MY188404A - Stem cell culturing method - Google Patents

Stem cell culturing method

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Publication number
MY188404A
MY188404A MYPI2018700248A MYPI2018700248A MY188404A MY 188404 A MY188404 A MY 188404A MY PI2018700248 A MYPI2018700248 A MY PI2018700248A MY PI2018700248 A MYPI2018700248 A MY PI2018700248A MY 188404 A MY188404 A MY 188404A
Authority
MY
Malaysia
Prior art keywords
stem cells
culturing vessel
extracted
surface area
stem cell
Prior art date
Application number
MYPI2018700248A
Inventor
Alexei GLADKOV
Original Assignee
Kintarocellspower Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2016218144A external-priority patent/JP6153653B2/en
Application filed by Kintarocellspower Co Ltd filed Critical Kintarocellspower Co Ltd
Publication of MY188404A publication Critical patent/MY188404A/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A stem cell culturing method in which a second bone marrow aspirate 23 that is an intermediate layer is extracted from a first bone marrow aspirate 19 that is separated into layers; the second bone marrow aspirate 23 is cultured along with a culture medium and first stem cells are fixed on a bottom surface of a first culturing vessel; when a total surface area of the first stem cells reaches a first target ratio with respect to the bottom surface area of the first culturing vessel, the first stem cells are extracted from the first culturing vessel; top layer second stem cells are extracted from the first stem cells that are separated into layers and the second stem cells are cultured along with a culture medium, and the second stem cells are fixed on a bottom surface of a second culturing vessel; and, when a total surface area of the second stem cells reaches a second target ratio with respect to the bottom surface area of the second culturing vessel, the second stem cells are extracted from the second culturing vessel. (Figure 4)
MYPI2018700248A 2015-12-07 2016-12-06 Stem cell culturing method MY188404A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015238349 2015-12-07
JP2016218144A JP6153653B2 (en) 2015-12-07 2016-11-08 Stem cell culture method
PCT/JP2016/086206 WO2017099067A1 (en) 2015-12-07 2016-12-06 Stem cell culturing method

Publications (1)

Publication Number Publication Date
MY188404A true MY188404A (en) 2021-12-07

Family

ID=59014159

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2018700248A MY188404A (en) 2015-12-07 2016-12-06 Stem cell culturing method

Country Status (2)

Country Link
MY (1) MY188404A (en)
WO (1) WO2017099067A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4542409B2 (en) * 2004-10-14 2010-09-15 オリンパス株式会社 Method for culturing bone marrow-derived mesenchymal stem cells
CN102791276B (en) * 2010-03-10 2015-03-04 智再如股份有限公司 Cell preparation containing mesenchymal stem cells, and method for producing same
WO2015021189A1 (en) * 2013-08-06 2015-02-12 Regenerative Sciences, Llc Bone marrow adipose portion isolation device and methods
JP2015084686A (en) * 2013-10-29 2015-05-07 オリンパス株式会社 Jig for cell culture, and automatic culture medium exchange system
JP6377929B2 (en) 2014-03-27 2018-08-22 株式会社吉田製作所 Stem cell culture apparatus and stem cell culture method

Also Published As

Publication number Publication date
WO2017099067A1 (en) 2017-06-15

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