 M Na2HP04.2H20 7 solution, 2.5 ml of a 0.5 M NaH2P04.2H20 solution and 600 ml of dema-terialized water. The pH of this buffer medium was adjusted at pH 4.5 by using 1 N H2SO4. The conductivity of the PEF-medium equals 0.1 S/m and was filter sterilized.
5 Validation was done microbiologically; determining the effect of the PEF inactivation treatment of Lactobacillus plants rum.
Each sample (0.1 ml) was added to 9.9 ml Peptone Physiological Saline (PPS) solution. The sample dilutions were cooled down and stored at 4 °C. The same procedure was followed for untreated control 10 samples. The next day decimal dilution series on Petri dishes filled with MRS Agar were made and incubated under anaerobic conditions during 3 days at 30 °C. Colony counts were performed using a colony counter. The concentrations of viable cells after PEF treatment (Ntreated) as well as at inoculum level (No) are expressed in Colony 15 Forming Units (CFU) per milliliter. The initial CFU (i.e. N0) of L. plantarum were 3.12*101 2 3 4 5. The mentioned reduction is calculated via r =-logi0 (Ntreated/N0) .
During the microbiological experiments the energy was kept as low as possible, typically 5-20 J.rnl"1, to avoid thermal inactiva-20 tion. Finally the data were been compared with microbial measurements in the developed treatment chamber without film covering, and with two inactivation models for Lactobacillus plantarum from literature.
Results (obtained inactivation) 2 25 3
For microbial validation of the treatment chamber eight slightly different PEF experiments with Lactobacillus plantarum were carried out. Eight experiments were done with polymer film electrodes. The results of these experiments are summarized in Table 1. 30 The number of pulses, shown in the second column were supplied as bursts of two. The pulse duration of each individual pulse, tp, was 3 μΞ or 5 με. The fourth column shows the total treatment time, tt, 4 (i.e. nxfp), which is used in the model of Gomez et al. and is discussed in the next paragraph. In the fifth and sixth column, the ap-35 plied electric field range and the dissipated energy are given. The measured logarithmic reduction is depicted together with the calcu 5 lated reduction in the last column. These calculations were done with 8 mathematical models developed by Gomez et al. [4] and Abram et al.
[3] .
Table 1: Obtained electrical and microbial data for the polymer film 5 electrode compared with the models from Gomez et al. [4] and Abram et al. [3]
Source n jp Tt Eb Qb Reduction Μ [με] [μβ] [kV.mm”1] [J.ml“l] [logio]
This ex- 4-6 3-5 . 2.2-2.8 7-17 0.8-2.1 periment ___
Gomez - - 12-30 2.2-2.8 - 0.7-2.5
Abram [4-6 [3-5 [- 2.2-2.8 |- 1.4-3.1 ~
In relation with the applied energy, a significant reduction is 10 obtained, demonstrating the suitability of inactivation of Lactobacillus plantarum by PEF with a conducting plastic as the electrode, in this case a polymer loaded with Carbon Black.
5. Conclusions 15
The developed treatment chamber, which can be equipped with any plastic film material, is successfully tested with a conductive composite Ethylene Vinyl Acetate (EVA) copolymer film. It has been proved that it is possible to inactivate Lactobacillus plantarum with 20 pulsed electric fields, where both treatment chamber electrodes are covered with the composite conductive plastic film, Carbostat. The achieved results with the covered electrodes have been compared with conventional stainless steel electrodes and with models from literature. The obtained results are all consistent with each other. The 25 maximum achieved (log10) reduction with the film electrodes is 0.8-2.1 logio with a specific energy of 7-17 J-ml-1.
6. References 30 [1]: Lelieveld, H.L.M., Notermans, S., and De Haan S.W.H., Food
Preservation by Pulsed Electric Fields, From research to application, CRC Press, ISBN 978-1-4200-4395-2.
[2]: Prins, H.A., Beurskens, R.H.S.H., Creyghton, Y.L.M., De- treux, N., De Haan S.W.H. and Roodenburg, B., 9 [3]: Abram, F., Smelt, J.P.P.M., Bos, R., and Wouters, P.C.,
Modelling and optimization of inactivation of Lactobacillus plantarum by pulsed electric field treatment, Journal of Applied Microbiology 2003, 94: 571-579 5 [4]: Gomez, N., Garcia, D., Alvarez, I., Raso, J., and Condon, S., A model describing the kinetics of inactivation of Lacto bacillus plantarum in a buffer system of different pH and in orange and apple juice, Journal of Food Engineering, 70, 2005, 7-14 10 1036278
Claims (13)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL1036278A NL1036278C2 (en) | 2008-12-03 | 2008-12-04 | A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack. |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL1036269 | 2008-12-03 | ||
| NL1036269 | 2008-12-03 | ||
| NL1036278 | 2008-12-04 | ||
| NL1036278A NL1036278C2 (en) | 2008-12-03 | 2008-12-04 | A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack. |
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| Publication Number | Publication Date |
|---|---|
| NL1036278C2 true NL1036278C2 (en) | 2010-06-07 |
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|---|---|---|---|
| NL1036278A NL1036278C2 (en) | 2008-12-03 | 2008-12-04 | A method for treatment of a pumpable product using a pulsed electric field, and a bag-in-a-pack. |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4838154A (en) * | 1985-05-31 | 1989-06-13 | Maxwell Laboratories, Inc. | Apparatus for extending the shelf life of fluid food products |
| US5690978A (en) * | 1996-09-30 | 1997-11-25 | Ohio State University | High voltage pulsed electric field treatment chambers for the preservation of liquid food products |
| WO1999063843A1 (en) * | 1998-06-05 | 1999-12-16 | Nederlands Instituut Voor Zuivelonderzoek | Pulsed electric field treatment system |
| JP2000139432A (en) * | 1998-11-05 | 2000-05-23 | Nissin Electric Co Ltd | Sterilization of liquid material and sterilizing apparatus |
| US6083544A (en) * | 1998-06-19 | 2000-07-04 | Karen M. Addeo | Process for the use of pulsed electric fields coupled with rotational retorting in processing meals ready to eat (MRE) |
| WO2000056179A1 (en) * | 1999-03-24 | 2000-09-28 | Ato B.V. | Method for treating products by high voltage pulses |
| WO2003056941A1 (en) * | 2001-12-19 | 2003-07-17 | Saligus Ab | Arrangement and method for treatment of a pumpable substance |
| WO2004041730A1 (en) * | 2002-11-04 | 2004-05-21 | Steris Inc. | Pulsed electric field system for treatment of a fluid medium |
| WO2008144499A1 (en) * | 2007-05-16 | 2008-11-27 | Old Dominion Univesity Research Foundation | System and methods for pasteurizing food using ultrashort electrical pulses |
-
2008
- 2008-12-04 NL NL1036278A patent/NL1036278C2/en not_active IP Right Cessation
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4838154A (en) * | 1985-05-31 | 1989-06-13 | Maxwell Laboratories, Inc. | Apparatus for extending the shelf life of fluid food products |
| US5690978A (en) * | 1996-09-30 | 1997-11-25 | Ohio State University | High voltage pulsed electric field treatment chambers for the preservation of liquid food products |
| WO1999063843A1 (en) * | 1998-06-05 | 1999-12-16 | Nederlands Instituut Voor Zuivelonderzoek | Pulsed electric field treatment system |
| US6083544A (en) * | 1998-06-19 | 2000-07-04 | Karen M. Addeo | Process for the use of pulsed electric fields coupled with rotational retorting in processing meals ready to eat (MRE) |
| JP2000139432A (en) * | 1998-11-05 | 2000-05-23 | Nissin Electric Co Ltd | Sterilization of liquid material and sterilizing apparatus |
| WO2000056179A1 (en) * | 1999-03-24 | 2000-09-28 | Ato B.V. | Method for treating products by high voltage pulses |
| WO2003056941A1 (en) * | 2001-12-19 | 2003-07-17 | Saligus Ab | Arrangement and method for treatment of a pumpable substance |
| WO2004041730A1 (en) * | 2002-11-04 | 2004-05-21 | Steris Inc. | Pulsed electric field system for treatment of a fluid medium |
| WO2008144499A1 (en) * | 2007-05-16 | 2008-11-27 | Old Dominion Univesity Research Foundation | System and methods for pasteurizing food using ultrashort electrical pulses |
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