KR20240117721A - Composition for feminine cleanser containing natural extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for feminine cleanser containing natural extracts as active ingredients, which is non-irritating, effective in preventing diseases related to the female genitourinary system, and can increase convenience of use.

Description

Composition for feminine cleanser containing natural extract as an active ingredient {COMPOSITION FOR FEMININE CLEANSER CONTAINING NATURAL EXTRACT AS AN ACTIVE INGREDIENT}

The present invention relates to a composition for feminine wash containing natural extracts as active ingredients.

The female genitourinary system consists of the ovaries, fallopian tubes, uterus, cervix, vagina, and vulva. Female genitourinary system diseases include pelvic inflammatory disease, endometriosis, ovarian cyst, polycystic ovary syndrome, ovarian cancer, uterine fibroids, uterine polyps, retroflexed uterus, Uterine and vaginal prolapse, uterine cancer, trophoblastic carcinoma, cervical erosion, cervical dysplasia, cervical cancer, vulvovaginitis, vaginal mycosis, bacterial vaginitis, Bartholinitis, cancer of the vulva and vagina, sexually transmitted diseases (gonorrhea, chlamydia pelvic inflammatory disease, syphilis, trichomoniasis) It is reported that these diseases are relatively common and most women experience at least one disease during their lifetime.

In the female genitourinary system, lactic acid bacilli exist, which maintain the pH of the genitourinary system at a slightly acidic level of 4.5 to 6.5, which plays a role in preventing the proliferation of harmful bacteria. However, in addition to physical damage and hormonal imbalance, an unclean urogenital environment and bacterial contamination due to sexual intercourse Diseases related to the female genitourinary system can easily occur due to diseases or sexually transmitted diseases. In other words, the vagina and vulva are directly exposed to the external environment due to the functional characteristics of the structure of the female genitourinary system, and provide a conditional environment in which various bacterial groups such as sheep, temperature, and humidity are cultured, causing bacterial infection and bacterial vaginosis.

Under normal conditions, vaginal flora provides a mildly acidic environment that helps protect against invasion by pathogenic microorganisms. Normal vaginal pH is 4.5 to 5.1. The reason why the inside of the vagina is acidic is because vaginal bacteria and vaginal epithelial cells produce lactic acid using glycogen and glucose. Lactic acid bacteria living in the vagina play a useful role in suppressing the growth of harmful bacteria by producing lactic acid and maintaining pH.

However, under normal conditions as above, the vaginal flora, that is, vaginal balance, can be easily overturned by various external factors that ultimately lead to vaginal infection. Vaginal infection is a clinical syndrome, and mainly bacterial vaginosis, candida vaginitis, and trichomonas vaginitis are reported, of which the most common vaginitis is bacterial vaginosis.

The bacterial vaginosis is reported to be mainly caused by an increase in the number of anaerobic organisms accompanied by a decrease in lactic acid bacteria in the vagina. In addition, it can also be caused by bacterial or viral infection, taking antibiotics or birth control pills, or using excessive vaginal cleansers. It is reported that there is. A decrease in the number of lactic acid bacteria in the vagina reduces competition for nutrients, reduces the amount of lactic acid present, increases pH, and increases the growth of opportunistic pathogens, the growth of which is normally inhibited in the vagina. This happens and causes the disease to develop.

It is a feminine cleanser or vaginal cleanser that is effective in preventing diseases related to the female genitourinary system, is hypoallergenic, and is easy to use. It is more hypoallergenic than commonly used soaps and body cleansers, and contains functional natural ingredients to create a healthier environment when used. There is a need to develop a feminine cleanser that can be provided.

KR 10-1922657 B1

The purpose of the present invention is to provide a composition for feminine wash containing natural extracts as active ingredients.

Another object of the present invention is to provide a composition for feminine cleanser containing a natural extract as an active ingredient, which is non-irritating, effective in preventing diseases related to the female genitourinary system, and has increased convenience of use.

In order to achieve the above object, the present invention is a composition for feminine cleanser containing natural extracts as active ingredients, purified water; Wormwood extract; And it may include a tree extract.

The composition may be characterized by excellent skin moisturizing, pH control, and antibacterial effects.

The composition further includes a natural extract, and the natural extract may be selected from the group consisting of Gujeolcho extract, tangerine peel extract, and mixtures thereof.

The composition may include 20 to 40 parts by weight of mugwort extract, 10 to 20 parts by weight of G. dandelion tree extract, 1 to 5 parts by weight of Gujeolcho extract, and 1 to 5 parts by weight of tangerine peel extract, based on 100 parts by weight of purified water.

The extract may be extracted using an extraction solvent selected from the group consisting of water, alcohols with 1 to 6 carbon atoms, and mixtures thereof.

The composition is used by applying it to the skin, and after application, the vaginal pH can be maintained at 4.5 to 5.5.

The composition is excellent in improving vaginal-related diseases.

The present invention contains natural extracts as active ingredients, is non-irritating, is effective in preventing diseases related to the female genitourinary system, and can increase convenience of use.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

The vagina is a passage connecting the vulva and the uterus and has the highest temperature and humidity in the female body, so it is easy for harmful strains, including bacteria and fungi that can cause vaginitis, to proliferate, resulting in a high possibility of disease.

Under normal conditions, many lactic acid bacteria live inside the vagina and make the inside of the vagina slightly acidic, suppressing the growth of pathogens and molds. However, women who take antibiotics for a long time, use birth control pills for a long time, use tampons or loops, or are pregnant, give birth, breast-feeding, or go through menopause. When the balance inside the vagina is broken for reasons such as changes in hormonal balance, opportunistic pathogens, the growth of which is normally suppressed under normal conditions, proliferate within the vagina, causing vaginitis. Therefore, it is advisable to always keep the vagina clean and be careful not to expose it to bacterial infections from the outside.

Meanwhile, the female reproductive organs can easily be infected with germs through the mucous membrane during sexual activity. In addition, when a contraceptive device is installed in the uterus for a long period of time, chronic inflammation is caused, and the mucous membrane of the relevant body part is often damaged when a medical device is introduced into the body or when giving birth to a mammal. These wounds are an opportunity for infection by pathogens. may increase and cause inflammation. Additionally, when there is a lot of bleeding before or after menstruation, not only does it feel very hygienically uncomfortable, but the chance of infection increases. Therefore, it is very important to reduce fatal complications through appropriate prevention, diagnosis and treatment.

In women, inflammation of the genitourinary system is one of the most common diseases among patients visiting gynecologists, and this inflammation is very diverse, ranging from bacterial to parasitic and viral causes. These patients are accompanied by itching, severe inflammation, and bad odor. In particular, human papilloma virus infection is closely related to cervical cancer, so careful attention is required.

To prevent such female diseases and remove unpleasant odors, vaginal cleansers (cleansers) and vulvar cleansers are often used. These cleansers cannot avoid contamination by microorganisms during the manufacturing process and use, which reduces the product's preservation power. Preservatives are used for improvement purposes.

Preservatives previously used for purposes such as vaginal cleansers contain chemicals as their main active ingredients. It has excellent antibacterial properties and prevents deterioration caused by various bacteria, fungi, and bacteria, and has excellent preservation power. However, it can often cause toxicity, skin irritation, and allergies.

The present invention relates to a composition for feminine cleanser that can be used as a vaginal cleanser without problems with side effects such as toxicity, skin irritation, and allergies.

Specifically, purified water; Wormwood extract; And it may include a tree extract.

The above-mentioned mugwort (Artemisia Linne) is reported to have about 200 species in the northern hemisphere and 38 species distributed in Korea. Representative examples include mugwort, water mugwort, mountain mugwort, perennial mugwort, yellow sea mugwort, human mugwort, mugwort, and bush mugwort. The petioles are medicinal, the young leaves are edible, and the regular leaves are used to make moxa. The white fur is scraped and used to make ink, and is also called Baekho, Bongae, Aecho, or Bongho. Mugwort is used as a hemostatic agent for nosebleeds, uterine bleeding, etc. in traditional medicine, and is effective for digestion, lower abdominal pain, deworming, and odor removal. It is said to be effective in treating gastrointestinal diseases, constipation, neuralgia, cold diseases, gynecological diseases, and asthma, and is drunk as mugwort tea. It has been done.

The Gorosoe tree is a deciduous tree belonging to the maple family and grows naturally at altitudes of 100 to 1,800 m and is distributed throughout Korea, Japan, China, and Manchuria. Korea's Acer pictum subsp. mono, red A. mono for. rubrieps, and A. okamotoanum trees have strong cold tolerance and grow naturally in Jiri Mountain, Baekun Mountain, Jogyesan Mountain, and Gangwon-do. ), A. truncatum, A. mono for. dissectum, A. mono var. savatieri, A. mono var. horizontale, A. mono for. horizontale), A. mono for. dissectum, A. mono var. horizontale, Nine species and varieties are known to be growing, including A. mono for. connivens) and A. mono var. ambiguum.

The name Gorosoe comes from the Chinese word golissu, which means it is good for bones. In Oriental medicine, the juice that flows out from cutting a tree is called pungdang and is used as medicinal water for patients with gastrointestinal diseases, lung diseases, neuralgia, and arthritis. As such, the history of drinking the sap of the Gorosoe tree has a long history, and in recent years, sap consumption has increased, so only research on the sap has been conducted.

In the present invention, the Sorosoe tree extract relates to a Sorosoe tree leaf extract using the Sorosoe leaves. Previously, research was conducted on the antioxidant effect of Gorosoe tree sap or Gorosoe tree leaf extract, but no examples of its use as a composition for feminine cleanser as in the present invention were confirmed.

In the present invention, it is characterized in that it provides a composition for feminine cleanser containing mugwort extract and Gorosoe tree leaf extract, when the mugwort extract and Gorosoe tree leaf extract are used in combination as a complex extract compared to when used alone. , it was confirmed that it was highly effective as a vaginal cleanser.

The mugwort extract or Gorosoe tree leaf extract is a natural extract, extracted using an extraction solvent, specifically, extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof. It will happen.

Specifically, to produce a natural extract, there are steps of washing the natural product; Drying after washing; Grinding the natural product after drying; leaching the pulverized material using an organic solvent; Leaching and drying the sample; Leaching using water; And including the step of leaching, a natural extract can be obtained.

The extract extracted using the organic solvent may further include the step of performing fractionation using the organic solvent, and the method of preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction. You can.

Specifically, it may be an extract obtained by extracting a natural extract from which foreign substances have been removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract obtained by sequentially applying the solvents to the sample.

The reflux extraction method is based on 100 mL of water and alcohol with 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, reflux time of 1 to 3 hours, and 50 to 100% of alcohol or water with 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol with 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, reflux time of 1 to 2 hours, and 70 to 90% of alcohol or water with 1 to 4 carbon atoms. .

The leaching method is carried out at 15 to 30°C for 24 to 72 hours, and water or 50 to 100% alcohol with 1 to 6 carbon atoms is used as the extraction solvent. More specifically, it is carried out at 20 to 25°C for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The ultrasonic extraction method proceeds at 30 to 50°C for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol with 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50°C for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The extraction solvent can be used in an amount of 2 to 50 times, more specifically, 2 to 20 times the weight of the sample. For extraction, the sample may be left in the extraction solvent for 1 to 72 hours, more specifically, 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a new fractionation solvent. The fractionating solvent used during fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride. After obtaining the extract or fraction, additional methods such as concentration or freeze-drying can be used.

The composition further includes a natural extract, and the natural extract may be selected from the group consisting of Gujeolcho extract, tangerine peel extract, and mixtures thereof.

Preferably, the natural extract is Gujeolcho extract and tangerine peel extract, and when used by mixing with the mugwort extract and Gorosoe tree leaf extract, when used as a feminine cleanser, the effect increases due to the interaction between the natural extracts.

The Siberian chrysanthemum is a plant belonging to the genus Asteraceae and Chrysanthemum.　It is a perennial herb that grows widely in the mountains and fields of Japan, China, and Korea. Gujeolcho extends its underground rhizome to the surrounding area. The stem grows straight to a height of about 50cm, and the root leaves and leaves at the bottom of the stem are egg-shaped, straight at the bottom, and deeply split. There are usually four forked pieces, each with fine sawtooth edges. From September to October, flowers bloom one at a time toward the sky at the ends of stems or branches. When it first blooms, it is a slightly dark pink color, but gradually turns white. Like the flowers of the Asteraceae family, it is fragrant and beautiful, so it is widely cultivated for ornamental purposes and is also used as an herbal medicine. It is said to be effective in resolving indigestion and is used to treat menstrual irregularity, cold uterus, infertility, cold stomach, and indigestion. Sangujeolcho, a similar species, grows on the top of deep mountains in various places, and Rockygujeolcho grows in the northern alpine areas, but their uses are all similar.

The tangerine peel is the peel of the mature fruit of Citrus reticulata Blanco, Citrus unshiu Markor., Citrus sinensis (L.) Osbeck, or a variety thereof, which belongs to the Rutaceae family. .

Although the extract of Gujeolcho extract and tangerine peel extract are contained in small amounts compared to other natural extracts, when used as a composition for feminine cleanser, they are characterized by increasing skin moisturization, pH adjustment, and antibacterial effect.

The composition may include 20 to 40 parts by weight of mugwort extract, 10 to 20 parts by weight of G. dandelion tree extract, 1 to 5 parts by weight of Gujeolcho extract, and 1 to 5 parts by weight of tangerine peel extract, based on 100 parts by weight of purified water. Within the above range, when used as a composition for feminine cleanser, skin moisturizing, pH control, and antibacterial effects can be increased. The composition is used by applying it to the skin, and the vaginal pH can be maintained at 4.5 to 5.5 after application. .

As previously explained, the normal vaginal pH is 4.5 to 5.1, and maintaining this pH can play a useful role in suppressing the growth of harmful bacteria. Accordingly, the composition for feminine cleanser of the present invention is characterized by adjusting pH to a normal range by simply applying it to the skin.

The composition is excellent in improving vaginal-related diseases. Specifically, the vaginal-related disease includes vaginal infection or inflammatory conditions characterized by symptoms such as vaginal discharge, odor, burning, itching, dyspareunia, and urinary pain.

Vaginal atony is a condition in which the muscles around the vagina and the vaginal entrance muscles sag, the vaginal mucosa slows down, and the fascia relaxes. As the elasticity of the vagina decreases, sexual sensation is weakened, leading to frigidity during sexual intercourse. In severe cases, it can lead to a lack of confidence as a woman. It comes to a situation.

Vaginal dryness is a phenomenon in which vaginal secretions that should normally come out are reduced due to a constitution that lacks vaginal fluid or a decrease in blood flow and circulation around the uterus and vagina. It mainly occurs when the epithelial cells of the vagina and urethra lose their elasticity and flexibility and become thinner when female hormones decrease. do. If vaginal dryness continues for a long time, your immunity may decrease and you may be exposed to various diseases, and there is a risk of urinary incontinence and uterine prolapse due to decreased contractility.

Vaginal mycosis is a vaginitis caused by Candida bacteria. It is one of the most common vaginitis that causes inflammation when Candida colonizes the moist genital area. The main symptom is increased coldness, an inflammatory reaction on the skin, and itching. Additionally, there are differences such as peeling of the skin or discharge of pus depending on the type and degree of the disease, secondary bacterial infection, etc.

Coldness is a type of circulatory disorder caused by poor blood circulation in the entire body or part of the body, and is a symptom in which the amount of cold secreted in the female genitals increases. The reason why this secretion occurs is a natural phenomenon to protect the genitals from various harmful bacteria, but when the amount of the secretion increases, it gets stuck on the underwear, which is always humid and damp, causing an odor. It is the most common cause of vaginitis. susceptible to candida albicans.

The present invention is for the prevention and treatment of the diseases described above, and can be used for the prevention and treatment of all diseases related to the vagina, and is not limited to the above examples.

Manufacturing Example 1

Preparation of composition for feminine wash

1. Preparation of natural extract

Mugwort was washed, dried, and then ground. 80 g of the ground product was added to 300 mL of ethanol and thoroughly mixed using a stirring magnetic bar at 60°C for 2 hours.

After centrifugation at 10,000 RPM for 10 minutes, the supernatant was taken, 300 mL of ethanol was added, and the mixture was further mixed at 60°C for 1 hour. The supernatant was collected again and partially purified through a filtration filter.

It was prepared by completely drying the raw extract by applying a vacuum to an oven with an internal temperature set to 45°C using a concentrator.

Gorosoe tree leaf extract, Gujeolcho extract, and tangerine peel extract were also prepared in the same manner.

2. Preparation of composition for feminine wash

The mugwort extract, Gorosoe tree leaf extract, Gujeolcho extract, and tangerine peel extract were mixed with purified water as shown in Table 1 below to prepare a composition for feminine cleanser.

Hn1 Hn2 Hn3 Hn4 Hn5 Hn6 Hn7 Hn8 Hn9 Purified water 100 100 100 100 100 100 100 100 100 Mugwort 30 - 10 30 50 30 30 30 30 coriander tree leaves - 30 10 30 50 30 30 30 30 Gujeolcho - - - - - One 3 5 10 tangerine peel - - - - - One 3 5 10

(Unit: parts by weight)

Experimental Example 1

Skin moisturizing effect experiment

In order to evaluate the moisturizing effect of the composition for feminine cleanser, Epsilon E100 (Biox Systems Ltd., UK) was applied to evaluate the improvement in moisturizing before and after using the composition for 1 week, 2 weeks, 3 weeks, and 4 weeks.

Specifically, after applying 10 ml of the composition for feminine cleanser to the back of the hand every day, the amount of skin moisture was measured using Epsilon E100, and the moisture calculation rate was calculated.

Moisture improvement rate (%)= [(measured value after use - measured value before use)/measured value before use]*100

In Epsilon E100, the higher the skin moisture content, the higher the brightness of the image results.

The experimental results are shown in Table 2 below.

Hn1 Hn2 Hn3 Hn4 Hn5 Hn6 Hn7 Hn8 Hn9 Before use 0 0 0 0 0 0 0 0 0 1 week 10.22 10.85 11.48 14.58 12.46 13.46 17.48 18.56 12.64 2 weeks 11.24 11.54 12.43 22.48 13.12 18.88 25.46 27.64 15.46 3 weeks 10.21 11.48 12.41 36.64 12.89 19.49 40.42 41.58 17.48 4 weeks 10.14 11.24 13.58 48.47 12.88 19.88 50.24 52.21 20.21

According to the above experimental results, it can be confirmed that the moisturizing effect is improved for both Hn1 to Hn9, but in particular, as in the present invention, mugwort extract, Gorosoe tree leaf extract, Gujeolcho extract and tangerine peel extract are all included, and the moisturizing effect of the present invention is improved. It was confirmed that, when included within the range, a better moisture improvement effect appears due to the synergistic effect of mixing natural extracts.

Experimental Example 2

pH control effect experiment

Eighty women aged 20 to 40 who were patients with vaginitis infected with Candida albicans were selected and allowed to use the T1 feminine cleanser composition for 2 weeks to test changes in vaginal pH.

When Hn 3 was used consistently for 2 weeks, it was confirmed that the pH changed from 8.1 to 7.24 after 1 week and to 5.5 after 2 weeks.

Likewise, when Hn 8 was used consistently for 2 weeks, the pH was confirmed to change from 7.57 to 6.24 after 1 week and to 5.1 after 2 weeks.

As a result of using the composition for feminine hygiene using Hn 3 and Hn 8 of the present invention for 2 weeks, the pH inside the vagina is lowered to adjust it to slightly acidic and the beneficial bacteria in the vagina grow, thereby suppressing the growth of harmful bacteria that cause vaginitis, etc. can confirm.

Experimental Example 3

Antibacterial effect experiment

Pre-cultured Candida albicans was spread on 100 ㎕ of plate medium for disc diffusion measurement (Sabouraud Dextrose Agar. Difco. USA), and then the composition for feminine cleansing Hn1 to Hn9 was spread on a sterilized paper disc. The sample was dropped at a concentration of 5 mg/disc and placed on the medium.

As a control, Itraconazole, an antifungal agent, was instilled onto a paper disk at a concentration of 100 μg/disk and then placed on the medium. After culturing this plate medium at 28°C for 48 hours, the size of the clean zone of bacteria produced after culturing was measured, and the results are shown in Table 3 below.

Growth retardation ring size (mm) Hn1 32 Hn2 30 Hn3 26 Hn4 21 Hn5 31 Hn6 32 Hn7 17 Hn8 15 Hn9 30 control group 35

As shown in Table 3, the composition for feminine hygiene of the present invention showed a stronger growth inhibition effect than the control group, itraconazole (antifungal agent).

According to the above experimental results, it can be confirmed that the composition for feminine cleanser of the present invention has excellent antibacterial effect.

Experimental Example 4

Skin irritation experiment

45 women aged 20 to 40 were selected, divided into 9 groups of 5 each, and were asked to use the composition for feminine cleansing of Hn1 to Hn9 for 10 days, and then a survey was conducted to determine whether skin irritation occurred. The results are shown in Table 4 below.

[Evaluation standard]

◎: No irritation at all

○: Almost no irritation

△: Slightly irritating, so it is not recommended to use on sensitive mucous membranes.

Х: Cannot be used due to severe irritation.

Hn1 Hn2 Hn3 Hn4 Hn5 Hn6 Hn7 Hn8 Hn9 One △ △ △ ○ ○ ○ ◎ ◎ △

According to Table 4, the composition for feminine cleanser of the present invention did not cause severe irritation in most cases, but some women with sensitive skin were evaluated to have some irritation.

On the other hand, it was confirmed that all women who used Hn 7 and Hn 8 gave excellent evaluations, saying that there was no irritation at all.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (7)

Purified water;
Wormwood extract; and
Contains cypress tree extract
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 1,
The composition is characterized by excellent skin moisturizing, pH control, and antibacterial effects.
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 1,
The composition further includes natural extracts,
The natural extract is selected from the group consisting of Gujeolcho extract, tangerine peel extract, and mixtures thereof.
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 3,
The composition comprises 20 to 40 parts by weight of mugwort extract, 10 to 20 parts by weight of Achyranthesia extract, 1 to 5 parts by weight of Gujeolcho extract, and 1 to 5 parts by weight of tangerine peel extract, based on 100 parts by weight of purified water.
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols with 1 to 6 carbon atoms, and mixtures thereof.
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 1,
The composition is used by applying it to the skin, and maintains the vaginal pH at 4.5 to 5.5 after application.
A composition for feminine cleanser containing natural extracts as active ingredients. According to paragraph 1,
The composition has excellent effects on improving vaginal diseases.
A composition for feminine cleanser containing natural extracts as active ingredients.