KR970020168A - How to remove cells from fermentation broth through membrane - Google Patents

How to remove cells from fermentation broth through membrane Download PDF

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Publication number
KR970020168A
KR970020168A KR1019960045322A KR19960045322A KR970020168A KR 970020168 A KR970020168 A KR 970020168A KR 1019960045322 A KR1019960045322 A KR 1019960045322A KR 19960045322 A KR19960045322 A KR 19960045322A KR 970020168 A KR970020168 A KR 970020168A
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South Korea
Prior art keywords
fermentation broth
membrane
cells
present
increase
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KR1019960045322A
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Korean (ko)
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KR100452172B1 (en
Inventor
도시히꼬 아베
다이스께 시라이
히데또 우라사와
마고또 호시노
귀 빌레뜨
끌로드 떼라꼴
도시야 다나베
도루 나카무라
Original Assignee
노미즈 시게까쯔
트윈버드 고교 가부시키가이샤
말레 필립쁘
시에백
이나모리 쥰스케
아지노모토 가부시키가이샤
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Priority claimed from JP7265521A external-priority patent/JPH09103506A/en
Priority claimed from FR9512599A external-priority patent/FR2740049A1/en
Application filed by 노미즈 시게까쯔, 트윈버드 고교 가부시키가이샤, 말레 필립쁘, 시에백, 이나모리 쥰스케, 아지노모토 가부시키가이샤 filed Critical 노미즈 시게까쯔
Publication of KR970020168A publication Critical patent/KR970020168A/en
Application granted granted Critical
Publication of KR100452172B1 publication Critical patent/KR100452172B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

막을 사용하여 에스케리키아(Escherichia) 속에 포함되는 미생물을 배양하여 수득한 발효 브로쓰로부터 세포를 분리하는 경우, 보다 온화한 전처리 조건하에 막투과율을 개선하는 방법이 개시되어 있다.A method for improving membrane permeability under milder pretreatment conditions when a cell is used to isolate cells from fermentation broth obtained by culturing microorganisms contained in Escherichia is used.

본 발명은 폴리에틸렌이민을 발효 브로쓰에 가하고, 이어서 막을 통해 세포를 분리함을 특징으로 하여, 막을 사용하여 상술된 발효 브로쓰로부터 세포를 제거하는 방법에 관한 것이다.The present invention relates to a method for removing cells from the fermentation broth described above using a membrane, by adding polyethyleneimine to the fermentation broth and then separating the cells through the membrane.

본 발명의 발명은 본 발명에서와 같은 처리가 수행되지 않는 경우와 비교하여 막을 사용하여 발효 브로쓰에서 세포의 제거시 막 투과율을 1.5 내지 4.0배 증가시킬 수 있다. 이로써 대규모의 장비를 사용하지 않고도 막 투과율을 용이하게 증가시키고 막 투과 장비의 효능을 증가시킬 수 있다.The invention of the present invention can increase the membrane permeability by 1.5 to 4.0 times upon removal of cells from the fermentation broth using membranes as compared to the case where no treatment as in the present invention is performed. This can easily increase the membrane permeability and increase the efficacy of the membrane permeation equipment without the use of large scale equipment.

Description

막을 통해 발효 브로쓰로부터 세포를 제거하는 방법How to remove cells from fermentation broth through membrane

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

폴리에틸렌이민을 발효 브로쓰에 가한후 막을 통해 혼합물을 여과하여 세포를 분리함을 특징으로 하여, 막을 통해 에스케리키아 속에 포함되는 미생물을 배양하여 수득한 발효 브로쓰로부터 세포를 분리시켜 이를 제거하는 방법.Polyethyleneimine is added to the fermentation broth, and the mixture is filtered through the membrane to separate the cells. . 폴리에틸렌이민을 발효 브로쓰에 가하고 발효 브로쓰의 pH를 3 내지 7로 조절한 후 혼합물을 막을 통해 여과하여 세포를 분리함을 특징으로 하여, 막을 통해 에스케리키아 속에 포함되는 미생물을 배양하여 수득한 발효 브로쓰로부터 세포를 분리시켜 이를 제거하는 방법.Polyethyleneimine is added to the fermentation broth and the pH of the fermentation broth is adjusted to 3-7, and the mixture is filtered through a membrane to separate the cells. A method of separating and removing cells from fermentation broth. 제1항에 또는 제2항에 있어서, 사용된 막이 한외여과 막 또는 미세여과 막인 방법.The method according to claim 1 or 2, wherein the membrane used is an ultrafiltration membrane or a microfiltration membrane. 제1항 내지 제3항 중의 어느 한 항에 있어서, 첨가된 폴리에틸렌이민의 양이 발효 브로쓰를 기준으로 하여 0.0005 내지 0.5중량%인 방법.The process according to any one of claims 1 to 3, wherein the amount of polyethyleneimine added is 0.0005 to 0.5% by weight, based on the fermentation broth. 제1항 내지 제4항 중의 어느 한 항에 있어서, 발효 브로쓰가 폴리에틸렌이민의 첨가전, 후 또는 전후에 50 내지 130℃에서 가열되는 방법.The process according to claim 1, wherein the fermentation broth is heated at 50 to 130 ° C. before, after or before the addition of polyethyleneimine. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960045322A 1995-10-13 1996-10-11 How to remove cells from a fermentation broth through a membrane KR100452172B1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP95-265275 1995-10-13
JP7-265521 1995-10-13
JP7-10856 1995-10-13
JP26527595 1995-10-13
JP7265521A JPH09103506A (en) 1995-10-13 1995-10-13 Infrared therapeutic unit
FR9512599A FR2740049A1 (en) 1995-10-20 1995-10-20 RIGID TUBULAR CORE FILTER CARTRIDGE
FR95-12599 1995-10-20

Publications (2)

Publication Number Publication Date
KR970020168A true KR970020168A (en) 1997-05-28
KR100452172B1 KR100452172B1 (en) 2005-04-06

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