MY197491A - Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof - Google Patents

Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof

Info

Publication number
MY197491A
MY197491A MYPI2019001044A MYPI2019001044A MY197491A MY 197491 A MY197491 A MY 197491A MY PI2019001044 A MYPI2019001044 A MY PI2019001044A MY PI2019001044 A MYPI2019001044 A MY PI2019001044A MY 197491 A MY197491 A MY 197491A
Authority
MY
Malaysia
Prior art keywords
methods
channelopsin
mutations
variants
identification
Prior art date
Application number
MYPI2019001044A
Inventor
Zhuo-Hua Pan
Original Assignee
Univ Wayne State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Wayne State filed Critical Univ Wayne State
Publication of MY197491A publication Critical patent/MY197491A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00Drugs for disorders of the senses
    • A61P27/02Ophthalmic agents
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0621Eye cells, e.g. cornea, iris pigmented cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Ophthalmology & Optometry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides compositions and kits including at least one nucleic acid or polypeptide molecule encoding for a mutant CoChop protein. Methods of the invention include administering a composition comprising a mutant CoChop to a subject to preserve, improve, or restore phototransduction. Preferably, the compositions and methods of the invention are provided to a subject having impaired vision, thereby restoring vision to normal levels. (Figure 1)
MYPI2019001044A 2016-08-29 2017-08-29 Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof MY197491A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662380871P 2016-08-29 2016-08-29
PCT/US2017/049158 WO2018044912A1 (en) 2016-08-29 2017-08-29 Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof

Publications (1)

Publication Number Publication Date
MY197491A true MY197491A (en) 2023-06-19

Family

ID=59859616

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2019001044A MY197491A (en) 2016-08-29 2017-08-29 Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof

Country Status (15)

Country Link
US (3) US11041004B2 (en)
EP (1) EP3504227A1 (en)
JP (1) JP7116884B2 (en)
KR (3) KR20250159068A (en)
CN (2) CN110023327B (en)
AU (4) AU2017319306B2 (en)
BR (1) BR112019003950A2 (en)
CA (1) CA3034887A1 (en)
IL (2) IL310142A (en)
MX (1) MX2019002321A (en)
MY (1) MY197491A (en)
NZ (1) NZ751983A (en)
RU (1) RU2019109021A (en)
SG (2) SG10202102061SA (en)
WO (1) WO2018044912A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY197491A (en) 2016-08-29 2023-06-19 Univ Wayne State Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof
CN113173984B (en) 2021-04-12 2022-08-30 中眸医疗科技(武汉)有限公司 Application of novel photosensitive channel protein VR1.0 in preparation of medicine for treating retinal photoreceptor cell degenerative disease
CN117858894B (en) * 2021-12-20 2025-11-25 健达九州(北京)生物科技有限公司 Optogenetic visual restoration using photosensitive GQ-coupled neuroopsin (opsin 5)
WO2024173742A1 (en) 2023-02-17 2024-08-22 Ray Therapeutics, Inc. Gene therapy compositions and methods for treating diseases of the retina
WO2025137649A1 (en) * 2023-12-22 2025-06-26 Wayne State University Method of enhancing viral mediated gene delivery

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
WO1992008796A1 (en) 1990-11-13 1992-05-29 Immunex Corporation Bifunctional selectable fusion genes
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
CA2183667A1 (en) 1994-02-22 1995-08-24 Wayne A. Marasco Nucleic acid delivery system, method of synthesis and uses thereof
EP1919497B1 (en) * 2005-07-22 2020-02-12 The Board of Trustees of the Leland Stanford Junior University Light-activated cation channel and uses thereof
JP2009536219A (en) 2006-05-04 2009-10-08 ウェイン・ステート・ユニバーシティー Recovery of visual response by in vivo delivery of rhodopsin diffusion
CN101313366A (en) 2006-06-27 2008-11-26 株式会社爱德万测试 Semiconductor test device and test method of semiconductor memory
WO2011140279A1 (en) 2010-05-04 2011-11-10 Wayne State University Aav-mediated subcellular targeting of heterologous rhodopsins in retinal ganglion cells
ES2545992T3 (en) 2010-09-08 2015-09-17 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Mutant Canalrodopsin 2
EP2699270B1 (en) 2011-04-22 2017-06-21 The Regents of The University of California Adeno-associated virus virions with variant capsid and methods of use thereof
KR102084803B1 (en) 2011-11-12 2020-03-05 메사추세츠 인스티튜트 오브 테크놀로지 Channelrhodopsins for optical control of cells
JP6395611B2 (en) * 2012-03-05 2018-09-26 ウェイン・ステート・ユニバーシティー Identification and use of channel rhodopsin-2 (Chop2) mutations
EP2968476B1 (en) * 2013-03-14 2021-05-05 Wayne State University A plasmin for use in enhancing delivery of therapeutic compounds to the eyes or for improving vision
JP2017506889A (en) 2014-02-07 2017-03-16 マサチューセッツ インスティテュート オブ テクノロジー Blue light activated ion channel molecules and uses thereof
CA2942324C (en) 2014-03-11 2024-05-14 Wayne State University A modified mglur6 promoter and methods of use
US10590181B2 (en) 2014-04-18 2020-03-17 Massachusetts Institute Of Technology Mutant channelrhodopsins with altered ion selectivity
US20160346359A1 (en) 2015-05-01 2016-12-01 Spark Therapeutics, Inc. Adeno-associated Virus-Mediated CRISPR-Cas9 Treatment of Ocular Disease
RU2754467C2 (en) 2016-03-31 2021-09-02 Спарк Терапьютикс, Инк. COMPLETELY SCALABLE METHOD FOR PRODUCING rAAV BASED ON COLUMNS
WO2017210664A1 (en) 2016-06-03 2017-12-07 Massachusetts Institute Of Technology Somatic opsins for single cell resolution optogenetics
CA3029833A1 (en) 2016-07-29 2018-02-01 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
MY197491A (en) 2016-08-29 2023-06-19 Univ Wayne State Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof

Also Published As

Publication number Publication date
KR102609571B9 (en) 2025-11-20
KR102877327B1 (en) 2025-10-27
RU2019109021A3 (en) 2021-02-02
KR20230169427A (en) 2023-12-15
AU2022201291A1 (en) 2022-03-17
SG11201901697QA (en) 2019-03-28
CA3034887A1 (en) 2018-03-08
AU2025248765A1 (en) 2025-10-30
NZ751983A (en) 2025-11-28
SG10202102061SA (en) 2021-04-29
US11041004B2 (en) 2021-06-22
IL265010A (en) 2019-04-30
AU2024200016B2 (en) 2025-07-17
IL310142A (en) 2024-03-01
RU2019109021A (en) 2020-09-29
KR20250159068A (en) 2025-11-07
WO2018044912A1 (en) 2018-03-08
AU2017319306A1 (en) 2019-04-11
US12234267B2 (en) 2025-02-25
KR102609571B1 (en) 2023-12-01
CN110023327B (en) 2024-02-27
IL265010B2 (en) 2024-06-01
CN119979612A (en) 2025-05-13
BR112019003950A2 (en) 2019-06-25
US20250163111A1 (en) 2025-05-22
US20220089660A1 (en) 2022-03-24
AU2017319306B2 (en) 2021-12-02
CN110023327A (en) 2019-07-16
KR20190075906A (en) 2019-07-01
AU2022201291B2 (en) 2023-10-05
MX2019002321A (en) 2019-10-21
US20190241628A1 (en) 2019-08-08
AU2024200016A1 (en) 2024-01-25
AU2024200016B9 (en) 2025-08-07
IL265010B1 (en) 2024-02-01
JP7116884B2 (en) 2022-08-12
JP2019528781A (en) 2019-10-17
EP3504227A1 (en) 2019-07-03

Similar Documents

Publication Publication Date Title
MX2014010664A (en) Identification of channelrhodopsin-2 (chop2) mutations and methods of use.
MY197491A (en) Identification of mutations in channelopsin variants having improved light sensitivity and methods of use thereof
AR127361A2 (en) PHARMACEUTICAL COMPOSITION OF LIPOPLEX AND RNA PARTICLES, AQUEOUS COMPOSITION AND PREPARATION METHOD
MY191030A (en) Gdf15 fusion proteins and uses thereof
ZA202501799B (en) Recombinant nucleic acids encoding cosmetic protein(s) for aesthetic applications
MX2022007122A (en) Designed ankyrin repeat domains with altered surface residues.
WO2017191274A3 (en) Rna encoding a therapeutic protein
PH12020551019A1 (en) IL-22 Fc FUSION PROTEINS AND METHODS OF USE
EA201691111A1 (en) CHEMERAL PROTEINS OF FACTOR VIII AND THEIR APPLICATION
PE20170771A1 (en) COMPOSITIONS AND METHODS OF USE TO TREAT METABOLIC DISORDERS
BR112019011860A2 (en) insulin-fc fusion protein, recombinant nucleic acid sequence encoding the same, vector, modified eukaryotic cell, kit and method for treating or preventing autoimmune diabetes
TNSN08064A1 (en) Albumin fusion proteins
MX2023001877A (en) Engineered meganucleases specific for recognition sequences in the pcsk9 gene.
PH12013502234A1 (en) Amino acid sequences directed against il-17a, il-17f and/or il17-a/f and polypeptides comprising the same
EP3939606A3 (en) Improved methods and compounds for eliminating immune responses to therapeutic agents
MX2017010880A (en) Fusarium toxin-cleaving polypeptide variants, additives containing same, use of same, and method for splitting fusarium toxins.
MX2017014730A (en) Muteins of human lipocalin 2 with affinity for glypican-3 (gpc3).
WO2020150290A3 (en) Methods and compositions for restoring stmn2 levels
MX386295B (en) MUTANT CD200 AND ITS USES
PE20161406A1 (en) TATK-CDKL5 FUSION PROTEINS, COMPOSITIONS, FORMULATIONS AND USE OF THEM
PE20210918A1 (en) COMPOSITIONS AND METHODS FOR THE TREATMENT OF MACULAR DYSTROPHY
FR3001729B1 (en) FACTOR X MUTANTS
WO2018200814A3 (en) Modified cyclic peptides and therapeutic use thereof
WO2021113440A3 (en) Oligopeptide, testing kit thereof, medical composition thereof and use of medical composition
AU2017248103A1 (en) Tobacco leaf extract and use thereof for the treatment of tobacco addiction