MY188404A - Stem cell culturing method - Google Patents
Stem cell culturing methodInfo
- Publication number
- MY188404A MY188404A MYPI2018700248A MYPI2018700248A MY188404A MY 188404 A MY188404 A MY 188404A MY PI2018700248 A MYPI2018700248 A MY PI2018700248A MY PI2018700248 A MYPI2018700248 A MY PI2018700248A MY 188404 A MY188404 A MY 188404A
- Authority
- MY
- Malaysia
- Prior art keywords
- stem cells
- culturing vessel
- extracted
- surface area
- stem cell
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A stem cell culturing method in which a second bone marrow aspirate 23 that is an intermediate layer is extracted from a first bone marrow aspirate 19 that is separated into layers; the second bone marrow aspirate 23 is cultured along with a culture medium and first stem cells are fixed on a bottom surface of a first culturing vessel; when a total surface area of the first stem cells reaches a first target ratio with respect to the bottom surface area of the first culturing vessel, the first stem cells are extracted from the first culturing vessel; top layer second stem cells are extracted from the first stem cells that are separated into layers and the second stem cells are cultured along with a culture medium, and the second stem cells are fixed on a bottom surface of a second culturing vessel; and, when a total surface area of the second stem cells reaches a second target ratio with respect to the bottom surface area of the second culturing vessel, the second stem cells are extracted from the second culturing vessel. (Figure 4)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015238349 | 2015-12-07 | ||
JP2016218144A JP6153653B2 (en) | 2015-12-07 | 2016-11-08 | Stem cell culture method |
PCT/JP2016/086206 WO2017099067A1 (en) | 2015-12-07 | 2016-12-06 | Stem cell culturing method |
Publications (1)
Publication Number | Publication Date |
---|---|
MY188404A true MY188404A (en) | 2021-12-07 |
Family
ID=59014159
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MYPI2018700248A MY188404A (en) | 2015-12-07 | 2016-12-06 | Stem cell culturing method |
Country Status (2)
Country | Link |
---|---|
MY (1) | MY188404A (en) |
WO (1) | WO2017099067A1 (en) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4542409B2 (en) * | 2004-10-14 | 2010-09-15 | オリンパス株式会社 | Method for culturing bone marrow-derived mesenchymal stem cells |
CN102791276B (en) * | 2010-03-10 | 2015-03-04 | 智再如股份有限公司 | Cell preparation containing mesenchymal stem cells, and method for producing same |
WO2015021189A1 (en) * | 2013-08-06 | 2015-02-12 | Regenerative Sciences, Llc | Bone marrow adipose portion isolation device and methods |
JP2015084686A (en) * | 2013-10-29 | 2015-05-07 | オリンパス株式会社 | Jig for cell culture, and automatic culture medium exchange system |
JP6377929B2 (en) | 2014-03-27 | 2018-08-22 | 株式会社吉田製作所 | Stem cell culture apparatus and stem cell culture method |
-
2016
- 2016-12-06 WO PCT/JP2016/086206 patent/WO2017099067A1/en active Application Filing
- 2016-12-06 MY MYPI2018700248A patent/MY188404A/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2017099067A1 (en) | 2017-06-15 |
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