KR880011340A - 하이브리드 세르핀 및 이들이 암호화 dna - Google Patents
하이브리드 세르핀 및 이들이 암호화 dna Download PDFInfo
- Publication number
- KR880011340A KR880011340A KR1019880002920A KR880002920A KR880011340A KR 880011340 A KR880011340 A KR 880011340A KR 1019880002920 A KR1019880002920 A KR 1019880002920A KR 880002920 A KR880002920 A KR 880002920A KR 880011340 A KR880011340 A KR 880011340A
- Authority
- KR
- South Korea
- Prior art keywords
- hls2
- exon
- gene
- hybrid
- serpin
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8121—Serpins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8121—Serpins
- C07K14/8125—Alpha-1-antitrypsin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
내용없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 hLS2 유전자 구조의 다이어그램.
제2도는 및 2a도;hLS2의 엑손 1내지 4를 포함하는 하이브리드 세르핀 유전자의 작제도.
Claims (12)
- 인간 루세르핀-2(hLS2)의 유전자 구조를 포함하는 엑손에 실질적으로 상응하는 아미노산 일부-서열을 포함하는 하이브리드 세르핀.
- 제1항에 있어서, hLS2-α1-안티트립신 또는 앤지오텐시노겐의 엑손에 상으하는 아미노산 일부-서열을 포함하는 하이브리드 세르핀.
- hLS2의 엑손/인트론 구조를 가지며 세르핀을 암호화 하는 적어도 두 개의 유전자로부터 유래된 엑손으로부터 hLS2에 상응하는 유전자 구조를 갖는 유전자를 재조합한 다음, 이 재조합 유전자를 숙주세포내에서 발현시킴을 특징으로 하여, 이 하이브리드 세르핀을 제조하는 방법.
- 제3항에 있어서, 숙주세포가 고등 진핵세포인 방법.
- hLS2에 상응하는 유전자 구조가 포함된 세르핀 유전자의 엑손을 포함하는, hLS2에 상으하는 유전자 구조를 갖는 재조합 유전자.
- 제5항에 있어서, 엑손이 관련 인트론의 스폴리스 시그널과 분지점에 의해 플랭킹 되는 유전자.
- hLS2의 엑손을 포함하는 게놈성 DNA 단편.
- hLS2의 게놈성 DNA.
- 제5항 내지 7항중 어느 한항에 따른 DNA 형질 감염된 숙주세포.
- 제1항 또는 2항에 따른 하이브리드 세르핀을 함유하는 약제.
- 제7항 또는 8항에 따른 게놈성 DNA의 전부 또는 일부를 함유하는 진단용 제제.
- 인간 DNA를 제7항 또는 8항에 따른 DNA 또는 상응하는 RNA와 하이브리드화시킴을 특징으로 하는 진단방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP3709255.3 | 1987-03-20 | ||
DE19873709255 DE3709255A1 (de) | 1987-03-20 | 1987-03-20 | Hybridserpine und dafuer kodierende dna |
Publications (1)
Publication Number | Publication Date |
---|---|
KR880011340A true KR880011340A (ko) | 1988-10-27 |
Family
ID=6323634
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019880002920A KR880011340A (ko) | 1987-03-20 | 1988-03-19 | 하이브리드 세르핀 및 이들이 암호화 dna |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0283932A3 (ko) |
JP (1) | JPS63267294A (ko) |
KR (1) | KR880011340A (ko) |
AU (1) | AU611676B2 (ko) |
DE (1) | DE3709255A1 (ko) |
DK (1) | DK151788A (ko) |
FI (1) | FI881268A (ko) |
HU (1) | HUT46740A (ko) |
IL (1) | IL85789A0 (ko) |
PT (1) | PT87009B (ko) |
ZA (1) | ZA881948B (ko) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09504174A (ja) * | 1993-10-29 | 1997-04-28 | インサイト ファーマシューティカルズ,インク. | プロテアーゼネキシン1変異体を含むキメラ蛋白 |
DE19742725A1 (de) * | 1997-09-26 | 1999-04-01 | Abts Harry Frank Dr | Hurpin/PI13: Ein neuer, UV-reprimierbarer, Serin Protease Inhibitor aus der Familie der Ovalbumin-Serpine |
US7247704B2 (en) | 2000-12-18 | 2007-07-24 | Arriva Pharmaceuticals, Inc. | Multifunctional protease inhibitors and their use in treatment of disease |
DE60132632T2 (de) * | 2000-12-18 | 2009-01-22 | Arriva Pharmaceuticals, Inc., Alameda | Multifunktionelle protease-inhibitoren und deren verwendung zur behandlung von krankheiten |
EP1903113A1 (en) * | 2000-12-18 | 2008-03-26 | Arriva Pharmaceuticals, Inc. | Multifunctional protease inhibitors and their use in treatment of disease |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3521226A1 (de) * | 1985-02-08 | 1986-08-14 | Hoechst Ag, 6230 Frankfurt | Gene fuer biologisch aktive proteine |
-
1987
- 1987-03-20 DE DE19873709255 patent/DE3709255A1/de not_active Withdrawn
-
1988
- 1988-03-17 EP EP88104248A patent/EP0283932A3/de not_active Withdrawn
- 1988-03-17 FI FI881268A patent/FI881268A/fi not_active Application Discontinuation
- 1988-03-18 DK DK151788A patent/DK151788A/da not_active IP Right Cessation
- 1988-03-18 PT PT87009A patent/PT87009B/pt not_active IP Right Cessation
- 1988-03-18 IL IL85789A patent/IL85789A0/xx unknown
- 1988-03-18 ZA ZA881948A patent/ZA881948B/xx unknown
- 1988-03-18 AU AU13288/88A patent/AU611676B2/en not_active Ceased
- 1988-03-18 HU HU881365A patent/HUT46740A/hu unknown
- 1988-03-19 JP JP63067041A patent/JPS63267294A/ja active Pending
- 1988-03-19 KR KR1019880002920A patent/KR880011340A/ko not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
AU1328888A (en) | 1988-09-22 |
PT87009A (pt) | 1988-04-01 |
FI881268A0 (fi) | 1988-03-17 |
PT87009B (pt) | 1992-06-30 |
DE3709255A1 (de) | 1988-09-29 |
AU611676B2 (en) | 1991-06-20 |
JPS63267294A (ja) | 1988-11-04 |
ZA881948B (en) | 1988-12-28 |
HUT46740A (en) | 1988-11-28 |
EP0283932A3 (de) | 1989-10-25 |
DK151788A (da) | 1988-09-21 |
DK151788D0 (da) | 1988-03-18 |
IL85789A0 (en) | 1988-09-30 |
EP0283932A2 (de) | 1988-09-28 |
FI881268A (fi) | 1988-09-21 |
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