KR930702072A

KR930702072A - Sample pipette metrology

Info

Publication number: KR930702072A
Application number: KR1019930701367A
Authority: KR
Inventors: 더블유.브렌츠 챨스
Original assignee: 챨스 엠. 브록; 애보트 래보러토리즈
Priority date: 1990-11-09
Filing date: 1991-11-08
Publication date: 1993-09-08
Also published as: AU652014B2; EP0556336A4; JPH06501558A; JP3065100B2; CA2095152A1; CA2095152C; WO1992008545A1; AU9075891A; EP0556336A1

Abstract

시험 샘플에 존재하는 유체의 수준을 측정하는 비-침투성 방법. 용기(22)중의 시험 샘플에 존재하는 유체의 수준은 피펫터(1)를 샘플 표면을 향해 이동시키면서 공기를 흡인시키고 초래된 압력 변화를 검출기(18)를 사용하여 조사함으로써 측정한다. 샘플의 불균질성은 본 발명을 사용하여 샘플을 흡인하면서 검출할 수 있다.A non-invasive method of measuring the level of fluid present in a test sample. The level of fluid present in the test sample in the vessel 22 is measured by sucking air while moving the pipette 1 towards the sample surface and investigating the resulting pressure change using the detector 18. The heterogeneity of the sample can be detected by aspirating the sample using the present invention.

Description

Sample pipette metrology

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 압력(10비트 이진수로써 나타낸 +1psi로부터 -1psi까지)을 주기적인 간격으로 읽은 일련의 압력 판독 번호에 대하여 플로팅한, 본 발명의 수준 측정 방법을 사용한 정상 송아지 혈청의 대표적인 샘플의 그래프이다. 압력에서의 변화는 피펫 선단이 샘플의 표면에 닿고 공기 흡인이 즉시 정지될 때 일어난다. 제2도는 압력(10비트 이진수로써 나타낸 +1psi로부터 -1psi까지)을 흡인 데이타 지점에 대해 플로팅한, 샘플 1000㎕를 흡인하기 위해 본 발명의 흡인방법 및 수준 측정 방법을 사용한 정상 송아지 혈청의 샘플의 흡인 중의 대표적인 압력 변화의 그래프이다. 모든 압력 측정 값은 샘플에서 균질성을 나타내는 예비측정된 흡인 압력 윈도우내에 있다. 제3도는 압력(10비트 이진수로써 나타낸 +1psi로부터 -1psi까지)을 흡인 데이타 지점에 대해 플로팅한, 샘플 1000㎕를 흡인하기 위해 본 발명의 흡인 방법 및 유체 수준 측정 방법을 사용한 표면에 포말을 함유하는 정상 송아지 혈청의 대표적인 샘플의 그래프이다. 측정한 압력 값의 적어도 하나는 예비측정한 흡인 압력 윈도우의 외부에 있고, 이는 샘플의 불균질성, 즉 포말의 존재를 지시한다.1 is a graph of representative samples of normal calf serum using the leveling method of the present invention, plotted against a series of pressure readings read at periodic intervals of pressure (from +1 psi to -1 psi expressed as 10-bit binary). . The change in pressure occurs when the pipette tip touches the surface of the sample and the air suction immediately stops. Figure 2 shows a sample of normal calf serum using the aspiration method and level measurement method of the present invention to aspirate 1000 μl of the sample, plotting the pressure (from +1 psi to -1 psi expressed as 10-bit binary) against the aspiration data point. It is a graph of representative pressure change during aspiration. All pressure measurements are within a premeasured suction pressure window that shows homogeneity in the sample. Figure 3 contains foam on the surface using the aspiration method and fluid level measurement method of the present invention to aspirate 1000 μl of sample, plotting pressure (+1 psi to -1 psi represented as 10-bit binary) against a suction data point. Is a graph of a representative sample of normal calf serum. At least one of the measured pressure values is outside the premeasured suction pressure window, which indicates the heterogeneity of the sample, ie the presence of foam.

Claims

(a) (i) measuring the ambient air pressure in the pipette as a reference pressure reading; (Ii) aspirating air with the pipette when moving the pipette towards the fluid sample in the vessel; (Iii) measuring the level of fluid in the container, including investigating changes in air pressure in the pipette to indicate the surface level of the fluid in the container; (b) soaking the fluid from the vessel into the pipette in a controlled step while immersing the pipette in the fluid and sucking the fluid in an increased amount; (c) investigate the pressure change after each incremental aspiration step; (d) pipette metering fluid from the vessel, comprising moving the tip of the pipette away from the sample in a manner to remove all droplets.

The method of claim 1, wherein the tip of the pipette is disposable.

The method of claim 1 wherein the tip of the pipette is reusable.

(a) measuring the ambient air pressure in the pipette as a reference pressure reading; (b) aspirating air with the pipette when moving the pipette towards the fluid sample in the vessel; (c) examining the air pressure in the pipette to measure the simple surface level in the vessel by the amount of change in air pressure; (d) immersing the pipette into the sample and drawing a volume of sample from the vessel into the pipette; (e) measuring the air pressure in the pipette immediately after the aspiration of step (d) and after the pressure has reached a steady state; (f) comparing the measured pressure of step (e) with a premeasured suction pressure window; (g) observing a pressure value belonging to the outside of the window, wherein the method detects a heterogeneity in a fluid sample.

The method of claim 4, wherein the heterogeneity in the sample is air bubbles.

The method of claim 4, wherein the heterogeneity in the sample is foamy.

The method of claim 4, wherein the heterogeneity in the sample is an aggregate.

(a) measuring the ambient air pressure in the pipette as a reference pressure reading; (b) immersing the pipette in the fluid sample and drawing a volume of the sample into the pipette; (d) comparing the measured pressure of step (c) with a premeasured suction pressure window; (e) detecting a heterogeneity in the fluid sample, comprising observing a pressure value belonging to the outside of the window.

(a) measuring the ambient air pressure in the pipette as a reference pressure reading; (b) aspirating air with the pipette when moving the pipette towards the fluid sample in the vessel; (c) investigating the air pressure in the pipette to indicate the surface level of the sample in the container by a change in air pressure.

※ Note: The disclosure is based on the initial application.