KR20240086759A - RCD1 gene derived from maize controlling plant drought stress resistance and uses thereof - Google Patents

Abstract

The present invention relates to the corn-derived RCD1 gene that regulates the drying stress resistance of plants and its use. More specifically, to the corn RCD1 gene consisting of the nucleotide sequence of SEQ ID NO: 1 that can confer drying resistance to plants and its use. It's about.

Description

RCD1 gene derived from maize that controls drought stress resistance of plants and uses thereof {RCD1 gene derived from maize controlling plant drought stress resistance and uses thereof}

The present invention relates to the RCD1 gene derived from corn ( Zea mays L.), which regulates drought stress resistance in plants, and its use.

Corn ( Zea mays L.) is one of the world's three largest crops, and its production and yield are affected by various biotic and/or abiotic stress factors during the growing season. In particular, drought is the most important environmental obstacle to corn production, and it has been reported that corn yields decrease by about 15% worldwide due to drought. In general, when corn is subjected to drought stress, seedling formation, vegetative growth, root development, photosynthesis, flowering, ASI, seed formation, and yield are seriously affected. In particular, if drought stress occurs during the transition from vegetative growth to reproductive growth, the release of male flowers and pollen is delayed, the budding period and the development of corn silk are delayed, and the ASI (anthesis-silking interval) increases, making fertilization impossible or embryo generation even if fertilization occurs. It is suppressed and obstructed, which is a major cause of water quantity reduction. Therefore, the development of genetic factors that provide tolerance to drying stress has great economic and social value.

Meanwhile, Korean Patent No. 2019041 discloses 'a method for determining drought tolerance in corn', and Korean Patent Publication No. 2011-0071821 discloses 'genes and transgenic plants related to drought stress resistance', but the 'drying stress resistance-related genes and transgenic plants' of the present invention are disclosed. There has been no description of the corn-derived RCD1 gene that regulates the drought stress resistance of plants and its uses.

The present invention was developed in response to the above-mentioned needs. In order to find genes that confer drying resistance in corn, the present inventors performed genotyping on various corn lines using a SNP chip provided by Illumina, and performed corn RCD1 The present invention was completed by identifying a specific SNP located inside the gene, and confirming the mutation of amino acid residues and the strengthening or weakening of drying resistance depending on the type of base sequence at the SNP position.

In order to solve the above problems, the present invention provides a corn-derived RCD1 protein consisting of the amino acid sequence of SEQ ID NO: 2.

Additionally, the present invention provides a gene encoding the RCD1 protein.

Additionally, the present invention provides a recombinant vector containing the above gene.

Additionally, the present invention provides host cells transformed with the above recombinant vector.

In addition, the present invention includes the steps of transforming a plant cell with a recombinant vector containing a corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2; and redifferentiating the transformed plant from the transformed plant cells.

Additionally, the present invention provides transgenic plants with controlled drought stress resistance prepared by the production method of the present invention and their transformed seeds.

In addition, the present invention provides drought stress resistance in plants, comprising the step of transforming plant cells with a recombinant vector containing the corn-derived RCD1 protein-encoding gene consisting of the amino acid sequence of SEQ ID NO: 2 to regulate the expression of the RCD1 protein-encoding gene. Provides a method to control .

In addition, the present invention provides a composition for regulating drought stress resistance in plants, comprising the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2 as an active ingredient.

Since the present invention has secured the corn RCD1 gene sequence, which has the function of imparting drought resistance to plants, and the SNP information related to the RCD1 gene to enhance the function of drought resistance, it is expected that the information can be usefully used in the development of crop varieties. do. In particular, it is believed that excellent corn seeds with desiccation resistance can be developed by applying recently developed genome editing technology.

Figure 1 shows the results of confirming the phenotype of drought-resistant or susceptible maize lines after treatment with drought stress.
Figure 2 shows the SNP selection process specifically related to drought stress, and only homozygous SNPs that were identical within a group and showed different characteristics between groups were finally selected. The final selected SNP is indicated by a yellow line in the figure.
Figure 3 shows the results of SNP genotyping analysis, showing information on the 133 SNPs associated with finally selected drought stress resistance or susceptibility traits.
Figure 4 shows the detailed information analysis results for the selected 133 SNPs.
Figure 5 is a structural analysis diagram of genes related to corn drying stress resistance.
Figure 6 shows the base sequence comparison results of the RCD1 gene of a dry stress-resistant maize line and the rcd1 gene of a susceptible maize line.
Figure 7 shows the results of a dry stress resistance test of Arabidopsis plants into which the RDC1 gene (SEQ ID NO: 1, T) of a dry stress-resistant maize line or the rcd1 gene (SEQ ID NO: 3, S) of a susceptible maize line was introduced.

In order to achieve the object of the present invention, the present invention provides a corn-derived RCD1 protein consisting of the amino acid sequence of SEQ ID NO: 2.

Additionally, the present invention provides a gene encoding the corn-derived RCD1 protein. The corn-derived RCD1 gene of the present invention may be composed of the base sequence of SEQ ID NO: 1, but is not limited thereto.

The RCD1 gene consisting of the base sequence of SEQ ID NO: 1 according to the present invention was isolated from a drought-resistant corn line, and is composed of the wild type corn RCD1 gene (550 to 2,292 nt of Genbank accession number: XM_008646096.3) and some bases. There is a difference. Specifically, in the nucleotide sequence of SEQ ID NO: 1 according to the present invention, the 745th, 755th, 982nd, 1,433rd, and 1,606th bases are A, A, T, A, and G, respectively, but the wild-type corn RCD1 gene has the bases at the corresponding positions. The difference is that are G, G, C, G, and A, respectively. In particular, all of the above base mutations are non-synonymous mutations, and are characterized by mutations in which amino acid residues of the corn RCD1 protein are substituted.

The present invention also provides a recombinant vector containing the corn-derived RCD1 gene consisting of the base sequence of SEQ ID NO: 1.

The term “recombinant” refers to a cell that replicates a heterologous nucleic acid, expresses a heterologous nucleic acid, or expresses a peptide, heterologous peptide, or protein encoded by a heterologous nucleic acid. Recombinant cells can express genes or gene segments that are not found in the natural form of the cell, either in sense or antisense form. Additionally, recombinant cells can express genes found in cells in their natural state, but the genes have been modified and reintroduced into the cells by artificial means.

The term “vector” is used to refer to a DNA fragment(s) or nucleic acid molecule that is delivered into a cell. Vectors replicate DNA and can reproduce independently in host cells. The term “vector” is often used interchangeably with “vector”. The term “expression vector” refers to a recombinant DNA molecule containing the coding sequence of interest and the appropriate nucleic acid sequence necessary to express the operably linked coding sequence in a particular host organism.

Vectors of the invention can typically be constructed as vectors for cloning or expression. Additionally, the vector of the present invention can be constructed using prokaryotic cells or eukaryotic cells as hosts. For example, when the vector of the present invention is an expression vector and a prokaryotic cell is used as a host, a strong promoter capable of advancing transcription (e.g., pLλ promoter, trp promoter, lac promoter, T7 promoter, tac promoter, etc.), It typically includes a ribosome binding site for initiation of translation and a transcription/translation termination sequence. When Escherichia coli is used as a host cell, the promoter and operator regions of the E. coli tryptophan biosynthesis pathway and the left-handed promoter of phage λ (pLλ promoter) can be used as control regions.

Meanwhile, vectors that can be used in the present invention include plasmids often used in the art (e.g., pSC101, ColE1, pBR322, pUC8/9, pHC79, pGEX series, pET series, and pUC19, etc.), phages (e.g., λgt4·λB) , λ-Charon, λΔz1, and M13, etc.) or viruses (e.g., SV40, etc.).

On the other hand, when the vector of the present invention is an expression vector and uses a eukaryotic cell as a host, a promoter derived from the genome of a mammalian cell (e.g., metallothionein promoter) or a promoter derived from a mammalian virus (e.g., adenovirus) Viral late promoters, vaccinia virus 7.5K promoter, SV40 promoter, cytomegalovirus promoter and tk promoter of HSV) can be used and typically have a polyadenylation sequence as the transcription termination sequence.

The recombinant vector of the present invention is preferably a plant expression vector.

A preferred example of a plant expression vector is the Ti-plasmid vector, which is capable of transferring part of itself, the so-called T-region, into plant cells when present in a suitable host such as Agrobacterium tumefaciens . Other types of Ti-plasmid vectors (see EP 0 116 718 B1) are currently used to transfer hybrid DNA sequences into plant cells or protoplasts from which new plants can be produced that properly integrate the hybrid DNA into the plant's genome. there is. A particularly preferred form of Ti-plasmid vectors are the so-called binary vectors as claimed in EP 0 120 516 B1 and US Pat. No. 4,940,838. Other suitable vectors that can be used to introduce genes according to the invention into plant hosts include viral vectors such as those that may be derived from double-stranded plant viruses (e.g., CaMV) and single-stranded viruses, geminiviruses, etc. For example, it may be selected from non-intact plant virus vectors. The use of such vectors can be particularly advantageous when it is difficult to properly transform plant hosts.

The expression vector preferably contains one or more selectable markers. The marker is a nucleic acid sequence that has characteristics that can be generally selected by chemical methods, and includes all genes that can distinguish transformed cells from non-transformed cells. Examples include herbicide resistance genes such as glyphosate or phosphinothricin, antibiotics such as kanamycin, G418, bleomycin, hygromycin, and chloramphenicol. There is a resistance gene, but it is not limited to this.

In the plant expression vector according to the present invention, the promoter may be a small rubber particle-associated protein (SRPP), CaMV 35S, actin, ubiquitin, pEMU, MAS, or histone promoter, but is not limited thereto. The term "promoter" refers to the region of DNA upstream from a structural gene and refers to the DNA molecule to which RNA polymerase binds to initiate transcription. A “plant promoter” is a promoter capable of initiating transcription in plant cells. A “constitutive promoter” is a promoter that is active under most environmental conditions and developmental states or cell differentiation. Constitutive promoters may be preferred in the present invention because selection of transformants can be accomplished at various stages and by various tissues. Therefore, constitutive promoters do not limit selection possibilities.

In the plant expression vector according to the present invention, the terminator can be a conventional terminator, examples of which include nopaline synthase (NOS), rice α-amylase RAmy1 A terminator, phaseoline terminator, and Agrobacterium terminator. These include, but are not limited to, the terminator of the Octopine gene of Rhium tumefaciens.

The present invention also provides host cells transformed with the above recombinant vector.

Host cells capable of stably and continuously cloning and expressing the vector of the present invention can be any host cell known in the art, including microalgae, microorganisms, etc., such as E. coli JM109, E. coli BL21, Bacillus genus strains such as E. coli RR1, E. coli LE392, E. coli B , E. coli and Enterobacteriaceae strains such as Salmonella typhimurium, Serratia marcescens, and various Pseudomonas species.

In addition, when the vector of the present invention is transformed into eukaryotic cells, yeast (e.g., Saccharomyce cerevisiae ), insect cells, human cells (e.g., CHO cell line (Chinese hamster ovary), W138, BHK, COS-7) are used as host cells. , 293, HepG2, 3T3, RIN and MDCK cell lines) and plant cells can be used, preferably plant cells.

Methods for transporting the vector of the present invention into a host cell include, when the host cell is a prokaryotic cell, the CaCl ₂ method and the Hanahan method (Hanahan, D., J. Mol. Biol., 166:557-580 (1983)) and electroporation methods. In addition, when the host cell is a eukaryotic cell, the vector can be injected into the host cell by microinjection, calcium phosphate precipitation, electroporation, liposome-mediated transfection, DEAE-dextran treatment, and gene bombardment. You can.

The present invention also includes the steps of transforming a plant cell with a recombinant vector containing a corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2; and redifferentiating the transformed plant from the transformed plant cells.

Plant transformation refers to any method of transferring DNA to a plant. Such transformation methods do not necessarily require a regeneration and/or tissue culture period. Transformation of plant species is now common for plant species including both monocots as well as dicots. In principle, any transformation method can be used to introduce the hybrid DNA according to the invention into suitable progenitor cells. Methods include the calcium/polyethylene glycol method for protoplasts, electroporation of protoplasts, microinjection into plant elements, particle bombardment (DNA or RNA-coated) of various plant elements, invasion of plants or characterization of mature pollen or spores. It can be appropriately selected from Agrobacterium tumefaciens-mediated gene transfer by conversion, infection by (non-complete) virus, etc. A preferred method according to the invention involves Agrobacterium mediated DNA transfer. Particular preference is given to using the so-called binary vector technology as described in EP A 120 516 and US Pat. No. 4,940,838.

The method of the present invention includes the step of transforming a plant cell with a recombinant vector containing the corn-derived RCD1 gene consisting of the base sequence of SEQ ID NO: 1, wherein the transformation is carried out in Agrobacterium tumefaciens ( A. tumefaciens ). can be mediated by Additionally, the method of the present invention includes the step of redifferentiating a transformed plant from the transformed plant cell. A method for redifferentiating a transgenic plant from a transgenic plant cell can use any method known in the art.

The “plant cell” used for plant transformation can be any plant cell. The plant cells may be any form of cultured cells, cultured tissues, cultured organs or whole plants, preferably cultured cells, cultured tissues or cultured organs and more preferably cultured cells. “Plant tissue” refers to differentiated or undifferentiated plant tissues, such as, but not limited to, fruits, stems, leaves, pollen, seeds, cancer tissues, and various types of cells used in culture, such as single cells and protoplasts. (protoplast), shoot and callus tissue. Plant tissue may be in planta or in organ culture, tissue culture, or cell culture.

In the method for producing a transgenic plant according to an embodiment of the present invention, increasing the expression of the corn-derived RCD1 protein coding gene in the transformed plant cell results in a transgenic plant having increased drought stress resistance compared to a non-transgenic plant. can be manufactured.

The present invention also provides transgenic plants with controlled drought stress resistance prepared by the production method of the present invention and their transformed seeds.

As described above, the transgenic plant of the present invention is characterized by increased drought stress resistance when the expression of the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2 is increased.

In one embodiment of the present invention, the plant is a monocot plant such as corn, rice, barley, wheat, rye, sugarcane, oats, and onion, or Arabidopsis thaliana, potato, eggplant, tobacco, pepper, tomato, burdock, mugwort, It may be dicotyledonous plants such as lettuce, bellflower root, spinach, Swiss chard, sweet potato, celery, carrot, water parsley, parsley, Chinese cabbage, cabbage, mustard radish, watermelon, melon, cucumber, pumpkin, gourd, strawberry, soybean, mung bean, kidney bean, and pea. , but is not limited to this.

The present invention also provides drought stress resistance in plants, comprising the step of transforming plant cells with a recombinant vector containing the corn-derived RCD1 protein-coding gene consisting of the amino acid sequence of SEQ ID NO: 2 to regulate the expression of the RCD1 protein-coding gene. Provides a method to control .

In the method for controlling drought stress resistance in plants according to an embodiment of the present invention, when the expression of the corn-derived RCD1 protein coding gene is increased, drought stress resistance may be increased.

The present invention also provides a composition for controlling drought stress resistance in plants, comprising the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2 as an active ingredient. The composition of the present invention contains the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO. 2 as an active ingredient, and transforms plant cells with the gene or a recombinant vector containing the gene, thereby increasing the plant's resistance to drying stress. It can be adjusted.

Hereinafter, the present invention will be described in detail by examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

Example 1. Selection of drought-resistant corn plants

A seedling-stage drying resistance test was conducted on domestically grown corn lines distributed from the Corn Research Institute of the Gangwon Provincial Agricultural Research and Extension Services. Briefly, for the seedling season drought tolerance test, 30 seeds per line were divided into control and experimental groups and sown in 15 50-hole standard seedling pots using horticultural topsoil as soil. Immediately after germination, plants with poor vitality were thinned out and ultimately 40 plants per line were used for testing. The inter-day distance of the individuals within the pot was maintained at 5.5 cm and the inter-day distance at 11 cm, and the experiment was conducted at the DH facility house of the Corn Research Institute of the Gangwon Provincial Agricultural Research and Extension Services. To minimize stress other than drying, such as the high temperature inside the house, the side windows were opened during the day to circulate air, and a light shield was installed to prevent exposure to direct sunlight. Water was supplied twice a day to prevent the topsoil of the control and experimental groups from drying out. Drying treatment was performed when more than 50% of the individuals reached the third leaf stage. The control group continued to provide moisture, and the experimental group stopped supplying water until all the animals died. The survival rate after cutting was investigated, and if the survival rate was the same, the line with less collapse compared to the control was evaluated as the line with better drought tolerance in the seedling stage.

Based on the drying resistance numerical information, 5 lines (No. 6 to 10 in Table 1) that were very resistant to drying stress and 5 lines (No. 1 to 5 in Table 1) that were very weak to drying stress were finally selected (Figures 1 and 1). Table 1).

Information on 10 corn lines selected through drying resistance tests No. System name Survival rate (%) No. System name Survival rate (%) One 06S8130 25 6 07S8045 90 2 06S8140 15 7 04S8077 65 3 hf17 10 8 04S8093 65 4 hf19 10 9 05S10032 60 5 02S8072 5 10 B84 50

Example 2. Genome-wide SNP genotyping using a 50K corn SNP chip

Illumina's MaizeSNP50 BeadChip was manufactured based on the reference sequence of seed corn B73, and B73 was reported to be a strain with very weak drought resistance. MaizeSNP50 BeadChip contains a total of 56,110 SNPs molecular markers, and can test an average of more than 25 markers per megabase (Mb) of the corn genome. Using the SNP chip, the present inventors performed genome-wide SNP genotyping analysis on 5 lines that were very resistant to drying stress and 5 lines that were very weak to drying stress selected in Example 1. As a result, genotyping of a total of 42,863 SNPs was possible, and resistance/susceptibility SNPs were selected based on this information.

Example 3. Selection of SNPs related to desiccation resistance through linkage analysis

Selection of group- or individual-specific SNPs was done by first setting a comparison group according to traits and characteristics and then selecting SNPs that were conflicting between groups. In particular, among the conflicting SNPs, SNPs that exist in heterozygous form were excluded, and only homozygous SNPs were finally selected (Figure 2).

Based on the methodology described above, a desiccation-resistant or susceptible clade was set as the comparison group, and SNP locus that were identical within the group but conflicting between the two groups were selected. As a result, 133 SNP molecular markers associated with drought resistance or susceptibility were selected (Figure 3).

Example 4. Analysis of location information in the genome of 133 SNPs associated with corn drying stress resistance/susceptibility

Detailed location of 133 SNPs using bioinformatic techniques (BlastN and BlastX programs provided by NCBI and in vitro translation programs provided by SMS (The Sequence Manipulation Suite, https://bioinformatics.org/sms/)). Information analysis was performed. As a result, 51 SNPs were located in non-gene regions, 12 SNPs were located in exon sequences of genes for which information was unknown, 20 SNPs were located in intron sequences of genes, and 37 SNPs were located in regions with confirmed functions. It was confirmed that 12 SNPs were present in the exon, the protein coding region of the gene, and in the UTR (untranslated region) region of the gene. Among these, 37 SNPs located in genes with confirmed functions were investigated for SNPs that induce amino acid mutations. As a result, 19 SNPs did not cause amino acid mutations, while 18 SNPs caused amino acid mutations depending on DNA mutation. It was confirmed that it was being induced. Eighteen SNPs that exist in the exon sequences of genes with confirmed functions and induce amino acid mutations were finally selected as genetic factors related to corn drying stress (Figure 4).

Based on the in-silico functional analysis of the genes where the 18 finally selected SNPs are located and the results of previous research papers, 6 genes expected to be closely related to drying stress resistance were selected, and the information is shown in Table 2. .

Detailed information on genes related to corn drying stress resistance SNP Gene activity Susceptible Tolerant PZE-101189877 Glutamine synthase AA CC PZA02367.1 RCD1 GG AA SYN24806 Early flowering proteins CC AA SYN2723 Ring finger protein AA GG SYN4187 ARF CC AA PZE-108030790 MYB transcription factor AA GG

Example 5. Structural analysis of genes related to corn drying stress resistance

Gene structure analysis was performed on six selected corn drying stress-related genes (Figure 5). In addition, the structure and SNP were verified again by securing and comparing the cDNA sequences of the genes from the drought stress-resistant and susceptible lines. Although some selective transcripts were discovered, the structure of all six genes and the presence of SNPs were confirmed again.

Example 6. Functional verification of genes related to corn drying stress resistance

One of the six selected candidate SNPs is the 745th of the corn inactive poly [ADP-ribose] polymerase RCD1 gene, located in the first exon of the gene, and contains Aspartic Acid (D)/Asparagine (N) It was confirmed that mutations were created (Figure 6). In addition, as a result of analyzing the base sequence of the RCD1 gene of corn from lines resistant or sensitive to drying stress, base mutations leading to amino acid substitutions were confirmed in addition to the above SNPs (FIG. 6). It has been reported that the RCD1 gene is essential for abiotic stress response and can regulate various stress response genes.

The present inventors separately isolated transcripts ( RCD1 and rcd1 ) from drought stress-resistant and sensitive lines, respectively, to construct plant expression vectors, and to verify their function by introducing them into Arabidopsis plants. For the construction of plant expression vectors, the full-length sequence of the maize RCD1 gene (Genbank accession number: : 5'-AAAAAAGCAGGCTTGATGGAACAGAGGAATGTTCTGGCA-3' (SEQ ID NO: 4) and reverse-attB2: 5'-AGAAAGCTGGGGTATTACTCGGCTACTCCCCCTCCTCTT-3' (SEQ ID NO: 5). After the second round of PCR using an adapter primer set (attB1-adapter: 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCT-3' (SEQ ID NO. 6) and attB2-adapter: 5'-GGGGACCACTTTGTACAAGAAAGCTGGGT-3' (SEQ ID NO. 7)), the amplified product was subcloned into the pDONOR221 vector using BP clonase II (Invitrogen, USA) according to the manufacturer's instructions. The recombinant vector was transformed into E. coli TOP10 cells (Invitrogen), transformants were selected through kanamycin resistance, and the isolated plasmid was confirmed through sequencing analysis. The gene construct in the pDONOR221 vector was inserted into the pB7FWG2 binary destination vector using Gateway LR Clonase II Plus Enzyme Mix (Invitrogen). The clone was transformed into E. coli TOP10 cells, transformants were selected using spectinomycin, and the final expression vector (pB7FWG2 harboring ZmRCD1 ) was prepared. The vector construct regulates the expression of the ZmRCD1 gene by the CaMV 35S promoter and contains the bar gene as a selection marker. The recombinant vector was introduced into Arabidopsis plants through an Agrobacterium-mediated transformation method, and the drought stress resistance of the transformed plants was confirmed using the same method as disclosed in Example 1.

As a result, it was confirmed that Arabidopsis plants into which the ZmRCD1 gene (SEQ ID NO: 1) expressed in a drought-resistant line was introduced had resistance in a dry stress environment (FIG. 7). Based on the above results, it was expected that the ZmRCD1 gene derived from the dry stress resistant line could be used to develop new corn dry stress resistant varieties.

In addition, the present inventor has developed a molecular marker that can identify the SNP located at position 745 of the ZmRCD1 gene, and plans to use the molecular marker to develop new varieties as a marker for determining drought stress resistance and susceptible corn resources.

Sequence list electronic file attached

Claims (12)

Corn-derived RCD1 protein consisting of the amino acid sequence of SEQ ID NO: 2. A gene encoding the RCD1 protein of claim 1. The gene according to claim 2, wherein the gene consists of the base sequence of SEQ ID NO: 1. A recombinant vector containing the gene of claim 2. A host cell transformed with the recombinant vector of claim 4. The host cell according to claim 5, wherein the host cell is a plant. Transforming a plant cell with a recombinant vector containing the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2; and
A method for producing a transformed plant with controlled drought stress resistance, comprising the step of redifferentiating the transformed plant from the transformed plant cell. The method of claim 7, wherein drying stress resistance is increased compared to non-transgenic plants by increasing the expression of the corn-derived RCD1 protein coding gene. A transgenic plant with controlled drought stress resistance prepared by the method of claim 7 or 8. A transformed seed of the plant according to claim 9. A method for controlling drought stress resistance of a plant, comprising transforming a plant cell with a recombinant vector containing the corn-derived RCD1 protein-coding gene consisting of the amino acid sequence of SEQ ID NO: 2 and controlling the expression of the RCD1 protein-coding gene. A composition for controlling drought stress resistance in plants, comprising as an active ingredient the corn-derived RCD1 protein coding gene consisting of the amino acid sequence of SEQ ID NO: 2.